FvH4_5g33720

E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0011875
Physical Location & Seq
Forward (+)
24424657 .. 24427376
2720 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33720.t2

Sequence Viewer

Length: 525 bp
ATGGAGGGTTGGGACCCGAACACGAAGTCGACGCTGACCCAGATCCCGCTCCTGACCACCAAGGCCGGACCCCGAGACGGACCGGCGTGGACCCAGCGGCTCAAAGAGGAGTACAAGTCGCTGATCGCCTACACCCAGATGAACAAGTCCAACGACAACGATTGGTTCCGAATCTCCGCCGCCAATCCCGAAGGGACACGTTGGACCGGCACGTGCTGGTACGTCCACAACCTCCTCAGGTATGAGTTCGACCTCCAGTTCGATATTCCCGTCACCTACCCCGCCACCGCGCCTGAGCTCGAGCTCCCCCAGCTCGATGGAAAAACCCAAAAGATGTATAGAGGAGGGAAGATTTGCTTGACCGTGCATTTCAAGCCGCTCTGGGCCAAAAACTGCCCTAGATTTGGTATAGCACATGCTCTCTGTTTGGGTCTTGCTCCATGGCTTGCAGCAGAGATTCCCATTCTTGTGGATTCTGGTATGGTCAAGCACAAAGATGATGCAGCAACATCGGCCGAATCTTAG

Protein Analysis

175

Amino Acids

19.63

Weight (kDa)

6.89

Isoelectric Point (pI)

32.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFC1 PF08694 8 - 112 3.3e-48 Ubiquitin-fold modifier-conjugating enzyme 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27530
fragaria_vesca FvH4_5g33720 FvH4_5g33720
malus_domestica MD05G1007500.v1.1 MD08G1209200.v1.1 MD15G1393200.v1.1
prunus_persica Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1
pyrus_communis pycom05g10270 pycom08g18060 pycom15g35350
rosa_chinensis RchiOBHm_Chr7g0232851
rosa_laevigata RLG00000001313
rosa_multiflora Rmu_ssc0000018.1_g000017
rosa_roxburghii Rroxscaffold_3G00227850
rosa_rugosa Rorug07G0276400
rosa_samantha Rh7CG450300
rosa_wichuraiana Rw7G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 49, 379
AccI GTMKAC 1 cut(s) 29
AccII CGCG 1 cut(s) 290
AciI CCGC 7 cut(s) 47, 97, 177, 180, 282, 288, 377
AclWI GGATC 1 cut(s) 37
AcoI YGGCCR 1 cut(s) 513
AcvI CACGTG 1 cut(s) 213
AfaI GTAC 2 cut(s) 113, 221
AfiI CCNNNNNNNGG 2 cut(s) 77, 404
AflIII ACRYGT 1 cut(s) 197
AgsI TTSAA 1 cut(s) 373
AluBI AGCT 3 cut(s) 298, 304, 313
AluI AGCT 3 cut(s) 298, 304, 313
Alw21I GWGCWC 2 cut(s) 300, 306
Alw26I GTCTC 1 cut(s) 69
AlwI GGATC 1 cut(s) 37
Ama87I CYCGRG 2 cut(s) 72, 299
AoxI GGCC 3 cut(s) 63, 384, 513
ApeKI GCWGC 2 cut(s) 449, 503
ArsI GACNNNNNNTTYG 4 cut(s) 11, 43, 242, 274
AspLEI GCGC 1 cut(s) 292
AspS9I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 384
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 2 cut(s) 72, 299
AvaII GGWCC 5 cut(s) 13, 68, 80, 90, 204
AxyI CCTNAGG 1 cut(s) 236
BanII GRGCYC 2 cut(s) 300, 306
BbrPI CACGTG 1 cut(s) 213
Bbv12I GWGCWC 2 cut(s) 300, 306
BbvI GCAGC 2 cut(s) 461, 515
BccI CCATC 1 cut(s) 311
BcgI CGANNNNNNTGC 1 cut(s) 492
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 1 cut(s) 399
BisI GCNGC 5 cut(s) 98, 180, 377, 450, 504
BlsI GCNGC 5 cut(s) 99, 181, 378, 451, 505
Bme18I GGWCC 5 cut(s) 13, 68, 80, 90, 204
BmeT110I CYCGRG 2 cut(s) 72, 299
BmgT120I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 384
BmiI GGNNCC 5 cut(s) 14, 15, 70, 92, 167
BmsI GCATC 1 cut(s) 490
BpmI CTGGAG 1 cut(s) 239
Bpu10I CCTNAGC 1 cut(s) 294
BsaAI YACGTR 1 cut(s) 213
BsaJI CCNNGG 2 cut(s) 60, 440
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 404
Bse118I RCCGGY 2 cut(s) 82, 206
Bse1I ACTGG 1 cut(s) 256
Bse21I CCTNAGG 1 cut(s) 236
BseDI CCNNGG 2 cut(s) 60, 440
BseLI CCNNNNNNNGG 2 cut(s) 77, 404
BseMII CTCAG 2 cut(s) 250, 285
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 3 cut(s) 122, 224, 357
BseX3I CGGCCG 1 cut(s) 513
BseXI GCAGC 2 cut(s) 461, 515
BseYI CCCAGC 2 cut(s) 93, 309
Bsh1236I CGCG 1 cut(s) 290
Bsh1285I CGRYCG 1 cut(s) 516
BshFI GGCC 3 cut(s) 65, 386, 515
BsiEI CGRYCG 1 cut(s) 516
BsiHKAI GWGCWC 2 cut(s) 300, 306
BsiHKCI CYCGRG 2 cut(s) 72, 299
BsiSI CCGG 3 cut(s) 66, 83, 207
BslFI GGGAC 2 cut(s) 26, 208
BslI CCNNNNNNNGG 2 cut(s) 77, 404
BsmAI GTCTC 1 cut(s) 69
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 26, 208
BsnI GGCC 3 cut(s) 65, 386, 515
BsoBI CYCGRG 2 cut(s) 72, 299
Bsp1286I GDGCHC 2 cut(s) 300, 306
Bsp143I GATC 2 cut(s) 42, 123
Bsp19I CCATGG 1 cut(s) 440
BspACI CCGC 7 cut(s) 47, 97, 177, 180, 282, 288, 377
BspANI GGCC 3 cut(s) 65, 386, 515
BspCNI CTCAG 2 cut(s) 249, 286
BspFNI CGCG 1 cut(s) 290
BspLI GGNNCC 5 cut(s) 14, 15, 70, 92, 167
BspPI GGATC 1 cut(s) 37
BsrBI CCGCTC 2 cut(s) 49, 379
BsrFI RCCGGY 2 cut(s) 82, 206
BsrI ACTGG 1 cut(s) 256
BssAI RCCGGY 2 cut(s) 82, 206
BssECI CCNNGG 2 cut(s) 60, 440
BssMI GATC 2 cut(s) 42, 123
BssT1I CCWWGG 2 cut(s) 60, 440
Bst4CI ACNGT 1 cut(s) 364
BstBAI YACGTR 1 cut(s) 213
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 3 cut(s) 236, 294, 522
BstDSI CCRYGG 1 cut(s) 440
BstFNI CGCG 1 cut(s) 290
BstHHI GCGC 1 cut(s) 292
BstKTI GATC 2 cut(s) 45, 126
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 2 cut(s) 42, 123
BstMCI CGRYCG 1 cut(s) 516
BstMWI GCNNNNNNNGC 3 cut(s) 310, 373, 512
BstNSI RCATGY 1 cut(s) 419
BstUI CGCG 1 cut(s) 290
BstV1I GCAGC 2 cut(s) 461, 515
BstX2I RGATCY 1 cut(s) 42
BstXI CCANNNNNNTGG 2 cut(s) 317, 469
BstYI RGATCY 1 cut(s) 42
BstZI CGGCCG 1 cut(s) 513
Bsu36I CCTNAGG 1 cut(s) 236
BsuRI GGCC 3 cut(s) 65, 386, 515
BtgI CCRYGG 1 cut(s) 440
Cac8I GCNNGC 1 cut(s) 447
CfoI GCGC 1 cut(s) 292
Cfr10I RCCGGY 2 cut(s) 82, 206
Cfr13I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 384
CpoI CGGWCCG 1 cut(s) 80
CseI GACGC 1 cut(s) 40
Csp6I GTAC 2 cut(s) 112, 220
CspI CGGWCCG 1 cut(s) 80
CviAII CATG 2 cut(s) 416, 441
CviJI RGCY 9 cut(s) 65, 100, 298, 304, 313, 376, 386, 445, 515
CviKI_1 RGCY 9 cut(s) 65, 100, 298, 304, 313, 376, 386, 445, 515
CviQI GTAC 2 cut(s) 112, 220
DdeI CTNAG 3 cut(s) 236, 294, 522
DpnI GATC 2 cut(s) 44, 125
DpnII GATC 2 cut(s) 42, 123
EaeI YGGCCR 1 cut(s) 513
EagI CGGCCG 1 cut(s) 513
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 2 cut(s) 298, 304
EclXI CGGCCG 1 cut(s) 513
Eco130I CCWWGG 2 cut(s) 60, 440
Eco24I GRGCYC 2 cut(s) 300, 306
Eco47I GGWCC 5 cut(s) 13, 68, 80, 90, 204
Eco52I CGGCCG 1 cut(s) 513
Eco53kI GAGCTC 2 cut(s) 298, 304
Eco72I CACGTG 1 cut(s) 213
Eco81I CCTNAGG 1 cut(s) 236
Eco88I CYCGRG 2 cut(s) 72, 299
EcoICRI GAGCTC 2 cut(s) 298, 304
EcoO109I RGGNCCY 1 cut(s) 13
EcoT14I CCWWGG 2 cut(s) 60, 440
EcoT38I GRGCYC 2 cut(s) 300, 306
ErhI CCWWGG 2 cut(s) 60, 440
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 2 cut(s) 419, 444
FaiI YATR 6 cut(s) 243, 339, 410, 417, 442, 482
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 2 cut(s) 26, 208
FatI CATG 2 cut(s) 415, 440
FauI CCCGC 2 cut(s) 54, 289
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 5 cut(s) 98, 180, 377, 450, 504
FriOI GRGCYC 2 cut(s) 300, 306
Fsp4HI GCNGC 5 cut(s) 98, 180, 377, 450, 504
FspBI CTAG 1 cut(s) 399
GlaI GCGC 1 cut(s) 291
GluI GCNGC 5 cut(s) 98, 180, 377, 450, 504
GsaI CCCAGC 2 cut(s) 97, 313
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 3 cut(s) 65, 386, 515
HapII CCGG 3 cut(s) 66, 83, 207
HgaI GACGC 1 cut(s) 40
HhaI GCGC 1 cut(s) 292
Hin1II CATG 2 cut(s) 419, 444
Hin6I GCGC 1 cut(s) 290
HinP1I GCGC 1 cut(s) 290
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 4 cut(s) 171, 457, 473, 518
HpaII CCGG 3 cut(s) 66, 83, 207
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 3 cut(s) 30, 90, 226
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 2 cut(s) 52, 188
Hpy8I GTNNAC 3 cut(s) 30, 90, 226
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 3 cut(s) 199, 212, 222
HpyCH4V TGCA 3 cut(s) 367, 449, 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 310, 373, 512
HpyF3I CTNAG 3 cut(s) 236, 294, 522
HpySE526I ACGT 3 cut(s) 199, 212, 222
Hsp92II CATG 2 cut(s) 419, 444
HspAI GCGC 1 cut(s) 290
KflI GGGWCCC 1 cut(s) 13
Kzo9I GATC 2 cut(s) 42, 123
LmnI GCTCC 3 cut(s) 54, 309, 442
Lsp1109I GCAGC 2 cut(s) 461, 515
LweI GCATC 1 cut(s) 490
MaeI CTAG 1 cut(s) 399
MaeII ACGT 3 cut(s) 199, 212, 222
MaeIII GTNAC 1 cut(s) 271
MalI GATC 2 cut(s) 44, 125
MbiI CCGCTC 2 cut(s) 49, 379
MboI GATC 2 cut(s) 42, 123
MboII GAAGA 1 cut(s) 361
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 2 cut(s) 300, 306
MmeI TCCRAC 2 cut(s) 174, 182
MnlI CCTC 6 cut(s) 100, 242, 245, 263, 335, 338
MslI CAYNNNNRTG 3 cut(s) 137, 467, 495
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 3 cut(s) 66, 83, 207
MvnI CGCG 1 cut(s) 290
MwoI GCNNNNNNNGC 3 cut(s) 310, 373, 512
NcoI CCATGG 1 cut(s) 440
NdeII GATC 2 cut(s) 42, 123
NlaIII CATG 2 cut(s) 419, 444
NlaIV GGNNCC 5 cut(s) 14, 15, 70, 92, 167
NmuCI GTSAC 1 cut(s) 271
NspI RCATGY 1 cut(s) 419
PaeR7I CTCGAG 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 267
PfeI GAWTC 4 cut(s) 171, 457, 473, 518
PkrI GCNGC 5 cut(s) 99, 181, 378, 451, 505
PmaCI CACGTG 1 cut(s) 213
PmlI CACGTG 1 cut(s) 213
Ppu21I YACGTR 1 cut(s) 213
PpuMI RGGWCCY 1 cut(s) 13
Psp124BI GAGCTC 2 cut(s) 300, 306
Psp5II RGGWCCY 1 cut(s) 13
PspCI CACGTG 1 cut(s) 213
PspFI CCCAGC 2 cut(s) 93, 309
PspN4I GGNNCC 5 cut(s) 14, 15, 70, 92, 167
PspPI GGNCC 6 cut(s) 13, 68, 80, 90, 204, 384
PspPPI RGGWCCY 1 cut(s) 13
PspXI VCTCGAGB 1 cut(s) 299
PsuI RGATCY 1 cut(s) 42
RsaI GTAC 2 cut(s) 113, 221
RsaNI GTAC 2 cut(s) 112, 220
RseI CAYNNNNRTG 3 cut(s) 137, 467, 495
Rsr2I CGGWCCG 1 cut(s) 80
RsrII CGGWCCG 1 cut(s) 80
SacI GAGCTC 2 cut(s) 300, 306
SalI GTCGAC 1 cut(s) 28
SatI GCNGC 5 cut(s) 98, 180, 377, 450, 504
Sau3AI GATC 2 cut(s) 42, 123
Sau96I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 384
SduI GDGCHC 2 cut(s) 300, 306
SfaNI GCATC 1 cut(s) 490
Sfr274I CTCGAG 1 cut(s) 299
SinI GGWCC 5 cut(s) 13, 68, 80, 90, 204
SlaI CTCGAG 1 cut(s) 299
SmiMI CAYNNNNRTG 3 cut(s) 137, 467, 495
SmlI CTYRAG 1 cut(s) 299
SmoI CTYRAG 1 cut(s) 299
SsiI CCGC 7 cut(s) 47, 97, 177, 180, 282, 288, 377
SspMI CTAG 1 cut(s) 399
SstI GAGCTC 2 cut(s) 300, 306
StyI CCWWGG 2 cut(s) 60, 440
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 3 cut(s) 202, 215, 225
TaqI TCGA 5 cut(s) 29, 249, 261, 300, 315
TatI WGTACW 1 cut(s) 111
TauI GCSGC 3 cut(s) 100, 182, 379
TfiI GAWTC 4 cut(s) 171, 457, 473, 518
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 2 cut(s) 449, 503
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 1 cut(s) 93
VpaK11BI GGWCC 5 cut(s) 13, 68, 80, 90, 204
XceI RCATGY 1 cut(s) 419
XhoI CTCGAG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.