pycom16g08250

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010437
Physical Location & Seq
Forward (+)
5443547 .. 5444698
1152 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g08250.1

Sequence Viewer

Length: 1152 bp
ATGGCGGCTGAGGAGCTTGAAGACCAAGCTCGGACAGAAACTTCCCACACCGAAGTCCGGCAAGGACCTCACGAACTCGACGGAGACATCTTAGAGTCCATACTCTCCCGCGTGCCTCTCCTCCACCTCGTGCCTGCCTGCCATGTGTCCAAGTCCTGGAACCGCACCGTCTCTTCCTCTCTCCGCCGCTTCCACCGAAACGTCAAGCCCTGGCTCATTGTCCTCGCTCAAGCCACCAGGTCTCCCTACCACACCACCGCACGTGCCTACGACCCCAACTCGCACGCTTGGATCGACATCCACTCTCAGCCGATGATCGGATCCATCTCCACCCTCCGATCCTCGAACTCCCTCCTCTACGCGCTTTCTCCCTCTAAATTCGCCTTTTCAACTGATCCACTGCACTACACGTGGCACACGGTGGATGCCCCGCTGATATGGCGCACGGACCCGATTACCGGCGTGGTGGGCCACCGCATCGTCGTCGCTGGCGGCACGTGTGATTTCGAGGACGATCCACTTTCGGTGGAAATGTACGACACTGTGTCAAAAACGTGGACCATGTGCGACTCGATACCCGCTGTTCTCGAGGACTCTGCAGCCTCCACCTGGCTCTCTGTCGCCGTCGACGACAGCAAAATGTACGTGATGGATAAAGGCTCCGGTGTGACATGCTCGTTCGACCCGGATTCCAGCACGTGGTCTGGACCGTATGATCTGCGTCCCGATGAGACGGTTTTTAGCGCCGTCATTGGATTCGCTGACGACCGTTTGATCTTAGTGGGCGCGGTGGGAAGCGCAGAGAATTTGGAAGGCGTGAAAGTGTGGGAAGTGAACGGCGTGTCGTTTGAGTGTAAAGAGTTGGTCGGGGAGATGCCAGAGGCAATGGTGGAGAAGCTGAAGGGCGAGAGCGATCGCGTGACGTCGATTGGGATGAGCTGCATTGGACATATGGTGTGCCTGCATAACCCTTGGTCGCCGGAGGAGCTGATCCTCTGCGAGCTTGATGAGGGTGGGTGCAGCTGGGGTAGCGTACGGAATGCGGCGGTGGACGACAGGACTCGAATGCAGAAGCTGGTGGTGACGTGTTCGAAAGTAGGTTTGCCGGATTTGCATAAAGCCTTACAAGTTGGAGCGCTGAGATTGGTTTGA

Protein Analysis

384

Amino Acids

42.11

Weight (kDa)

5.04

Isoelectric Point (pI)

43.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 91 - 236 6.1e-09 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 926
AccB7I CCANNNNNTGG 2 cut(s) 156, 699
AccI GTMKAC 1 cut(s) 627
AccII CGCG 4 cut(s) 111, 362, 788, 918
AclWI GGATC 7 cut(s) 299, 315, 328, 333, 389, 509, 985
AcsI RAATTY 2 cut(s) 377, 805
AcuI CTGAAG 1 cut(s) 920
AcvI CACGTG 4 cut(s) 263, 411, 498, 699
AcyI GRCGYC 1 cut(s) 923
AdeI CACNNNGTG 2 cut(s) 130, 421
AfaI GTAC 3 cut(s) 536, 644, 1035
AfeI AGCGCT 1 cut(s) 1137
AfiI CCNNNNNNNGG 6 cut(s) 57, 156, 317, 458, 609, 699
AflIII ACRYGT 3 cut(s) 408, 497, 1085
AgsI TTSAA 2 cut(s) 20, 390
AhdI GACNNNNNGTC 1 cut(s) 544
AjiI CACGTC 1 cut(s) 1086
AjnI CCWGG 4 cut(s) 155, 209, 236, 608
AluBI AGCT 8 cut(s) 16, 29, 898, 939, 988, 1003, 1023, 1075
AluI AGCT 8 cut(s) 16, 29, 898, 939, 988, 1003, 1023, 1075
Alw26I GTCTC 4 cut(s) 78, 175, 246, 725
AlwI GGATC 7 cut(s) 299, 315, 328, 333, 389, 509, 985
AlwNI CAGNNNCTG 1 cut(s) 1075
Ama87I CYCGRG 1 cut(s) 587
Aor51HI AGCGCT 1 cut(s) 1137
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 3 cut(s) 599, 939, 1020
ApoI RAATTY 2 cut(s) 377, 805
AsiSI GCGATCGC 1 cut(s) 916
AspLEI GCGC 6 cut(s) 364, 444, 746, 788, 800, 1138
AspS9I GGNCC 5 cut(s) 65, 448, 469, 558, 707
AsuC2I CCSGG 1 cut(s) 686
AsuHPI GGTGA 1 cut(s) 1093
AsuII TTCGAA 1 cut(s) 1091
AvaI CYCGRG 1 cut(s) 587
AvaII GGWCC 4 cut(s) 65, 448, 558, 707
BamHI GGATCC 1 cut(s) 320
BauI CACGAG 1 cut(s) 128
BbrPI CACGTG 4 cut(s) 263, 411, 498, 699
BbsI GAAGAC 1 cut(s) 27
BbvCI CCTCAGC 1 cut(s) 9
BbvI GCAGC 3 cut(s) 611, 926, 1032
BccI CCATC 2 cut(s) 332, 643
BceAI ACGGC 3 cut(s) 608, 731, 853
BcgI CGANNNNNNTGC 4 cut(s) 466, 500, 578, 612
BciT130I CCWGG 4 cut(s) 157, 211, 238, 610
BcnI CCSGG 1 cut(s) 686
BcoDI GTCTC 4 cut(s) 78, 175, 246, 725
BfmI CTRYAG 1 cut(s) 597
BfoI RGCGCY 2 cut(s) 747, 1139
BisI GCNGC 7 cut(s) 6, 187, 493, 600, 940, 1021, 1044
BlsI GCNGC 7 cut(s) 7, 188, 494, 601, 941, 1022, 1045
Bme1390I CCNGG 5 cut(s) 157, 211, 238, 610, 686
Bme18I GGWCC 4 cut(s) 65, 448, 558, 707
BmeRI GACNNNNNGTC 1 cut(s) 544
BmeT110I CYCGRG 1 cut(s) 587
BmgBI CACGTC 1 cut(s) 1086
BmgT120I GGNCC 5 cut(s) 65, 448, 469, 558, 707
BmiI GGNNCC 4 cut(s) 161, 322, 450, 661
BmrFI CCNGG 5 cut(s) 157, 211, 238, 610, 686
BmsI GCATC 3 cut(s) 415, 486, 864
BpiI GAAGAC 1 cut(s) 27
Bpu10I CCTNAGC 1 cut(s) 9
Bpu14I TTCGAA 1 cut(s) 1091
BpuEI CTTGAG 1 cut(s) 213
BpuMI CCSGG 1 cut(s) 686
BsaAI YACGTR 5 cut(s) 263, 411, 498, 646, 699
BsaBI GATNNNNATC 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 923
BsaI GGTCTC 1 cut(s) 246
BsaJI CCNNGG 2 cut(s) 209, 971
BsaWI WCCGGW 1 cut(s) 662
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bsc4I CCNNNNNNNGG 6 cut(s) 57, 156, 317, 458, 609, 699
Bse118I RCCGGY 1 cut(s) 458
Bse3DI GCAATG 1 cut(s) 891
Bse8I GATNNNNATC 1 cut(s) 296
BseBI CCWGG 4 cut(s) 157, 211, 238, 610
BseDI CCNNGG 2 cut(s) 209, 971
BseGI GGATG 3 cut(s) 297, 430, 939
BseJI GATNNNNATC 1 cut(s) 296
BseLI CCNNNNNNNGG 6 cut(s) 57, 156, 317, 458, 609, 699
BseMI GCAATG 1 cut(s) 891
BseMII CTCAG 2 cut(s) 320, 1130
BseRI GAGGAG 4 cut(s) 26, 110, 344, 998
BseXI GCAGC 3 cut(s) 611, 926, 1032
BseYI CCCAGC 1 cut(s) 1023
BsgI GTGCAG 2 cut(s) 386, 1039
Bsh1236I CGCG 4 cut(s) 111, 362, 788, 918
Bsh1285I CGRYCG 2 cut(s) 769, 916
BshFI GGCC 1 cut(s) 471
BsiEI CGRYCG 2 cut(s) 769, 916
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 6 cut(s) 58, 459, 663, 686, 980, 1106
BsiWI CGTACG 1 cut(s) 1033
BslFI GGGAC 1 cut(s) 708
BslI CCNNNNNNNGG 6 cut(s) 57, 156, 317, 458, 609, 699
BsmAI GTCTC 4 cut(s) 78, 175, 246, 725
BsmBI CGTCTC 2 cut(s) 175, 725
BsmFI GGGAC 1 cut(s) 708
BsmI GAATGC 2 cut(s) 1045, 1071
BsnI GGCC 1 cut(s) 471
Bso31I GGTCTC 1 cut(s) 246
BsoBI CYCGRG 1 cut(s) 587
Bsp119I TTCGAA 1 cut(s) 1091
BspANI GGCC 1 cut(s) 471
BspCNI CTCAG 2 cut(s) 319, 1131
BspFNI CGCG 4 cut(s) 111, 362, 788, 918
BspLI GGNNCC 4 cut(s) 161, 322, 450, 661
BspMAI CTGCAG 1 cut(s) 601
BspPI GGATC 7 cut(s) 299, 315, 328, 333, 389, 509, 985
BspT104I TTCGAA 1 cut(s) 1091
BspTNI GGTCTC 1 cut(s) 246
BsrDI GCAATG 1 cut(s) 891
BsrFI RCCGGY 1 cut(s) 458
BssAI RCCGGY 1 cut(s) 458
BssECI CCNNGG 2 cut(s) 209, 971
BssNI GRCGYC 1 cut(s) 923
BssSI CACGAG 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 971
Bst2BI CACGAG 1 cut(s) 128
Bst2UI CCWGG 4 cut(s) 157, 211, 238, 610
Bst4CI ACNGT 6 cut(s) 169, 421, 544, 711, 736, 770
Bst6I CTCTTC 1 cut(s) 178
BstACI GRCGYC 1 cut(s) 923
BstBAI YACGTR 5 cut(s) 263, 411, 498, 646, 699
BstBI TTCGAA 1 cut(s) 1091
BstC8I GCNNGC 7 cut(s) 113, 135, 139, 285, 490, 962, 1001
BstDEI CTNAG 5 cut(s) 9, 91, 306, 778, 1139
BstF5I GGATG 3 cut(s) 297, 430, 939
BstFNI CGCG 4 cut(s) 111, 362, 788, 918
BstH2I RGCGCY 2 cut(s) 747, 1139
BstHHI GCGC 6 cut(s) 364, 444, 746, 788, 800, 1138
BstMAI GTCTC 4 cut(s) 78, 175, 246, 725
BstMCI CGRYCG 2 cut(s) 769, 916
BstMWI GCNNNNNNNGC 5 cut(s) 439, 468, 985, 1029, 1111
BstNI CCWGG 4 cut(s) 157, 211, 238, 610
BstNSI RCATGY 1 cut(s) 675
BstSCI CCNGG 5 cut(s) 155, 209, 236, 608, 684
BstSFI CTRYAG 1 cut(s) 597
BstUI CGCG 4 cut(s) 111, 362, 788, 918
BstV1I GCAGC 3 cut(s) 611, 926, 1032
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 1 cut(s) 320
BstYI RGATCY 1 cut(s) 320
BsuRI GGCC 1 cut(s) 471
BtrI CACGTC 1 cut(s) 1086
BtsCI GGATG 3 cut(s) 297, 430, 939
BtsI GCAGTG 1 cut(s) 398
BtsIMutI CAGTG 2 cut(s) 398, 540
Cac8I GCNNGC 7 cut(s) 113, 135, 139, 285, 490, 962, 1001
CaiI CAGNNNCTG 1 cut(s) 1075
CfoI GCGC 6 cut(s) 364, 444, 746, 788, 800, 1138
Cfr10I RCCGGY 1 cut(s) 458
Cfr13I GGNCC 5 cut(s) 65, 448, 469, 558, 707
CseI GACGC 1 cut(s) 710
CsiI ACCWGGT 1 cut(s) 236
Csp6I GTAC 3 cut(s) 535, 643, 1034
CviAII CATG 3 cut(s) 143, 562, 672
CviQI GTAC 3 cut(s) 535, 643, 1034
DdeI CTNAG 5 cut(s) 9, 91, 306, 778, 1139
DraIII CACNNNGTG 2 cut(s) 130, 421
DriI GACNNNNNGTC 1 cut(s) 544
Eam1104I CTCTTC 1 cut(s) 178
Eam1105I GACNNNNNGTC 1 cut(s) 544
EarI CTCTTC 1 cut(s) 178
EciI GGCGGA 1 cut(s) 173
Eco130I CCWWGG 1 cut(s) 971
Eco31I GGTCTC 1 cut(s) 246
Eco47I GGWCC 4 cut(s) 65, 448, 558, 707
Eco47III AGCGCT 1 cut(s) 1137
Eco57I CTGAAG 1 cut(s) 920
Eco72I CACGTG 4 cut(s) 263, 411, 498, 699
Eco88I CYCGRG 1 cut(s) 587
EcoO109I RGGNCCY 1 cut(s) 65
EcoRII CCWGG 4 cut(s) 155, 209, 236, 608
EcoT14I CCWWGG 1 cut(s) 971
ErhI CCWWGG 1 cut(s) 971
Esp3I CGTCTC 2 cut(s) 175, 725
FaeI CATG 3 cut(s) 146, 565, 675
FaqI GGGAC 1 cut(s) 708
FatI CATG 3 cut(s) 142, 561, 671
FauI CCCGC 3 cut(s) 116, 438, 586
FauNDI CATATG 1 cut(s) 951
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 7 cut(s) 6, 187, 493, 600, 940, 1021, 1044
FokI GGATG 3 cut(s) 284, 437, 946
Fsp4HI GCNGC 7 cut(s) 6, 187, 493, 600, 940, 1021, 1044
GlaI GCGC 6 cut(s) 363, 443, 745, 787, 799, 1137
GluI GCNGC 7 cut(s) 6, 187, 493, 600, 940, 1021, 1044
GsaI CCCAGC 1 cut(s) 1027
HaeII RGCGCY 2 cut(s) 747, 1139
HaeIII GGCC 1 cut(s) 471
HapII CCGG 6 cut(s) 58, 459, 663, 686, 980, 1106
HgaI GACGC 1 cut(s) 710
HhaI GCGC 6 cut(s) 364, 444, 746, 788, 800, 1138
Hin1I GRCGYC 1 cut(s) 923
Hin1II CATG 3 cut(s) 146, 565, 675
Hin6I GCGC 6 cut(s) 362, 442, 744, 786, 798, 1136
HinP1I GCGC 6 cut(s) 362, 442, 744, 786, 798, 1136
HincII GTYRAC 1 cut(s) 628
HindII GTYRAC 1 cut(s) 628
HinfI GANTC 6 cut(s) 95, 569, 593, 689, 756, 1060
HpaII CCGG 6 cut(s) 58, 459, 663, 686, 980, 1106
HphI GGTGA 1 cut(s) 1093
Hpy166II GTNNAC 4 cut(s) 558, 628, 835, 1051
Hpy188I TCNGA 3 cut(s) 33, 320, 338
Hpy188III TCNNGA 4 cut(s) 71, 587, 705, 725
Hpy8I GTNNAC 4 cut(s) 558, 628, 835, 1051
Hpy99I CGWCG 6 cut(s) 83, 485, 488, 629, 632, 928
HpyAV CCTTC 2 cut(s) 806, 895
HpyCH4III ACNGT 6 cut(s) 169, 421, 544, 711, 736, 770
HpyCH4IV ACGT 9 cut(s) 201, 262, 410, 497, 554, 645, 698, 923, 1085
HpyCH4V TGCA 7 cut(s) 403, 599, 942, 964, 1020, 1069, 1114
HpyF10VI GCNNNNNNNGC 5 cut(s) 439, 468, 985, 1029, 1111
HpyF3I CTNAG 5 cut(s) 9, 91, 306, 778, 1139
HpySE526I ACGT 9 cut(s) 201, 262, 410, 497, 554, 645, 698, 923, 1085
Hsp92I GRCGYC 1 cut(s) 923
Hsp92II CATG 3 cut(s) 146, 565, 675
HspAI GCGC 6 cut(s) 362, 442, 744, 786, 798, 1136
LmnI GCTCC 4 cut(s) 13, 665, 985, 1133
Lsp1109I GCAGC 3 cut(s) 611, 926, 1032
LweI GCATC 3 cut(s) 415, 486, 864
MabI ACCWGGT 1 cut(s) 236
MaeII ACGT 9 cut(s) 201, 262, 410, 497, 554, 645, 698, 923, 1085
MaeIII GTNAC 3 cut(s) 667, 919, 1081
MboII GAAGA 2 cut(s) 32, 165
MflI RGATCY 1 cut(s) 320
MluCI AATT 2 cut(s) 377, 805
MlyI GAGTC 4 cut(s) 104, 563, 587, 1054
MmeI TCCRAC 1 cut(s) 1111
MspA1I CMGCKG 3 cut(s) 433, 581, 1023
MspI CCGG 6 cut(s) 58, 459, 663, 686, 980, 1106
MspR9I CCNGG 5 cut(s) 157, 211, 238, 610, 686
Mva1269I GAATGC 2 cut(s) 1045, 1071
MvaI CCWGG 4 cut(s) 157, 211, 238, 610
MvnI CGCG 4 cut(s) 111, 362, 788, 918
MwoI GCNNNNNNNGC 5 cut(s) 439, 468, 985, 1029, 1111
NciI CCSGG 1 cut(s) 686
NdeI CATATG 1 cut(s) 951
NlaIII CATG 3 cut(s) 146, 565, 675
NlaIV GGNNCC 4 cut(s) 161, 322, 450, 661
NmuCI GTSAC 3 cut(s) 667, 919, 1081
NspI RCATGY 1 cut(s) 675
NspV TTCGAA 1 cut(s) 1091
PaeR7I CTCGAG 1 cut(s) 587
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 627
PctI GAATGC 2 cut(s) 1045, 1071
PfeI GAWTC 2 cut(s) 689, 756
Pfl23II CGTACG 1 cut(s) 1033
PflMI CCANNNNNTGG 2 cut(s) 156, 699
PfoI TCCNGGA 1 cut(s) 155
PkrI GCNGC 7 cut(s) 7, 188, 494, 601, 941, 1022, 1045
Ple19I CGATCG 1 cut(s) 916
PleI GAGTC 4 cut(s) 103, 563, 587, 1054
PmaCI CACGTG 4 cut(s) 263, 411, 498, 699
PmlI CACGTG 4 cut(s) 263, 411, 498, 699
PpsI GAGTC 4 cut(s) 103, 563, 587, 1054
Ppu21I YACGTR 5 cut(s) 263, 411, 498, 646, 699
PpuMI RGGWCCY 1 cut(s) 65
Psp5II RGGWCCY 1 cut(s) 65
Psp6I CCWGG 4 cut(s) 155, 209, 236, 608
PspCI CACGTG 4 cut(s) 263, 411, 498, 699
PspFI CCCAGC 1 cut(s) 1023
PspGI CCWGG 4 cut(s) 155, 209, 236, 608
PspLI CGTACG 1 cut(s) 1033
PspN4I GGNNCC 4 cut(s) 161, 322, 450, 661
PspPI GGNCC 5 cut(s) 65, 448, 469, 558, 707
PspPPI RGGWCCY 1 cut(s) 65
PstI CTGCAG 1 cut(s) 601
PstNI CAGNNNCTG 1 cut(s) 1075
PsuI RGATCY 1 cut(s) 320
PvuI CGATCG 1 cut(s) 916
PvuII CAGCTG 1 cut(s) 1023
RgaI GCGATCGC 1 cut(s) 916
RsaI GTAC 3 cut(s) 536, 644, 1035
RsaNI GTAC 3 cut(s) 535, 643, 1034
SalI GTCGAC 1 cut(s) 626
SatI GCNGC 7 cut(s) 6, 187, 493, 600, 940, 1021, 1044
Sau96I GGNCC 5 cut(s) 65, 448, 469, 558, 707
SchI GAGTC 4 cut(s) 104, 563, 587, 1054
ScrFI CCNGG 5 cut(s) 157, 211, 238, 610, 686
SexAI ACCWGGT 1 cut(s) 236
SfaAI GCGATCGC 1 cut(s) 916
SfaNI GCATC 3 cut(s) 415, 486, 864
SfcI CTRYAG 1 cut(s) 597
Sfr274I CTCGAG 1 cut(s) 587
SfuI TTCGAA 1 cut(s) 1091
SgfI GCGATCGC 1 cut(s) 916
SgrDI CGTCGACG 1 cut(s) 626
SinI GGWCC 4 cut(s) 65, 448, 558, 707
SlaI CTCGAG 1 cut(s) 587
SmlI CTYRAG 2 cut(s) 228, 587
SmoI CTYRAG 2 cut(s) 228, 587
Sse9I AATT 2 cut(s) 377, 805
StyD4I CCNGG 5 cut(s) 155, 209, 236, 608, 684
StyI CCWWGG 1 cut(s) 971
TaaI ACNGT 6 cut(s) 169, 421, 544, 711, 736, 770
TaiI ACGT 9 cut(s) 204, 265, 413, 500, 557, 648, 701, 926, 1088
TasI AATT 2 cut(s) 377, 805
TauI GCSGC 4 cut(s) 8, 189, 495, 1046
TfiI GAWTC 2 cut(s) 689, 756
TscAI CASTG 2 cut(s) 405, 547
TseFI GTSAC 3 cut(s) 667, 919, 1081
TseI GCWGC 3 cut(s) 599, 939, 1020
Tsp45I GTSAC 3 cut(s) 667, 919, 1081
TspGWI ACGGA 3 cut(s) 96, 461, 1051
TspRI CASTG 2 cut(s) 405, 547
Van91I CCANNNNNTGG 2 cut(s) 156, 699
VpaK11BI GGWCC 4 cut(s) 65, 448, 558, 707
XapI RAATTY 2 cut(s) 377, 805
XceI RCATGY 1 cut(s) 675
XhoI CTCGAG 1 cut(s) 587
XmiI GTMKAC 1 cut(s) 627
ZraI GACGTC 1 cut(s) 924
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.