MD13G1120900.v1.1

Histidine kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014276
Physical Location & Seq
Forward (+)
8883411 .. 8889878
6468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1120900.v1.1.491

Sequence Viewer

Length: 3120 bp
ATGAGTGTTTTCCATGTATTTGGGTTCGGTTTAAAGGTGGGGCATCTGCTTTGGATGCTATGTTGCTGGATTGTATCTGTAATTTCCATGAACTGGTACCTCACTGGTGGGATTGTGACAGACACCAAGGCAGGATTGCTTGGTGAAGCTGCCAATATGTGCCTCAAATGGTGGGAGAAAATCCCAATGAACATCTGCAAGATCCGCTACCATTACTACCAGTATATCGGGTCGAAGAGAGTCCGAAAAAGGTGGTGGAAGGGGCTTTTGTTTACGTGGGTGGTTGGATGGACAATTGGGAGCCTCTGGATCTTGTGGTACATGAGCTCACAGGCTAGTGAGAAGAGGAAGGAGACTCTTGCAAGTATGTGTGATGAGAGGGCTAGGATGTTGCAGGATCAGTTTAATGTCAGCATGAACCATATTCAGGCCATGTCGATACTGATCTCGACATTCCACCACGGGAAGTACCCGTCTGCCATTGATCAGAAAACTTTTGCGCGGTACACTGAAAGAACTGCTTTTGAGAGACCCCTCACAAGTGGCGTGGCATATGCTGCAAGGGTGCTCCACTTGGAAAAGGAACAATTTGAGAAGCAGCAGGGCTGGACTATAAAGAGAATGGATACCCTCGAACAAAACCAGGTTCATAAGAATGACTATGCCCCAGAAGCCTTGGAACCATCCCCAGTTCAGGAAGAATATGCTCCTGTTATCTTTGCACAGGATACCGTTAGACACATCATCTCTTACGATATGCTGACCGGGAAGGAAGATCGACAAAACGTGTTGCGTGCTAGAGAATCAGGAAAGGGGGTTCTAACTGCTCCTTTTCGGTTGCTTAAAACAAACCGCCTTGGAGTAATACTGACATTTGCTGTCTACAAAAGAGATCTGCCCTCAAATGCAACTCCAAATGAAAGAATTCAAGCAACTGACGGGTATCTCGGTGGAATCTTTCATATTGAGTCACTTGTGGAGAAGTTACTTCAACAACTTGCTAGCAAGCAAACCATCCTTGTGAACGTGTATGACACTACCAATCAGTCGCATCCAATCAGCATGTATGGTTCAAATGTTTCAGATGATGGATTGCAGCACATAAGCTCCTTAAGTTTTGGAGATCCCTTAAGGAACCATGAGATGCGGTGCAGGTTCAAACATAAGCCACCATGGCCGTGGTTAGCCATTACAACTTCTTTTGGAATCCTCGTGATTGCATTTCTTGTTGGATACATTTTCCATGCAACTGTAAATAGGATTGCCAAAGTGGAGGATGATTTCCGTAAGATGATGGAGCTCAAGAAACAGGCTGAGGCAGCTGATGTTGCCAAATCTCAGTTTCTTGCAACTGTTTCGCACGAGATCAGAACCCCGATGAATGGTGTCCTAGGAATGTTACATATGCTAATGGACACAAATCTGGATGTTACTCAACTAGATTATGTCCGAACTGCCCAGGGAAGTGGAAAAGCTCTTGTCTCACTTATAAATGAGGTGTTGGACCAGGCAAAGATTGACTCTGGTAAGCTGGAGCTTGAGGCGTTGCGTTTTGATCTGCGAGCTATTCTGGATGATGTTTTGTCACTCTTTTCAGGGAAGTCTCAAGAAAAAGGAGTAGAGTTGGCAGTATACATCTCAGATCAGGTTCCTGATATGCTTATTGGTGATCCCGGAAGGTTTCGGCAGATTATCACCAATCTCATTGGGAATTCAATCAAATTCACAGAGAAAGGACACATCTTTGTCACGGTTCATCTTGTCGAGGAGCTGATTGGCTCGATAGATGTTGAGACTGAATCATCATCGAAGAACACCTTGAGCGGTTTCCCTGTAGCAGATAGACGCCGCAGTTGGGGAGGATTTAGGTGTTTTGGTCAAGATGGATCGACGAACCAATTCTCATCCTCTGACCTCATCAATATTATTGTATCTGTTGAGGACACAGGAGTAGGAATCCCTCTAGAAGCACAATCTCGTGTTTTCACTCCTTTTATGCAGGTGGGACCATCCATCTCACGTACACATGGAGGCACAGGAATTGGGCTAAGCATAAGCAAGTGTTTGGTTGGCCTCATGAAAGGGGAAATCGGGTTTGTGAGCATTCCCAAGATTGGTTCCACTTTTACATTTACTGCTGTTTTCACCAATGTCAGCTCCAATTCAAACGAGTTAACAATCCAGCAAATGAACAGCCAGTCAAATGCTGCATCCTCAGAATTTAATGGCATGACGGCATTAGTAGTGGACCAAAGACCTGTTAGAGCCAAGATGTCAAGATATCATATCCAACGGCTTGGAATTTGTGTTGAAGTAGTTTCTGATTTGAATCAGGGTTTATCTAGCATAATCTCTGGGAATACCACCATCAATATGGTCCTTGTTGAACAGGAAGTCTGGGACAAAGATTCGAGCACCTCAGCTCTCTTTGTCAATAATTTACGGAAAATTGATGGACAGGTTCCTCTAAAACTGTTCATTCTCACCAATTCTATAAGCTCATGCAGAATAAGTTCTGCAACTTCAGGTGTCTCTACCCCAACTGTCATCATGAAGCCTCTCAGGGCAAGCATGCTTGCTGCCTCACTACAACGAGCCATGGGAGTTGGTAACAAGGGAAATCTCCGGAATGGGGACCTCCCAAGTTTGTCTCTCCGCAATCTTCTGCTCGGGAGAAAAATTCTTATTATAGATGATAATAATGTGAATCTCAGAGTAGCTGCTGGTGCTTTGAAGAAGTATGGGGCTGAGGTGGTCTGTGCAGACAGTGGTAAAAAGGCCATCTCACTGCTTACGCCGCCCCACCATTTTGATGCCTGCTTCATGGATATCCAGATGCCAGAGATGGACGGGTTTGAAGCTACAAGGAGAATACGCAACATGGAATGCAATATCAGTAACCATATTCAGCATGGCGAAGTTTCTGCAGAGGATTATGAAAATATTCAAGCATGGCATGTACCCATTCTGGCCATGACTGCAGACGTAATTCAGGCAACCCATGAGGAATGTACAAGGTGCGGAATGGATGGATATGTTTCAAAACCATTCGAAGCTGAACAGCTTTATCGCGAAGTTTCACGTTTTTTCCAATCTACAACAACTGGGAGCTTGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004673 GO:0004888 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006970 GO:0007154 GO:0007165 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009266 GO:0009267 GO:0009409 GO:0009414 GO:0009415 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009735 GO:0009736 GO:0009737 GO:0009743 GO:0009744 GO:0009755 GO:0009884 GO:0009888 GO:0009894 GO:0009909 GO:0009987 GO:0009991 GO:0010029 GO:0010033 GO:0010035 GO:0010087 GO:0010150 GO:0010271 GO:0010959 GO:0012505 GO:0016020 GO:0016036 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0016775 GO:0018106 GO:0018193 GO:0018202 GO:0019222 GO:0019538 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0031323 GO:0031329 GO:0031667 GO:0031668 GO:0031669 GO:0031984 GO:0032501 GO:0032502 GO:0032870 GO:0032879 GO:0033554 GO:0033993 GO:0034285 GO:0034756 GO:0034757 GO:0036211 GO:0038023 GO:0040007 GO:0042175 GO:0042221 GO:0042594 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043269 GO:0043271 GO:0043412 GO:0043424 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0048366 GO:0048367 GO:0048509 GO:0048519 GO:0048580 GO:0048731 GO:0048827 GO:0048831 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051049 GO:0051051 GO:0051171 GO:0051193 GO:0051239 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0070417 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071368 GO:0071396 GO:0071495 GO:0071496 GO:0071704 GO:0071944 GO:0080117 GO:0080190 GO:0090056 GO:0090693 GO:0097305 GO:0097306 GO:0098542 GO:0098827 GO:0099402 GO:0140096 GO:1900140 GO:1901401 GO:1901404 GO:1901564 GO:1901700 GO:1901701 GO:2000026 GO:2000241
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
CHASE HATPase_c HisKA Response_reg

Protein Analysis

1040

Amino Acids

116.04

Weight (kDa)

7.85

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CHASE PF03924 160 - 356 5.7e-37 CHASE domain
HisKA PF00512 444 - 509 4.7e-18 His Kinase A (phospho-acceptor) domain
HATPase_c PF02518 641 - 713 3.9e-14 Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase
Receiver_CRE1 PF24896 738 - 868 1.4e-43 AHK4/CRE1/WOL first receiver domain
Response_reg PF00072 894 - 1025 4.4e-19 Response regulator receiver domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014276)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27320
fragaria_vesca FvH4_4g23590 FvH4_4g23590 FvH4_4g23590 FvH4_4g23590
malus_domestica MD13G1120900.v1.1 MD16G1068400.v1.1 MD16G1120900.v1.1
prunus_persica Prupe.1G224300_v2.0.a1
pyrus_communis pycom13g10520
rosa_chinensis RchiOBHm_Chr4g0430011
rosa_laevigata RLG00000007014
rosa_roxburghii Rroxscaffold_5G00371820
rosa_rugosa Rorug04G0239800
rosa_samantha Rh4BG302900 Rh4CG318400
rosa_wichuraiana Rw4G025680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1490
AarI CACCTGC 1 cut(s) 1990
Acc36I ACCTGC 2 cut(s) 1143, 1990
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 1 cut(s) 93
AccBSI CCGCTC 1 cut(s) 1824
AccI GTMKAC 2 cut(s) 882, 1632
AccII CGCG 2 cut(s) 502, 3072
AccIII TCCGGA 1 cut(s) 2625
AciI CCGC 9 cut(s) 205, 502, 853, 1147, 1824, 1849, 2656, 2798, 3021
AclWI GGATC 6 cut(s) 196, 317, 405, 1118, 1664, 1894
AcoI YGGCCR 2 cut(s) 1175, 2970
AcsI RAATTY 6 cut(s) 924, 1711, 1721, 2219, 2301, 2679
AcuI CTGAAG 1 cut(s) 2508
AcyI GRCGYC 1 cut(s) 1846
AfaI GTAC 7 cut(s) 98, 320, 470, 506, 2023, 2961, 3013
AfiI CCNNNNNNNGG 7 cut(s) 93, 427, 694, 1382, 1855, 2114, 2632
AflII CTTAAG 2 cut(s) 1111, 1129
AflIII ACRYGT 2 cut(s) 786, 1026
AjnI CCWGG 3 cut(s) 642, 1458, 1506
AloI GAACNNNNNNTCC 2 cut(s) 801, 833
Alw21I GWGCWC 4 cut(s) 329, 570, 1302, 2417
Alw26I GTCTC 7 cut(s) 347, 523, 1486, 1608, 1787, 2536, 2655
AlwI GGATC 6 cut(s) 196, 317, 405, 1118, 1664, 1894
Ama87I CYCGRG 1 cut(s) 2669
Aor13HI TCCGGA 1 cut(s) 2625
AoxI GGCC 5 cut(s) 429, 1175, 2071, 2778, 2970
ApeKI GCWGC 8 cut(s) 149, 557, 598, 1096, 1319, 2207, 2579, 2720
ApoI RAATTY 6 cut(s) 924, 1711, 1721, 2219, 2301, 2679
Asp700I GAANNNNTTC 4 cut(s) 924, 1898, 2512, 2943
Asp718I GGTACC 1 cut(s) 96
AspA2I CCTAGG 1 cut(s) 1390
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 5 cut(s) 1504, 2006, 2248, 2377, 2635
AsuC2I CCSGG 2 cut(s) 766, 1674
AsuHPI GGTGA 5 cut(s) 155, 1679, 1687, 2137, 2476
AsuII TTCGAA 1 cut(s) 3051
AsuNHI GCTAGC 1 cut(s) 1001
AvaI CYCGRG 1 cut(s) 2669
AvaII GGWCC 5 cut(s) 1504, 2006, 2248, 2377, 2635
AvrII CCTAGG 1 cut(s) 1390
BalI TGGCCA 1 cut(s) 2972
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 2 cut(s) 329, 1302
BauI CACGAG 3 cut(s) 1211, 1361, 1977
Bbv12I GWGCWC 4 cut(s) 329, 570, 1302, 2417
BbvCI CCTCAGC 3 cut(s) 1314, 2419, 2748
BbvI GCAGC 8 cut(s) 136, 544, 610, 1108, 1331, 2194, 2566, 2707
BceAI ACGGC 3 cut(s) 1162, 2250, 2309
BcgI CGANNNNNNTGC 4 cut(s) 1338, 1372, 2906, 2940
BciT130I CCWGG 3 cut(s) 644, 1460, 1508
BciVI GTATCC 3 cut(s) 619, 721, 1226
BclI TGATCA 1 cut(s) 484
BcnI CCSGG 2 cut(s) 766, 1674
BcoDI GTCTC 7 cut(s) 347, 523, 1486, 1608, 1787, 2536, 2655
BfaI CTAG 8 cut(s) 336, 384, 798, 1002, 1391, 1439, 1964, 2343
BfmI CTRYAG 3 cut(s) 1833, 2925, 2979
BfrI CTTAAG 2 cut(s) 1111, 1129
BfuAI ACCTGC 2 cut(s) 1143, 1990
BfuI GTATCC 3 cut(s) 619, 721, 1226
BglI GCCNNNNNGGC 1 cut(s) 1174
BglII AGATCT 1 cut(s) 892
BlnI CCTAGG 1 cut(s) 1390
BlpI GCTNAGC 1 cut(s) 2048
Bme1390I CCNGG 5 cut(s) 644, 766, 1460, 1508, 1674
Bme18I GGWCC 5 cut(s) 1504, 2006, 2248, 2377, 2635
BmeT110I CYCGRG 1 cut(s) 2669
BmgT120I GGNCC 5 cut(s) 1504, 2006, 2248, 2377, 2635
BmiI GGNNCC 9 cut(s) 98, 302, 681, 1136, 1650, 2007, 2119, 2463, 2636
BmrFI CCNGG 5 cut(s) 644, 766, 1460, 1508, 1674
BmrI ACTGGG 2 cut(s) 683, 3114
BmsI GCATC 7 cut(s) 45, 52, 1060, 1134, 2219, 2803, 2826
BmtI GCTAGC 1 cut(s) 1005
BmuI ACTGGG 2 cut(s) 683, 3114
BpmI CTGGAG 1 cut(s) 1553
Bpu10I CCTNAGC 3 cut(s) 1314, 2419, 2748
Bpu1102I GCTNAGC 1 cut(s) 2048
Bpu14I TTCGAA 1 cut(s) 3051
BpuEI CTTGAG 4 cut(s) 1286, 1559, 1590, 1840
BpuMI CCSGG 2 cut(s) 766, 1674
BsaAI YACGTR 2 cut(s) 276, 2021
BsaBI GATNNNNATC 2 cut(s) 443, 2328
BsaHI GRCGYC 1 cut(s) 1846
BsaI GGTCTC 1 cut(s) 523
BsaWI WCCGGW 1 cut(s) 2625
BsaXI ACNNNNNCTCC 2 cut(s) 1091, 1121
Bsc4I CCNNNNNNNGG 7 cut(s) 93, 427, 694, 1382, 1855, 2114, 2632
Bse1I ACTGG 6 cut(s) 98, 109, 220, 689, 2197, 3109
Bse8I GATNNNNATC 2 cut(s) 443, 2328
BseAI TCCGGA 1 cut(s) 2625
BseBI CCWGG 3 cut(s) 644, 1460, 1508
BseJI GATNNNNATC 2 cut(s) 443, 2328
BseLI CCNNNNNNNGG 7 cut(s) 93, 427, 694, 1382, 1855, 2114, 2632
BseMII CTCAG 8 cut(s) 1305, 1352, 1653, 2229, 2433, 2575, 2725, 2739
BseNI ACTGG 6 cut(s) 98, 109, 220, 689, 2197, 3109
BseRI GAGGAG 1 cut(s) 1781
BseXI GCAGC 8 cut(s) 136, 544, 610, 1108, 1331, 2194, 2566, 2707
BsgI GTGCAG 2 cut(s) 1171, 2781
Bsh1236I CGCG 2 cut(s) 502, 3072
BshFI GGCC 5 cut(s) 431, 1177, 2073, 2780, 2972
BshNI GGYRCC 1 cut(s) 96
BsiHKAI GWGCWC 4 cut(s) 329, 570, 1302, 2417
BsiHKCI CYCGRG 1 cut(s) 2669
BsiSI CCGG 3 cut(s) 765, 1674, 2626
BslFI GGGAC 3 cut(s) 2019, 2414, 2648
BslI CCNNNNNNNGG 7 cut(s) 93, 427, 694, 1382, 1855, 2114, 2632
BsmAI GTCTC 7 cut(s) 347, 523, 1486, 1608, 1787, 2536, 2655
BsmFI GGGAC 3 cut(s) 2019, 2414, 2648
BsmI GAATGC 2 cut(s) 2103, 2891
BsnI GGCC 5 cut(s) 431, 1177, 2073, 2780, 2972
Bso31I GGTCTC 1 cut(s) 523
BsoBI CYCGRG 1 cut(s) 2669
Bsp119I TTCGAA 1 cut(s) 3051
Bsp1286I GDGCHC 4 cut(s) 329, 570, 1302, 2417
Bsp13I TCCGGA 1 cut(s) 2625
Bsp1407I TGTACA 1 cut(s) 3011
Bsp1720I GCTNAGC 1 cut(s) 2048
Bsp19I CCATGG 2 cut(s) 1172, 2598
Bsp68I TCGCGA 1 cut(s) 3072
BspACI CCGC 9 cut(s) 205, 502, 853, 1147, 1824, 1849, 2656, 2798, 3021
BspANI GGCC 5 cut(s) 431, 1177, 2073, 2780, 2972
BspCNI CTCAG 8 cut(s) 1306, 1351, 1652, 2228, 2432, 2574, 2724, 2740
BspEI TCCGGA 1 cut(s) 2625
BspFNI CGCG 2 cut(s) 502, 3072
BspHI TCATGA 2 cut(s) 2076, 2550
BspLI GGNNCC 9 cut(s) 98, 302, 681, 1136, 1650, 2007, 2119, 2463, 2636
BspMAI CTGCAG 2 cut(s) 2929, 2983
BspMI ACCTGC 2 cut(s) 1143, 1990
BspOI GCTAGC 1 cut(s) 1005
BspPI GGATC 6 cut(s) 196, 317, 405, 1118, 1664, 1894
BspT104I TTCGAA 1 cut(s) 3051
BspT107I GGYRCC 1 cut(s) 96
BspTI CTTAAG 2 cut(s) 1111, 1129
BspTNI GGTCTC 1 cut(s) 523
BsrBI CCGCTC 1 cut(s) 1824
BsrGI TGTACA 1 cut(s) 3011
BsrI ACTGG 6 cut(s) 98, 109, 220, 689, 2197, 3109
BssNAI GTATAC 1 cut(s) 1633
BssNI GRCGYC 1 cut(s) 1846
BssSI CACGAG 3 cut(s) 1211, 1361, 1977
BssT1I CCWWGG 6 cut(s) 126, 675, 856, 1172, 1390, 2598
Bst1107I GTATAC 1 cut(s) 1633
Bst2BI CACGAG 3 cut(s) 1211, 1361, 1977
Bst2UI CCWGG 3 cut(s) 644, 1460, 1508
Bst4CI ACNGT 7 cut(s) 733, 1252, 1354, 1753, 2475, 2545, 2768
Bst6I CTCTTC 2 cut(s) 230, 338
BstACI GRCGYC 1 cut(s) 1846
BstAFI CTTAAG 2 cut(s) 1111, 1129
BstAPI GCANNNNNTGC 1 cut(s) 557
BstAUI TGTACA 1 cut(s) 3011
BstBAI YACGTR 2 cut(s) 276, 2021
BstBI TTCGAA 1 cut(s) 3051
BstC8I GCNNGC 8 cut(s) 795, 1003, 1007, 1563, 2569, 2573, 2577, 2818
BstDEI CTNAG 9 cut(s) 1314, 1338, 1639, 2048, 2215, 2419, 2561, 2711, 2748
BstDSI CCRYGG 4 cut(s) 460, 1172, 1178, 2598
BstFNI CGCG 2 cut(s) 502, 3072
BstHHI GCGC 1 cut(s) 502
BstMAI GTCTC 7 cut(s) 347, 523, 1486, 1608, 1787, 2536, 2655
BstNI CCWGG 3 cut(s) 644, 1460, 1508
BstNSI RCATGY 3 cut(s) 1066, 2575, 2960
BstSCI CCNGG 5 cut(s) 642, 764, 1458, 1506, 1672
BstSFI CTRYAG 3 cut(s) 1833, 2925, 2979
BstUI CGCG 2 cut(s) 502, 3072
BstV1I GCAGC 8 cut(s) 136, 544, 610, 1108, 1331, 2194, 2566, 2707
BstX2I RGATCY 4 cut(s) 201, 309, 892, 1123
BstXI CCANNNNNNTGG 5 cut(s) 20, 1179, 1466, 2297, 2374
BstYI RGATCY 4 cut(s) 201, 309, 892, 1123
BstZ17I GTATAC 1 cut(s) 1633
BsuI GTATCC 3 cut(s) 619, 721, 1226
BsuRI GGCC 5 cut(s) 431, 1177, 2073, 2780, 2972
BtgI CCRYGG 4 cut(s) 460, 1172, 1178, 2598
BtsI GCAGTG 1 cut(s) 2786
BtsIMutI CAGTG 4 cut(s) 102, 507, 2773, 2786
BtuMI TCGCGA 1 cut(s) 3072
BveI ACCTGC 2 cut(s) 1143, 1990
Cac8I GCNNGC 8 cut(s) 795, 1003, 1007, 1563, 2569, 2573, 2577, 2818
CciI TCATGA 2 cut(s) 2076, 2550
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 5 cut(s) 1504, 2006, 2248, 2377, 2635
CseI GACGC 1 cut(s) 1854
CsiI ACCWGGT 1 cut(s) 642
Csp6I GTAC 7 cut(s) 97, 319, 469, 505, 2022, 2960, 3012
CspCI CAANNNNNGTGG 2 cut(s) 528, 563
CviQI GTAC 7 cut(s) 97, 319, 469, 505, 2022, 2960, 3012
DdeI CTNAG 9 cut(s) 1314, 1338, 1639, 2048, 2215, 2419, 2561, 2711, 2748
DraI TTTAAA 1 cut(s) 33
EaeI YGGCCR 2 cut(s) 1175, 2970
Eam1104I CTCTTC 2 cut(s) 230, 338
EarI CTCTTC 2 cut(s) 230, 338
Ecl136II GAGCTC 2 cut(s) 327, 1300
Eco130I CCWWGG 6 cut(s) 126, 675, 856, 1172, 1390, 2598
Eco24I GRGCYC 2 cut(s) 329, 1302
Eco31I GGTCTC 1 cut(s) 523
Eco32I GATATC 2 cut(s) 2282, 2830
Eco47I GGWCC 5 cut(s) 1504, 2006, 2248, 2377, 2635
Eco53kI GAGCTC 2 cut(s) 327, 1300
Eco57I CTGAAG 1 cut(s) 2508
Eco88I CYCGRG 1 cut(s) 2669
EcoICRI GAGCTC 2 cut(s) 327, 1300
EcoO109I RGGNCCY 1 cut(s) 2635
EcoRI GAATTC 2 cut(s) 924, 1711
EcoRII CCWGG 3 cut(s) 642, 1458, 1506
EcoRV GATATC 2 cut(s) 2282, 2830
EcoT14I CCWWGG 6 cut(s) 126, 675, 856, 1172, 1390, 2598
EcoT38I GRGCYC 2 cut(s) 329, 1302
ErhI CCWWGG 6 cut(s) 126, 675, 856, 1172, 1390, 2598
FalI AAGNNNNNCTT 4 cut(s) 505, 537, 1802, 1834
FaqI GGGAC 3 cut(s) 2019, 2414, 2648
FauNDI CATATG 2 cut(s) 553, 1404
FbaI TGATCA 1 cut(s) 484
FblI GTMKAC 2 cut(s) 882, 1632
FriOI GRGCYC 2 cut(s) 329, 1302
FspBI CTAG 8 cut(s) 336, 384, 798, 1002, 1391, 1439, 1964, 2343
GlaI GCGC 1 cut(s) 501
GsuI CTGGAG 1 cut(s) 1553
HaeIII GGCC 5 cut(s) 431, 1177, 2073, 2780, 2972
HapII CCGG 3 cut(s) 765, 1674, 2626
HgaI GACGC 1 cut(s) 1854
HhaI GCGC 1 cut(s) 502
Hin1I GRCGYC 1 cut(s) 1846
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 1 cut(s) 2175
HindII GTYRAC 1 cut(s) 2175
HpaI GTTAAC 1 cut(s) 2175
HpaII CCGG 3 cut(s) 765, 1674, 2626
HphI GGTGA 5 cut(s) 155, 1679, 1687, 2137, 2476
Hpy166II GTNNAC 8 cut(s) 273, 507, 883, 1024, 1633, 2024, 2175, 2248
Hpy8I GTNNAC 8 cut(s) 273, 507, 883, 1024, 1633, 2024, 2175, 2248
Hpy99I CGWCG 1 cut(s) 1894
HpyAV CCTTC 4 cut(s) 253, 343, 763, 1671
HpyCH4III ACNGT 7 cut(s) 733, 1252, 1354, 1753, 2475, 2545, 2768
HpyCH4IV ACGT 6 cut(s) 275, 786, 1026, 2020, 2985, 3082
HpyF3I CTNAG 9 cut(s) 1314, 1338, 1639, 2048, 2215, 2419, 2561, 2711, 2748
HpySE526I ACGT 6 cut(s) 275, 786, 1026, 2020, 2985, 3082
Hsp92I GRCGYC 1 cut(s) 1846
HspAI GCGC 1 cut(s) 500
Kpn2I TCCGGA 1 cut(s) 2625
KpnI GGTACC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 484
KspAI GTTAAC 1 cut(s) 2175
Lsp1109I GCAGC 8 cut(s) 136, 544, 610, 1108, 1331, 2194, 2566, 2707
LweI GCATC 7 cut(s) 45, 52, 1060, 1134, 2219, 2803, 2826
MabI ACCWGGT 1 cut(s) 642
MaeI CTAG 8 cut(s) 336, 384, 798, 1002, 1391, 1439, 1964, 2343
MaeII ACGT 6 cut(s) 275, 786, 1026, 2020, 2985, 3082
MaeIII GTNAC 9 cut(s) 115, 969, 984, 1398, 1429, 1584, 1747, 2609, 2897
MbiI CCGCTC 1 cut(s) 1824
MboII GAAGA 7 cut(s) 247, 355, 710, 785, 1822, 2654, 2746
MfeI CAATTG 1 cut(s) 294
MflI RGATCY 4 cut(s) 201, 309, 892, 1123
MhlI GDGCHC 4 cut(s) 329, 570, 1302, 2417
MlsI TGGCCA 1 cut(s) 2972
MluNI TGGCCA 1 cut(s) 2972
MlyI GAGTC 4 cut(s) 249, 349, 977, 1514
MmeI TCCRAC 4 cut(s) 265, 1210, 1482, 2314
Mox20I TGGCCA 1 cut(s) 2972
MroI TCCGGA 1 cut(s) 2625
MroXI GAANNNNTTC 4 cut(s) 924, 1898, 2512, 2943
MscI TGGCCA 1 cut(s) 2972
MseI TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2174, 2223
MslI CAYNNNNRTG 5 cut(s) 654, 1019, 1177, 2372, 2811
Msp20I TGGCCA 1 cut(s) 2972
MspA1I CMGCKG 1 cut(s) 1322
MspCI CTTAAG 2 cut(s) 1111, 1129
MspI CCGG 3 cut(s) 765, 1674, 2626
MspR9I CCNGG 5 cut(s) 644, 766, 1460, 1508, 1674
MunI CAATTG 1 cut(s) 294
Mva1269I GAATGC 2 cut(s) 2103, 2891
MvaI CCWGG 3 cut(s) 644, 1460, 1508
MvnI CGCG 2 cut(s) 502, 3072
NciI CCSGG 2 cut(s) 766, 1674
NcoI CCATGG 2 cut(s) 1172, 2598
NdeI CATATG 2 cut(s) 553, 1404
NheI GCTAGC 1 cut(s) 1001
NlaIV GGNNCC 9 cut(s) 98, 302, 681, 1136, 1650, 2007, 2119, 2463, 2636
NmuCI GTSAC 4 cut(s) 115, 969, 1584, 1747
NruI TCGCGA 1 cut(s) 3072
NspI RCATGY 3 cut(s) 1066, 2575, 2960
NspV TTCGAA 1 cut(s) 3051
PaeI GCATGC 1 cut(s) 2575
PagI TCATGA 2 cut(s) 2076, 2550
PaqCI CACCTGC 1 cut(s) 1990
PasI CCCWGGG 1 cut(s) 1459
PctI GAATGC 2 cut(s) 2103, 2891
PdmI GAANNNNTTC 4 cut(s) 924, 1898, 2512, 2943
PfeI GAWTC 8 cut(s) 803, 954, 1206, 1799, 1956, 2329, 2408, 2707
PflMI CCANNNNNTGG 1 cut(s) 93
PfoI TCCNGGA 1 cut(s) 1672
PleI GAGTC 4 cut(s) 248, 349, 976, 1514
PpsI GAGTC 4 cut(s) 248, 349, 976, 1514
Ppu21I YACGTR 2 cut(s) 276, 2021
PpuMI RGGWCCY 1 cut(s) 2635
PsiI TTATAA 1 cut(s) 1490
Psp124BI GAGCTC 2 cut(s) 329, 1302
Psp5II RGGWCCY 1 cut(s) 2635
Psp6I CCWGG 3 cut(s) 642, 1458, 1506
PspGI CCWGG 3 cut(s) 642, 1458, 1506
PspN4I GGNNCC 9 cut(s) 98, 302, 681, 1136, 1650, 2007, 2119, 2463, 2636
PspPI GGNCC 5 cut(s) 1504, 2006, 2248, 2377, 2635
PspPPI RGGWCCY 1 cut(s) 2635
PstI CTGCAG 2 cut(s) 2929, 2983
PsuI RGATCY 4 cut(s) 201, 309, 892, 1123
PvuII CAGCTG 1 cut(s) 1322
RruI TCGCGA 1 cut(s) 3072
RsaI GTAC 7 cut(s) 98, 320, 470, 506, 2023, 2961, 3013
RsaNI GTAC 7 cut(s) 97, 319, 469, 505, 2022, 2960, 3012
RseI CAYNNNNRTG 5 cut(s) 654, 1019, 1177, 2372, 2811
SacI GAGCTC 2 cut(s) 329, 1302
SaqAI TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2174, 2223
Sau96I GGNCC 5 cut(s) 1504, 2006, 2248, 2377, 2635
SchI GAGTC 4 cut(s) 249, 349, 977, 1514
ScrFI CCNGG 5 cut(s) 644, 766, 1460, 1508, 1674
SduI GDGCHC 4 cut(s) 329, 570, 1302, 2417
SexAI ACCWGGT 1 cut(s) 642
SfaNI GCATC 7 cut(s) 45, 52, 1060, 1134, 2219, 2803, 2826
SfcI CTRYAG 3 cut(s) 1833, 2925, 2979
SfuI TTCGAA 1 cut(s) 3051
SinI GGWCC 5 cut(s) 1504, 2006, 2248, 2377, 2635
SmiMI CAYNNNNRTG 5 cut(s) 654, 1019, 1177, 2372, 2811
SmlI CTYRAG 6 cut(s) 1111, 1129, 1301, 1538, 1605, 1819
SmoI CTYRAG 6 cut(s) 1111, 1129, 1301, 1538, 1605, 1819
SphI GCATGC 1 cut(s) 2575
SsiI CCGC 9 cut(s) 205, 502, 853, 1147, 1824, 1849, 2656, 2798, 3021
SspI AATATT 2 cut(s) 1924, 2944
SspMI CTAG 8 cut(s) 336, 384, 798, 1002, 1391, 1439, 1964, 2343
SstI GAGCTC 2 cut(s) 329, 1302
StyD4I CCNGG 5 cut(s) 642, 764, 1458, 1506, 1672
StyI CCWWGG 6 cut(s) 126, 675, 856, 1172, 1390, 2598
TaaI ACNGT 7 cut(s) 733, 1252, 1354, 1753, 2475, 2545, 2768
TaiI ACGT 6 cut(s) 278, 789, 1029, 2023, 2988, 3085
TatI WGTACW 1 cut(s) 3011
TauI GCSGC 2 cut(s) 1851, 2800
TfiI GAWTC 8 cut(s) 803, 954, 1206, 1799, 1956, 2329, 2408, 2707
Tru1I TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2174, 2223
Tru9I TTAA 7 cut(s) 32, 405, 843, 1112, 1130, 2174, 2223
TscAI CASTG 4 cut(s) 109, 514, 2773, 2793
TseFI GTSAC 4 cut(s) 115, 969, 1584, 1747
TseI GCWGC 8 cut(s) 149, 557, 598, 1096, 1319, 2207, 2579, 2720
Tsp45I GTSAC 4 cut(s) 115, 969, 1584, 1747
TspGWI ACGGA 2 cut(s) 1274, 2458
TspRI CASTG 4 cut(s) 109, 514, 2773, 2793
Van91I CCANNNNNTGG 1 cut(s) 93
Vha464I CTTAAG 2 cut(s) 1111, 1129
VpaK11BI GGWCC 5 cut(s) 1504, 2006, 2248, 2377, 2635
XapI RAATTY 6 cut(s) 924, 1711, 1721, 2219, 2301, 2679
XbaI TCTAGA 1 cut(s) 1963
XceI RCATGY 3 cut(s) 1066, 2575, 2960
XcmI CCANNNNNNNNNTGG 3 cut(s) 1176, 2371, 2909
XmaJI CCTAGG 1 cut(s) 1390
XmiI GTMKAC 2 cut(s) 882, 1632
XmnI GAANNNNTTC 4 cut(s) 924, 1898, 2512, 2943
XspI CTAG 8 cut(s) 336, 384, 798, 1002, 1391, 1439, 1964, 2343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.