Rorug04G0274100

PKHD-type hydroxylase At1g22950-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0004916
Physical Location & Seq
Reverse (-)
44638304 .. 44638804
501 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0274100.1

Sequence Viewer

Length: 387 bp
ATGGGTGTCTTCACATATGAAACTGAGTTCACCTCTGTAATCCCACCACCAAGATTGTTCAAGGCCTTTGTTCTTGATGCTGACAACCTTATCCCCAATATTGCCCCACAAGCTGTCAAGAGTGCTGAAATCGTTGAAGGGGATGGAGGTGTTGGAACCATCAAGAAGATTAACCTCGGGGAAGGAAGTGAATACAGCTATGTGAAGCACAAGATTGAAAGCCTTGACAAAGACCACTTTGTTTACAGCTATAGTTTGATTGAAGGCGATGCTATTGGAGACAAAATCGAGAAGATCTCTTATGAGATTAAGTTGGTTGCATCTGCTGATGGAGGTTCTATCATAAAGAGCAGCAGCAACTACCATTGCAAAAGTGATCGAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

128

Amino Acids

13.93

Weight (kDa)

5.14

Isoelectric Point (pI)

27.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 126 5.7e-21 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 4 cut(s) 61, 137, 218, 263
AluBI AGCT 3 cut(s) 113, 198, 249
AluI AGCT 3 cut(s) 113, 198, 249
Alw26I GTCTC 1 cut(s) 273
Ama87I CYCGRG 1 cut(s) 176
AoxI GGCC 1 cut(s) 63
ApeKI GCWGC 2 cut(s) 351, 354
AsuHPI GGTGA 1 cut(s) 22
AvaI CYCGRG 1 cut(s) 176
BbvI GCAGC 2 cut(s) 363, 366
BccI CCATC 3 cut(s) 137, 167, 323
BcoDI GTCTC 1 cut(s) 273
BfmI CTRYAG 1 cut(s) 250
BglII AGATCT 1 cut(s) 294
BisI GCNGC 2 cut(s) 352, 355
BlsI GCNGC 2 cut(s) 353, 356
BmeT110I CYCGRG 1 cut(s) 176
BmiI GGNNCC 1 cut(s) 157
BmsI GCATC 3 cut(s) 67, 259, 329
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 281, 313
BsaJI CCNNGG 1 cut(s) 175
Bse3DI GCAATG 1 cut(s) 364
BseDI CCNNGG 1 cut(s) 175
BseGI GGATG 1 cut(s) 148
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 1 cut(s) 15
BseXI GCAGC 2 cut(s) 363, 366
BshFI GGCC 1 cut(s) 65
BsiHKCI CYCGRG 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 273
BsnI GGCC 1 cut(s) 65
BsoBI CYCGRG 1 cut(s) 176
Bsp143I GATC 2 cut(s) 294, 376
BspANI GGCC 1 cut(s) 65
BspCNI CTCAG 1 cut(s) 16
BspLI GGNNCC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 364
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 2 cut(s) 294, 376
BstDEI CTNAG 1 cut(s) 24
BstF5I GGATG 1 cut(s) 148
BstKTI GATC 2 cut(s) 297, 379
BstMAI GTCTC 1 cut(s) 273
BstMBI GATC 2 cut(s) 294, 376
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 250
BstV1I GCAGC 2 cut(s) 363, 366
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuRI GGCC 1 cut(s) 65
BtgZI GCGATG 1 cut(s) 282
BtsCI GGATG 1 cut(s) 148
CspCI CAANNNNNGTGG 2 cut(s) 36, 71
CviJI RGCY 5 cut(s) 65, 113, 198, 222, 249
CviKI_1 RGCY 5 cut(s) 65, 113, 198, 222, 249
DdeI CTNAG 1 cut(s) 24
DpnI GATC 2 cut(s) 296, 378
DpnII GATC 2 cut(s) 294, 376
Eco147I AGGCCT 1 cut(s) 65
Eco88I CYCGRG 1 cut(s) 176
FaiI YATR 6 cut(s) 16, 18, 201, 252, 303, 344
FauNDI CATATG 1 cut(s) 16
Fnu4HI GCNGC 2 cut(s) 352, 355
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 2 cut(s) 352, 355
GluI GCNGC 2 cut(s) 352, 355
HaeIII GGCC 1 cut(s) 65
HphI GGTGA 1 cut(s) 22
Hpy166II GTNNAC 2 cut(s) 30, 244
Hpy188III TCNNGA 4 cut(s) 74, 118, 163, 289
Hpy8I GTNNAC 2 cut(s) 30, 244
HpyAV CCTTC 3 cut(s) 131, 176, 257
HpyCH4V TGCA 2 cut(s) 320, 369
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 1 cut(s) 24
Kzo9I GATC 2 cut(s) 294, 376
Lsp1109I GCAGC 2 cut(s) 363, 366
LweI GCATC 3 cut(s) 67, 259, 329
MalI GATC 2 cut(s) 296, 378
MboI GATC 2 cut(s) 294, 376
MboII GAAGA 2 cut(s) 178, 304
MflI RGATCY 1 cut(s) 294
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 5 cut(s) 43, 140, 185, 326, 374
MseI TTAA 2 cut(s) 171, 309
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeI CATATG 1 cut(s) 16
NdeII GATC 2 cut(s) 294, 376
NlaIV GGNNCC 1 cut(s) 157
PceI AGGCCT 1 cut(s) 65
PkrI GCNGC 2 cut(s) 353, 356
PspN4I GGNNCC 1 cut(s) 157
PsuI RGATCY 1 cut(s) 294
SaqAI TTAA 2 cut(s) 171, 309
SatI GCNGC 2 cut(s) 352, 355
Sau3AI GATC 2 cut(s) 294, 376
SetI ASST 9 cut(s) 35, 90, 115, 151, 177, 200, 251, 337, 385
SfaNI GCATC 3 cut(s) 67, 259, 329
SfcI CTRYAG 1 cut(s) 250
SseBI AGGCCT 1 cut(s) 65
SspI AATATT 1 cut(s) 100
StuI AGGCCT 1 cut(s) 65
TaqI TCGA 2 cut(s) 288, 379
Tru1I TTAA 2 cut(s) 171, 309
Tru9I TTAA 2 cut(s) 171, 309
TseI GCWGC 2 cut(s) 351, 354
TspDTI ATGAA 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.