Rroxscaffold_5G00382880

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010437
Physical Location & Seq
Forward (+)
62639021 .. 62640064
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00382880.1

Sequence Viewer

Length: 1044 bp
ATGGCAGTAACGGCGAACCATAAGCAGAACCAGACCAAGGCGGCGGTTGAAGAAGAAGCACCGCTCTACGGAGACGTCTTGGAGTCCGTGCTATCCAAATTACACCTCATCCATCTCCTATCGGCGTGTCACGTCTCAAAGTCTTGGAACCGCTCCGTTTCGTCTACCCTCCGTTTCTCTAACCGAGTTAAACCCTGGCTCGTCGTCCACACTCAGACCACCAGGTTCCCCTACCTCGTCTCCGCACGTGCTTTCGACCCGGCCTCGCACGTGTGGGTCGACGTCGAACGGAGTCTTCATCATCCTCCGGTCAAGTCCATCTCCACCTTACGCTCCGCTCATTCGACTCTTCTCTACATGCTTTCCCCTTCCAAATTCGCTTTCTCCCTCGACGCGCTCCACCTAGAGTGGAGTCACGCAAACACGCCTCCGCTCGTGTGGCGAACTGATCCGATTGTCGCCGTGGTGGGCCACCGCGTAGTAGTCGCAGGCGGCGCGTGCGACTACGAGGACGACCCGCTGGCCGTGGAAATGTACGATGTCAAGGTTGGCACGTGGGACACGTGCGAGTCCATGCCCGCCATTTTCAAAGACTCTTCGGCGTCCACGTGGCTGTCTGTAGCCGCGGACGAGAGGAGAATGTACGTGATGGAGAAGTGCTCGGCTGTCACGTACTCGTTTGATCCGGAGTCCAAGAAGTGGTTTGGGCCGTACGATCTGCTTCCGAATCCGACGGTGTTTGGCTCCGTGATTGGATTCGTTAACGGGCGTTTGGTTTTGGCGGGAGTTGTGGGAGAGGCGGAGGACGTGAAGGGAGTGACGTTGTGGGAAGTGGAAGTCAGCGACGAGGAGTCGTTGGAGTTGAAAGAGATGATCGGTGACATGCCGGAGACAATGGTGGAGAAGTTGAAGGGCGAGAGCGACTGTGAGCCGTCGTTTTCGATGAGCTATATGGGAGGTCTGGTGTGTTTTCACAATGGTTCCGACCCGAGCGAGTTGATCCTCTGCGAGCTTGAGGATGGGGGTGCAGGTGGACCAGCATAA

Protein Analysis

347

Amino Acids

38.21

Weight (kDa)

5.04

Isoelectric Point (pI)

44.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 122 - 234 3.9e-07 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1019
AatII GACGTC 2 cut(s) 78, 285
Acc36I ACCTGC 1 cut(s) 1019
AccB7I CCANNNNNTGG 1 cut(s) 699
AccBSI CCGCTC 4 cut(s) 64, 153, 338, 433
AccI GTMKAC 2 cut(s) 164, 279
AccII CGCG 4 cut(s) 395, 477, 497, 626
AccIII TCCGGA 1 cut(s) 685
AclWI GGATC 3 cut(s) 443, 677, 994
AcoI YGGCCR 1 cut(s) 522
AcsI RAATTY 1 cut(s) 374
AcvI CACGTG 5 cut(s) 248, 271, 555, 564, 609
AcyI GRCGYC 3 cut(s) 75, 282, 602
AfaI GTAC 4 cut(s) 536, 644, 674, 713
AfiI CCNNNNNNNGG 3 cut(s) 37, 68, 699
AflIII ACRYGT 2 cut(s) 270, 561
AgsI TTSAA 4 cut(s) 50, 589, 865, 910
AhdI GACNNNNNGTC 1 cut(s) 850
AjiI CACGTC 2 cut(s) 133, 808
AjnI CCWGG 2 cut(s) 194, 221
AjuI GAANNNNNNNTTGG 2 cut(s) 365, 397
AluBI AGCT 2 cut(s) 948, 1012
AluI AGCT 2 cut(s) 948, 1012
Alw21I GWGCWC 1 cut(s) 662
Alw26I GTCTC 4 cut(s) 66, 139, 244, 884
AlwI GGATC 3 cut(s) 443, 677, 994
Ama87I CYCGRG 1 cut(s) 988
Aor13HI TCCGGA 1 cut(s) 685
AoxI GGCC 4 cut(s) 261, 469, 522, 707
ApoI RAATTY 1 cut(s) 374
AspLEI GCGC 2 cut(s) 397, 497
AspS9I GGNCC 3 cut(s) 469, 707, 1034
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 1 cut(s) 890
AvaI CYCGRG 1 cut(s) 988
AvaII GGWCC 1 cut(s) 1034
BauI CACGAG 1 cut(s) 434
BbrPI CACGTG 5 cut(s) 248, 271, 555, 564, 609
BbsI GAAGAC 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 662
BccI CCATC 4 cut(s) 120, 326, 643, 1013
BceAI ACGGC 5 cut(s) 27, 446, 509, 694, 916
BciT130I CCWGG 2 cut(s) 196, 223
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 4 cut(s) 66, 139, 244, 884
BfaI CTAG 1 cut(s) 404
BfmI CTRYAG 1 cut(s) 618
BfuAI ACCTGC 1 cut(s) 1019
BisI GCNGC 3 cut(s) 42, 493, 624
BlsI GCNGC 3 cut(s) 43, 494, 625
Bme1390I CCNGG 3 cut(s) 196, 223, 260
Bme18I GGWCC 1 cut(s) 1034
BmeRI GACNNNNNGTC 1 cut(s) 850
BmeT110I CYCGRG 1 cut(s) 988
BmgBI CACGTC 2 cut(s) 133, 808
BmgT120I GGNCC 3 cut(s) 469, 707, 1034
BmiI GGNNCC 4 cut(s) 149, 227, 745, 982
BmrFI CCNGG 3 cut(s) 196, 223, 260
BpiI GAAGAC 1 cut(s) 287
BplI GAGNNNNNCTC 2 cut(s) 644, 676
BpuEI CTTGAG 1 cut(s) 1034
BpuMI CCSGG 1 cut(s) 260
BsaAI YACGTR 7 cut(s) 248, 271, 555, 564, 609, 646, 672
BsaHI GRCGYC 3 cut(s) 75, 282, 602
BsaJI CCNNGG 5 cut(s) 36, 194, 462, 525, 624
BsaWI WCCGGW 2 cut(s) 307, 685
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 948, 978
Bsc4I CCNNNNNNNGG 3 cut(s) 37, 68, 699
BseAI TCCGGA 1 cut(s) 685
BseBI CCWGG 2 cut(s) 196, 223
BseDI CCNNGG 5 cut(s) 36, 194, 462, 525, 624
BseGI GGATG 3 cut(s) 108, 301, 1024
BseLI CCNNNNNNNGG 3 cut(s) 37, 68, 699
BseMII CTCAG 1 cut(s) 227
BseRI GAGGAG 2 cut(s) 649, 863
Bsh1236I CGCG 4 cut(s) 395, 477, 497, 626
BshFI GGCC 4 cut(s) 263, 471, 524, 709
BsiHKAI GWGCWC 1 cut(s) 662
BsiHKCI CYCGRG 1 cut(s) 988
BsiSI CCGG 4 cut(s) 260, 308, 686, 887
BsiWI CGTACG 1 cut(s) 711
BslFI GGGAC 1 cut(s) 572
BslI CCNNNNNNNGG 3 cut(s) 37, 68, 699
BsmAI GTCTC 4 cut(s) 66, 139, 244, 884
BsmBI CGTCTC 3 cut(s) 66, 139, 244
BsmFI GGGAC 1 cut(s) 572
BsnI GGCC 4 cut(s) 263, 471, 524, 709
BsoBI CYCGRG 1 cut(s) 988
Bsp1286I GDGCHC 1 cut(s) 662
Bsp13I TCCGGA 1 cut(s) 685
Bsp143I GATC 5 cut(s) 448, 682, 715, 873, 999
BspANI GGCC 4 cut(s) 263, 471, 524, 709
BspCNI CTCAG 1 cut(s) 226
BspEI TCCGGA 1 cut(s) 685
BspFNI CGCG 4 cut(s) 395, 477, 497, 626
BspLI GGNNCC 4 cut(s) 149, 227, 745, 982
BspMI ACCTGC 1 cut(s) 1019
BspPI GGATC 3 cut(s) 443, 677, 994
BsrBI CCGCTC 4 cut(s) 64, 153, 338, 433
BssECI CCNNGG 5 cut(s) 36, 194, 462, 525, 624
BssMI GATC 5 cut(s) 448, 682, 715, 873, 999
BssNI GRCGYC 3 cut(s) 75, 282, 602
BssSI CACGAG 1 cut(s) 434
BssT1I CCWWGG 1 cut(s) 36
Bst2BI CACGAG 1 cut(s) 434
Bst2UI CCWGG 2 cut(s) 196, 223
Bst4CI ACNGT 2 cut(s) 736, 926
Bst6I CTCTTC 2 cut(s) 354, 601
BstACI GRCGYC 3 cut(s) 75, 282, 602
BstBAI YACGTR 7 cut(s) 248, 271, 555, 564, 609, 646, 672
BstC8I GCNNGC 5 cut(s) 490, 499, 522, 579, 1010
BstDEI CTNAG 1 cut(s) 213
BstDSI CCRYGG 3 cut(s) 462, 525, 624
BstF5I GGATG 3 cut(s) 108, 301, 1024
BstFNI CGCG 4 cut(s) 395, 477, 497, 626
BstHHI GCGC 2 cut(s) 397, 497
BstKTI GATC 5 cut(s) 451, 685, 718, 876, 1002
BstMAI GTCTC 4 cut(s) 66, 139, 244, 884
BstMBI GATC 5 cut(s) 448, 682, 715, 873, 999
BstMWI GCNNNNNNNGC 4 cut(s) 11, 439, 494, 498
BstNI CCWGG 2 cut(s) 196, 223
BstNSI RCATGY 2 cut(s) 361, 886
BstSCI CCNGG 3 cut(s) 194, 221, 258
BstSFI CTRYAG 1 cut(s) 618
BstUI CGCG 4 cut(s) 395, 477, 497, 626
BstV2I GAAGAC 1 cut(s) 287
BsuRI GGCC 4 cut(s) 263, 471, 524, 709
BtgI CCRYGG 3 cut(s) 462, 525, 624
BtrI CACGTC 2 cut(s) 133, 808
BtsCI GGATG 3 cut(s) 108, 301, 1024
BveI ACCTGC 1 cut(s) 1019
Cac8I GCNNGC 5 cut(s) 490, 499, 522, 579, 1010
CfoI GCGC 2 cut(s) 397, 497
Cfr13I GGNCC 3 cut(s) 469, 707, 1034
Cfr42I CCGCGG 1 cut(s) 627
CseI GACGC 2 cut(s) 401, 591
CsiI ACCWGGT 1 cut(s) 221
Csp6I GTAC 4 cut(s) 535, 643, 673, 712
CviAII CATG 3 cut(s) 358, 574, 883
CviQI GTAC 4 cut(s) 535, 643, 673, 712
DdeI CTNAG 1 cut(s) 213
DpnI GATC 5 cut(s) 450, 684, 717, 875, 1001
DpnII GATC 5 cut(s) 448, 682, 715, 873, 999
DriI GACNNNNNGTC 1 cut(s) 850
EaeI YGGCCR 1 cut(s) 522
Eam1104I CTCTTC 2 cut(s) 354, 601
Eam1105I GACNNNNNGTC 1 cut(s) 850
EarI CTCTTC 2 cut(s) 354, 601
EciI GGCGGA 1 cut(s) 815
Eco130I CCWWGG 1 cut(s) 36
Eco47I GGWCC 1 cut(s) 1034
Eco72I CACGTG 5 cut(s) 248, 271, 555, 564, 609
Eco88I CYCGRG 1 cut(s) 988
EcoRII CCWGG 2 cut(s) 194, 221
EcoT14I CCWWGG 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 36
Esp3I CGTCTC 3 cut(s) 66, 139, 244
FaeI CATG 3 cut(s) 361, 577, 886
FaiI YATR 7 cut(s) 21, 359, 575, 884, 951, 953, 1042
FaqI GGGAC 1 cut(s) 572
FatI CATG 3 cut(s) 357, 573, 882
FauI CCCGC 3 cut(s) 525, 586, 775
FblI GTMKAC 2 cut(s) 164, 279
Fnu4HI GCNGC 3 cut(s) 42, 493, 624
FokI GGATG 3 cut(s) 95, 288, 1031
Fsp4HI GCNGC 3 cut(s) 42, 493, 624
FspBI CTAG 1 cut(s) 404
GlaI GCGC 2 cut(s) 396, 496
GluI GCNGC 3 cut(s) 42, 493, 624
HaeIII GGCC 4 cut(s) 263, 471, 524, 709
HapII CCGG 4 cut(s) 260, 308, 686, 887
HgaI GACGC 2 cut(s) 401, 591
HhaI GCGC 2 cut(s) 397, 497
Hin1I GRCGYC 3 cut(s) 75, 282, 602
Hin1II CATG 3 cut(s) 361, 577, 886
Hin6I GCGC 2 cut(s) 395, 495
HinP1I GCGC 2 cut(s) 395, 495
HincII GTYRAC 2 cut(s) 280, 763
HindII GTYRAC 2 cut(s) 280, 763
HpaI GTTAAC 1 cut(s) 763
HpaII CCGG 4 cut(s) 260, 308, 686, 887
HphI GGTGA 1 cut(s) 890
Hpy166II GTNNAC 6 cut(s) 165, 208, 280, 606, 763, 1034
Hpy188I TCNGA 5 cut(s) 216, 453, 726, 732, 985
Hpy188III TCNNGA 1 cut(s) 686
Hpy8I GTNNAC 6 cut(s) 165, 208, 280, 606, 763, 1034
Hpy99I CGWCG 7 cut(s) 206, 284, 287, 395, 736, 848, 937
HpyAV CCTTC 3 cut(s) 378, 805, 904
HpyCH4III ACNGT 2 cut(s) 736, 926
HpyCH4V TGCA 1 cut(s) 1028
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 439, 494, 498
HpyF3I CTNAG 1 cut(s) 213
Hsp92I GRCGYC 3 cut(s) 75, 282, 602
Hsp92II CATG 3 cut(s) 361, 577, 886
HspAI GCGC 2 cut(s) 395, 495
Kpn2I TCCGGA 1 cut(s) 685
KspAI GTTAAC 1 cut(s) 763
KspI CCGCGG 1 cut(s) 627
Kzo9I GATC 5 cut(s) 448, 682, 715, 873, 999
LmnI GCTCC 4 cut(s) 158, 338, 402, 749
MabI ACCWGGT 1 cut(s) 221
MaeI CTAG 1 cut(s) 404
MaeIII GTNAC 6 cut(s) 7, 128, 413, 667, 817, 878
MalI GATC 5 cut(s) 450, 684, 717, 875, 1001
MbiI CCGCTC 4 cut(s) 64, 153, 338, 433
MboI GATC 5 cut(s) 448, 682, 715, 873, 999
MboII GAAGA 5 cut(s) 62, 65, 287, 341, 588
MhlI GDGCHC 1 cut(s) 662
MluCI AATT 2 cut(s) 98, 374
MlyI GAGTC 8 cut(s) 92, 301, 340, 421, 578, 587, 698, 860
MmeI TCCRAC 3 cut(s) 755, 837, 1008
MroI TCCGGA 1 cut(s) 685
MseI TTAA 2 cut(s) 189, 762
MspA1I CMGCKG 2 cut(s) 520, 626
MspI CCGG 4 cut(s) 260, 308, 686, 887
MspR9I CCNGG 3 cut(s) 196, 223, 260
MvaI CCWGG 2 cut(s) 196, 223
MvnI CGCG 4 cut(s) 395, 477, 497, 626
MwoI GCNNNNNNNGC 4 cut(s) 11, 439, 494, 498
NciI CCSGG 1 cut(s) 260
NdeII GATC 5 cut(s) 448, 682, 715, 873, 999
NlaIII CATG 3 cut(s) 361, 577, 886
NlaIV GGNNCC 4 cut(s) 149, 227, 745, 982
NmeAIII GCCGAG 1 cut(s) 641
NmuCI GTSAC 5 cut(s) 128, 413, 667, 817, 878
NspI RCATGY 2 cut(s) 361, 886
PaqCI CACCTGC 1 cut(s) 1019
PcsI WCGNNNNNNNCGW 4 cut(s) 276, 560, 605, 668
PfeI GAWTC 2 cut(s) 727, 756
Pfl23II CGTACG 1 cut(s) 711
PflMI CCANNNNNTGG 1 cut(s) 699
PkrI GCNGC 3 cut(s) 43, 494, 625
PleI GAGTC 8 cut(s) 91, 300, 340, 420, 577, 587, 697, 859
PmaCI CACGTG 5 cut(s) 248, 271, 555, 564, 609
PmlI CACGTG 5 cut(s) 248, 271, 555, 564, 609
PpsI GAGTC 8 cut(s) 91, 300, 340, 420, 577, 587, 697, 859
Ppu21I YACGTR 7 cut(s) 248, 271, 555, 564, 609, 646, 672
Psp6I CCWGG 2 cut(s) 194, 221
PspCI CACGTG 5 cut(s) 248, 271, 555, 564, 609
PspGI CCWGG 2 cut(s) 194, 221
PspLI CGTACG 1 cut(s) 711
PspN4I GGNNCC 4 cut(s) 149, 227, 745, 982
PspPI GGNCC 3 cut(s) 469, 707, 1034
RsaI GTAC 4 cut(s) 536, 644, 674, 713
RsaNI GTAC 4 cut(s) 535, 643, 673, 712
SacII CCGCGG 1 cut(s) 627
SalI GTCGAC 1 cut(s) 278
SaqAI TTAA 2 cut(s) 189, 762
SatI GCNGC 3 cut(s) 42, 493, 624
Sau3AI GATC 5 cut(s) 448, 682, 715, 873, 999
Sau96I GGNCC 3 cut(s) 469, 707, 1034
SchI GAGTC 8 cut(s) 92, 301, 340, 421, 578, 587, 698, 860
ScrFI CCNGG 3 cut(s) 196, 223, 260
SduI GDGCHC 1 cut(s) 662
SexAI ACCWGGT 1 cut(s) 221
SfcI CTRYAG 1 cut(s) 618
Sfr303I CCGCGG 1 cut(s) 627
SgrBI CCGCGG 1 cut(s) 627
SinI GGWCC 1 cut(s) 1034
SmlI CTYRAG 1 cut(s) 1013
SmoI CTYRAG 1 cut(s) 1013
Sse9I AATT 2 cut(s) 98, 374
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 3 cut(s) 194, 221, 258
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 2 cut(s) 736, 926
TaqI TCGA 6 cut(s) 255, 279, 285, 344, 390, 941
TasI AATT 2 cut(s) 98, 374
TauI GCSGC 3 cut(s) 44, 495, 626
TfiI GAWTC 2 cut(s) 727, 756
Tru1I TTAA 2 cut(s) 189, 762
Tru9I TTAA 2 cut(s) 189, 762
TseFI GTSAC 5 cut(s) 128, 413, 667, 817, 878
Tsp45I GTSAC 5 cut(s) 128, 413, 667, 817, 878
TspDTI ATGAA 1 cut(s) 287
TspGWI ACGGA 6 cut(s) 76, 84, 145, 161, 304, 736
Van91I CCANNNNNTGG 1 cut(s) 699
VpaK11BI GGWCC 1 cut(s) 1034
XapI RAATTY 1 cut(s) 374
XceI RCATGY 2 cut(s) 361, 886
XmiI GTMKAC 2 cut(s) 164, 279
XspI CTAG 1 cut(s) 404
ZraI GACGTC 2 cut(s) 76, 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.