Rh7CG450300

E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0011875
Physical Location & Seq
Forward (+)
59264163 .. 59266903
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG450300.1

Sequence Viewer

Length: 525 bp
ATGGAGGGTTGGGACCCGAACACGAAGTCGACGCTGACTCAGATCCCTCTCCTGACCACCAAGGCCGGGCCGCGAGATGGGCCGGCGTGGACCCAGAGGCTGAAAGAGGAGTACAAGTCGCTGATCGCCTACACCCAGATGAACAAGTCCAACGACAACGATTGGTTCCGAATCTCCGCCGCCAATCCCGAAGGGACACGTTGGACCGGCAAGTGCTGGTACGTCCACAACCTCCTCAAGTACGAGTTCGACCTCCAGTTCGATATTCCGGTCACTTACCCTGCCACCGCGCCGGAGCTCGAGCTCCCACAGCTCGATGGAAAAACCCAAAAGATGTATAGAGGAGGGAAGATTTGCTTGACCGTGCATTTCAAGCCGCTTTGGGCCAAAAACTGCCCTAGATTTGGTATAGCGCATGCTCTCTGTTTGGGTCTTGCTCCATGGCTTGCAGCAGAGATACCCATTCTTGTCGATTCTGGTATGGTCAAGCACAAAGATGATGCAGCAACATCGGCTGAATCTTAG

Protein Analysis

174

Amino Acids

19.62

Weight (kDa)

7.66

Isoelectric Point (pI)

30.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFC1 PF08694 8 - 163 3.3e-84 Ubiquitin-fold modifier-conjugating enzyme 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27530
fragaria_vesca FvH4_5g33720 FvH4_5g33720
malus_domestica MD05G1007500.v1.1 MD08G1209200.v1.1 MD15G1393200.v1.1
prunus_persica Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1
pyrus_communis pycom05g10270 pycom08g18060 pycom15g35350
rosa_chinensis RchiOBHm_Chr7g0232851
rosa_laevigata RLG00000001313
rosa_multiflora Rmu_ssc0000018.1_g000017
rosa_roxburghii Rroxscaffold_3G00227850
rosa_rugosa Rorug07G0276400
rosa_samantha Rh7CG450300
rosa_wichuraiana Rw7G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 29
AccII CGCG 2 cut(s) 73, 290
AciI CCGC 5 cut(s) 71, 177, 180, 288, 377
AclWI GGATC 1 cut(s) 37
AfaI GTAC 3 cut(s) 113, 221, 242
AfiI CCNNNNNNNGG 3 cut(s) 66, 77, 404
AflIII ACRYGT 1 cut(s) 197
AgsI TTSAA 1 cut(s) 373
AluBI AGCT 3 cut(s) 298, 304, 313
AluI AGCT 3 cut(s) 298, 304, 313
Alw21I GWGCWC 2 cut(s) 300, 306
AlwI GGATC 1 cut(s) 37
AlwNI CAGNNNCTG 1 cut(s) 100
Ama87I CYCGRG 1 cut(s) 299
AoxI GGCC 4 cut(s) 63, 68, 80, 384
ApeKI GCWGC 2 cut(s) 449, 503
ArsI GACNNNNNNTTYG 4 cut(s) 11, 43, 242, 274
AspLEI GCGC 2 cut(s) 292, 415
AspS9I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 384
AsuC2I CCSGG 1 cut(s) 67
AvaI CYCGRG 1 cut(s) 299
AvaII GGWCC 3 cut(s) 13, 90, 204
BanII GRGCYC 2 cut(s) 300, 306
Bbv12I GWGCWC 2 cut(s) 300, 306
BbvI GCAGC 2 cut(s) 461, 515
BccI CCATC 2 cut(s) 71, 311
BcgI CGANNNNNNTGC 1 cut(s) 492
BcnI CCSGG 1 cut(s) 67
BfaI CTAG 1 cut(s) 399
BisI GCNGC 5 cut(s) 71, 180, 377, 450, 504
BlsI GCNGC 5 cut(s) 72, 181, 378, 451, 505
Bme1390I CCNGG 1 cut(s) 67
Bme18I GGWCC 3 cut(s) 13, 90, 204
BmeT110I CYCGRG 1 cut(s) 299
BmgT120I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 384
BmiI GGNNCC 4 cut(s) 14, 15, 92, 167
BmrFI CCNGG 1 cut(s) 67
BmsI GCATC 1 cut(s) 490
BpmI CTGGAG 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 67
BsaJI CCNNGG 2 cut(s) 60, 440
BsaWI WCCGGW 1 cut(s) 268
BsaXI ACNNNNNCTCC 2 cut(s) 101, 131
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 77, 404
Bse118I RCCGGY 2 cut(s) 82, 206
Bse1I ACTGG 1 cut(s) 256
BseDI CCNNGG 2 cut(s) 60, 440
BseLI CCNNNNNNNGG 3 cut(s) 66, 77, 404
BseMII CTCAG 1 cut(s) 53
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 3 cut(s) 122, 224, 357
BseXI GCAGC 2 cut(s) 461, 515
Bsh1236I CGCG 2 cut(s) 73, 290
BshFI GGCC 4 cut(s) 65, 70, 82, 386
BsiHKAI GWGCWC 2 cut(s) 300, 306
BsiHKCI CYCGRG 1 cut(s) 299
BsiSI CCGG 5 cut(s) 66, 83, 207, 269, 293
BslFI GGGAC 2 cut(s) 26, 208
BslI CCNNNNNNNGG 3 cut(s) 66, 77, 404
BsmFI GGGAC 2 cut(s) 26, 208
BsnI GGCC 4 cut(s) 65, 70, 82, 386
BsoBI CYCGRG 1 cut(s) 299
Bsp1286I GDGCHC 2 cut(s) 300, 306
Bsp143I GATC 2 cut(s) 42, 123
Bsp19I CCATGG 1 cut(s) 440
BspACI CCGC 5 cut(s) 71, 177, 180, 288, 377
BspANI GGCC 4 cut(s) 65, 70, 82, 386
BspCNI CTCAG 1 cut(s) 52
BspFNI CGCG 2 cut(s) 73, 290
BspLI GGNNCC 4 cut(s) 14, 15, 92, 167
BspPI GGATC 1 cut(s) 37
BsrFI RCCGGY 2 cut(s) 82, 206
BsrI ACTGG 1 cut(s) 256
BssAI RCCGGY 2 cut(s) 82, 206
BssECI CCNNGG 2 cut(s) 60, 440
BssMI GATC 2 cut(s) 42, 123
BssT1I CCWWGG 2 cut(s) 60, 440
Bst4CI ACNGT 1 cut(s) 364
BstC8I GCNNGC 3 cut(s) 84, 417, 447
BstDEI CTNAG 2 cut(s) 39, 522
BstDSI CCRYGG 1 cut(s) 440
BstFNI CGCG 2 cut(s) 73, 290
BstHHI GCGC 2 cut(s) 292, 415
BstKTI GATC 2 cut(s) 45, 126
BstMBI GATC 2 cut(s) 42, 123
BstMWI GCNNNNNNNGC 4 cut(s) 79, 310, 373, 512
BstNSI RCATGY 1 cut(s) 419
BstSCI CCNGG 1 cut(s) 65
BstUI CGCG 2 cut(s) 73, 290
BstV1I GCAGC 2 cut(s) 461, 515
BstX2I RGATCY 1 cut(s) 42
BstYI RGATCY 1 cut(s) 42
BsuRI GGCC 4 cut(s) 65, 70, 82, 386
BtgI CCRYGG 1 cut(s) 440
Cac8I GCNNGC 3 cut(s) 84, 417, 447
CaiI CAGNNNCTG 1 cut(s) 100
CfoI GCGC 2 cut(s) 292, 415
Cfr10I RCCGGY 2 cut(s) 82, 206
Cfr13I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 384
CseI GACGC 1 cut(s) 40
Csp6I GTAC 3 cut(s) 112, 220, 241
CviAII CATG 2 cut(s) 416, 441
CviQI GTAC 3 cut(s) 112, 220, 241
DdeI CTNAG 2 cut(s) 39, 522
DpnI GATC 2 cut(s) 44, 125
DpnII GATC 2 cut(s) 42, 123
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 2 cut(s) 298, 304
Eco130I CCWWGG 2 cut(s) 60, 440
Eco24I GRGCYC 2 cut(s) 300, 306
Eco47I GGWCC 3 cut(s) 13, 90, 204
Eco53kI GAGCTC 2 cut(s) 298, 304
Eco88I CYCGRG 1 cut(s) 299
EcoICRI GAGCTC 2 cut(s) 298, 304
EcoO109I RGGNCCY 1 cut(s) 13
EcoT14I CCWWGG 2 cut(s) 60, 440
EcoT38I GRGCYC 2 cut(s) 300, 306
ErhI CCWWGG 2 cut(s) 60, 440
FaeI CATG 2 cut(s) 419, 444
FaiI YATR 5 cut(s) 339, 410, 417, 442, 482
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 2 cut(s) 26, 208
FatI CATG 2 cut(s) 415, 440
FblI GTMKAC 1 cut(s) 29
Fnu4HI GCNGC 5 cut(s) 71, 180, 377, 450, 504
FriOI GRGCYC 2 cut(s) 300, 306
Fsp4HI GCNGC 5 cut(s) 71, 180, 377, 450, 504
FspBI CTAG 1 cut(s) 399
GlaI GCGC 2 cut(s) 291, 414
GluI GCNGC 5 cut(s) 71, 180, 377, 450, 504
GsuI CTGGAG 1 cut(s) 239
HaeIII GGCC 4 cut(s) 65, 70, 82, 386
HapII CCGG 5 cut(s) 66, 83, 207, 269, 293
HgaI GACGC 1 cut(s) 40
HhaI GCGC 2 cut(s) 292, 415
Hin1II CATG 2 cut(s) 419, 444
Hin6I GCGC 2 cut(s) 290, 413
HinP1I GCGC 2 cut(s) 290, 413
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 4 cut(s) 37, 171, 473, 518
HpaII CCGG 5 cut(s) 66, 83, 207, 269, 293
Hpy166II GTNNAC 3 cut(s) 30, 90, 226
Hpy188I TCNGA 2 cut(s) 42, 170
Hpy188III TCNNGA 2 cut(s) 52, 188
Hpy8I GTNNAC 3 cut(s) 30, 90, 226
Hpy99I CGWCG 1 cut(s) 34
HpyAV CCTTC 1 cut(s) 185
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 2 cut(s) 199, 222
HpyCH4V TGCA 3 cut(s) 367, 449, 503
HpyF10VI GCNNNNNNNGC 4 cut(s) 79, 310, 373, 512
HpyF3I CTNAG 2 cut(s) 39, 522
HpySE526I ACGT 2 cut(s) 199, 222
Hsp92II CATG 2 cut(s) 419, 444
HspAI GCGC 2 cut(s) 290, 413
KflI GGGWCCC 1 cut(s) 13
KroI GCCGGC 1 cut(s) 82
KroNI GCCGGC 1 cut(s) 84
Kzo9I GATC 2 cut(s) 42, 123
LmnI GCTCC 3 cut(s) 295, 309, 442
Lsp1109I GCAGC 2 cut(s) 461, 515
LweI GCATC 1 cut(s) 490
MaeI CTAG 1 cut(s) 399
MaeII ACGT 2 cut(s) 199, 222
MaeIII GTNAC 1 cut(s) 271
MalI GATC 2 cut(s) 44, 125
MboI GATC 2 cut(s) 42, 123
MboII GAAGA 1 cut(s) 361
MflI RGATCY 1 cut(s) 42
MhlI GDGCHC 2 cut(s) 300, 306
MlyI GAGTC 1 cut(s) 31
MmeI TCCRAC 2 cut(s) 174, 182
MnlI CCTC 8 cut(s) 57, 90, 100, 242, 245, 263, 335, 338
MroNI GCCGGC 1 cut(s) 82
MslI CAYNNNNRTG 2 cut(s) 137, 495
MspI CCGG 5 cut(s) 66, 83, 207, 269, 293
MspR9I CCNGG 1 cut(s) 67
MvnI CGCG 2 cut(s) 73, 290
MwoI GCNNNNNNNGC 4 cut(s) 79, 310, 373, 512
NaeI GCCGGC 1 cut(s) 84
NciI CCSGG 1 cut(s) 67
NcoI CCATGG 1 cut(s) 440
NdeII GATC 2 cut(s) 42, 123
NgoMIV GCCGGC 1 cut(s) 82
NlaIII CATG 2 cut(s) 419, 444
NlaIV GGNNCC 4 cut(s) 14, 15, 92, 167
NmuCI GTSAC 1 cut(s) 271
NspI RCATGY 1 cut(s) 419
PaeI GCATGC 1 cut(s) 419
PaeR7I CTCGAG 1 cut(s) 299
PdiI GCCGGC 1 cut(s) 84
PfeI GAWTC 3 cut(s) 171, 473, 518
PkrI GCNGC 5 cut(s) 72, 181, 378, 451, 505
PleI GAGTC 1 cut(s) 31
PpsI GAGTC 1 cut(s) 31
PpuMI RGGWCCY 1 cut(s) 13
Psp124BI GAGCTC 2 cut(s) 300, 306
Psp5II RGGWCCY 1 cut(s) 13
PspN4I GGNNCC 4 cut(s) 14, 15, 92, 167
PspPI GGNCC 6 cut(s) 13, 68, 80, 90, 204, 384
PspPPI RGGWCCY 1 cut(s) 13
PspXI VCTCGAGB 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 100
PsuI RGATCY 1 cut(s) 42
RsaI GTAC 3 cut(s) 113, 221, 242
RsaNI GTAC 3 cut(s) 112, 220, 241
RseI CAYNNNNRTG 2 cut(s) 137, 495
SacI GAGCTC 2 cut(s) 300, 306
SalI GTCGAC 1 cut(s) 28
SatI GCNGC 5 cut(s) 71, 180, 377, 450, 504
Sau3AI GATC 2 cut(s) 42, 123
Sau96I GGNCC 6 cut(s) 13, 68, 80, 90, 204, 384
SchI GAGTC 1 cut(s) 31
ScrFI CCNGG 1 cut(s) 67
SduI GDGCHC 2 cut(s) 300, 306
SetI ASST 7 cut(s) 202, 225, 234, 255, 300, 306, 315
SfaNI GCATC 1 cut(s) 490
Sfr274I CTCGAG 1 cut(s) 299
SinI GGWCC 3 cut(s) 13, 90, 204
SlaI CTCGAG 1 cut(s) 299
SmiMI CAYNNNNRTG 2 cut(s) 137, 495
SmlI CTYRAG 2 cut(s) 236, 299
SmoI CTYRAG 2 cut(s) 236, 299
SphI GCATGC 1 cut(s) 419
SsiI CCGC 5 cut(s) 71, 177, 180, 288, 377
SspMI CTAG 1 cut(s) 399
SstI GAGCTC 2 cut(s) 300, 306
StyD4I CCNGG 1 cut(s) 65
StyI CCWWGG 2 cut(s) 60, 440
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 2 cut(s) 202, 225
TaqI TCGA 6 cut(s) 29, 249, 261, 300, 315, 471
TatI WGTACW 1 cut(s) 111
TauI GCSGC 3 cut(s) 73, 182, 379
TfiI GAWTC 3 cut(s) 171, 473, 518
TseFI GTSAC 1 cut(s) 271
TseI GCWGC 2 cut(s) 449, 503
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 1 cut(s) 155
VpaK11BI GGWCC 3 cut(s) 13, 90, 204
XceI RCATGY 1 cut(s) 419
XhoI CTCGAG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 29
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.