FvH4_4g34820

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006622
Physical Location & Seq
Reverse (-)
32378210 .. 32380348
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34820.t2

Sequence Viewer

Length: 1149 bp
ATGGCGTCACTTTCCCTTGCTGTCTGCGTCTTCTTCGTCCTCAACCTTGCTTCGCTTCAAGCTTTCGCCGGAGTGGTGCTGGAAGAGGGCTACACAGTCACGACACTGATCGACGGCCACAAACTGGATATAAATCCTTACTCGGTGCTCCCTCGGCCAGGATCGTCCGATCTTCTCGTTCTCGATTCTTCCGAGAATGTGGTAAAGAAGCTCTCCGGCGCCGGCGGCGAGGGTTATTCGGACGGAGAGTCGGTTTTGGCGCGGTTTAGGAAGCCCAGAGGCTTTGCGGTGGGCCAGAAAGGAACTGTGTTTGTTGCGGATAGGAGTAATAATGTGATAAGGAAGATAAGCGCCTCAGGTGTAAGCACTATTGCTGGAGGCTACTCACTGAAGCCTGGGCATGAGGACGGTCCTGCGCAAAACGCAACGTTTAGTTCGGATTTCGACCTGGCTTTTGATGCGGGTAGGTGCGCGCTTTTGGTCTCTGATCGTGGGAATCAGTTGGTGCGGTTGATTAGTCTCAAACCGGAGGACTGTGCCAGGGGTTCTGCACCGTCTGCGCTTGGATCAGTTTCAGTGTGGATTATGGGTCTGGGATTGTGTTTGTTGGGGATTCTAATTGGATTTGTAGCTCGGCATTTCATGCCTTATGAGGGCAGCAGCCAGCTCGGCTTCAGCGTGACATGGAAGCGATGCCAAATCAGTCTGGGGAAGCAAGCACAGACTTTTTGCTTCGCCATCAGAAGCGCAACTGCTAGCTCCTCCACTCCTGTTTTGTCGCTGTTGAGGCGGCTCTTTTGGTTGTGTGTCTCCCAAATTTCTCTTATGTATAGTATTAGCAGTGTGGAGTCCCGAGTTTCGAGTAAGGAAGGTGTATCTTTGCTTGATCTCGATGTCAATAACAGTAGTTGTTCTACGATAACCGAGTCGTCCAAGTATGTGGATCAGTTAAAGGATTTGATGCTTTTAGATGGAAGCACGGAGTTGTTTAGTGCTGACAACCTGATGCTGAAGCAAGATGGTAATGCTGGAAGGAGTGACGTATTATCTGGTTGTCATGGAGGAATTGATGGCATGATTGACAGTAATATTATGGGATTTGTGGACGGAGCTTCGGGAGGTAGTTCAGGTTTAGTTAAGAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

383

Amino Acids

40.36

Weight (kDa)

6.44

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 417
AccB1I GGYRCC 1 cut(s) 218
AccB7I CCANNNNNTGG 1 cut(s) 124
AccII CGCG 2 cut(s) 262, 473
AciI CCGC 7 cut(s) 225, 262, 287, 317, 461, 508, 790
AclI AACGTT 1 cut(s) 428
AclWI GGATC 3 cut(s) 169, 574, 951
AcoI YGGCCR 2 cut(s) 115, 155
AcsI RAATTY 1 cut(s) 816
AcuI CTGAAG 3 cut(s) 410, 658, 1031
AcyI GRCGYC 2 cut(s) 5, 219
AfiI CCNNNNNNNGG 3 cut(s) 124, 158, 653
AgsI TTSAA 1 cut(s) 59
AhdI GACNNNNNGTC 1 cut(s) 247
AjnI CCWGG 4 cut(s) 157, 394, 447, 539
AluBI AGCT 6 cut(s) 62, 211, 632, 667, 759, 1112
AluI AGCT 6 cut(s) 62, 211, 632, 667, 759, 1112
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 3 cut(s) 487, 524, 814
AlwI GGATC 3 cut(s) 169, 574, 951
Ama87I CYCGRG 1 cut(s) 852
AoxI GGCC 3 cut(s) 115, 155, 292
ApeKI GCWGC 2 cut(s) 657, 660
ApoI RAATTY 1 cut(s) 816
ArsI GACNNNNNNTTYG 2 cut(s) 437, 469
AspLEI GCGC 8 cut(s) 221, 262, 353, 418, 473, 475, 562, 749
AspS9I GGNCC 2 cut(s) 292, 410
AsuNHI GCTAGC 1 cut(s) 755
AvaI CYCGRG 1 cut(s) 852
AvaII GGWCC 1 cut(s) 410
AxyI CCTNAGG 1 cut(s) 355
BanI GGYRCC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 22
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 2 cut(s) 669, 672
BccI CCATC 4 cut(s) 746, 965, 1013, 1064
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 2 cut(s) 649, 683
BciT130I CCWGG 4 cut(s) 159, 396, 449, 541
BcoDI GTCTC 3 cut(s) 487, 524, 814
BfaI CTAG 1 cut(s) 756
BfoI RGCGCY 2 cut(s) 222, 354
BglI GCCNNNNNGGC 1 cut(s) 669
BisI GCNGC 4 cut(s) 226, 658, 661, 791
BlsI GCNGC 4 cut(s) 227, 659, 662, 792
Bme1390I CCNGG 4 cut(s) 159, 396, 449, 541
Bme18I GGWCC 1 cut(s) 410
BmeRI GACNNNNNGTC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 852
BmgT120I GGNCC 2 cut(s) 292, 410
BmiI GGNNCC 1 cut(s) 220
BmrFI CCNGG 4 cut(s) 159, 396, 449, 541
BmsI GCATC 4 cut(s) 448, 683, 951, 996
BmtI GCTAGC 1 cut(s) 759
BpiI GAAGAC 1 cut(s) 22
BpmI CTGGAG 1 cut(s) 396
BsaBI GATNNNNATC 1 cut(s) 132
BsaHI GRCGYC 2 cut(s) 5, 219
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 3 cut(s) 152, 395, 540
BsaWI WCCGGW 1 cut(s) 526
BsaXI ACNNNNNCTCC 2 cut(s) 237, 267
Bsc4I CCNNNNNNNGG 3 cut(s) 124, 158, 653
Bse118I RCCGGY 1 cut(s) 221
Bse1I ACTGG 1 cut(s) 129
Bse21I CCTNAGG 1 cut(s) 355
Bse8I GATNNNNATC 1 cut(s) 132
BseBI CCWGG 4 cut(s) 159, 396, 449, 541
BseDI CCNNGG 3 cut(s) 152, 395, 540
BseJI GATNNNNATC 1 cut(s) 132
BseLI CCNNNNNNNGG 3 cut(s) 124, 158, 653
BseMII CTCAG 1 cut(s) 369
BseNI ACTGG 1 cut(s) 129
BsePI GCGCGC 1 cut(s) 471
BseRI GAGGAG 1 cut(s) 751
BseXI GCAGC 2 cut(s) 669, 672
BsgI GTGCAG 1 cut(s) 534
Bsh1236I CGCG 2 cut(s) 262, 473
BshFI GGCC 3 cut(s) 117, 157, 294
BshNI GGYRCC 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 1 cut(s) 852
BsiSI CCGG 4 cut(s) 69, 216, 222, 527
BslFI GGGAC 1 cut(s) 835
BslI CCNNNNNNNGG 3 cut(s) 124, 158, 653
BsmAI GTCTC 3 cut(s) 487, 524, 814
BsmFI GGGAC 1 cut(s) 835
BsnI GGCC 3 cut(s) 117, 157, 294
Bso31I GGTCTC 1 cut(s) 487
BsoBI CYCGRG 1 cut(s) 852
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 7 cut(s) 108, 161, 169, 487, 566, 886, 943
BspACI CCGC 7 cut(s) 225, 262, 287, 317, 461, 508, 790
BspANI GGCC 3 cut(s) 117, 157, 294
BspCNI CTCAG 1 cut(s) 368
BspFNI CGCG 2 cut(s) 262, 473
BspLI GGNNCC 1 cut(s) 220
BspOI GCTAGC 1 cut(s) 759
BspPI GGATC 3 cut(s) 169, 574, 951
BspT107I GGYRCC 1 cut(s) 218
BspTNI GGTCTC 1 cut(s) 487
BsrFI RCCGGY 1 cut(s) 221
BsrI ACTGG 1 cut(s) 129
BssAI RCCGGY 1 cut(s) 221
BssECI CCNNGG 3 cut(s) 152, 395, 540
BssHII GCGCGC 1 cut(s) 471
BssMI GATC 7 cut(s) 108, 161, 169, 487, 566, 886, 943
BssNI GRCGYC 2 cut(s) 5, 219
Bst2UI CCWGG 4 cut(s) 159, 396, 449, 541
Bst4CI ACNGT 7 cut(s) 97, 307, 410, 536, 555, 905, 1085
Bst6I CTCTTC 1 cut(s) 78
BstACI GRCGYC 2 cut(s) 5, 219
BstAPI GCANNNNNTGC 2 cut(s) 557, 643
BstC8I GCNNGC 5 cut(s) 223, 473, 665, 717, 757
BstDEI CTNAG 1 cut(s) 355
BstENI CCTNNNNNAGG 1 cut(s) 156
BstFNI CGCG 2 cut(s) 262, 473
BstH2I RGCGCY 2 cut(s) 222, 354
BstHHI GCGC 8 cut(s) 221, 262, 353, 418, 473, 475, 562, 749
BstKTI GATC 7 cut(s) 111, 164, 172, 490, 569, 889, 946
BstMAI GTCTC 3 cut(s) 487, 524, 814
BstMBI GATC 7 cut(s) 108, 161, 169, 487, 566, 886, 943
BstMWI GCNNNNNNNGC 8 cut(s) 154, 225, 422, 458, 557, 643, 669, 787
BstNI CCWGG 4 cut(s) 159, 396, 449, 541
BstSCI CCNGG 4 cut(s) 157, 394, 447, 539
BstUI CGCG 2 cut(s) 262, 473
BstV1I GCAGC 2 cut(s) 669, 672
BstV2I GAAGAC 1 cut(s) 22
BstXI CCANNNNNNTGG 1 cut(s) 940
Bsu36I CCTNAGG 1 cut(s) 355
BsuRI GGCC 3 cut(s) 117, 157, 294
BtgZI GCGATG 1 cut(s) 706
BtsI GCAGTG 1 cut(s) 847
BtsIMutI CAGTG 4 cut(s) 104, 386, 582, 847
Cac8I GCNNGC 5 cut(s) 223, 473, 665, 717, 757
CfoI GCGC 8 cut(s) 221, 262, 353, 418, 473, 475, 562, 749
Cfr10I RCCGGY 1 cut(s) 221
Cfr13I GGNCC 2 cut(s) 292, 410
CseI GACGC 1 cut(s) 16
CviAII CATG 5 cut(s) 401, 643, 684, 1058, 1075
DdeI CTNAG 1 cut(s) 355
DinI GGCGCC 1 cut(s) 220
DpnI GATC 7 cut(s) 110, 163, 171, 489, 568, 888, 945
DpnII GATC 7 cut(s) 108, 161, 169, 487, 566, 886, 943
DriI GACNNNNNGTC 1 cut(s) 247
EaeI YGGCCR 2 cut(s) 115, 155
Eam1104I CTCTTC 1 cut(s) 78
Eam1105I GACNNNNNGTC 1 cut(s) 247
EarI CTCTTC 1 cut(s) 78
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 410
Eco57I CTGAAG 3 cut(s) 410, 658, 1031
Eco81I CCTNAGG 1 cut(s) 355
Eco88I CYCGRG 1 cut(s) 852
EcoNI CCTNNNNNAGG 1 cut(s) 156
EcoRII CCWGG 4 cut(s) 157, 394, 447, 539
EgeI GGCGCC 1 cut(s) 220
EheI GGCGCC 1 cut(s) 220
FaeI CATG 5 cut(s) 404, 646, 687, 1061, 1078
FaqI GGGAC 1 cut(s) 835
FatI CATG 5 cut(s) 400, 642, 683, 1057, 1074
FauI CCCGC 1 cut(s) 454
Fnu4HI GCNGC 4 cut(s) 226, 658, 661, 791
Fsp4HI GCNGC 4 cut(s) 226, 658, 661, 791
FspBI CTAG 1 cut(s) 756
FspI TGCGCA 1 cut(s) 417
GlaI GCGC 8 cut(s) 220, 261, 352, 417, 472, 474, 561, 748
GluI GCNGC 4 cut(s) 226, 658, 661, 791
GsuI CTGGAG 1 cut(s) 396
HaeII RGCGCY 2 cut(s) 222, 354
HaeIII GGCC 3 cut(s) 117, 157, 294
HapII CCGG 4 cut(s) 69, 216, 222, 527
HgaI GACGC 1 cut(s) 16
HhaI GCGC 8 cut(s) 221, 262, 353, 418, 473, 475, 562, 749
Hin1I GRCGYC 2 cut(s) 5, 219
Hin1II CATG 5 cut(s) 404, 646, 687, 1061, 1078
Hin6I GCGC 8 cut(s) 219, 260, 351, 416, 471, 473, 560, 747
HinP1I GCGC 8 cut(s) 219, 260, 351, 416, 471, 473, 560, 747
HindIII AAGCTT 1 cut(s) 60
HinfI GANTC 6 cut(s) 185, 248, 496, 613, 848, 926
HpaII CCGG 4 cut(s) 69, 216, 222, 527
Hpy166II GTNNAC 1 cut(s) 1105
Hpy188I TCNGA 6 cut(s) 169, 193, 241, 439, 487, 743
Hpy188III TCNNGA 5 cut(s) 100, 182, 852, 890, 1116
Hpy8I GTNNAC 1 cut(s) 1105
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 2 cut(s) 863, 1026
HpyCH4III ACNGT 7 cut(s) 97, 307, 410, 536, 555, 905, 1085
HpyCH4IV ACGT 2 cut(s) 428, 1041
HpyCH4V TGCA 1 cut(s) 551
HpyF10VI GCNNNNNNNGC 8 cut(s) 154, 225, 422, 458, 557, 643, 669, 787
HpyF3I CTNAG 1 cut(s) 355
HpySE526I ACGT 2 cut(s) 428, 1041
Hsp92I GRCGYC 2 cut(s) 5, 219
Hsp92II CATG 5 cut(s) 404, 646, 687, 1061, 1078
HspAI GCGC 8 cut(s) 219, 260, 351, 416, 471, 473, 560, 747
KasI GGCGCC 1 cut(s) 218
KroI GCCGGC 1 cut(s) 221
KroNI GCCGGC 1 cut(s) 223
Kzo9I GATC 7 cut(s) 108, 161, 169, 487, 566, 886, 943
LmnI GCTCC 3 cut(s) 153, 764, 1109
Lsp1109I GCAGC 2 cut(s) 669, 672
LweI GCATC 4 cut(s) 448, 683, 951, 996
MaeI CTAG 1 cut(s) 756
MaeII ACGT 2 cut(s) 428, 1041
MaeIII GTNAC 4 cut(s) 6, 97, 679, 1037
MalI GATC 7 cut(s) 110, 163, 171, 489, 568, 888, 945
MboI GATC 7 cut(s) 108, 161, 169, 487, 566, 886, 943
MboII GAAGA 6 cut(s) 22, 25, 95, 164, 180, 355
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 3 cut(s) 618, 816, 1065
Mly113I GGCGCC 1 cut(s) 219
MlyI GAGTC 3 cut(s) 257, 857, 935
MreI CGCCGGCG 1 cut(s) 221
MroNI GCCGGC 1 cut(s) 221
MseI TTAA 2 cut(s) 950, 1137
MspI CCGG 4 cut(s) 69, 216, 222, 527
MspR9I CCNGG 4 cut(s) 159, 396, 449, 541
MvaI CCWGG 4 cut(s) 159, 396, 449, 541
MvnI CGCG 2 cut(s) 262, 473
MwoI GCNNNNNNNGC 8 cut(s) 154, 225, 422, 458, 557, 643, 669, 787
NaeI GCCGGC 1 cut(s) 223
NarI GGCGCC 1 cut(s) 219
NdeII GATC 7 cut(s) 108, 161, 169, 487, 566, 886, 943
NgoMIV GCCGGC 1 cut(s) 221
NheI GCTAGC 1 cut(s) 755
NlaIII CATG 5 cut(s) 404, 646, 687, 1061, 1078
NlaIV GGNNCC 1 cut(s) 220
NmeAIII GCCGAG 3 cut(s) 133, 613, 648
NmuCI GTSAC 4 cut(s) 6, 97, 679, 1037
NsbI TGCGCA 1 cut(s) 417
PauI GCGCGC 1 cut(s) 471
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 675
PdiI GCCGGC 1 cut(s) 223
PfeI GAWTC 3 cut(s) 185, 496, 613
PflMI CCANNNNNTGG 1 cut(s) 124
PkrI GCNGC 4 cut(s) 227, 659, 662, 792
PleI GAGTC 3 cut(s) 256, 856, 934
PluTI GGCGCC 1 cut(s) 222
PpsI GAGTC 3 cut(s) 256, 856, 934
Psp1406I AACGTT 1 cut(s) 428
Psp6I CCWGG 4 cut(s) 157, 394, 447, 539
PspGI CCWGG 4 cut(s) 157, 394, 447, 539
PspN4I GGNNCC 1 cut(s) 220
PspPI GGNCC 2 cut(s) 292, 410
PteI GCGCGC 1 cut(s) 471
SaqAI TTAA 2 cut(s) 950, 1137
SatI GCNGC 4 cut(s) 226, 658, 661, 791
Sau3AI GATC 7 cut(s) 108, 161, 169, 487, 566, 886, 943
Sau96I GGNCC 2 cut(s) 292, 410
SchI GAGTC 3 cut(s) 257, 857, 935
ScrFI CCNGG 4 cut(s) 159, 396, 449, 541
SduI GDGCHC 1 cut(s) 150
SfaNI GCATC 4 cut(s) 448, 683, 951, 996
SfoI GGCGCC 1 cut(s) 220
SgrAI CRCCGGYG 1 cut(s) 221
SinI GGWCC 1 cut(s) 410
Sse9I AATT 3 cut(s) 618, 816, 1065
SsiI CCGC 7 cut(s) 225, 262, 287, 317, 461, 508, 790
SspDI GGCGCC 1 cut(s) 218
SspI AATATT 1 cut(s) 1090
SspMI CTAG 1 cut(s) 756
StyD4I CCNGG 4 cut(s) 157, 394, 447, 539
TaaI ACNGT 7 cut(s) 97, 307, 410, 536, 555, 905, 1085
TaiI ACGT 2 cut(s) 431, 1044
TaqI TCGA 5 cut(s) 111, 183, 444, 860, 891
TasI AATT 3 cut(s) 618, 816, 1065
TauI GCSGC 2 cut(s) 228, 793
TfiI GAWTC 3 cut(s) 185, 496, 613
Tru1I TTAA 2 cut(s) 950, 1137
Tru9I TTAA 2 cut(s) 950, 1137
TscAI CASTG 4 cut(s) 111, 393, 582, 847
TseFI GTSAC 4 cut(s) 6, 97, 679, 1037
TseI GCWGC 2 cut(s) 657, 660
Tsp45I GTSAC 4 cut(s) 6, 97, 679, 1037
TspDTI ATGAA 1 cut(s) 631
TspGWI ACGGA 3 cut(s) 258, 995, 1122
TspRI CASTG 4 cut(s) 111, 393, 582, 847
Van91I CCANNNNNTGG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 410
XagI CCTNNNNNAGG 1 cut(s) 156
XapI RAATTY 1 cut(s) 816
XspI CTAG 1 cut(s) 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.