MD15G1393200.v1.1

E2-like enzyme which forms an intermediate with UFM1 via a thioester linkage

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011875
Physical Location & Seq
Reverse (-)
49018723 .. 49020581
1859 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1393200.v1.1.491

Sequence Viewer

Length: 525 bp
ATGGAGGGTTGGGACCCGAACACCAAGTCGACGCTGACCCAGATCCCACTCTTGACGACGAAGGCCGGTCCCAGAGATGGGGCGGCGTGGACGCAGCGGCTGAAGGAGGAGTACAAGGCGCTGATCGCATACACCCAGATGAACAAGTCCAACGACAACGATTGGTTCCGAATCTCCGCCGCCAATCCCGAGGGTACGCGGTGGACTGGCAAGTGCTGGTACGTCTACAACCTCCTCAAGTACGAGTTCGATCTCCAGTTCGATATCCCCATCACCTACCCCGCCACCGCACCGGAACTCGAGCTCCCTCAACTAGACGGCAAGACCCAAAAGATGTATAGAGGGGGGAAGATCTGCTTGACGGTGCATTTCAAACCGCTTTGGGCCAAGAACTGTCCAAGATTTGGTATAGCACATGCACTTTGTTTGGGTCTTGCACCATGGCTTGCAGCAGAGATTCCTGTTCTTGTGGATTCTGGTATGGTTAAGCACAAAGATGATGCTGCATCATCAACTGAATCTTAG

Protein Analysis

175

Amino Acids

19.62

Weight (kDa)

7.64

Isoelectric Point (pI)

29.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UFC1 PF08694 8 - 163 4.1e-84 Ubiquitin-fold modifier-conjugating enzyme 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011875)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G27530
fragaria_vesca FvH4_5g33720 FvH4_5g33720
malus_domestica MD05G1007500.v1.1 MD08G1209200.v1.1 MD15G1393200.v1.1
prunus_persica Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1 Prupe.1G541400_v2.0.a1
pyrus_communis pycom05g10270 pycom08g18060 pycom15g35350
rosa_chinensis RchiOBHm_Chr7g0232851
rosa_laevigata RLG00000001313
rosa_multiflora Rmu_ssc0000018.1_g000017
rosa_roxburghii Rroxscaffold_3G00227850
rosa_rugosa Rorug07G0276400
rosa_samantha Rh7CG450300
rosa_wichuraiana Rw7G035630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 404
AccI GTMKAC 2 cut(s) 29, 225
AccII CGCG 1 cut(s) 199
AciI CCGC 8 cut(s) 83, 97, 177, 180, 199, 282, 288, 377
AclWI GGATC 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 122
AfaI GTAC 4 cut(s) 113, 196, 221, 242
AfiI CCNNNNNNNGG 3 cut(s) 77, 78, 404
AgsI TTSAA 1 cut(s) 373
AloI GAACNNNNNNTCC 2 cut(s) 288, 320
AluBI AGCT 1 cut(s) 304
AluI AGCT 1 cut(s) 304
Alw21I GWGCWC 1 cut(s) 306
AlwI GGATC 1 cut(s) 37
AlwNI CAGNNNCTG 1 cut(s) 100
Ama87I CYCGRG 2 cut(s) 188, 299
AoxI GGCC 2 cut(s) 63, 384
ApeKI GCWGC 3 cut(s) 94, 449, 503
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 3 cut(s) 13, 68, 384
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 2 cut(s) 188, 299
AvaII GGWCC 2 cut(s) 13, 68
BanII GRGCYC 1 cut(s) 306
BarI GAAGNNNNNNTAC 2 cut(s) 95, 127
Bbv12I GWGCWC 1 cut(s) 306
BbvI GCAGC 3 cut(s) 106, 461, 490
BccI CCATC 2 cut(s) 71, 278
BceAI ACGGC 1 cut(s) 334
BfaI CTAG 1 cut(s) 314
BfoI RGCGCY 1 cut(s) 122
BglII AGATCT 1 cut(s) 351
BisI GCNGC 6 cut(s) 84, 95, 98, 180, 450, 504
BlsI GCNGC 6 cut(s) 85, 96, 99, 181, 451, 505
Bme18I GGWCC 2 cut(s) 13, 68
BmeT110I CYCGRG 2 cut(s) 188, 299
BmgT120I GGNCC 3 cut(s) 13, 68, 384
BmiI GGNNCC 4 cut(s) 14, 15, 70, 167
BmsI GCATC 2 cut(s) 490, 515
BpmI CTGGAG 1 cut(s) 239
BpuEI CTTGAG 1 cut(s) 221
BsaJI CCNNGG 2 cut(s) 189, 440
BsaWI WCCGGW 1 cut(s) 292
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 78, 404
Bse118I RCCGGY 1 cut(s) 65
Bse1I ACTGG 2 cut(s) 211, 256
BseDI CCNNGG 2 cut(s) 189, 440
BseLI CCNNNNNNNGG 3 cut(s) 77, 78, 404
BseNI ACTGG 2 cut(s) 211, 256
BseRI GAGGAG 2 cut(s) 122, 224
BseXI GCAGC 3 cut(s) 106, 461, 490
Bsh1236I CGCG 1 cut(s) 199
BshFI GGCC 2 cut(s) 65, 386
BsiHKAI GWGCWC 1 cut(s) 306
BsiHKCI CYCGRG 2 cut(s) 188, 299
BsiSI CCGG 2 cut(s) 66, 293
BslFI GGGAC 2 cut(s) 26, 54
BslI CCNNNNNNNGG 3 cut(s) 77, 78, 404
BsmFI GGGAC 2 cut(s) 26, 54
BsnI GGCC 2 cut(s) 65, 386
BsoBI CYCGRG 2 cut(s) 188, 299
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 4 cut(s) 42, 123, 250, 351
Bsp19I CCATGG 1 cut(s) 440
BspACI CCGC 8 cut(s) 83, 97, 177, 180, 199, 282, 288, 377
BspANI GGCC 2 cut(s) 65, 386
BspFNI CGCG 1 cut(s) 199
BspLI GGNNCC 4 cut(s) 14, 15, 70, 167
BspPI GGATC 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 65
BsrI ACTGG 2 cut(s) 211, 256
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 189, 440
BssMI GATC 4 cut(s) 42, 123, 250, 351
BssT1I CCWWGG 1 cut(s) 440
Bst4CI ACNGT 2 cut(s) 364, 395
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 1 cut(s) 522
BstDSI CCRYGG 1 cut(s) 440
BstFNI CGCG 1 cut(s) 199
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 4 cut(s) 45, 126, 253, 354
BstMBI GATC 4 cut(s) 42, 123, 250, 351
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstNSI RCATGY 1 cut(s) 419
BstUI CGCG 1 cut(s) 199
BstV1I GCAGC 3 cut(s) 106, 461, 490
BstX2I RGATCY 2 cut(s) 42, 351
BstYI RGATCY 2 cut(s) 42, 351
BsuRI GGCC 2 cut(s) 65, 386
BtgI CCRYGG 1 cut(s) 440
Cac8I GCNNGC 1 cut(s) 447
CaiI CAGNNNCTG 1 cut(s) 100
CfoI GCGC 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 3 cut(s) 13, 68, 384
CseI GACGC 2 cut(s) 40, 100
Csp6I GTAC 4 cut(s) 112, 195, 220, 241
CviAII CATG 2 cut(s) 416, 441
CviJI RGCY 5 cut(s) 65, 100, 304, 386, 445
CviKI_1 RGCY 5 cut(s) 65, 100, 304, 386, 445
CviQI GTAC 4 cut(s) 112, 195, 220, 241
DdeI CTNAG 1 cut(s) 522
DpnI GATC 4 cut(s) 44, 125, 252, 353
DpnII GATC 4 cut(s) 42, 123, 250, 351
EciI GGCGGA 1 cut(s) 166
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 306
Eco32I GATATC 1 cut(s) 265
Eco47I GGWCC 2 cut(s) 13, 68
Eco53kI GAGCTC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 122
Eco88I CYCGRG 2 cut(s) 188, 299
EcoICRI GAGCTC 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 13
EcoRV GATATC 1 cut(s) 265
EcoT14I CCWWGG 1 cut(s) 440
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 440
FaeI CATG 2 cut(s) 419, 444
FaiI YATR 6 cut(s) 130, 339, 410, 417, 442, 482
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 2 cut(s) 26, 54
FatI CATG 2 cut(s) 415, 440
FauI CCCGC 1 cut(s) 289
FblI GTMKAC 2 cut(s) 29, 225
Fnu4HI GCNGC 6 cut(s) 84, 95, 98, 180, 450, 504
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 6 cut(s) 84, 95, 98, 180, 450, 504
FspBI CTAG 1 cut(s) 314
GlaI GCGC 1 cut(s) 120
GluI GCNGC 6 cut(s) 84, 95, 98, 180, 450, 504
GsuI CTGGAG 1 cut(s) 239
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 2 cut(s) 65, 386
HapII CCGG 2 cut(s) 66, 293
HgaI GACGC 2 cut(s) 40, 100
HhaI GCGC 1 cut(s) 121
Hin1II CATG 2 cut(s) 419, 444
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HincII GTYRAC 1 cut(s) 30
HindII GTYRAC 1 cut(s) 30
HinfI GANTC 4 cut(s) 171, 457, 473, 518
HpaII CCGG 2 cut(s) 66, 293
HphI GGTGA 1 cut(s) 265
Hpy166II GTNNAC 4 cut(s) 30, 90, 204, 226
Hpy188I TCNGA 1 cut(s) 170
Hpy188III TCNNGA 2 cut(s) 52, 188
Hpy8I GTNNAC 4 cut(s) 30, 90, 204, 226
Hpy99I CGWCG 2 cut(s) 34, 61
HpyAV CCTTC 2 cut(s) 55, 97
HpyCH4III ACNGT 2 cut(s) 364, 395
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 5 cut(s) 367, 419, 437, 449, 506
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 1 cut(s) 522
HpySE526I ACGT 1 cut(s) 222
Hsp92II CATG 2 cut(s) 419, 444
HspAI GCGC 1 cut(s) 119
KflI GGGWCCC 1 cut(s) 13
Kzo9I GATC 4 cut(s) 42, 123, 250, 351
LmnI GCTCC 1 cut(s) 309
Lsp1109I GCAGC 3 cut(s) 106, 461, 490
LweI GCATC 2 cut(s) 490, 515
MaeI CTAG 1 cut(s) 314
MaeII ACGT 1 cut(s) 222
MalI GATC 4 cut(s) 44, 125, 252, 353
MboI GATC 4 cut(s) 42, 123, 250, 351
MboII GAAGA 1 cut(s) 361
MflI RGATCY 2 cut(s) 42, 351
MhlI GDGCHC 1 cut(s) 306
MmeI TCCRAC 1 cut(s) 174
MnlI CCTC 6 cut(s) 100, 184, 242, 245, 318, 335
MseI TTAA 1 cut(s) 486
MslI CAYNNNNRTG 2 cut(s) 137, 495
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 2 cut(s) 66, 293
MvnI CGCG 1 cut(s) 199
MwoI GCNNNNNNNGC 1 cut(s) 125
NcoI CCATGG 1 cut(s) 440
NdeII GATC 4 cut(s) 42, 123, 250, 351
NlaIII CATG 2 cut(s) 419, 444
NlaIV GGNNCC 4 cut(s) 14, 15, 70, 167
NspI RCATGY 1 cut(s) 419
PaeR7I CTCGAG 1 cut(s) 299
PfeI GAWTC 4 cut(s) 171, 457, 473, 518
PflMI CCANNNNNTGG 1 cut(s) 404
PkrI GCNGC 6 cut(s) 85, 96, 99, 181, 451, 505
PpuMI RGGWCCY 1 cut(s) 13
Psp124BI GAGCTC 1 cut(s) 306
Psp5II RGGWCCY 1 cut(s) 13
PspN4I GGNNCC 4 cut(s) 14, 15, 70, 167
PspPI GGNCC 3 cut(s) 13, 68, 384
PspPPI RGGWCCY 1 cut(s) 13
PspXI VCTCGAGB 1 cut(s) 299
PstNI CAGNNNCTG 1 cut(s) 100
PsuI RGATCY 2 cut(s) 42, 351
RsaI GTAC 4 cut(s) 113, 196, 221, 242
RsaNI GTAC 4 cut(s) 112, 195, 220, 241
RseI CAYNNNNRTG 2 cut(s) 137, 495
SacI GAGCTC 1 cut(s) 306
SalI GTCGAC 1 cut(s) 28
SaqAI TTAA 1 cut(s) 486
SatI GCNGC 6 cut(s) 84, 95, 98, 180, 450, 504
Sau3AI GATC 4 cut(s) 42, 123, 250, 351
Sau96I GGNCC 3 cut(s) 13, 68, 384
SduI GDGCHC 1 cut(s) 306
SetI ASST 4 cut(s) 225, 234, 278, 306
SfaNI GCATC 2 cut(s) 490, 515
Sfr274I CTCGAG 1 cut(s) 299
SinI GGWCC 2 cut(s) 13, 68
SlaI CTCGAG 1 cut(s) 299
SmiMI CAYNNNNRTG 2 cut(s) 137, 495
SmlI CTYRAG 2 cut(s) 236, 299
SmoI CTYRAG 2 cut(s) 236, 299
SsiI CCGC 8 cut(s) 83, 97, 177, 180, 199, 282, 288, 377
SspMI CTAG 1 cut(s) 314
SstI GAGCTC 1 cut(s) 306
StyI CCWWGG 1 cut(s) 440
TaaI ACNGT 2 cut(s) 364, 395
TaiI ACGT 1 cut(s) 225
TaqI TCGA 4 cut(s) 29, 249, 261, 300
TatI WGTACW 1 cut(s) 111
TauI GCSGC 3 cut(s) 86, 100, 182
TfiI GAWTC 4 cut(s) 171, 457, 473, 518
Tru1I TTAA 1 cut(s) 486
Tru9I TTAA 1 cut(s) 486
TseI GCWGC 3 cut(s) 94, 449, 503
TspDTI ATGAA 1 cut(s) 155
Van91I CCANNNNNTGG 1 cut(s) 404
VpaK11BI GGWCC 2 cut(s) 13, 68
XceI RCATGY 1 cut(s) 419
XhoI CTCGAG 1 cut(s) 299
XmiI GTMKAC 2 cut(s) 29, 225
XspI CTAG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.