FvH4_4g32970

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010437
Physical Location & Seq
Forward (+)
31375578 .. 31377243
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g32970.t1

Sequence Viewer

Length: 1176 bp
ATGGCAGTAGTGGCGAAACCTAATCAGAACCAGACCAAAACGGCGGTTGTTGAAGAAGAAGCTCCGCTCTACGGAGACGTCTTGGAGTCCGTCCTCTCCAAACTACACCTCATCCATCTCCTACCGGCCTATCACGTCTCCAAGTCGTGGCACCGCGCCGTTTCCTCCACCCTCCGTTTCGCTAACCGAGTTAAACCCTGGCTCGTCGTCCACACTCAGACCACCAGATTCCCTTACGTCGTCTCCTCACGTGCTTACGACCCTGCCTCGCACGTGTGGCTCGACGTCGACCACCGGAGTCTTCATCATTCTCCGGTCAAGTCCATCTCCACCCTCCGCTCCTCTCACTCGACTCTTCTCTACATGCTTTCCCCTTCCAAATTCGCATTCTCCCTCGACGCCCTCCACTTCGAGTGGAGCCACGCAAACACGCCTCCGCTAGTATGGCGAACTGACCCTATTGTCGCGGTGGTGGGCCACCGCGTCGTCGTTGCAGGCGGCGCGTGCGATTACGAGGACGACCCGCTCGCTGTAGAAATGTACGATGTCAAGGCTGGCACGTGGGATACGTGCGACTCTATGCCCGCCATTTTCAAAGACTCCGCGGCCTCCACTTGGCTATCGGTGGCCGCGGAGGGTAGTAGAATGTACGTGACGGAGAAGTGCTCGGCCGTCACGTACTCATTCGATCCGGAGTCCAAGACGTGGTTTGGACCGTACGTTCTCCGTCCGACTCCGACGGTGTTTAGCTCCTTGATTGGATTCGTTAACGGGCGTTTGGTTTTGGCGGGAGTTGTGGGAGAGGCGGAAGACGTGAAGGGGGTGAAGCTGTGGGAGGTGAAAATGAGCGACGTCGACGAGTCGTTGGAGTTGGGAGAGATGATATTGATCGGTGACATGCCGGAGATGATGGTGGAGAAGTTGAAGGGCGAGAGCTATTATGAGCCGTCGATTTCAATGAGCTGCATGGGAGGTTTGGTGTGTTTTCACAACGCTTCCGACCCGAGCGAGTTGACCCTCTGCGAGGTTGAGAAGGGAGGGTGCAGGTGGAGTAGCGTAAGGAACGACGTCGTGAACGATGGGACACGGTTGCAGAGGATGGTGGTGACGTGGGCCGATGTTGGTTTGCCGGAGTTGCAGAAAGCTGTACAAGTCGGAGCACTGAAAATTGTATGA

Protein Analysis

392

Amino Acids

43.25

Weight (kDa)

5.69

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_FKB95 PF25210 125 - 236 6.7e-06 FKB95, Kelch-repeats domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1035
AasI GACNNNNNNGTC 1 cut(s) 461
AatII GACGTC 4 cut(s) 81, 288, 855, 1071
Acc36I ACCTGC 1 cut(s) 1035
AccB1I GGYRCC 1 cut(s) 150
AccB7I CCANNNNNTGG 2 cut(s) 147, 705
AccBSI CCGCTC 3 cut(s) 67, 339, 526
AccI GTMKAC 2 cut(s) 288, 855
AccII CGCG 6 cut(s) 156, 467, 483, 503, 605, 632
AccIII TCCGGA 1 cut(s) 691
AclWI GGATC 1 cut(s) 683
AcoI YGGCCR 2 cut(s) 627, 669
AcsI RAATTY 1 cut(s) 380
AcvI CACGTG 3 cut(s) 251, 274, 561
AcyI GRCGYC 5 cut(s) 78, 285, 399, 852, 1068
AfaI GTAC 5 cut(s) 542, 650, 680, 719, 1149
AfiI CCNNNNNNNGG 5 cut(s) 71, 147, 615, 705, 1024
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 4 cut(s) 53, 595, 925, 957
AjiI CACGTC 4 cut(s) 136, 705, 814, 1110
AjnI CCWGG 1 cut(s) 197
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AloI GAACNNNNNNTCC 2 cut(s) 705, 737
AluBI AGCT 6 cut(s) 62, 750, 829, 936, 963, 1145
AluI AGCT 6 cut(s) 62, 750, 829, 936, 963, 1145
Alw21I GWGCWC 2 cut(s) 668, 1162
Alw26I GTCTC 3 cut(s) 69, 142, 247
AlwI GGATC 1 cut(s) 683
Ama87I CYCGRG 1 cut(s) 1003
Aor13HI TCCGGA 1 cut(s) 691
AoxI GGCC 6 cut(s) 126, 475, 606, 627, 669, 1113
ApeKI GCWGC 1 cut(s) 963
ApoI RAATTY 1 cut(s) 380
AspLEI GCGC 2 cut(s) 158, 503
AspS9I GGNCC 3 cut(s) 475, 713, 1113
AsuHPI GGTGA 4 cut(s) 835, 850, 905, 1117
AvaI CYCGRG 1 cut(s) 1003
AvaII GGWCC 1 cut(s) 713
BanI GGYRCC 1 cut(s) 150
BbrPI CACGTG 3 cut(s) 251, 274, 561
BbsI GAAGAC 2 cut(s) 293, 816
Bbv12I GWGCWC 2 cut(s) 668, 1162
BbvI GCAGC 1 cut(s) 950
BccI CCATC 5 cut(s) 123, 332, 904, 1073, 1093
BceAI ACGGC 4 cut(s) 57, 143, 656, 931
BciT130I CCWGG 1 cut(s) 199
BciVI GTATCC 1 cut(s) 559
BcoDI GTCTC 3 cut(s) 69, 142, 247
BfaI CTAG 1 cut(s) 440
BfmI CTRYAG 1 cut(s) 531
BfuAI ACCTGC 1 cut(s) 1035
BfuI GTATCC 1 cut(s) 559
BisI GCNGC 4 cut(s) 499, 606, 630, 964
BlsI GCNGC 4 cut(s) 500, 607, 631, 965
Bme1390I CCNGG 1 cut(s) 199
Bme18I GGWCC 1 cut(s) 713
BmeT110I CYCGRG 1 cut(s) 1003
BmgBI CACGTC 4 cut(s) 136, 705, 814, 1110
BmgT120I GGNCC 3 cut(s) 475, 713, 1113
BmiI GGNNCC 2 cut(s) 152, 419
BmrFI CCNGG 1 cut(s) 199
BpiI GAAGAC 2 cut(s) 293, 816
BplI GAGNNNNNCTC 2 cut(s) 650, 682
BsaAI YACGTR 6 cut(s) 251, 274, 561, 570, 652, 678
BsaBI GATNNNNATC 1 cut(s) 887
BsaHI GRCGYC 5 cut(s) 78, 285, 399, 852, 1068
BsaJI CCNNGG 3 cut(s) 197, 603, 630
BsaWI WCCGGW 3 cut(s) 294, 313, 691
BsaXI ACNNNNNCTCC 4 cut(s) 227, 257, 963, 993
Bsc4I CCNNNNNNNGG 5 cut(s) 71, 147, 615, 705, 1024
Bse118I RCCGGY 1 cut(s) 124
Bse8I GATNNNNATC 1 cut(s) 887
BseAI TCCGGA 1 cut(s) 691
BseBI CCWGG 1 cut(s) 199
BseDI CCNNGG 3 cut(s) 197, 603, 630
BseGI GGATG 2 cut(s) 111, 1104
BseJI GATNNNNATC 1 cut(s) 887
BseLI CCNNNNNNNGG 5 cut(s) 71, 147, 615, 705, 1024
BseMII CTCAG 1 cut(s) 230
BseRI GAGGAG 2 cut(s) 235, 331
BseX3I CGGCCG 1 cut(s) 669
BseXI GCAGC 1 cut(s) 950
BsgI GTGCAG 1 cut(s) 1063
Bsh1236I CGCG 6 cut(s) 156, 467, 483, 503, 605, 632
Bsh1285I CGRYCG 1 cut(s) 672
BshFI GGCC 6 cut(s) 128, 477, 608, 629, 671, 1115
BshNI GGYRCC 1 cut(s) 150
BsiEI CGRYCG 1 cut(s) 672
BsiHKAI GWGCWC 2 cut(s) 668, 1162
BsiHKCI CYCGRG 1 cut(s) 1003
BsiSI CCGG 6 cut(s) 125, 295, 314, 692, 902, 1130
BsiWI CGTACG 1 cut(s) 717
BslFI GGGAC 1 cut(s) 1096
BslI CCNNNNNNNGG 5 cut(s) 71, 147, 615, 705, 1024
BsmAI GTCTC 3 cut(s) 69, 142, 247
BsmBI CGTCTC 3 cut(s) 69, 142, 247
BsmFI GGGAC 1 cut(s) 1096
BsmI GAATGC 1 cut(s) 386
BsnI GGCC 6 cut(s) 128, 477, 608, 629, 671, 1115
BsoBI CYCGRG 1 cut(s) 1003
Bsp1286I GDGCHC 2 cut(s) 668, 1162
Bsp13I TCCGGA 1 cut(s) 691
Bsp1407I TGTACA 1 cut(s) 1147
Bsp143I GATC 2 cut(s) 688, 888
BspANI GGCC 6 cut(s) 128, 477, 608, 629, 671, 1115
BspCNI CTCAG 1 cut(s) 229
BspEI TCCGGA 1 cut(s) 691
BspFNI CGCG 6 cut(s) 156, 467, 483, 503, 605, 632
BspLI GGNNCC 2 cut(s) 152, 419
BspMI ACCTGC 1 cut(s) 1035
BspPI GGATC 1 cut(s) 683
BspT107I GGYRCC 1 cut(s) 150
BsrBI CCGCTC 3 cut(s) 67, 339, 526
BsrFI RCCGGY 1 cut(s) 124
BsrGI TGTACA 1 cut(s) 1147
BssAI RCCGGY 1 cut(s) 124
BssECI CCNNGG 3 cut(s) 197, 603, 630
BssMI GATC 2 cut(s) 688, 888
BssNI GRCGYC 5 cut(s) 78, 285, 399, 852, 1068
Bst2UI CCWGG 1 cut(s) 199
Bst4CI ACNGT 3 cut(s) 717, 742, 1089
Bst6I CTCTTC 1 cut(s) 360
BstACI GRCGYC 5 cut(s) 78, 285, 399, 852, 1068
BstAUI TGTACA 1 cut(s) 1147
BstBAI YACGTR 6 cut(s) 251, 274, 561, 570, 652, 678
BstC8I GCNNGC 5 cut(s) 496, 505, 528, 556, 585
BstDEI CTNAG 1 cut(s) 216
BstDSI CCRYGG 2 cut(s) 603, 630
BstENI CCTNNNNNAGG 1 cut(s) 1022
BstF5I GGATG 2 cut(s) 111, 1104
BstFNI CGCG 6 cut(s) 156, 467, 483, 503, 605, 632
BstHHI GCGC 2 cut(s) 158, 503
BstKTI GATC 2 cut(s) 691, 891
BstMAI GTCTC 3 cut(s) 69, 142, 247
BstMBI GATC 2 cut(s) 688, 888
BstMCI CGRYCG 1 cut(s) 672
BstMWI GCNNNNNNNGC 6 cut(s) 11, 277, 445, 500, 504, 1135
BstNI CCWGG 1 cut(s) 199
BstNSI RCATGY 2 cut(s) 367, 901
BstSCI CCNGG 1 cut(s) 197
BstSFI CTRYAG 1 cut(s) 531
BstUI CGCG 6 cut(s) 156, 467, 483, 503, 605, 632
BstV1I GCAGC 1 cut(s) 950
BstV2I GAAGAC 2 cut(s) 293, 816
BstZI CGGCCG 1 cut(s) 669
BsuI GTATCC 1 cut(s) 559
BsuRI GGCC 6 cut(s) 128, 477, 608, 629, 671, 1115
BtgI CCRYGG 2 cut(s) 603, 630
BtrI CACGTC 4 cut(s) 136, 705, 814, 1110
BtsCI GGATG 2 cut(s) 111, 1104
BtsIMutI CAGTG 1 cut(s) 1160
BveI ACCTGC 1 cut(s) 1035
Cac8I GCNNGC 5 cut(s) 496, 505, 528, 556, 585
CfoI GCGC 2 cut(s) 158, 503
Cfr10I RCCGGY 1 cut(s) 124
Cfr13I GGNCC 3 cut(s) 475, 713, 1113
Cfr42I CCGCGG 2 cut(s) 606, 633
CseI GACGC 2 cut(s) 407, 472
Csp6I GTAC 5 cut(s) 541, 649, 679, 718, 1148
CviAII CATG 3 cut(s) 364, 898, 967
CviQI GTAC 5 cut(s) 541, 649, 679, 718, 1148
DdeI CTNAG 1 cut(s) 216
DpnI GATC 2 cut(s) 690, 890
DpnII GATC 2 cut(s) 688, 888
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 2 cut(s) 627, 669
EagI CGGCCG 1 cut(s) 669
Eam1104I CTCTTC 1 cut(s) 360
EarI CTCTTC 1 cut(s) 360
EciI GGCGGA 1 cut(s) 821
EclXI CGGCCG 1 cut(s) 669
Eco47I GGWCC 1 cut(s) 713
Eco52I CGGCCG 1 cut(s) 669
Eco72I CACGTG 3 cut(s) 251, 274, 561
Eco88I CYCGRG 1 cut(s) 1003
EcoNI CCTNNNNNAGG 1 cut(s) 1022
EcoRII CCWGG 1 cut(s) 197
Esp3I CGTCTC 3 cut(s) 69, 142, 247
FaeI CATG 3 cut(s) 367, 901, 970
FaiI YATR 7 cut(s) 365, 445, 581, 899, 942, 968, 1174
FaqI GGGAC 1 cut(s) 1096
FatI CATG 3 cut(s) 363, 897, 966
FauI CCCGC 3 cut(s) 531, 592, 781
FblI GTMKAC 2 cut(s) 288, 855
Fnu4HI GCNGC 4 cut(s) 499, 606, 630, 964
FokI GGATG 2 cut(s) 98, 1111
Fsp4HI GCNGC 4 cut(s) 499, 606, 630, 964
FspBI CTAG 1 cut(s) 440
GlaI GCGC 2 cut(s) 157, 502
GluI GCNGC 4 cut(s) 499, 606, 630, 964
HaeIII GGCC 6 cut(s) 128, 477, 608, 629, 671, 1115
HapII CCGG 6 cut(s) 125, 295, 314, 692, 902, 1130
HgaI GACGC 2 cut(s) 407, 472
HhaI GCGC 2 cut(s) 158, 503
Hin1I GRCGYC 5 cut(s) 78, 285, 399, 852, 1068
Hin1II CATG 3 cut(s) 367, 901, 970
Hin6I GCGC 2 cut(s) 156, 501
HinP1I GCGC 2 cut(s) 156, 501
HincII GTYRAC 4 cut(s) 289, 769, 856, 1014
HindII GTYRAC 4 cut(s) 289, 769, 856, 1014
HpaI GTTAAC 1 cut(s) 769
HpaII CCGG 6 cut(s) 125, 295, 314, 692, 902, 1130
HphI GGTGA 4 cut(s) 835, 850, 905, 1117
Hpy166II GTNNAC 6 cut(s) 211, 289, 769, 856, 1014, 1075
Hpy188I TCNGA 6 cut(s) 27, 219, 732, 738, 1000, 1157
Hpy188III TCNNGA 2 cut(s) 692, 1072
Hpy8I GTNNAC 6 cut(s) 211, 289, 769, 856, 1014, 1075
HpyAV CCTTC 4 cut(s) 384, 811, 919, 1027
HpyCH4III ACNGT 3 cut(s) 717, 742, 1089
HpyCH4V TGCA 5 cut(s) 494, 966, 1044, 1093, 1138
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 277, 445, 500, 504, 1135
HpyF3I CTNAG 1 cut(s) 216
Hsp92I GRCGYC 5 cut(s) 78, 285, 399, 852, 1068
Hsp92II CATG 3 cut(s) 367, 901, 970
HspAI GCGC 2 cut(s) 156, 501
Kpn2I TCCGGA 1 cut(s) 691
KspAI GTTAAC 1 cut(s) 769
KspI CCGCGG 2 cut(s) 606, 633
Kzo9I GATC 2 cut(s) 688, 888
LmnI GCTCC 5 cut(s) 67, 344, 417, 755, 1157
Lsp1109I GCAGC 1 cut(s) 950
MaeI CTAG 1 cut(s) 440
MaeIII GTNAC 4 cut(s) 652, 673, 893, 1105
MalI GATC 2 cut(s) 690, 890
MbiI CCGCTC 3 cut(s) 67, 339, 526
MboI GATC 2 cut(s) 688, 888
MboII GAAGA 5 cut(s) 65, 68, 293, 347, 821
MhlI GDGCHC 2 cut(s) 668, 1162
MluCI AATT 2 cut(s) 380, 1167
MlyI GAGTC 8 cut(s) 95, 307, 346, 569, 593, 704, 727, 869
MmeI TCCRAC 5 cut(s) 755, 761, 846, 1023, 1135
MroI TCCGGA 1 cut(s) 691
MseI TTAA 2 cut(s) 192, 768
MspA1I CMGCKG 2 cut(s) 605, 632
MspI CCGG 6 cut(s) 125, 295, 314, 692, 902, 1130
MspR9I CCNGG 1 cut(s) 199
Mva1269I GAATGC 1 cut(s) 386
MvaI CCWGG 1 cut(s) 199
MvnI CGCG 6 cut(s) 156, 467, 483, 503, 605, 632
MwoI GCNNNNNNNGC 6 cut(s) 11, 277, 445, 500, 504, 1135
NdeII GATC 2 cut(s) 688, 888
NlaIII CATG 3 cut(s) 367, 901, 970
NlaIV GGNNCC 2 cut(s) 152, 419
NmeAIII GCCGAG 1 cut(s) 647
NmuCI GTSAC 4 cut(s) 652, 673, 893, 1105
NspI RCATGY 2 cut(s) 367, 901
PaqCI CACCTGC 1 cut(s) 1035
PcsI WCGNNNNNNNCGW 4 cut(s) 279, 566, 674, 1074
PctI GAATGC 1 cut(s) 386
PfeI GAWTC 2 cut(s) 228, 762
Pfl23II CGTACG 1 cut(s) 717
PflMI CCANNNNNTGG 2 cut(s) 147, 705
PkrI GCNGC 4 cut(s) 500, 607, 631, 965
PleI GAGTC 8 cut(s) 94, 306, 346, 569, 593, 703, 727, 868
PmaCI CACGTG 3 cut(s) 251, 274, 561
PmlI CACGTG 3 cut(s) 251, 274, 561
PpsI GAGTC 8 cut(s) 94, 306, 346, 569, 593, 703, 727, 868
Ppu21I YACGTR 6 cut(s) 251, 274, 561, 570, 652, 678
Psp6I CCWGG 1 cut(s) 197
PspCI CACGTG 3 cut(s) 251, 274, 561
PspGI CCWGG 1 cut(s) 197
PspLI CGTACG 1 cut(s) 717
PspN4I GGNNCC 2 cut(s) 152, 419
PspPI GGNCC 3 cut(s) 475, 713, 1113
RsaI GTAC 5 cut(s) 542, 650, 680, 719, 1149
RsaNI GTAC 5 cut(s) 541, 649, 679, 718, 1148
SacII CCGCGG 2 cut(s) 606, 633
SalI GTCGAC 2 cut(s) 287, 854
SaqAI TTAA 2 cut(s) 192, 768
SatI GCNGC 4 cut(s) 499, 606, 630, 964
Sau3AI GATC 2 cut(s) 688, 888
Sau96I GGNCC 3 cut(s) 475, 713, 1113
SchI GAGTC 8 cut(s) 95, 307, 346, 569, 593, 704, 727, 869
ScrFI CCNGG 1 cut(s) 199
SduI GDGCHC 2 cut(s) 668, 1162
SfcI CTRYAG 1 cut(s) 531
Sfr303I CCGCGG 2 cut(s) 606, 633
SgrBI CCGCGG 2 cut(s) 606, 633
SgrDI CGTCGACG 1 cut(s) 854
SinI GGWCC 1 cut(s) 713
Sse9I AATT 2 cut(s) 380, 1167
SspMI CTAG 1 cut(s) 440
StyD4I CCNGG 1 cut(s) 197
TaaI ACNGT 3 cut(s) 717, 742, 1089
TaqI TCGA 8 cut(s) 282, 288, 350, 396, 411, 687, 855, 950
TasI AATT 2 cut(s) 380, 1167
TatI WGTACW 1 cut(s) 1147
TauI GCSGC 3 cut(s) 501, 608, 632
TfiI GAWTC 2 cut(s) 228, 762
Tru1I TTAA 2 cut(s) 192, 768
Tru9I TTAA 2 cut(s) 192, 768
TscAI CASTG 1 cut(s) 1167
TseFI GTSAC 4 cut(s) 652, 673, 893, 1105
TseI GCWGC 1 cut(s) 963
Tsp45I GTSAC 4 cut(s) 652, 673, 893, 1105
TspDTI ATGAA 1 cut(s) 293
TspGWI ACGGA 5 cut(s) 79, 87, 164, 671, 716
TspRI CASTG 1 cut(s) 1167
Van91I CCANNNNNTGG 2 cut(s) 147, 705
VpaK11BI GGWCC 1 cut(s) 713
XagI CCTNNNNNAGG 1 cut(s) 1022
XapI RAATTY 1 cut(s) 380
XceI RCATGY 2 cut(s) 367, 901
XmiI GTMKAC 2 cut(s) 288, 855
XspI CTAG 1 cut(s) 440
ZraI GACGTC 4 cut(s) 79, 286, 853, 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.