Rw7G035630

E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0011875
Physical Location & Seq
Forward (+)
53403159 .. 53407157
3999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G035630.1

Sequence Viewer

Length: 516 bp
ATGGAGGGTTGGGACCCGAACACGAAGTCGACGCTGACCCAGATCCCTCTCCTGACCACCAAGGCCGGGCCGCGAGATGGGCCGGCGTGGACCCAGAGGCTGAAAGAGGAGTACAAGTCGCTGATCGCCTACACCCAGATGAACAAGTCCAACGACAACGATTGGTTCCGAATCTCCGCCGCCAATCCCGAAGGGACACGTTGGACCGGCAAGTGCTGGTACGTCCACAACCTCCTCAAGTACGAGTTCGACCTCCAGTTCGATATCCCGGTCACTTACCCTGCCACCGCGCCGGAGCTCGAGCTCCCCAGCTCGATGGGAAAACCCAAAAGAGGAGGGAAGATTTGCCTGACCGTGCATTTCAAGCCGCTTTGGGCCAAAAACTGCCCTAGATTTGGTATAGCGCATGCTCTCTGTTTGGGTCTTGCTCCATGGCTTGCAGCAGAGATACCCATTCTTGTTGATTCTGGTATGGTCAAGCACAAAGATGATGCAGCAACATCGGCTGAATCTTAG

Protein Analysis

171

Amino Acids

19.15

Weight (kDa)

8.31

Isoelectric Point (pI)

32.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFC1 PF08694 8 - 160 3.3e-75 Ubiquitin-fold modifier-conjugating enzyme 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27530
fragaria_vesca FvH4_5g33720 FvH4_5g33720
malus_domestica MD05G1007500.v1.1 MD08G1209200.v1.1 MD15G1393200.v1.1
prunus_persica Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1
pyrus_communis pycom05g10270 pycom08g18060 pycom15g35350
rosa_chinensis RchiOBHm_Chr7g0232851
rosa_laevigata RLG00000001313
rosa_multiflora Rmu_ssc0000018.1_g000017
rosa_roxburghii Rroxscaffold_3G00227850
rosa_rugosa Rorug07G0276400
rosa_samantha Rh7CG450300
rosa_wichuraiana Rw7G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 29
AccII CGCG 2 cut(s) 73, 290
AciI CCGC 5 cut(s) 71, 177, 180, 288, 368
AclWI GGATC 1 cut(s) 37
AfaI GTAC 3 cut(s) 113, 221, 242
AfiI CCNNNNNNNGG 4 cut(s) 66, 77, 332, 395
AflIII ACRYGT 1 cut(s) 197
AgsI TTSAA 1 cut(s) 364
AluBI AGCT 3 cut(s) 298, 304, 312
AluI AGCT 3 cut(s) 298, 304, 312
Alw21I GWGCWC 2 cut(s) 300, 306
AlwI GGATC 1 cut(s) 37
AlwNI CAGNNNCTG 1 cut(s) 100
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 4 cut(s) 63, 68, 80, 375
ApeKI GCWGC 2 cut(s) 440, 494
ArsI GACNNNNNNTTYG 4 cut(s) 11, 43, 242, 274
AspLEI GCGC 2 cut(s) 292, 406
AspS9I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 375
AsuC2I CCSGG 2 cut(s) 67, 269
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 3 cut(s) 13, 90, 204
BanII GRGCYC 2 cut(s) 300, 306
Bbv12I GWGCWC 2 cut(s) 300, 306
BbvI GCAGC 2 cut(s) 452, 506
BccI CCATC 2 cut(s) 71, 310
BcgI CGANNNNNNTGC 1 cut(s) 483
BcnI CCSGG 2 cut(s) 67, 269
BfaI CTAG 1 cut(s) 390
BisI GCNGC 5 cut(s) 71, 180, 368, 441, 495
BlsI GCNGC 5 cut(s) 72, 181, 369, 442, 496
Bme1390I CCNGG 2 cut(s) 67, 269
Bme18I GGWCC 3 cut(s) 13, 90, 204
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 375
BmiI GGNNCC 4 cut(s) 14, 15, 92, 167
BmrFI CCNGG 2 cut(s) 67, 269
BmsI GCATC 1 cut(s) 481
BpmI CTGGAG 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 2 cut(s) 67, 269
BsaJI CCNNGG 2 cut(s) 60, 431
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 4 cut(s) 66, 77, 332, 395
Bse118I RCCGGY 2 cut(s) 82, 206
Bse1I ACTGG 1 cut(s) 256
BseDI CCNNGG 2 cut(s) 60, 431
BseLI CCNNNNNNNGG 4 cut(s) 66, 77, 332, 395
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 3 cut(s) 122, 224, 348
BseXI GCAGC 2 cut(s) 452, 506
BseYI CCCAGC 1 cut(s) 308
Bsh1236I CGCG 2 cut(s) 73, 290
BshFI GGCC 4 cut(s) 65, 70, 82, 377
BsiHKAI GWGCWC 2 cut(s) 300, 306
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 5 cut(s) 66, 83, 207, 269, 293
BslFI GGGAC 2 cut(s) 26, 208
BslI CCNNNNNNNGG 4 cut(s) 66, 77, 332, 395
BsmFI GGGAC 2 cut(s) 26, 208
BsnI GGCC 4 cut(s) 65, 70, 82, 377
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 300, 306
Bsp143I GATC 2 cut(s) 42, 123
Bsp19I CCATGG 1 cut(s) 431
BspACI CCGC 5 cut(s) 71, 177, 180, 288, 368
BspANI GGCC 4 cut(s) 65, 70, 82, 377
BspFNI CGCG 2 cut(s) 73, 290
BspLI GGNNCC 4 cut(s) 14, 15, 92, 167
BspPI GGATC 1 cut(s) 37
BsrFI RCCGGY 2 cut(s) 82, 206
BsrI ACTGG 1 cut(s) 256
BssAI RCCGGY 2 cut(s) 82, 206
BssECI CCNNGG 2 cut(s) 60, 431
BssMI GATC 2 cut(s) 42, 123
BssT1I CCWWGG 2 cut(s) 60, 431
Bst4CI ACNGT 1 cut(s) 355
BstC8I GCNNGC 3 cut(s) 84, 408, 438
BstDEI CTNAG 1 cut(s) 513
BstDSI CCRYGG 1 cut(s) 431
BstFNI CGCG 2 cut(s) 73, 290
BstHHI GCGC 2 cut(s) 292, 406
BstKTI GATC 2 cut(s) 45, 126
BstMBI GATC 2 cut(s) 42, 123
BstMWI GCNNNNNNNGC 3 cut(s) 79, 364, 503
BstNSI RCATGY 1 cut(s) 410
BstSCI CCNGG 2 cut(s) 65, 267
BstUI CGCG 2 cut(s) 73, 290
BstV1I GCAGC 2 cut(s) 452, 506
BstX2I RGATCY 1 cut(s) 42
BstXI CCANNNNNNTGG 1 cut(s) 316
BstYI RGATCY 1 cut(s) 42
BsuRI GGCC 4 cut(s) 65, 70, 82, 377
BtgI CCRYGG 1 cut(s) 431
Cac8I GCNNGC 3 cut(s) 84, 408, 438
CaiI CAGNNNCTG 1 cut(s) 100
CfoI GCGC 2 cut(s) 292, 406
Cfr10I RCCGGY 2 cut(s) 82, 206
Cfr13I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 375
CseI GACGC 1 cut(s) 40
Csp6I GTAC 3 cut(s) 112, 220, 241
CviAII CATG 2 cut(s) 407, 432
CviQI GTAC 3 cut(s) 112, 220, 241
DdeI CTNAG 1 cut(s) 513
DpnI GATC 2 cut(s) 44, 125
DpnII GATC 2 cut(s) 42, 123
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 2 cut(s) 298, 304
Eco130I CCWWGG 2 cut(s) 60, 431
Eco24I GRGCYC 2 cut(s) 300, 306
Eco32I GATATC 1 cut(s) 265
Eco47I GGWCC 3 cut(s) 13, 90, 204
Eco53kI GAGCTC 2 cut(s) 298, 304
Eco88I CYCGRG 1 cut(s) 299
EcoICRI GAGCTC 2 cut(s) 298, 304
EcoO109I RGGNCCY 1 cut(s) 13
EcoRV GATATC 1 cut(s) 265
EcoT14I CCWWGG 2 cut(s) 60, 431
EcoT38I GRGCYC 2 cut(s) 300, 306
ErhI CCWWGG 2 cut(s) 60, 431
FaeI CATG 2 cut(s) 410, 435
FaiI YATR 4 cut(s) 401, 408, 433, 473
FaqI GGGAC 2 cut(s) 26, 208
FatI CATG 2 cut(s) 406, 431
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 5 cut(s) 71, 180, 368, 441, 495
FriOI GRGCYC 2 cut(s) 300, 306
Fsp4HI GCNGC 5 cut(s) 71, 180, 368, 441, 495
FspBI CTAG 1 cut(s) 390
GlaI GCGC 2 cut(s) 291, 405
GluI GCNGC 5 cut(s) 71, 180, 368, 441, 495
GsaI CCCAGC 1 cut(s) 312
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 4 cut(s) 65, 70, 82, 377
HapII CCGG 5 cut(s) 66, 83, 207, 269, 293
HgaI GACGC 1 cut(s) 40
HhaI GCGC 2 cut(s) 292, 406
Hin1II CATG 2 cut(s) 410, 435
Hin6I GCGC 2 cut(s) 290, 404
HinP1I GCGC 2 cut(s) 290, 404
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 3 cut(s) 171, 464, 509
HpaII CCGG 5 cut(s) 66, 83, 207, 269, 293
Hpy166II GTNNAC 3 cut(s) 30, 90, 226
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 2 cut(s) 52, 188
Hpy8I GTNNAC 3 cut(s) 30, 90, 226
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 2 cut(s) 199, 222
HpyCH4V TGCA 3 cut(s) 358, 440, 494
HpyF10VI GCNNNNNNNGC 3 cut(s) 79, 364, 503
HpyF3I CTNAG 1 cut(s) 513
HpySE526I ACGT 2 cut(s) 199, 222
Hsp92II CATG 2 cut(s) 410, 435
HspAI GCGC 2 cut(s) 290, 404
KflI GGGWCCC 1 cut(s) 13
KroI GCCGGC 1 cut(s) 82
KroNI GCCGGC 1 cut(s) 84
Kzo9I GATC 2 cut(s) 42, 123
LmnI GCTCC 3 cut(s) 295, 309, 433
Lsp1109I GCAGC 2 cut(s) 452, 506
LweI GCATC 1 cut(s) 481
MaeI CTAG 1 cut(s) 390
MaeII ACGT 2 cut(s) 199, 222
MaeIII GTNAC 1 cut(s) 271
MalI GATC 2 cut(s) 44, 125
MboI GATC 2 cut(s) 42, 123
MboII GAAGA 1 cut(s) 352
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 2 cut(s) 300, 306
MmeI TCCRAC 2 cut(s) 174, 182
MnlI CCTC 8 cut(s) 57, 90, 100, 242, 245, 263, 326, 329
MroNI GCCGGC 1 cut(s) 82
MslI CAYNNNNRTG 2 cut(s) 137, 486
MspI CCGG 5 cut(s) 66, 83, 207, 269, 293
MspR9I CCNGG 2 cut(s) 67, 269
MvnI CGCG 2 cut(s) 73, 290
MwoI GCNNNNNNNGC 3 cut(s) 79, 364, 503
NaeI GCCGGC 1 cut(s) 84
NciI CCSGG 2 cut(s) 67, 269
NcoI CCATGG 1 cut(s) 431
NdeII GATC 2 cut(s) 42, 123
NgoMIV GCCGGC 1 cut(s) 82
NlaIII CATG 2 cut(s) 410, 435
NlaIV GGNNCC 4 cut(s) 14, 15, 92, 167
NmuCI GTSAC 1 cut(s) 271
NspI RCATGY 1 cut(s) 410
PaeI GCATGC 1 cut(s) 410
PaeR7I CTCGAG 1 cut(s) 299
PdiI GCCGGC 1 cut(s) 84
PfeI GAWTC 3 cut(s) 171, 464, 509
PkrI GCNGC 5 cut(s) 72, 181, 369, 442, 496
PpuMI RGGWCCY 1 cut(s) 13
Psp124BI GAGCTC 2 cut(s) 300, 306
Psp5II RGGWCCY 1 cut(s) 13
PspFI CCCAGC 1 cut(s) 308
PspN4I GGNNCC 4 cut(s) 14, 15, 92, 167
PspPI GGNCC 6 cut(s) 13, 68, 80, 90, 204, 375
PspPPI RGGWCCY 1 cut(s) 13
PspXI VCTCGAGB 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 100
PsuI RGATCY 1 cut(s) 42
RsaI GTAC 3 cut(s) 113, 221, 242
RsaNI GTAC 3 cut(s) 112, 220, 241
RseI CAYNNNNRTG 2 cut(s) 137, 486
SacI GAGCTC 2 cut(s) 300, 306
SalI GTCGAC 1 cut(s) 28
SatI GCNGC 5 cut(s) 71, 180, 368, 441, 495
Sau3AI GATC 2 cut(s) 42, 123
Sau96I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 375
ScrFI CCNGG 2 cut(s) 67, 269
SduI GDGCHC 2 cut(s) 300, 306
SetI ASST 7 cut(s) 202, 225, 234, 255, 300, 306, 314
SfaNI GCATC 1 cut(s) 481
Sfr274I CTCGAG 1 cut(s) 299
SinI GGWCC 3 cut(s) 13, 90, 204
SlaI CTCGAG 1 cut(s) 299
SmiMI CAYNNNNRTG 2 cut(s) 137, 486
SmlI CTYRAG 2 cut(s) 236, 299
SmoI CTYRAG 2 cut(s) 236, 299
SphI GCATGC 1 cut(s) 410
SsiI CCGC 5 cut(s) 71, 177, 180, 288, 368
SspMI CTAG 1 cut(s) 390
SstI GAGCTC 2 cut(s) 300, 306
StyD4I CCNGG 2 cut(s) 65, 267
StyI CCWWGG 2 cut(s) 60, 431
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 2 cut(s) 202, 225
TaqI TCGA 5 cut(s) 29, 249, 261, 300, 314
TatI WGTACW 1 cut(s) 111
TauI GCSGC 3 cut(s) 73, 182, 370
TfiI GAWTC 3 cut(s) 171, 464, 509
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 2 cut(s) 440, 494
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 1 cut(s) 155
VpaK11BI GGWCC 3 cut(s) 13, 90, 204
XceI RCATGY 1 cut(s) 410
XhoI CTCGAG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.