MD16G1120900.v1.1

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014276
Physical Location & Seq
Forward (+)
8700835 .. 8707495
6661 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1120900.v1.1.491

Sequence Viewer

Length: 3081 bp
ATGAGTTTATTCCATGTTTTTGGGTTCGGTTTAAAGGTGGGGCATCTGCTTTGGATGCTATGTTGCTGGATTGTATCTGTAATTTCCATGAACTGGTACCTCACTGGTGGGATTGTGACAGACACCAAGGTAGGTTTGCTTGGTGAAGCTGCCAATATGTGCCTCAAATGGTGGGAGAAAATCCCAATGAACATCAGCAAGATCCGCCACCATTACTACCAGTATATCGGGTCGAAGAGAGTCCGAAAAACGTGGTGGAGGAGGCTTTTGTTTTCGTGGGTGGTTGGGTGGACAATTGGGAGCCTCTGGATCTTGTGGTACATGAGCTCACAGGCCAGTGAGAAGAGGAAGGAGACTCTTGCAAGTATGTGTGATGAAAGGGCGAGGATGTTGCAGGATCAGTTTAATGTCAGCATGAACCATATCCAGGCCATGTCGATACTGATCTCAACATTCCACCACGGAAAGTATCCATCGGCCATTGATCAGAAAACTTTTGCGCGGTACACTGAAAGAACTGCTTTCGAGAGACCCCTCACAAGTGGCGTGGCATATGCTGTAAGGGTGCTCCACTCGGAAAAGGAACAATTTGAGAAGCAACAGGGCTGGACTATCAAGAGAATGGATACCCTCGAACAGAACCAGGTTCATAAGAATGACTATGCCCCAGAGGCCTTGGAACCTTCCCCAGTTCAGGAAGAATATGCTCCTGTTATCTTTGCACAGGATACCGTTAGACATGTCATCTCTTTTGATATGCTGACCGGAAAGGAAGATCGACAAAATGTGTTGCGTGCAAGAGAATCGGGAAAGGGGGTTCTTACTGCTCCTTTTCGGCTGCTTAAAACAAACAGACTTGGAGTAATACTGACATTTGCTGTCTACAAAAGAGATCTGCCCTCAAATGCAACTCCAAACGAAAGAATTCAGGCGACTGATGGGTATCTCGGTGGAATCTTTCATATTGAGTCACTAGTGGAGAAGTTACTTCAACAACTTGCTAGCAAGCAAACCATCCTTGTGGACGTGTATGATACTACCAATCAGTCGCATCCAATCAGCATGTACGGTTCAAATGTTTCAGATGATGGATTGCAGCACGTCAGCTCCTTAAGTTTTGGAGATCCCTTAAGGAACCATGAGATGCGGTGCAGGTTCAAACAGAAGCCACCTTGGCCGTGGTTAGCCATTACAACTTCTATTGGGATCCTTGTGATTGCATTGCTTGTTGGCTACATTTTCCATGCAACTGTAAATAGGATTGCCAAAGTGGAGGATGATTTCCATAAGATGATGGAACTCAAGAAACAGGCCGAGGCAGCTGATGTTGCCAAATCTCAGTTTCTTGCAACTGTTTCACACGAGATCAGAACCCCAATGAATGGTGTCCTAGGAATGTTACACATGCTAATGGACACAAACCTGGATGTTACTCAACTAGATTATGTCCGAACTGCCCAGGGAAGTGGAAAAGCTCTAGTCTCACTTATAAATGAGGTATTGGACCAGGCAAAGATTGAATCTGGTAAGCTGGAGCTTGAGGCAGTGCGTTTTGATCTGCGAGCTATTCTGGATGATGTTTTGTCACTCTTTTCGGGGAAGTCTCAACAAAAAGGAGTAGAGTTGGCAGTATACATCTCAGATCAGGTTCCTGATATGCTTATTGGTGATCCCGGGCGGTTTAGGCAGATTATCACTAATCTCATGGGGAATTCAATCAAATTCACAGAGAAAGGACACATCTTTGTGACGGTTCATCTTGTCGAGGAGCTGATCGGCTCAATAGGCGTTGAGACCGAATCATCATCCAAGAACACTTTGAGTGGTTTCCCCGTTGCAGATAAACACCACAGTTGGGGAGGATTTAGGTGTTTCGGTCAAGATGGATCTGCAAGCCGTTTTTCATCCTCATCTGACCTCATAAATATTATTGTATCTGTTGAGGACACAGGAGTAGGAATCCCTCTAGAAGCCCAATCTCGTGTTTTCACTCCTTTTATGCAGGTGGGACCATCCATCTCACGTACACATGGAGGCACAGGAATTGGACTAAGCATAAGCAAGTGTTTGGTTGGCCTCATGAAAGGGGAAATCGGGTTTGTTAGCATTCCGAAGATTGGTTCCACATTTACATTTACTGCTGTTTTCACCAATGCCAGCTCCAGTTCAAATGAGTTAACCATCGAGCAAATCAACAGCCAGTCAAATGCTGCATCCTCAGAATTTAATGGCATGACGGCATTAGTAGTGGACCAAAGACCTGTTAGGGCCAAGATGTCAAGTTATCATATCGAACGGCTTGGAATTCGTGTTGAAGTAGTTTCTGATTTGAATCAGGGTTTAGCTAGCATAAGCTGTGGGAGTACAACTATCAATATGGTCCTTGTTGAACAGGAAGTCTGGAATAAAGATTCTGGAACTTCAGCTCTCTTTGTCAGTAATTTACCGAAAATTGATGGACGGGTTCCTCCAAAACTGTTCATTCTTGCCAATTCTAGTAGCTCATGCAGAATAAGCTCTGCAACTTCAGGTGTCTCTACCCGAACCGTTATCATGAAACCGCTCAGGGCAAGCATGCTCGCTGCCTCACTGCAAAGAGCCATGGGAGTTGGTAACAAGGGAAATCTCCGGAATGGGGAGCTCCCAAGTTTGTCTCTCCGCAATCTTCTGCTCAGAAGGAAAATTCTTATTATAGATGATAATAATGTGAATCTCAGAGTAGCTGCCGGTGCTTTGAAAAAGTATGGGGCAGAGGTGATCTGTGCAGACAGTGGTAAAAAGGCAATCTCACTGCTTACGCCGCCCCACCATTTTGATGCCTGCTTCATGGATATCCAGATGCCAGAGATGGATGGGTTTGAAGCTACAAGGAGAATACGTGACTTAGAACGCAATATCAGCAACAGTATTCAAGCATGGCATGTACCCATTCTGGCCATGACTGCTGATGTAATTCAGGCAACCCATGAGGAATGCACAAAGTGCGGAATGGATGGATATGTTTCAAAACCATTTGAAGCTGAACAGCTTTATCGCGAAGTTTCACGTTTTTTCCAATCTACTACAACTGGAAACGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009743 GO:0009744 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0009991 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010150 GO:0010271 GO:0010959 GO:0012505 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034285 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042175 GO:0042221 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048366 GO:0048367 GO:0048509 GO:0048519 GO:0048580 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071396 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0090693 GO:0097305 GO:0097306 GO:0098542 GO:0098827 GO:0099402 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1027

Amino Acids

114.13

Weight (kDa)

8.56

Isoelectric Point (pI)

42.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 160 - 356 5.3e-38 CHASE domain
HisKA PF00512 444 - 509 4.3e-18 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 642 - 714 3.7e-14 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 739 - 869 2.6e-42 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 895 - 1013 1.5e-22 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27320
fragaria_vesca FvH4_4g23590 FvH4_4g23590 FvH4_4g23590 FvH4_4g23590
malus_domestica MD13G1120900.v1.1 MD16G1068400.v1.1 MD16G1120900.v1.1
prunus_persica Prupe.1G224300_v2.0.a1
pyrus_communis pycom13g10520
rosa_chinensis RchiOBHm_Chr4g0430011
rosa_laevigata RLG00000007014
rosa_roxburghii Rroxscaffold_5G00371820
rosa_rugosa Rorug04G0239800
rosa_samantha Rh4BG302900 Rh4CG318400
rosa_wichuraiana Rw4G025680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1490
AarI CACCTGC 1 cut(s) 1993
Acc36I ACCTGC 2 cut(s) 1143, 1993
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 2 cut(s) 93, 1382
AccBSI CCGCTC 1 cut(s) 2563
AccI GTMKAC 2 cut(s) 882, 1632
AccII CGCG 3 cut(s) 502, 3036, 3077
AccIII TCCGGA 1 cut(s) 2628
AciI CCGC 8 cut(s) 205, 502, 1147, 1678, 2561, 2659, 2801, 2985
AclWI GGATC 8 cut(s) 196, 317, 405, 1118, 1201, 1214, 1664, 1894
AcoI YGGCCR 3 cut(s) 477, 1175, 2934
AcsI RAATTY 6 cut(s) 924, 1711, 1721, 2222, 2304, 2682
AcuI CTGAAG 2 cut(s) 2406, 2511
AdeI CACNNNGTG 1 cut(s) 2982
AfaI GTAC 7 cut(s) 98, 320, 506, 1067, 2026, 2365, 2925
AfiI CCNNNNNNNGG 7 cut(s) 93, 427, 694, 1382, 1855, 2117, 2635
AflII CTTAAG 2 cut(s) 1111, 1129
AflIII ACRYGT 3 cut(s) 739, 1026, 3075
AhlI ACTAGT 1 cut(s) 973
AjiI CACGTC 2 cut(s) 1027, 1102
AjnI CCWGG 5 cut(s) 426, 642, 1422, 1458, 1506
AloI GAACNNNNNNTCC 2 cut(s) 801, 833
Alw21I GWGCWC 3 cut(s) 329, 570, 2643
Alw26I GTCTC 7 cut(s) 347, 523, 1486, 1608, 1787, 2539, 2658
AlwI GGATC 8 cut(s) 196, 317, 405, 1118, 1201, 1214, 1664, 1894
Ama87I CYCGRG 1 cut(s) 1673
Aor13HI TCCGGA 1 cut(s) 2628
AoxI GGCC 9 cut(s) 333, 429, 477, 672, 1175, 1311, 2074, 2268, 2934
ApeKI GCWGC 7 cut(s) 149, 838, 1096, 1319, 2210, 2582, 2723
ApoI RAATTY 6 cut(s) 924, 1711, 1721, 2222, 2304, 2682
Asp700I GAANNNNTTC 1 cut(s) 924
Asp718I GGTACC 1 cut(s) 96
AspA2I CCTAGG 1 cut(s) 1390
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 5 cut(s) 1504, 2009, 2251, 2268, 2380
AsuC2I CCSGG 2 cut(s) 1674, 1675
AsuHPI GGTGA 4 cut(s) 155, 1679, 2140, 2767
AsuNHI GCTAGC 2 cut(s) 1001, 2345
AvaI CYCGRG 1 cut(s) 1673
AvaII GGWCC 4 cut(s) 1504, 2009, 2251, 2380
AvrII CCTAGG 1 cut(s) 1390
BalI TGGCCA 1 cut(s) 2936
BamHI GGATCC 1 cut(s) 1206
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 2 cut(s) 329, 2643
BauI CACGAG 2 cut(s) 1361, 1980
Bbv12I GWGCWC 3 cut(s) 329, 570, 2643
BbvI GCAGC 7 cut(s) 136, 825, 1108, 1331, 2197, 2569, 2710
BceAI ACGGC 4 cut(s) 1162, 1881, 2253, 2312
BcgI CGANNNNNNTGC 4 cut(s) 373, 407, 786, 820
BciT130I CCWGG 5 cut(s) 428, 644, 1424, 1460, 1508
BciVI GTATCC 3 cut(s) 480, 619, 721
BclI TGATCA 1 cut(s) 484
BcnI CCSGG 2 cut(s) 1674, 1675
BcoDI GTCTC 7 cut(s) 347, 523, 1486, 1608, 1787, 2539, 2658
BcuI ACTAGT 1 cut(s) 973
BfaI CTAG 8 cut(s) 974, 1002, 1391, 1439, 1478, 1967, 2346, 2496
BfrI CTTAAG 2 cut(s) 1111, 1129
BfuAI ACCTGC 2 cut(s) 1143, 1993
BfuI GTATCC 3 cut(s) 480, 619, 721
BglI GCCNNNNNGGC 2 cut(s) 671, 1174
BglII AGATCT 1 cut(s) 892
BisI GCNGC 8 cut(s) 150, 839, 1097, 1320, 2211, 2583, 2724, 2801
BlnI CCTAGG 1 cut(s) 1390
BlsI GCNGC 8 cut(s) 151, 840, 1098, 1321, 2212, 2584, 2725, 2802
Bme1390I CCNGG 7 cut(s) 428, 644, 1424, 1460, 1508, 1674, 1675
Bme18I GGWCC 4 cut(s) 1504, 2009, 2251, 2380
BmeT110I CYCGRG 1 cut(s) 1673
BmgBI CACGTC 2 cut(s) 1027, 1102
BmgT120I GGNCC 5 cut(s) 1504, 2009, 2251, 2268, 2380
BmiI GGNNCC 9 cut(s) 98, 302, 681, 1136, 1208, 1650, 2010, 2122, 2466
BmrFI CCNGG 7 cut(s) 428, 644, 1424, 1460, 1508, 1674, 1675
BmrI ACTGGG 1 cut(s) 683
BmsI GCATC 7 cut(s) 45, 52, 1060, 1134, 2222, 2806, 2829
BmtI GCTAGC 2 cut(s) 1005, 2349
BmuI ACTGGG 1 cut(s) 683
BpmI CTGGAG 2 cut(s) 1553, 2146
Bpu10I CCTNAGC 1 cut(s) 2564
BpuEI CTTGAG 2 cut(s) 1286, 1559
BpuMI CCSGG 2 cut(s) 1674, 1675
BsaAI YACGTR 2 cut(s) 2024, 2879
BsaBI GATNNNNATC 3 cut(s) 443, 942, 2331
BsaI GGTCTC 2 cut(s) 523, 1787
BsaWI WCCGGW 2 cut(s) 764, 2628
BsaXI ACNNNNNCTCC 4 cut(s) 1091, 1121, 2863, 2893
Bsc4I CCNNNNNNNGG 7 cut(s) 93, 427, 694, 1382, 1855, 2117, 2635
Bse118I RCCGGY 1 cut(s) 2726
Bse1I ACTGG 8 cut(s) 98, 109, 220, 336, 689, 2163, 2200, 3073
Bse3DI GCAATG 1 cut(s) 1220
Bse8I GATNNNNATC 3 cut(s) 443, 942, 2331
BseAI TCCGGA 1 cut(s) 2628
BseBI CCWGG 5 cut(s) 428, 644, 1424, 1460, 1508
BseJI GATNNNNATC 3 cut(s) 443, 942, 2331
BseLI CCNNNNNNNGG 7 cut(s) 93, 427, 694, 1382, 1855, 2117, 2635
BseMI GCAATG 1 cut(s) 1220
BseMII CTCAG 6 cut(s) 1352, 1653, 2232, 2578, 2686, 2728
BseNI ACTGG 8 cut(s) 98, 109, 220, 336, 689, 2163, 2200, 3073
BseRI GAGGAG 2 cut(s) 274, 1781
BseXI GCAGC 7 cut(s) 136, 825, 1108, 1331, 2197, 2569, 2710
BsgI GTGCAG 2 cut(s) 1171, 2784
Bsh1236I CGCG 3 cut(s) 502, 3036, 3077
BshFI GGCC 9 cut(s) 335, 431, 479, 674, 1177, 1313, 2076, 2270, 2936
BshNI GGYRCC 1 cut(s) 96
BsiHKAI GWGCWC 3 cut(s) 329, 570, 2643
BsiHKCI CYCGRG 1 cut(s) 1673
BsiSI CCGG 4 cut(s) 765, 1674, 2629, 2727
BslFI GGGAC 1 cut(s) 2022
BslI CCNNNNNNNGG 7 cut(s) 93, 427, 694, 1382, 1855, 2117, 2635
BsmAI GTCTC 7 cut(s) 347, 523, 1486, 1608, 1787, 2539, 2658
BsmFI GGGAC 1 cut(s) 2022
BsmI GAATGC 2 cut(s) 2106, 2978
BsnI GGCC 9 cut(s) 335, 431, 479, 674, 1177, 1313, 2076, 2270, 2936
Bso31I GGTCTC 2 cut(s) 523, 1787
BsoBI CYCGRG 1 cut(s) 1673
Bsp1286I GDGCHC 3 cut(s) 329, 570, 2643
Bsp13I TCCGGA 1 cut(s) 2628
Bsp19I CCATGG 1 cut(s) 2601
Bsp68I TCGCGA 1 cut(s) 3036
BspACI CCGC 8 cut(s) 205, 502, 1147, 1678, 2561, 2659, 2801, 2985
BspANI GGCC 9 cut(s) 335, 431, 479, 674, 1177, 1313, 2076, 2270, 2936
BspCNI CTCAG 6 cut(s) 1351, 1652, 2231, 2577, 2685, 2727
BspEI TCCGGA 1 cut(s) 2628
BspFNI CGCG 3 cut(s) 502, 3036, 3077
BspHI TCATGA 2 cut(s) 2079, 2553
BspLI GGNNCC 9 cut(s) 98, 302, 681, 1136, 1208, 1650, 2010, 2122, 2466
BspMI ACCTGC 2 cut(s) 1143, 1993
BspOI GCTAGC 2 cut(s) 1005, 2349
BspPI GGATC 8 cut(s) 196, 317, 405, 1118, 1201, 1214, 1664, 1894
BspT107I GGYRCC 1 cut(s) 96
BspTI CTTAAG 2 cut(s) 1111, 1129
BspTNI GGTCTC 2 cut(s) 523, 1787
BsrBI CCGCTC 1 cut(s) 2563
BsrDI GCAATG 1 cut(s) 1220
BsrFI RCCGGY 1 cut(s) 2726
BsrI ACTGG 8 cut(s) 98, 109, 220, 336, 689, 2163, 2200, 3073
BssAI RCCGGY 1 cut(s) 2726
BssNAI GTATAC 1 cut(s) 1633
BssSI CACGAG 2 cut(s) 1361, 1980
BssT1I CCWWGG 5 cut(s) 126, 675, 1172, 1390, 2601
Bst1107I GTATAC 1 cut(s) 1633
Bst2BI CACGAG 2 cut(s) 1361, 1980
Bst2UI CCWGG 5 cut(s) 428, 644, 1424, 1460, 1508
Bst6I CTCTTC 2 cut(s) 230, 338
BstAFI CTTAAG 2 cut(s) 1111, 1129
BstAPI GCANNNNNTGC 1 cut(s) 2982
BstBAI YACGTR 2 cut(s) 2024, 2879
BstDEI CTNAG 8 cut(s) 1338, 1639, 2051, 2218, 2564, 2672, 2714, 2884
BstDSI CCRYGG 3 cut(s) 460, 1178, 2601
BstFNI CGCG 3 cut(s) 502, 3036, 3077
BstHHI GCGC 1 cut(s) 502
BstMAI GTCTC 7 cut(s) 347, 523, 1486, 1608, 1787, 2539, 2658
BstNI CCWGG 5 cut(s) 428, 644, 1424, 1460, 1508
BstNSI RCATGY 5 cut(s) 743, 1066, 1408, 2578, 2924
BstSCI CCNGG 7 cut(s) 426, 642, 1422, 1458, 1506, 1672, 1673
BstUI CGCG 3 cut(s) 502, 3036, 3077
BstV1I GCAGC 7 cut(s) 136, 825, 1108, 1331, 2197, 2569, 2710
BstX2I RGATCY 6 cut(s) 201, 309, 892, 1123, 1206, 1886
BstXI CCANNNNNNTGG 3 cut(s) 20, 1021, 1466
BstYI RGATCY 6 cut(s) 201, 309, 892, 1123, 1206, 1886
BstZ17I GTATAC 1 cut(s) 1633
BsuI GTATCC 3 cut(s) 480, 619, 721
BsuRI GGCC 9 cut(s) 335, 431, 479, 674, 1177, 1313, 2076, 2270, 2936
BtgI CCRYGG 3 cut(s) 460, 1178, 2601
BtrI CACGTC 2 cut(s) 1027, 1102
BtsI GCAGTG 3 cut(s) 1551, 2588, 2789
BtsIMutI CAGTG 7 cut(s) 102, 343, 507, 1551, 2588, 2776, 2789
BtuMI TCGCGA 1 cut(s) 3036
BveI ACCTGC 2 cut(s) 1143, 1993
CciI TCATGA 2 cut(s) 2079, 2553
CfoI GCGC 1 cut(s) 502
Cfr10I RCCGGY 1 cut(s) 2726
Cfr13I GGNCC 5 cut(s) 1504, 2009, 2251, 2268, 2380
Cfr9I CCCGGG 1 cut(s) 1673
CsiI ACCWGGT 1 cut(s) 642
Csp6I GTAC 7 cut(s) 97, 319, 505, 1066, 2025, 2364, 2924
CspCI CAANNNNNGTGG 2 cut(s) 528, 563
CviQI GTAC 7 cut(s) 97, 319, 505, 1066, 2025, 2364, 2924
DdeI CTNAG 8 cut(s) 1338, 1639, 2051, 2218, 2564, 2672, 2714, 2884
DraI TTTAAA 1 cut(s) 33
DraIII CACNNNGTG 1 cut(s) 2982
EaeI YGGCCR 3 cut(s) 477, 1175, 2934
Eam1104I CTCTTC 2 cut(s) 230, 338
EarI CTCTTC 2 cut(s) 230, 338
EciI GGCGGA 1 cut(s) 194
Ecl136II GAGCTC 2 cut(s) 327, 2641
Eco130I CCWWGG 5 cut(s) 126, 675, 1172, 1390, 2601
Eco147I AGGCCT 1 cut(s) 674
Eco24I GRGCYC 2 cut(s) 329, 2643
Eco31I GGTCTC 2 cut(s) 523, 1787
Eco32I GATATC 1 cut(s) 2833
Eco47I GGWCC 4 cut(s) 1504, 2009, 2251, 2380
Eco53kI GAGCTC 2 cut(s) 327, 2641
Eco57I CTGAAG 2 cut(s) 2406, 2511
Eco88I CYCGRG 1 cut(s) 1673
EcoICRI GAGCTC 2 cut(s) 327, 2641
EcoRI GAATTC 3 cut(s) 924, 1711, 2304
EcoRII CCWGG 5 cut(s) 426, 642, 1422, 1458, 1506
EcoRV GATATC 1 cut(s) 2833
EcoT14I CCWWGG 5 cut(s) 126, 675, 1172, 1390, 2601
EcoT38I GRGCYC 2 cut(s) 329, 2643
ErhI CCWWGG 5 cut(s) 126, 675, 1172, 1390, 2601
FalI AAGNNNNNCTT 2 cut(s) 505, 537
FaqI GGGAC 1 cut(s) 2022
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 1 cut(s) 484
FblI GTMKAC 2 cut(s) 882, 1632
Fnu4HI GCNGC 8 cut(s) 150, 839, 1097, 1320, 2211, 2583, 2724, 2801
FriOI GRGCYC 2 cut(s) 329, 2643
Fsp4HI GCNGC 8 cut(s) 150, 839, 1097, 1320, 2211, 2583, 2724, 2801
FspBI CTAG 8 cut(s) 974, 1002, 1391, 1439, 1478, 1967, 2346, 2496
GlaI GCGC 1 cut(s) 501
GluI GCNGC 8 cut(s) 150, 839, 1097, 1320, 2211, 2583, 2724, 2801
GsuI CTGGAG 2 cut(s) 1553, 2146
HaeIII GGCC 9 cut(s) 335, 431, 479, 674, 1177, 1313, 2076, 2270, 2936
HapII CCGG 4 cut(s) 765, 1674, 2629, 2727
HhaI GCGC 1 cut(s) 502
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 1 cut(s) 2178
HindII GTYRAC 1 cut(s) 2178
HpaI GTTAAC 1 cut(s) 2178
HpaII CCGG 4 cut(s) 765, 1674, 2629, 2727
HphI GGTGA 4 cut(s) 155, 1679, 2140, 2767
Hpy166II GTNNAC 8 cut(s) 291, 507, 883, 1024, 1633, 2027, 2178, 2251
Hpy8I GTNNAC 8 cut(s) 291, 507, 883, 1024, 1633, 2027, 2178, 2251
HpyAV CCTTC 3 cut(s) 343, 693, 2670
HpyCH4IV ACGT 6 cut(s) 251, 1026, 1101, 2023, 2878, 3046
HpyF3I CTNAG 8 cut(s) 1338, 1639, 2051, 2218, 2564, 2672, 2714, 2884
HpySE526I ACGT 6 cut(s) 251, 1026, 1101, 2023, 2878, 3046
HspAI GCGC 1 cut(s) 500
Kpn2I TCCGGA 1 cut(s) 2628
KpnI GGTACC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 484
KspAI GTTAAC 1 cut(s) 2178
Lsp1109I GCAGC 7 cut(s) 136, 825, 1108, 1331, 2197, 2569, 2710
LweI GCATC 7 cut(s) 45, 52, 1060, 1134, 2222, 2806, 2829
MabI ACCWGGT 1 cut(s) 642
MaeI CTAG 8 cut(s) 974, 1002, 1391, 1439, 1478, 1967, 2346, 2496
MaeII ACGT 6 cut(s) 251, 1026, 1101, 2023, 2878, 3046
MaeIII GTNAC 9 cut(s) 115, 969, 984, 1398, 1429, 1584, 1747, 2612, 2879
MbiI CCGCTC 1 cut(s) 2563
MboII GAAGA 6 cut(s) 247, 355, 710, 785, 2125, 2657
MfeI CAATTG 1 cut(s) 294
MflI RGATCY 6 cut(s) 201, 309, 892, 1123, 1206, 1886
MhlI GDGCHC 3 cut(s) 329, 570, 2643
MlsI TGGCCA 1 cut(s) 2936
MluI ACGCGT 1 cut(s) 3075
MluNI TGGCCA 1 cut(s) 2936
MlyI GAGTC 3 cut(s) 249, 349, 977
Mox20I TGGCCA 1 cut(s) 2936
MroI TCCGGA 1 cut(s) 2628
MroXI GAANNNNTTC 1 cut(s) 924
MscI TGGCCA 1 cut(s) 2936
MseI TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2177, 2226
MslI CAYNNNNRTG 5 cut(s) 654, 1019, 1409, 1745, 2814
Msp20I TGGCCA 1 cut(s) 2936
MspA1I CMGCKG 1 cut(s) 1322
MspCI CTTAAG 2 cut(s) 1111, 1129
MspI CCGG 4 cut(s) 765, 1674, 2629, 2727
MspR9I CCNGG 7 cut(s) 428, 644, 1424, 1460, 1508, 1674, 1675
MunI CAATTG 1 cut(s) 294
Mva1269I GAATGC 2 cut(s) 2106, 2978
MvaI CCWGG 5 cut(s) 428, 644, 1424, 1460, 1508
MvnI CGCG 3 cut(s) 502, 3036, 3077
NciI CCSGG 2 cut(s) 1674, 1675
NcoI CCATGG 1 cut(s) 2601
NdeI CATATG 1 cut(s) 553
NheI GCTAGC 2 cut(s) 1001, 2345
NlaIV GGNNCC 9 cut(s) 98, 302, 681, 1136, 1208, 1650, 2010, 2122, 2466
NmeAIII GCCGAG 1 cut(s) 1339
NmuCI GTSAC 5 cut(s) 115, 969, 1584, 1747, 2879
NruI TCGCGA 1 cut(s) 3036
NspI RCATGY 5 cut(s) 743, 1066, 1408, 2578, 2924
PaeI GCATGC 1 cut(s) 2578
PagI TCATGA 2 cut(s) 2079, 2553
PaqCI CACCTGC 1 cut(s) 1993
PasI CCCWGGG 1 cut(s) 1459
PceI AGGCCT 1 cut(s) 674
PciI ACATGT 1 cut(s) 739
PctI GAATGC 2 cut(s) 2106, 2978
PdmI GAANNNNTTC 1 cut(s) 924
PfeI GAWTC 8 cut(s) 803, 954, 1520, 1799, 1959, 2332, 2411, 2710
PflMI CCANNNNNTGG 2 cut(s) 93, 1382
PkrI GCNGC 8 cut(s) 151, 840, 1098, 1321, 2212, 2584, 2725, 2802
PleI GAGTC 3 cut(s) 248, 349, 976
PpsI GAGTC 3 cut(s) 248, 349, 976
Ppu21I YACGTR 2 cut(s) 2024, 2879
PscI ACATGT 1 cut(s) 739
PsiI TTATAA 1 cut(s) 1490
Psp124BI GAGCTC 2 cut(s) 329, 2643
Psp6I CCWGG 5 cut(s) 426, 642, 1422, 1458, 1506
PspGI CCWGG 5 cut(s) 426, 642, 1422, 1458, 1506
PspN4I GGNNCC 9 cut(s) 98, 302, 681, 1136, 1208, 1650, 2010, 2122, 2466
PspPI GGNCC 5 cut(s) 1504, 2009, 2251, 2268, 2380
PsuI RGATCY 6 cut(s) 201, 309, 892, 1123, 1206, 1886
PvuII CAGCTG 1 cut(s) 1322
RruI TCGCGA 1 cut(s) 3036
RsaI GTAC 7 cut(s) 98, 320, 506, 1067, 2026, 2365, 2925
RsaNI GTAC 7 cut(s) 97, 319, 505, 1066, 2025, 2364, 2924
RseI CAYNNNNRTG 5 cut(s) 654, 1019, 1409, 1745, 2814
SacI GAGCTC 2 cut(s) 329, 2643
SaqAI TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2177, 2226
SatI GCNGC 8 cut(s) 150, 839, 1097, 1320, 2211, 2583, 2724, 2801
Sau96I GGNCC 5 cut(s) 1504, 2009, 2251, 2268, 2380
SchI GAGTC 3 cut(s) 249, 349, 977
ScrFI CCNGG 7 cut(s) 428, 644, 1424, 1460, 1508, 1674, 1675
SduI GDGCHC 3 cut(s) 329, 570, 2643
SexAI ACCWGGT 1 cut(s) 642
SfaNI GCATC 7 cut(s) 45, 52, 1060, 1134, 2222, 2806, 2829
SinI GGWCC 4 cut(s) 1504, 2009, 2251, 2380
SmaI CCCGGG 1 cut(s) 1675
SmiMI CAYNNNNRTG 5 cut(s) 654, 1019, 1409, 1745, 2814
SmlI CTYRAG 4 cut(s) 1111, 1129, 1301, 1538
SmoI CTYRAG 4 cut(s) 1111, 1129, 1301, 1538
SpeI ACTAGT 1 cut(s) 973
SphI GCATGC 1 cut(s) 2578
SseBI AGGCCT 1 cut(s) 674
SsiI CCGC 8 cut(s) 205, 502, 1147, 1678, 2561, 2659, 2801, 2985
SspI AATATT 1 cut(s) 1927
SspMI CTAG 8 cut(s) 974, 1002, 1391, 1439, 1478, 1967, 2346, 2496
SstI GAGCTC 2 cut(s) 329, 2643
StuI AGGCCT 1 cut(s) 674
StyD4I CCNGG 7 cut(s) 426, 642, 1422, 1458, 1506, 1672, 1673
StyI CCWWGG 5 cut(s) 126, 675, 1172, 1390, 2601
TaiI ACGT 6 cut(s) 254, 1029, 1104, 2026, 2881, 3049
TaqI TCGA 8 cut(s) 233, 437, 525, 633, 778, 1764, 2184, 2292
TaqII GACCGA 2 cut(s) 1811, 1865
TatI WGTACW 1 cut(s) 2363
TauI GCSGC 1 cut(s) 2803
TfiI GAWTC 8 cut(s) 803, 954, 1520, 1799, 1959, 2332, 2411, 2710
Tru1I TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2177, 2226
Tru9I TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2177, 2226
TscAI CASTG 7 cut(s) 109, 343, 514, 1551, 2595, 2776, 2796
TseFI GTSAC 5 cut(s) 115, 969, 1584, 1747, 2879
TseI GCWGC 7 cut(s) 149, 838, 1096, 1319, 2210, 2582, 2723
Tsp45I GTSAC 5 cut(s) 115, 969, 1584, 1747, 2879
TspGWI ACGGA 1 cut(s) 477
TspMI CCCGGG 1 cut(s) 1673
TspRI CASTG 7 cut(s) 109, 343, 514, 1551, 2595, 2776, 2796
Van91I CCANNNNNTGG 2 cut(s) 93, 1382
Vha464I CTTAAG 2 cut(s) 1111, 1129
VpaK11BI GGWCC 4 cut(s) 1504, 2009, 2251, 2380
XapI RAATTY 6 cut(s) 924, 1711, 1721, 2222, 2304, 2682
XbaI TCTAGA 1 cut(s) 1966
XceI RCATGY 5 cut(s) 743, 1066, 1408, 2578, 2924
XcmI CCANNNNNNNNNTGG 1 cut(s) 1176
XmaI CCCGGG 1 cut(s) 1673
XmaJI CCTAGG 1 cut(s) 1390
XmiI GTMKAC 2 cut(s) 882, 1632
XmnI GAANNNNTTC 1 cut(s) 924
XspI CTAG 8 cut(s) 974, 1002, 1391, 1439, 1478, 1967, 2346, 2496
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.