RLG00000007014

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014276
Physical Location & Seq
Forward (+)
12683660 .. 12688637
4978 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007014

Sequence Viewer

Length: 3033 bp
ATGAATTGGTACCTTAATGGTGGAATTATGGACACCAAGAGTGGTGGTTTGCTTGGTGATGGCGCCAATTTGTGCCTCAAATTGTGGGAGAAGATCCCATTGAACATTACTAAGATCCGCCATCATTACTACCAGTATATTGGGTCCAAGAGAGTTCGGAAGACTTGGTGGAAGAGGCTCTTGGTTTCCTGGGTGGTTGGTTGGACTATTGCTTGCTTTTGGATCTTCTGGTACATGAGCTCGGTGGCTAGTGAGAAGAGGAAGGAGACTCTTACGAGCATGTGTGATGAGAGAGCAAGGATGTTGCAGGATCAGTTTAATGTGAGCATGAACCATATTCAGGCCATGTCGATTCTGATCTCGACATTCCACCACGGCAAGTATCCATCTGCAATTGACCAGAGAACTTTTGCGAGGTACACTGAAAGAACTGCTTTTGAGAGACCTCTTACAAGTGGCGTGGCATATGCTGTAAGGGTGCTCCACTCGGAGAAAGAACAATTTGAGAAGCAGCAAGGCTGGACTATCAAGAGAATGGATACCCTCGAACACAACCAAGTCCATAAGAATGACTATACCCCAGAACTGCTGGAGCCACCCCCAATTCAGGAAGAATATGCTCCTGTCATATTTGCTCAGGATACCGTTGCACATGTCATTTCTTTCGATATGCTGTCTGGGAAGGAAGATCGAGATAATGTGTTGCGCGCAAGAGAATCAGGAAAGGGGGTCTTGACTGCTCCATTTCGGCTGCTTAAAACTAATAGGCTTGGAGTAATACTGACCTTCGCTGTCTACAAGAGAGATCTACCTTCAAATGCAACTCCAAATGAAAGAATTCAAGCAACTGATGGGTATCTAGGCGGAATCTTTCATATTGAGTCACTTGTGGAGAAGTTACTTCAACAACTTGCAAGCAAGCAAACTATCCTTGTCAATGTGTACGATACTACCAATCATTCGGAGCCAATCAGCATGTATGGTTCAAATGTTTCAGATGATGGATTGCGGCATATTAGTACCTTAAATTTTGGAGATCCCTTAAGGAAGCATGAGATGCACTGCAGATTCAAACATAAGCCACCGTGGCCGTGGTTAGCCATCACAACTTCAATTGGGATCCTTGTGATTGTGTTGCTTGTTGGGCATATTTTTCATGCAACTGTAAATAGGATTGCCAAAGTGGAAGATGATTTCCATAAAATGAGCGATCTCAAGAAACAGGCCGAAGCAGCTGATGTTGCCAAATCTCAGTTCCTTGCAACTGTATCCCATGAGATTAGAACCCCAATGAACGGTGTCCTAGGTATGTTGCACATGCTTATGGATACAGATCTAGATGTAACTCAACAAGATTATGTCAGAACTGCCCAGGGTAGTGGAAAAGCCCTAGTCTCACTTATAAATGAGGTCTTGGACCAGGCAAAAATTGAATCTGGTAAGCTGGAGCTCGAGGCAGTGCGTTTTGATCTGCGAGCAATTTTGGATGATGTTTTGTCACTGTTTTCTGGGAAGTCTCAAGAAAAAGGAGTGGAGCTGGGAGTATACATCTCAGATCAGGTTCCTGAAATGCTTATTGGTGATCCTGGAAGGTTTCGGCAAATTATTACCAATCTCATGGGGAATTCGATCAAATTCACAGAGAAAGGACACATCTTTGTCACTGTCCATTTGGTTGAGGAGCTGATTGACTCGATAGACGTTGAGACTGAATCATCATTGAAAAACACTTTGAGTGGGTTCCCTGTTGCGGATAAACGCCGCAGCTGGGGAGGATTTAGGGGTTTCAGTGAAGAGGGATCTGCAAGCTCGTTCTCATCATCGTCTGATGTCATCAATCTTATTGTGTCTGTTGAGGACACAGGGGTTGGAATCCCTCTAGAAGCCCAATCTCGTGTTTTCACTCCTTTTATGCAGGTGGGACCATCTATCTCACGGACACATGGTGGCACAGGAATTGGGCTAAGCATAAGCAAGTGTTTAGTTGGCCTCATGAGAGGGGAGATTGGTTTTGTGAGCATTCCCAAGATTGGTTCCACATTTACATTTACCGCTGTTTTCGCCAAAGCCCGCTGTGATTCGAATGAATTTAAGATCCAGCAAATCAACAGCCAAGCAAATGCTGCATCCTCAGACTTTCATGGCATGACAGCATTGGTTGTGGATCACAGACCTGTGAGGGCCAAGATGTCAAGATATCATATCCAAAGGCTTGGAATTCATGTTGAAGTCGCTTTTGAGTTACATCAGGCTTTGTCTTGCATAAGCTCTGGGAATACGACTATAAATATGGTCCTTGTTGAACAGGAGGTTTGGGATAATGATTCTGGCAGTTCAGCTCTCTTTATTGATAACTTAAAGAAACTTAAATGTCCGGTTCCTCCTAAGCTGTTCCTTCTTGCCAATTCTATAAGCAGTTGCAGAACAAGCTTTGTGAATTCAGGTGTCTGTACGCCAAGTATTATCATGAAGCCTCTCAGGGCAAGTATGCTTGCTGCCTCACTACAAAGAGCCATGGGAATTGGTAACAAGGGTAATACACGGAATGGTGAGCTCCCAAGTTTGTCTCTCCGCAATCTTCTACTTGGAAGAAAAATTCTTATTGTGGATGATAATAAAGTGAACCTCATAGTAGCTGCTGGTGCTTTGAAAAAGTATGGGGCTACAGTGAAGAGTGCGGACAGCGGTAAAGAGGCAATCTCACTGCTTACACCACCCCATAGTTTTGACGCCTGCTTCATGGATATCCAGATGCCGGAAATGGATGGTTTTGAAGCTACAAGGAGAATACGCGACATAGAACGCAATGTCAGCAACAATATTCAACATGGCGAGGTTTCTGCGGAGGACTATGGCAATATTTTATCATGGCATGTACCCATTCTGGCCATGACTGCAGATGTTATTCAGGCCACCCATGAGGAATGCACAAGGTGTGGAATGGATGGATACGTTTCTAAGCCATTTGAAGCTGAACAGCTTTATCGAGAAGTTTCACGTTTTCTCCAATCTCCTGCAAAAGGTACTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009743 GO:0009744 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0009991 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010150 GO:0010271 GO:0010959 GO:0012505 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034285 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042175 GO:0042221 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048366 GO:0048367 GO:0048509 GO:0048519 GO:0048580 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071396 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0090693 GO:0097305 GO:0097306 GO:0098542 GO:0098827 GO:0099402 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1011

Amino Acids

112.58

Weight (kDa)

7.41

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 131 - 327 3.7e-38 CHASE domain
HisKA PF00512 415 - 480 4.2e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 613 - 684 4e-14 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 710 - 840 1.3e-43 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 866 - 936 5.8e-15 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27320
fragaria_vesca FvH4_4g23590 FvH4_4g23590 FvH4_4g23590 FvH4_4g23590
malus_domestica MD13G1120900.v1.1 MD16G1068400.v1.1 MD16G1120900.v1.1
prunus_persica Prupe.1G224300_v2.0.a1
pyrus_communis pycom13g10520
rosa_chinensis RchiOBHm_Chr4g0430011
rosa_laevigata RLG00000007014
rosa_roxburghii Rroxscaffold_5G00371820
rosa_rugosa Rorug04G0239800
rosa_samantha Rh4BG302900 Rh4CG318400
rosa_wichuraiana Rw4G025680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1403
AarI CACCTGC 1 cut(s) 1906
Acc36I ACCTGC 1 cut(s) 1906
Acc65I GGTACC 1 cut(s) 9
AccB1I GGYRCC 2 cut(s) 9, 62
AccI GTMKAC 2 cut(s) 795, 1545
AccII CGCG 2 cut(s) 708, 2793
AcoI YGGCCR 2 cut(s) 1088, 2886
AcsI RAATTY 8 cut(s) 837, 1027, 1624, 1634, 2087, 2217, 2437, 2595
AcyI GRCGYC 2 cut(s) 63, 2730
AdeI CACNNNGTG 2 cut(s) 1946, 2934
AfaI GTAC 8 cut(s) 11, 233, 419, 944, 1021, 2452, 2877, 3025
AfiI CCNNNNNNNGG 8 cut(s) 340, 607, 1295, 1750, 1768, 2030, 2755, 3020
AflII CTTAAG 1 cut(s) 1042
AflIII ACRYGT 1 cut(s) 652
AjnI CCWGG 4 cut(s) 188, 1371, 1419, 1585
AjuI GAANNNNNNNTTGG 6 cut(s) 164, 196, 1238, 1270, 1560, 1592
Alw21I GWGCWC 4 cut(s) 242, 483, 1452, 2556
Alw26I GTCTC 6 cut(s) 260, 436, 1399, 1521, 1700, 2571
Ama87I CYCGRG 1 cut(s) 1451
AoxI GGCC 7 cut(s) 342, 1088, 1224, 1987, 2181, 2886, 2910
ApeKI GCWGC 7 cut(s) 511, 751, 1232, 1764, 2123, 2495, 2636
ApoI RAATTY 8 cut(s) 837, 1027, 1624, 1634, 2087, 2217, 2437, 2595
Asp700I GAANNNNTTC 1 cut(s) 837
Asp718I GGTACC 1 cut(s) 9
AspA2I CCTAGG 1 cut(s) 1303
AspLEI GCGC 3 cut(s) 65, 708, 710
AspS9I GGNCC 5 cut(s) 144, 1417, 1922, 2181, 2293
AsuHPI GGTGA 3 cut(s) 68, 1592, 2561
AsuII TTCGAA 1 cut(s) 2081
AvaI CYCGRG 1 cut(s) 1451
AvaII GGWCC 4 cut(s) 144, 1417, 1922, 2293
AvrII CCTAGG 1 cut(s) 1303
BalI TGGCCA 1 cut(s) 2888
BamHI GGATCC 1 cut(s) 1119
BanI GGYRCC 2 cut(s) 9, 62
BanII GRGCYC 3 cut(s) 242, 1452, 2556
BauI CACGAG 1 cut(s) 1893
BbsI GAAGAC 1 cut(s) 167
Bbv12I GWGCWC 4 cut(s) 242, 483, 1452, 2556
BbvI GCAGC 7 cut(s) 523, 738, 1244, 1776, 2110, 2482, 2623
BccI CCATC 9 cut(s) 53, 129, 394, 845, 995, 1109, 1933, 2759, 2939
BceAI ACGGC 2 cut(s) 391, 1075
BcgI CGANNNNNNTGC 2 cut(s) 2822, 2856
BciT130I CCWGG 4 cut(s) 190, 1373, 1421, 1587
BciVI GTATCC 6 cut(s) 393, 532, 634, 1279, 1321, 2942
BcoDI GTCTC 6 cut(s) 260, 436, 1399, 1521, 1700, 2571
BfaI CTAG 6 cut(s) 249, 860, 1304, 1337, 1391, 1880
BfmI CTRYAG 3 cut(s) 1063, 2664, 2895
BfoI RGCGCY 1 cut(s) 66
BfrI CTTAAG 1 cut(s) 1042
BfuAI ACCTGC 1 cut(s) 1906
BfuI GTATCC 6 cut(s) 393, 532, 634, 1279, 1321, 2942
BglI GCCNNNNNGGC 1 cut(s) 1087
BglII AGATCT 2 cut(s) 805, 1333
BisI GCNGC 9 cut(s) 512, 752, 1010, 1233, 1762, 1765, 2124, 2496, 2637
BlnI CCTAGG 1 cut(s) 1303
BlpI GCTNAGC 1 cut(s) 1964
BlsI GCNGC 9 cut(s) 513, 753, 1011, 1234, 1763, 1766, 2125, 2497, 2638
Bme1390I CCNGG 4 cut(s) 190, 1373, 1421, 1587
Bme18I GGWCC 4 cut(s) 144, 1417, 1922, 2293
BmeT110I CYCGRG 1 cut(s) 1451
BmgT120I GGNCC 5 cut(s) 144, 1417, 1922, 2181, 2293
BmrFI CCNGG 4 cut(s) 190, 1373, 1421, 1587
BmsI GCATC 3 cut(s) 1047, 2135, 2742
BpiI GAAGAC 1 cut(s) 167
BplI GAGNNNNNCTC 2 cut(s) 2684, 2716
BpmI CTGGAG 2 cut(s) 611, 1466
Bpu10I CCTNAGC 2 cut(s) 636, 2385
Bpu1102I GCTNAGC 1 cut(s) 1964
Bpu14I TTCGAA 1 cut(s) 2081
BpuEI CTTGAG 2 cut(s) 1199, 1503
BsaBI GATNNNNATC 3 cut(s) 356, 855, 1332
BsaHI GRCGYC 2 cut(s) 63, 2730
BsaI GGTCTC 1 cut(s) 436
BsaJI CCNNGG 8 cut(s) 189, 373, 1085, 1091, 1303, 1371, 1372, 2514
BsaWI WCCGGW 1 cut(s) 2374
BsaXI ACNNNNNCTCC 2 cut(s) 2988, 3018
Bsc4I CCNNNNNNNGG 8 cut(s) 340, 607, 1295, 1750, 1768, 2030, 2755, 3020
Bse1I ACTGG 1 cut(s) 133
Bse3DI GCAATG 1 cut(s) 2812
Bse8I GATNNNNATC 3 cut(s) 356, 855, 1332
BseBI CCWGG 4 cut(s) 190, 1373, 1421, 1587
BseDI CCNNGG 8 cut(s) 189, 373, 1085, 1091, 1303, 1371, 1372, 2514
BseGI GGATG 6 cut(s) 306, 1492, 2126, 2614, 2770, 2950
BseJI GATNNNNATC 3 cut(s) 356, 855, 1332
BseLI CCNNNNNNNGG 8 cut(s) 340, 607, 1295, 1750, 1768, 2030, 2755, 3020
BseMI GCAATG 1 cut(s) 2812
BseMII CTCAG 5 cut(s) 650, 1265, 1566, 2145, 2491
BseNI ACTGG 1 cut(s) 133
BsePI GCGCGC 1 cut(s) 706
BseRI GAGGAG 1 cut(s) 1694
BseXI GCAGC 7 cut(s) 523, 738, 1244, 1776, 2110, 2482, 2623
BseYI CCCAGC 2 cut(s) 1537, 1767
Bsh1236I CGCG 2 cut(s) 708, 2793
BshFI GGCC 7 cut(s) 344, 1090, 1226, 1989, 2183, 2888, 2912
BshNI GGYRCC 2 cut(s) 9, 62
BsiHKAI GWGCWC 4 cut(s) 242, 483, 1452, 2556
BsiHKCI CYCGRG 1 cut(s) 1451
BsiSI CCGG 2 cut(s) 2375, 2756
BslFI GGGAC 1 cut(s) 1935
BslI CCNNNNNNNGG 8 cut(s) 340, 607, 1295, 1750, 1768, 2030, 2755, 3020
BsmAI GTCTC 6 cut(s) 260, 436, 1399, 1521, 1700, 2571
BsmFI GGGAC 1 cut(s) 1935
BsmI GAATGC 2 cut(s) 2019, 2930
BsnI GGCC 7 cut(s) 344, 1090, 1226, 1989, 2183, 2888, 2912
Bso31I GGTCTC 1 cut(s) 436
BsoBI CYCGRG 1 cut(s) 1451
Bsp119I TTCGAA 1 cut(s) 2081
Bsp1286I GDGCHC 4 cut(s) 242, 483, 1452, 2556
Bsp1720I GCTNAGC 1 cut(s) 1964
Bsp19I CCATGG 1 cut(s) 2514
BspANI GGCC 7 cut(s) 344, 1090, 1226, 1989, 2183, 2888, 2912
BspCNI CTCAG 5 cut(s) 649, 1264, 1565, 2144, 2490
BspFNI CGCG 2 cut(s) 708, 2793
BspHI TCATGA 2 cut(s) 1992, 2466
BspMAI CTGCAG 2 cut(s) 1067, 2899
BspMI ACCTGC 1 cut(s) 1906
BspT104I TTCGAA 1 cut(s) 2081
BspT107I GGYRCC 2 cut(s) 9, 62
BspTI CTTAAG 1 cut(s) 1042
BspTNI GGTCTC 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 2812
BsrI ACTGG 1 cut(s) 133
BssECI CCNNGG 8 cut(s) 189, 373, 1085, 1091, 1303, 1371, 1372, 2514
BssHII GCGCGC 1 cut(s) 706
BssNAI GTATAC 1 cut(s) 1546
BssNI GRCGYC 2 cut(s) 63, 2730
BssSI CACGAG 1 cut(s) 1893
BssT1I CCWWGG 2 cut(s) 1303, 2514
Bst1107I GTATAC 1 cut(s) 1546
Bst2BI CACGAG 1 cut(s) 1893
Bst2UI CCWGG 4 cut(s) 190, 1373, 1421, 1587
Bst4CI ACNGT 8 cut(s) 646, 1086, 1165, 1267, 1298, 1503, 1666, 2668
Bst6I CTCTTC 4 cut(s) 167, 251, 1788, 2666
BstACI GRCGYC 2 cut(s) 63, 2730
BstAFI CTTAAG 1 cut(s) 1042
BstAPI GCANNNNNTGC 2 cut(s) 1057, 2123
BstBI TTCGAA 1 cut(s) 2081
BstC8I GCNNGC 9 cut(s) 214, 708, 916, 920, 1476, 1807, 2071, 2493, 2734
BstDEI CTNAG 9 cut(s) 111, 636, 1251, 1552, 1964, 2131, 2385, 2477, 2958
BstDSI CCRYGG 4 cut(s) 373, 1085, 1091, 2514
BstENI CCTNNNNNAGG 1 cut(s) 3018
BstF5I GGATG 6 cut(s) 306, 1492, 2126, 2614, 2770, 2950
BstFNI CGCG 2 cut(s) 708, 2793
BstH2I RGCGCY 1 cut(s) 66
BstHHI GCGC 3 cut(s) 65, 708, 710
BstMAI GTCTC 6 cut(s) 260, 436, 1399, 1521, 1700, 2571
BstNI CCWGG 4 cut(s) 190, 1373, 1421, 1587
BstNSI RCATGY 5 cut(s) 283, 656, 979, 1321, 2876
BstSCI CCNGG 4 cut(s) 188, 1371, 1419, 1585
BstSFI CTRYAG 3 cut(s) 1063, 2664, 2895
BstUI CGCG 2 cut(s) 708, 2793
BstV1I GCAGC 7 cut(s) 523, 738, 1244, 1776, 2110, 2482, 2623
BstV2I GAAGAC 1 cut(s) 167
BstX2I RGATCY 9 cut(s) 93, 114, 222, 805, 1036, 1119, 1333, 1799, 2094
BstXI CCANNNNNNTGG 4 cut(s) 140, 1379, 1618, 2213
BstYI RGATCY 9 cut(s) 93, 114, 222, 805, 1036, 1119, 1333, 1799, 2094
BstZ17I GTATAC 1 cut(s) 1546
BsuI GTATCC 6 cut(s) 393, 532, 634, 1279, 1321, 2942
BsuRI GGCC 7 cut(s) 344, 1090, 1226, 1989, 2183, 2888, 2912
BtgI CCRYGG 4 cut(s) 373, 1085, 1091, 2514
BtsCI GGATG 6 cut(s) 306, 1492, 2126, 2614, 2770, 2950
BtsI GCAGTG 3 cut(s) 1060, 1464, 2702
BtsIMutI CAGTG 8 cut(s) 420, 1060, 1464, 1499, 1662, 1795, 2673, 2702
BveI ACCTGC 1 cut(s) 1906
Cac8I GCNNGC 9 cut(s) 214, 708, 916, 920, 1476, 1807, 2071, 2493, 2734
CciI TCATGA 2 cut(s) 1992, 2466
CfoI GCGC 3 cut(s) 65, 708, 710
Cfr13I GGNCC 5 cut(s) 144, 1417, 1922, 2181, 2293
CseI GACGC 1 cut(s) 2738
Csp6I GTAC 8 cut(s) 10, 232, 418, 943, 1020, 2451, 2876, 3024
CspCI CAANNNNNGTGG 4 cut(s) 25, 60, 441, 476
CviQI GTAC 8 cut(s) 10, 232, 418, 943, 1020, 2451, 2876, 3024
DdeI CTNAG 9 cut(s) 111, 636, 1251, 1552, 1964, 2131, 2385, 2477, 2958
DinI GGCGCC 1 cut(s) 64
DraIII CACNNNGTG 2 cut(s) 1946, 2934
EaeI YGGCCR 2 cut(s) 1088, 2886
Eam1104I CTCTTC 4 cut(s) 167, 251, 1788, 2666
EarI CTCTTC 4 cut(s) 167, 251, 1788, 2666
EciI GGCGGA 2 cut(s) 107, 879
Ecl136II GAGCTC 3 cut(s) 240, 1450, 2554
Eco130I CCWWGG 2 cut(s) 1303, 2514
Eco24I GRGCYC 3 cut(s) 242, 1452, 2556
Eco31I GGTCTC 1 cut(s) 436
Eco32I GATATC 2 cut(s) 2198, 2746
Eco47I GGWCC 4 cut(s) 144, 1417, 1922, 2293
Eco53kI GAGCTC 3 cut(s) 240, 1450, 2554
Eco88I CYCGRG 1 cut(s) 1451
EcoICRI GAGCTC 3 cut(s) 240, 1450, 2554
EcoNI CCTNNNNNAGG 1 cut(s) 3018
EcoRI GAATTC 4 cut(s) 837, 1624, 2217, 2437
EcoRII CCWGG 4 cut(s) 188, 1371, 1419, 1585
EcoRV GATATC 2 cut(s) 2198, 2746
EcoT14I CCWWGG 2 cut(s) 1303, 2514
EcoT38I GRGCYC 3 cut(s) 242, 1452, 2556
EgeI GGCGCC 1 cut(s) 64
EheI GGCGCC 1 cut(s) 64
ErhI CCWWGG 2 cut(s) 1303, 2514
FalI AAGNNNNNCTT 6 cut(s) 164, 196, 418, 450, 716, 748
FaqI GGGAC 1 cut(s) 1935
FauI CCCGC 1 cut(s) 2078
FauNDI CATATG 1 cut(s) 466
FblI GTMKAC 2 cut(s) 795, 1545
Fnu4HI GCNGC 9 cut(s) 512, 752, 1010, 1233, 1762, 1765, 2124, 2496, 2637
FokI GGATG 6 cut(s) 313, 1499, 2113, 2621, 2777, 2957
FriOI GRGCYC 3 cut(s) 242, 1452, 2556
Fsp4HI GCNGC 9 cut(s) 512, 752, 1010, 1233, 1762, 1765, 2124, 2496, 2637
FspBI CTAG 6 cut(s) 249, 860, 1304, 1337, 1391, 1880
GlaI GCGC 3 cut(s) 64, 707, 709
GluI GCNGC 9 cut(s) 512, 752, 1010, 1233, 1762, 1765, 2124, 2496, 2637
GsaI CCCAGC 2 cut(s) 1541, 1771
GsuI CTGGAG 2 cut(s) 611, 1466
HaeII RGCGCY 1 cut(s) 66
HaeIII GGCC 7 cut(s) 344, 1090, 1226, 1989, 2183, 2888, 2912
HapII CCGG 2 cut(s) 2375, 2756
HgaI GACGC 1 cut(s) 2738
HhaI GCGC 3 cut(s) 65, 708, 710
Hin1I GRCGYC 2 cut(s) 63, 2730
Hin6I GCGC 3 cut(s) 63, 706, 708
HinP1I GCGC 3 cut(s) 63, 706, 708
HindIII AAGCTT 1 cut(s) 2428
HpaII CCGG 2 cut(s) 2375, 2756
HphI GGTGA 3 cut(s) 68, 1592, 2561
Hpy166II GTNNAC 5 cut(s) 420, 796, 943, 1546, 2623
Hpy188I TCNGA 9 cut(s) 159, 357, 490, 964, 997, 1364, 1555, 1828, 2134
Hpy8I GTNNAC 5 cut(s) 420, 796, 943, 1546, 2623
HpyAV CCTTC 6 cut(s) 256, 676, 796, 822, 1584, 2405
HpyCH4III ACNGT 8 cut(s) 646, 1086, 1165, 1267, 1298, 1503, 1666, 2668
HpyCH4IV ACGT 3 cut(s) 1701, 2952, 2998
HpyF3I CTNAG 9 cut(s) 111, 636, 1251, 1552, 1964, 2131, 2385, 2477, 2958
HpySE526I ACGT 3 cut(s) 1701, 2952, 2998
Hsp92I GRCGYC 2 cut(s) 63, 2730
HspAI GCGC 3 cut(s) 63, 706, 708
KasI GGCGCC 1 cut(s) 62
KpnI GGTACC 1 cut(s) 13
LmnI GCTCC 9 cut(s) 486, 592, 625, 745, 964, 1447, 1534, 1681, 2559
Lsp1109I GCAGC 7 cut(s) 523, 738, 1244, 1776, 2110, 2482, 2623
LweI GCATC 3 cut(s) 1047, 2135, 2742
MaeI CTAG 6 cut(s) 249, 860, 1304, 1337, 1391, 1880
MaeII ACGT 3 cut(s) 1701, 2952, 2998
MaeIII GTNAC 7 cut(s) 882, 897, 1342, 1497, 1660, 2241, 2525
MfeI CAATTG 2 cut(s) 393, 1113
MflI RGATCY 9 cut(s) 93, 114, 222, 805, 1036, 1119, 1333, 1799, 2094
MhlI GDGCHC 4 cut(s) 242, 483, 1452, 2556
MlsI TGGCCA 1 cut(s) 2888
MluNI TGGCCA 1 cut(s) 2888
Mly113I GGCGCC 1 cut(s) 63
MlyI GAGTC 3 cut(s) 262, 890, 1685
MmeI TCCRAC 2 cut(s) 182, 1849
Mox20I TGGCCA 1 cut(s) 2888
MroXI GAANNNNTTC 1 cut(s) 837
MscI TGGCCA 1 cut(s) 2888
MseI TTAA 8 cut(s) 15, 318, 756, 1025, 1043, 2091, 2357, 2367
MslI CAYNNNNRTG 2 cut(s) 567, 1322
Msp20I TGGCCA 1 cut(s) 2888
MspA1I CMGCKG 5 cut(s) 1235, 1767, 2054, 2073, 2685
MspCI CTTAAG 1 cut(s) 1042
MspI CCGG 2 cut(s) 2375, 2756
MspR9I CCNGG 4 cut(s) 190, 1373, 1421, 1587
MunI CAATTG 2 cut(s) 393, 1113
Mva1269I GAATGC 2 cut(s) 2019, 2930
MvaI CCWGG 4 cut(s) 190, 1373, 1421, 1587
MvnI CGCG 2 cut(s) 708, 2793
NarI GGCGCC 1 cut(s) 63
NcoI CCATGG 1 cut(s) 2514
NdeI CATATG 1 cut(s) 466
NmuCI GTSAC 3 cut(s) 882, 1497, 1660
NspI RCATGY 5 cut(s) 283, 656, 979, 1321, 2876
NspV TTCGAA 1 cut(s) 2081
PaeR7I CTCGAG 1 cut(s) 1451
PagI TCATGA 2 cut(s) 1992, 2466
PaqCI CACCTGC 1 cut(s) 1906
PasI CCCWGGG 1 cut(s) 1372
PauI GCGCGC 1 cut(s) 706
PciI ACATGT 1 cut(s) 652
PctI GAATGC 2 cut(s) 2019, 2930
PdmI GAANNNNTTC 1 cut(s) 837
PfeI GAWTC 9 cut(s) 352, 716, 867, 1068, 1433, 1712, 1872, 2078, 2324
PfoI TCCNGGA 1 cut(s) 1585
PkrI GCNGC 9 cut(s) 513, 753, 1011, 1234, 1763, 1766, 2125, 2497, 2638
PleI GAGTC 3 cut(s) 262, 889, 1685
PluTI GGCGCC 1 cut(s) 66
PpsI GAGTC 3 cut(s) 262, 889, 1685
PscI ACATGT 1 cut(s) 652
PsiI TTATAA 1 cut(s) 1403
Psp124BI GAGCTC 3 cut(s) 242, 1452, 2556
Psp6I CCWGG 4 cut(s) 188, 1371, 1419, 1585
PspFI CCCAGC 2 cut(s) 1537, 1767
PspGI CCWGG 4 cut(s) 188, 1371, 1419, 1585
PspPI GGNCC 5 cut(s) 144, 1417, 1922, 2181, 2293
PspXI VCTCGAGB 1 cut(s) 1451
PstI CTGCAG 2 cut(s) 1067, 2899
PsuI RGATCY 9 cut(s) 93, 114, 222, 805, 1036, 1119, 1333, 1799, 2094
PteI GCGCGC 1 cut(s) 706
PvuII CAGCTG 2 cut(s) 1235, 1767
RsaI GTAC 8 cut(s) 11, 233, 419, 944, 1021, 2452, 2877, 3025
RsaNI GTAC 8 cut(s) 10, 232, 418, 943, 1020, 2451, 2876, 3024
RseI CAYNNNNRTG 2 cut(s) 567, 1322
SacI GAGCTC 3 cut(s) 242, 1452, 2556
SaqAI TTAA 8 cut(s) 15, 318, 756, 1025, 1043, 2091, 2357, 2367
SatI GCNGC 9 cut(s) 512, 752, 1010, 1233, 1762, 1765, 2124, 2496, 2637
Sau96I GGNCC 5 cut(s) 144, 1417, 1922, 2181, 2293
SchI GAGTC 3 cut(s) 262, 890, 1685
ScrFI CCNGG 4 cut(s) 190, 1373, 1421, 1587
SduI GDGCHC 4 cut(s) 242, 483, 1452, 2556
SfaNI GCATC 3 cut(s) 1047, 2135, 2742
SfcI CTRYAG 3 cut(s) 1063, 2664, 2895
SfoI GGCGCC 1 cut(s) 64
Sfr274I CTCGAG 1 cut(s) 1451
SfuI TTCGAA 1 cut(s) 2081
SinI GGWCC 4 cut(s) 144, 1417, 1922, 2293
SlaI CTCGAG 1 cut(s) 1451
SmiMI CAYNNNNRTG 2 cut(s) 567, 1322
SmlI CTYRAG 4 cut(s) 1042, 1214, 1451, 1518
SmoI CTYRAG 4 cut(s) 1042, 1214, 1451, 1518
SspDI GGCGCC 1 cut(s) 62
SspI AATATT 2 cut(s) 2821, 2860
SspMI CTAG 6 cut(s) 249, 860, 1304, 1337, 1391, 1880
SstI GAGCTC 3 cut(s) 242, 1452, 2556
StyD4I CCNGG 4 cut(s) 188, 1371, 1419, 1585
StyI CCWWGG 2 cut(s) 1303, 2514
TaaI ACNGT 8 cut(s) 646, 1086, 1165, 1267, 1298, 1503, 1666, 2668
TaiI ACGT 3 cut(s) 1704, 2955, 3001
TauI GCSGC 2 cut(s) 1012, 1764
TfiI GAWTC 9 cut(s) 352, 716, 867, 1068, 1433, 1712, 1872, 2078, 2324
Tru1I TTAA 8 cut(s) 15, 318, 756, 1025, 1043, 2091, 2357, 2367
Tru9I TTAA 8 cut(s) 15, 318, 756, 1025, 1043, 2091, 2357, 2367
TscAI CASTG 8 cut(s) 427, 1067, 1464, 1506, 1669, 1795, 2673, 2709
TseFI GTSAC 3 cut(s) 882, 1497, 1660
TseI GCWGC 7 cut(s) 511, 751, 1232, 1764, 2123, 2495, 2636
Tsp45I GTSAC 3 cut(s) 882, 1497, 1660
TspGWI ACGGA 2 cut(s) 1951, 2557
TspRI CASTG 8 cut(s) 427, 1067, 1464, 1506, 1669, 1795, 2673, 2709
Vha464I CTTAAG 1 cut(s) 1042
VpaK11BI GGWCC 4 cut(s) 144, 1417, 1922, 2293
XagI CCTNNNNNAGG 1 cut(s) 3018
XapI RAATTY 8 cut(s) 837, 1027, 1624, 1634, 2087, 2217, 2437, 2595
XbaI TCTAGA 2 cut(s) 1336, 1879
XceI RCATGY 5 cut(s) 283, 656, 979, 1321, 2876
XcmI CCANNNNNNNNNTGG 1 cut(s) 1089
XhoI CTCGAG 1 cut(s) 1451
XmaJI CCTAGG 1 cut(s) 1303
XmiI GTMKAC 2 cut(s) 795, 1545
XmnI GAANNNNTTC 1 cut(s) 837
XspI CTAG 6 cut(s) 249, 860, 1304, 1337, 1391, 1880
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.