pycom13g10520

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014276
Physical Location & Seq
Forward (+)
7049238 .. 7054095
4858 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g10520.2

Sequence Viewer

Length: 3099 bp
ATGAGTGTTTTCCATGTATTTGGGTTCGGTTTAAAGGTGGGGCATATGCTTTGGATGCTATGTTGCTGGATTGTATCTGTAATTTCCATGAACTGGTACCTCACTGGTGGGATTGTGACAGACACCAAGGCAGGATTGCTTGGTGAAGTTGCCAATATGTGCCTCAAATGGTGGGAGAAAGTCCCGATGAACATCAGCAAGATCCGCTACCATTACTACCAGTATATCGGGTCGAAGAGAGTCCGAAAAACGTGGTGGAAGGGGCTTTTGTTTACGTGGGTGGTTGGATGGACAATTGGGAGCCTCTGGATCTTATGGTACATGAGCTCACAGGCTAGTGAGAAGAGGAAGGAGACTCTTGCAAGTATGTGTGATGAGAGGGCTAGGATGTTGCAGGATCAGTTTAATGTCAGCATGAACCATATTCAGGCCATGTCGATACTGATCTCGACATTCCACCACGGGAAGTACCCGTCTGCCATTGATCAGAAAACTTTTGCGCGGTACACTGAAAGAACTGCTTTTGAGAGACCCCTCACAAGTGGCGTGGCATATGCTGTAAGGGTGCTCCACTCGGAAAAGGAACAATTTGAGAAGCAGCAGGGCTGGACTATAAAGAGAATGGATACCCTCGAACAAAACCAGGTTCATAAGAACGACTATGCCCCAGAAGCCTTGGAACCATCCCCAGTTCAGGAAGAATATGCTCCTGTTATCTTTGCACAGGATACCGTTAGACACATCATCTCTTATGATATGCTGTCCGGGAAGGAAGATCGACAAAACGTGTTGCGTGCAAGAGAATCAGGAAAGGGGGTTCTAACTGCTCCTTTTCGGCTGCTTAAAACAAATCGCCTTGGAGTAATACTGACATTTGCTGTCTACAAAAGAGATCTGCCCTCAAATGCAACTCCAAATGAAAGAATTCAAGCGACTGATGGGTATCTCGGTGGAATCTTTCATATTGAGTCACTTGTGGAGAAGTTACTTCAACAACTTGCTAGCAAGCAAACCATCCTTGTGAACGTGTATGACACTACCAATCAGTTGCATCCAATCAGCATGTACGGTTCAAATGTTTCAGATGATGGATTGCAGCACATAAGCTCCTTAAGTTTTGGAGATCCCTTAAGGAACCATGAGATGCGGTGCAGGTTCAAACATAAGCCACCATGGCCGTGGTTAGCCATTACAACTTCTTTTGGGATCCTTGTGATTGCATTGCTTGTTGGATACATTTTCCATGCAACTGTAAATAGGATTGCCAAAGTGGAGGATGATTTCCGTAAGATGATGGAGCTCAAGAAACAGGCTGAGGCAGCTGATGTTGCCAAATCTCAGTTTCTTGCAACTGTTTCACACGAGATCAGAACCCCAATGAATGGTGTCCTAGGAATGTTACATATGCTAATGGACACAAATCTGGACGTTACTCAACTAGATTATGTCCGAACTGCCCAGGGAAGTGGAAAAGCTCTTGTCTCACTTATAAATGAGGTGTTGGACCAGGCAAAGATTGAATCTGGTAAGCTGGAGCTTGAGGCGGTGCGTTTTGATCTGCGAGCTATTCTGGATGATGTTTTGTCACTCTTTTCAGGGAAGTCTCAAGAAAAAGGAGTAGAGGTTCCCGATATGCTTATTGGTGATCCCGGAAGGTTTCGGCAGATTATCACCAATCTCATTGGGAATTCAATCAAATTCACAGAGAAAGGACACATCTTTGTCACGGTTCATCTTGTCGAGGAGCTGATTGGCTCGATAGATGTTGAGACTGAATCATCATCAAAGAACACCTTGAGCGGTTTCCCTGTAGCAGATAGACACTGCAGTTGGGGAGGATTTAGGTGTTTTGGTCAAGATGGATCGACGAATCAATTCTCATCCTCCTCTGACCTCATCAATATTATTGTATCTGTTGAGGACACAGGAGTAGGAATCCCTCTAGAAGCACAATCTCGTGTTTTCACTCCTTTTATGCAGGTGGGACCATCCATCTCACGTACACATGGAGGCACAGGAATTGGGCTAAGCATAAGCAAGTGTTTGGTTGGCCTCATGAAAGGGGAAATCGGGTTTGTGAGCATTCCCAAGATTGGTTCCACATTTACATTTACTGTTGTTTTCACCAATGCCAGCTCCAATTCAAACGAGTTAACAATCCAGCAAATGAACAGCCAGTCAAATGTTGCATCCTCAGAATTTAATGGCATGACGGCATTAGTAGTGGACCAAAGACCTGTTAGGGCCAAGATGTCAAGATATCATATCCAACGGCTTGGAATTTGTGTTGAAGTAGTTTCTGATTTGAATCAGGGTTTATCTAGCATAAGCTCTGGGATTACCACCATCAATATGGTCCTTGTTGAACAGGAAGTCTGGGACAAAGATTCGGGCACCTCAGCTCTCTTTGTCAATAATTTACGGAAAATTGATGGACAGGTTCCTCCGAAACTGTTCATTCTCACCAATTCTATAAGCTCATGCAGAATAAGTTCTGCAATTTCAGGTGTCTCTACCCCAACTGTCATCATGAAGCCTCTCAGGGCAAGCATGCTTGCTGCCTCACTACAACGAGCCATGGGAGTTGGTAACAAGGGAAATCTCCGGAACGGGGACCTCCCAAGTTTGTCTCTCAGCAATCTTCTGCTCGGGAGAATAATTCTTATTATAGATGATAATAATGTGAATCTCAGAGTAGCTGCTGGTGCTTTGAAGAAGTATGGGGCTGAGGTGGTCTGTGCAGACAGTGGTAAAAAGGCAATCTCACTGCTTACGCCGCCCCACCATTTTGATGCCTGCTTCATGGATATCCAGATGCCAGAGATGGACGGGTTTGAAGCTACAAGGAGAATACGCAACATGGAACGCAATATCAGTAACCGTATTCAGCATGGCGAAGTTTCTGCAGGGGATTCTGAAAATATTCAAGCATGGCATGTACCCATTCTAGCCATGACTGCAGACGTAATTCAGGCAACCCATGAGGAATGTACAAGGTGCGGAATGGATGGATATGTTTCAAAACCATTCGAAGCTGAACAGCTTTATCGCGAAGTTTCACGTTTTTTCCAATCTACAACAACTGGGAGCTTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009743 GO:0009744 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0009991 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010150 GO:0010271 GO:0010959 GO:0012505 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034285 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042175 GO:0042221 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048366 GO:0048367 GO:0048509 GO:0048519 GO:0048580 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071396 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0090693 GO:0097305 GO:0097306 GO:0098542 GO:0098827 GO:0099402 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1033

Amino Acids

114.91

Weight (kDa)

7.62

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27320
fragaria_vesca FvH4_4g23590 FvH4_4g23590 FvH4_4g23590 FvH4_4g23590
malus_domestica MD13G1120900.v1.1 MD16G1068400.v1.1 MD16G1120900.v1.1
prunus_persica Prupe.1G224300_v2.0.a1
pyrus_communis pycom13g10520
rosa_chinensis RchiOBHm_Chr4g0430011
rosa_laevigata RLG00000007014
rosa_roxburghii Rroxscaffold_5G00371820
rosa_rugosa Rorug04G0239800
rosa_samantha Rh4BG302900 Rh4CG318400
rosa_wichuraiana Rw4G025680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1490
AarI CACCTGC 1 cut(s) 1969
Acc36I ACCTGC 2 cut(s) 1143, 1969
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 2 cut(s) 96, 2393
AccB7I CCANNNNNTGG 2 cut(s) 93, 1382
AccBSI CCGCTC 1 cut(s) 1800
AccI GTMKAC 1 cut(s) 882
AccII CGCG 2 cut(s) 502, 3051
AccIII TCCGGA 1 cut(s) 2604
AciI CCGC 7 cut(s) 205, 502, 1147, 1544, 1800, 2777, 3000
AclWI GGATC 8 cut(s) 196, 317, 405, 1118, 1201, 1214, 1640, 1870
AcoI YGGCCR 1 cut(s) 1175
AcsI RAATTY 5 cut(s) 924, 1687, 1697, 2198, 2280
AfaI GTAC 8 cut(s) 98, 320, 470, 506, 1067, 2002, 2940, 2992
AfiI CCNNNNNNNGG 6 cut(s) 93, 427, 694, 1382, 2093, 2611
AflII CTTAAG 2 cut(s) 1111, 1129
AflIII ACRYGT 2 cut(s) 786, 1026
AjnI CCWGG 3 cut(s) 642, 1458, 1506
AloI GAACNNNNNNTCC 4 cut(s) 801, 833, 1608, 1640
Alw21I GWGCWC 3 cut(s) 329, 570, 1302
Alw26I GTCTC 7 cut(s) 347, 523, 1486, 1608, 1763, 2515, 2634
AlwI GGATC 8 cut(s) 196, 317, 405, 1118, 1201, 1214, 1640, 1870
Ama87I CYCGRG 1 cut(s) 2648
Aor13HI TCCGGA 1 cut(s) 2604
AoxI GGCC 4 cut(s) 429, 1175, 2050, 2244
ApeKI GCWGC 6 cut(s) 598, 838, 1096, 1319, 2558, 2699
ApoI RAATTY 5 cut(s) 924, 1687, 1697, 2198, 2280
Asp700I GAANNNNTTC 5 cut(s) 924, 1874, 2453, 2491, 2922
Asp718I GGTACC 1 cut(s) 96
AspA2I CCTAGG 1 cut(s) 1390
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 6 cut(s) 1504, 1985, 2227, 2244, 2356, 2614
AsuC2I CCSGG 2 cut(s) 766, 1650
AsuHPI GGTGA 5 cut(s) 155, 1655, 1663, 2116, 2455
AsuII TTCGAA 1 cut(s) 3030
AsuNHI GCTAGC 1 cut(s) 1001
AvaI CYCGRG 1 cut(s) 2648
AvaII GGWCC 5 cut(s) 1504, 1985, 2227, 2356, 2614
AvrII CCTAGG 1 cut(s) 1390
BaeGI GKGCMC 1 cut(s) 2396
BamHI GGATCC 1 cut(s) 1206
BanI GGYRCC 2 cut(s) 96, 2393
BanII GRGCYC 2 cut(s) 329, 1302
BauI CACGAG 2 cut(s) 1361, 1956
Bbv12I GWGCWC 3 cut(s) 329, 570, 1302
BbvCI CCTCAGC 3 cut(s) 1314, 2398, 2727
BbvI GCAGC 6 cut(s) 610, 825, 1108, 1331, 2545, 2686
BceAI ACGGC 3 cut(s) 1162, 2229, 2288
BcgI CGANNNNNNTGC 2 cut(s) 2885, 2919
BciT130I CCWGG 3 cut(s) 644, 1460, 1508
BciVI GTATCC 3 cut(s) 619, 721, 1226
BclI TGATCA 1 cut(s) 484
BcnI CCSGG 2 cut(s) 766, 1650
BcoDI GTCTC 7 cut(s) 347, 523, 1486, 1608, 1763, 2515, 2634
BfaI CTAG 8 cut(s) 336, 384, 1002, 1391, 1439, 1943, 2322, 2948
BfmI CTRYAG 4 cut(s) 1809, 1825, 2904, 2958
BfrI CTTAAG 2 cut(s) 1111, 1129
BfuAI ACCTGC 2 cut(s) 1143, 1969
BfuI GTATCC 3 cut(s) 619, 721, 1226
BglI GCCNNNNNGGC 1 cut(s) 1174
BglII AGATCT 1 cut(s) 892
BisI GCNGC 7 cut(s) 599, 839, 1097, 1320, 2559, 2700, 2777
BlnI CCTAGG 1 cut(s) 1390
BlpI GCTNAGC 1 cut(s) 2027
BlsI GCNGC 7 cut(s) 600, 840, 1098, 1321, 2560, 2701, 2778
Bme1390I CCNGG 5 cut(s) 644, 766, 1460, 1508, 1650
Bme18I GGWCC 5 cut(s) 1504, 1985, 2227, 2356, 2614
BmeT110I CYCGRG 1 cut(s) 2648
BmgT120I GGNCC 6 cut(s) 1504, 1985, 2227, 2244, 2356, 2614
BmrFI CCNGG 5 cut(s) 644, 766, 1460, 1508, 1650
BmrI ACTGGG 2 cut(s) 683, 3093
BmsI GCATC 6 cut(s) 45, 1060, 1134, 2198, 2782, 2805
BmtI GCTAGC 1 cut(s) 1005
BmuI ACTGGG 2 cut(s) 683, 3093
BpmI CTGGAG 1 cut(s) 1553
Bpu10I CCTNAGC 3 cut(s) 1314, 2398, 2727
Bpu1102I GCTNAGC 1 cut(s) 2027
Bpu14I TTCGAA 1 cut(s) 3030
BpuEI CTTGAG 4 cut(s) 1286, 1559, 1590, 1816
BpuMI CCSGG 2 cut(s) 766, 1650
BsaAI YACGTR 2 cut(s) 276, 2000
BsaBI GATNNNNATC 4 cut(s) 191, 443, 942, 2307
BsaI GGTCTC 1 cut(s) 523
BsaWI WCCGGW 1 cut(s) 2604
BsaXI ACNNNNNCTCC 4 cut(s) 1091, 1121, 1608, 1638
Bsc4I CCNNNNNNNGG 6 cut(s) 93, 427, 694, 1382, 2093, 2611
Bse1I ACTGG 6 cut(s) 98, 109, 220, 689, 2176, 3088
Bse3DI GCAATG 1 cut(s) 1220
Bse8I GATNNNNATC 4 cut(s) 191, 443, 942, 2307
BseAI TCCGGA 1 cut(s) 2604
BseBI CCWGG 3 cut(s) 644, 1460, 1508
BseJI GATNNNNATC 4 cut(s) 191, 443, 942, 2307
BseLI CCNNNNNNNGG 6 cut(s) 93, 427, 694, 1382, 2093, 2611
BseMI GCAATG 1 cut(s) 1220
BseMII CTCAG 8 cut(s) 1305, 1352, 2208, 2412, 2554, 2647, 2704, 2718
BseNI ACTGG 6 cut(s) 98, 109, 220, 689, 2176, 3088
BseRI GAGGAG 2 cut(s) 1757, 1876
BseSI GKGCMC 1 cut(s) 2396
BseXI GCAGC 6 cut(s) 610, 825, 1108, 1331, 2545, 2686
BsgI GTGCAG 2 cut(s) 1171, 2760
Bsh1236I CGCG 2 cut(s) 502, 3051
BshFI GGCC 4 cut(s) 431, 1177, 2052, 2246
BshNI GGYRCC 2 cut(s) 96, 2393
BsiHKAI GWGCWC 3 cut(s) 329, 570, 1302
BsiHKCI CYCGRG 1 cut(s) 2648
BsiSI CCGG 3 cut(s) 765, 1650, 2605
BslFI GGGAC 4 cut(s) 167, 1998, 2393, 2627
BslI CCNNNNNNNGG 6 cut(s) 93, 427, 694, 1382, 2093, 2611
BsmAI GTCTC 7 cut(s) 347, 523, 1486, 1608, 1763, 2515, 2634
BsmFI GGGAC 4 cut(s) 167, 1998, 2393, 2627
BsmI GAATGC 1 cut(s) 2082
BsnI GGCC 4 cut(s) 431, 1177, 2052, 2246
Bso31I GGTCTC 1 cut(s) 523
BsoBI CYCGRG 1 cut(s) 2648
Bsp119I TTCGAA 1 cut(s) 3030
Bsp1286I GDGCHC 4 cut(s) 329, 570, 1302, 2396
Bsp13I TCCGGA 1 cut(s) 2604
Bsp1407I TGTACA 1 cut(s) 2990
Bsp1720I GCTNAGC 1 cut(s) 2027
Bsp19I CCATGG 2 cut(s) 1172, 2577
Bsp68I TCGCGA 1 cut(s) 3051
BspACI CCGC 7 cut(s) 205, 502, 1147, 1544, 1800, 2777, 3000
BspANI GGCC 4 cut(s) 431, 1177, 2052, 2246
BspCNI CTCAG 8 cut(s) 1306, 1351, 2207, 2411, 2553, 2646, 2703, 2719
BspEI TCCGGA 1 cut(s) 2604
BspFNI CGCG 2 cut(s) 502, 3051
BspHI TCATGA 2 cut(s) 2055, 2529
BspMAI CTGCAG 3 cut(s) 1829, 2908, 2962
BspMI ACCTGC 2 cut(s) 1143, 1969
BspOI GCTAGC 1 cut(s) 1005
BspPI GGATC 8 cut(s) 196, 317, 405, 1118, 1201, 1214, 1640, 1870
BspT104I TTCGAA 1 cut(s) 3030
BspT107I GGYRCC 2 cut(s) 96, 2393
BspTI CTTAAG 2 cut(s) 1111, 1129
BspTNI GGTCTC 1 cut(s) 523
BsrBI CCGCTC 1 cut(s) 1800
BsrDI GCAATG 1 cut(s) 1220
BsrGI TGTACA 1 cut(s) 2990
BsrI ACTGG 6 cut(s) 98, 109, 220, 689, 2176, 3088
BssSI CACGAG 2 cut(s) 1361, 1956
BssT1I CCWWGG 6 cut(s) 126, 675, 856, 1172, 1390, 2577
Bst2BI CACGAG 2 cut(s) 1361, 1956
Bst2UI CCWGG 3 cut(s) 644, 1460, 1508
Bst6I CTCTTC 2 cut(s) 230, 338
BstAFI CTTAAG 2 cut(s) 1111, 1129
BstAUI TGTACA 1 cut(s) 2990
BstBAI YACGTR 2 cut(s) 276, 2000
BstBI TTCGAA 1 cut(s) 3030
BstC8I GCNNGC 9 cut(s) 795, 1003, 1007, 1563, 2134, 2548, 2552, 2556, 2797
BstDEI CTNAG 9 cut(s) 1314, 1338, 2027, 2194, 2398, 2540, 2633, 2690, 2727
BstDSI CCRYGG 4 cut(s) 460, 1172, 1178, 2577
BstFNI CGCG 2 cut(s) 502, 3051
BstHHI GCGC 1 cut(s) 502
BstMAI GTCTC 7 cut(s) 347, 523, 1486, 1608, 1763, 2515, 2634
BstMWI GCNNNNNNNGC 9 cut(s) 55, 204, 671, 1174, 1319, 1328, 2705, 2776, 2957
BstNI CCWGG 3 cut(s) 644, 1460, 1508
BstNSI RCATGY 3 cut(s) 1066, 2554, 2939
BstSCI CCNGG 5 cut(s) 642, 764, 1458, 1506, 1648
BstSFI CTRYAG 4 cut(s) 1809, 1825, 2904, 2958
BstSLI GKGCMC 1 cut(s) 2396
BstUI CGCG 2 cut(s) 502, 3051
BstV1I GCAGC 6 cut(s) 610, 825, 1108, 1331, 2545, 2686
BstX2I RGATCY 5 cut(s) 201, 309, 892, 1123, 1206
BstXI CCANNNNNNTGG 5 cut(s) 20, 1179, 1466, 2276, 2353
BstYI RGATCY 5 cut(s) 201, 309, 892, 1123, 1206
BsuI GTATCC 3 cut(s) 619, 721, 1226
BsuRI GGCC 4 cut(s) 431, 1177, 2052, 2246
BtgI CCRYGG 4 cut(s) 460, 1172, 1178, 2577
BtsI GCAGTG 2 cut(s) 1822, 2765
BtsIMutI CAGTG 5 cut(s) 102, 507, 1822, 2752, 2765
BtuMI TCGCGA 1 cut(s) 3051
BveI ACCTGC 2 cut(s) 1143, 1969
Cac8I GCNNGC 9 cut(s) 795, 1003, 1007, 1563, 2134, 2548, 2552, 2556, 2797
CciI TCATGA 2 cut(s) 2055, 2529
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 6 cut(s) 1504, 1985, 2227, 2244, 2356, 2614
CsiI ACCWGGT 1 cut(s) 642
Csp6I GTAC 8 cut(s) 97, 319, 469, 505, 1066, 2001, 2939, 2991
CspCI CAANNNNNGTGG 2 cut(s) 528, 563
CviQI GTAC 8 cut(s) 97, 319, 469, 505, 1066, 2001, 2939, 2991
DdeI CTNAG 9 cut(s) 1314, 1338, 2027, 2194, 2398, 2540, 2633, 2690, 2727
DraI TTTAAA 1 cut(s) 33
EaeI YGGCCR 1 cut(s) 1175
Eam1104I CTCTTC 2 cut(s) 230, 338
EarI CTCTTC 2 cut(s) 230, 338
Ecl136II GAGCTC 2 cut(s) 327, 1300
Eco130I CCWWGG 6 cut(s) 126, 675, 856, 1172, 1390, 2577
Eco24I GRGCYC 2 cut(s) 329, 1302
Eco31I GGTCTC 1 cut(s) 523
Eco32I GATATC 2 cut(s) 2261, 2809
Eco47I GGWCC 5 cut(s) 1504, 1985, 2227, 2356, 2614
Eco53kI GAGCTC 2 cut(s) 327, 1300
Eco88I CYCGRG 1 cut(s) 2648
EcoICRI GAGCTC 2 cut(s) 327, 1300
EcoO109I RGGNCCY 1 cut(s) 2614
EcoRI GAATTC 2 cut(s) 924, 1687
EcoRII CCWGG 3 cut(s) 642, 1458, 1506
EcoRV GATATC 2 cut(s) 2261, 2809
EcoT14I CCWWGG 6 cut(s) 126, 675, 856, 1172, 1390, 2577
EcoT38I GRGCYC 2 cut(s) 329, 1302
ErhI CCWWGG 6 cut(s) 126, 675, 856, 1172, 1390, 2577
FalI AAGNNNNNCTT 4 cut(s) 505, 537, 1778, 1810
FaqI GGGAC 4 cut(s) 167, 1998, 2393, 2627
FauNDI CATATG 3 cut(s) 45, 553, 1404
FbaI TGATCA 1 cut(s) 484
FblI GTMKAC 1 cut(s) 882
Fnu4HI GCNGC 7 cut(s) 599, 839, 1097, 1320, 2559, 2700, 2777
FriOI GRGCYC 2 cut(s) 329, 1302
Fsp4HI GCNGC 7 cut(s) 599, 839, 1097, 1320, 2559, 2700, 2777
FspBI CTAG 8 cut(s) 336, 384, 1002, 1391, 1439, 1943, 2322, 2948
GlaI GCGC 1 cut(s) 501
GluI GCNGC 7 cut(s) 599, 839, 1097, 1320, 2559, 2700, 2777
GsuI CTGGAG 1 cut(s) 1553
HaeIII GGCC 4 cut(s) 431, 1177, 2052, 2246
HapII CCGG 3 cut(s) 765, 1650, 2605
HhaI GCGC 1 cut(s) 502
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 1 cut(s) 2154
HindII GTYRAC 1 cut(s) 2154
HpaI GTTAAC 1 cut(s) 2154
HpaII CCGG 3 cut(s) 765, 1650, 2605
HphI GGTGA 5 cut(s) 155, 1655, 1663, 2116, 2455
Hpy166II GTNNAC 7 cut(s) 273, 507, 883, 1024, 2003, 2154, 2227
Hpy8I GTNNAC 7 cut(s) 273, 507, 883, 1024, 2003, 2154, 2227
Hpy99I CGWCG 1 cut(s) 1870
HpyAV CCTTC 4 cut(s) 253, 343, 763, 1647
HpyCH4IV ACGT 8 cut(s) 251, 275, 786, 1026, 1428, 1999, 2964, 3061
HpyF10VI GCNNNNNNNGC 9 cut(s) 55, 204, 671, 1174, 1319, 1328, 2705, 2776, 2957
HpyF3I CTNAG 9 cut(s) 1314, 1338, 2027, 2194, 2398, 2540, 2633, 2690, 2727
HpySE526I ACGT 8 cut(s) 251, 275, 786, 1026, 1428, 1999, 2964, 3061
HspAI GCGC 1 cut(s) 500
Kpn2I TCCGGA 1 cut(s) 2604
KpnI GGTACC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 484
KspAI GTTAAC 1 cut(s) 2154
Lsp1109I GCAGC 6 cut(s) 610, 825, 1108, 1331, 2545, 2686
LweI GCATC 6 cut(s) 45, 1060, 1134, 2198, 2782, 2805
MabI ACCWGGT 1 cut(s) 642
MaeI CTAG 8 cut(s) 336, 384, 1002, 1391, 1439, 1943, 2322, 2948
MaeII ACGT 8 cut(s) 251, 275, 786, 1026, 1428, 1999, 2964, 3061
MaeIII GTNAC 9 cut(s) 115, 969, 984, 1398, 1429, 1584, 1723, 2588, 2876
MbiI CCGCTC 1 cut(s) 1800
MboII GAAGA 6 cut(s) 247, 355, 710, 785, 2633, 2725
MfeI CAATTG 1 cut(s) 294
MflI RGATCY 5 cut(s) 201, 309, 892, 1123, 1206
MhlI GDGCHC 4 cut(s) 329, 570, 1302, 2396
MlyI GAGTC 3 cut(s) 249, 349, 977
MmeI TCCRAC 4 cut(s) 265, 1210, 1482, 2293
MroI TCCGGA 1 cut(s) 2604
MroXI GAANNNNTTC 5 cut(s) 924, 1874, 2453, 2491, 2922
MseI TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2153, 2202
MslI CAYNNNNRTG 4 cut(s) 1019, 1177, 2351, 2790
MspA1I CMGCKG 1 cut(s) 1322
MspCI CTTAAG 2 cut(s) 1111, 1129
MspI CCGG 3 cut(s) 765, 1650, 2605
MspR9I CCNGG 5 cut(s) 644, 766, 1460, 1508, 1650
MunI CAATTG 1 cut(s) 294
Mva1269I GAATGC 1 cut(s) 2082
MvaI CCWGG 3 cut(s) 644, 1460, 1508
MvnI CGCG 2 cut(s) 502, 3051
MwoI GCNNNNNNNGC 9 cut(s) 55, 204, 671, 1174, 1319, 1328, 2705, 2776, 2957
NciI CCSGG 2 cut(s) 766, 1650
NcoI CCATGG 2 cut(s) 1172, 2577
NdeI CATATG 3 cut(s) 45, 553, 1404
NheI GCTAGC 1 cut(s) 1001
NmuCI GTSAC 4 cut(s) 115, 969, 1584, 1723
NruI TCGCGA 1 cut(s) 3051
NspI RCATGY 3 cut(s) 1066, 2554, 2939
NspV TTCGAA 1 cut(s) 3030
PaeI GCATGC 1 cut(s) 2554
PagI TCATGA 2 cut(s) 2055, 2529
PaqCI CACCTGC 1 cut(s) 1969
PasI CCCWGGG 1 cut(s) 1459
PctI GAATGC 1 cut(s) 2082
PdmI GAANNNNTTC 5 cut(s) 924, 1874, 2453, 2491, 2922
PflMI CCANNNNNTGG 2 cut(s) 93, 1382
PfoI TCCNGGA 2 cut(s) 764, 1648
PkrI GCNGC 7 cut(s) 600, 840, 1098, 1321, 2560, 2701, 2778
PleI GAGTC 3 cut(s) 248, 349, 976
PpsI GAGTC 3 cut(s) 248, 349, 976
Ppu21I YACGTR 2 cut(s) 276, 2000
PpuMI RGGWCCY 1 cut(s) 2614
PsiI TTATAA 1 cut(s) 1490
Psp124BI GAGCTC 2 cut(s) 329, 1302
Psp5II RGGWCCY 1 cut(s) 2614
Psp6I CCWGG 3 cut(s) 642, 1458, 1506
PspGI CCWGG 3 cut(s) 642, 1458, 1506
PspPI GGNCC 6 cut(s) 1504, 1985, 2227, 2244, 2356, 2614
PspPPI RGGWCCY 1 cut(s) 2614
PstI CTGCAG 3 cut(s) 1829, 2908, 2962
PsuI RGATCY 5 cut(s) 201, 309, 892, 1123, 1206
PvuII CAGCTG 1 cut(s) 1322
RruI TCGCGA 1 cut(s) 3051
RsaI GTAC 8 cut(s) 98, 320, 470, 506, 1067, 2002, 2940, 2992
RsaNI GTAC 8 cut(s) 97, 319, 469, 505, 1066, 2001, 2939, 2991
RseI CAYNNNNRTG 4 cut(s) 1019, 1177, 2351, 2790
SacI GAGCTC 2 cut(s) 329, 1302
SaqAI TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2153, 2202
SatI GCNGC 7 cut(s) 599, 839, 1097, 1320, 2559, 2700, 2777
Sau96I GGNCC 6 cut(s) 1504, 1985, 2227, 2244, 2356, 2614
SchI GAGTC 3 cut(s) 249, 349, 977
ScrFI CCNGG 5 cut(s) 644, 766, 1460, 1508, 1650
SduI GDGCHC 4 cut(s) 329, 570, 1302, 2396
SexAI ACCWGGT 1 cut(s) 642
SfaNI GCATC 6 cut(s) 45, 1060, 1134, 2198, 2782, 2805
SfcI CTRYAG 4 cut(s) 1809, 1825, 2904, 2958
SfuI TTCGAA 1 cut(s) 3030
SinI GGWCC 5 cut(s) 1504, 1985, 2227, 2356, 2614
SmiMI CAYNNNNRTG 4 cut(s) 1019, 1177, 2351, 2790
SmlI CTYRAG 6 cut(s) 1111, 1129, 1301, 1538, 1605, 1795
SmoI CTYRAG 6 cut(s) 1111, 1129, 1301, 1538, 1605, 1795
SphI GCATGC 1 cut(s) 2554
SsiI CCGC 7 cut(s) 205, 502, 1147, 1544, 1800, 2777, 3000
SspI AATATT 2 cut(s) 1903, 2923
SspMI CTAG 8 cut(s) 336, 384, 1002, 1391, 1439, 1943, 2322, 2948
SstI GAGCTC 2 cut(s) 329, 1302
StyD4I CCNGG 5 cut(s) 642, 764, 1458, 1506, 1648
StyI CCWWGG 6 cut(s) 126, 675, 856, 1172, 1390, 2577
TaiI ACGT 8 cut(s) 254, 278, 789, 1029, 1431, 2002, 2967, 3064
TaqI TCGA 9 cut(s) 233, 437, 449, 633, 778, 1740, 1757, 1865, 3030
TatI WGTACW 1 cut(s) 2990
TauI GCSGC 1 cut(s) 2779
Tru1I TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2153, 2202
Tru9I TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2153, 2202
TscAI CASTG 5 cut(s) 109, 514, 1829, 2752, 2772
TseFI GTSAC 4 cut(s) 115, 969, 1584, 1723
TseI GCWGC 6 cut(s) 598, 838, 1096, 1319, 2558, 2699
Tsp45I GTSAC 4 cut(s) 115, 969, 1584, 1723
TspGWI ACGGA 2 cut(s) 1274, 2437
TspRI CASTG 5 cut(s) 109, 514, 1829, 2752, 2772
Van91I CCANNNNNTGG 2 cut(s) 93, 1382
Vha464I CTTAAG 2 cut(s) 1111, 1129
VpaK11BI GGWCC 5 cut(s) 1504, 1985, 2227, 2356, 2614
XapI RAATTY 5 cut(s) 924, 1687, 1697, 2198, 2280
XbaI TCTAGA 1 cut(s) 1942
XceI RCATGY 3 cut(s) 1066, 2554, 2939
XcmI CCANNNNNNNNNTGG 2 cut(s) 1176, 2350
XmaJI CCTAGG 1 cut(s) 1390
XmiI GTMKAC 1 cut(s) 882
XmnI GAANNNNTTC 5 cut(s) 924, 1874, 2453, 2491, 2922
XspI CTAG 8 cut(s) 336, 384, 1002, 1391, 1439, 1943, 2322, 2948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.