MD08G1209200.v1.1

E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0011875
Physical Location & Seq
Reverse (-)
27193938 .. 27195653
1716 bp
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UTR
Exon/CDS
Intron
MD08G1209200.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGAGGGTTGGGACCCGAACACCAAGTCGACGCTGACCCAGATCCCACTCTTGACGACCAAGGCCGGTCCTAGAGACGGCGCGGCTTGGACGCAGAGGCTGAAGGAGGAGTACAAGGCGCTGATCGCCTACACCCAGATGAACAAGTCCAACGACAACGATTGGTTCCGAATCTCAGCCGCCAATCCCGAGGGCACACGATGGACAGGCAAGTGCTGGTACGTCCACAACCTCCTCAAGTACGAATTCGATCTCCAGTTTGATATCCCGATCACCTACCCCGCCACCGCACCGGAACTTGAGCTCCCTCAGCTCGACGGCAAGACCCAAAAGATGTATAGAGGGGGAAAGATCTGCTTGACGGTGCATTTCAAGCCTCTTTGGGCCAAGAACTGCCCAAGATTTGGTATAGCACATGCACTCTGTTTGGGTCTTGCACCGTGGCTTGCAGCAGAGATTCCCATCCTTGTGGATTCTGGTATGGTTAAGCACAAGGATGATGCTGCATCATCGGCTGAATCTTAG

Protein Analysis

175

Amino Acids

19.58

Weight (kDa)

7.66

Isoelectric Point (pI)

29.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFC1 PF08694 8 - 163 2.3e-84 Ubiquitin-fold modifier-conjugating enzyme 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27530
fragaria_vesca FvH4_5g33720 FvH4_5g33720
malus_domestica MD05G1007500.v1.1 MD08G1209200.v1.1 MD15G1393200.v1.1
prunus_persica Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1
pyrus_communis pycom05g10270 pycom08g18060 pycom15g35350
rosa_chinensis RchiOBHm_Chr7g0232851
rosa_laevigata RLG00000001313
rosa_multiflora Rmu_ssc0000018.1_g000017
rosa_roxburghii Rroxscaffold_3G00227850
rosa_rugosa Rorug07G0276400
rosa_samantha Rh7CG450300
rosa_wichuraiana Rw7G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 404
AccI GTMKAC 1 cut(s) 29
AccII CGCG 1 cut(s) 83
AciI CCGC 4 cut(s) 83, 180, 282, 288
AclWI GGATC 1 cut(s) 37
AcsI RAATTY 1 cut(s) 245
AcuI CTGAAG 1 cut(s) 122
AfaI GTAC 3 cut(s) 113, 221, 242
AfiI CCNNNNNNNGG 2 cut(s) 77, 404
AgsI TTSAA 1 cut(s) 373
AloI GAACNNNNNNTCC 2 cut(s) 288, 320
AluBI AGCT 2 cut(s) 304, 313
AluI AGCT 2 cut(s) 304, 313
Alw21I GWGCWC 1 cut(s) 306
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 1 cut(s) 37
AlwNI CAGNNNCTG 1 cut(s) 100
Ama87I CYCGRG 1 cut(s) 188
AoxI GGCC 2 cut(s) 63, 384
ApeKI GCWGC 2 cut(s) 449, 503
ApoI RAATTY 1 cut(s) 245
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AspLEI GCGC 2 cut(s) 83, 121
AspS9I GGNCC 3 cut(s) 13, 68, 384
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 2 cut(s) 13, 68
BaeGI GKGCMC 1 cut(s) 197
BanII GRGCYC 1 cut(s) 306
BarI GAAGNNNNNNTAC 2 cut(s) 95, 127
Bbv12I GWGCWC 1 cut(s) 306
BbvCI CCTCAGC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 461, 490
BccI CCATC 2 cut(s) 195, 470
BceAI ACGGC 2 cut(s) 94, 334
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 1 cut(s) 72
BfoI RGCGCY 1 cut(s) 122
BglII AGATCT 1 cut(s) 351
BisI GCNGC 4 cut(s) 84, 180, 450, 504
BlsI GCNGC 4 cut(s) 85, 181, 451, 505
Bme18I GGWCC 2 cut(s) 13, 68
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 3 cut(s) 13, 68, 384
BmiI GGNNCC 3 cut(s) 14, 15, 167
BmsI GCATC 2 cut(s) 490, 515
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 309
BpuEI CTTGAG 2 cut(s) 221, 320
BsaBI GATNNNNATC 1 cut(s) 461
BsaJI CCNNGG 3 cut(s) 60, 189, 440
BsaWI WCCGGW 1 cut(s) 292
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 404
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 1 cut(s) 256
Bse8I GATNNNNATC 1 cut(s) 461
BseDI CCNNGG 3 cut(s) 60, 189, 440
BseGI GGATG 2 cut(s) 462, 502
BseJI GATNNNNATC 1 cut(s) 461
BseLI CCNNNNNNNGG 2 cut(s) 77, 404
BseMII CTCAG 2 cut(s) 189, 323
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 2 cut(s) 122, 224
BseSI GKGCMC 1 cut(s) 197
BseXI GCAGC 2 cut(s) 461, 490
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 2 cut(s) 65, 386
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 2 cut(s) 66, 293
BslFI GGGAC 1 cut(s) 26
BslI CCNNNNNNNGG 2 cut(s) 77, 404
BsmAI GTCTC 1 cut(s) 69
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 1 cut(s) 26
BsnI GGCC 2 cut(s) 65, 386
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 197, 306
Bsp143I GATC 5 cut(s) 42, 123, 250, 270, 351
BspACI CCGC 4 cut(s) 83, 180, 282, 288
BspANI GGCC 2 cut(s) 65, 386
BspCNI CTCAG 2 cut(s) 188, 322
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 3 cut(s) 14, 15, 167
BspPI GGATC 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 1 cut(s) 256
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 3 cut(s) 60, 189, 440
BssMI GATC 5 cut(s) 42, 123, 250, 270, 351
BssT1I CCWWGG 1 cut(s) 60
Bst4CI ACNGT 2 cut(s) 364, 441
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 3 cut(s) 175, 309, 522
BstDSI CCRYGG 1 cut(s) 440
BstF5I GGATG 2 cut(s) 462, 502
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 2 cut(s) 83, 121
BstKTI GATC 5 cut(s) 45, 126, 253, 273, 354
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 5 cut(s) 42, 123, 250, 270, 351
BstMWI GCNNNNNNNGC 4 cut(s) 125, 310, 373, 512
BstNSI RCATGY 1 cut(s) 419
BstSLI GKGCMC 1 cut(s) 197
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 2 cut(s) 461, 490
BstX2I RGATCY 2 cut(s) 42, 351
BstXI CCANNNNNNTGG 1 cut(s) 469
BstYI RGATCY 2 cut(s) 42, 351
BsuRI GGCC 2 cut(s) 65, 386
BtgI CCRYGG 1 cut(s) 440
BtsCI GGATG 2 cut(s) 462, 502
Cac8I GCNNGC 1 cut(s) 447
CaiI CAGNNNCTG 1 cut(s) 100
CfoI GCGC 2 cut(s) 83, 121
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 13, 68, 384
CseI GACGC 2 cut(s) 40, 100
Csp6I GTAC 3 cut(s) 112, 220, 241
CviAII CATG 1 cut(s) 416
CviQI GTAC 3 cut(s) 112, 220, 241
DdeI CTNAG 3 cut(s) 175, 309, 522
DpnI GATC 5 cut(s) 44, 125, 252, 272, 353
DpnII GATC 5 cut(s) 42, 123, 250, 270, 351
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 60
Eco24I GRGCYC 1 cut(s) 306
Eco32I GATATC 1 cut(s) 265
Eco47I GGWCC 2 cut(s) 13, 68
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 13
EcoRI GAATTC 1 cut(s) 245
EcoRV GATATC 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 60
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 60
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 1 cut(s) 419
FaiI YATR 4 cut(s) 339, 410, 417, 482
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 1 cut(s) 26
FatI CATG 1 cut(s) 415
FauI CCCGC 1 cut(s) 289
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 4 cut(s) 84, 180, 450, 504
FokI GGATG 2 cut(s) 449, 509
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 4 cut(s) 84, 180, 450, 504
FspBI CTAG 1 cut(s) 72
GlaI GCGC 2 cut(s) 82, 120
GluI GCNGC 4 cut(s) 84, 180, 450, 504
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 2 cut(s) 65, 386
HapII CCGG 2 cut(s) 66, 293
HgaI GACGC 2 cut(s) 40, 100
HhaI GCGC 2 cut(s) 83, 121
Hin1II CATG 1 cut(s) 419
Hin6I GCGC 2 cut(s) 81, 119
HinP1I GCGC 2 cut(s) 81, 119
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 4 cut(s) 171, 457, 473, 518
HpaII CCGG 2 cut(s) 66, 293
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 2 cut(s) 30, 226
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 3 cut(s) 52, 188, 268
Hpy8I GTNNAC 2 cut(s) 30, 226
Hpy99I CGWCG 2 cut(s) 34, 320
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 2 cut(s) 364, 441
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 5 cut(s) 367, 419, 437, 449, 506
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 310, 373, 512
HpyF3I CTNAG 3 cut(s) 175, 309, 522
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 1 cut(s) 419
HspAI GCGC 2 cut(s) 81, 119
KflI GGGWCCC 1 cut(s) 13
Kzo9I GATC 5 cut(s) 42, 123, 250, 270, 351
LmnI GCTCC 1 cut(s) 309
LpnPI CCDG 8 cut(s) 53, 79, 149, 192, 202, 269, 306, 462
Lsp1109I GCAGC 2 cut(s) 461, 490
LweI GCATC 2 cut(s) 490, 515
MaeI CTAG 1 cut(s) 72
MaeII ACGT 1 cut(s) 222
MalI GATC 5 cut(s) 44, 125, 252, 272, 353
MboI GATC 5 cut(s) 42, 123, 250, 270, 351
MflI RGATCY 2 cut(s) 42, 351
MhlI GDGCHC 2 cut(s) 197, 306
MluCI AATT 1 cut(s) 245
MmeI TCCRAC 1 cut(s) 174
MnlI CCTC 8 cut(s) 90, 100, 184, 242, 245, 318, 335, 387
MseI TTAA 1 cut(s) 486
MslI CAYNNNNRTG 3 cut(s) 137, 467, 495
MspI CCGG 2 cut(s) 66, 293
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 4 cut(s) 125, 310, 373, 512
NdeII GATC 5 cut(s) 42, 123, 250, 270, 351
NlaIII CATG 1 cut(s) 419
NlaIV GGNNCC 3 cut(s) 14, 15, 167
NspI RCATGY 1 cut(s) 419
PfeI GAWTC 4 cut(s) 171, 457, 473, 518
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 4 cut(s) 85, 181, 451, 505
PpuMI RGGWCCY 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 13
PspN4I GGNNCC 3 cut(s) 14, 15, 167
PspPI GGNCC 3 cut(s) 13, 68, 384
PspPPI RGGWCCY 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 100
PsuI RGATCY 2 cut(s) 42, 351
RsaI GTAC 3 cut(s) 113, 221, 242
RsaNI GTAC 3 cut(s) 112, 220, 241
RseI CAYNNNNRTG 3 cut(s) 137, 467, 495
SacI GAGCTC 1 cut(s) 306
SalI GTCGAC 1 cut(s) 28
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 4 cut(s) 84, 180, 450, 504
Sau3AI GATC 5 cut(s) 42, 123, 250, 270, 351
Sau96I GGNCC 3 cut(s) 13, 68, 384
SduI GDGCHC 2 cut(s) 197, 306
SetI ASST 5 cut(s) 225, 234, 278, 306, 315
SfaNI GCATC 2 cut(s) 490, 515
SinI GGWCC 2 cut(s) 13, 68
SmiMI CAYNNNNRTG 3 cut(s) 137, 467, 495
SmlI CTYRAG 2 cut(s) 236, 299
SmoI CTYRAG 2 cut(s) 236, 299
Sse9I AATT 1 cut(s) 245
SsiI CCGC 4 cut(s) 83, 180, 282, 288
SspMI CTAG 1 cut(s) 72
SstI GAGCTC 1 cut(s) 306
StyI CCWWGG 1 cut(s) 60
TaaI ACNGT 2 cut(s) 364, 441
TaiI ACGT 1 cut(s) 225
TaqI TCGA 3 cut(s) 29, 249, 315
TasI AATT 1 cut(s) 245
TatI WGTACW 1 cut(s) 111
TauI GCSGC 2 cut(s) 86, 182
TfiI GAWTC 4 cut(s) 171, 457, 473, 518
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TseI GCWGC 2 cut(s) 449, 503
TspDTI ATGAA 1 cut(s) 155
Van91I CCANNNNNTGG 1 cut(s) 404
VpaK11BI GGWCC 2 cut(s) 13, 68
XapI RAATTY 1 cut(s) 245
XceI RCATGY 1 cut(s) 419
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.