MD16G1068400.v1.1

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014276
Physical Location & Seq
Forward (+)
4792508 .. 4810064
17557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1068400.v1.1.491

Sequence Viewer

Length: 3081 bp
ATGCTGTGGTGGATGAGTTTCTTCCCTGTATTTGGGTTCGGTTTGAAGGTGGGGCGTCTGCTTTGGATGCTATGTTGCTCGATTGTATCTGTAATTTCCATGAACTGGTACCTCACTGATGGGATTGTGACCGGCACCAAGCTAGGTTTGCTTGGTGAAGCTGCCAATATGTGCCTCAAATGGTGGAAGAAAATCCCACTGAACATCAGCAAGATCCGCCAACATTACTACCAGTACGGGCTGAAGAGAGTCCGAAAAACGTGGTCGAGGAAGCTTGTGTTTTCGTGGGTGGTTGGGTGGATAATTGTGAGCCTCTGGAACTTCCCTTTCATCATCTCACAGGCCAGTGAGAAGAGGAAGGAAACTCTTGCAAGGATGTGTGATGAGAGGGCGAGGATGTTGCAGGATCAATTTAATGTCAGCATGAACCATATTCAGGCCATGTCGATGCTGATCTCGACATTCCACGACGTAAAGTACCCTTCGGCCATTGATCAGAAAACTTTTGCGCGGTACATTGAAAGAACTGCTTTCGAGAGACCCCTCACAAGTGGCGTGGCATATGCTGTAAGGGTGCTCCACTCGGAAAAAGAAGAATTTGAGAAGCAGCAGGGCTGGAGTATCAAGAGAATGGATACCCTTGAACAAAACCAGGTTCATAAGAATGACTATGCCCCAGAAGTTCAGGAAGAATATGCTCCTGTTATCTTTGCACAGGATAACATTAGACATGTCATCTCTTTTGATATGCTGTCTGGGAAGGAAGATCGACAAAACGTGTTGCGTGCAAGAGCAGTGGGAAAGGGGGTTCTAACTGCTCCTTTTCGGCTGCTTAAAACAAACAGACTTGGAGTAATACTGACATTTGCTGTCTACAAAAGAAATCTGCCCTCAAATGCAACTCCAACCGAAAGAATTCAAGCGACTGATGGGTATCTCGGCGGACTATTTCATTTTGAGTCACTAGTGGAGAAGTCACTTGAACAACTCGCTAGCAAGCAAACCATCCTTGTGAACGTGTATGACACTACCAACCAGTCGCATCCAATCAGAATGTACGGTTCACAGGTTTCAGATGATGGATTGCAGCACATCAGCTCCTTAGGTTTTGGAGATCCCTTAAGGAACCATGAGATGCGGTGCAGATTCAAACATAAGCCACCACGGCCGTGGTTAGCCATTGCAACTTCTAGTAGTTTGATCCTCGAGATTGCATTGCATGTTGGCCACATTTTGATTGCAGCTGCAAGTACGATTGCAAAAGTGGAGGATGATTTCTGTATGATGATGGAGCTCAAGAAACAGGCCAAGGCAGTTTATGCTGCCACATCTAAGTTTCTTTCAAATGTTTTACACTGGATCAAAAGCCCAATTAATGGTGTCTTAGGAAAGTTTAACATGCTATTGGAGACAAACCTGGTTGTTACTCAACTAGATTATTTCCGAACTACACATAGACGTAAAAGATGTGTAGTCTCACTTAAAAATGAGGTCTTGGACCATACAAATATTGAAACTGGCAAGCTGGAGTTTGAGGCAGTGCGTTTTGATCTGCGAGCTATTTTGGATGATGTTCTGTCCTTCTTTTTGAATAAGTATCAAGAAGAAGTAGAGCAGTGGGCGGTATACATCTCAGATCAGGTTCCTAATATGCTTATTGGTGATCCCGGAAGGTTTCAGCAGATTATCACCAATCTCATGTGGAATTCAATCGAATTCACAGAGAAGGGACACATCTTTGTCACGGTTCATCTTGTCGAAGAGCTGATTGGCTCAACAGATGTTGAGACTGAATCATCATCGAAGAACACTTTGAGTCATTTCCCCGTTGCAGATAGACGCCGCAGTTGGGGCGGATTTAGGTGTTTCAGTCAAGATGCGAGCCGTTTTTCATCGTCACCTGACCTCATCCATATTATTGTATCTGTTGAGGACACAGGATTGGGGATTCCCCTAGAAGCCCAATATCTTCTTTTAACTCCTTTTATGGATTTGGGACATATAGATAGAGGAATTGAAATAAGGATAAGGATAAGCCAGTGTTTGGTCAGCCTCATGAAAGGGGAAATCGGCTTTGTTAGCATTCCGAAGATTGGTTCCACATTTACATTTACTGCTGTTTTCACCAATGCCAGCTCCAGTTCAAATGAGTTAACGATCCAGCAAATCAGCAGCCGGTCAAATGCTGCATCCTCAGAATTTAATGGCATGACGGCATTAGTTGTGGACCAAAGACCTGTTAGGGCCAAGATGTCAAGATATCATATTCAACGGCTTGGAATTCGTGTTGAAGTAGTTTCTGATTTGTATGAGGGTTTATCTAGCATAAGCTGTGGGAGTACAACTATCAATATGGTCCTTGTCGAACAGGAAGTCTGGGATAAAGATTCGGGAACTTCAGCTCTCTTTGTCCGTAATTTAAGGAAAATTGTTGGACAGGTTCCTCCGAACCTGTTTATTCTTACCAATTCTATTAGCTCATGCAGAATAAGCTCTGCAACTTCAGGTGTCCCTACACCAACTGTCATCAAGAAGCCTCTCATGGCAAGCACGCTCGCTGCCTCACTGCAAAGGGCCATGGGAGCTGGTAAGGGAAATCTCCGGAACGGGGAGCTCCCAAGTTTGTCTCTCCGCGATCTTCTGCTCGGAAGAAGAATTCTTATTATAGATGATGATAATGTGAATCTCAGAGTAGCTGCCGCTGCTTTGGAAAAGCATGGGGCATGGGTGGTCTGTGCAGACAGTGGTGAAAGGGCAATCTCACTGCTTATGCCGCCCCACCATTTTGATGCCTGCTTCATGGATATCCAGATGCCAGAGATGGATGGGTTTGAATCTACAAGGAGAATACGTAACATGGAACGCAATATCAGCAGCAGTATTCACCATGGCGACGTTTCTGTGGAGGATTATGAAAATATTCGAGCATGGCATGTACCCATTCTGGCCATGACTGCTGATGTAATTCAGTCTACCCACGAGGAATGCACAAAGTGCGGAATGGATGGATGTGTTTCAAAACCCTTCGAAGCTGAACAGCTTTATCGCGCAGTTTCCCCTTTTTTCCTATCTACAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009743 GO:0009744 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0009991 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010150 GO:0010271 GO:0010959 GO:0012505 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034285 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042175 GO:0042221 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048366 GO:0048367 GO:0048509 GO:0048519 GO:0048580 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071396 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0090693 GO:0097305 GO:0097306 GO:0098542 GO:0098827 GO:0099402 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1027

Amino Acids

115.92

Weight (kDa)

8.51

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 163 - 353 5.7e-36 CHASE domain
Receiver_CRE1 PF24896 731 - 861 1.7e-38 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 886 - 956 3.8e-14 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27320
fragaria_vesca FvH4_4g23590 FvH4_4g23590 FvH4_4g23590 FvH4_4g23590
malus_domestica MD13G1120900.v1.1 MD16G1068400.v1.1 MD16G1120900.v1.1
prunus_persica Prupe.1G224300_v2.0.a1
pyrus_communis pycom13g10520
rosa_chinensis RchiOBHm_Chr4g0430011
rosa_laevigata RLG00000007014
rosa_roxburghii Rroxscaffold_5G00371820
rosa_rugosa Rorug04G0239800
rosa_samantha Rh4BG302900 Rh4CG318400
rosa_wichuraiana Rw4G025680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 108
AccB1I GGYRCC 2 cut(s) 108, 134
AccB7I CCANNNNNTGG 3 cut(s) 105, 1376, 2044
AccI GTMKAC 3 cut(s) 873, 1626, 2972
AccII CGCG 3 cut(s) 511, 2634, 3048
AccIII TCCGGA 1 cut(s) 2601
AclWI GGATC 7 cut(s) 208, 414, 1109, 1195, 1367, 1658, 2152
AcoI YGGCCR 4 cut(s) 486, 1166, 1225, 2946
AcsI RAATTY 7 cut(s) 596, 915, 1705, 1715, 2198, 2280, 2655
AcuI CTGAAG 3 cut(s) 263, 2382, 2487
AcyI GRCGYC 2 cut(s) 55, 1840
AdeI CACNNNGTG 1 cut(s) 2994
AfaI GTAC 8 cut(s) 110, 236, 479, 515, 1058, 1252, 2341, 2937
AfiI CCNNNNNNNGG 8 cut(s) 32, 105, 436, 1376, 1849, 2044, 2093, 2608
AflII CTTAAG 1 cut(s) 1120
AflIII ACRYGT 3 cut(s) 730, 777, 1017
AhlI ACTAGT 1 cut(s) 964
AjnI CCWGG 2 cut(s) 651, 1416
AjuI GAANNNNNNNTTGG 2 cut(s) 1752, 1784
AleI CACNNNNGTG 1 cut(s) 1168
AloI GAACNNNNNNTCC 2 cut(s) 792, 824
Alw21I GWGCWC 3 cut(s) 579, 1296, 2616
Alw26I GTCTC 5 cut(s) 532, 1403, 1480, 1781, 2631
AlwI GGATC 7 cut(s) 208, 414, 1109, 1195, 1367, 1658, 2152
Ama87I CYCGRG 1 cut(s) 1205
Aor13HI TCCGGA 1 cut(s) 2601
AoxI GGCC 9 cut(s) 342, 438, 486, 1166, 1225, 1305, 2244, 2574, 2946
ApoI RAATTY 7 cut(s) 596, 915, 1705, 1715, 2198, 2280, 2655
ArsI GACNNNNNNTTYG 2 cut(s) 2358, 2390
AseI ATTAAT 1 cut(s) 1374
Asp700I GAANNNNTTC 2 cut(s) 915, 2919
Asp718I GGTACC 1 cut(s) 108
AspLEI GCGC 2 cut(s) 511, 3050
AspS9I GGNCC 5 cut(s) 1498, 2227, 2244, 2356, 2574
AsuC2I CCSGG 1 cut(s) 1668
AsuHPI GGTGA 7 cut(s) 167, 1673, 1681, 1890, 2116, 2759, 2876
AsuII TTCGAA 1 cut(s) 3027
AsuNHI GCTAGC 1 cut(s) 992
AvaI CYCGRG 1 cut(s) 1205
AvaII GGWCC 3 cut(s) 1498, 2227, 2356
AxyI CCTNAGG 1 cut(s) 1102
BalI TGGCCA 2 cut(s) 1227, 2948
BanI GGYRCC 2 cut(s) 108, 134
BanII GRGCYC 2 cut(s) 1296, 2616
BauI CACGAG 1 cut(s) 2978
Bbv12I GWGCWC 3 cut(s) 579, 1296, 2616
BccI CCATC 8 cut(s) 113, 923, 1013, 1073, 1282, 2815, 2819, 2999
BceAI ACGGC 5 cut(s) 1153, 1181, 1869, 2229, 2288
BcgI CGANNNNNNTGC 2 cut(s) 382, 416
BciT130I CCWGG 2 cut(s) 653, 1418
BciVI GTATCC 1 cut(s) 628
BclI TGATCA 1 cut(s) 493
BcnI CCSGG 1 cut(s) 1668
BcoDI GTCTC 5 cut(s) 532, 1403, 1480, 1781, 2631
BcuI ACTAGT 1 cut(s) 964
BfaI CTAG 7 cut(s) 143, 965, 993, 1191, 1433, 1955, 2322
BfrI CTTAAG 1 cut(s) 1120
BfuI GTATCC 1 cut(s) 628
BglI GCCNNNNNGGC 1 cut(s) 1165
Bme1390I CCNGG 3 cut(s) 653, 1418, 1668
Bme18I GGWCC 3 cut(s) 1498, 2227, 2356
BmeT110I CYCGRG 1 cut(s) 1205
BmgT120I GGNCC 5 cut(s) 1498, 2227, 2244, 2356, 2574
BmiI GGNNCC 6 cut(s) 110, 136, 1127, 1644, 2098, 2442
BmrFI CCNGG 3 cut(s) 653, 1418, 1668
BmsI GCATC 8 cut(s) 57, 438, 1051, 1125, 1867, 2198, 2779, 2802
BmtI GCTAGC 1 cut(s) 996
BpmI CTGGAG 3 cut(s) 637, 1547, 2122
Bpu14I TTCGAA 1 cut(s) 3027
BpuEI CTTGAG 1 cut(s) 1280
BpuMI CCSGG 1 cut(s) 1668
BsaAI YACGTR 1 cut(s) 2852
BsaBI GATNNNNATC 2 cut(s) 452, 933
BsaHI GRCGYC 2 cut(s) 55, 1840
BsaI GGTCTC 1 cut(s) 532
BsaJI CCNNGG 5 cut(s) 1163, 1169, 1308, 2577, 2887
BsaWI WCCGGW 1 cut(s) 2601
BsaXI ACNNNNNCTCC 4 cut(s) 1082, 1112, 2836, 2866
Bsc4I CCNNNNNNNGG 8 cut(s) 32, 105, 436, 1376, 1849, 2044, 2093, 2608
Bse118I RCCGGY 2 cut(s) 131, 2175
Bse1I ACTGG 8 cut(s) 110, 232, 345, 1036, 1361, 1522, 2038, 2139
Bse21I CCTNAGG 1 cut(s) 1102
Bse3DI GCAATG 2 cut(s) 1179, 1214
Bse8I GATNNNNATC 2 cut(s) 452, 933
BseAI TCCGGA 1 cut(s) 2601
BseBI CCWGG 2 cut(s) 653, 1418
BseDI CCNNGG 5 cut(s) 1163, 1169, 1308, 2577, 2887
BseJI GATNNNNATC 2 cut(s) 452, 933
BseLI CCNNNNNNNGG 8 cut(s) 32, 105, 436, 1376, 1849, 2044, 2093, 2608
BseMI GCAATG 2 cut(s) 1179, 1214
BseMII CTCAG 3 cut(s) 1647, 2208, 2701
BseNI ACTGG 8 cut(s) 110, 232, 345, 1036, 1361, 1522, 2038, 2139
BseX3I CGGCCG 1 cut(s) 1166
BsgI GTGCAG 2 cut(s) 1162, 2757
Bsh1236I CGCG 3 cut(s) 511, 2634, 3048
Bsh1285I CGRYCG 1 cut(s) 1169
BshFI GGCC 9 cut(s) 344, 440, 488, 1168, 1227, 1307, 2246, 2576, 2948
BshNI GGYRCC 2 cut(s) 108, 134
BsiEI CGRYCG 1 cut(s) 1169
BsiHKAI GWGCWC 3 cut(s) 579, 1296, 2616
BsiHKCI CYCGRG 1 cut(s) 1205
BsiSI CCGG 4 cut(s) 132, 1668, 2176, 2602
BslFI GGGAC 3 cut(s) 1743, 2010, 2495
BslI CCNNNNNNNGG 8 cut(s) 32, 105, 436, 1376, 1849, 2044, 2093, 2608
BsmAI GTCTC 5 cut(s) 532, 1403, 1480, 1781, 2631
BsmFI GGGAC 3 cut(s) 1743, 2010, 2495
BsmI GAATGC 2 cut(s) 2082, 2990
BsnI GGCC 9 cut(s) 344, 440, 488, 1168, 1227, 1307, 2246, 2576, 2948
Bso31I GGTCTC 1 cut(s) 532
BsoBI CYCGRG 1 cut(s) 1205
Bsp119I TTCGAA 1 cut(s) 3027
Bsp1286I GDGCHC 3 cut(s) 579, 1296, 2616
Bsp13I TCCGGA 1 cut(s) 2601
Bsp19I CCATGG 2 cut(s) 2577, 2887
BspANI GGCC 9 cut(s) 344, 440, 488, 1168, 1227, 1307, 2246, 2576, 2948
BspCNI CTCAG 3 cut(s) 1646, 2207, 2700
BspEI TCCGGA 1 cut(s) 2601
BspFNI CGCG 3 cut(s) 511, 2634, 3048
BspHI TCATGA 1 cut(s) 2055
BspLI GGNNCC 6 cut(s) 110, 136, 1127, 1644, 2098, 2442
BspOI GCTAGC 1 cut(s) 996
BspPI GGATC 7 cut(s) 208, 414, 1109, 1195, 1367, 1658, 2152
BspQI GCTCTTC 1 cut(s) 1755
BspT104I TTCGAA 1 cut(s) 3027
BspT107I GGYRCC 2 cut(s) 108, 134
BspTI CTTAAG 1 cut(s) 1120
BspTNI GGTCTC 1 cut(s) 532
BsrDI GCAATG 2 cut(s) 1179, 1214
BsrFI RCCGGY 2 cut(s) 131, 2175
BsrI ACTGG 8 cut(s) 110, 232, 345, 1036, 1361, 1522, 2038, 2139
BssAI RCCGGY 2 cut(s) 131, 2175
BssECI CCNNGG 5 cut(s) 1163, 1169, 1308, 2577, 2887
BssNAI GTATAC 1 cut(s) 1627
BssNI GRCGYC 2 cut(s) 55, 1840
BssSI CACGAG 1 cut(s) 2978
BssT1I CCWWGG 3 cut(s) 1308, 2577, 2887
Bst1107I GTATAC 1 cut(s) 1627
Bst2BI CACGAG 1 cut(s) 2978
Bst2UI CCWGG 2 cut(s) 653, 1418
Bst4CI ACNGT 4 cut(s) 1061, 1747, 2524, 2744
Bst6I CTCTTC 3 cut(s) 239, 347, 1755
BstACI GRCGYC 2 cut(s) 55, 1840
BstAFI CTTAAG 1 cut(s) 1120
BstAPI GCANNNNNTGC 2 cut(s) 1319, 2994
BstBAI YACGTR 1 cut(s) 2852
BstBI TTCGAA 1 cut(s) 3027
BstDEI CTNAG 6 cut(s) 1102, 1332, 1384, 1633, 2194, 2687
BstDSI CCRYGG 4 cut(s) 1163, 1169, 2577, 2887
BstFNI CGCG 3 cut(s) 511, 2634, 3048
BstHHI GCGC 2 cut(s) 511, 3050
BstMAI GTCTC 5 cut(s) 532, 1403, 1480, 1781, 2631
BstMCI CGRYCG 1 cut(s) 1169
BstNI CCWGG 2 cut(s) 653, 1418
BstNSI RCATGY 4 cut(s) 734, 1223, 1402, 2936
BstSCI CCNGG 3 cut(s) 651, 1416, 1666
BstSNI TACGTA 1 cut(s) 2852
BstUI CGCG 3 cut(s) 511, 2634, 3048
BstX2I RGATCY 2 cut(s) 213, 1114
BstXI CCANNNNNNTGG 1 cut(s) 1170
BstYI RGATCY 2 cut(s) 213, 1114
BstZ17I GTATAC 1 cut(s) 1627
BstZI CGGCCG 1 cut(s) 1166
Bsu36I CCTNAGG 1 cut(s) 1102
BsuI GTATCC 1 cut(s) 628
BsuRI GGCC 9 cut(s) 344, 440, 488, 1168, 1227, 1307, 2246, 2576, 2948
BtgI CCRYGG 4 cut(s) 1163, 1169, 2577, 2887
BtsI GCAGTG 5 cut(s) 801, 1545, 1622, 2564, 2762
CciI TCATGA 1 cut(s) 2055
CfoI GCGC 2 cut(s) 511, 3050
Cfr10I RCCGGY 2 cut(s) 131, 2175
Cfr13I GGNCC 5 cut(s) 1498, 2227, 2244, 2356, 2574
CseI GACGC 2 cut(s) 44, 1848
CsiI ACCWGGT 2 cut(s) 651, 1416
Csp6I GTAC 8 cut(s) 109, 235, 478, 514, 1057, 1251, 2340, 2936
CspCI CAANNNNNGTGG 4 cut(s) 537, 572, 777, 812
CviQI GTAC 8 cut(s) 109, 235, 478, 514, 1057, 1251, 2340, 2936
DdeI CTNAG 6 cut(s) 1102, 1332, 1384, 1633, 2194, 2687
DraIII CACNNNGTG 1 cut(s) 2994
EaeI YGGCCR 4 cut(s) 486, 1166, 1225, 2946
EagI CGGCCG 1 cut(s) 1166
Eam1104I CTCTTC 3 cut(s) 239, 347, 1755
EarI CTCTTC 3 cut(s) 239, 347, 1755
EciI GGCGGA 3 cut(s) 206, 957, 1869
Ecl136II GAGCTC 2 cut(s) 1294, 2614
EclXI CGGCCG 1 cut(s) 1166
Eco105I TACGTA 1 cut(s) 2852
Eco130I CCWWGG 3 cut(s) 1308, 2577, 2887
Eco24I GRGCYC 2 cut(s) 1296, 2616
Eco31I GGTCTC 1 cut(s) 532
Eco32I GATATC 2 cut(s) 2261, 2806
Eco47I GGWCC 3 cut(s) 1498, 2227, 2356
Eco52I CGGCCG 1 cut(s) 1166
Eco53kI GAGCTC 2 cut(s) 1294, 2614
Eco57I CTGAAG 3 cut(s) 263, 2382, 2487
Eco81I CCTNAGG 1 cut(s) 1102
Eco88I CYCGRG 1 cut(s) 1205
EcoICRI GAGCTC 2 cut(s) 1294, 2614
EcoRI GAATTC 5 cut(s) 915, 1705, 1715, 2280, 2655
EcoRII CCWGG 2 cut(s) 651, 1416
EcoRV GATATC 2 cut(s) 2261, 2806
EcoT14I CCWWGG 3 cut(s) 1308, 2577, 2887
EcoT38I GRGCYC 2 cut(s) 1296, 2616
ErhI CCWWGG 3 cut(s) 1308, 2577, 2887
FalI AAGNNNNNCTT 2 cut(s) 514, 546
FaqI GGGAC 3 cut(s) 1743, 2010, 2495
FauNDI CATATG 1 cut(s) 562
FbaI TGATCA 1 cut(s) 493
FblI GTMKAC 3 cut(s) 873, 1626, 2972
FriOI GRGCYC 2 cut(s) 1296, 2616
FspBI CTAG 7 cut(s) 143, 965, 993, 1191, 1433, 1955, 2322
GlaI GCGC 2 cut(s) 510, 3049
GsuI CTGGAG 3 cut(s) 637, 1547, 2122
HaeIII GGCC 9 cut(s) 344, 440, 488, 1168, 1227, 1307, 2246, 2576, 2948
HapII CCGG 4 cut(s) 132, 1668, 2176, 2602
HgaI GACGC 2 cut(s) 44, 1848
HhaI GCGC 2 cut(s) 511, 3050
Hin1I GRCGYC 2 cut(s) 55, 1840
Hin6I GCGC 2 cut(s) 509, 3048
HinP1I GCGC 2 cut(s) 509, 3048
HincII GTYRAC 1 cut(s) 2154
HindII GTYRAC 1 cut(s) 2154
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 9 cut(s) 249, 959, 1146, 1793, 1816, 1948, 2387, 2683, 2834
HpaI GTTAAC 1 cut(s) 2154
HpaII CCGG 4 cut(s) 132, 1668, 2176, 2602
HphI GGTGA 7 cut(s) 167, 1673, 1681, 1890, 2116, 2759, 2876
Hpy166II GTNNAC 7 cut(s) 874, 1015, 1064, 1627, 2154, 2227, 2973
Hpy8I GTNNAC 7 cut(s) 874, 1015, 1064, 1627, 2154, 2227, 2973
Hpy99I CGWCG 2 cut(s) 473, 2897
HpyAV CCTTC 8 cut(s) 40, 352, 492, 754, 1591, 1665, 1720, 3034
HpyCH4III ACNGT 4 cut(s) 1061, 1747, 2524, 2744
HpyCH4IV ACGT 7 cut(s) 260, 471, 777, 1017, 1459, 2851, 2895
HpyF3I CTNAG 6 cut(s) 1102, 1332, 1384, 1633, 2194, 2687
HpySE526I ACGT 7 cut(s) 260, 471, 777, 1017, 1459, 2851, 2895
Hsp92I GRCGYC 2 cut(s) 55, 1840
HspAI GCGC 2 cut(s) 509, 3048
Kpn2I TCCGGA 1 cut(s) 2601
KpnI GGTACC 1 cut(s) 112
Ksp22I TGATCA 1 cut(s) 493
KspAI GTTAAC 1 cut(s) 2154
LguI GCTCTTC 1 cut(s) 1755
LmnI GCTCC 9 cut(s) 582, 703, 823, 1103, 1291, 2141, 2582, 2611, 2619
LweI GCATC 8 cut(s) 57, 438, 1051, 1125, 1867, 2198, 2779, 2802
MabI ACCWGGT 2 cut(s) 651, 1416
MaeI CTAG 7 cut(s) 143, 965, 993, 1191, 1433, 1955, 2322
MaeII ACGT 7 cut(s) 260, 471, 777, 1017, 1459, 2851, 2895
MaeIII GTNAC 7 cut(s) 127, 960, 975, 1423, 1741, 1896, 2852
MflI RGATCY 2 cut(s) 213, 1114
MhlI GDGCHC 3 cut(s) 579, 1296, 2616
MlsI TGGCCA 2 cut(s) 1227, 2948
MluNI TGGCCA 2 cut(s) 1227, 2948
MlyI GAGTC 3 cut(s) 258, 968, 1825
MmeI TCCRAC 2 cut(s) 929, 2413
Mox20I TGGCCA 2 cut(s) 1227, 2948
MroI TCCGGA 1 cut(s) 2601
MroXI GAANNNNTTC 2 cut(s) 915, 2919
MscI TGGCCA 2 cut(s) 1227, 2948
MslI CAYNNNNRTG 5 cut(s) 446, 663, 1010, 1168, 2787
Msp20I TGGCCA 2 cut(s) 1227, 2948
MspA1I CMGCKG 2 cut(s) 1244, 2702
MspCI CTTAAG 1 cut(s) 1120
MspI CCGG 4 cut(s) 132, 1668, 2176, 2602
MspR9I CCNGG 3 cut(s) 653, 1418, 1668
Mva1269I GAATGC 2 cut(s) 2082, 2990
MvaI CCWGG 2 cut(s) 653, 1418
MvnI CGCG 3 cut(s) 511, 2634, 3048
NciI CCSGG 1 cut(s) 1668
NcoI CCATGG 2 cut(s) 2577, 2887
NdeI CATATG 1 cut(s) 562
NheI GCTAGC 1 cut(s) 992
NlaIV GGNNCC 6 cut(s) 110, 136, 1127, 1644, 2098, 2442
NmeAIII GCCGAG 1 cut(s) 918
NmuCI GTSAC 5 cut(s) 127, 960, 975, 1741, 1896
NspI RCATGY 4 cut(s) 734, 1223, 1402, 2936
NspV TTCGAA 1 cut(s) 3027
OliI CACNNNNGTG 1 cut(s) 1168
PaeR7I CTCGAG 1 cut(s) 1205
PagI TCATGA 1 cut(s) 2055
PciI ACATGT 1 cut(s) 730
PciSI GCTCTTC 1 cut(s) 1755
PctI GAATGC 2 cut(s) 2082, 2990
PdmI GAANNNNTTC 2 cut(s) 915, 2919
PfeI GAWTC 6 cut(s) 1146, 1793, 1948, 2387, 2683, 2834
PflMI CCANNNNNTGG 3 cut(s) 105, 1376, 2044
PfoI TCCNGGA 1 cut(s) 1666
PleI GAGTC 3 cut(s) 257, 967, 1824
PpsI GAGTC 3 cut(s) 257, 967, 1824
Ppu21I YACGTR 1 cut(s) 2852
PscI ACATGT 1 cut(s) 730
PshBI ATTAAT 1 cut(s) 1374
Psp124BI GAGCTC 2 cut(s) 1296, 2616
Psp6I CCWGG 2 cut(s) 651, 1416
PspGI CCWGG 2 cut(s) 651, 1416
PspN4I GGNNCC 6 cut(s) 110, 136, 1127, 1644, 2098, 2442
PspPI GGNCC 5 cut(s) 1498, 2227, 2244, 2356, 2574
PsuI RGATCY 2 cut(s) 213, 1114
PvuII CAGCTG 1 cut(s) 1244
RsaI GTAC 8 cut(s) 110, 236, 479, 515, 1058, 1252, 2341, 2937
RsaNI GTAC 8 cut(s) 109, 235, 478, 514, 1057, 1251, 2340, 2936
RseI CAYNNNNRTG 5 cut(s) 446, 663, 1010, 1168, 2787
SacI GAGCTC 2 cut(s) 1296, 2616
SapI GCTCTTC 1 cut(s) 1755
Sau96I GGNCC 5 cut(s) 1498, 2227, 2244, 2356, 2574
SchI GAGTC 3 cut(s) 258, 968, 1825
ScrFI CCNGG 3 cut(s) 653, 1418, 1668
SduI GDGCHC 3 cut(s) 579, 1296, 2616
SexAI ACCWGGT 2 cut(s) 651, 1416
SfaNI GCATC 8 cut(s) 57, 438, 1051, 1125, 1867, 2198, 2779, 2802
Sfr274I CTCGAG 1 cut(s) 1205
SfuI TTCGAA 1 cut(s) 3027
SinI GGWCC 3 cut(s) 1498, 2227, 2356
SlaI CTCGAG 1 cut(s) 1205
SmiMI CAYNNNNRTG 5 cut(s) 446, 663, 1010, 1168, 2787
SmlI CTYRAG 3 cut(s) 1120, 1205, 1295
SmoI CTYRAG 3 cut(s) 1120, 1205, 1295
SnaBI TACGTA 1 cut(s) 2852
SpeI ACTAGT 1 cut(s) 964
SspI AATATT 2 cut(s) 1510, 2920
SspMI CTAG 7 cut(s) 143, 965, 993, 1191, 1433, 1955, 2322
SstI GAGCTC 2 cut(s) 1296, 2616
StyD4I CCNGG 3 cut(s) 651, 1416, 1666
StyI CCWWGG 3 cut(s) 1308, 2577, 2887
TaaI ACNGT 4 cut(s) 1061, 1747, 2524, 2744
TaiI ACGT 7 cut(s) 263, 474, 780, 1020, 1462, 2854, 2898
TatI WGTACW 1 cut(s) 2339
TauI GCSGC 3 cut(s) 1845, 2702, 2776
TfiI GAWTC 6 cut(s) 1146, 1793, 1948, 2387, 2683, 2834
TseFI GTSAC 5 cut(s) 127, 960, 975, 1741, 1896
Tsp45I GTSAC 5 cut(s) 127, 960, 975, 1741, 1896
TspGWI ACGGA 1 cut(s) 2402
Van91I CCANNNNNTGG 3 cut(s) 105, 1376, 2044
Vha464I CTTAAG 1 cut(s) 1120
VpaK11BI GGWCC 3 cut(s) 1498, 2227, 2356
VspI ATTAAT 1 cut(s) 1374
XapI RAATTY 7 cut(s) 596, 915, 1705, 1715, 2198, 2280, 2655
XceI RCATGY 4 cut(s) 734, 1223, 1402, 2936
XcmI CCANNNNNNNNNTGG 1 cut(s) 1167
XhoI CTCGAG 1 cut(s) 1205
XmiI GTMKAC 3 cut(s) 873, 1626, 2972
XmnI GAANNNNTTC 2 cut(s) 915, 2919
XspI CTAG 7 cut(s) 143, 965, 993, 1191, 1433, 1955, 2322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.