AT1G23390

kelch repeat-containing F-box family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010437
Physical Location & Seq
Reverse (-)
8308965 .. 8310916
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23390.1

Sequence Viewer

Length: 1185 bp
ATGGAGAAGAAGAAGAACAACAACAATGGAGGAGATTTCGGAGAAGAAGAAAGTTCCATTGACGGAGACATACTCGAGTCAATCCTCTCCTATTTACCACTCCTAGACCTCGATTCCGCTTGCCAAGTCTCCAAATCATGGAACCGCGCCGTCTTTTACTCCCTCCGCCGCCTTAAAACCATGCCGTGGTTGTTCGTCTACAACCAGAGAAATTCTCCGCCGTACACCATGGCGACGATGGCGATGGCTTATGATCCCAAGTCCGAGGCCTGGATCGAGCTCAACACCGCATCATCTCCCGTCGAACACGTCTCCGTTGCTCGATCCTCTCACTCCACGTTGCTCTACGCTTTATCTCCGGCGAGATTCTCTTTCTCAACCGACGCGTTTCATCTGACTTGGCAACACGTGGCACCGCCGAGAGTTTGGAGGATCGATCCAATCGTGGCTGTAGTCGGACGGAGCCTGATTATAGCCGGAGGTGTGTGCGATTTCGAGGAGGATAGATTCGCCGTGGAGCTGTTCGACATCGAATCCGGAGACGGAGCGTGGGAGAGATGCGAATCGATGCCAGATTTTCTCTACGAATCCGCGTCGTCCACGTGGCTTTCCGTAGCGGTGAGTTCGGAGAAGATGTACGTGACGGAGAAGAGATCAGGCGTTACGTGTAGTTTCAATCCAGTAACTAGATCGTGGACGAAGCTTCTCGATCTCTGTCCCGGCGAGTGTAGCTTATACTCACGTTCGATTGGATTCTCCGTTAATCGTTTAATTATGGCTGGGATCATCGGAGATGAATATAATCCGACGGGAATCGAACTGTGGGAAGTTATAGATTCTGACGAATCGCATTTGAAATTCGAGTCGATCGGATCTATGCCTGAGACGTATTTGGAGAAACTAAGAGGGATTAATTCTGATTGGCCGTTAACGTCGATAGTGTTGAACGCGGTGGGAGATATGGTTTATGTACATGGTGCGGCGGAGAACGGCGGAGAGATTGTTGCGGCGGAGATTGAAGGAGGAAAGCTTTGTAAGTGGAGGACTTTGCCAAACGCAGACGCTACTTGGAAAAAAAGTCACGCGGCTGAGAGAGTAATCGTTGCGTGTTCAAACGTTGGTTTTAGCGATTTGAAACTTGCGTTTAGGAATAATTTGAGTTTTCTCTCAACGTCAAAATATTGA

Protein Analysis

394

Amino Acids

43.88

Weight (kDa)

5.0

Isoelectric Point (pI)

48.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 23 - 52 6.5e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 412
AccB7I CCANNNNNTGG 2 cut(s) 138, 186
AccI GTMKAC 1 cut(s) 198
AccII CGCG 5 cut(s) 147, 386, 593, 950, 1085
AccIII TCCGGA 1 cut(s) 536
AclI AACGTT 1 cut(s) 1116
AclWI GGATC 7 cut(s) 248, 281, 318, 431, 440, 791, 880
AcoI YGGCCR 1 cut(s) 923
AcsI RAATTY 2 cut(s) 211, 857
AcvI CACGTG 2 cut(s) 409, 603
AfaI GTAC 3 cut(s) 224, 638, 972
AfiI CCNNNNNNNGG 3 cut(s) 138, 186, 270
AflIII ACRYGT 4 cut(s) 307, 384, 406, 665
AgsI TTSAA 6 cut(s) 676, 856, 946, 1019, 1113, 1135
AjiI CACGTC 1 cut(s) 310
AjnI CCWGG 1 cut(s) 269
AluBI AGCT 5 cut(s) 280, 520, 703, 732, 1030
AluI AGCT 5 cut(s) 280, 520, 703, 732, 1030
Alw21I GWGCWC 1 cut(s) 282
Alw26I GTCTC 5 cut(s) 60, 133, 316, 534, 878
AlwI GGATC 7 cut(s) 248, 281, 318, 431, 440, 791, 880
Ama87I CYCGRG 1 cut(s) 74
Aor13HI TCCGGA 1 cut(s) 536
AoxI GGCC 2 cut(s) 267, 923
ApoI RAATTY 2 cut(s) 211, 857
AseI ATTAAT 1 cut(s) 912
AspLEI GCGC 1 cut(s) 149
AsuC2I CCSGG 1 cut(s) 720
AsuHPI GGTGA 1 cut(s) 631
AvaI CYCGRG 1 cut(s) 74
BanI GGYRCC 1 cut(s) 412
BanII GRGCYC 1 cut(s) 282
BbrPI CACGTG 2 cut(s) 409, 603
Bbv12I GWGCWC 1 cut(s) 282
BccI CCATC 2 cut(s) 232, 238
BceAI ACGGC 6 cut(s) 134, 169, 205, 497, 910, 1006
BciT130I CCWGG 1 cut(s) 271
BcnI CCSGG 1 cut(s) 720
BcoDI GTCTC 5 cut(s) 60, 133, 316, 534, 878
BfaI CTAG 2 cut(s) 104, 687
BfmI CTRYAG 1 cut(s) 450
BisI GCNGC 4 cut(s) 169, 981, 1008, 1086
BlsI GCNGC 4 cut(s) 170, 982, 1009, 1087
Bme1390I CCNGG 2 cut(s) 271, 720
BmeT110I CYCGRG 1 cut(s) 74
BmgBI CACGTC 1 cut(s) 310
BmiI GGNNCC 3 cut(s) 143, 414, 464
BmrFI CCNGG 2 cut(s) 271, 720
BmsI GCATC 3 cut(s) 299, 548, 558
BplI GAGNNNNNCTC 4 cut(s) 57, 89, 199, 231
BpuMI CCSGG 1 cut(s) 720
Bsa29I ATCGAT 2 cut(s) 435, 566
BsaAI YACGTR 4 cut(s) 409, 603, 640, 666
BsaBI GATNNNNATC 1 cut(s) 562
BsaJI CCNNGG 4 cut(s) 185, 228, 264, 513
BsaWI WCCGGW 1 cut(s) 536
BsaXI ACNNNNNCTCC 6 cut(s) 620, 650, 948, 978, 987, 1017
Bsc4I CCNNNNNNNGG 3 cut(s) 138, 186, 270
Bse1I ACTGG 1 cut(s) 680
Bse8I GATNNNNATC 1 cut(s) 562
BseAI TCCGGA 1 cut(s) 536
BseBI CCWGG 1 cut(s) 271
BseCI ATCGAT 2 cut(s) 435, 566
BseDI CCNNGG 4 cut(s) 185, 228, 264, 513
BseJI GATNNNNATC 1 cut(s) 562
BseLI CCNNNNNNNGG 3 cut(s) 138, 186, 270
BseMII CTCAG 2 cut(s) 873, 1080
BseNI ACTGG 1 cut(s) 680
BseRI GAGGAG 2 cut(s) 45, 512
BseYI CCCAGC 1 cut(s) 779
Bsh1236I CGCG 5 cut(s) 147, 386, 593, 950, 1085
Bsh1285I CGRYCG 1 cut(s) 870
BshFI GGCC 2 cut(s) 269, 925
BshNI GGYRCC 1 cut(s) 412
BshVI ATCGAT 2 cut(s) 435, 566
BsiEI CGRYCG 1 cut(s) 870
BsiHKAI GWGCWC 1 cut(s) 282
BsiHKCI CYCGRG 1 cut(s) 74
BsiSI CCGG 4 cut(s) 359, 477, 537, 720
BslFI GGGAC 1 cut(s) 702
BslI CCNNNNNNNGG 3 cut(s) 138, 186, 270
BsmAI GTCTC 5 cut(s) 60, 133, 316, 534, 878
BsmBI CGTCTC 3 cut(s) 316, 534, 878
BsmFI GGGAC 1 cut(s) 702
BsnI GGCC 2 cut(s) 269, 925
BsoBI CYCGRG 1 cut(s) 74
Bsp1286I GDGCHC 1 cut(s) 282
Bsp13I TCCGGA 1 cut(s) 536
Bsp1407I TGTACA 1 cut(s) 970
Bsp19I CCATGG 1 cut(s) 228
BspANI GGCC 2 cut(s) 269, 925
BspCNI CTCAG 2 cut(s) 874, 1081
BspDI ATCGAT 2 cut(s) 435, 566
BspEI TCCGGA 1 cut(s) 536
BspFNI CGCG 5 cut(s) 147, 386, 593, 950, 1085
BspLI GGNNCC 3 cut(s) 143, 414, 464
BspPI GGATC 7 cut(s) 248, 281, 318, 431, 440, 791, 880
BspT107I GGYRCC 1 cut(s) 412
BsrGI TGTACA 1 cut(s) 970
BsrI ACTGG 1 cut(s) 680
BssECI CCNNGG 4 cut(s) 185, 228, 264, 513
BssT1I CCWWGG 1 cut(s) 228
Bst2UI CCWGG 1 cut(s) 271
Bst4CI ACNGT 1 cut(s) 822
Bst6I CTCTTC 1 cut(s) 644
BstAUI TGTACA 1 cut(s) 970
BstBAI YACGTR 4 cut(s) 409, 603, 640, 666
BstC8I GCNNGC 1 cut(s) 121
BstDEI CTNAG 3 cut(s) 882, 902, 1089
BstDSI CCRYGG 3 cut(s) 185, 228, 513
BstFNI CGCG 5 cut(s) 147, 386, 593, 950, 1085
BstHHI GCGC 1 cut(s) 149
BstMAI GTCTC 5 cut(s) 60, 133, 316, 534, 878
BstMCI CGRYCG 1 cut(s) 870
BstMWI GCNNNNNNNGC 2 cut(s) 239, 729
BstNI CCWGG 1 cut(s) 271
BstSCI CCNGG 2 cut(s) 269, 718
BstSFI CTRYAG 1 cut(s) 450
BstUI CGCG 5 cut(s) 147, 386, 593, 950, 1085
BstX2I RGATCY 1 cut(s) 872
BstYI RGATCY 1 cut(s) 872
Bsu15I ATCGAT 2 cut(s) 435, 566
BsuRI GGCC 2 cut(s) 269, 925
BsuTUI ATCGAT 2 cut(s) 435, 566
BtgI CCRYGG 3 cut(s) 185, 228, 513
BtgZI GCGATG 1 cut(s) 257
BtrI CACGTC 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 121
CfoI GCGC 1 cut(s) 149
ClaI ATCGAT 2 cut(s) 435, 566
CseI GACGC 3 cut(s) 392, 582, 1070
Csp6I GTAC 3 cut(s) 223, 637, 971
CviAII CATG 4 cut(s) 138, 181, 229, 974
CviQI GTAC 3 cut(s) 223, 637, 971
DdeI CTNAG 3 cut(s) 882, 902, 1089
EaeI YGGCCR 1 cut(s) 923
Eam1104I CTCTTC 1 cut(s) 644
EarI CTCTTC 1 cut(s) 644
EciI GGCGGA 5 cut(s) 155, 207, 998, 1008, 1025
Ecl136II GAGCTC 1 cut(s) 280
Eco130I CCWWGG 1 cut(s) 228
Eco147I AGGCCT 1 cut(s) 269
Eco24I GRGCYC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 280
Eco72I CACGTG 2 cut(s) 409, 603
Eco88I CYCGRG 1 cut(s) 74
EcoICRI GAGCTC 1 cut(s) 280
EcoRII CCWGG 1 cut(s) 269
EcoT14I CCWWGG 1 cut(s) 228
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 228
Esp3I CGTCTC 3 cut(s) 316, 534, 878
FaeI CATG 4 cut(s) 141, 184, 232, 977
FaqI GGGAC 1 cut(s) 702
FatI CATG 4 cut(s) 137, 180, 228, 973
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 4 cut(s) 169, 981, 1008, 1086
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 4 cut(s) 169, 981, 1008, 1086
FspBI CTAG 2 cut(s) 104, 687
GlaI GCGC 1 cut(s) 148
GluI GCNGC 4 cut(s) 169, 981, 1008, 1086
GsaI CCCAGC 1 cut(s) 783
HaeIII GGCC 2 cut(s) 269, 925
HapII CCGG 4 cut(s) 359, 477, 537, 720
HgaI GACGC 3 cut(s) 392, 582, 1070
HhaI GCGC 1 cut(s) 149
Hin1II CATG 4 cut(s) 141, 184, 232, 977
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 930
HindII GTYRAC 1 cut(s) 930
HindIII AAGCTT 2 cut(s) 701, 1028
HpaI GTTAAC 1 cut(s) 930
HpaII CCGG 4 cut(s) 359, 477, 537, 720
HphI GGTGA 1 cut(s) 631
Hpy166II GTNNAC 5 cut(s) 199, 225, 600, 696, 930
Hpy188III TCNNGA 2 cut(s) 537, 707
Hpy8I GTNNAC 5 cut(s) 199, 225, 600, 696, 930
Hpy99I CGWCG 6 cut(s) 238, 305, 386, 598, 811, 937
HpyAV CCTTC 1 cut(s) 1013
HpyCH4III ACNGT 1 cut(s) 822
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 729
HpyF3I CTNAG 3 cut(s) 882, 902, 1089
Hsp92II CATG 4 cut(s) 141, 184, 232, 977
HspAI GCGC 1 cut(s) 147
Kpn2I TCCGGA 1 cut(s) 536
KspAI GTTAAC 1 cut(s) 930
LmnI GCTCC 3 cut(s) 462, 517, 545
LweI GCATC 3 cut(s) 299, 548, 558
MaeI CTAG 2 cut(s) 104, 687
MaeIII GTNAC 4 cut(s) 640, 661, 682, 1079
MboII GAAGA 7 cut(s) 19, 22, 25, 56, 59, 643, 661
MflI RGATCY 1 cut(s) 872
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 5 cut(s) 211, 771, 857, 913, 1153
MluI ACGCGT 1 cut(s) 384
MlyI GAGTC 2 cut(s) 86, 872
MmeI TCCRAC 2 cut(s) 436, 830
MroI TCCGGA 1 cut(s) 536
MseI TTAA 5 cut(s) 174, 762, 770, 912, 929
MspI CCGG 4 cut(s) 359, 477, 537, 720
MspR9I CCNGG 2 cut(s) 271, 720
MvaI CCWGG 1 cut(s) 271
MvnI CGCG 5 cut(s) 147, 386, 593, 950, 1085
MwoI GCNNNNNNNGC 2 cut(s) 239, 729
NciI CCSGG 1 cut(s) 720
NcoI CCATGG 1 cut(s) 228
NlaIII CATG 4 cut(s) 141, 184, 232, 977
NlaIV GGNNCC 3 cut(s) 143, 414, 464
NmeAIII GCCGAG 1 cut(s) 444
NmuCI GTSAC 2 cut(s) 640, 1079
PaeR7I CTCGAG 1 cut(s) 74
PceI AGGCCT 1 cut(s) 269
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PflMI CCANNNNNTGG 2 cut(s) 138, 186
PkrI GCNGC 4 cut(s) 170, 982, 1009, 1087
Ple19I CGATCG 1 cut(s) 870
PleI GAGTC 2 cut(s) 85, 871
PmaCI CACGTG 2 cut(s) 409, 603
PmlI CACGTG 2 cut(s) 409, 603
PpsI GAGTC 2 cut(s) 85, 871
Ppu21I YACGTR 4 cut(s) 409, 603, 640, 666
PshBI ATTAAT 1 cut(s) 912
Psp124BI GAGCTC 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 1116
Psp6I CCWGG 1 cut(s) 269
PspCI CACGTG 2 cut(s) 409, 603
PspFI CCCAGC 1 cut(s) 779
PspGI CCWGG 1 cut(s) 269
PspN4I GGNNCC 3 cut(s) 143, 414, 464
PspXI VCTCGAGB 1 cut(s) 74
PsuI RGATCY 1 cut(s) 872
PvuI CGATCG 1 cut(s) 870
RsaI GTAC 3 cut(s) 224, 638, 972
RsaNI GTAC 3 cut(s) 223, 637, 971
SacI GAGCTC 1 cut(s) 282
SaqAI TTAA 5 cut(s) 174, 762, 770, 912, 929
SatI GCNGC 4 cut(s) 169, 981, 1008, 1086
SchI GAGTC 2 cut(s) 86, 872
ScrFI CCNGG 2 cut(s) 271, 720
SduI GDGCHC 1 cut(s) 282
SfaNI GCATC 3 cut(s) 299, 548, 558
SfcI CTRYAG 1 cut(s) 450
Sfr274I CTCGAG 1 cut(s) 74
SlaI CTCGAG 1 cut(s) 74
SmlI CTYRAG 1 cut(s) 74
SmoI CTYRAG 1 cut(s) 74
Sse9I AATT 5 cut(s) 211, 771, 857, 913, 1153
SseBI AGGCCT 1 cut(s) 269
SspI AATATT 1 cut(s) 1181
SspMI CTAG 2 cut(s) 104, 687
SstI GAGCTC 1 cut(s) 282
StuI AGGCCT 1 cut(s) 269
StyD4I CCNGG 2 cut(s) 269, 718
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 1 cut(s) 822
TasI AATT 5 cut(s) 211, 771, 857, 913, 1153
TatI WGTACW 1 cut(s) 970
TauI GCSGC 4 cut(s) 171, 983, 1010, 1088
Tru1I TTAA 5 cut(s) 174, 762, 770, 912, 929
Tru9I TTAA 5 cut(s) 174, 762, 770, 912, 929
TseFI GTSAC 2 cut(s) 640, 1079
Tsp45I GTSAC 2 cut(s) 640, 1079
TspDTI ATGAA 2 cut(s) 380, 810
TspGWI ACGGA 7 cut(s) 78, 304, 475, 558, 601, 659, 748
Van91I CCANNNNNTGG 2 cut(s) 138, 186
VspI ATTAAT 1 cut(s) 912
XapI RAATTY 2 cut(s) 211, 857
XcmI CCANNNNNNNNNTGG 1 cut(s) 235
XhoI CTCGAG 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 2 cut(s) 104, 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.