Rh4BG302900

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014276
Physical Location & Seq
Reverse (-)
47345339 .. 47350408
5070 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG302900.1

Sequence Viewer

Length: 3117 bp
ATGAGTTTGCTTCATGTCTTCGGGTTCGGTTTAAAAGTGGGGCATCTACTTTGGATGCTATGTTGCTGGATTGTATCTGTGATTTCCATGAATTGGTACCTTAATGGTGCAATTATGGACACCAAGAGTGGTAGTTTGCTTGGTGATGGCGCCCGCGCCCTCAAATTGTGGGAGAAGATCCCATTGAACATTACTAAGATCCGCCATCATTACTACCAGTATATTGGGTCCAAGAGGGTTCCGAAGACTTGGTGGAAGAGGGTCTTGGTTTCCTGGGTGGTTGGTTCGACTATTGCTTGCTTTTGGATCTTCTGCTACATGAGCTCAGTGGCTAGTGAGAAGAGGAAAGAGACTCTTACGAGTATGTGTGATGAGAGAGCAAGGATGTTGCAGGATCAGTTTAATGTGAGCATGAACCATATTCAGGCCATGTCGATTCTGATCTCCACATTCCACCACGGCAAGTATCCATCTGCAATTGACCAGAGAACTTTTGCGAGGTACACTGAAAGAACTGCTTTTGAGAGACCTCTTACAAGTGGCGTGGCATATGCTGTAAGGGTGCTCCACTCGGAGAAAGAACAATTTGAGAAGCAGCAAGGCTGGACTATCAAGAGAATGGATACCCTCGAACACGACCAAGTCCATAAGAATGACTATACCCCAGAATTGCTGGAGCCACCCCCAATTCAGGAAGAATATGCTCCTGTCATATTTGCTCAGGATACTGTTGCACATGTCATTTCTTTCGATATGCTGTCTGGGAAGGAAGATCGAGATAATGTGTTGCGCGCAAGAGAATCAGGAAAGGGGGTCTTGACTGCTCCATTTCGGCTGCTTAAAACTAATAGGCTTGGAGTAATACTGACCTTTGCTGTCTACAAGAGAGATCTACCTTCAAATGCAACTCCAAATGAAAGAATTCAAGCAACCGATGGGTATCTAGGCGGAATCTTTCATATTGAGTCACTTGTGGAGAAGTTACTTCAACAACTTGCAAGCAAGCAAACTATCCTTGTCAATGTGTATGATACTACCAATCATTCGGAGCCAATCAGCATGTATGGTTCAAATGTTTCAGATGATGGATTGCGGCATATTAGTACCTTAAATTTTGGAGATCCCTTAAGGAGGCATGAGATGCACTGCAGATTCAAACATAAGCCACCGTGGCCGTGGTTAGCCATCACAACTTCAATTGGGATCCTTGTGATTGTGTTGCTTGTTGGGCATATTTTTCATGCAACTGTAAATAGGATTGCCAAAGTGGAAGATGATTTCCATAAAATGAGCGATCTCAAGAAACAGGCCGAAGCAGCTGATGTTGCCAAATCTCAGTTCCTTGCAACTGTATCCCATGAGATTAGAACCCCAATGAACGGTGTCCTAGGTATGTTGCACATGCTTATGGATACAGATCTAGATGTAACTCAACAAGATTATGTCAGAACTGCCCAGGGTAGTGGAAAAGCCCTAGTCTCACTTATAAATGAGGTCTTGGACCAGGCAAAAATTGAATCTGGTAAGCTGGAGCTCGAGGCAGTGCGTTTTGATCTGCGAGCAATTTTGGATGATGTTTTGTCACTGTTTTCTGGGAAGTCTCAAGAAAAAGGAGTGGAGCTGGGAGTATACATCTCAGATCAGGTTCCTGAAATGCTTATTGGTGATCCTGGAAGGTTTCGGCAAATTATTACCAATCTCATGGGGAATTCGATCAAATTCACAGAGAAAGGACACATCTTTGTCACTGTCCATTTGGTTGAGGAGCTGATTGACTCGATAGACGTTGAGACTGAATCATCATCGAAAAACACTTTGAGTGGGTTCCCTGTTGCGGATAAACGCCGCAGCTGGGAAGGATTTAGGGGTTTCAGTGAAGAGGGATCTGCAAGCTCGTTCTCATCGTCGTCTGATGTCATCAATCTTATTGTGTCTGTTGAGGACACAGGGGTTGGAATCCCTCTAGAAGCCCAATCTCGTGTTTTCACTCCTTTTATGCAGGTGGGACCATCTATCTCACGGACACATGGTGGCACAGGAATTGGACTAAGCATAAGCAAGTGTTTAGTTGGCCTCATGAGAGGGGAGATTGGTTTTGTGAGCATTCCCAAGGTTGGTTCCACATTTACATTTACCGCTGTTTTCGCCAAAGCCCGCTGTGATTCGAATGAATTTAAGATCCAGCAAATCAACAGCCAAGCAAATGCTGCATCCTCAGACTTTCATGGCATGACAGCGTTGGTTGTGGATCACAGACCTGTGAGGGCCAAGATGTCAAGATATCATATCCAAAGGCTTGGAATTCGTGTTGAAGTCGCTTCTGAGTTGCAGCAGGCTTTGTCTTGCATAAGCTCTGGGAATACAACTATAAATATGGTCCTTGTTGAACAGGAGGTTTGGGATAATGATTCACGCAGTTCAGCTCTCTTTATTGATAACTTAAAGAAACTTAAATGTCCGGTTCCTCCTAAGCTGTTCCTTCTTGCCAATTCTATAAGCAGTTGCAGAACAAGCTTTGTGAATTCAGGTGTCTGTACGCCAAGTATTATCATGAAGCCTCTCAGGGCAAGTATGCTTGCTGCCTCACTACAAAGAGCCATGGGAATTGGTAACAAGGGTAATACACGGAATGGTGAGCTCCCAAGTTTGTCTCTCCGCAATCTTCTACTTGGAAGAAAAATTCTTATTGTGGATGATAATAAAGTGAACCTCATAGTAGCTGCTGGTGCTTTGAAAAAGTATGGGGCTACAGTGAAGAGTGCAGACAGCGGTAAAGAGGCAATCTCACTGCTTACACCACCCCATAGTTTTGATGCCTGCTTCATGGATATCCAGATGCCGGAAATGGATGGTTTTGAAGCTACGAGGAGAATACGCGACATAGAACGCAATGTCAGCAATAATATTCAACATGGCAAGGTTTCTGCGGAGGACTATGACAATATTTTATCATGGCATGTACCCATTCTGGCCATGACTGCAGATGTTATTCAGGCCACCCATGAGGAATGCACAAGGTGTGGAATGGATGGATATGTTTCTAAACCATTTGAAGCTGAACAGCTTTATCGAGAAGTTTCACGTTTTCTCCATTCTCCTGCAAAAGGTACTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009743 GO:0009744 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0009991 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010150 GO:0010271 GO:0010959 GO:0012505 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034285 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042175 GO:0042221 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048366 GO:0048367 GO:0048509 GO:0048519 GO:0048580 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071396 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0090693 GO:0097305 GO:0097306 GO:0098542 GO:0098827 GO:0099402 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1038

Amino Acids

115.71

Weight (kDa)

7.86

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 159 - 355 7.5e-38 CHASE domain
HisKA PF00512 443 - 508 4.4e-19 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 641 - 712 2.7e-14 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 738 - 868 4.2e-44 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 894 - 1025 1.3e-19 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27320
fragaria_vesca FvH4_4g23590 FvH4_4g23590 FvH4_4g23590 FvH4_4g23590
malus_domestica MD13G1120900.v1.1 MD16G1068400.v1.1 MD16G1120900.v1.1
prunus_persica Prupe.1G224300_v2.0.a1
pyrus_communis pycom13g10520
rosa_chinensis RchiOBHm_Chr4g0430011
rosa_laevigata RLG00000007014
rosa_roxburghii Rroxscaffold_5G00371820
rosa_rugosa Rorug04G0239800
rosa_samantha Rh4BG302900 Rh4CG318400
rosa_wichuraiana Rw4G025680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1487
AarI CACCTGC 1 cut(s) 1990
Acc36I ACCTGC 1 cut(s) 1990
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 2 cut(s) 96, 149
AccB7I CCANNNNNTGG 1 cut(s) 93
AccI GTMKAC 2 cut(s) 879, 1629
AccII CGCG 3 cut(s) 156, 792, 2877
AcoI YGGCCR 2 cut(s) 1172, 2970
AcsI RAATTY 8 cut(s) 921, 1111, 1708, 1718, 2171, 2301, 2521, 2679
AcyI GRCGYC 1 cut(s) 150
AdeI CACNNNGTG 2 cut(s) 2030, 3018
AfaI GTAC 6 cut(s) 98, 503, 1105, 2536, 2961, 3109
AflII CTTAAG 1 cut(s) 1126
AflIII ACRYGT 1 cut(s) 736
AjnI CCWGG 4 cut(s) 272, 1455, 1503, 1669
AjuI GAANNNNNNNTTGG 6 cut(s) 248, 280, 1322, 1354, 1644, 1676
Alw21I GWGCWC 4 cut(s) 326, 567, 1536, 2640
Alw26I GTCTC 6 cut(s) 344, 520, 1483, 1605, 1784, 2655
Ama87I CYCGRG 1 cut(s) 1535
AoxI GGCC 7 cut(s) 426, 1172, 1308, 2071, 2265, 2970, 2994
ApeKI GCWGC 8 cut(s) 595, 835, 1316, 1848, 2207, 2329, 2579, 2720
ApoI RAATTY 8 cut(s) 921, 1111, 1708, 1718, 2171, 2301, 2521, 2679
Asp700I GAANNNNTTC 1 cut(s) 921
Asp718I GGTACC 1 cut(s) 96
AspA2I CCTAGG 1 cut(s) 1387
AspLEI GCGC 4 cut(s) 152, 158, 792, 794
AspS9I GGNCC 5 cut(s) 228, 1501, 2006, 2265, 2377
AsuHPI GGTGA 3 cut(s) 155, 1676, 2645
AsuII TTCGAA 1 cut(s) 2165
AvaI CYCGRG 1 cut(s) 1535
AvaII GGWCC 4 cut(s) 228, 1501, 2006, 2377
AvrII CCTAGG 1 cut(s) 1387
BalI TGGCCA 1 cut(s) 2972
BamHI GGATCC 1 cut(s) 1203
BanI GGYRCC 2 cut(s) 96, 149
BanII GRGCYC 3 cut(s) 326, 1536, 2640
BauI CACGAG 1 cut(s) 1977
BbsI GAAGAC 2 cut(s) 10, 251
Bbv12I GWGCWC 4 cut(s) 326, 567, 1536, 2640
BbvI GCAGC 8 cut(s) 607, 822, 1328, 1860, 2194, 2341, 2566, 2707
BccI CCATC 9 cut(s) 140, 213, 478, 929, 1079, 1193, 2017, 2843, 3023
BceAI ACGGC 2 cut(s) 475, 1159
BciT130I CCWGG 4 cut(s) 274, 1457, 1505, 1671
BciVI GTATCC 5 cut(s) 477, 616, 718, 1363, 1405
BcoDI GTCTC 6 cut(s) 344, 520, 1483, 1605, 1784, 2655
BfaI CTAG 6 cut(s) 333, 944, 1388, 1421, 1475, 1964
BfmI CTRYAG 3 cut(s) 1147, 2748, 2979
BfoI RGCGCY 1 cut(s) 153
BfrI CTTAAG 1 cut(s) 1126
BfuAI ACCTGC 1 cut(s) 1990
BfuI GTATCC 5 cut(s) 477, 616, 718, 1363, 1405
BglI GCCNNNNNGGC 1 cut(s) 1171
BglII AGATCT 2 cut(s) 889, 1417
BlnI CCTAGG 1 cut(s) 1387
Bme1390I CCNGG 4 cut(s) 274, 1457, 1505, 1671
Bme18I GGWCC 4 cut(s) 228, 1501, 2006, 2377
BmeT110I CYCGRG 1 cut(s) 1535
BmgT120I GGNCC 5 cut(s) 228, 1501, 2006, 2265, 2377
BmrFI CCNGG 4 cut(s) 274, 1457, 1505, 1671
BmsI GCATC 6 cut(s) 45, 52, 1131, 2219, 2803, 2826
BpiI GAAGAC 2 cut(s) 10, 251
BplI GAGNNNNNCTC 2 cut(s) 2768, 2800
BpmI CTGGAG 2 cut(s) 695, 1550
Bpu10I CCTNAGC 2 cut(s) 720, 2469
Bpu14I TTCGAA 1 cut(s) 2165
BpuEI CTTGAG 2 cut(s) 1283, 1587
BsaBI GATNNNNATC 3 cut(s) 440, 939, 1416
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 520
BsaJI CCNNGG 9 cut(s) 273, 457, 1169, 1175, 1387, 1455, 1456, 2109, 2598
BsaWI WCCGGW 1 cut(s) 2458
BsaXI ACNNNNNCTCC 2 cut(s) 3072, 3102
Bse1I ACTGG 1 cut(s) 217
Bse3DI GCAATG 1 cut(s) 2896
Bse8I GATNNNNATC 3 cut(s) 440, 939, 1416
BseBI CCWGG 4 cut(s) 274, 1457, 1505, 1671
BseDI CCNNGG 9 cut(s) 273, 457, 1169, 1175, 1387, 1455, 1456, 2109, 2598
BseGI GGATG 7 cut(s) 60, 390, 1576, 2210, 2698, 2854, 3034
BseJI GATNNNNATC 3 cut(s) 440, 939, 1416
BseMI GCAATG 1 cut(s) 2896
BseMII CTCAG 7 cut(s) 339, 734, 1349, 1650, 2229, 2313, 2575
BseNI ACTGG 1 cut(s) 217
BsePI GCGCGC 1 cut(s) 790
BseRI GAGGAG 2 cut(s) 1778, 2881
BseXI GCAGC 8 cut(s) 607, 822, 1328, 1860, 2194, 2341, 2566, 2707
BseYI CCCAGC 2 cut(s) 1621, 1851
BsgI GTGCAG 1 cut(s) 2781
Bsh1236I CGCG 3 cut(s) 156, 792, 2877
BshFI GGCC 7 cut(s) 428, 1174, 1310, 2073, 2267, 2972, 2996
BshNI GGYRCC 2 cut(s) 96, 149
BsiHKAI GWGCWC 4 cut(s) 326, 567, 1536, 2640
BsiHKCI CYCGRG 1 cut(s) 1535
BsiSI CCGG 2 cut(s) 2459, 2840
BslFI GGGAC 1 cut(s) 2019
BsmAI GTCTC 6 cut(s) 344, 520, 1483, 1605, 1784, 2655
BsmFI GGGAC 1 cut(s) 2019
BsmI GAATGC 2 cut(s) 2103, 3014
BsnI GGCC 7 cut(s) 428, 1174, 1310, 2073, 2267, 2972, 2996
Bso31I GGTCTC 1 cut(s) 520
BsoBI CYCGRG 1 cut(s) 1535
Bsp119I TTCGAA 1 cut(s) 2165
Bsp1286I GDGCHC 4 cut(s) 326, 567, 1536, 2640
Bsp19I CCATGG 1 cut(s) 2598
BspANI GGCC 7 cut(s) 428, 1174, 1310, 2073, 2267, 2972, 2996
BspCNI CTCAG 7 cut(s) 338, 733, 1348, 1649, 2228, 2314, 2574
BspFNI CGCG 3 cut(s) 156, 792, 2877
BspHI TCATGA 2 cut(s) 2076, 2550
BspMAI CTGCAG 2 cut(s) 1151, 2983
BspMI ACCTGC 1 cut(s) 1990
BspT104I TTCGAA 1 cut(s) 2165
BspT107I GGYRCC 2 cut(s) 96, 149
BspTI CTTAAG 1 cut(s) 1126
BspTNI GGTCTC 1 cut(s) 520
BsrDI GCAATG 1 cut(s) 2896
BsrI ACTGG 1 cut(s) 217
BssECI CCNNGG 9 cut(s) 273, 457, 1169, 1175, 1387, 1455, 1456, 2109, 2598
BssHII GCGCGC 1 cut(s) 790
BssNAI GTATAC 1 cut(s) 1630
BssNI GRCGYC 1 cut(s) 150
BssSI CACGAG 1 cut(s) 1977
BssT1I CCWWGG 3 cut(s) 1387, 2109, 2598
Bst1107I GTATAC 1 cut(s) 1630
Bst2BI CACGAG 1 cut(s) 1977
Bst2UI CCWGG 4 cut(s) 274, 1457, 1505, 1671
Bst4CI ACNGT 8 cut(s) 730, 1170, 1249, 1351, 1382, 1587, 1750, 2752
Bst6I CTCTTC 4 cut(s) 251, 335, 1872, 2750
BstACI GRCGYC 1 cut(s) 150
BstAFI CTTAAG 1 cut(s) 1126
BstAPI GCANNNNNTGC 2 cut(s) 1141, 2207
BstBI TTCGAA 1 cut(s) 2165
BstDSI CCRYGG 4 cut(s) 457, 1169, 1175, 2598
BstENI CCTNNNNNAGG 2 cut(s) 1129, 3102
BstF5I GGATG 7 cut(s) 60, 390, 1576, 2210, 2698, 2854, 3034
BstFNI CGCG 3 cut(s) 156, 792, 2877
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 4 cut(s) 152, 158, 792, 794
BstMAI GTCTC 6 cut(s) 344, 520, 1483, 1605, 1784, 2655
BstNI CCWGG 4 cut(s) 274, 1457, 1505, 1671
BstNSI RCATGY 4 cut(s) 740, 1063, 1405, 2960
BstSCI CCNGG 4 cut(s) 272, 1455, 1503, 1669
BstSFI CTRYAG 3 cut(s) 1147, 2748, 2979
BstUI CGCG 3 cut(s) 156, 792, 2877
BstV1I GCAGC 8 cut(s) 607, 822, 1328, 1860, 2194, 2341, 2566, 2707
BstV2I GAAGAC 2 cut(s) 10, 251
BstX2I RGATCY 9 cut(s) 177, 198, 306, 889, 1120, 1203, 1417, 1883, 2178
BstXI CCANNNNNNTGG 4 cut(s) 224, 1463, 1702, 2297
BstYI RGATCY 9 cut(s) 177, 198, 306, 889, 1120, 1203, 1417, 1883, 2178
BstZ17I GTATAC 1 cut(s) 1630
BsuI GTATCC 5 cut(s) 477, 616, 718, 1363, 1405
BsuRI GGCC 7 cut(s) 428, 1174, 1310, 2073, 2267, 2972, 2996
BtgI CCRYGG 4 cut(s) 457, 1169, 1175, 2598
BtsCI GGATG 7 cut(s) 60, 390, 1576, 2210, 2698, 2854, 3034
BtsI GCAGTG 3 cut(s) 1144, 1548, 2786
BtsIMutI CAGTG 9 cut(s) 333, 504, 1144, 1548, 1583, 1746, 1879, 2757, 2786
BveI ACCTGC 1 cut(s) 1990
CciI TCATGA 2 cut(s) 2076, 2550
CfoI GCGC 4 cut(s) 152, 158, 792, 794
Cfr13I GGNCC 5 cut(s) 228, 1501, 2006, 2265, 2377
Csp6I GTAC 6 cut(s) 97, 502, 1104, 2535, 2960, 3108
CspCI CAANNNNNGTGG 2 cut(s) 525, 560
CviQI GTAC 6 cut(s) 97, 502, 1104, 2535, 2960, 3108
DinI GGCGCC 1 cut(s) 151
DraI TTTAAA 1 cut(s) 33
DraIII CACNNNGTG 2 cut(s) 2030, 3018
EaeI YGGCCR 2 cut(s) 1172, 2970
Eam1104I CTCTTC 4 cut(s) 251, 335, 1872, 2750
EarI CTCTTC 4 cut(s) 251, 335, 1872, 2750
EciI GGCGGA 2 cut(s) 191, 963
Ecl136II GAGCTC 3 cut(s) 324, 1534, 2638
Eco130I CCWWGG 3 cut(s) 1387, 2109, 2598
Eco24I GRGCYC 3 cut(s) 326, 1536, 2640
Eco31I GGTCTC 1 cut(s) 520
Eco32I GATATC 2 cut(s) 2282, 2830
Eco47I GGWCC 4 cut(s) 228, 1501, 2006, 2377
Eco53kI GAGCTC 3 cut(s) 324, 1534, 2638
Eco88I CYCGRG 1 cut(s) 1535
EcoICRI GAGCTC 3 cut(s) 324, 1534, 2638
EcoNI CCTNNNNNAGG 2 cut(s) 1129, 3102
EcoRI GAATTC 4 cut(s) 921, 1708, 2301, 2521
EcoRII CCWGG 4 cut(s) 272, 1455, 1503, 1669
EcoRV GATATC 2 cut(s) 2282, 2830
EcoT14I CCWWGG 3 cut(s) 1387, 2109, 2598
EcoT38I GRGCYC 3 cut(s) 326, 1536, 2640
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
ErhI CCWWGG 3 cut(s) 1387, 2109, 2598
FalI AAGNNNNNCTT 8 cut(s) 248, 280, 339, 371, 502, 534, 800, 832
FaqI GGGAC 1 cut(s) 2019
FauI CCCGC 2 cut(s) 161, 2162
FauNDI CATATG 1 cut(s) 550
FblI GTMKAC 2 cut(s) 879, 1629
FokI GGATG 7 cut(s) 67, 397, 1583, 2197, 2705, 2861, 3041
FriOI GRGCYC 3 cut(s) 326, 1536, 2640
FspBI CTAG 6 cut(s) 333, 944, 1388, 1421, 1475, 1964
GlaI GCGC 4 cut(s) 151, 157, 791, 793
GsaI CCCAGC 2 cut(s) 1625, 1855
GsuI CTGGAG 2 cut(s) 695, 1550
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 7 cut(s) 428, 1174, 1310, 2073, 2267, 2972, 2996
HapII CCGG 2 cut(s) 2459, 2840
HhaI GCGC 4 cut(s) 152, 158, 792, 794
Hin1I GRCGYC 1 cut(s) 150
Hin6I GCGC 4 cut(s) 150, 156, 790, 792
HinP1I GCGC 4 cut(s) 150, 156, 790, 792
HindIII AAGCTT 1 cut(s) 2512
HpaII CCGG 2 cut(s) 2459, 2840
HphI GGTGA 3 cut(s) 155, 1676, 2645
Hpy166II GTNNAC 4 cut(s) 504, 880, 1630, 2707
Hpy8I GTNNAC 4 cut(s) 504, 880, 1630, 2707
Hpy99I CGWCG 1 cut(s) 1909
HpyAV CCTTC 5 cut(s) 760, 906, 1668, 1850, 2489
HpyCH4III ACNGT 8 cut(s) 730, 1170, 1249, 1351, 1382, 1587, 1750, 2752
HpyCH4IV ACGT 2 cut(s) 1785, 3082
HpySE526I ACGT 2 cut(s) 1785, 3082
Hsp92I GRCGYC 1 cut(s) 150
HspAI GCGC 4 cut(s) 150, 156, 790, 792
KasI GGCGCC 1 cut(s) 149
KpnI GGTACC 1 cut(s) 100
LmnI GCTCC 9 cut(s) 570, 676, 709, 829, 1048, 1531, 1618, 1765, 2643
Lsp1109I GCAGC 8 cut(s) 607, 822, 1328, 1860, 2194, 2341, 2566, 2707
LweI GCATC 6 cut(s) 45, 52, 1131, 2219, 2803, 2826
MaeI CTAG 6 cut(s) 333, 944, 1388, 1421, 1475, 1964
MaeII ACGT 2 cut(s) 1785, 3082
MaeIII GTNAC 6 cut(s) 966, 981, 1426, 1581, 1744, 2609
MfeI CAATTG 2 cut(s) 477, 1197
MflI RGATCY 9 cut(s) 177, 198, 306, 889, 1120, 1203, 1417, 1883, 2178
MhlI GDGCHC 4 cut(s) 326, 567, 1536, 2640
MlsI TGGCCA 1 cut(s) 2972
MluNI TGGCCA 1 cut(s) 2972
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 3 cut(s) 346, 974, 1769
MmeI TCCRAC 1 cut(s) 1933
Mox20I TGGCCA 1 cut(s) 2972
MroXI GAANNNNTTC 1 cut(s) 921
MscI TGGCCA 1 cut(s) 2972
MseI TTAA 9 cut(s) 32, 102, 402, 840, 1109, 1127, 2175, 2441, 2451
MslI CAYNNNNRTG 2 cut(s) 651, 1406
Msp20I TGGCCA 1 cut(s) 2972
MspA1I CMGCKG 5 cut(s) 1319, 1851, 2138, 2157, 2769
MspCI CTTAAG 1 cut(s) 1126
MspI CCGG 2 cut(s) 2459, 2840
MspR9I CCNGG 4 cut(s) 274, 1457, 1505, 1671
MunI CAATTG 2 cut(s) 477, 1197
Mva1269I GAATGC 2 cut(s) 2103, 3014
MvaI CCWGG 4 cut(s) 274, 1457, 1505, 1671
MvnI CGCG 3 cut(s) 156, 792, 2877
NarI GGCGCC 1 cut(s) 150
NcoI CCATGG 1 cut(s) 2598
NdeI CATATG 1 cut(s) 550
NmuCI GTSAC 3 cut(s) 966, 1581, 1744
NspI RCATGY 4 cut(s) 740, 1063, 1405, 2960
NspV TTCGAA 1 cut(s) 2165
PaeR7I CTCGAG 1 cut(s) 1535
PagI TCATGA 2 cut(s) 2076, 2550
PaqCI CACCTGC 1 cut(s) 1990
PasI CCCWGGG 1 cut(s) 1456
PauI GCGCGC 1 cut(s) 790
PciI ACATGT 1 cut(s) 736
PcsI WCGNNNNNNNCGW 1 cut(s) 1901
PctI GAATGC 2 cut(s) 2103, 3014
PdmI GAANNNNTTC 1 cut(s) 921
PfeI GAWTC 9 cut(s) 436, 800, 951, 1152, 1517, 1796, 1956, 2162, 2408
PflFI GACNNNGTC 1 cut(s) 641
PflMI CCANNNNNTGG 1 cut(s) 93
PfoI TCCNGGA 1 cut(s) 1669
PleI GAGTC 3 cut(s) 346, 973, 1769
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 3 cut(s) 346, 973, 1769
PscI ACATGT 1 cut(s) 736
PsiI TTATAA 1 cut(s) 1487
Psp124BI GAGCTC 3 cut(s) 326, 1536, 2640
Psp6I CCWGG 4 cut(s) 272, 1455, 1503, 1669
PspFI CCCAGC 2 cut(s) 1621, 1851
PspGI CCWGG 4 cut(s) 272, 1455, 1503, 1669
PspPI GGNCC 5 cut(s) 228, 1501, 2006, 2265, 2377
PspXI VCTCGAGB 1 cut(s) 1535
PstI CTGCAG 2 cut(s) 1151, 2983
PsuI RGATCY 9 cut(s) 177, 198, 306, 889, 1120, 1203, 1417, 1883, 2178
PsyI GACNNNGTC 1 cut(s) 641
PteI GCGCGC 1 cut(s) 790
PvuII CAGCTG 2 cut(s) 1319, 1851
RsaI GTAC 6 cut(s) 98, 503, 1105, 2536, 2961, 3109
RsaNI GTAC 6 cut(s) 97, 502, 1104, 2535, 2960, 3108
RseI CAYNNNNRTG 2 cut(s) 651, 1406
SacI GAGCTC 3 cut(s) 326, 1536, 2640
SaqAI TTAA 9 cut(s) 32, 102, 402, 840, 1109, 1127, 2175, 2441, 2451
Sau96I GGNCC 5 cut(s) 228, 1501, 2006, 2265, 2377
SchI GAGTC 3 cut(s) 346, 974, 1769
ScrFI CCNGG 4 cut(s) 274, 1457, 1505, 1671
SduI GDGCHC 4 cut(s) 326, 567, 1536, 2640
SfaNI GCATC 6 cut(s) 45, 52, 1131, 2219, 2803, 2826
SfcI CTRYAG 3 cut(s) 1147, 2748, 2979
SfoI GGCGCC 1 cut(s) 151
Sfr274I CTCGAG 1 cut(s) 1535
SfuI TTCGAA 1 cut(s) 2165
SinI GGWCC 4 cut(s) 228, 1501, 2006, 2377
SlaI CTCGAG 1 cut(s) 1535
SmiMI CAYNNNNRTG 2 cut(s) 651, 1406
SmlI CTYRAG 4 cut(s) 1126, 1298, 1535, 1602
SmoI CTYRAG 4 cut(s) 1126, 1298, 1535, 1602
SspDI GGCGCC 1 cut(s) 149
SspI AATATT 2 cut(s) 2905, 2944
SspMI CTAG 6 cut(s) 333, 944, 1388, 1421, 1475, 1964
SstI GAGCTC 3 cut(s) 326, 1536, 2640
StyD4I CCNGG 4 cut(s) 272, 1455, 1503, 1669
StyI CCWWGG 3 cut(s) 1387, 2109, 2598
TaaI ACNGT 8 cut(s) 730, 1170, 1249, 1351, 1382, 1587, 1750, 2752
TaiI ACGT 2 cut(s) 1788, 3085
TauI GCSGC 2 cut(s) 1096, 1848
TfiI GAWTC 9 cut(s) 436, 800, 951, 1152, 1517, 1796, 1956, 2162, 2408
Tru1I TTAA 9 cut(s) 32, 102, 402, 840, 1109, 1127, 2175, 2441, 2451
Tru9I TTAA 9 cut(s) 32, 102, 402, 840, 1109, 1127, 2175, 2441, 2451
TscAI CASTG 9 cut(s) 333, 511, 1151, 1548, 1590, 1753, 1879, 2757, 2793
TseFI GTSAC 3 cut(s) 966, 1581, 1744
TseI GCWGC 8 cut(s) 595, 835, 1316, 1848, 2207, 2329, 2579, 2720
Tsp45I GTSAC 3 cut(s) 966, 1581, 1744
TspGWI ACGGA 2 cut(s) 2035, 2641
TspRI CASTG 9 cut(s) 333, 511, 1151, 1548, 1590, 1753, 1879, 2757, 2793
Tth111I GACNNNGTC 1 cut(s) 641
Van91I CCANNNNNTGG 1 cut(s) 93
Vha464I CTTAAG 1 cut(s) 1126
VpaK11BI GGWCC 4 cut(s) 228, 1501, 2006, 2377
XagI CCTNNNNNAGG 2 cut(s) 1129, 3102
XapI RAATTY 8 cut(s) 921, 1111, 1708, 1718, 2171, 2301, 2521, 2679
XbaI TCTAGA 2 cut(s) 1420, 1963
XceI RCATGY 4 cut(s) 740, 1063, 1405, 2960
XcmI CCANNNNNNNNNTGG 1 cut(s) 1173
XhoI CTCGAG 1 cut(s) 1535
XmaJI CCTAGG 1 cut(s) 1387
XmiI GTMKAC 2 cut(s) 879, 1629
XmnI GAANNNNTTC 1 cut(s) 921
XspI CTAG 6 cut(s) 333, 944, 1388, 1421, 1475, 1964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.