RchiOBHm_Chr4g0441991

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0010437
Physical Location & Seq
Forward (+)
63726035 .. 63727443
1409 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40978

Sequence Viewer

Length: 1161 bp
ATGGCAGTAACGGCGAACCCTAAGCAGAACCAGACCAAGGCGGCGGTTGAAGAAGAAGCACCGCTCTACGGAGACGTCTTGGAGTCCGTGCTATCTAAATTACACCTCATCCATCTCCTATCGGCGTGTCACGTCTCAAAGTCTTGGAACCGCGCCGTTTCGTCTACCCTCCGTTTCTCTAACCGAGTTAAACCCTGGCTCGTCGTCCACACTCAGACCACCAGGTTCCCCTACGTCGTCTCCGCACGTGCTTACGACCCGGCCTCGCACGTGTGGGTCGACCTAGAACGGAGTCTTCATCATCCTCCGGTCAAGTCCATCTCCACCCTCCGCTCCTCTCACTCGACTCTTCTCTACATGCTTTCCCCTTCCAAATTCGCTTTCTCCCTCGACGCGCTCCACCTAGAGTGGAGTCACGCAAATACGCCTCCGCTCGTGTGGCGAACTGACCCGATTGTCGCCGTGGTGGGCCACCGCGTAGTCGTCGCAGGAGGCGCGTGCGACTACGAGGACGACCCGCTGGCCGTGGAAATGTACGATGTCAAAGTTGGCACGTGGGACACGTGCGAGTCCATGCCCGCCATATTCAAAGACTCTTCGGCGTCCACGTGGCTGTCAGTGGCCGCGGACGAGAGGAGAATGTACGTGACGGAGAGGTGCTCGGCTGTCACGTACTCGTTTGATCCGGAGTCCAAGAAGTGGTTTGGGCCGTACGATCTGCGTCCGAATCCGACGGTGTTTAGCTCCGTGATTGGATTCGTTAACGGGCGTTTGGTTTTGGCGGGAGTTGTGGGAGCGGCGGAGGACGTGAAGGGAGTGAAGTTGTGGGAAGTGGAAGTCAGCGACGACGAGTCGTTGGAGTTGAAAGAGATGATCGGTGACATGCCGGAGACAATGGTGGAGAAGTTGAAGGGCGAGAGAGACTGTGAGCCGTCGTTTTCGATGAGCTATATGGGAGGTATGGTGTGTTTTCACAACGGTTCCGACCCAAGCGAGTTGATCCTCTGCGAGCTGGATAATGGGGGGTGCAGGTGGACGAGCATGAGGAATGATGCCGTGAACGACGGGACCCGCATGCAGAGGATGGTGGTGACGTGTGCCAATCTGGGGTTGCCGGAGTTGCACAAAGCCGTACAAGTCGGAGCACTGAAAATTCTATGA

Protein Analysis

386

Amino Acids

42.81

Weight (kDa)

5.64

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 122 - 234 9.8e-07 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1020
AasI GACNNNNNNGTC 1 cut(s) 455
AatII GACGTC 1 cut(s) 78
Acc36I ACCTGC 1 cut(s) 1020
AccB7I CCANNNNNTGG 1 cut(s) 699
AccBSI CCGCTC 4 cut(s) 64, 333, 433, 797
AccI GTMKAC 2 cut(s) 164, 279
AccII CGCG 5 cut(s) 153, 395, 477, 497, 626
AccIII TCCGGA 1 cut(s) 685
AclWI GGATC 2 cut(s) 677, 994
AcoI YGGCCR 2 cut(s) 522, 621
AcsI RAATTY 2 cut(s) 374, 1152
AcvI CACGTG 5 cut(s) 248, 271, 555, 564, 609
AcyI GRCGYC 2 cut(s) 75, 602
AfaI GTAC 5 cut(s) 536, 644, 674, 713, 1134
AfiI CCNNNNNNNGG 4 cut(s) 37, 68, 699, 1107
AflIII ACRYGT 3 cut(s) 270, 561, 1094
AgsI TTSAA 4 cut(s) 50, 589, 865, 910
AhdI GACNNNNNGTC 1 cut(s) 850
AjiI CACGTC 3 cut(s) 133, 808, 1095
AjnI CCWGG 2 cut(s) 194, 221
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 3 cut(s) 744, 948, 1012
AluI AGCT 3 cut(s) 744, 948, 1012
Alw21I GWGCWC 2 cut(s) 662, 1147
Alw26I GTCTC 5 cut(s) 66, 139, 244, 884, 915
AlwI GGATC 2 cut(s) 677, 994
Aor13HI TCCGGA 1 cut(s) 685
AoxI GGCC 5 cut(s) 261, 469, 522, 621, 707
ApoI RAATTY 2 cut(s) 374, 1152
AspLEI GCGC 3 cut(s) 155, 397, 497
AspS9I GGNCC 3 cut(s) 469, 707, 1068
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 2 cut(s) 890, 1102
AvaII GGWCC 1 cut(s) 1068
BauI CACGAG 1 cut(s) 434
BbrPI CACGTG 5 cut(s) 248, 271, 555, 564, 609
BbsI GAAGAC 1 cut(s) 287
Bbv12I GWGCWC 2 cut(s) 662, 1147
BccI CCATC 3 cut(s) 120, 326, 1078
BceAI ACGGC 8 cut(s) 27, 140, 446, 509, 694, 916, 1040, 1115
BciT130I CCWGG 2 cut(s) 196, 223
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 5 cut(s) 66, 139, 244, 884, 915
BfaI CTAG 2 cut(s) 284, 404
BfuAI ACCTGC 1 cut(s) 1020
BisI GCNGC 3 cut(s) 42, 624, 798
BlsI GCNGC 3 cut(s) 43, 625, 799
Bme1390I CCNGG 3 cut(s) 196, 223, 260
Bme18I GGWCC 1 cut(s) 1068
BmeRI GACNNNNNGTC 1 cut(s) 850
BmgBI CACGTC 3 cut(s) 133, 808, 1095
BmgT120I GGNCC 3 cut(s) 469, 707, 1068
BmiI GGNNCC 5 cut(s) 149, 227, 982, 1069, 1070
BmrFI CCNGG 3 cut(s) 196, 223, 260
BmsI GCATC 1 cut(s) 1042
BpiI GAAGAC 1 cut(s) 287
BplI GAGNNNNNCTC 2 cut(s) 644, 676
Bpu10I CCTNAGC 1 cut(s) 21
BpuMI CCSGG 1 cut(s) 260
BsaAI YACGTR 7 cut(s) 248, 271, 555, 564, 609, 646, 672
BsaHI GRCGYC 2 cut(s) 75, 602
BsaJI CCNNGG 5 cut(s) 36, 194, 462, 525, 624
BsaWI WCCGGW 2 cut(s) 307, 685
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 948, 978
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 68, 699, 1107
BseAI TCCGGA 1 cut(s) 685
BseBI CCWGG 2 cut(s) 196, 223
BseDI CCNNGG 5 cut(s) 36, 194, 462, 525, 624
BseGI GGATG 3 cut(s) 108, 301, 1089
BseLI CCNNNNNNNGG 4 cut(s) 37, 68, 699, 1107
BseMII CTCAG 1 cut(s) 227
BseRI GAGGAG 2 cut(s) 325, 649
BsgI GTGCAG 1 cut(s) 1048
Bsh1236I CGCG 5 cut(s) 153, 395, 477, 497, 626
BshFI GGCC 5 cut(s) 263, 471, 524, 623, 709
BsiHKAI GWGCWC 2 cut(s) 662, 1147
BsiSI CCGG 5 cut(s) 260, 308, 686, 887, 1115
BsiWI CGTACG 1 cut(s) 711
BslFI GGGAC 2 cut(s) 572, 1081
BslI CCNNNNNNNGG 4 cut(s) 37, 68, 699, 1107
BsmAI GTCTC 5 cut(s) 66, 139, 244, 884, 915
BsmBI CGTCTC 3 cut(s) 66, 139, 244
BsmFI GGGAC 2 cut(s) 572, 1081
BsnI GGCC 5 cut(s) 263, 471, 524, 623, 709
Bsp1286I GDGCHC 2 cut(s) 662, 1147
Bsp13I TCCGGA 1 cut(s) 685
Bsp143I GATC 4 cut(s) 682, 715, 873, 999
BspANI GGCC 5 cut(s) 263, 471, 524, 623, 709
BspCNI CTCAG 1 cut(s) 226
BspEI TCCGGA 1 cut(s) 685
BspFNI CGCG 5 cut(s) 153, 395, 477, 497, 626
BspLI GGNNCC 5 cut(s) 149, 227, 982, 1069, 1070
BspMI ACCTGC 1 cut(s) 1020
BspPI GGATC 2 cut(s) 677, 994
BsrBI CCGCTC 4 cut(s) 64, 333, 433, 797
BssECI CCNNGG 5 cut(s) 36, 194, 462, 525, 624
BssMI GATC 4 cut(s) 682, 715, 873, 999
BssNI GRCGYC 2 cut(s) 75, 602
BssSI CACGAG 1 cut(s) 434
BssT1I CCWWGG 1 cut(s) 36
Bst2BI CACGAG 1 cut(s) 434
Bst2UI CCWGG 2 cut(s) 196, 223
Bst4CI ACNGT 3 cut(s) 736, 926, 980
Bst6I CTCTTC 2 cut(s) 354, 601
BstACI GRCGYC 2 cut(s) 75, 602
BstBAI YACGTR 7 cut(s) 248, 271, 555, 564, 609, 646, 672
BstC8I GCNNGC 5 cut(s) 499, 522, 579, 1010, 1076
BstDEI CTNAG 2 cut(s) 21, 213
BstDSI CCRYGG 3 cut(s) 462, 525, 624
BstF5I GGATG 3 cut(s) 108, 301, 1089
BstFNI CGCG 5 cut(s) 153, 395, 477, 497, 626
BstHHI GCGC 3 cut(s) 155, 397, 497
BstKTI GATC 4 cut(s) 685, 718, 876, 1002
BstMAI GTCTC 5 cut(s) 66, 139, 244, 884, 915
BstMBI GATC 4 cut(s) 682, 715, 873, 999
BstMWI GCNNNNNNNGC 4 cut(s) 11, 439, 494, 1120
BstNI CCWGG 2 cut(s) 196, 223
BstNSI RCATGY 3 cut(s) 361, 886, 1078
BstSCI CCNGG 3 cut(s) 194, 221, 258
BstUI CGCG 5 cut(s) 153, 395, 477, 497, 626
BstV2I GAAGAC 1 cut(s) 287
BsuRI GGCC 5 cut(s) 263, 471, 524, 623, 709
BtgI CCRYGG 3 cut(s) 462, 525, 624
BtrI CACGTC 3 cut(s) 133, 808, 1095
BtsCI GGATG 3 cut(s) 108, 301, 1089
BtsIMutI CAGTG 2 cut(s) 624, 1145
BveI ACCTGC 1 cut(s) 1020
Cac8I GCNNGC 5 cut(s) 499, 522, 579, 1010, 1076
CfoI GCGC 3 cut(s) 155, 397, 497
Cfr13I GGNCC 3 cut(s) 469, 707, 1068
Cfr42I CCGCGG 1 cut(s) 627
CseI GACGC 3 cut(s) 401, 591, 710
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 5 cut(s) 535, 643, 673, 712, 1133
CviAII CATG 5 cut(s) 358, 574, 883, 1042, 1075
CviQI GTAC 5 cut(s) 535, 643, 673, 712, 1133
DdeI CTNAG 2 cut(s) 21, 213
DpnI GATC 4 cut(s) 684, 717, 875, 1001
DpnII GATC 4 cut(s) 682, 715, 873, 999
DrdI GACNNNNNNGTC 1 cut(s) 455
DriI GACNNNNNGTC 1 cut(s) 850
DseDI GACNNNNNNGTC 1 cut(s) 455
EaeI YGGCCR 2 cut(s) 522, 621
Eam1104I CTCTTC 2 cut(s) 354, 601
Eam1105I GACNNNNNGTC 1 cut(s) 850
EarI CTCTTC 2 cut(s) 354, 601
EciI GGCGGA 1 cut(s) 815
Eco130I CCWWGG 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 1068
Eco72I CACGTG 5 cut(s) 248, 271, 555, 564, 609
EcoO109I RGGNCCY 1 cut(s) 1068
EcoRII CCWGG 2 cut(s) 194, 221
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
Esp3I CGTCTC 3 cut(s) 66, 139, 244
FaeI CATG 5 cut(s) 361, 577, 886, 1045, 1078
FaqI GGGAC 2 cut(s) 572, 1081
FatI CATG 5 cut(s) 357, 573, 882, 1041, 1074
FauI CCCGC 4 cut(s) 525, 586, 775, 1079
FblI GTMKAC 2 cut(s) 164, 279
Fnu4HI GCNGC 3 cut(s) 42, 624, 798
FokI GGATG 3 cut(s) 95, 288, 1096
Fsp4HI GCNGC 3 cut(s) 42, 624, 798
FspBI CTAG 2 cut(s) 284, 404
GlaI GCGC 3 cut(s) 154, 396, 496
GluI GCNGC 3 cut(s) 42, 624, 798
HaeIII GGCC 5 cut(s) 263, 471, 524, 623, 709
HapII CCGG 5 cut(s) 260, 308, 686, 887, 1115
HgaI GACGC 3 cut(s) 401, 591, 710
HhaI GCGC 3 cut(s) 155, 397, 497
Hin1I GRCGYC 2 cut(s) 75, 602
Hin1II CATG 5 cut(s) 361, 577, 886, 1045, 1078
Hin6I GCGC 3 cut(s) 153, 395, 495
HinP1I GCGC 3 cut(s) 153, 395, 495
HincII GTYRAC 2 cut(s) 280, 763
HindII GTYRAC 2 cut(s) 280, 763
HpaI GTTAAC 1 cut(s) 763
HpaII CCGG 5 cut(s) 260, 308, 686, 887, 1115
HphI GGTGA 2 cut(s) 890, 1102
Hpy166II GTNNAC 7 cut(s) 165, 208, 280, 606, 763, 1035, 1060
Hpy188I TCNGA 5 cut(s) 216, 726, 732, 985, 1142
Hpy188III TCNNGA 1 cut(s) 686
Hpy8I GTNNAC 7 cut(s) 165, 208, 280, 606, 763, 1035, 1060
Hpy99I CGWCG 9 cut(s) 206, 239, 395, 488, 736, 848, 851, 937, 1067
HpyAV CCTTC 3 cut(s) 378, 805, 904
HpyCH4III ACNGT 3 cut(s) 736, 926, 980
HpyCH4V TGCA 3 cut(s) 1029, 1078, 1123
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 439, 494, 1120
HpyF3I CTNAG 2 cut(s) 21, 213
Hsp92I GRCGYC 2 cut(s) 75, 602
Hsp92II CATG 5 cut(s) 361, 577, 886, 1045, 1078
HspAI GCGC 3 cut(s) 153, 395, 495
KflI GGGWCCC 1 cut(s) 1068
Kpn2I TCCGGA 1 cut(s) 685
KspAI GTTAAC 1 cut(s) 763
KspI CCGCGG 1 cut(s) 627
Kzo9I GATC 4 cut(s) 682, 715, 873, 999
LmnI GCTCC 5 cut(s) 338, 402, 749, 794, 1142
LweI GCATC 1 cut(s) 1042
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 2 cut(s) 284, 404
MaeIII GTNAC 7 cut(s) 7, 128, 413, 646, 667, 878, 1090
MalI GATC 4 cut(s) 684, 717, 875, 1001
MbiI CCGCTC 4 cut(s) 64, 333, 433, 797
MboI GATC 4 cut(s) 682, 715, 873, 999
MboII GAAGA 5 cut(s) 62, 65, 287, 341, 588
MhlI GDGCHC 2 cut(s) 662, 1147
MluCI AATT 3 cut(s) 98, 374, 1152
MlyI GAGTC 8 cut(s) 92, 301, 340, 421, 578, 587, 698, 860
MmeI TCCRAC 4 cut(s) 755, 837, 1008, 1120
MroI TCCGGA 1 cut(s) 685
MseI TTAA 2 cut(s) 189, 762
MspA1I CMGCKG 2 cut(s) 520, 626
MspI CCGG 5 cut(s) 260, 308, 686, 887, 1115
MspR9I CCNGG 3 cut(s) 196, 223, 260
MvaI CCWGG 2 cut(s) 196, 223
MvnI CGCG 5 cut(s) 153, 395, 477, 497, 626
MwoI GCNNNNNNNGC 4 cut(s) 11, 439, 494, 1120
NciI CCSGG 1 cut(s) 260
NdeII GATC 4 cut(s) 682, 715, 873, 999
NlaIII CATG 5 cut(s) 361, 577, 886, 1045, 1078
NlaIV GGNNCC 5 cut(s) 149, 227, 982, 1069, 1070
NmeAIII GCCGAG 1 cut(s) 641
NmuCI GTSAC 6 cut(s) 128, 413, 646, 667, 878, 1090
NspI RCATGY 3 cut(s) 361, 886, 1078
PaeI GCATGC 1 cut(s) 1078
PaqCI CACCTGC 1 cut(s) 1020
PcsI WCGNNNNNNNCGW 4 cut(s) 276, 560, 605, 668
PfeI GAWTC 2 cut(s) 727, 756
Pfl23II CGTACG 1 cut(s) 711
PflMI CCANNNNNTGG 1 cut(s) 699
PkrI GCNGC 3 cut(s) 43, 625, 799
PleI GAGTC 8 cut(s) 91, 300, 340, 420, 577, 587, 697, 859
PmaCI CACGTG 5 cut(s) 248, 271, 555, 564, 609
PmlI CACGTG 5 cut(s) 248, 271, 555, 564, 609
PpsI GAGTC 8 cut(s) 91, 300, 340, 420, 577, 587, 697, 859
Ppu21I YACGTR 7 cut(s) 248, 271, 555, 564, 609, 646, 672
PpuMI RGGWCCY 1 cut(s) 1068
Psp5II RGGWCCY 1 cut(s) 1068
Psp6I CCWGG 2 cut(s) 194, 221
PspCI CACGTG 5 cut(s) 248, 271, 555, 564, 609
PspGI CCWGG 2 cut(s) 194, 221
PspLI CGTACG 1 cut(s) 711
PspN4I GGNNCC 5 cut(s) 149, 227, 982, 1069, 1070
PspPI GGNCC 3 cut(s) 469, 707, 1068
PspPPI RGGWCCY 1 cut(s) 1068
RsaI GTAC 5 cut(s) 536, 644, 674, 713, 1134
RsaNI GTAC 5 cut(s) 535, 643, 673, 712, 1133
SacII CCGCGG 1 cut(s) 627
SalI GTCGAC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 189, 762
SatI GCNGC 3 cut(s) 42, 624, 798
Sau3AI GATC 4 cut(s) 682, 715, 873, 999
Sau96I GGNCC 3 cut(s) 469, 707, 1068
SchI GAGTC 8 cut(s) 92, 301, 340, 421, 578, 587, 698, 860
ScrFI CCNGG 3 cut(s) 196, 223, 260
SduI GDGCHC 2 cut(s) 662, 1147
SexAI ACCWGGT 1 cut(s) 221
SfaNI GCATC 1 cut(s) 1042
Sfr303I CCGCGG 1 cut(s) 627
SgrBI CCGCGG 1 cut(s) 627
SinI GGWCC 1 cut(s) 1068
SphI GCATGC 1 cut(s) 1078
Sse9I AATT 3 cut(s) 98, 374, 1152
SspMI CTAG 2 cut(s) 284, 404
StyD4I CCNGG 3 cut(s) 194, 221, 258
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 3 cut(s) 736, 926, 980
TaqI TCGA 4 cut(s) 279, 344, 390, 941
TasI AATT 3 cut(s) 98, 374, 1152
TauI GCSGC 3 cut(s) 44, 626, 800
TfiI GAWTC 2 cut(s) 727, 756
Tru1I TTAA 2 cut(s) 189, 762
Tru9I TTAA 2 cut(s) 189, 762
TscAI CASTG 2 cut(s) 624, 1152
TseFI GTSAC 6 cut(s) 128, 413, 646, 667, 878, 1090
Tsp45I GTSAC 6 cut(s) 128, 413, 646, 667, 878, 1090
TspDTI ATGAA 1 cut(s) 287
TspGWI ACGGA 6 cut(s) 76, 84, 161, 304, 665, 736
TspRI CASTG 2 cut(s) 624, 1152
Van91I CCANNNNNTGG 1 cut(s) 699
VpaK11BI GGWCC 1 cut(s) 1068
XapI RAATTY 2 cut(s) 374, 1152
XceI RCATGY 3 cut(s) 361, 886, 1078
XmiI GTMKAC 2 cut(s) 164, 279
XspI CTAG 2 cut(s) 284, 404
ZraI GACGTC 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.