pycom13g07780

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0006622
Physical Location & Seq
Forward (+)
5148063 .. 5149345
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g07780.6

Sequence Viewer

Length: 813 bp
ATGGCGTTGCGCTTCCAGTCCTCCGCCGTTGCTCTCTTCGTCTTCCTCCTCAGCGTCGCCCCACCTCGAGTCTCCGCCGACGTGGTGCTGGAAGACGGGTACACATTCACGACGGTGATCGACGGCCACAAACTCGGCGTGAACCCCTACGCGGTGCTCGCTCCCCCTGGATCGTCCGATCTGCTCTTCCTCGACTCTTCCGGTAGCGCCTTCTACGCCGTACCATTTCCCACGCCCGGATCCCAAGAGAGTGTGATTAAACGGTTATCCGGCAACGGCGACGGATATTCGGATGGGGAGTCGGGTTCGGCCCGGTTCAAGAAGCCCCGGAGCTTCGCCGTGAGTCCGAAAGGAAACGTCTTCGTAGCCGATAAGGGCAATAACGTCATTCGGAAGATAAGCGCTTCAGGTGTGAGCACGATTGCCGGAGGGTACTCGGTGAAGCCTGGGCGCGAGGACGGCCCTGCTCAAAACGCAACGTTTTCACCGGACATTGAGCTCGCTTTTGCTGCCGATCAGTGCGCGCTGCTTGTCTCCGATCGCGGGAATCAGTTGGTCCGGTTGATTAATCTCAAGCCGGAGGACTGCGCCAGGAGTTATCCGTCCAGTGAAATTTGGAGCAGCTTCGATTTGGATTTTGGGTCTCGGCTTGTCGTGTGTTTTCGGAATTGTTGTTGGATTTGTGATGCGGCCTTACATATTCTCCAATGTAAGTCGGCTCGTCTCCCGCACGACATGGAAGCGCTGCTGAATCATTCTGGGGAAGCAAGTAGTACAGACACTTTGCTTCGCCGTCAGAGGCTCAACTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NHL

Protein Analysis

271

Amino Acids

28.93

Weight (kDa)

6.42

Isoelectric Point (pI)

45.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 152, 453, 524, 543
AciI CCGC 6 cut(s) 24, 75, 152, 543, 689, 728
AclI AACGTT 1 cut(s) 479
AclWI GGATC 3 cut(s) 178, 234, 247
AcoI YGGCCR 1 cut(s) 124
AcsI RAATTY 1 cut(s) 612
AcuI CTGAAG 1 cut(s) 390
AfaI GTAC 4 cut(s) 101, 222, 434, 775
AfeI AGCGCT 2 cut(s) 403, 744
AfiI CCNNNNNNNGG 3 cut(s) 151, 236, 543
AgsI TTSAA 1 cut(s) 319
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 3 cut(s) 166, 445, 590
AleI CACNNNNGTG 1 cut(s) 113
AluBI AGCT 3 cut(s) 333, 499, 624
AluI AGCT 3 cut(s) 333, 499, 624
Alw21I GWGCWC 3 cut(s) 159, 419, 501
Alw26I GTCTC 4 cut(s) 76, 538, 648, 728
AlwI GGATC 3 cut(s) 178, 234, 247
Ama87I CYCGRG 1 cut(s) 66
Aor51HI AGCGCT 2 cut(s) 403, 744
AoxI GGCC 4 cut(s) 124, 309, 460, 690
ApeKI GCWGC 4 cut(s) 509, 526, 621, 745
ApoI RAATTY 1 cut(s) 612
AseI ATTAAT 1 cut(s) 567
AspLEI GCGC 8 cut(s) 12, 209, 404, 453, 524, 526, 590, 745
AspS9I GGNCC 3 cut(s) 310, 461, 556
AsuC2I CCSGG 3 cut(s) 237, 313, 328
AsuHPI GGTGA 3 cut(s) 127, 451, 477
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 1 cut(s) 556
BamHI GGATCC 1 cut(s) 239
BanII GRGCYC 1 cut(s) 501
BbsI GAAGAC 3 cut(s) 34, 99, 352
Bbv12I GWGCWC 3 cut(s) 159, 419, 501
BbvCI CCTCAGC 1 cut(s) 50
BbvI GCAGC 4 cut(s) 496, 513, 633, 732
BccI CCATC 1 cut(s) 287
BceAI ACGGC 7 cut(s) 11, 139, 203, 292, 323, 475, 777
BciT130I CCWGG 3 cut(s) 168, 447, 592
BcnI CCSGG 3 cut(s) 237, 313, 328
BcoDI GTCTC 4 cut(s) 76, 538, 648, 728
BfaI CTAG 1 cut(s) 811
BfoI RGCGCY 3 cut(s) 210, 405, 746
BisI GCNGC 5 cut(s) 510, 527, 622, 690, 746
BlsI GCNGC 5 cut(s) 511, 528, 623, 691, 747
Bme1390I CCNGG 6 cut(s) 168, 237, 313, 328, 447, 592
Bme18I GGWCC 1 cut(s) 556
BmeT110I CYCGRG 1 cut(s) 66
BmgBI CACGTC 1 cut(s) 82
BmgT120I GGNCC 3 cut(s) 310, 461, 556
BmiI GGNNCC 1 cut(s) 241
BmrFI CCNGG 6 cut(s) 168, 237, 313, 328, 447, 592
BmsI GCATC 1 cut(s) 676
BpiI GAAGAC 3 cut(s) 34, 99, 352
Bpu10I CCTNAGC 1 cut(s) 50
BpuEI CTTGAG 1 cut(s) 557
BpuMI CCSGG 3 cut(s) 237, 313, 328
BsaI GGTCTC 1 cut(s) 648
BsaJI CCNNGG 3 cut(s) 166, 326, 446
BsaWI WCCGGW 3 cut(s) 200, 487, 558
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 236, 543
Bse1I ACTGG 2 cut(s) 16, 606
BseBI CCWGG 3 cut(s) 168, 447, 592
BseDI CCNNGG 3 cut(s) 166, 326, 446
BseGI GGATG 1 cut(s) 298
BseLI CCNNNNNNNGG 3 cut(s) 151, 236, 543
BseMII CTCAG 1 cut(s) 64
BseNI ACTGG 2 cut(s) 16, 606
BsePI GCGCGC 1 cut(s) 522
BseRI GAGGAG 1 cut(s) 38
BseXI GCAGC 4 cut(s) 496, 513, 633, 732
Bsh1236I CGCG 4 cut(s) 152, 453, 524, 543
Bsh1285I CGRYCG 1 cut(s) 541
BshFI GGCC 4 cut(s) 126, 311, 462, 692
BsiEI CGRYCG 1 cut(s) 541
BsiHKAI GWGCWC 3 cut(s) 159, 419, 501
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 9 cut(s) 201, 237, 270, 313, 328, 426, 488, 559, 578
BslI CCNNNNNNNGG 3 cut(s) 151, 236, 543
BsmAI GTCTC 4 cut(s) 76, 538, 648, 728
BsmBI CGTCTC 1 cut(s) 728
BsnI GGCC 4 cut(s) 126, 311, 462, 692
Bso31I GGTCTC 1 cut(s) 648
BsoBI CYCGRG 1 cut(s) 66
Bsp1286I GDGCHC 3 cut(s) 159, 419, 501
Bsp143I GATC 6 cut(s) 117, 170, 178, 239, 514, 538
BspACI CCGC 6 cut(s) 24, 75, 152, 543, 689, 728
BspANI GGCC 4 cut(s) 126, 311, 462, 692
BspCNI CTCAG 1 cut(s) 63
BspFNI CGCG 4 cut(s) 152, 453, 524, 543
BspLI GGNNCC 1 cut(s) 241
BspPI GGATC 3 cut(s) 178, 234, 247
BspQI GCTCTTC 1 cut(s) 191
BspTNI GGTCTC 1 cut(s) 648
BsrI ACTGG 2 cut(s) 16, 606
BssECI CCNNGG 3 cut(s) 166, 326, 446
BssHII GCGCGC 1 cut(s) 522
BssMI GATC 6 cut(s) 117, 170, 178, 239, 514, 538
Bst2UI CCWGG 3 cut(s) 168, 447, 592
Bst4CI ACNGT 2 cut(s) 115, 264
Bst6I CTCTTC 3 cut(s) 41, 191, 202
BstC8I GCNNGC 3 cut(s) 159, 501, 524
BstDEI CTNAG 1 cut(s) 50
BstF5I GGATG 1 cut(s) 298
BstFNI CGCG 4 cut(s) 152, 453, 524, 543
BstH2I RGCGCY 3 cut(s) 210, 405, 746
BstHHI GCGC 8 cut(s) 12, 209, 404, 453, 524, 526, 590, 745
BstKTI GATC 6 cut(s) 120, 173, 181, 242, 517, 541
BstMAI GTCTC 4 cut(s) 76, 538, 648, 728
BstMBI GATC 6 cut(s) 117, 170, 178, 239, 514, 538
BstMCI CGRYCG 1 cut(s) 541
BstMWI GCNNNNNNNGC 5 cut(s) 158, 215, 459, 473, 509
BstNI CCWGG 3 cut(s) 168, 447, 592
BstSCI CCNGG 6 cut(s) 166, 235, 311, 326, 445, 590
BstUI CGCG 4 cut(s) 152, 453, 524, 543
BstV1I GCAGC 4 cut(s) 496, 513, 633, 732
BstV2I GAAGAC 3 cut(s) 34, 99, 352
BstX2I RGATCY 1 cut(s) 239
BstYI RGATCY 1 cut(s) 239
BsuRI GGCC 4 cut(s) 126, 311, 462, 692
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 1 cut(s) 298
BtsIMutI CAGTG 2 cut(s) 524, 613
Cac8I GCNNGC 3 cut(s) 159, 501, 524
CfoI GCGC 8 cut(s) 12, 209, 404, 453, 524, 526, 590, 745
Cfr13I GGNCC 3 cut(s) 310, 461, 556
CseI GACGC 1 cut(s) 43
Csp6I GTAC 4 cut(s) 100, 221, 433, 774
CviAII CATG 1 cut(s) 736
CviQI GTAC 4 cut(s) 100, 221, 433, 774
DdeI CTNAG 1 cut(s) 50
DpnI GATC 6 cut(s) 119, 172, 180, 241, 516, 540
DpnII GATC 6 cut(s) 117, 170, 178, 239, 514, 538
EaeI YGGCCR 1 cut(s) 124
Eam1104I CTCTTC 3 cut(s) 41, 191, 202
EarI CTCTTC 3 cut(s) 41, 191, 202
EciI GGCGGA 2 cut(s) 13, 64
Ecl136II GAGCTC 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 501
Eco31I GGTCTC 1 cut(s) 648
Eco47I GGWCC 1 cut(s) 556
Eco47III AGCGCT 2 cut(s) 403, 744
Eco53kI GAGCTC 1 cut(s) 499
Eco57I CTGAAG 1 cut(s) 390
Eco88I CYCGRG 1 cut(s) 66
EcoICRI GAGCTC 1 cut(s) 499
EcoRII CCWGG 3 cut(s) 166, 445, 590
EcoT38I GRGCYC 1 cut(s) 501
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 1 cut(s) 739
FaiI YATR 2 cut(s) 699, 737
FatI CATG 1 cut(s) 735
FauI CCCGC 2 cut(s) 536, 735
Fnu4HI GCNGC 5 cut(s) 510, 527, 622, 690, 746
FokI GGATG 1 cut(s) 305
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 5 cut(s) 510, 527, 622, 690, 746
FspBI CTAG 1 cut(s) 811
GlaI GCGC 8 cut(s) 11, 208, 403, 452, 523, 525, 589, 744
GluI GCNGC 5 cut(s) 510, 527, 622, 690, 746
HaeII RGCGCY 3 cut(s) 210, 405, 746
HaeIII GGCC 4 cut(s) 126, 311, 462, 692
HapII CCGG 9 cut(s) 201, 237, 270, 313, 328, 426, 488, 559, 578
HgaI GACGC 1 cut(s) 43
HhaI GCGC 8 cut(s) 12, 209, 404, 453, 524, 526, 590, 745
Hin1II CATG 1 cut(s) 739
Hin6I GCGC 8 cut(s) 10, 207, 402, 451, 522, 524, 588, 743
HinP1I GCGC 8 cut(s) 10, 207, 402, 451, 522, 524, 588, 743
HinfI GANTC 6 cut(s) 69, 194, 299, 343, 547, 751
HpaII CCGG 9 cut(s) 201, 237, 270, 313, 328, 426, 488, 559, 578
HphI GGTGA 3 cut(s) 127, 451, 477
Hpy166II GTNNAC 2 cut(s) 102, 142
Hpy188I TCNGA 7 cut(s) 178, 292, 348, 393, 538, 666, 798
Hpy188III TCNNGA 2 cut(s) 109, 319
Hpy8I GTNNAC 2 cut(s) 102, 142
Hpy99I CGWCG 5 cut(s) 59, 83, 115, 125, 284
HpyAV CCTTC 1 cut(s) 220
HpyCH4III ACNGT 2 cut(s) 115, 264
HpyCH4IV ACGT 4 cut(s) 81, 357, 384, 479
HpyF10VI GCNNNNNNNGC 5 cut(s) 158, 215, 459, 473, 509
HpyF3I CTNAG 1 cut(s) 50
HpySE526I ACGT 4 cut(s) 81, 357, 384, 479
Hsp92II CATG 1 cut(s) 739
HspAI GCGC 8 cut(s) 10, 207, 402, 451, 522, 524, 588, 743
Kzo9I GATC 6 cut(s) 117, 170, 178, 239, 514, 538
LguI GCTCTTC 1 cut(s) 191
LmnI GCTCC 3 cut(s) 166, 330, 618
Lsp1109I GCAGC 4 cut(s) 496, 513, 633, 732
LweI GCATC 1 cut(s) 676
MaeI CTAG 1 cut(s) 811
MaeII ACGT 4 cut(s) 81, 357, 384, 479
MalI GATC 6 cut(s) 119, 172, 180, 241, 516, 540
MboI GATC 6 cut(s) 117, 170, 178, 239, 514, 538
MboII GAAGA 7 cut(s) 28, 34, 104, 178, 189, 352, 406
MflI RGATCY 1 cut(s) 239
MhlI GDGCHC 3 cut(s) 159, 419, 501
MluCI AATT 2 cut(s) 612, 667
MlyI GAGTC 4 cut(s) 78, 188, 308, 352
MmeI TCCRAC 1 cut(s) 656
MnlI CCTC 9 cut(s) 31, 56, 59, 75, 200, 422, 448, 574, 792
MseI TTAA 2 cut(s) 258, 567
MslI CAYNNNNRTG 1 cut(s) 113
MspI CCGG 9 cut(s) 201, 237, 270, 313, 328, 426, 488, 559, 578
MspR9I CCNGG 6 cut(s) 168, 237, 313, 328, 447, 592
MvaI CCWGG 3 cut(s) 168, 447, 592
MvnI CGCG 4 cut(s) 152, 453, 524, 543
MwoI GCNNNNNNNGC 5 cut(s) 158, 215, 459, 473, 509
NciI CCSGG 3 cut(s) 237, 313, 328
NdeII GATC 6 cut(s) 117, 170, 178, 239, 514, 538
NlaIII CATG 1 cut(s) 739
NlaIV GGNNCC 1 cut(s) 241
NmeAIII GCCGAG 2 cut(s) 114, 625
OliI CACNNNNGTG 1 cut(s) 113
PaeR7I CTCGAG 1 cut(s) 66
PauI GCGCGC 1 cut(s) 522
PciSI GCTCTTC 1 cut(s) 191
PfeI GAWTC 2 cut(s) 547, 751
PkrI GCNGC 5 cut(s) 511, 528, 623, 691, 747
Ple19I CGATCG 1 cut(s) 541
PleI GAGTC 4 cut(s) 77, 188, 307, 351
PpsI GAGTC 4 cut(s) 77, 188, 307, 351
PshBI ATTAAT 1 cut(s) 567
Psp124BI GAGCTC 1 cut(s) 501
Psp1406I AACGTT 1 cut(s) 479
Psp6I CCWGG 3 cut(s) 166, 445, 590
PspGI CCWGG 3 cut(s) 166, 445, 590
PspN4I GGNNCC 1 cut(s) 241
PspPI GGNCC 3 cut(s) 310, 461, 556
PspXI VCTCGAGB 1 cut(s) 66
PsuI RGATCY 1 cut(s) 239
PteI GCGCGC 1 cut(s) 522
PvuI CGATCG 1 cut(s) 541
RsaI GTAC 4 cut(s) 101, 222, 434, 775
RsaNI GTAC 4 cut(s) 100, 221, 433, 774
RseI CAYNNNNRTG 1 cut(s) 113
SacI GAGCTC 1 cut(s) 501
SapI GCTCTTC 1 cut(s) 191
SaqAI TTAA 2 cut(s) 258, 567
SatI GCNGC 5 cut(s) 510, 527, 622, 690, 746
Sau3AI GATC 6 cut(s) 117, 170, 178, 239, 514, 538
Sau96I GGNCC 3 cut(s) 310, 461, 556
SchI GAGTC 4 cut(s) 78, 188, 308, 352
ScrFI CCNGG 6 cut(s) 168, 237, 313, 328, 447, 592
SduI GDGCHC 3 cut(s) 159, 419, 501
SetI ASST 9 cut(s) 67, 84, 335, 360, 387, 412, 482, 501, 626
SfaNI GCATC 1 cut(s) 676
Sfr274I CTCGAG 1 cut(s) 66
SinI GGWCC 1 cut(s) 556
SlaI CTCGAG 1 cut(s) 66
SmiMI CAYNNNNRTG 1 cut(s) 113
SmlI CTYRAG 2 cut(s) 66, 572
SmoI CTYRAG 2 cut(s) 66, 572
Sse9I AATT 2 cut(s) 612, 667
SsiI CCGC 6 cut(s) 24, 75, 152, 543, 689, 728
SspMI CTAG 1 cut(s) 811
SstI GAGCTC 1 cut(s) 501
StyD4I CCNGG 6 cut(s) 166, 235, 311, 326, 445, 590
TaaI ACNGT 2 cut(s) 115, 264
TaiI ACGT 4 cut(s) 84, 360, 387, 482
TaqI TCGA 4 cut(s) 67, 120, 192, 627
TasI AATT 2 cut(s) 612, 667
TatI WGTACW 1 cut(s) 773
TauI GCSGC 1 cut(s) 692
TfiI GAWTC 2 cut(s) 547, 751
Tru1I TTAA 2 cut(s) 258, 567
Tru9I TTAA 2 cut(s) 258, 567
TscAI CASTG 2 cut(s) 524, 613
TseI GCWGC 4 cut(s) 509, 526, 621, 745
TspGWI ACGGA 2 cut(s) 297, 591
TspRI CASTG 2 cut(s) 524, 613
VpaK11BI GGWCC 1 cut(s) 556
VspI ATTAAT 1 cut(s) 567
XapI RAATTY 1 cut(s) 612
XhoI CTCGAG 1 cut(s) 66
XspI CTAG 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.