Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006661

Physical Location & Seq

Genomic Coordinates Forward (+)

36572371 .. 36573426

Gene Structure 1056 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1273900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1056 bp

ATGGTGTGTAAGATCTTGAAGAAAGCCGCAATCGACATAAATCCAATGTCCATCAGCCGCTGTCACGGCATAAGGCGGCGCCACCGCCACAACTGCTCAGTTATAAAGGCTGCAGCTGCTGCTGCCTCCTCTATCGTGCTGACCCGCATTGGCTTCAATAAAGGATTTAAGTTCATTCAAAAAAGACAAACCCGAGAGGGCCAACCCGAATCCGAATTAACCAAAGAACGCCGTCCGCTCCCACCGCTGACTTCGCCGGAGAAGCGGACCGTGTTCCTTGATCTCGACGAGACACTGGTCCATTCCGTGACGGGCCCGCCGCCCAAGAAATTTGACTTCGTCGTACGGCCGAAGATTCGCGGCAAAATCATGACTTTCTACGTCGTGAAGCGTCCCGGCGTGGACGAGTTTTTGGAGAAATTAGGGAACAAGTACGAGGTGGTTGTGTTCACGGCCGGGTTGCGCGAGTACGCAACGCTGGTGCTAGACCGGCTGGACCGGAAGCGGGTGATATCACACCGTCTCTACAGGGACACGTGTACGGAGATAAATGGTAGGTTCGTGAAGGACTTATCCGGATTGGGGAGGGACCTGAGCCGACTGGTGATCGTCGACGACAATCCCAACGCGTACTGCTTCCAGCCGGAGAACGCGGTCCCAATCCGGCCGTTTGTGGACGACGTGGCGGACCGGGAGTTGAGGAGGGTGCTGGAGTTCTTCGAGGACGAGGAGACTCGCAGTTGTGACGACATTAGGGACGCCGTGAGGCGGTTTGTTGGATATCACGAGTCGATAACTTCATCATCTGATGACGACGACGGTGACGACGATGGTGATGGGTTGGAGTTGGAGTTGGAGTTGATGGACGCCACGTGTAAGCTTCCGGTGCTGATCGTTTCATCAAAAGCGCGGGAAATCACGAGTCGACGTAAATTTCAGCCTTTTGGAATTCCCATTTTGGAGTTTCGGACGAGGATGGTGGTGATGTTCTGTTTGTTTCTGCTACTTCTTCATGTAATGTGCTTTCGCAGAGCATCAGTCCACACTTTTTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

352

Amino Acids

40.37

Weight (kDa)

9.0

Isoelectric Point (pI)

50.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	90 - 252	4.9e-53	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29770 AT1G29780 AT5G45700
fragaria_vesca	FvH4_3g07100 FvH4_3g07110 FvH4_3g36710
malus_domestica	MD00G1007300.v1.1 MD00G1007500.v1.1 MD10G1273900.v1.1 MD10G1274000.v1.1
prunus_persica	Prupe.4G068500_v2.0.a1 Prupe.4G068600_v2.0.a1
pyrus_communis	pycom05g27270 pycom10g22870
rosa_chinensis	RchiOBHm_Chr5g0012001 RchiOBHm_Chr5g0012011
rosa_laevigata	RLG00000031878
rosa_multiflora	Rmu_co8217880.1_g000001
rosa_roxburghii	Rroxscaffold_1G00064580
rosa_rugosa	Rorug04G0460300 Rorug04G0460300
rosa_samantha	Rh5BG088100 Rh5BG088200 Rh5CG102000 Rh5DG088700
rosa_wichuraiana	Rw5G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	104
AccB1I	GGYRCC	1 cut(s)	78
AccBSI	CCGCTC	1 cut(s)	238
AccI	GTMKAC	2 cut(s)	612, 925
AccII	CGCG	5 cut(s)	360, 465, 629, 653, 910
AccIII	TCCGGA	1 cut(s)	575
AcoI	YGGCCR	3 cut(s)	347, 453, 665
AcsI	RAATTY	3 cut(s)	329, 932, 948
AcvI	CACGTG	2 cut(s)	537, 873
AcyI	GRCGYC	3 cut(s)	79, 759, 867
AfaI	GTAC	5 cut(s)	345, 434, 470, 541, 632
AfiI	CCNNNNNNNGG	3 cut(s)	505, 582, 768
AflIII	ACRYGT	4 cut(s)	534, 536, 627, 872
AgsI	TTSAA	3 cut(s)	19, 157, 179
AjiI	CACGTC	1 cut(s)	682
AluBI	AGCT	2 cut(s)	116, 880
AluI	AGCT	2 cut(s)	116, 880
Alw26I	GTCTC	3 cut(s)	284, 527, 725
AlwNI	CAGNNNCTG	2 cut(s)	60, 119
Ama87I	CYCGRG	1 cut(s)	192
Aor13HI	TCCGGA	1 cut(s)	575
AoxI	GGCC	5 cut(s)	199, 313, 347, 453, 665
ApaI	GGGCCC	1 cut(s)	317
ApeKI	GCWGC	5 cut(s)	110, 113, 116, 119, 122
ApoI	RAATTY	3 cut(s)	329, 932, 948
AspLEI	GCGC	3 cut(s)	81, 465, 910
AspS9I	GGNCC	9 cut(s)	199, 267, 298, 313, 314, 496, 589, 655, 688
AsuC2I	CCSGG	3 cut(s)	396, 457, 692
AsuHPI	GGTGA	5 cut(s)	520, 616, 833, 845, 994
AvaI	CYCGRG	1 cut(s)	192
AvaII	GGWCC	6 cut(s)	267, 298, 496, 589, 655, 688
BaeGI	GKGCMC	1 cut(s)	317
BanI	GGYRCC	1 cut(s)	78
BanII	GRGCYC	1 cut(s)	317
BauI	CACGAG	2 cut(s)	785, 919
BbrPI	CACGTG	2 cut(s)	537, 873
BbvI	GCAGC	5 cut(s)	97, 103, 106, 109, 125
BccI	CCATC	5 cut(s)	59, 824, 830, 856, 970
BceAI	ACGGC	6 cut(s)	82, 216, 362, 468, 652, 746
BcnI	CCSGG	3 cut(s)	396, 457, 692
BcoDI	GTCTC	3 cut(s)	284, 527, 725
BfaI	CTAG	1 cut(s)	485
BfmI	CTRYAG	2 cut(s)	111, 526
BfoI	RGCGCY	1 cut(s)	82
BglII	AGATCT	1 cut(s)	12
Bme1390I	CCNGG	3 cut(s)	396, 457, 692
Bme18I	GGWCC	6 cut(s)	267, 298, 496, 589, 655, 688
BmeT110I	CYCGRG	1 cut(s)	192
BmgBI	CACGTC	1 cut(s)	682
BmgT120I	GGNCC	9 cut(s)	199, 267, 298, 313, 314, 496, 589, 655, 688
BmiI	GGNNCC	4 cut(s)	80, 315, 590, 657
BmrFI	CCNGG	3 cut(s)	396, 457, 692
BmsI	GCATC	1 cut(s)	1043
BoxI	GACNNNNGTC	1 cut(s)	296
BpmI	CTGGAG	1 cut(s)	731
Bpu10I	CCTNAGC	1 cut(s)	593
BpuMI	CCSGG	3 cut(s)	396, 457, 692
BsaAI	YACGTR	2 cut(s)	537, 873
BsaHI	GRCGYC	3 cut(s)	79, 759, 867
BsaWI	WCCGGW	3 cut(s)	498, 575, 883
BsaXI	ACNNNNNCTCC	4 cut(s)	836, 842, 866, 872
Bsc4I	CCNNNNNNNGG	3 cut(s)	505, 582, 768
Bse118I	RCCGGY	1 cut(s)	489
Bse1I	ACTGG	2 cut(s)	300, 606
BseAI	TCCGGA	1 cut(s)	575
BseGI	GGATG	1 cut(s)	981
BseLI	CCNNNNNNNGG	3 cut(s)	505, 582, 768
BseMII	CTCAG	2 cut(s)	111, 584
BseNI	ACTGG	2 cut(s)	300, 606
BseRI	GAGGAG	3 cut(s)	118, 715, 743
BseSI	GKGCMC	1 cut(s)	317
BseX3I	CGGCCG	3 cut(s)	347, 453, 665
BseXI	GCAGC	5 cut(s)	97, 103, 106, 109, 125
Bsh1236I	CGCG	5 cut(s)	360, 465, 629, 653, 910
Bsh1285I	CGRYCG	3 cut(s)	350, 456, 668
BshFI	GGCC	5 cut(s)	201, 315, 349, 455, 667
BshNI	GGYRCC	1 cut(s)	78
BsiEI	CGRYCG	3 cut(s)	350, 456, 668
BsiHKCI	CYCGRG	1 cut(s)	192
BsiWI	CGTACG	1 cut(s)	343
BslFI	GGGAC	5 cut(s)	378, 545, 602, 641, 770
BslI	CCNNNNNNNGG	3 cut(s)	505, 582, 768
BsmAI	GTCTC	3 cut(s)	284, 527, 725
BsmBI	CGTCTC	1 cut(s)	527
BsmFI	GGGAC	5 cut(s)	378, 545, 602, 641, 770
BsnI	GGCC	5 cut(s)	201, 315, 349, 455, 667
BsoBI	CYCGRG	1 cut(s)	192
Bsp120I	GGGCCC	1 cut(s)	313
Bsp1286I	GDGCHC	1 cut(s)	317
Bsp13I	TCCGGA	1 cut(s)	575
Bsp143I	GATC	4 cut(s)	12, 280, 606, 891
BspANI	GGCC	5 cut(s)	201, 315, 349, 455, 667
BspCNI	CTCAG	2 cut(s)	110, 585
BspEI	TCCGGA	1 cut(s)	575
BspFNI	CGCG	5 cut(s)	360, 465, 629, 653, 910
BspHI	TCATGA	1 cut(s)	369
BspLI	GGNNCC	4 cut(s)	80, 315, 590, 657
BspMAI	CTGCAG	1 cut(s)	115
BspT107I	GGYRCC	1 cut(s)	78
BsrBI	CCGCTC	1 cut(s)	238
BsrFI	RCCGGY	1 cut(s)	489
BsrI	ACTGG	2 cut(s)	300, 606
BssAI	RCCGGY	1 cut(s)	489
BssMI	GATC	4 cut(s)	12, 280, 606, 891
BssNI	GRCGYC	3 cut(s)	79, 759, 867
BssSI	CACGAG	2 cut(s)	785, 919
Bst2BI	CACGAG	2 cut(s)	785, 919
Bst4CI	ACNGT	3 cut(s)	271, 521, 821
BstACI	GRCGYC	3 cut(s)	79, 759, 867
BstAPI	GCANNNNNTGC	1 cut(s)	119
BstBAI	YACGTR	2 cut(s)	537, 873
BstC8I	GCNNGC	1 cut(s)	317
BstDEI	CTNAG	2 cut(s)	97, 593
BstF5I	GGATG	1 cut(s)	981
BstFNI	CGCG	5 cut(s)	360, 465, 629, 653, 910
BstH2I	RGCGCY	1 cut(s)	82
BstHHI	GCGC	3 cut(s)	81, 465, 910
BstKTI	GATC	4 cut(s)	15, 283, 609, 894
BstMAI	GTCTC	3 cut(s)	284, 527, 725
BstMBI	GATC	4 cut(s)	12, 280, 606, 891
BstMCI	CGRYCG	3 cut(s)	350, 456, 668
BstPAI	GACNNNNGTC	1 cut(s)	296
BstSCI	CCNGG	3 cut(s)	394, 455, 690
BstSFI	CTRYAG	2 cut(s)	111, 526
BstSLI	GKGCMC	1 cut(s)	317
BstUI	CGCG	5 cut(s)	360, 465, 629, 653, 910
BstV1I	GCAGC	5 cut(s)	97, 103, 106, 109, 125
BstX2I	RGATCY	1 cut(s)	12
BstYI	RGATCY	1 cut(s)	12
BstZI	CGGCCG	3 cut(s)	347, 453, 665
BsuRI	GGCC	5 cut(s)	201, 315, 349, 455, 667
BtrI	CACGTC	1 cut(s)	682
BtsCI	GGATG	1 cut(s)	981
BtsIMutI	CAGTG	1 cut(s)	293
Cac8I	GCNNGC	1 cut(s)	317
CaiI	CAGNNNCTG	2 cut(s)	60, 119
CciI	TCATGA	1 cut(s)	369
CfoI	GCGC	3 cut(s)	81, 465, 910
Cfr10I	RCCGGY	1 cut(s)	489
Cfr13I	GGNCC	9 cut(s)	199, 267, 298, 313, 314, 496, 589, 655, 688
CpoI	CGGWCCG	2 cut(s)	267, 688
CseI	GACGC	3 cut(s)	380, 767, 875
Csp6I	GTAC	5 cut(s)	344, 433, 469, 540, 631
CspI	CGGWCCG	2 cut(s)	267, 688
CviAII	CATG	2 cut(s)	370, 1013
CviQI	GTAC	5 cut(s)	344, 433, 469, 540, 631
DdeI	CTNAG	2 cut(s)	97, 593
DinI	GGCGCC	1 cut(s)	80
DpnI	GATC	4 cut(s)	14, 282, 608, 893
DpnII	GATC	4 cut(s)	12, 280, 606, 891
EaeI	YGGCCR	3 cut(s)	347, 453, 665
EagI	CGGCCG	3 cut(s)	347, 453, 665
EciI	GGCGGA	1 cut(s)	701
EclXI	CGGCCG	3 cut(s)	347, 453, 665
Eco24I	GRGCYC	1 cut(s)	317
Eco32I	GATATC	2 cut(s)	513, 782
Eco47I	GGWCC	6 cut(s)	267, 298, 496, 589, 655, 688
Eco52I	CGGCCG	3 cut(s)	347, 453, 665
Eco72I	CACGTG	2 cut(s)	537, 873
Eco88I	CYCGRG	1 cut(s)	192
EcoO109I	RGGNCCY	1 cut(s)	589
EcoRI	GAATTC	1 cut(s)	948
EcoRV	GATATC	2 cut(s)	513, 782
EcoT38I	GRGCYC	1 cut(s)	317
EgeI	GGCGCC	1 cut(s)	80
EheI	GGCGCC	1 cut(s)	80
Esp3I	CGTCTC	1 cut(s)	527
FaeI	CATG	2 cut(s)	373, 1016
FaiI	YATR	5 cut(s)	38, 71, 104, 371, 1014
FaqI	GGGAC	5 cut(s)	378, 545, 602, 641, 770
FatI	CATG	2 cut(s)	369, 1012
FauI	CCCGC	4 cut(s)	152, 324, 498, 903
FblI	GTMKAC	2 cut(s)	612, 925
FokI	GGATG	1 cut(s)	988
FriOI	GRGCYC	1 cut(s)	317
FspBI	CTAG	1 cut(s)	485
GlaI	GCGC	3 cut(s)	80, 464, 909
GsuI	CTGGAG	1 cut(s)	731
HaeII	RGCGCY	1 cut(s)	82
HaeIII	GGCC	5 cut(s)	201, 315, 349, 455, 667
HgaI	GACGC	3 cut(s)	380, 767, 875
HhaI	GCGC	3 cut(s)	81, 465, 910
Hin1I	GRCGYC	3 cut(s)	79, 759, 867
Hin1II	CATG	2 cut(s)	373, 1016
Hin6I	GCGC	3 cut(s)	79, 463, 908
HinP1I	GCGC	3 cut(s)	79, 463, 908
HincII	GTYRAC	2 cut(s)	613, 926
HindII	GTYRAC	2 cut(s)	613, 926
HindIII	AAGCTT	1 cut(s)	878
HinfI	GANTC	5 cut(s)	209, 355, 733, 788, 922
HphI	GGTGA	5 cut(s)	520, 616, 833, 845, 994
Hpy166II	GTNNAC	7 cut(s)	403, 450, 540, 613, 676, 926, 1042
Hpy188I	TCNGA	3 cut(s)	214, 808, 969
Hpy188III	TCNNGA	8 cut(s)	16, 284, 370, 385, 562, 576, 785, 919
Hpy8I	GTNNAC	7 cut(s)	403, 450, 540, 613, 676, 926, 1042
HpyAV	CCTTC	1 cut(s)	559
HpyCH4III	ACNGT	3 cut(s)	271, 521, 821
HpyCH4IV	ACGT	5 cut(s)	381, 536, 681, 872, 928
HpyCH4V	TGCA	1 cut(s)	113
HpyF3I	CTNAG	2 cut(s)	97, 593
HpySE526I	ACGT	5 cut(s)	381, 536, 681, 872, 928
Hsp92I	GRCGYC	3 cut(s)	79, 759, 867
Hsp92II	CATG	2 cut(s)	373, 1016
HspAI	GCGC	3 cut(s)	79, 463, 908
KasI	GGCGCC	1 cut(s)	78
Kpn2I	TCCGGA	1 cut(s)	575
Kzo9I	GATC	4 cut(s)	12, 280, 606, 891
LmnI	GCTCC	1 cut(s)	243
Lsp1109I	GCAGC	5 cut(s)	97, 103, 106, 109, 125
LweI	GCATC	1 cut(s)	1043
MaeI	CTAG	1 cut(s)	485
MaeII	ACGT	5 cut(s)	381, 536, 681, 872, 928
MaeIII	GTNAC	4 cut(s)	62, 307, 743, 821
MalI	GATC	4 cut(s)	14, 282, 608, 893
MbiI	CCGCTC	1 cut(s)	238
MboI	GATC	4 cut(s)	12, 280, 606, 891
MboII	GAAGA	4 cut(s)	31, 364, 709, 1001
MflI	RGATCY	1 cut(s)	12
MhlI	GDGCHC	1 cut(s)	317
MluCI	AATT	5 cut(s)	215, 329, 419, 932, 948
MluI	ACGCGT	1 cut(s)	627
Mly113I	GGCGCC	1 cut(s)	79
MlyI	GAGTC	3 cut(s)	727, 797, 931
MmeI	TCCRAC	4 cut(s)	757, 822, 828, 834
MroI	TCCGGA	1 cut(s)	575
MseI	TTAA	2 cut(s)	168, 218
MspA1I	CMGCKG	3 cut(s)	60, 116, 247
MspR9I	CCNGG	3 cut(s)	396, 457, 692
MvnI	CGCG	5 cut(s)	360, 465, 629, 653, 910
NarI	GGCGCC	1 cut(s)	79
NciI	CCSGG	3 cut(s)	396, 457, 692
NdeII	GATC	4 cut(s)	12, 280, 606, 891
NlaIII	CATG	2 cut(s)	373, 1016
NlaIV	GGNNCC	4 cut(s)	80, 315, 590, 657
NmuCI	GTSAC	4 cut(s)	62, 307, 743, 821
PagI	TCATGA	1 cut(s)	369
PcsI	WCGNNNNNNNCGW	2 cut(s)	822, 825
PfeI	GAWTC	2 cut(s)	209, 355
Pfl23II	CGTACG	1 cut(s)	343
PflFI	GACNNNGTC	1 cut(s)	338
PleI	GAGTC	3 cut(s)	727, 796, 930
PluTI	GGCGCC	1 cut(s)	82
PmaCI	CACGTG	2 cut(s)	537, 873
PmlI	CACGTG	2 cut(s)	537, 873
PpsI	GAGTC	3 cut(s)	727, 796, 930
Ppu21I	YACGTR	2 cut(s)	537, 873
PpuMI	RGGWCCY	1 cut(s)	589
PshAI	GACNNNNGTC	1 cut(s)	296
PsiI	TTATAA	1 cut(s)	104
Psp5II	RGGWCCY	1 cut(s)	589
PspCI	CACGTG	2 cut(s)	537, 873
PspLI	CGTACG	1 cut(s)	343
PspN4I	GGNNCC	4 cut(s)	80, 315, 590, 657
PspOMI	GGGCCC	1 cut(s)	313
PspPI	GGNCC	9 cut(s)	199, 267, 298, 313, 314, 496, 589, 655, 688
PspPPI	RGGWCCY	1 cut(s)	589
PstI	CTGCAG	1 cut(s)	115
PstNI	CAGNNNCTG	2 cut(s)	60, 119
PsuI	RGATCY	1 cut(s)	12
PsyI	GACNNNGTC	1 cut(s)	338
PvuII	CAGCTG	1 cut(s)	116
RsaI	GTAC	5 cut(s)	345, 434, 470, 541, 632
RsaNI	GTAC	5 cut(s)	344, 433, 469, 540, 631
Rsr2I	CGGWCCG	2 cut(s)	267, 688
RsrII	CGGWCCG	2 cut(s)	267, 688
SalI	GTCGAC	2 cut(s)	611, 924
SaqAI	TTAA	2 cut(s)	168, 218
Sau3AI	GATC	4 cut(s)	12, 280, 606, 891
Sau96I	GGNCC	9 cut(s)	199, 267, 298, 313, 314, 496, 589, 655, 688
SchI	GAGTC	3 cut(s)	727, 797, 931
ScrFI	CCNGG	3 cut(s)	396, 457, 692
SduI	GDGCHC	1 cut(s)	317
SfaNI	GCATC	1 cut(s)	1043
SfcI	CTRYAG	2 cut(s)	111, 526
SfoI	GGCGCC	1 cut(s)	80
SgrDI	CGTCGACG	1 cut(s)	611
SinI	GGWCC	6 cut(s)	267, 298, 496, 589, 655, 688
Sse9I	AATT	5 cut(s)	215, 329, 419, 932, 948
SspDI	GGCGCC	1 cut(s)	78
SspMI	CTAG	1 cut(s)	485
StyD4I	CCNGG	3 cut(s)	394, 455, 690
TaaI	ACNGT	3 cut(s)	271, 521, 821
TaiI	ACGT	5 cut(s)	384, 539, 684, 875, 931
TaqI	TCGA	6 cut(s)	33, 285, 612, 720, 791, 925
TasI	AATT	5 cut(s)	215, 329, 419, 932, 948
TauI	GCSGC	5 cut(s)	29, 60, 79, 322, 363
TfiI	GAWTC	2 cut(s)	209, 355
Tru1I	TTAA	2 cut(s)	168, 218
Tru9I	TTAA	2 cut(s)	168, 218
TscAI	CASTG	1 cut(s)	300
TseFI	GTSAC	4 cut(s)	62, 307, 743, 821
TseI	GCWGC	5 cut(s)	110, 113, 116, 119, 122
Tsp45I	GTSAC	4 cut(s)	62, 307, 743, 821
TspDTI	ATGAA	4 cut(s)	163, 789, 888, 1001
TspGWI	ACGGA	2 cut(s)	295, 557
TspRI	CASTG	1 cut(s)	300
Tth111I	GACNNNGTC	1 cut(s)	338
VpaK11BI	GGWCC	6 cut(s)	267, 298, 496, 589, 655, 688
XapI	RAATTY	3 cut(s)	329, 932, 948
XmiI	GTMKAC	2 cut(s)	612, 925
XspI	CTAG	1 cut(s)	485

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.