Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0006661

Physical Location & Seq

Genomic Coordinates Forward (+)

25169888 .. 25173508

Gene Structure 3621 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g22870.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1794 bp

ATGGTGTGCAAGATCTTGAAGAAAACCGCAATCGACATAAATCCCATGTCCATCAACCGCTGTCGCGGCATAAGGCGGCGCCACCGCCACAACTGCTCAGTTATAAAGGCTGCAGCCGCTGCCTCCTCTATCGTGCTGACCCGCATTGGCTTCAATAAAGGATTTAAGTTCATTGAAAAAAGACAAACCCGAGAGGGCCAACCCGAATCCGAATTAACCAAAGAACGCCGTCCGCTCCCACCGCTGACTTCGCCGGAGAAGCGGATCGTGTTCCTTGATCTCGACGAGACACTGGTCCATTCCGTGACGGGCCCGCCGCCCAAGAAATTTGACTTCGTCGTACGGCCGAAGATTCGCGGCAAAATCATGACTTTCTACGTCGTGAAGCGTCCCGGCGTAGACGAGTTTTTGGAGAAATTAGGGAACAAGTACGAGGTGGTTGTGTTCACGGCGGGGTTGCGCGAGTACGCAACGCTGGTGCTAGACCGGCTGGACCGGAAGCGGGTGATATCACACCGTCTCTACAGGGACACGTGTACGGAGGTAAATGGTAGGTTCGTGAAGGACTTATCCGGATTGGGGAGGGACCTGAGCCGACTGGTGATCGTCGACGACAATCCCAACGCGTACTGCTTCCAGCCGGAGAACGCGGTCCCAATCCGGCCGTTTGTGGACGACGTGGCGGACCGGGAGTTGGGGAGGGTGCTGGAGTTCTTCGAGGACGAGGAGACTCGCAGTTGTGACGACATTAGGGACGCCGTGAGGCGGTTTGTTGGATATGACGAGTCGATAACTTCATCATCTGATGACGAAGATGGTGACGACGGTGACGACGATGGTGATGGGTTGGAGTTGATGGACGCCACGTTTCTCTCATCAAGATTTCGCAGAAGTAATTGGGAAAGCAAGAAATTAAATTTTGAGGAAATGGTGTCCAAGATTGTGAAGAAGAATGCCAACAGGCGACGCAATCGCGGAAAGTCCTCGGCCAGGAAGGAGGCAGTCGCGGCCTCCTCCGCCGTGCTAGCCTCCCTCTTCGATTGCCCCCGCCGCCTACTCAAGCTCTTCGCGAAACTTGCGAGAATCGGCACGCCGAACCGCCACAGGGGCTTCAAGATCCTCAAGAAAGCCCAAGTCCACGAGGCGGAGACAGAATCTGAACCCGGATCGGGAAACCCGCTCTGCCGCAAGCTCTTGTTCGAGCCACCGCTTATGCCGCTGGTCTCTCCGGAGAAGATGACGGTGTTTCTCGACCTGGATGAGACCTTGGTGCACGCACAGGCCGACCCGCCGCCGGAGAAGTTCGATTTCATAGTGCGTCCGAGGATCGACGGAATCGAGATGAACTTTTATGTTCGTAAGCGTCCCGGAGTGGACGAGTTGTTGGAGAAGTTAGGGGCGAAGTACGAGGTGGTGGTATTCACGGCGGGGTTGAGAGAGTACGCGTCACTGGTGCTGGACCGGTTGGACCAGAAACGGGTGATTTCGCACCGGCTCTATCGAGACGCCTGTAAAGAAGTGGACGGGAAGTTCGTGAAGGACTTGTCCGGGTTGGGGAGGGACTTGAGCCGGGTGGTGATTGTGGACGACAATCCCAATTCGTACGTCTTTCAACCGGAGAATGCCGTTCCGGTCCGGCAGTTTGTGGATGACATGGAGGACCGGGAGCTGGGGAGGTTGGTGGAGTTCTTTGAAGGGTTGGATTGTGAAGATATGAGGGACGCTGTGAAGAAATTTCTCGGCAGCACTGGCGGCAGAGACGGCGGTGGATCAAAAGACGGCAAAGCAGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

598

Amino Acids

67.84

Weight (kDa)

8.7

Isoelectric Point (pI)

42.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	89 - 251	4e-52	NLI interacting factor-like phosphatase
NIF	PF03031	414 - 568	5.3e-52	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29770 AT1G29780 AT5G45700
fragaria_vesca	FvH4_3g07100 FvH4_3g07110 FvH4_3g36710
malus_domestica	MD00G1007300.v1.1 MD00G1007500.v1.1 MD10G1273900.v1.1 MD10G1274000.v1.1
prunus_persica	Prupe.4G068500_v2.0.a1 Prupe.4G068600_v2.0.a1
pyrus_communis	pycom05g27270 pycom10g22870
rosa_chinensis	RchiOBHm_Chr5g0012001 RchiOBHm_Chr5g0012011
rosa_laevigata	RLG00000031878
rosa_multiflora	Rmu_co8217880.1_g000001
rosa_roxburghii	Rroxscaffold_1G00064580
rosa_rugosa	Rorug04G0460300 Rorug04G0460300
rosa_samantha	Rh5BG088100 Rh5BG088200 Rh5CG102000 Rh5DG088700
rosa_wichuraiana	Rw5G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	104
AccB1I	GGYRCC	1 cut(s)	78
AccBSI	CCGCTC	2 cut(s)	235, 1180
AccI	GTMKAC	2 cut(s)	399, 609
AccII	CGCG	9 cut(s)	66, 357, 462, 626, 650, 975, 1007, 1070, 1445
AccIII	TCCGGA	2 cut(s)	572, 1228
AclWI	GGATC	5 cut(s)	272, 1111, 1174, 1334, 1777
AcoI	YGGCCR	3 cut(s)	344, 662, 987
AcsI	RAATTY	3 cut(s)	326, 916, 1733
AcvI	CACGTG	1 cut(s)	534
AcyI	GRCGYC	4 cut(s)	79, 756, 861, 1506
AfaI	GTAC	8 cut(s)	342, 431, 467, 538, 629, 1406, 1442, 1604
AflIII	ACRYGT	4 cut(s)	531, 533, 624, 1443
AgeI	ACCGGT	1 cut(s)	1461
AgsI	TTSAA	6 cut(s)	19, 154, 176, 1114, 1613, 1694
AjiI	CACGTC	1 cut(s)	679
AjnI	CCWGG	2 cut(s)	989, 1254
AloI	GAACNNNNNNTCC	2 cut(s)	1514, 1546
AluBI	AGCT	3 cut(s)	1063, 1192, 1669
AluI	AGCT	3 cut(s)	1063, 1192, 1669
Alw21I	GWGCWC	1 cut(s)	1275
Alw26I	GTCTC	8 cut(s)	281, 524, 722, 1142, 1228, 1256, 1497, 1752
Alw44I	GTGCAC	1 cut(s)	1271
AlwI	GGATC	5 cut(s)	272, 1111, 1174, 1334, 1777
AlwNI	CAGNNNCTG	2 cut(s)	119, 1157
Ama87I	CYCGRG	1 cut(s)	189
Aor13HI	TCCGGA	2 cut(s)	572, 1228
AoxI	GGCC	7 cut(s)	196, 310, 344, 662, 987, 1008, 1281
ApaI	GGGCCC	1 cut(s)	314
ApaLI	GTGCAC	1 cut(s)	1271
ApeKI	GCWGC	4 cut(s)	110, 113, 119, 1743
ApoI	RAATTY	3 cut(s)	326, 916, 1733
ArsI	GACNNNNNNTTYG	2 cut(s)	1514, 1546
AsiGI	ACCGGT	1 cut(s)	1461
AspLEI	GCGC	2 cut(s)	81, 462
AsuC2I	CCSGG	7 cut(s)	393, 689, 1164, 1368, 1549, 1571, 1664
AsuHPI	GGTGA	7 cut(s)	517, 613, 830, 839, 851, 1492, 1588
AsuNHI	GCTAGC	1 cut(s)	1024
AvaI	CYCGRG	1 cut(s)	189
AvaII	GGWCC	9 cut(s)	295, 493, 586, 652, 685, 1459, 1468, 1633, 1660
BaeGI	GKGCMC	2 cut(s)	314, 1275
BanI	GGYRCC	1 cut(s)	78
BanII	GRGCYC	1 cut(s)	314
BauI	CACGAG	1 cut(s)	1139
BbrPI	CACGTG	1 cut(s)	534
Bbv12I	GWGCWC	1 cut(s)	1275
BbvI	GCAGC	4 cut(s)	97, 106, 125, 1755
BccI	CCATC	5 cut(s)	59, 809, 830, 836, 850
BceAI	ACGGC	9 cut(s)	213, 359, 465, 649, 743, 1004, 1440, 1610, 1777
BciT130I	CCWGG	2 cut(s)	991, 1256
BcnI	CCSGG	7 cut(s)	393, 689, 1164, 1368, 1549, 1571, 1664
BcoDI	GTCTC	8 cut(s)	281, 524, 722, 1142, 1228, 1256, 1497, 1752
BfaI	CTAG	2 cut(s)	482, 1025
BfmI	CTRYAG	2 cut(s)	111, 523
BfoI	RGCGCY	1 cut(s)	82
BglI	GCCNNNNNGGC	1 cut(s)	1107
BglII	AGATCT	1 cut(s)	12
Bme1390I	CCNGG	9 cut(s)	393, 689, 991, 1164, 1256, 1368, 1549, 1571, 1664
Bme18I	GGWCC	9 cut(s)	295, 493, 586, 652, 685, 1459, 1468, 1633, 1660
BmeT110I	CYCGRG	1 cut(s)	189
BmgBI	CACGTC	1 cut(s)	679
BmiI	GGNNCC	4 cut(s)	80, 312, 587, 654
BmrFI	CCNGG	9 cut(s)	393, 689, 991, 1164, 1256, 1368, 1549, 1571, 1664
BmtI	GCTAGC	1 cut(s)	1028
BoxI	GACNNNNGTC	1 cut(s)	293
BpmI	CTGGAG	1 cut(s)	728
Bpu10I	CCTNAGC	1 cut(s)	590
BpuEI	CTTGAG	3 cut(s)	1043, 1106, 1585
BpuMI	CCSGG	7 cut(s)	393, 689, 1164, 1368, 1549, 1571, 1664
BsaAI	YACGTR	1 cut(s)	534
BsaHI	GRCGYC	4 cut(s)	79, 756, 861, 1506
BsaI	GGTCTC	2 cut(s)	1228, 1256
BsaJI	CCNNGG	3 cut(s)	984, 1266, 1322
BsaWI	WCCGGW	6 cut(s)	495, 572, 1228, 1461, 1615, 1630
Bse118I	RCCGGY	3 cut(s)	486, 1461, 1491
Bse1I	ACTGG	4 cut(s)	297, 603, 1455, 1753
BseAI	TCCGGA	2 cut(s)	572, 1228
BseBI	CCWGG	2 cut(s)	991, 1256
BseDI	CCNNGG	3 cut(s)	984, 1266, 1322
BseGI	GGATG	2 cut(s)	1264, 1654
BseMII	CTCAG	2 cut(s)	111, 581
BseNI	ACTGG	4 cut(s)	297, 603, 1455, 1753
BseRI	GAGGAG	3 cut(s)	115, 740, 1003
BseSI	GKGCMC	2 cut(s)	314, 1275
BseX3I	CGGCCG	2 cut(s)	344, 662
BseXI	GCAGC	4 cut(s)	97, 106, 125, 1755
BseYI	CCCAGC	1 cut(s)	1669
Bsh1236I	CGCG	9 cut(s)	66, 357, 462, 626, 650, 975, 1007, 1070, 1445
Bsh1285I	CGRYCG	2 cut(s)	347, 665
BshFI	GGCC	7 cut(s)	198, 312, 346, 664, 989, 1010, 1283
BshNI	GGYRCC	1 cut(s)	78
BshTI	ACCGGT	1 cut(s)	1461
BsiEI	CGRYCG	2 cut(s)	347, 665
BsiHKAI	GWGCWC	1 cut(s)	1275
BsiHKCI	CYCGRG	1 cut(s)	189
BsiWI	CGTACG	2 cut(s)	340, 1602
BslFI	GGGAC	8 cut(s)	375, 542, 599, 638, 767, 1350, 1574, 1733
BsmAI	GTCTC	8 cut(s)	281, 524, 722, 1142, 1228, 1256, 1497, 1752
BsmBI	CGTCTC	3 cut(s)	524, 1497, 1752
BsmFI	GGGAC	8 cut(s)	375, 542, 599, 638, 767, 1350, 1574, 1733
BsmI	GAATGC	2 cut(s)	958, 1627
BsnI	GGCC	7 cut(s)	198, 312, 346, 664, 989, 1010, 1283
Bso31I	GGTCTC	2 cut(s)	1228, 1256
BsoBI	CYCGRG	1 cut(s)	189
Bsp120I	GGGCCC	1 cut(s)	310
Bsp1286I	GDGCHC	2 cut(s)	314, 1275
Bsp13I	TCCGGA	2 cut(s)	572, 1228
Bsp143I	GATC	8 cut(s)	12, 264, 277, 603, 1116, 1166, 1326, 1769
Bsp68I	TCGCGA	1 cut(s)	1070
BspANI	GGCC	7 cut(s)	198, 312, 346, 664, 989, 1010, 1283
BspCNI	CTCAG	2 cut(s)	110, 582
BspEI	TCCGGA	2 cut(s)	572, 1228
BspFNI	CGCG	9 cut(s)	66, 357, 462, 626, 650, 975, 1007, 1070, 1445
BspHI	TCATGA	1 cut(s)	366
BspLI	GGNNCC	4 cut(s)	80, 312, 587, 654
BspMAI	CTGCAG	1 cut(s)	115
BspOI	GCTAGC	1 cut(s)	1028
BspPI	GGATC	5 cut(s)	272, 1111, 1174, 1334, 1777
BspQI	GCTCTTC	1 cut(s)	1070
BspT107I	GGYRCC	1 cut(s)	78
BspTNI	GGTCTC	2 cut(s)	1228, 1256
BsrBI	CCGCTC	2 cut(s)	235, 1180
BsrFI	RCCGGY	3 cut(s)	486, 1461, 1491
BsrI	ACTGG	4 cut(s)	297, 603, 1455, 1753
BssAI	RCCGGY	3 cut(s)	486, 1461, 1491
BssECI	CCNNGG	3 cut(s)	984, 1266, 1322
BssMI	GATC	8 cut(s)	12, 264, 277, 603, 1116, 1166, 1326, 1769
BssNI	GRCGYC	4 cut(s)	79, 756, 861, 1506
BssSI	CACGAG	1 cut(s)	1139
BssT1I	CCWWGG	1 cut(s)	1266
Bst2BI	CACGAG	1 cut(s)	1139
Bst2UI	CCWGG	2 cut(s)	991, 1256
Bst4CI	ACNGT	3 cut(s)	518, 827, 1243
Bst6I	CTCTTC	2 cut(s)	1040, 1070
BstACI	GRCGYC	4 cut(s)	79, 756, 861, 1506
BstAPI	GCANNNNNTGC	1 cut(s)	119
BstBAI	YACGTR	1 cut(s)	534
BstC8I	GCNNGC	5 cut(s)	314, 1026, 1091, 1190, 1275
BstDEI	CTNAG	2 cut(s)	97, 590
BstENI	CCTNNNNNAGG	1 cut(s)	988
BstF5I	GGATG	2 cut(s)	1264, 1654
BstFNI	CGCG	9 cut(s)	66, 357, 462, 626, 650, 975, 1007, 1070, 1445
BstH2I	RGCGCY	1 cut(s)	82
BstHHI	GCGC	2 cut(s)	81, 462
BstKTI	GATC	8 cut(s)	15, 267, 280, 606, 1119, 1169, 1329, 1772
BstMAI	GTCTC	8 cut(s)	281, 524, 722, 1142, 1228, 1256, 1497, 1752
BstMBI	GATC	8 cut(s)	12, 264, 277, 603, 1116, 1166, 1326, 1769
BstMCI	CGRYCG	2 cut(s)	347, 665
BstNI	CCWGG	2 cut(s)	991, 1256
BstPAI	GACNNNNGTC	1 cut(s)	293
BstSCI	CCNGG	9 cut(s)	391, 687, 989, 1162, 1254, 1366, 1547, 1569, 1662
BstSFI	CTRYAG	2 cut(s)	111, 523
BstSLI	GKGCMC	2 cut(s)	314, 1275
BstUI	CGCG	9 cut(s)	66, 357, 462, 626, 650, 975, 1007, 1070, 1445
BstV1I	GCAGC	4 cut(s)	97, 106, 125, 1755
BstX2I	RGATCY	2 cut(s)	12, 1116
BstYI	RGATCY	2 cut(s)	12, 1116
BstZI	CGGCCG	2 cut(s)	344, 662
BsuRI	GGCC	7 cut(s)	198, 312, 346, 664, 989, 1010, 1283
BtrI	CACGTC	1 cut(s)	679
BtsCI	GGATG	2 cut(s)	1264, 1654
BtsI	GCAGTG	1 cut(s)	1794
BtsIMutI	CAGTG	4 cut(s)	290, 1448, 1746, 1794
BtuMI	TCGCGA	1 cut(s)	1070
Cac8I	GCNNGC	5 cut(s)	314, 1026, 1091, 1190, 1275
CaiI	CAGNNNCTG	2 cut(s)	119, 1157
CciI	TCATGA	1 cut(s)	366
CfoI	GCGC	2 cut(s)	81, 462
Cfr10I	RCCGGY	3 cut(s)	486, 1461, 1491
CpoI	CGGWCCG	2 cut(s)	685, 1633
CseI	GACGC	9 cut(s)	377, 764, 869, 975, 1307, 1352, 1434, 1514, 1730
Csp6I	GTAC	8 cut(s)	341, 430, 466, 537, 628, 1405, 1441, 1603
CspAI	ACCGGT	1 cut(s)	1461
CspI	CGGWCCG	2 cut(s)	685, 1633
CviAII	CATG	3 cut(s)	46, 367, 1654
CviQI	GTAC	8 cut(s)	341, 430, 466, 537, 628, 1405, 1441, 1603
DdeI	CTNAG	2 cut(s)	97, 590
DinI	GGCGCC	1 cut(s)	80
DpnI	GATC	8 cut(s)	14, 266, 279, 605, 1118, 1168, 1328, 1771
DpnII	GATC	8 cut(s)	12, 264, 277, 603, 1116, 1166, 1326, 1769
EaeI	YGGCCR	3 cut(s)	344, 662, 987
EagI	CGGCCG	2 cut(s)	344, 662
Eam1104I	CTCTTC	2 cut(s)	1040, 1070
EarI	CTCTTC	2 cut(s)	1040, 1070
EciI	GGCGGA	3 cut(s)	698, 1006, 1160
EclXI	CGGCCG	2 cut(s)	344, 662
Eco130I	CCWWGG	1 cut(s)	1266
Eco24I	GRGCYC	1 cut(s)	314
Eco31I	GGTCTC	2 cut(s)	1228, 1256
Eco32I	GATATC	1 cut(s)	510
Eco47I	GGWCC	9 cut(s)	295, 493, 586, 652, 685, 1459, 1468, 1633, 1660
Eco52I	CGGCCG	2 cut(s)	344, 662
Eco72I	CACGTG	1 cut(s)	534
Eco88I	CYCGRG	1 cut(s)	189
EcoNI	CCTNNNNNAGG	1 cut(s)	988
EcoO109I	RGGNCCY	1 cut(s)	586
EcoRII	CCWGG	2 cut(s)	989, 1254
EcoRV	GATATC	1 cut(s)	510
EcoT14I	CCWWGG	1 cut(s)	1266
EcoT38I	GRGCYC	1 cut(s)	314
EgeI	GGCGCC	1 cut(s)	80
EheI	GGCGCC	1 cut(s)	80
ErhI	CCWWGG	1 cut(s)	1266
Esp3I	CGTCTC	3 cut(s)	524, 1497, 1752
FaeI	CATG	3 cut(s)	49, 370, 1657
FaqI	GGGAC	8 cut(s)	375, 542, 599, 638, 767, 1350, 1574, 1733
FatI	CATG	3 cut(s)	45, 366, 1653
FauI	CCCGC	8 cut(s)	149, 321, 445, 495, 1055, 1185, 1296, 1420
FblI	GTMKAC	2 cut(s)	399, 609
FokI	GGATG	2 cut(s)	1271, 1661
FriOI	GRGCYC	1 cut(s)	314
FspBI	CTAG	2 cut(s)	482, 1025
GlaI	GCGC	2 cut(s)	80, 461
GsaI	CCCAGC	1 cut(s)	1673
GsuI	CTGGAG	1 cut(s)	728
HaeII	RGCGCY	1 cut(s)	82
HaeIII	GGCC	7 cut(s)	198, 312, 346, 664, 989, 1010, 1283
HgaI	GACGC	9 cut(s)	377, 764, 869, 975, 1307, 1352, 1434, 1514, 1730
HhaI	GCGC	2 cut(s)	81, 462
Hin1I	GRCGYC	4 cut(s)	79, 756, 861, 1506
Hin1II	CATG	3 cut(s)	49, 370, 1657
Hin6I	GCGC	2 cut(s)	79, 460
HinP1I	GCGC	2 cut(s)	79, 460
HincII	GTYRAC	1 cut(s)	610
HindII	GTYRAC	1 cut(s)	610
HinfI	GANTC	7 cut(s)	206, 352, 730, 785, 1083, 1154, 1335
HphI	GGTGA	7 cut(s)	517, 613, 830, 839, 851, 1492, 1588
Hpy188I	TCNGA	4 cut(s)	211, 805, 1159, 1323
HpyAV	CCTTC	4 cut(s)	556, 988, 1531, 1688
HpyCH4III	ACNGT	3 cut(s)	518, 827, 1243
HpyCH4IV	ACGT	5 cut(s)	378, 533, 678, 866, 1605
HpyCH4V	TGCA	3 cut(s)	9, 113, 1273
HpyF3I	CTNAG	2 cut(s)	97, 590
HpySE526I	ACGT	5 cut(s)	378, 533, 678, 866, 1605
Hsp92I	GRCGYC	4 cut(s)	79, 756, 861, 1506
Hsp92II	CATG	3 cut(s)	49, 370, 1657
HspAI	GCGC	2 cut(s)	79, 460
KasI	GGCGCC	1 cut(s)	78
Kpn2I	TCCGGA	2 cut(s)	572, 1228
Kzo9I	GATC	8 cut(s)	12, 264, 277, 603, 1116, 1166, 1326, 1769
LguI	GCTCTTC	1 cut(s)	1070
LmnI	GCTCC	2 cut(s)	240, 1666
Lsp1109I	GCAGC	4 cut(s)	97, 106, 125, 1755
MaeI	CTAG	2 cut(s)	482, 1025
MaeII	ACGT	5 cut(s)	378, 533, 678, 866, 1605
MaeIII	GTNAC	5 cut(s)	304, 740, 818, 827, 1446
MalI	GATC	8 cut(s)	14, 266, 279, 605, 1118, 1168, 1328, 1771
MbiI	CCGCTC	2 cut(s)	235, 1180
MboI	GATC	8 cut(s)	12, 264, 277, 603, 1116, 1166, 1326, 1769
MflI	RGATCY	2 cut(s)	12, 1116
MhlI	GDGCHC	2 cut(s)	314, 1275
MluCI	AATT	8 cut(s)	212, 326, 416, 895, 911, 916, 1597, 1733
MluI	ACGCGT	2 cut(s)	624, 1443
Mly113I	GGCGCC	1 cut(s)	79
MlyI	GAGTC	2 cut(s)	724, 794
MmeI	TCCRAC	5 cut(s)	754, 828, 1365, 1446, 1680
MroI	TCCGGA	2 cut(s)	572, 1228
MseI	TTAA	3 cut(s)	165, 215, 914
MspA1I	CMGCKG	4 cut(s)	60, 119, 244, 1219
MspR9I	CCNGG	9 cut(s)	393, 689, 991, 1164, 1256, 1368, 1549, 1571, 1664
Mva1269I	GAATGC	2 cut(s)	958, 1627
MvaI	CCWGG	2 cut(s)	991, 1256
MvnI	CGCG	9 cut(s)	66, 357, 462, 626, 650, 975, 1007, 1070, 1445
NarI	GGCGCC	1 cut(s)	79
NciI	CCSGG	7 cut(s)	393, 689, 1164, 1368, 1549, 1571, 1664
NdeII	GATC	8 cut(s)	12, 264, 277, 603, 1116, 1166, 1326, 1769
NheI	GCTAGC	1 cut(s)	1024
NlaIII	CATG	3 cut(s)	49, 370, 1657
NlaIV	GGNNCC	4 cut(s)	80, 312, 587, 654
NmeAIII	GCCGAG	2 cut(s)	965, 1719
NmuCI	GTSAC	5 cut(s)	304, 740, 818, 827, 1446
NruI	TCGCGA	1 cut(s)	1070
PagI	TCATGA	1 cut(s)	366
PciSI	GCTCTTC	1 cut(s)	1070
PcsI	WCGNNNNNNNCGW	5 cut(s)	828, 831, 1281, 1335, 1530
PctI	GAATGC	2 cut(s)	958, 1627
PfeI	GAWTC	5 cut(s)	206, 352, 1083, 1154, 1335
Pfl23II	CGTACG	2 cut(s)	340, 1602
PflFI	GACNNNGTC	1 cut(s)	335
PfoI	TCCNGGA	1 cut(s)	1366
PinAI	ACCGGT	1 cut(s)	1461
PleI	GAGTC	2 cut(s)	724, 793
PluTI	GGCGCC	1 cut(s)	82
PmaCI	CACGTG	1 cut(s)	534
PmlI	CACGTG	1 cut(s)	534
PpsI	GAGTC	2 cut(s)	724, 793
Ppu21I	YACGTR	1 cut(s)	534
PpuMI	RGGWCCY	1 cut(s)	586
PshAI	GACNNNNGTC	1 cut(s)	293
PsiI	TTATAA	1 cut(s)	104
Psp5II	RGGWCCY	1 cut(s)	586
Psp6I	CCWGG	2 cut(s)	989, 1254
PspCI	CACGTG	1 cut(s)	534
PspFI	CCCAGC	1 cut(s)	1669
PspGI	CCWGG	2 cut(s)	989, 1254
PspLI	CGTACG	2 cut(s)	340, 1602
PspN4I	GGNNCC	4 cut(s)	80, 312, 587, 654
PspOMI	GGGCCC	1 cut(s)	310
PspPPI	RGGWCCY	1 cut(s)	586
PstI	CTGCAG	1 cut(s)	115
PstNI	CAGNNNCTG	2 cut(s)	119, 1157
PsuI	RGATCY	2 cut(s)	12, 1116
PsyI	GACNNNGTC	1 cut(s)	335
RruI	TCGCGA	1 cut(s)	1070
RsaI	GTAC	8 cut(s)	342, 431, 467, 538, 629, 1406, 1442, 1604
RsaNI	GTAC	8 cut(s)	341, 430, 466, 537, 628, 1405, 1441, 1603
Rsr2I	CGGWCCG	2 cut(s)	685, 1633
RsrII	CGGWCCG	2 cut(s)	685, 1633
SalI	GTCGAC	1 cut(s)	608
SapI	GCTCTTC	1 cut(s)	1070
SaqAI	TTAA	3 cut(s)	165, 215, 914
Sau3AI	GATC	8 cut(s)	12, 264, 277, 603, 1116, 1166, 1326, 1769
SchI	GAGTC	2 cut(s)	724, 794
ScrFI	CCNGG	9 cut(s)	393, 689, 991, 1164, 1256, 1368, 1549, 1571, 1664
SduI	GDGCHC	2 cut(s)	314, 1275
SfcI	CTRYAG	2 cut(s)	111, 523
SfoI	GGCGCC	1 cut(s)	80
SgrDI	CGTCGACG	1 cut(s)	608
SinI	GGWCC	9 cut(s)	295, 493, 586, 652, 685, 1459, 1468, 1633, 1660
SmlI	CTYRAG	3 cut(s)	1058, 1121, 1564
SmoI	CTYRAG	3 cut(s)	1058, 1121, 1564
Sse9I	AATT	8 cut(s)	212, 326, 416, 895, 911, 916, 1597, 1733
SspDI	GGCGCC	1 cut(s)	78
SspMI	CTAG	2 cut(s)	482, 1025
StyD4I	CCNGG	9 cut(s)	391, 687, 989, 1162, 1254, 1366, 1547, 1569, 1662
StyI	CCWWGG	1 cut(s)	1266
TaaI	ACNGT	3 cut(s)	518, 827, 1243
TaiI	ACGT	5 cut(s)	381, 536, 681, 869, 1608
TasI	AATT	8 cut(s)	212, 326, 416, 895, 911, 916, 1597, 1733
TfiI	GAWTC	5 cut(s)	206, 352, 1083, 1154, 1335
Tru1I	TTAA	3 cut(s)	165, 215, 914
Tru9I	TTAA	3 cut(s)	165, 215, 914
TscAI	CASTG	4 cut(s)	297, 1455, 1753, 1794
TseFI	GTSAC	5 cut(s)	304, 740, 818, 827, 1446
TseI	GCWGC	4 cut(s)	110, 113, 119, 1743
Tsp45I	GTSAC	5 cut(s)	304, 740, 818, 827, 1446
TspDTI	ATGAA	4 cut(s)	160, 786, 1300, 1358
TspGWI	ACGGA	3 cut(s)	292, 554, 1347
TspRI	CASTG	4 cut(s)	297, 1455, 1753, 1794
Tth111I	GACNNNGTC	1 cut(s)	335
VneI	GTGCAC	1 cut(s)	1271
VpaK11BI	GGWCC	9 cut(s)	295, 493, 586, 652, 685, 1459, 1468, 1633, 1660
XagI	CCTNNNNNAGG	1 cut(s)	988
XapI	RAATTY	3 cut(s)	326, 916, 1733
XmiI	GTMKAC	2 cut(s)	399, 609
XspI	CTAG	2 cut(s)	482, 1025

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.