AT1G27320

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014276
Physical Location & Seq
Forward (+)
9486889 .. 9492632
5744 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27320.1

Sequence Viewer

Length: 3111 bp
ATGAGTCTGTTCCATGTGCTAGGGTTTGGTGTCAAGATTGGGCATCTCTTCTGGATGCTATGCTGCTGGTTTGTTTCTTGGTTCGTTGATAATGGGATCGAGGACAAGTCTGGTCTTTTAGTTGGCTCTGTCGGTGATCTTGAGAAGACTAAGATGACTACGTTGAAGAAGAAGAACAAGATGTGGTTCTGGAATAAGATCTCTAGCAGCGGACTCAAGATCCCGAGTTTCTCTTATCAGTTTCTTGGCTCTGTTAAATTCAACAAGGCGTGGTGGAGGAAGCTTGTGGTGGTTTGGGTTGTCTTCTGGGTCTTGGTCTCTATTTGGACGTTTTGGTACTTTAGCTCGCAAGCTATGGAGAAGAGGAAAGAGACGCTAGCTAGTATGTGTGATGAGAGAGCTCGTATGCTGCAGGATCAGTTCAACGTTAGCATGAATCATGTTCAAGCCATGTCTATCTTGATCTCAACCTTCCACCATGGCAAGATTCCTTCTGCTATCGATCAGAGAACATTCTCAGAGTACACTGATAGAACTTCCTTTGAGAGGCCTCTTACTAGCGGGGTAGCTTATGCTATGAGGGTGCTCCATTCAGAGAGGGAAGAGTTCGAGAGGCAACAAGGTTGGACTATTAGGAAGATGTATTCTCTTGAACAAAACCCAGTTCACAAGGATGACTATGACCTGGAAGCTTTGGAACCATCCCCTGTCCAAGAAGAGTACGCTCCAGTCATCTTTGCTCAGGACACTGTTTCTCACGTTGTTTCTCTCGATATGCTGTCTGGGAAAGAAGATCGTGAAAACGTTTTGCGGGCCAGGAGTTCAGGTAAAGGGGTTTTGACAGCTCCTTTCCCATTGATAAAGACAAATAGACTTGGGGTGATCCTGACATTTGCAGTGTACAAGAGAGATCTCCCCTCCAATGCAACGCCAAAAGAGAGAATTGAGGCTACTAACGGGTATCTCGGGGGAGTGTTTGACATTGAGTCCCTGGTAGAAAACTTGCTTCAACAGCTGGCTAGCAAGCAAACGATTCTTGTCAATGTGTACGATATCACCAATCACTCTCAACCGATTAGCATGTATGGTACAAATGTGTCGGCTGATGGGTTGGAACGTGTTAGTCCACTAATCTTTGGCGATCCATTGAGAAAGCATGAGATGCGTTGCAGATTTAAGCAGAAACCACCATGGCCAGTGCTATCAATGGTGACATCATTCGGTATCCTTGTGATTGCGTTACTTGTTGCACATATAATCCACGCAACCGTTAGTCGAATACACAAAGTTGAAGAAGATTGTGATAAAATGAAGCAGCTCAAGAAAAAGGCTGAAGCAGCAGATGTTGCAAAGTCACAGTTCCTTGCCACTGTTTCACATGAAATCAGAACTCCAATGAATGGTGTTCTAGGAATGTTGCATATGCTTATGGACACAGAGTTAGATGTTACGCAACAGGATTATGTTAGGACCGCACAGGCAAGTGGAAAAGCTTTAGTCTCGCTAATAAATGAGGTTTTGGACCAAGCAAAGATTGAATCTGGAAAGCTTGAACTTGAGGAGGTGCGGTTTGATTTGAGAGGAATATTAGATGATGTCCTGTCACTCTTCTCTAGCAAGTCCCAACAAAAGGGGGTGGAGTTGGCAGTATACATATCTGATCGTGTTCCAGATATGTTAATTGGTGATCCTGGGAGGTTTCGACAAATACTCACAAATCTTATGGGTAATTCCATTAAGTTCACTGAGAAAGGACACATCTTTGTAACTGTTCATTTGGTGGATGAGCTATTTGAATCTATCGATGGAGAGACAGCATCATCTCCGGAAAGTACACTGAGTGGGCTTCCAGTTGCAGACCGGCAGAGGAGCTGGGAAAACTTTAAAGCTTTCAGCTCCAACGGGCATCGGAGCTTTGAACCATCTCCCCCTGATATAAACCTAATCGTCTCAGTTGAGGATACTGGCGTAGGGATCCCTGTAGAAGCGCAGTCCCGTATTTTTACGCCTTTCATGCAAGTCGGACCATCCATATCCAGGACGCATGGAGGCACAGGAATTGGACTTAGCATAAGCAAATGTCTAGTTGGACTGATGAAGGGAGAAATTGGATTCTCGAGTACTCCCAAGGTTGGGTCCACATTCACATTTACTGCTGTATTTTCCAATGGGATGCAACCAGCTGAAAGAAAGAATGACAACAACCAGCCCATATTCTCGGAATTCCGGGGCATGAAAGCTGTGGTTGTGGACCATAGGCCTGCAAGGGCAAAAGTCTCGTGGTACCATTTTCAGCGTCTTGGAATTCGAGTCGAAGTAGTTCCACGTGTTGAACAGGCTCTACATTATCTGAAGATTGGTACTACCACTGTGAATATGATACTCATAGAGCAAGAAATATGGAATAGGGAAGCAGATGATTTCATTAAAAAGCTACAGAAAGACCCTCTTTTCCTTTCTCCTAAGTTGATTTTGTTAGCAAACTCAGTAGAATCGTCAATATCAGAGGCTTTATGCACCGGTATAGATCCTCCAATAGTGATAGTGAAACCATTGAGGGCGAGTATGCTAGCAGCAACTTTGCAGAGGGGATTGGGTATTGGAATCAGAGAACCACCTCAACACAAGGGACCTCCTGCTTTGATTCTCAGGAATCTTCTCCTTGGTAGAAAAATTTTAATCGTGGATGATAACAACGTAAACCTCAGAGTGGCAGCGGGAGCTCTGAAAAAGTACGGAGCTGATGTGGTCTGCGCTGAGAGTGGGATAAAGGCAATCTCATTGCTTAAGCCACCTCACGAGTTTGATGCTTGCTTCATGGACATTCAGATGCCAGAAATGGATGGATTTGAAGCTACAAGGAGAATACGAGATATGGAAGAGGAGATGAACAAGAGAATAAAGAATGGGGAGGCTTTGATAGTAGAGAACGGTAACAAAACAAGCTGGCATCTTCCGGTATTAGCAATGACGGCAGATGTGATCCAAGCAACGCATGAGGAATGTCTGAAGTGTGGAATGGATGGGTATGTATCAAAACCATTTGAAGCAGAGCAGCTGTACAGGGAAGTTTCTCGCTTTTTCAATTCGCCTTCAGATACAGAATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009743 GO:0009744 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0009991 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010150 GO:0010271 GO:0010959 GO:0012505 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034285 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042175 GO:0042221 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048366 GO:0048367 GO:0048509 GO:0048519 GO:0048580 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071396 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0090693 GO:0097305 GO:0097306 GO:0098542 GO:0098827 GO:0099402 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1036

Amino Acids

116.37

Weight (kDa)

6.84

Isoelectric Point (pI)

41.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 166 - 362 3.4e-37 CHASE domain
HisKA PF00512 450 - 515 2.2e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 647 - 718 4.2e-16 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 740 - 866 1.5e-36 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 892 - 963 1.3e-13 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27320
fragaria_vesca FvH4_4g23590 FvH4_4g23590 FvH4_4g23590 FvH4_4g23590
malus_domestica MD13G1120900.v1.1 MD16G1068400.v1.1 MD16G1120900.v1.1
prunus_persica Prupe.1G224300_v2.0.a1
pyrus_communis pycom13g10520
rosa_chinensis RchiOBHm_Chr4g0430011
rosa_laevigata RLG00000007014
rosa_roxburghii Rroxscaffold_5G00371820
rosa_rugosa Rorug04G0239800
rosa_samantha Rh4BG302900 Rh4CG318400
rosa_wichuraiana Rw4G025680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 2283
AccB1I GGYRCC 1 cut(s) 2283
AccB7I CCANNNNNTGG 1 cut(s) 1400
AccI GTMKAC 1 cut(s) 1650
AccIII TCCGGA 1 cut(s) 1825
AciI CCGC 6 cut(s) 210, 561, 811, 1473, 1567, 2717
AclI AACGTT 2 cut(s) 426, 804
AcoI YGGCCR 1 cut(s) 1193
AcsI RAATTY 4 cut(s) 257, 2222, 2304, 2673
AcuI CTGAAG 4 cut(s) 1353, 2372, 3029, 3078
AcvI CACGTG 1 cut(s) 2327
AdeI CACNNNGTG 1 cut(s) 1841
AfiI CCNNNNNNNGG 7 cut(s) 546, 1400, 1630, 2037, 2132, 2133, 2710
AflII CTTAAG 1 cut(s) 2786
AflIII ACRYGT 2 cut(s) 1117, 2326
AgeI ACCGGT 1 cut(s) 2519
AhdI GACNNNNNGTC 1 cut(s) 985
AjnI CCWGG 5 cut(s) 684, 815, 990, 1690, 2036
Alw21I GWGCWC 3 cut(s) 403, 588, 2725
Alw26I GTCTC 6 cut(s) 322, 365, 1504, 1805, 1954, 2281
Ama87I CYCGRG 3 cut(s) 223, 965, 2116
Aor13HI TCCGGA 1 cut(s) 1825
AoxI GGCC 4 cut(s) 548, 813, 1193, 2258
ApeKI GCWGC 8 cut(s) 63, 207, 409, 1315, 1337, 2573, 2714, 3055
ApoI RAATTY 4 cut(s) 257, 2222, 2304, 2673
AsiGI ACCGGT 1 cut(s) 2519
Asp700I GAANNNNTTC 1 cut(s) 2319
Asp718I GGTACC 1 cut(s) 2283
AspLEI GCGC 2 cut(s) 1990, 2756
AspS9I GGNCC 7 cut(s) 813, 1470, 1522, 2024, 2136, 2251, 2630
AsuC2I CCSGG 1 cut(s) 2228
AsuHPI GGTGA 5 cut(s) 146, 892, 1048, 1222, 1697
AsuNHI GCTAGC 3 cut(s) 376, 1019, 2569
AvaI CYCGRG 3 cut(s) 223, 965, 2116
AvaII GGWCC 6 cut(s) 1470, 1522, 2024, 2136, 2251, 2630
BaeI ACNNNNGTAYC 4 cut(s) 1080, 1113, 1953, 1986
BalI TGGCCA 1 cut(s) 1195
BamHI GGATCC 1 cut(s) 1974
BanI GGYRCC 1 cut(s) 2283
BanII GRGCYC 2 cut(s) 403, 2725
BauI CACGAG 2 cut(s) 2278, 2798
BbrPI CACGTG 1 cut(s) 2327
BbsI GAAGAC 2 cut(s) 152, 295
Bbv12I GWGCWC 3 cut(s) 403, 588, 2725
BbvI GCAGC 8 cut(s) 50, 219, 396, 1327, 1349, 2585, 2726, 3067
BccI CCATC 7 cut(s) 709, 1100, 1799, 1930, 2035, 2837, 3017
BceAI ACGGC 1 cut(s) 2988
BcgI CGANNNNNNTGC 4 cut(s) 2259, 2293, 2789, 2823
BciT130I CCWGG 5 cut(s) 686, 817, 992, 1692, 2038
BciVI GTATCC 2 cut(s) 1235, 1954
BcnI CCSGG 1 cut(s) 2228
BcoDI GTCTC 6 cut(s) 322, 365, 1504, 1805, 1954, 2281
BfmI CTRYAG 3 cut(s) 410, 1980, 2435
BfrI CTTAAG 1 cut(s) 2786
BfuI GTATCC 2 cut(s) 1235, 1954
BglII AGATCT 2 cut(s) 198, 910
BisI GCNGC 8 cut(s) 64, 208, 410, 1316, 1338, 2574, 2715, 3056
BlsI GCNGC 8 cut(s) 65, 209, 411, 1317, 1339, 2575, 2716, 3057
BmcAI AGTACT 1 cut(s) 2122
Bme1390I CCNGG 6 cut(s) 686, 817, 992, 1692, 2038, 2228
Bme18I GGWCC 6 cut(s) 1470, 1522, 2024, 2136, 2251, 2630
BmeRI GACNNNNNGTC 1 cut(s) 985
BmeT110I CYCGRG 3 cut(s) 223, 965, 2116
BmgT120I GGNCC 7 cut(s) 813, 1470, 1522, 2024, 2136, 2251, 2630
BmiI GGNNCC 5 cut(s) 699, 1976, 2137, 2285, 2631
BmrFI CCNGG 6 cut(s) 686, 817, 992, 1692, 2038, 2228
BmrI ACTGGG 1 cut(s) 656
BmsI GCATC 9 cut(s) 45, 52, 1152, 1826, 1915, 2163, 2797, 2820, 2959
BmtI GCTAGC 3 cut(s) 380, 1023, 2573
BmuI ACTGGG 1 cut(s) 656
BpiI GAAGAC 2 cut(s) 152, 295
BpmI CTGGAG 1 cut(s) 711
Bpu10I CCTNAGC 1 cut(s) 741
BpuEI CTTGAG 4 cut(s) 161, 200, 1304, 1577
BpuMI CCSGG 1 cut(s) 2228
Bsa29I ATCGAT 2 cut(s) 501, 1803
BsaAI YACGTR 1 cut(s) 2327
BsaI GGTCTC 1 cut(s) 322
BsaJI CCNNGG 7 cut(s) 478, 990, 1190, 1691, 2127, 2227, 2662
BsaWI WCCGGW 3 cut(s) 1825, 2519, 2956
BsaXI ACNNNNNCTCC 6 cut(s) 811, 841, 1553, 1583, 2730, 2760
Bsc4I CCNNNNNNNGG 7 cut(s) 546, 1400, 1630, 2037, 2132, 2133, 2710
Bse118I RCCGGY 2 cut(s) 1860, 2519
Bse1I ACTGG 5 cut(s) 662, 728, 1196, 1850, 1969
Bse3DI GCAATG 2 cut(s) 2780, 2973
BseAI TCCGGA 1 cut(s) 1825
BseBI CCWGG 5 cut(s) 686, 817, 992, 1692, 2038
BseCI ATCGAT 2 cut(s) 501, 1803
BseDI CCNNGG 7 cut(s) 478, 990, 1190, 1691, 2127, 2227, 2662
BseGI GGATG 9 cut(s) 60, 679, 701, 1789, 2027, 2178, 2692, 2848, 3028
BseLI CCNNNNNNNGG 7 cut(s) 546, 1400, 1630, 2037, 2132, 2133, 2710
BseMI GCAATG 2 cut(s) 2780, 2973
BseMII CTCAG 9 cut(s) 531, 755, 1737, 1829, 1965, 2499, 2662, 2719, 2748
BseNI ACTGG 5 cut(s) 662, 728, 1196, 1850, 1969
BseRI GAGGAG 3 cut(s) 1574, 1882, 2897
BseXI GCAGC 8 cut(s) 50, 219, 396, 1327, 1349, 2585, 2726, 3067
BseYI CCCAGC 1 cut(s) 1872
BshFI GGCC 4 cut(s) 550, 815, 1195, 2260
BshNI GGYRCC 1 cut(s) 2283
BshTI ACCGGT 1 cut(s) 2519
BshVI ATCGAT 2 cut(s) 501, 1803
BsiHKAI GWGCWC 3 cut(s) 403, 588, 2725
BsiHKCI CYCGRG 3 cut(s) 223, 965, 2116
BsiSI CCGG 5 cut(s) 1826, 1861, 2227, 2520, 2957
BslFI GGGAC 4 cut(s) 973, 1606, 1978, 2643
BslI CCNNNNNNNGG 7 cut(s) 546, 1400, 1630, 2037, 2132, 2133, 2710
BsmAI GTCTC 6 cut(s) 322, 365, 1504, 1805, 1954, 2281
BsmBI CGTCTC 2 cut(s) 365, 1954
BsmFI GGGAC 4 cut(s) 973, 1606, 1978, 2643
BsnI GGCC 4 cut(s) 550, 815, 1195, 2260
Bso31I GGTCTC 1 cut(s) 322
BsoBI CYCGRG 3 cut(s) 223, 965, 2116
Bsp1286I GDGCHC 3 cut(s) 403, 588, 2725
Bsp13I TCCGGA 1 cut(s) 1825
Bsp1407I TGTACA 2 cut(s) 900, 3060
Bsp19I CCATGG 2 cut(s) 478, 1190
BspACI CCGC 6 cut(s) 210, 561, 811, 1473, 1567, 2717
BspANI GGCC 4 cut(s) 550, 815, 1195, 2260
BspCNI CTCAG 9 cut(s) 530, 754, 1738, 1830, 1964, 2498, 2661, 2718, 2749
BspDI ATCGAT 2 cut(s) 501, 1803
BspEI TCCGGA 1 cut(s) 1825
BspLI GGNNCC 5 cut(s) 699, 1976, 2137, 2285, 2631
BspMAI CTGCAG 1 cut(s) 414
BspOI GCTAGC 3 cut(s) 380, 1023, 2573
BspT107I GGYRCC 1 cut(s) 2283
BspTI CTTAAG 1 cut(s) 2786
BspTNI GGTCTC 1 cut(s) 322
BsrDI GCAATG 2 cut(s) 2780, 2973
BsrFI RCCGGY 2 cut(s) 1860, 2519
BsrGI TGTACA 2 cut(s) 900, 3060
BsrI ACTGG 5 cut(s) 662, 728, 1196, 1850, 1969
BssAI RCCGGY 2 cut(s) 1860, 2519
BssECI CCNNGG 7 cut(s) 478, 990, 1190, 1691, 2127, 2227, 2662
BssNAI GTATAC 1 cut(s) 1651
BssSI CACGAG 2 cut(s) 2278, 2798
BssT1I CCWWGG 4 cut(s) 478, 1190, 2127, 2662
Bst1107I GTATAC 1 cut(s) 1651
Bst2BI CACGAG 2 cut(s) 2278, 2798
Bst2UI CCWGG 5 cut(s) 686, 817, 992, 1692, 2038
Bst4CI ACNGT 7 cut(s) 751, 1270, 1359, 1372, 1771, 2371, 2933
Bst6I CTCTTC 6 cut(s) 53, 356, 597, 711, 1613, 2874
BstAFI CTTAAG 1 cut(s) 2786
BstAPI GCANNNNNTGC 2 cut(s) 1162, 1346
BstAUI TGTACA 2 cut(s) 900, 3060
BstBAI YACGTR 1 cut(s) 2327
BstDSI CCRYGG 2 cut(s) 478, 1190
BstENI CCTNNNNNAGG 1 cut(s) 544
BstF5I GGATG 9 cut(s) 60, 679, 701, 1789, 2027, 2178, 2692, 2848, 3028
BstHHI GCGC 2 cut(s) 1990, 2756
BstMAI GTCTC 6 cut(s) 322, 365, 1504, 1805, 1954, 2281
BstMWI GCNNNNNNNGC 7 cut(s) 1012, 1162, 1337, 1346, 2014, 2720, 2972
BstNI CCWGG 5 cut(s) 686, 817, 992, 1692, 2038
BstNSI RCATGY 1 cut(s) 1084
BstSCI CCNGG 6 cut(s) 684, 815, 990, 1690, 2036, 2226
BstSFI CTRYAG 3 cut(s) 410, 1980, 2435
BstV1I GCAGC 8 cut(s) 50, 219, 396, 1327, 1349, 2585, 2726, 3067
BstV2I GAAGAC 2 cut(s) 152, 295
BstX2I RGATCY 5 cut(s) 198, 219, 910, 1974, 2527
BstYI RGATCY 5 cut(s) 198, 219, 910, 1974, 2527
BstZ17I GTATAC 1 cut(s) 1651
Bsu15I ATCGAT 2 cut(s) 501, 1803
BsuI GTATCC 2 cut(s) 1235, 1954
BsuRI GGCC 4 cut(s) 550, 815, 1195, 2260
BsuTUI ATCGAT 2 cut(s) 501, 1803
BtgI CCRYGG 2 cut(s) 478, 1190
BtsCI GGATG 9 cut(s) 60, 679, 701, 1789, 2027, 2178, 2692, 2848, 3028
BtsI GCAGTG 1 cut(s) 903
BtsIMutI CAGTG 8 cut(s) 525, 747, 903, 1203, 1368, 1743, 1835, 2367
CfoI GCGC 2 cut(s) 1990, 2756
Cfr10I RCCGGY 2 cut(s) 1860, 2519
Cfr13I GGNCC 7 cut(s) 813, 1470, 1522, 2024, 2136, 2251, 2630
ClaI ATCGAT 2 cut(s) 501, 1803
CseI GACGC 3 cut(s) 382, 2050, 2285
CspAI ACCGGT 1 cut(s) 2519
DraI TTTAAA 1 cut(s) 1885
DraIII CACNNNGTG 1 cut(s) 1841
DriI GACNNNNNGTC 1 cut(s) 985
EaeI YGGCCR 1 cut(s) 1193
Eam1104I CTCTTC 6 cut(s) 53, 356, 597, 711, 1613, 2874
Eam1105I GACNNNNNGTC 1 cut(s) 985
EarI CTCTTC 6 cut(s) 53, 356, 597, 711, 1613, 2874
Ecl136II GAGCTC 2 cut(s) 401, 2723
Eco130I CCWWGG 4 cut(s) 478, 1190, 2127, 2662
Eco147I AGGCCT 2 cut(s) 550, 2260
Eco24I GRGCYC 2 cut(s) 403, 2725
Eco31I GGTCTC 1 cut(s) 322
Eco32I GATATC 1 cut(s) 1054
Eco47I GGWCC 6 cut(s) 1470, 1522, 2024, 2136, 2251, 2630
Eco53kI GAGCTC 2 cut(s) 401, 2723
Eco57I CTGAAG 4 cut(s) 1353, 2372, 3029, 3078
Eco72I CACGTG 1 cut(s) 2327
Eco88I CYCGRG 3 cut(s) 223, 965, 2116
EcoICRI GAGCTC 2 cut(s) 401, 2723
EcoNI CCTNNNNNAGG 1 cut(s) 544
EcoO109I RGGNCCY 1 cut(s) 2630
EcoRI GAATTC 2 cut(s) 2222, 2304
EcoRII CCWGG 5 cut(s) 684, 815, 990, 1690, 2036
EcoRV GATATC 1 cut(s) 1054
EcoT14I CCWWGG 4 cut(s) 478, 1190, 2127, 2662
EcoT38I GRGCYC 2 cut(s) 403, 2725
ErhI CCWWGG 4 cut(s) 478, 1190, 2127, 2662
Esp3I CGTCTC 2 cut(s) 365, 1954
FalI AAGNNNNNCTT 2 cut(s) 2433, 2465
FaqI GGGAC 4 cut(s) 973, 1606, 1978, 2643
FauI CCCGC 3 cut(s) 554, 804, 2710
FauNDI CATATG 1 cut(s) 1422
FblI GTMKAC 1 cut(s) 1650
Fnu4HI GCNGC 8 cut(s) 64, 208, 410, 1316, 1338, 2574, 2715, 3056
FokI GGATG 9 cut(s) 67, 686, 688, 1796, 2014, 2185, 2699, 2855, 3035
FriOI GRGCYC 2 cut(s) 403, 2725
Fsp4HI GCNGC 8 cut(s) 64, 208, 410, 1316, 1338, 2574, 2715, 3056
GlaI GCGC 2 cut(s) 1989, 2755
GluI GCNGC 8 cut(s) 64, 208, 410, 1316, 1338, 2574, 2715, 3056
GsaI CCCAGC 1 cut(s) 1876
GsuI CTGGAG 1 cut(s) 711
HaeIII GGCC 4 cut(s) 550, 815, 1195, 2260
HapII CCGG 5 cut(s) 1826, 1861, 2227, 2520, 2957
HgaI GACGC 3 cut(s) 382, 2050, 2285
HhaI GCGC 2 cut(s) 1990, 2756
Hin6I GCGC 2 cut(s) 1988, 2754
HinP1I GCGC 2 cut(s) 1988, 2754
HindIII AAGCTT 5 cut(s) 281, 690, 1491, 1547, 1887
HpaII CCGG 5 cut(s) 1826, 1861, 2227, 2520, 2957
HphI GGTGA 5 cut(s) 146, 892, 1048, 1222, 1697
HpyAV CCTTC 4 cut(s) 481, 501, 2092, 3102
HpyCH4III ACNGT 7 cut(s) 751, 1270, 1359, 1372, 1771, 2371, 2933
HpyCH4IV ACGT 8 cut(s) 161, 329, 426, 759, 804, 1117, 2326, 2697
HpyF10VI GCNNNNNNNGC 7 cut(s) 1012, 1162, 1337, 1346, 2014, 2720, 2972
HpySE526I ACGT 8 cut(s) 161, 329, 426, 759, 804, 1117, 2326, 2697
HspAI GCGC 2 cut(s) 1988, 2754
Kpn2I TCCGGA 1 cut(s) 1825
KpnI GGTACC 1 cut(s) 2287
LmnI GCTCC 8 cut(s) 591, 730, 850, 1869, 1901, 1911, 2720, 2738
Lsp1109I GCAGC 8 cut(s) 50, 219, 396, 1327, 1349, 2585, 2726, 3067
LweI GCATC 9 cut(s) 45, 52, 1152, 1826, 1915, 2163, 2797, 2820, 2959
MaeII ACGT 8 cut(s) 161, 329, 426, 759, 804, 1117, 2326, 2697
MaeIII GTNAC 7 cut(s) 1210, 1239, 1353, 1447, 1602, 1765, 2933
MflI RGATCY 5 cut(s) 198, 219, 910, 1974, 2527
MhlI GDGCHC 3 cut(s) 403, 588, 2725
MlsI TGGCCA 1 cut(s) 1195
MluNI TGGCCA 1 cut(s) 1195
MlyI GAGTC 4 cut(s) 13, 207, 995, 2319
MmeI TCCRAC 5 cut(s) 605, 1092, 1923, 2002, 2068
Mox20I TGGCCA 1 cut(s) 1195
MroI TCCGGA 1 cut(s) 1825
MroXI GAANNNNTTC 1 cut(s) 2319
MscI TGGCCA 1 cut(s) 1195
MseI TTAA 8 cut(s) 255, 1176, 1679, 1737, 1884, 2427, 2678, 2787
MslI CAYNNNNRTG 2 cut(s) 672, 2828
Msp20I TGGCCA 1 cut(s) 1195
MspA1I CMGCKG 5 cut(s) 210, 1015, 2183, 2717, 3058
MspCI CTTAAG 1 cut(s) 2786
MspI CCGG 5 cut(s) 1826, 1861, 2227, 2520, 2957
MspR9I CCNGG 6 cut(s) 686, 817, 992, 1692, 2038, 2228
MvaI CCWGG 5 cut(s) 686, 817, 992, 1692, 2038
MwoI GCNNNNNNNGC 7 cut(s) 1012, 1162, 1337, 1346, 2014, 2720, 2972
NciI CCSGG 1 cut(s) 2228
NcoI CCATGG 2 cut(s) 478, 1190
NdeI CATATG 1 cut(s) 1422
NheI GCTAGC 3 cut(s) 376, 1019, 2569
NlaIV GGNNCC 5 cut(s) 699, 1976, 2137, 2285, 2631
NmuCI GTSAC 3 cut(s) 1210, 1353, 1602
NspI RCATGY 1 cut(s) 1084
PaeR7I CTCGAG 1 cut(s) 2116
PceI AGGCCT 2 cut(s) 550, 2260
PdmI GAANNNNTTC 1 cut(s) 2319
PflMI CCANNNNNTGG 1 cut(s) 1400
PfoI TCCNGGA 1 cut(s) 2036
PinAI ACCGGT 1 cut(s) 2519
PkrI GCNGC 8 cut(s) 65, 209, 411, 1317, 1339, 2575, 2716, 3057
PleI GAGTC 4 cut(s) 12, 207, 994, 2318
PmaCI CACGTG 1 cut(s) 2327
PmlI CACGTG 1 cut(s) 2327
PpsI GAGTC 4 cut(s) 12, 207, 994, 2318
Ppu21I YACGTR 1 cut(s) 2327
PpuMI RGGWCCY 1 cut(s) 2630
Psp124BI GAGCTC 2 cut(s) 403, 2725
Psp1406I AACGTT 2 cut(s) 426, 804
Psp5II RGGWCCY 1 cut(s) 2630
Psp6I CCWGG 5 cut(s) 684, 815, 990, 1690, 2036
PspCI CACGTG 1 cut(s) 2327
PspFI CCCAGC 1 cut(s) 1872
PspGI CCWGG 5 cut(s) 684, 815, 990, 1690, 2036
PspN4I GGNNCC 5 cut(s) 699, 1976, 2137, 2285, 2631
PspPI GGNCC 7 cut(s) 813, 1470, 1522, 2024, 2136, 2251, 2630
PspPPI RGGWCCY 1 cut(s) 2630
PsrI GAACNNNNNNTAC 2 cut(s) 2325, 2357
PstI CTGCAG 1 cut(s) 414
PsuI RGATCY 5 cut(s) 198, 219, 910, 1974, 2527
PvuII CAGCTG 3 cut(s) 1015, 2183, 3058
RseI CAYNNNNRTG 2 cut(s) 672, 2828
SacI GAGCTC 2 cut(s) 403, 2725
SaqAI TTAA 8 cut(s) 255, 1176, 1679, 1737, 1884, 2427, 2678, 2787
SatI GCNGC 8 cut(s) 64, 208, 410, 1316, 1338, 2574, 2715, 3056
Sau96I GGNCC 7 cut(s) 813, 1470, 1522, 2024, 2136, 2251, 2630
ScaI AGTACT 1 cut(s) 2122
SchI GAGTC 4 cut(s) 13, 207, 995, 2319
ScrFI CCNGG 6 cut(s) 686, 817, 992, 1692, 2038, 2228
SduI GDGCHC 3 cut(s) 403, 588, 2725
SfaNI GCATC 9 cut(s) 45, 52, 1152, 1826, 1915, 2163, 2797, 2820, 2959
SfcI CTRYAG 3 cut(s) 410, 1980, 2435
Sfr274I CTCGAG 1 cut(s) 2116
SinI GGWCC 6 cut(s) 1470, 1522, 2024, 2136, 2251, 2630
SlaI CTCGAG 1 cut(s) 2116
SmiMI CAYNNNNRTG 2 cut(s) 672, 2828
SmlI CTYRAG 6 cut(s) 140, 215, 1319, 1556, 2116, 2786
SmoI CTYRAG 6 cut(s) 140, 215, 1319, 1556, 2116, 2786
SseBI AGGCCT 2 cut(s) 550, 2260
SsiI CCGC 6 cut(s) 210, 561, 811, 1473, 1567, 2717
SspI AATATT 1 cut(s) 1587
SstI GAGCTC 2 cut(s) 403, 2725
StuI AGGCCT 2 cut(s) 550, 2260
StyD4I CCNGG 6 cut(s) 684, 815, 990, 1690, 2036, 2226
StyI CCWWGG 4 cut(s) 478, 1190, 2127, 2662
TaaI ACNGT 7 cut(s) 751, 1270, 1359, 1372, 1771, 2371, 2933
TaiI ACGT 8 cut(s) 164, 332, 429, 762, 807, 1120, 2329, 2700
TatI WGTACW 5 cut(s) 522, 900, 1832, 2120, 3060
Tru1I TTAA 8 cut(s) 255, 1176, 1679, 1737, 1884, 2427, 2678, 2787
Tru9I TTAA 8 cut(s) 255, 1176, 1679, 1737, 1884, 2427, 2678, 2787
TscAI CASTG 8 cut(s) 532, 754, 903, 1203, 1375, 1750, 1842, 2374
TseFI GTSAC 3 cut(s) 1210, 1353, 1602
TseI GCWGC 8 cut(s) 63, 207, 409, 1315, 1337, 2573, 2714, 3055
Tsp45I GTSAC 3 cut(s) 1210, 1353, 1602
TspGWI ACGGA 1 cut(s) 2751
TspRI CASTG 8 cut(s) 532, 754, 903, 1203, 1375, 1750, 1842, 2374
Van91I CCANNNNNTGG 1 cut(s) 1400
Vha464I CTTAAG 1 cut(s) 2786
VpaK11BI GGWCC 6 cut(s) 1470, 1522, 2024, 2136, 2251, 2630
XagI CCTNNNNNAGG 1 cut(s) 544
XapI RAATTY 4 cut(s) 257, 2222, 2304, 2673
XceI RCATGY 1 cut(s) 1084
XhoI CTCGAG 1 cut(s) 2116
XmiI GTMKAC 1 cut(s) 1650
XmnI GAANNNNTTC 1 cut(s) 2319
ZrmI AGTACT 1 cut(s) 2122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.