AT1G29770

NLI interacting factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006661
Physical Location & Seq
Forward (+)
10424463 .. 10425801
1339 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G29770.1

Sequence Viewer

Length: 837 bp
ATGGCGTGTCATGGATTTCTGGGGAAACCGACGCCGAAATCATTTGGGCGTCATCATAAACATGCAAGGAAAAACCGGAATCATCAATCCAGATGTTTGGCTGCAGCCGTTGCAGCAGCTGGCTCCATCTTCACATCCCTCAACATGTCTATCTTCACATTCCACAACCGCCTCTTGCGTTGCGTCTCCAGGTTTTTGCGTCTCGCCACCACATCTAGCGCAACTCCTACCCGTCGACCCACCATGAAACAAGGCTACAAAAAACTCCATAAACGCGAGCCTCTGATAAGACGTAATGACAAGAAAAGGACAATCTTTCTTGATCTTGACGAAACATTGGTACATTCAACGATGGAACCACCAATCCGGGTTAATGTAGATTTCATGGTGAGGATAAAGATCGAAGGAGCCGTTATACCAATGTTTGTGGTAAAACGACCAGGAGTTACTGAGTTTCTTGAAAGGATCAGTAAGAACTATCGAGTGGCAATCTTCACAGCCGGGTTACCGGAATATGCTTCACAGGTTTTGGATAAACTTGACAAGAATCGTGTGATCTCACAGCGGCTATATCGAGATTCATGCACAGAAGTGAATGGAAGATATGCCAAAGACTTATCTTTGGTGGCGAAGAATGACCTCGGGAGTGTTTTGCTAGTTGACGATAACCCTTTCTCCTACTCGTTGCAGCCAGACAATGGAGTTCCCATTAAGCCATTTATGGATGACATGGAAGATCAAGAACTAATGAAGCTTGCAGAATTCTTCGATGGATGTTATCAGTACGAAGATCTAAGGGATGCAGCAGCAGAACTTCTATCCAGCAAGCTGATTTGA

Protein Analysis

278

Amino Acids

31.73

Weight (kDa)

9.6

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 104 - 266 2.2e-52 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 698
AccI GTMKAC 1 cut(s) 235
AccII CGCG 1 cut(s) 276
AciI CCGC 2 cut(s) 169, 565
AclWI GGATC 1 cut(s) 473
AcsI RAATTY 1 cut(s) 761
AcyI GRCGYC 2 cut(s) 32, 49
AfaI GTAC 2 cut(s) 342, 785
AfiI CCNNNNNNNGG 1 cut(s) 698
AflIII ACRYGT 1 cut(s) 144
AgsI TTSAA 2 cut(s) 348, 461
AjnI CCWGG 2 cut(s) 188, 439
AleI CACNNNNGTG 1 cut(s) 590
AloI GAACNNNNNNTCC 2 cut(s) 348, 380
AluBI AGCT 3 cut(s) 119, 754, 829
AluI AGCT 3 cut(s) 119, 754, 829
Alw26I GTCTC 2 cut(s) 190, 206
AlwI GGATC 1 cut(s) 473
AlwNI CAGNNNCTG 1 cut(s) 119
Ama87I CYCGRG 1 cut(s) 641
ApeKI GCWGC 7 cut(s) 101, 104, 113, 116, 688, 803, 806
ApoI RAATTY 1 cut(s) 761
AspLEI GCGC 1 cut(s) 221
AsuC2I CCSGG 2 cut(s) 368, 502
AsuHPI GGTGA 1 cut(s) 400
AvaI CYCGRG 1 cut(s) 641
BbvI GCAGC 7 cut(s) 88, 116, 125, 128, 700, 815, 818
BccI CCATC 3 cut(s) 134, 346, 764
BceAI ACGGC 2 cut(s) 92, 395
BcgI CGANNNNNNTGC 2 cut(s) 564, 598
BciT130I CCWGG 2 cut(s) 190, 441
BcnI CCSGG 2 cut(s) 368, 502
BcoDI GTCTC 2 cut(s) 190, 206
BfaI CTAG 2 cut(s) 216, 656
BfmI CTRYAG 1 cut(s) 102
BglII AGATCT 1 cut(s) 790
BisI GCNGC 8 cut(s) 102, 105, 114, 117, 566, 689, 804, 807
BlsI GCNGC 8 cut(s) 103, 106, 115, 118, 567, 690, 805, 808
Bme1390I CCNGG 4 cut(s) 190, 368, 441, 502
BmeT110I CYCGRG 1 cut(s) 641
BmiI GGNNCC 3 cut(s) 124, 357, 409
BmrFI CCNGG 4 cut(s) 190, 368, 441, 502
BmsI GCATC 1 cut(s) 790
BpmI CTGGAG 1 cut(s) 172
BpuMI CCSGG 2 cut(s) 368, 502
BsaBI GATNNNNATC 1 cut(s) 398
BsaHI GRCGYC 2 cut(s) 32, 49
BsaJI CCNNGG 1 cut(s) 640
BsaWI WCCGGW 2 cut(s) 75, 508
BsaXI ACNNNNNCTCC 2 cut(s) 659, 689
Bsc4I CCNNNNNNNGG 1 cut(s) 698
Bse8I GATNNNNATC 1 cut(s) 398
BseBI CCWGG 2 cut(s) 190, 441
BseDI CCNNGG 1 cut(s) 640
BseGI GGATG 4 cut(s) 134, 730, 779, 805
BseJI GATNNNNATC 1 cut(s) 398
BseLI CCNNNNNNNGG 1 cut(s) 698
BseMII CTCAG 1 cut(s) 441
BseXI GCAGC 7 cut(s) 88, 116, 125, 128, 700, 815, 818
Bsh1236I CGCG 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 4 cut(s) 76, 367, 501, 509
BslI CCNNNNNNNGG 1 cut(s) 698
BsmAI GTCTC 2 cut(s) 190, 206
BsmBI CGTCTC 2 cut(s) 190, 206
BsoBI CYCGRG 1 cut(s) 641
Bsp143I GATC 6 cut(s) 322, 399, 465, 555, 736, 790
BspACI CCGC 2 cut(s) 169, 565
BspCNI CTCAG 1 cut(s) 442
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 3 cut(s) 124, 357, 409
BspMAI CTGCAG 1 cut(s) 106
BspPI GGATC 1 cut(s) 473
BssECI CCNNGG 1 cut(s) 640
BssMI GATC 6 cut(s) 322, 399, 465, 555, 736, 790
BssNI GRCGYC 2 cut(s) 32, 49
Bst2UI CCWGG 2 cut(s) 190, 441
BstACI GRCGYC 2 cut(s) 32, 49
BstAPI GCANNNNNTGC 1 cut(s) 110
BstC8I GCNNGC 4 cut(s) 121, 278, 756, 827
BstDEI CTNAG 2 cut(s) 450, 794
BstEII GGTNACC 1 cut(s) 504
BstF5I GGATG 4 cut(s) 134, 730, 779, 805
BstFNI CGCG 1 cut(s) 276
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 6 cut(s) 325, 402, 468, 558, 739, 793
BstMAI GTCTC 2 cut(s) 190, 206
BstMBI GATC 6 cut(s) 322, 399, 465, 555, 736, 790
BstMWI GCNNNNNNNGC 2 cut(s) 110, 113
BstNI CCWGG 2 cut(s) 190, 441
BstNSI RCATGY 2 cut(s) 65, 148
BstPI GGTNACC 1 cut(s) 504
BstSCI CCNGG 4 cut(s) 188, 366, 439, 500
BstSFI CTRYAG 1 cut(s) 102
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 7 cut(s) 88, 116, 125, 128, 700, 815, 818
BstX2I RGATCY 1 cut(s) 790
BstXI CCANNNNNNTGG 1 cut(s) 97
BstYI RGATCY 1 cut(s) 790
BtsCI GGATG 4 cut(s) 134, 730, 779, 805
Cac8I GCNNGC 4 cut(s) 121, 278, 756, 827
CaiI CAGNNNCTG 1 cut(s) 119
CfoI GCGC 1 cut(s) 221
CseI GACGC 4 cut(s) 38, 40, 172, 188
Csp6I GTAC 2 cut(s) 341, 784
CspCI CAANNNNNGTGG 2 cut(s) 408, 443
CviAII CATG 7 cut(s) 11, 62, 145, 244, 385, 582, 730
CviQI GTAC 2 cut(s) 341, 784
DdeI CTNAG 2 cut(s) 450, 794
DpnI GATC 6 cut(s) 324, 401, 467, 557, 738, 792
DpnII GATC 6 cut(s) 322, 399, 465, 555, 736, 790
Eco88I CYCGRG 1 cut(s) 641
Eco91I GGTNACC 1 cut(s) 504
EcoO65I GGTNACC 1 cut(s) 504
EcoRI GAATTC 1 cut(s) 761
EcoRII CCWGG 2 cut(s) 188, 439
Esp3I CGTCTC 2 cut(s) 190, 206
FaeI CATG 7 cut(s) 14, 65, 148, 247, 388, 585, 733
FatI CATG 7 cut(s) 10, 61, 144, 243, 384, 581, 729
FblI GTMKAC 1 cut(s) 235
Fnu4HI GCNGC 8 cut(s) 102, 105, 114, 117, 566, 689, 804, 807
FokI GGATG 4 cut(s) 121, 737, 786, 812
Fsp4HI GCNGC 8 cut(s) 102, 105, 114, 117, 566, 689, 804, 807
FspBI CTAG 2 cut(s) 216, 656
GlaI GCGC 1 cut(s) 220
GluI GCNGC 8 cut(s) 102, 105, 114, 117, 566, 689, 804, 807
GsuI CTGGAG 1 cut(s) 172
HapII CCGG 4 cut(s) 76, 367, 501, 509
HgaI GACGC 4 cut(s) 38, 40, 172, 188
HhaI GCGC 1 cut(s) 221
Hin1I GRCGYC 2 cut(s) 32, 49
Hin1II CATG 7 cut(s) 14, 65, 148, 247, 388, 585, 733
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HincII GTYRAC 2 cut(s) 236, 661
HindII GTYRAC 2 cut(s) 236, 661
HindIII AAGCTT 1 cut(s) 752
HinfI GANTC 3 cut(s) 79, 547, 578
HpaII CCGG 4 cut(s) 76, 367, 501, 509
HphI GGTGA 1 cut(s) 400
Hpy166II GTNNAC 2 cut(s) 236, 661
Hpy188I TCNGA 1 cut(s) 285
Hpy188III TCNNGA 7 cut(s) 90, 320, 326, 458, 575, 643, 740
Hpy8I GTNNAC 2 cut(s) 236, 661
Hpy99I CGWCG 2 cut(s) 34, 237
HpyAV CCTTC 1 cut(s) 398
HpyCH4IV ACGT 1 cut(s) 292
HpyCH4V TGCA 7 cut(s) 65, 104, 113, 585, 688, 758, 803
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 113
HpyF3I CTNAG 2 cut(s) 450, 794
HpySE526I ACGT 1 cut(s) 292
Hsp92I GRCGYC 2 cut(s) 32, 49
Hsp92II CATG 7 cut(s) 14, 65, 148, 247, 388, 585, 733
HspAI GCGC 1 cut(s) 219
Kzo9I GATC 6 cut(s) 322, 399, 465, 555, 736, 790
LmnI GCTCC 2 cut(s) 128, 407
Lsp1109I GCAGC 7 cut(s) 88, 116, 125, 128, 700, 815, 818
LweI GCATC 1 cut(s) 790
MaeI CTAG 2 cut(s) 216, 656
MaeII ACGT 1 cut(s) 292
MaeIII GTNAC 2 cut(s) 445, 504
MalI GATC 6 cut(s) 324, 401, 467, 557, 738, 792
MboI GATC 6 cut(s) 322, 399, 465, 555, 736, 790
MboII GAAGA 8 cut(s) 121, 145, 484, 612, 643, 746, 757, 800
MflI RGATCY 1 cut(s) 790
MluCI AATT 1 cut(s) 761
MnlI CCTC 5 cut(s) 149, 182, 291, 384, 650
MseI TTAA 2 cut(s) 372, 711
MslI CAYNNNNRTG 2 cut(s) 60, 590
MspA1I CMGCKG 2 cut(s) 119, 565
MspI CCGG 4 cut(s) 76, 367, 501, 509
MspR9I CCNGG 4 cut(s) 190, 368, 441, 502
MvaI CCWGG 2 cut(s) 190, 441
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 2 cut(s) 110, 113
NciI CCSGG 2 cut(s) 368, 502
NdeII GATC 6 cut(s) 322, 399, 465, 555, 736, 790
NlaIII CATG 7 cut(s) 14, 65, 148, 247, 388, 585, 733
NlaIV GGNNCC 3 cut(s) 124, 357, 409
NspI RCATGY 2 cut(s) 65, 148
OliI CACNNNNGTG 1 cut(s) 590
PciI ACATGT 1 cut(s) 144
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PfeI GAWTC 3 cut(s) 79, 547, 578
PflMI CCANNNNNTGG 1 cut(s) 698
PkrI GCNGC 8 cut(s) 103, 106, 115, 118, 567, 690, 805, 808
PscI ACATGT 1 cut(s) 144
Psp6I CCWGG 2 cut(s) 188, 439
PspEI GGTNACC 1 cut(s) 504
PspGI CCWGG 2 cut(s) 188, 439
PspN4I GGNNCC 3 cut(s) 124, 357, 409
PstI CTGCAG 1 cut(s) 106
PstNI CAGNNNCTG 1 cut(s) 119
PsuI RGATCY 1 cut(s) 790
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 2 cut(s) 342, 785
RsaNI GTAC 2 cut(s) 341, 784
RseI CAYNNNNRTG 2 cut(s) 60, 590
SalI GTCGAC 1 cut(s) 234
SaqAI TTAA 2 cut(s) 372, 711
SatI GCNGC 8 cut(s) 102, 105, 114, 117, 566, 689, 804, 807
Sau3AI GATC 6 cut(s) 322, 399, 465, 555, 736, 790
ScrFI CCNGG 4 cut(s) 190, 368, 441, 502
SetI ASST 7 cut(s) 121, 194, 295, 528, 642, 756, 831
SfaNI GCATC 1 cut(s) 790
SfcI CTRYAG 1 cut(s) 102
SmiMI CAYNNNNRTG 2 cut(s) 60, 590
Sse9I AATT 1 cut(s) 761
SsiI CCGC 2 cut(s) 169, 565
SspMI CTAG 2 cut(s) 216, 656
StyD4I CCNGG 4 cut(s) 188, 366, 439, 500
TaiI ACGT 1 cut(s) 295
TaqI TCGA 5 cut(s) 235, 402, 481, 574, 768
TasI AATT 1 cut(s) 761
TauI GCSGC 1 cut(s) 568
TfiI GAWTC 3 cut(s) 79, 547, 578
Tru1I TTAA 2 cut(s) 372, 711
Tru9I TTAA 2 cut(s) 372, 711
TseI GCWGC 7 cut(s) 101, 104, 113, 116, 688, 803, 806
TspDTI ATGAA 4 cut(s) 260, 373, 570, 764
Van91I CCANNNNNTGG 1 cut(s) 698
XapI RAATTY 1 cut(s) 761
XceI RCATGY 2 cut(s) 65, 148
XmiI GTMKAC 1 cut(s) 235
XspI CTAG 2 cut(s) 216, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.