AT1G27530

E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011875
Physical Location & Seq
Reverse (-)
9562487 .. 9564031
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27530.1

Sequence Viewer

Length: 525 bp
ATGGAAGGATGGGATCCGAACACGAAGTCGACGTTAACGCGAATACCGCTTCTGACGACAAAGGCGGGTCCAAGAGACGGCGCGGCATGGACGCAGAGGCTGAAAGAGGAGTACAAGTCTCTGATTGCGTATACCCAAATGAACAAGTCCAACGACAATGACTGGTTCCGTATCTCCGCTTCCAATCCTGAGGGCACGCGTTGGACTGGCAAGTGTTGGTATGTTCACAACCTTCTCAAATACGAATTCGATCTCCAGTTCGATATCCCGATTACCTATCCAGCCACTGCTCCTGAGCTTGAGCTGCCTGAGATTGACGGCAAGACTCAAAAGATGTATCGAGGTGGGAAGATATGTTTGACTGTGCATTTCAAGCCTCTATGGGCCAAAAACTGTCCTCGGTTTGGTATAGCACATGCACTTTGTTTGGGGCTCGCTCCGTGGTTAGCTGCAGAGATTCCAATTCTTGTGGACTCCGGCGCGATTAAGCACAAAGATGACGCAGCCACATCTGCGGAATCTTAA

Protein Analysis

174

Amino Acids

19.61

Weight (kDa)

7.66

Isoelectric Point (pI)

29.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFC1 PF08694 8 - 163 2.2e-84 Ubiquitin-fold modifier-conjugating enzyme 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27530
fragaria_vesca FvH4_5g33720 FvH4_5g33720
malus_domestica MD05G1007500.v1.1 MD08G1209200.v1.1 MD15G1393200.v1.1
prunus_persica Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1
pyrus_communis pycom05g10270 pycom08g18060 pycom15g35350
rosa_chinensis RchiOBHm_Chr7g0232851
rosa_laevigata RLG00000001313
rosa_multiflora Rmu_ssc0000018.1_g000017
rosa_roxburghii Rroxscaffold_3G00227850
rosa_rugosa Rorug07G0276400
rosa_samantha Rh7CG450300
rosa_wichuraiana Rw7G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 29, 131
AccII CGCG 4 cut(s) 40, 83, 199, 482
AciI CCGC 5 cut(s) 47, 65, 83, 177, 515
AclWI GGATC 2 cut(s) 8, 21
AcsI RAATTY 1 cut(s) 245
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 2 cut(s) 77, 404
AflIII ACRYGT 1 cut(s) 197
AgsI TTSAA 1 cut(s) 373
AluBI AGCT 3 cut(s) 298, 304, 449
AluI AGCT 3 cut(s) 298, 304, 449
Alw26I GTCTC 2 cut(s) 69, 123
AlwI GGATC 2 cut(s) 8, 21
AlwNI CAGNNNCTG 2 cut(s) 100, 287
AoxI GGCC 1 cut(s) 384
ApeKI GCWGC 3 cut(s) 304, 449, 503
ApoI RAATTY 1 cut(s) 245
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AspLEI GCGC 2 cut(s) 83, 482
AspS9I GGNCC 2 cut(s) 68, 384
AvaII GGWCC 1 cut(s) 68
AxyI CCTNAGG 1 cut(s) 189
BaeGI GKGCMC 1 cut(s) 197
BamHI GGATCC 1 cut(s) 13
BanII GRGCYC 1 cut(s) 435
BarI GAAGNNNNNNTAC 2 cut(s) 163, 195
BbvI GCAGC 3 cut(s) 291, 436, 515
BccI CCATC 1 cut(s) 3
BceAI ACGGC 2 cut(s) 94, 334
BcoDI GTCTC 2 cut(s) 69, 123
BfmI CTRYAG 1 cut(s) 450
BisI GCNGC 4 cut(s) 84, 305, 450, 504
BlsI GCNGC 4 cut(s) 85, 306, 451, 505
Bme18I GGWCC 1 cut(s) 68
BmgT120I GGNCC 2 cut(s) 68, 384
BmiI GGNNCC 3 cut(s) 15, 69, 167
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 320
BsaJI CCNNGG 2 cut(s) 398, 440
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 404
Bse1I ACTGG 3 cut(s) 167, 211, 256
Bse21I CCTNAGG 1 cut(s) 189
BseDI CCNNGG 2 cut(s) 398, 440
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 2 cut(s) 77, 404
BseMII CTCAG 3 cut(s) 180, 285, 300
BseNI ACTGG 3 cut(s) 167, 211, 256
BseRI GAGGAG 1 cut(s) 122
BseSI GKGCMC 1 cut(s) 197
BseXI GCAGC 3 cut(s) 291, 436, 515
Bsh1236I CGCG 4 cut(s) 40, 83, 199, 482
BshFI GGCC 1 cut(s) 386
BsiSI CCGG 1 cut(s) 477
BslI CCNNNNNNNGG 2 cut(s) 77, 404
BsmAI GTCTC 2 cut(s) 69, 123
BsmBI CGTCTC 1 cut(s) 69
BsnI GGCC 1 cut(s) 386
Bsp1286I GDGCHC 2 cut(s) 197, 435
Bsp143I GATC 2 cut(s) 13, 250
BspACI CCGC 5 cut(s) 47, 65, 83, 177, 515
BspANI GGCC 1 cut(s) 386
BspCNI CTCAG 3 cut(s) 181, 286, 301
BspFNI CGCG 4 cut(s) 40, 83, 199, 482
BspLI GGNNCC 3 cut(s) 15, 69, 167
BspMAI CTGCAG 1 cut(s) 454
BspPI GGATC 2 cut(s) 8, 21
BsrI ACTGG 3 cut(s) 167, 211, 256
BssECI CCNNGG 2 cut(s) 398, 440
BssMI GATC 2 cut(s) 13, 250
BssNAI GTATAC 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 132
Bst4CI ACNGT 2 cut(s) 364, 395
BstC8I GCNNGC 2 cut(s) 197, 435
BstDEI CTNAG 3 cut(s) 189, 294, 309
BstDSI CCRYGG 1 cut(s) 440
BstF5I GGATG 1 cut(s) 14
BstFNI CGCG 4 cut(s) 40, 83, 199, 482
BstHHI GCGC 2 cut(s) 83, 482
BstKTI GATC 2 cut(s) 16, 253
BstMAI GTCTC 2 cut(s) 69, 123
BstMBI GATC 2 cut(s) 13, 250
BstMWI GCNNNNNNNGC 4 cut(s) 46, 304, 373, 512
BstNSI RCATGY 1 cut(s) 419
BstSFI CTRYAG 1 cut(s) 450
BstSLI GKGCMC 1 cut(s) 197
BstUI CGCG 4 cut(s) 40, 83, 199, 482
BstV1I GCAGC 3 cut(s) 291, 436, 515
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BstZ17I GTATAC 1 cut(s) 132
Bsu36I CCTNAGG 1 cut(s) 189
BsuRI GGCC 1 cut(s) 386
BtgI CCRYGG 1 cut(s) 440
BtsCI GGATG 1 cut(s) 14
BtsI GCAGTG 1 cut(s) 285
BtsIMutI CAGTG 1 cut(s) 285
Cac8I GCNNGC 2 cut(s) 197, 435
CaiI CAGNNNCTG 2 cut(s) 100, 287
CfoI GCGC 2 cut(s) 83, 482
Cfr13I GGNCC 2 cut(s) 68, 384
CseI GACGC 2 cut(s) 100, 509
Csp6I GTAC 1 cut(s) 112
CspCI CAANNNNNGTGG 2 cut(s) 450, 485
CviAII CATG 2 cut(s) 87, 416
CviJI RGCY 9 cut(s) 100, 284, 298, 304, 376, 386, 433, 449, 506
CviKI_1 RGCY 9 cut(s) 100, 284, 298, 304, 376, 386, 433, 449, 506
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 3 cut(s) 189, 294, 309
DpnI GATC 2 cut(s) 15, 252
DpnII GATC 2 cut(s) 13, 250
Eco24I GRGCYC 1 cut(s) 435
Eco32I GATATC 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 68
Eco81I CCTNAGG 1 cut(s) 189
EcoRI GAATTC 1 cut(s) 245
EcoRV GATATC 1 cut(s) 265
EcoT38I GRGCYC 1 cut(s) 435
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 2 cut(s) 90, 419
FaiI YATR 7 cut(s) 88, 132, 222, 355, 382, 410, 417
FatI CATG 2 cut(s) 86, 415
FauI CCCGC 1 cut(s) 58
FblI GTMKAC 2 cut(s) 29, 131
Fnu4HI GCNGC 4 cut(s) 84, 305, 450, 504
FokI GGATG 1 cut(s) 21
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 4 cut(s) 84, 305, 450, 504
GlaI GCGC 2 cut(s) 82, 481
GluI GCNGC 4 cut(s) 84, 305, 450, 504
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 1 cut(s) 386
HapII CCGG 1 cut(s) 477
HgaI GACGC 2 cut(s) 100, 509
HhaI GCGC 2 cut(s) 83, 482
Hin1II CATG 2 cut(s) 90, 419
Hin6I GCGC 2 cut(s) 81, 480
HinP1I GCGC 2 cut(s) 81, 480
HincII GTYRAC 2 cut(s) 30, 36
HindII GTYRAC 2 cut(s) 30, 36
HinfI GANTC 4 cut(s) 325, 457, 473, 518
HpaI GTTAAC 1 cut(s) 36
HpaII CCGG 1 cut(s) 477
Hpy166II GTNNAC 5 cut(s) 30, 36, 132, 226, 472
Hpy188I TCNGA 3 cut(s) 18, 54, 123
Hpy188III TCNNGA 3 cut(s) 188, 268, 293
Hpy8I GTNNAC 5 cut(s) 30, 36, 132, 226, 472
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 242
HpyCH4III ACNGT 2 cut(s) 364, 395
HpyCH4IV ACGT 1 cut(s) 32
HpyCH4V TGCA 3 cut(s) 367, 419, 452
HpyF10VI GCNNNNNNNGC 4 cut(s) 46, 304, 373, 512
HpyF3I CTNAG 3 cut(s) 189, 294, 309
HpySE526I ACGT 1 cut(s) 32
Hsp92II CATG 2 cut(s) 90, 419
HspAI GCGC 2 cut(s) 81, 480
KspAI GTTAAC 1 cut(s) 36
Kzo9I GATC 2 cut(s) 13, 250
LmnI GCTCC 2 cut(s) 295, 442
LpnPI CCDG 8 cut(s) 148, 192, 201, 269, 294, 306, 321, 490
Lsp1109I GCAGC 3 cut(s) 291, 436, 515
MaeII ACGT 1 cut(s) 32
MalI GATC 2 cut(s) 15, 252
MboI GATC 2 cut(s) 13, 250
MboII GAAGA 1 cut(s) 361
MflI RGATCY 1 cut(s) 13
MhlI GDGCHC 2 cut(s) 197, 435
MluCI AATT 2 cut(s) 245, 462
MluI ACGCGT 1 cut(s) 197
MlyI GAGTC 2 cut(s) 319, 467
MmeI TCCRAC 2 cut(s) 174, 182
MnlI CCTC 6 cut(s) 90, 100, 184, 335, 387, 408
MseI TTAA 3 cut(s) 35, 486, 523
MslI CAYNNNNRTG 1 cut(s) 495
MspI CCGG 1 cut(s) 477
MvnI CGCG 4 cut(s) 40, 83, 199, 482
MwoI GCNNNNNNNGC 4 cut(s) 46, 304, 373, 512
NdeII GATC 2 cut(s) 13, 250
NlaIII CATG 2 cut(s) 90, 419
NlaIV GGNNCC 3 cut(s) 15, 69, 167
NspI RCATGY 1 cut(s) 419
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PfeI GAWTC 2 cut(s) 457, 518
PkrI GCNGC 4 cut(s) 85, 306, 451, 505
PleI GAGTC 2 cut(s) 319, 467
PpsI GAGTC 2 cut(s) 319, 467
PspN4I GGNNCC 3 cut(s) 15, 69, 167
PspPI GGNCC 2 cut(s) 68, 384
PstI CTGCAG 1 cut(s) 454
PstNI CAGNNNCTG 2 cut(s) 100, 287
PsuI RGATCY 1 cut(s) 13
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 495
SalI GTCGAC 1 cut(s) 28
SaqAI TTAA 3 cut(s) 35, 486, 523
SatI GCNGC 4 cut(s) 84, 305, 450, 504
Sau3AI GATC 2 cut(s) 13, 250
Sau96I GGNCC 2 cut(s) 68, 384
SchI GAGTC 2 cut(s) 319, 467
SduI GDGCHC 2 cut(s) 197, 435
SetI ASST 7 cut(s) 35, 234, 278, 300, 306, 346, 451
SfcI CTRYAG 1 cut(s) 450
SinI GGWCC 1 cut(s) 68
SmiMI CAYNNNNRTG 1 cut(s) 495
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
Sse9I AATT 2 cut(s) 245, 462
SsiI CCGC 5 cut(s) 47, 65, 83, 177, 515
TaaI ACNGT 2 cut(s) 364, 395
TaiI ACGT 1 cut(s) 35
TaqI TCGA 4 cut(s) 29, 249, 261, 340
TasI AATT 2 cut(s) 245, 462
TatI WGTACW 1 cut(s) 111
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 2 cut(s) 457, 518
Tru1I TTAA 3 cut(s) 35, 486, 523
Tru9I TTAA 3 cut(s) 35, 486, 523
TscAI CASTG 1 cut(s) 292
TseI GCWGC 3 cut(s) 304, 449, 503
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 2 cut(s) 158, 429
TspRI CASTG 1 cut(s) 292
VpaK11BI GGWCC 1 cut(s) 68
XapI RAATTY 1 cut(s) 245
XceI RCATGY 1 cut(s) 419
XmiI GTMKAC 2 cut(s) 29, 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.