AT1G23890

NHL repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006622
Physical Location & Seq
Reverse (-)
8438766 .. 8440866
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G23890.2

Sequence Viewer

Length: 786 bp
ATGGCTTGGGGATTTTCGTCCGTGTCTCTCTTCTTGTTCGTCGTTTTCAATCTGGTGAGTGGTAAGATAGTTTTAGAAGAAGGGTACGAAGTCACAACGGTGGTCGATGGCCATAAGTCTGGTCTGAATCCATACACGATTCATGCTTTACCAGGATCTTCTAATCTGATCGTCCTTGATTCTTCCGGCAGTACATTTTACACTACTTCCTTTCCTTTGTCCGTTGATAGTGTGATAAACCGGTTTGCTGGGGATGGGAGTTCGGGTCATGTAGATGGTAAAGCTGGTAACAGCCGGTTCAGCAAACCCCGTGGTTTTGCTGTGGATGCTAAAGGGAATGTTTATGTTGCTGATAAGAGTAATAAAGCTATACGGAAGATCAGCTCTTCAGGTTCTGTAACCACCATTGCTGGAGGGATCTCAAAGGCATTTGGACACAGAGATGGACCTGCACAGAATGCTACTTTCTCTAGCGATTTCGAGATAACTTTTGTTCCTCAAAGATGTTGTTTGCTTGTTTCTGATCATGGAAATGAAATGATTCGCCAGATAAATCTTAAGGAGGAGGATTGCCTCGAAAACTCTCATTCTAATTTGGGGACATACTCTCTTTGGTCAATAGGGATTGTCCTTTCCTGCATTCTCGGGGTAGCCATTGGATTTGCTGTTCGCCCTTACGTCATCCGTCATACAGGAAGAAGTGAACCACCTCTCGTTCATCATGACATGGAAGCTTCTCCTAACCAAATTGGGGGAACAAGTTCTGACCTTCTTCTCCTACATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

261

Amino Acids

27.87

Weight (kDa)

6.35

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TEN_NHL PF25021 92 - 139 2.4e-06 Teneurin NHL domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 457
AclWI GGATC 2 cut(s) 163, 425
AcoI YGGCCR 1 cut(s) 109
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 2 cut(s) 86, 193
AfiI CCNNNNNNNGG 1 cut(s) 751
AflII CTTAAG 1 cut(s) 557
AgeI ACCGGT 1 cut(s) 240
AgsI TTSAA 1 cut(s) 49
AjnI CCWGG 1 cut(s) 151
AleI CACNNNNGTG 1 cut(s) 98
AloI GAACNNNNNNTCC 2 cut(s) 651, 683
AluBI AGCT 4 cut(s) 284, 368, 384, 734
AluI AGCT 4 cut(s) 284, 368, 384, 734
Alw26I GTCTC 1 cut(s) 30
AlwI GGATC 2 cut(s) 163, 425
AlwNI CAGNNNCTG 1 cut(s) 395
Ama87I CYCGRG 1 cut(s) 644
AoxI GGCC 1 cut(s) 109
AsiGI ACCGGT 1 cut(s) 240
Asp700I GAANNNNTTC 2 cut(s) 540, 760
AspS9I GGNCC 1 cut(s) 446
AsuHPI GGTGA 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 644
AvaII GGWCC 1 cut(s) 446
BalI TGGCCA 1 cut(s) 111
BccI CCATC 4 cut(s) 101, 248, 269, 437
BciT130I CCWGG 1 cut(s) 153
BclI TGATCA 1 cut(s) 523
BcoDI GTCTC 1 cut(s) 30
BfaI CTAG 1 cut(s) 471
BfrI CTTAAG 1 cut(s) 557
BfuAI ACCTGC 1 cut(s) 457
Bme1390I CCNGG 1 cut(s) 153
Bme18I GGWCC 1 cut(s) 446
BmeT110I CYCGRG 1 cut(s) 644
BmgT120I GGNCC 1 cut(s) 446
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 1 cut(s) 316
BpmI CTGGAG 1 cut(s) 432
BsaJI CCNNGG 1 cut(s) 310
BsaWI WCCGGW 1 cut(s) 240
BsaXI ACNNNNNCTCC 2 cut(s) 250, 280
Bsc4I CCNNNNNNNGG 1 cut(s) 751
Bse118I RCCGGY 2 cut(s) 240, 294
Bse3DI GCAATG 1 cut(s) 405
BseBI CCWGG 1 cut(s) 153
BseDI CCNNGG 1 cut(s) 310
BseGI GGATG 3 cut(s) 259, 331, 681
BseLI CCNNNNNNNGG 1 cut(s) 751
BseMI GCAATG 1 cut(s) 405
BseRI GAGGAG 1 cut(s) 578
BseYI CCCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 435
BshFI GGCC 1 cut(s) 111
BshTI ACCGGT 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 644
BsiSI CCGG 3 cut(s) 186, 241, 295
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 1 cut(s) 751
BsmAI GTCTC 1 cut(s) 30
BsmFI GGGAC 1 cut(s) 613
BsmI GAATGC 2 cut(s) 463, 639
BsnI GGCC 1 cut(s) 111
BsoBI CYCGRG 1 cut(s) 644
Bsp143I GATC 5 cut(s) 155, 168, 378, 417, 523
BspANI GGCC 1 cut(s) 111
BspHI TCATGA 1 cut(s) 721
BspMI ACCTGC 1 cut(s) 457
BspPI GGATC 2 cut(s) 163, 425
BspQI GCTCTTC 1 cut(s) 391
BspTI CTTAAG 1 cut(s) 557
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 2 cut(s) 240, 294
BssAI RCCGGY 2 cut(s) 240, 294
BssECI CCNNGG 1 cut(s) 310
BssMI GATC 5 cut(s) 155, 168, 378, 417, 523
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 1 cut(s) 100
Bst6I CTCTTC 2 cut(s) 35, 391
BstAFI CTTAAG 1 cut(s) 557
BstAPI GCANNNNNTGC 1 cut(s) 458
BstDSI CCRYGG 1 cut(s) 310
BstF5I GGATG 3 cut(s) 259, 331, 681
BstKTI GATC 5 cut(s) 158, 171, 381, 420, 526
BstMAI GTCTC 1 cut(s) 30
BstMBI GATC 5 cut(s) 155, 168, 378, 417, 523
BstMWI GCNNNNNNNGC 3 cut(s) 300, 326, 458
BstNI CCWGG 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 151
BstX2I RGATCY 2 cut(s) 155, 417
BstXI CCANNNNNNTGG 1 cut(s) 119
BstYI RGATCY 2 cut(s) 155, 417
BsuRI GGCC 1 cut(s) 111
BtgI CCRYGG 1 cut(s) 310
BtsCI GGATG 3 cut(s) 259, 331, 681
BveI ACCTGC 1 cut(s) 457
CaiI CAGNNNCTG 1 cut(s) 395
CciI TCATGA 1 cut(s) 721
Cfr10I RCCGGY 2 cut(s) 240, 294
Cfr13I GGNCC 1 cut(s) 446
Csp6I GTAC 2 cut(s) 85, 192
CspAI ACCGGT 1 cut(s) 240
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviAII CATG 5 cut(s) 143, 269, 527, 722, 727
CviJI RGCY 8 cut(s) 5, 111, 284, 294, 368, 384, 653, 734
CviKI_1 RGCY 8 cut(s) 5, 111, 284, 294, 368, 384, 653, 734
CviQI GTAC 2 cut(s) 85, 192
DpnI GATC 5 cut(s) 157, 170, 380, 419, 525
DpnII GATC 5 cut(s) 155, 168, 378, 417, 523
EaeI YGGCCR 1 cut(s) 109
Eam1104I CTCTTC 2 cut(s) 35, 391
EarI CTCTTC 2 cut(s) 35, 391
Eco47I GGWCC 1 cut(s) 446
Eco57I CTGAAG 1 cut(s) 372
Eco88I CYCGRG 1 cut(s) 644
EcoRII CCWGG 1 cut(s) 151
FaeI CATG 5 cut(s) 146, 272, 530, 725, 730
FaqI GGGAC 1 cut(s) 613
FatI CATG 5 cut(s) 142, 268, 526, 721, 726
FbaI TGATCA 1 cut(s) 523
FokI GGATG 3 cut(s) 266, 338, 668
FspBI CTAG 1 cut(s) 471
GsaI CCCAGC 1 cut(s) 252
GsuI CTGGAG 1 cut(s) 432
HaeIII GGCC 1 cut(s) 111
HapII CCGG 3 cut(s) 186, 241, 295
Hin1II CATG 5 cut(s) 146, 272, 530, 725, 730
HindIII AAGCTT 1 cut(s) 732
HinfI GANTC 4 cut(s) 127, 139, 179, 541
HpaII CCGG 3 cut(s) 186, 241, 295
HphI GGTGA 1 cut(s) 67
Hpy166II GTNNAC 1 cut(s) 704
Hpy188I TCNGA 4 cut(s) 126, 168, 523, 766
Hpy188III TCNNGA 2 cut(s) 481, 722
Hpy8I GTNNAC 1 cut(s) 704
Hpy99I CGWCG 1 cut(s) 44
HpyAV CCTTC 2 cut(s) 74, 779
HpyCH4III ACNGT 1 cut(s) 100
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 2 cut(s) 452, 639
HpyF10VI GCNNNNNNNGC 3 cut(s) 300, 326, 458
HpySE526I ACGT 1 cut(s) 678
Hsp92II CATG 5 cut(s) 146, 272, 530, 725, 730
Ksp22I TGATCA 1 cut(s) 523
Kzo9I GATC 5 cut(s) 155, 168, 378, 417, 523
LguI GCTCTTC 1 cut(s) 391
LweI GCATC 1 cut(s) 316
MaeI CTAG 1 cut(s) 471
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 3 cut(s) 91, 287, 397
MalI GATC 5 cut(s) 157, 170, 380, 419, 525
MboI GATC 5 cut(s) 155, 168, 378, 417, 523
MboII GAAGA 8 cut(s) 22, 89, 150, 174, 378, 388, 708, 764
MflI RGATCY 2 cut(s) 155, 417
MlsI TGGCCA 1 cut(s) 111
MluCI AATT 2 cut(s) 592, 747
MluNI TGGCCA 1 cut(s) 111
MnlI CCTC 6 cut(s) 407, 507, 556, 559, 584, 720
Mox20I TGGCCA 1 cut(s) 111
MroXI GAANNNNTTC 2 cut(s) 540, 760
MscI TGGCCA 1 cut(s) 111
MseI TTAA 1 cut(s) 558
MslI CAYNNNNRTG 4 cut(s) 98, 273, 441, 531
Msp20I TGGCCA 1 cut(s) 111
MspCI CTTAAG 1 cut(s) 557
MspI CCGG 3 cut(s) 186, 241, 295
MspR9I CCNGG 1 cut(s) 153
Mva1269I GAATGC 2 cut(s) 463, 639
MvaI CCWGG 1 cut(s) 153
MwoI GCNNNNNNNGC 3 cut(s) 300, 326, 458
NdeII GATC 5 cut(s) 155, 168, 378, 417, 523
NlaIII CATG 5 cut(s) 146, 272, 530, 725, 730
NmuCI GTSAC 1 cut(s) 91
OliI CACNNNNGTG 1 cut(s) 98
PagI TCATGA 1 cut(s) 721
PciSI GCTCTTC 1 cut(s) 391
PctI GAATGC 2 cut(s) 463, 639
PdmI GAANNNNTTC 2 cut(s) 540, 760
PfeI GAWTC 4 cut(s) 127, 139, 179, 541
PinAI ACCGGT 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 151
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 1 cut(s) 151
PspPI GGNCC 1 cut(s) 446
PstNI CAGNNNCTG 1 cut(s) 395
PsuI RGATCY 2 cut(s) 155, 417
RsaI GTAC 2 cut(s) 86, 193
RsaNI GTAC 2 cut(s) 85, 192
RseI CAYNNNNRTG 4 cut(s) 98, 273, 441, 531
SapI GCTCTTC 1 cut(s) 391
SaqAI TTAA 1 cut(s) 558
Sau3AI GATC 5 cut(s) 155, 168, 378, 417, 523
Sau96I GGNCC 1 cut(s) 446
ScrFI CCNGG 1 cut(s) 153
SetI ASST 9 cut(s) 286, 370, 386, 394, 451, 681, 712, 736, 771
SfaNI GCATC 1 cut(s) 316
SinI GGWCC 1 cut(s) 446
SmiMI CAYNNNNRTG 4 cut(s) 98, 273, 441, 531
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 2 cut(s) 592, 747
SspMI CTAG 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 100
TaiI ACGT 1 cut(s) 681
TaqI TCGA 3 cut(s) 105, 480, 576
TasI AATT 2 cut(s) 592, 747
TatI WGTACW 1 cut(s) 191
TfiI GAWTC 4 cut(s) 127, 139, 179, 541
Tru1I TTAA 1 cut(s) 558
Tru9I TTAA 1 cut(s) 558
TseFI GTSAC 1 cut(s) 91
Tsp45I GTSAC 1 cut(s) 91
TspDTI ATGAA 3 cut(s) 131, 549, 707
TspGWI ACGGA 4 cut(s) 10, 211, 388, 674
Vha464I CTTAAG 1 cut(s) 557
VpaK11BI GGWCC 1 cut(s) 446
XmnI GAANNNNTTC 2 cut(s) 540, 760
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.