pycom08g18060

E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0011875
Physical Location & Seq
Reverse (-)
18277073 .. 18278255
1183 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g18060.1

Sequence Viewer

Length: 387 bp
ATGAACAAGTCCAACGACAACGATTGGTTCCGAATCTCCGCCGCCAATCCCGAGGGCACACGGTGGACGGGCAAGTGCTGGTACATCCACAACCTCCTCAAGCACGAATTCGATCTCCAGTTTGATATCCCGATCACCTACCCCGCCACTGCACCGGAACTTGAGCTCCCTCAGCTCGACGGCAAGACCCAAAAGATGTATAGAGGGGGAAAGATCTGCTTGACGGTGCATTTCAAGCCTCTTTGGGCCAAGAACTGCCCAAGATTTGGTATAGCACATGCACTCTGTTTGGGTCTTGCACCATGGCTTGCAGCAGAGATTCCCATCCTTGTGGATTCTGGTATGGTTAAGCACAAAGATGATGCTGCATCATCAGCTGAATCTTAG

Protein Analysis

129

Amino Acids

14.39

Weight (kDa)

6.95

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFC1 PF08694 1 - 117 2e-64 Ubiquitin-fold modifier-conjugating enzyme 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27530
fragaria_vesca FvH4_5g33720 FvH4_5g33720
malus_domestica MD05G1007500.v1.1 MD08G1209200.v1.1 MD15G1393200.v1.1
prunus_persica Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1
pyrus_communis pycom05g10270 pycom08g18060 pycom15g35350
rosa_chinensis RchiOBHm_Chr7g0232851
rosa_laevigata RLG00000001313
rosa_multiflora Rmu_ssc0000018.1_g000017
rosa_roxburghii Rroxscaffold_3G00227850
rosa_rugosa Rorug07G0276400
rosa_samantha Rh7CG450300
rosa_wichuraiana Rw7G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 266
AciI CCGC 3 cut(s) 39, 42, 144
AcsI RAATTY 1 cut(s) 107
AdeI CACNNNGTG 1 cut(s) 63
AfaI GTAC 1 cut(s) 83
AfiI CCNNNNNNNGG 1 cut(s) 266
AgsI TTSAA 1 cut(s) 235
AloI GAACNNNNNNTCC 2 cut(s) 150, 182
AluBI AGCT 3 cut(s) 166, 175, 377
AluI AGCT 3 cut(s) 166, 175, 377
Alw21I GWGCWC 1 cut(s) 168
Ama87I CYCGRG 1 cut(s) 50
AoxI GGCC 1 cut(s) 246
ApeKI GCWGC 2 cut(s) 311, 365
ApoI RAATTY 1 cut(s) 107
AspS9I GGNCC 1 cut(s) 246
AsuHPI GGTGA 1 cut(s) 127
AvaI CYCGRG 1 cut(s) 50
BaeGI GKGCMC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 168
BbvCI CCTCAGC 1 cut(s) 171
BbvI GCAGC 2 cut(s) 323, 352
BccI CCATC 1 cut(s) 332
BceAI ACGGC 1 cut(s) 196
BglII AGATCT 1 cut(s) 213
BisI GCNGC 3 cut(s) 42, 312, 366
BlsI GCNGC 3 cut(s) 43, 313, 367
BmeT110I CYCGRG 1 cut(s) 50
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 1 cut(s) 29
BmsI GCATC 2 cut(s) 352, 377
BpmI CTGGAG 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 171
BpuEI CTTGAG 2 cut(s) 83, 182
BsaBI GATNNNNATC 1 cut(s) 323
BsaJI CCNNGG 2 cut(s) 51, 302
BsaWI WCCGGW 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 1 cut(s) 266
Bse1I ACTGG 1 cut(s) 118
Bse8I GATNNNNATC 1 cut(s) 323
BseDI CCNNGG 2 cut(s) 51, 302
BseGI GGATG 2 cut(s) 84, 324
BseJI GATNNNNATC 1 cut(s) 323
BseLI CCNNNNNNNGG 1 cut(s) 266
BseMII CTCAG 1 cut(s) 185
BseNI ACTGG 1 cut(s) 118
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 59
BseXI GCAGC 2 cut(s) 323, 352
BsgI GTGCAG 1 cut(s) 135
BshFI GGCC 1 cut(s) 248
BsiHKAI GWGCWC 1 cut(s) 168
BsiHKCI CYCGRG 1 cut(s) 50
BsiSI CCGG 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 266
BsnI GGCC 1 cut(s) 248
BsoBI CYCGRG 1 cut(s) 50
Bsp1286I GDGCHC 2 cut(s) 59, 168
Bsp143I GATC 3 cut(s) 112, 132, 213
Bsp19I CCATGG 1 cut(s) 302
BspACI CCGC 3 cut(s) 39, 42, 144
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 1 cut(s) 184
BspLI GGNNCC 1 cut(s) 29
BsrI ACTGG 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 51, 302
BssMI GATC 3 cut(s) 112, 132, 213
BssT1I CCWWGG 1 cut(s) 302
Bst4CI ACNGT 2 cut(s) 63, 226
BstC8I GCNNGC 1 cut(s) 309
BstDEI CTNAG 2 cut(s) 171, 384
BstDSI CCRYGG 1 cut(s) 302
BstF5I GGATG 2 cut(s) 84, 324
BstKTI GATC 3 cut(s) 115, 135, 216
BstMBI GATC 3 cut(s) 112, 132, 213
BstMWI GCNNNNNNNGC 3 cut(s) 172, 235, 374
BstNSI RCATGY 1 cut(s) 281
BstSLI GKGCMC 1 cut(s) 59
BstV1I GCAGC 2 cut(s) 323, 352
BstX2I RGATCY 1 cut(s) 213
BstXI CCANNNNNNTGG 1 cut(s) 331
BstYI RGATCY 1 cut(s) 213
BsuRI GGCC 1 cut(s) 248
BtgI CCRYGG 1 cut(s) 302
BtsCI GGATG 2 cut(s) 84, 324
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 246
Csp6I GTAC 1 cut(s) 82
CviAII CATG 2 cut(s) 278, 303
CviJI RGCY 6 cut(s) 166, 175, 238, 248, 307, 377
CviKI_1 RGCY 6 cut(s) 166, 175, 238, 248, 307, 377
CviQI GTAC 1 cut(s) 82
DdeI CTNAG 2 cut(s) 171, 384
DpnI GATC 3 cut(s) 114, 134, 215
DpnII GATC 3 cut(s) 112, 132, 213
DraIII CACNNNGTG 1 cut(s) 63
EciI GGCGGA 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 166
Eco130I CCWWGG 1 cut(s) 302
Eco24I GRGCYC 1 cut(s) 168
Eco32I GATATC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 166
Eco88I CYCGRG 1 cut(s) 50
EcoICRI GAGCTC 1 cut(s) 166
EcoRI GAATTC 1 cut(s) 107
EcoRV GATATC 1 cut(s) 127
EcoT14I CCWWGG 1 cut(s) 302
EcoT38I GRGCYC 1 cut(s) 168
ErhI CCWWGG 1 cut(s) 302
FaeI CATG 2 cut(s) 281, 306
FaiI YATR 5 cut(s) 201, 272, 279, 304, 344
FalI AAGNNNNNCTT 2 cut(s) 203, 235
FatI CATG 2 cut(s) 277, 302
FauI CCCGC 1 cut(s) 151
Fnu4HI GCNGC 3 cut(s) 42, 312, 366
FokI GGATG 2 cut(s) 71, 311
FriOI GRGCYC 1 cut(s) 168
Fsp4HI GCNGC 3 cut(s) 42, 312, 366
GluI GCNGC 3 cut(s) 42, 312, 366
GsuI CTGGAG 1 cut(s) 101
HaeIII GGCC 1 cut(s) 248
HapII CCGG 1 cut(s) 155
Hin1II CATG 2 cut(s) 281, 306
HinfI GANTC 4 cut(s) 33, 319, 335, 380
HpaII CCGG 1 cut(s) 155
HphI GGTGA 1 cut(s) 127
Hpy166II GTNNAC 1 cut(s) 66
Hpy188I TCNGA 1 cut(s) 32
Hpy188III TCNNGA 2 cut(s) 50, 130
Hpy8I GTNNAC 1 cut(s) 66
Hpy99I CGWCG 1 cut(s) 182
HpyCH4III ACNGT 2 cut(s) 63, 226
HpyCH4V TGCA 6 cut(s) 152, 229, 281, 299, 311, 368
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 235, 374
HpyF3I CTNAG 2 cut(s) 171, 384
Hsp92II CATG 2 cut(s) 281, 306
Kzo9I GATC 3 cut(s) 112, 132, 213
LmnI GCTCC 1 cut(s) 171
LpnPI CCDG 4 cut(s) 64, 131, 168, 324
Lsp1109I GCAGC 2 cut(s) 323, 352
LweI GCATC 2 cut(s) 352, 377
MalI GATC 3 cut(s) 114, 134, 215
MboI GATC 3 cut(s) 112, 132, 213
MflI RGATCY 1 cut(s) 213
MhlI GDGCHC 2 cut(s) 59, 168
MluCI AATT 1 cut(s) 107
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 6 cut(s) 46, 104, 107, 180, 197, 249
MseI TTAA 1 cut(s) 348
MslI CAYNNNNRTG 2 cut(s) 329, 357
MspA1I CMGCKG 1 cut(s) 377
MspI CCGG 1 cut(s) 155
MwoI GCNNNNNNNGC 3 cut(s) 172, 235, 374
NcoI CCATGG 1 cut(s) 302
NdeII GATC 3 cut(s) 112, 132, 213
NlaIII CATG 2 cut(s) 281, 306
NlaIV GGNNCC 1 cut(s) 29
NspI RCATGY 1 cut(s) 281
PfeI GAWTC 4 cut(s) 33, 319, 335, 380
PflMI CCANNNNNTGG 1 cut(s) 266
PkrI GCNGC 3 cut(s) 43, 313, 367
Psp124BI GAGCTC 1 cut(s) 168
PspN4I GGNNCC 1 cut(s) 29
PspPI GGNCC 1 cut(s) 246
PsuI RGATCY 1 cut(s) 213
PvuII CAGCTG 1 cut(s) 377
RsaI GTAC 1 cut(s) 83
RsaNI GTAC 1 cut(s) 82
RseI CAYNNNNRTG 2 cut(s) 329, 357
SacI GAGCTC 1 cut(s) 168
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 3 cut(s) 42, 312, 366
Sau3AI GATC 3 cut(s) 112, 132, 213
Sau96I GGNCC 1 cut(s) 246
SduI GDGCHC 2 cut(s) 59, 168
SetI ASST 5 cut(s) 96, 140, 168, 177, 379
SfaNI GCATC 2 cut(s) 352, 377
SmiMI CAYNNNNRTG 2 cut(s) 329, 357
SmlI CTYRAG 2 cut(s) 98, 161
SmoI CTYRAG 2 cut(s) 98, 161
Sse9I AATT 1 cut(s) 107
SsiI CCGC 3 cut(s) 39, 42, 144
SstI GAGCTC 1 cut(s) 168
StyI CCWWGG 1 cut(s) 302
TaaI ACNGT 2 cut(s) 63, 226
TaqI TCGA 2 cut(s) 111, 177
TasI AATT 1 cut(s) 107
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 4 cut(s) 33, 319, 335, 380
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 1 cut(s) 154
TseI GCWGC 2 cut(s) 311, 365
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 154
Van91I CCANNNNNTGG 1 cut(s) 266
XapI RAATTY 1 cut(s) 107
XceI RCATGY 1 cut(s) 281
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.