RLG00000006052

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010437
Physical Location & Seq
Reverse (-)
3895086 .. 3896246
1161 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006052

Sequence Viewer

Length: 1161 bp
ATGGCAGTAACGGCGAACTCTAAGCAGAACCAGACCAAGGCGGCGGTTGAAGAAGAAGCACCGCTCTACGGAGACGTCTTGGAGTCCGTGCTATCCAAATTACACCTCATCCATCTCCTATCGGCGTGTCACGTCTCAAAGTCTTGGAACCGCGCCGTTTCGTCTACCCTCCGTTTCTCTAACCGAGTTAAACCCTGGCTCGTCGTCCACACTCAGACCAACAGGTTCCCCTACGTCGTCTCCGCACGTGCTTTCGACCCGGCCTCGCACGTGTGGGTCGACGTTGAACGGAGTCTTCATCATCCTCCGGTCAAGTCCATCTCCACCCTACGCTCCTCTCATTCGACTCTTCTATACATGCTTTCCCCTTCCAAATTCGCTTTCTCCCTCGACGCACTCCACCTGGAGTGGAGCCACGCAAACACGCCCCCACTCGTGTGGAGAACTGACCCGATTGTCGCCGTGGTGGGCCACCGCGTAATCGTCGCAGGCGGCGCGTGCGACTACGAGGAAGACCCGCTGGCCGTGGAAATGTACGATGTCAAAGTTGGCACGTGGGACACGTGCGAGTCCATGCCCGCCATTTTCAAAGACTCTTCGGCGTCCACGTGGCTGTCGGTGGCCGCGGACGAGAGGAGAATGTACGTGACGGAGAAGTGCTCGGCTGTCACGTACTCGTTTGATCCGGAGTCCAAGAAGTGGTTTGGGCCGTACGATCTGCGTCCGAATCCGACGGTGTTTAGCTCCGTGATTGGATTCGTCAACGGGCGTTTAGTTTTGGCGGGAGTTGTGGGAGAGGCGGAGGACGTGAAGGGAGTGAAGTTGTGGGAAGTGGAAGTCAGCGACGACGAGTCGTTGGAGTTGAAAGAGATGATCGGTGACATGCCGGAGACAATGGTGGAGAAGTTGAAGGGCGAGAGCCACTGTGAGCAGCCGTTTTCGATGAGCTATATGGGAGGTCTGGTGTGTTTTCACAACGGTTCCGACCCGAGCGAGTTGATCCTCTGCGAGCTTGAGAATGGGGGGTGCAGGTGGACGAGCATAAGGAATGACGCCATGAACGACGGGACCCGAATGCAGAGGATGGTGGTGACGTGTGCCAATCTGGGGTTGCCGGAGTTGCAGAAAGCCGTACAAGTCGGAGCACTGAAAATTCTATGA

Protein Analysis

387

Amino Acids

42.83

Weight (kDa)

5.56

Isoelectric Point (pI)

41.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 122 - 234 4.3e-07 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1020
AasI GACNNNNNNGTC 1 cut(s) 455
AatII GACGTC 1 cut(s) 78
Acc36I ACCTGC 1 cut(s) 1020
AccB7I CCANNNNNTGG 1 cut(s) 699
AccBSI CCGCTC 1 cut(s) 64
AccI GTMKAC 2 cut(s) 164, 279
AccII CGCG 4 cut(s) 153, 477, 497, 626
AccIII TCCGGA 1 cut(s) 685
AclWI GGATC 2 cut(s) 677, 994
AcoI YGGCCR 2 cut(s) 522, 621
AcsI RAATTY 2 cut(s) 374, 1152
AcvI CACGTG 5 cut(s) 248, 271, 555, 564, 609
AcyI GRCGYC 3 cut(s) 75, 602, 1053
AfaI GTAC 5 cut(s) 536, 644, 674, 713, 1134
AfiI CCNNNNNNNGG 4 cut(s) 37, 68, 699, 1107
AflIII ACRYGT 3 cut(s) 270, 561, 1094
AgsI TTSAA 5 cut(s) 50, 287, 589, 865, 910
AhdI GACNNNNNGTC 1 cut(s) 850
AjiI CACGTC 3 cut(s) 133, 808, 1095
AjnI CCWGG 2 cut(s) 194, 402
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AleI CACNNNNGTG 1 cut(s) 436
AluBI AGCT 3 cut(s) 744, 948, 1012
AluI AGCT 3 cut(s) 744, 948, 1012
Alw21I GWGCWC 2 cut(s) 662, 1147
Alw26I GTCTC 4 cut(s) 66, 139, 244, 884
AlwI GGATC 2 cut(s) 677, 994
Ama87I CYCGRG 1 cut(s) 988
Aor13HI TCCGGA 1 cut(s) 685
AoxI GGCC 5 cut(s) 261, 469, 522, 621, 707
ApeKI GCWGC 1 cut(s) 931
ApoI RAATTY 2 cut(s) 374, 1152
AspLEI GCGC 2 cut(s) 155, 497
AspS9I GGNCC 3 cut(s) 469, 707, 1068
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 2 cut(s) 890, 1102
AvaI CYCGRG 1 cut(s) 988
AvaII GGWCC 1 cut(s) 1068
BauI CACGAG 1 cut(s) 434
BbrPI CACGTG 5 cut(s) 248, 271, 555, 564, 609
BbsI GAAGAC 2 cut(s) 287, 519
Bbv12I GWGCWC 2 cut(s) 662, 1147
BbvI GCAGC 1 cut(s) 943
BccI CCATC 3 cut(s) 120, 326, 1078
BceAI ACGGC 7 cut(s) 27, 140, 446, 509, 694, 919, 1115
BciT130I CCWGG 2 cut(s) 196, 404
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 4 cut(s) 66, 139, 244, 884
BfuAI ACCTGC 1 cut(s) 1020
BisI GCNGC 4 cut(s) 42, 493, 624, 932
BlsI GCNGC 4 cut(s) 43, 494, 625, 933
Bme1390I CCNGG 3 cut(s) 196, 260, 404
Bme18I GGWCC 1 cut(s) 1068
BmeRI GACNNNNNGTC 1 cut(s) 850
BmeT110I CYCGRG 1 cut(s) 988
BmgBI CACGTC 3 cut(s) 133, 808, 1095
BmgT120I GGNCC 3 cut(s) 469, 707, 1068
BmiI GGNNCC 6 cut(s) 149, 227, 413, 982, 1069, 1070
BmrFI CCNGG 3 cut(s) 196, 260, 404
BpiI GAAGAC 2 cut(s) 287, 519
BplI GAGNNNNNCTC 2 cut(s) 644, 676
BpmI CTGGAG 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 1034
BpuMI CCSGG 1 cut(s) 260
BsaAI YACGTR 7 cut(s) 248, 271, 555, 564, 609, 646, 672
BsaHI GRCGYC 3 cut(s) 75, 602, 1053
BsaJI CCNNGG 5 cut(s) 36, 194, 462, 525, 624
BsaWI WCCGGW 2 cut(s) 307, 685
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 948, 978
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 68, 699, 1107
BseAI TCCGGA 1 cut(s) 685
BseBI CCWGG 2 cut(s) 196, 404
BseDI CCNNGG 5 cut(s) 36, 194, 462, 525, 624
BseGI GGATG 3 cut(s) 108, 301, 1089
BseLI CCNNNNNNNGG 4 cut(s) 37, 68, 699, 1107
BseMII CTCAG 1 cut(s) 227
BseRI GAGGAG 2 cut(s) 325, 649
BseXI GCAGC 1 cut(s) 943
BsgI GTGCAG 1 cut(s) 1048
Bsh1236I CGCG 4 cut(s) 153, 477, 497, 626
BshFI GGCC 5 cut(s) 263, 471, 524, 623, 709
BsiHKAI GWGCWC 2 cut(s) 662, 1147
BsiHKCI CYCGRG 1 cut(s) 988
BsiSI CCGG 5 cut(s) 260, 308, 686, 887, 1115
BsiWI CGTACG 1 cut(s) 711
BslFI GGGAC 2 cut(s) 572, 1081
BslI CCNNNNNNNGG 4 cut(s) 37, 68, 699, 1107
BsmAI GTCTC 4 cut(s) 66, 139, 244, 884
BsmBI CGTCTC 3 cut(s) 66, 139, 244
BsmFI GGGAC 2 cut(s) 572, 1081
BsmI GAATGC 1 cut(s) 1080
BsnI GGCC 5 cut(s) 263, 471, 524, 623, 709
BsoBI CYCGRG 1 cut(s) 988
Bsp1286I GDGCHC 2 cut(s) 662, 1147
Bsp13I TCCGGA 1 cut(s) 685
Bsp143I GATC 4 cut(s) 682, 715, 873, 999
BspANI GGCC 5 cut(s) 263, 471, 524, 623, 709
BspCNI CTCAG 1 cut(s) 226
BspEI TCCGGA 1 cut(s) 685
BspFNI CGCG 4 cut(s) 153, 477, 497, 626
BspLI GGNNCC 6 cut(s) 149, 227, 413, 982, 1069, 1070
BspMI ACCTGC 1 cut(s) 1020
BspPI GGATC 2 cut(s) 677, 994
BsrBI CCGCTC 1 cut(s) 64
BssECI CCNNGG 5 cut(s) 36, 194, 462, 525, 624
BssMI GATC 4 cut(s) 682, 715, 873, 999
BssNI GRCGYC 3 cut(s) 75, 602, 1053
BssSI CACGAG 1 cut(s) 434
BssT1I CCWWGG 1 cut(s) 36
Bst2BI CACGAG 1 cut(s) 434
Bst2UI CCWGG 2 cut(s) 196, 404
Bst4CI ACNGT 3 cut(s) 736, 926, 980
Bst6I CTCTTC 2 cut(s) 354, 601
BstACI GRCGYC 3 cut(s) 75, 602, 1053
BstBAI YACGTR 7 cut(s) 248, 271, 555, 564, 609, 646, 672
BstC8I GCNNGC 5 cut(s) 490, 499, 522, 579, 1010
BstDEI CTNAG 2 cut(s) 21, 213
BstDSI CCRYGG 3 cut(s) 462, 525, 624
BstF5I GGATG 3 cut(s) 108, 301, 1089
BstFNI CGCG 4 cut(s) 153, 477, 497, 626
BstHHI GCGC 2 cut(s) 155, 497
BstKTI GATC 4 cut(s) 685, 718, 876, 1002
BstMAI GTCTC 4 cut(s) 66, 139, 244, 884
BstMBI GATC 4 cut(s) 682, 715, 873, 999
BstMWI GCNNNNNNNGC 4 cut(s) 11, 494, 498, 1120
BstNI CCWGG 2 cut(s) 196, 404
BstNSI RCATGY 2 cut(s) 361, 886
BstSCI CCNGG 3 cut(s) 194, 258, 402
BstUI CGCG 4 cut(s) 153, 477, 497, 626
BstV1I GCAGC 1 cut(s) 943
BstV2I GAAGAC 2 cut(s) 287, 519
BstXI CCANNNNNNTGG 1 cut(s) 438
BsuRI GGCC 5 cut(s) 263, 471, 524, 623, 709
BtgI CCRYGG 3 cut(s) 462, 525, 624
BtrI CACGTC 3 cut(s) 133, 808, 1095
BtsCI GGATG 3 cut(s) 108, 301, 1089
BtsIMutI CAGTG 2 cut(s) 922, 1145
BveI ACCTGC 1 cut(s) 1020
Cac8I GCNNGC 5 cut(s) 490, 499, 522, 579, 1010
CfoI GCGC 2 cut(s) 155, 497
Cfr13I GGNCC 3 cut(s) 469, 707, 1068
Cfr42I CCGCGG 1 cut(s) 627
CseI GACGC 4 cut(s) 401, 591, 710, 1061
Csp6I GTAC 5 cut(s) 535, 643, 673, 712, 1133
CviAII CATG 4 cut(s) 358, 574, 883, 1057
CviQI GTAC 5 cut(s) 535, 643, 673, 712, 1133
DdeI CTNAG 2 cut(s) 21, 213
DpnI GATC 4 cut(s) 684, 717, 875, 1001
DpnII GATC 4 cut(s) 682, 715, 873, 999
DrdI GACNNNNNNGTC 1 cut(s) 455
DriI GACNNNNNGTC 1 cut(s) 850
DseDI GACNNNNNNGTC 1 cut(s) 455
EaeI YGGCCR 2 cut(s) 522, 621
Eam1104I CTCTTC 2 cut(s) 354, 601
Eam1105I GACNNNNNGTC 1 cut(s) 850
EarI CTCTTC 2 cut(s) 354, 601
EciI GGCGGA 1 cut(s) 815
Eco130I CCWWGG 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 1068
Eco72I CACGTG 5 cut(s) 248, 271, 555, 564, 609
Eco88I CYCGRG 1 cut(s) 988
EcoO109I RGGNCCY 1 cut(s) 1068
EcoRII CCWGG 2 cut(s) 194, 402
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
Esp3I CGTCTC 3 cut(s) 66, 139, 244
FaeI CATG 4 cut(s) 361, 577, 886, 1060
FaiI YATR 9 cut(s) 355, 359, 575, 884, 951, 953, 1043, 1058, 1159
FaqI GGGAC 2 cut(s) 572, 1081
FatI CATG 4 cut(s) 357, 573, 882, 1056
FauI CCCGC 3 cut(s) 525, 586, 775
FblI GTMKAC 2 cut(s) 164, 279
Fnu4HI GCNGC 4 cut(s) 42, 493, 624, 932
FokI GGATG 3 cut(s) 95, 288, 1096
Fsp4HI GCNGC 4 cut(s) 42, 493, 624, 932
GlaI GCGC 2 cut(s) 154, 496
GluI GCNGC 4 cut(s) 42, 493, 624, 932
GsuI CTGGAG 1 cut(s) 425
HaeIII GGCC 5 cut(s) 263, 471, 524, 623, 709
HapII CCGG 5 cut(s) 260, 308, 686, 887, 1115
HgaI GACGC 4 cut(s) 401, 591, 710, 1061
HhaI GCGC 2 cut(s) 155, 497
Hin1I GRCGYC 3 cut(s) 75, 602, 1053
Hin1II CATG 4 cut(s) 361, 577, 886, 1060
Hin6I GCGC 2 cut(s) 153, 495
HinP1I GCGC 2 cut(s) 153, 495
HincII GTYRAC 2 cut(s) 280, 763
HindII GTYRAC 2 cut(s) 280, 763
HinfI GANTC 9 cut(s) 83, 292, 346, 569, 593, 689, 727, 756, 851
HpaII CCGG 5 cut(s) 260, 308, 686, 887, 1115
HphI GGTGA 2 cut(s) 890, 1102
Hpy166II GTNNAC 6 cut(s) 165, 208, 280, 606, 763, 1035
Hpy188I TCNGA 5 cut(s) 216, 726, 732, 985, 1142
Hpy188III TCNNGA 1 cut(s) 686
Hpy8I GTNNAC 6 cut(s) 165, 208, 280, 606, 763, 1035
Hpy99I CGWCG 9 cut(s) 206, 239, 284, 395, 488, 736, 848, 851, 1067
HpyAV CCTTC 3 cut(s) 378, 805, 904
HpyCH4III ACNGT 3 cut(s) 736, 926, 980
HpyCH4V TGCA 3 cut(s) 1029, 1078, 1123
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 494, 498, 1120
HpyF3I CTNAG 2 cut(s) 21, 213
Hsp92I GRCGYC 3 cut(s) 75, 602, 1053
Hsp92II CATG 4 cut(s) 361, 577, 886, 1060
HspAI GCGC 2 cut(s) 153, 495
KflI GGGWCCC 1 cut(s) 1068
Kpn2I TCCGGA 1 cut(s) 685
KspI CCGCGG 1 cut(s) 627
Kzo9I GATC 4 cut(s) 682, 715, 873, 999
LmnI GCTCC 4 cut(s) 338, 411, 749, 1142
Lsp1109I GCAGC 1 cut(s) 943
MaeIII GTNAC 6 cut(s) 7, 128, 646, 667, 878, 1090
MalI GATC 4 cut(s) 684, 717, 875, 1001
MbiI CCGCTC 1 cut(s) 64
MboI GATC 4 cut(s) 682, 715, 873, 999
MboII GAAGA 6 cut(s) 62, 65, 287, 341, 524, 588
MhlI GDGCHC 2 cut(s) 662, 1147
MluCI AATT 3 cut(s) 98, 374, 1152
MlyI GAGTC 7 cut(s) 92, 301, 340, 578, 587, 698, 860
MmeI TCCRAC 4 cut(s) 755, 837, 1008, 1120
MroI TCCGGA 1 cut(s) 685
MseI TTAA 1 cut(s) 189
MslI CAYNNNNRTG 1 cut(s) 436
MspA1I CMGCKG 2 cut(s) 520, 626
MspI CCGG 5 cut(s) 260, 308, 686, 887, 1115
MspR9I CCNGG 3 cut(s) 196, 260, 404
Mva1269I GAATGC 1 cut(s) 1080
MvaI CCWGG 2 cut(s) 196, 404
MvnI CGCG 4 cut(s) 153, 477, 497, 626
MwoI GCNNNNNNNGC 4 cut(s) 11, 494, 498, 1120
NciI CCSGG 1 cut(s) 260
NdeII GATC 4 cut(s) 682, 715, 873, 999
NlaIII CATG 4 cut(s) 361, 577, 886, 1060
NlaIV GGNNCC 6 cut(s) 149, 227, 413, 982, 1069, 1070
NmeAIII GCCGAG 1 cut(s) 641
NmuCI GTSAC 5 cut(s) 128, 646, 667, 878, 1090
NspI RCATGY 2 cut(s) 361, 886
OliI CACNNNNGTG 1 cut(s) 436
PaqCI CACCTGC 1 cut(s) 1020
PcsI WCGNNNNNNNCGW 5 cut(s) 276, 560, 605, 668, 1059
PctI GAATGC 1 cut(s) 1080
PfeI GAWTC 2 cut(s) 727, 756
Pfl23II CGTACG 1 cut(s) 711
PflMI CCANNNNNTGG 1 cut(s) 699
PkrI GCNGC 4 cut(s) 43, 494, 625, 933
PleI GAGTC 7 cut(s) 91, 300, 340, 577, 587, 697, 859
PmaCI CACGTG 5 cut(s) 248, 271, 555, 564, 609
PmlI CACGTG 5 cut(s) 248, 271, 555, 564, 609
PpsI GAGTC 7 cut(s) 91, 300, 340, 577, 587, 697, 859
Ppu21I YACGTR 7 cut(s) 248, 271, 555, 564, 609, 646, 672
PpuMI RGGWCCY 1 cut(s) 1068
Psp5II RGGWCCY 1 cut(s) 1068
Psp6I CCWGG 2 cut(s) 194, 402
PspCI CACGTG 5 cut(s) 248, 271, 555, 564, 609
PspGI CCWGG 2 cut(s) 194, 402
PspLI CGTACG 1 cut(s) 711
PspN4I GGNNCC 6 cut(s) 149, 227, 413, 982, 1069, 1070
PspPI GGNCC 3 cut(s) 469, 707, 1068
PspPPI RGGWCCY 1 cut(s) 1068
RsaI GTAC 5 cut(s) 536, 644, 674, 713, 1134
RsaNI GTAC 5 cut(s) 535, 643, 673, 712, 1133
RseI CAYNNNNRTG 1 cut(s) 436
SacII CCGCGG 1 cut(s) 627
SalI GTCGAC 1 cut(s) 278
SaqAI TTAA 1 cut(s) 189
SatI GCNGC 4 cut(s) 42, 493, 624, 932
Sau3AI GATC 4 cut(s) 682, 715, 873, 999
Sau96I GGNCC 3 cut(s) 469, 707, 1068
SchI GAGTC 7 cut(s) 92, 301, 340, 578, 587, 698, 860
ScrFI CCNGG 3 cut(s) 196, 260, 404
SduI GDGCHC 2 cut(s) 662, 1147
Sfr303I CCGCGG 1 cut(s) 627
SgrBI CCGCGG 1 cut(s) 627
SinI GGWCC 1 cut(s) 1068
SmiMI CAYNNNNRTG 1 cut(s) 436
SmlI CTYRAG 1 cut(s) 1013
SmoI CTYRAG 1 cut(s) 1013
Sse9I AATT 3 cut(s) 98, 374, 1152
StyD4I CCNGG 3 cut(s) 194, 258, 402
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 3 cut(s) 736, 926, 980
TaqI TCGA 5 cut(s) 255, 279, 344, 390, 941
TasI AATT 3 cut(s) 98, 374, 1152
TauI GCSGC 3 cut(s) 44, 495, 626
TfiI GAWTC 2 cut(s) 727, 756
Tru1I TTAA 1 cut(s) 189
Tru9I TTAA 1 cut(s) 189
TscAI CASTG 2 cut(s) 929, 1152
TseFI GTSAC 5 cut(s) 128, 646, 667, 878, 1090
TseI GCWGC 1 cut(s) 931
Tsp45I GTSAC 5 cut(s) 128, 646, 667, 878, 1090
TspDTI ATGAA 2 cut(s) 287, 1073
TspGWI ACGGA 6 cut(s) 76, 84, 161, 304, 665, 736
TspRI CASTG 2 cut(s) 929, 1152
Van91I CCANNNNNTGG 1 cut(s) 699
VpaK11BI GGWCC 1 cut(s) 1068
XapI RAATTY 2 cut(s) 374, 1152
XceI RCATGY 2 cut(s) 361, 886
XmiI GTMKAC 2 cut(s) 164, 279
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.