Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0006661

Physical Location & Seq

Genomic Coordinates Forward (+)

36575058 .. 36575924

Gene Structure 867 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1274000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 867 bp

ATGGTGTCCAAGATTGTGAAGAAGAATGCCAACAGGCGACGCAATCGCGGAAAGTCCTCGGCCAAGAAGGAGGCAGTCGCGGCCTCCTCCGCAATGCTAGCCTCCCTCTTCGATTGCCCCCGCCGCCTCGTCAAGCTCTTCGCGAAACTTGCGAGAATCGGCACGCCGAACCGCCACAGGGGCTTCAAGATCCTCAAGAAAGCCCAAGTCCACGAGGCGGAGACGGAATCTGAACCCGGATCGGGAAACCCGCTCTGTCGCAAGCTCTTGTTCGAGCCACCGCTTACGCCGCTGCTCTCTCCGGAGAAGATGACGGTGTTTCTCGACCTGGATGAGACCTTGGTGCACGCACAGGCCGACCCGCCGCCGGAGAAGTTCGATTTCATAGTGCGCCCGAGGATCGACGGAATCGAGATGAACTTTTATGTTCGTAAGCGTCCCGGAGTGGACGAGTTGTTGGGGAAGTTAGGGGCGAAGTACGAGGTGGTGGTATTCACGGCGGGGTTGAGAGAGTACGCGTCGCTGGTGCTGGACCGGTTGGACCAGAAACGGGTGATTTCACACCGGCTATATCGGGACGCCTGTAAAGAAGTGGACGGGAAGTTCGTGAAGGACTTGTCCGGGTTGGGGAGGGACTTGAGCCGGGTCGTGATTGTGGACGACAATCCCAATTCGTACGTCTTTCAACCGGACAATGCCGTTCCGGTCCGTCCGTTTGTGGACGACATGGAGGACCGGGAGCTGGGGAGGTTGGTGGAGTTCTTCGAAGGGTTGGACTGTGAAGATATGAGGGACGCTGTGAAGAAATTTCTTGGCAGCACTGGCGGCAGAGACGGCGGTGGATCGAAAGACGGCGAAGCAGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0036211 GO:0042578 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

289

Amino Acids

32.22

Weight (kDa)

9.04

Isoelectric Point (pI)

32.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	105 - 259	2e-52	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006661)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29770 AT1G29780 AT5G45700
fragaria_vesca	FvH4_3g07100 FvH4_3g07110 FvH4_3g36710
malus_domestica	MD00G1007300.v1.1 MD00G1007500.v1.1 MD10G1273900.v1.1 MD10G1274000.v1.1
prunus_persica	Prupe.4G068500_v2.0.a1 Prupe.4G068600_v2.0.a1
pyrus_communis	pycom05g27270 pycom10g22870
rosa_chinensis	RchiOBHm_Chr5g0012001 RchiOBHm_Chr5g0012011
rosa_laevigata	RLG00000031878
rosa_multiflora	Rmu_co8217880.1_g000001
rosa_roxburghii	Rroxscaffold_1G00064580
rosa_rugosa	Rorug04G0460300 Rorug04G0460300
rosa_samantha	Rh5BG088100 Rh5BG088200 Rh5CG102000 Rh5DG088700
rosa_wichuraiana	Rw5G008060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	253
AccII	CGCG	4 cut(s)	48, 80, 143, 518
AccIII	TCCGGA	1 cut(s)	301
AclWI	GGATC	4 cut(s)	184, 247, 407, 850
AcoI	YGGCCR	1 cut(s)	60
AcsI	RAATTY	1 cut(s)	806
AcyI	GRCGYC	1 cut(s)	579
AfaI	GTAC	3 cut(s)	479, 515, 677
AfiI	CCNNNNNNNGG	7 cut(s)	178, 217, 242, 367, 550, 627, 742
AflIII	ACRYGT	1 cut(s)	516
AgeI	ACCGGT	1 cut(s)	534
AgsI	TTSAA	2 cut(s)	187, 686
AjnI	CCWGG	1 cut(s)	327
AloI	GAACNNNNNNTCC	2 cut(s)	587, 619
AluBI	AGCT	3 cut(s)	136, 265, 742
AluI	AGCT	3 cut(s)	136, 265, 742
Alw21I	GWGCWC	1 cut(s)	348
Alw26I	GTCTC	3 cut(s)	215, 329, 825
Alw44I	GTGCAC	1 cut(s)	344
AlwI	GGATC	4 cut(s)	184, 247, 407, 850
Ama87I	CYCGRG	1 cut(s)	394
Aor13HI	TCCGGA	1 cut(s)	301
AoxI	GGCC	3 cut(s)	60, 81, 354
ApaLI	GTGCAC	1 cut(s)	344
ApeKI	GCWGC	2 cut(s)	292, 816
ApoI	RAATTY	1 cut(s)	806
ArsI	GACNNNNNNTTYG	2 cut(s)	587, 619
AsiGI	ACCGGT	1 cut(s)	534
AspLEI	GCGC	1 cut(s)	393
AspS9I	GGNCC	4 cut(s)	532, 541, 706, 733
AsuC2I	CCSGG	5 cut(s)	237, 441, 622, 644, 737
AsuHPI	GGTGA	1 cut(s)	565
AsuII	TTCGAA	1 cut(s)	765
AsuNHI	GCTAGC	1 cut(s)	97
AvaI	CYCGRG	1 cut(s)	394
AvaII	GGWCC	4 cut(s)	532, 541, 706, 733
BaeGI	GKGCMC	1 cut(s)	348
BauI	CACGAG	1 cut(s)	212
Bbv12I	GWGCWC	1 cut(s)	348
BbvI	GCAGC	2 cut(s)	279, 828
BceAI	ACGGC	3 cut(s)	513, 683, 850
BciT130I	CCWGG	1 cut(s)	329
BcnI	CCSGG	5 cut(s)	237, 441, 622, 644, 737
BcoDI	GTCTC	3 cut(s)	215, 329, 825
BfaI	CTAG	1 cut(s)	98
BglI	GCCNNNNNGGC	1 cut(s)	180
BisI	GCNGC	7 cut(s)	81, 124, 290, 293, 365, 817, 826
BlsI	GCNGC	7 cut(s)	82, 125, 291, 294, 366, 818, 827
Bme1390I	CCNGG	6 cut(s)	237, 329, 441, 622, 644, 737
Bme18I	GGWCC	4 cut(s)	532, 541, 706, 733
BmeT110I	CYCGRG	1 cut(s)	394
BmgT120I	GGNCC	4 cut(s)	532, 541, 706, 733
BmrFI	CCNGG	6 cut(s)	237, 329, 441, 622, 644, 737
BmtI	GCTAGC	1 cut(s)	101
Bpu14I	TTCGAA	1 cut(s)	765
BpuEI	CTTGAG	2 cut(s)	179, 658
BpuMI	CCSGG	5 cut(s)	237, 441, 622, 644, 737
BsaHI	GRCGYC	1 cut(s)	579
BsaI	GGTCTC	1 cut(s)	329
BsaJI	CCNNGG	3 cut(s)	57, 339, 395
BsaWI	WCCGGW	4 cut(s)	301, 534, 688, 703
Bsc4I	CCNNNNNNNGG	7 cut(s)	178, 217, 242, 367, 550, 627, 742
Bse118I	RCCGGY	2 cut(s)	534, 564
Bse1I	ACTGG	1 cut(s)	826
Bse3DI	GCAATG	1 cut(s)	99
BseAI	TCCGGA	1 cut(s)	301
BseBI	CCWGG	1 cut(s)	329
BseDI	CCNNGG	3 cut(s)	57, 339, 395
BseGI	GGATG	1 cut(s)	337
BseLI	CCNNNNNNNGG	7 cut(s)	178, 217, 242, 367, 550, 627, 742
BseMI	GCAATG	1 cut(s)	99
BseNI	ACTGG	1 cut(s)	826
BseRI	GAGGAG	1 cut(s)	76
BseSI	GKGCMC	1 cut(s)	348
BseXI	GCAGC	2 cut(s)	279, 828
BseYI	CCCAGC	1 cut(s)	742
Bsh1236I	CGCG	4 cut(s)	48, 80, 143, 518
BshFI	GGCC	3 cut(s)	62, 83, 356
BshTI	ACCGGT	1 cut(s)	534
BsiHKAI	GWGCWC	1 cut(s)	348
BsiHKCI	CYCGRG	1 cut(s)	394
BsiWI	CGTACG	1 cut(s)	675
BslFI	GGGAC	4 cut(s)	423, 590, 647, 806
BslI	CCNNNNNNNGG	7 cut(s)	178, 217, 242, 367, 550, 627, 742
BsmAI	GTCTC	3 cut(s)	215, 329, 825
BsmBI	CGTCTC	2 cut(s)	215, 825
BsmFI	GGGAC	4 cut(s)	423, 590, 647, 806
BsmI	GAATGC	1 cut(s)	31
BsnI	GGCC	3 cut(s)	62, 83, 356
Bso31I	GGTCTC	1 cut(s)	329
BsoBI	CYCGRG	1 cut(s)	394
Bsp119I	TTCGAA	1 cut(s)	765
Bsp1286I	GDGCHC	1 cut(s)	348
Bsp13I	TCCGGA	1 cut(s)	301
Bsp143I	GATC	4 cut(s)	189, 239, 399, 842
Bsp68I	TCGCGA	1 cut(s)	143
BspANI	GGCC	3 cut(s)	62, 83, 356
BspEI	TCCGGA	1 cut(s)	301
BspFNI	CGCG	4 cut(s)	48, 80, 143, 518
BspOI	GCTAGC	1 cut(s)	101
BspPI	GGATC	4 cut(s)	184, 247, 407, 850
BspQI	GCTCTTC	1 cut(s)	143
BspT104I	TTCGAA	1 cut(s)	765
BspTNI	GGTCTC	1 cut(s)	329
BsrBI	CCGCTC	1 cut(s)	253
BsrDI	GCAATG	1 cut(s)	99
BsrFI	RCCGGY	2 cut(s)	534, 564
BsrI	ACTGG	1 cut(s)	826
BssAI	RCCGGY	2 cut(s)	534, 564
BssECI	CCNNGG	3 cut(s)	57, 339, 395
BssMI	GATC	4 cut(s)	189, 239, 399, 842
BssNI	GRCGYC	1 cut(s)	579
BssSI	CACGAG	1 cut(s)	212
BssT1I	CCWWGG	1 cut(s)	339
Bst2BI	CACGAG	1 cut(s)	212
Bst2UI	CCWGG	1 cut(s)	329
Bst4CI	ACNGT	2 cut(s)	316, 779
Bst6I	CTCTTC	2 cut(s)	113, 143
BstACI	GRCGYC	1 cut(s)	579
BstBI	TTCGAA	1 cut(s)	765
BstC8I	GCNNGC	4 cut(s)	99, 164, 263, 348
BstF5I	GGATG	1 cut(s)	337
BstFNI	CGCG	4 cut(s)	48, 80, 143, 518
BstHHI	GCGC	1 cut(s)	393
BstKTI	GATC	4 cut(s)	192, 242, 402, 845
BstMAI	GTCTC	3 cut(s)	215, 329, 825
BstMBI	GATC	4 cut(s)	189, 239, 399, 842
BstNI	CCWGG	1 cut(s)	329
BstSCI	CCNGG	6 cut(s)	235, 327, 439, 620, 642, 735
BstSLI	GKGCMC	1 cut(s)	348
BstUI	CGCG	4 cut(s)	48, 80, 143, 518
BstV1I	GCAGC	2 cut(s)	279, 828
BstX2I	RGATCY	1 cut(s)	189
BstYI	RGATCY	1 cut(s)	189
BsuRI	GGCC	3 cut(s)	62, 83, 356
BtsCI	GGATG	1 cut(s)	337
BtsI	GCAGTG	1 cut(s)	867
BtsIMutI	CAGTG	2 cut(s)	819, 867
BtuMI	TCGCGA	1 cut(s)	143
Cac8I	GCNNGC	4 cut(s)	99, 164, 263, 348
CfoI	GCGC	1 cut(s)	393
Cfr10I	RCCGGY	2 cut(s)	534, 564
Cfr13I	GGNCC	4 cut(s)	532, 541, 706, 733
CpoI	CGGWCCG	1 cut(s)	706
CseI	GACGC	5 cut(s)	48, 425, 507, 587, 803
Csp6I	GTAC	3 cut(s)	478, 514, 676
CspAI	ACCGGT	1 cut(s)	534
CspI	CGGWCCG	1 cut(s)	706
CviAII	CATG	1 cut(s)	727
CviQI	GTAC	3 cut(s)	478, 514, 676
DpnI	GATC	4 cut(s)	191, 241, 401, 844
DpnII	GATC	4 cut(s)	189, 239, 399, 842
EaeI	YGGCCR	1 cut(s)	60
Eam1104I	CTCTTC	2 cut(s)	113, 143
EarI	CTCTTC	2 cut(s)	113, 143
EciI	GGCGGA	1 cut(s)	233
Eco130I	CCWWGG	1 cut(s)	339
Eco31I	GGTCTC	1 cut(s)	329
Eco47I	GGWCC	4 cut(s)	532, 541, 706, 733
Eco88I	CYCGRG	1 cut(s)	394
EcoRII	CCWGG	1 cut(s)	327
EcoT14I	CCWWGG	1 cut(s)	339
ErhI	CCWWGG	1 cut(s)	339
Esp3I	CGTCTC	2 cut(s)	215, 825
FaeI	CATG	1 cut(s)	730
FaiI	YATR	5 cut(s)	386, 426, 571, 728, 788
FaqI	GGGAC	4 cut(s)	423, 590, 647, 806
FatI	CATG	1 cut(s)	726
FauI	CCCGC	4 cut(s)	128, 258, 369, 493
Fnu4HI	GCNGC	7 cut(s)	81, 124, 290, 293, 365, 817, 826
FokI	GGATG	1 cut(s)	344
Fsp4HI	GCNGC	7 cut(s)	81, 124, 290, 293, 365, 817, 826
FspBI	CTAG	1 cut(s)	98
GlaI	GCGC	1 cut(s)	392
GluI	GCNGC	7 cut(s)	81, 124, 290, 293, 365, 817, 826
GsaI	CCCAGC	1 cut(s)	746
HaeIII	GGCC	3 cut(s)	62, 83, 356
HgaI	GACGC	5 cut(s)	48, 425, 507, 587, 803
HhaI	GCGC	1 cut(s)	393
Hin1I	GRCGYC	1 cut(s)	579
Hin1II	CATG	1 cut(s)	730
Hin6I	GCGC	1 cut(s)	391
HinP1I	GCGC	1 cut(s)	391
HinfI	GANTC	3 cut(s)	156, 227, 408
HphI	GGTGA	1 cut(s)	565
Hpy166II	GTNNAC	6 cut(s)	211, 346, 448, 595, 658, 721
Hpy188I	TCNGA	1 cut(s)	232
Hpy8I	GTNNAC	6 cut(s)	211, 346, 448, 595, 658, 721
Hpy99I	CGWCG	3 cut(s)	42, 407, 523
HpyAV	CCTTC	3 cut(s)	61, 604, 761
HpyCH4III	ACNGT	2 cut(s)	316, 779
HpyCH4IV	ACGT	1 cut(s)	678
HpyCH4V	TGCA	1 cut(s)	346
HpySE526I	ACGT	1 cut(s)	678
Hsp92I	GRCGYC	1 cut(s)	579
Hsp92II	CATG	1 cut(s)	730
HspAI	GCGC	1 cut(s)	391
Kpn2I	TCCGGA	1 cut(s)	301
Kzo9I	GATC	4 cut(s)	189, 239, 399, 842
LguI	GCTCTTC	1 cut(s)	143
LmnI	GCTCC	1 cut(s)	739
Lsp1109I	GCAGC	2 cut(s)	279, 828
MaeI	CTAG	1 cut(s)	98
MaeII	ACGT	1 cut(s)	678
MalI	GATC	4 cut(s)	191, 241, 401, 844
MbiI	CCGCTC	1 cut(s)	253
MboI	GATC	4 cut(s)	189, 239, 399, 842
MboII	GAAGA	8 cut(s)	31, 34, 100, 130, 319, 754, 794, 814
MflI	RGATCY	1 cut(s)	189
MhlI	GDGCHC	1 cut(s)	348
MluCI	AATT	2 cut(s)	670, 806
MluI	ACGCGT	1 cut(s)	516
MmeI	TCCRAC	2 cut(s)	519, 753
MroI	TCCGGA	1 cut(s)	301
MspA1I	CMGCKG	1 cut(s)	292
MspR9I	CCNGG	6 cut(s)	237, 329, 441, 622, 644, 737
Mva1269I	GAATGC	1 cut(s)	31
MvaI	CCWGG	1 cut(s)	329
MvnI	CGCG	4 cut(s)	48, 80, 143, 518
NciI	CCSGG	5 cut(s)	237, 441, 622, 644, 737
NdeII	GATC	4 cut(s)	189, 239, 399, 842
NheI	GCTAGC	1 cut(s)	97
NlaIII	CATG	1 cut(s)	730
NmeAIII	GCCGAG	1 cut(s)	38
NruI	TCGCGA	1 cut(s)	143
NspV	TTCGAA	1 cut(s)	765
PciSI	GCTCTTC	1 cut(s)	143
PcsI	WCGNNNNNNNCGW	3 cut(s)	354, 408, 603
PctI	GAATGC	1 cut(s)	31
PfeI	GAWTC	3 cut(s)	156, 227, 408
Pfl23II	CGTACG	1 cut(s)	675
PfoI	TCCNGGA	1 cut(s)	439
PinAI	ACCGGT	1 cut(s)	534
PkrI	GCNGC	7 cut(s)	82, 125, 291, 294, 366, 818, 827
Psp6I	CCWGG	1 cut(s)	327
PspFI	CCCAGC	1 cut(s)	742
PspGI	CCWGG	1 cut(s)	327
PspLI	CGTACG	1 cut(s)	675
PspPI	GGNCC	4 cut(s)	532, 541, 706, 733
PsuI	RGATCY	1 cut(s)	189
RruI	TCGCGA	1 cut(s)	143
RsaI	GTAC	3 cut(s)	479, 515, 677
RsaNI	GTAC	3 cut(s)	478, 514, 676
Rsr2I	CGGWCCG	1 cut(s)	706
RsrII	CGGWCCG	1 cut(s)	706
SapI	GCTCTTC	1 cut(s)	143
SatI	GCNGC	7 cut(s)	81, 124, 290, 293, 365, 817, 826
Sau3AI	GATC	4 cut(s)	189, 239, 399, 842
Sau96I	GGNCC	4 cut(s)	532, 541, 706, 733
ScrFI	CCNGG	6 cut(s)	237, 329, 441, 622, 644, 737
SduI	GDGCHC	1 cut(s)	348
SetI	ASST	8 cut(s)	138, 267, 330, 341, 486, 681, 744, 752
SfuI	TTCGAA	1 cut(s)	765
SinI	GGWCC	4 cut(s)	532, 541, 706, 733
SmlI	CTYRAG	2 cut(s)	194, 637
SmoI	CTYRAG	2 cut(s)	194, 637
Sse9I	AATT	2 cut(s)	670, 806
SspMI	CTAG	1 cut(s)	98
StyD4I	CCNGG	6 cut(s)	235, 327, 439, 620, 642, 735
StyI	CCWWGG	1 cut(s)	339
TaaI	ACNGT	2 cut(s)	316, 779
TaiI	ACGT	1 cut(s)	681
TaqI	TCGA	8 cut(s)	111, 273, 324, 378, 402, 411, 765, 845
TasI	AATT	2 cut(s)	670, 806
TauI	GCSGC	5 cut(s)	83, 126, 292, 367, 828
TfiI	GAWTC	3 cut(s)	156, 227, 408
TscAI	CASTG	2 cut(s)	826, 867
TseI	GCWGC	2 cut(s)	292, 816
TspDTI	ATGAA	2 cut(s)	373, 431
TspGWI	ACGGA	4 cut(s)	239, 420, 698, 702
TspRI	CASTG	2 cut(s)	826, 867
VneI	GTGCAC	1 cut(s)	344
VpaK11BI	GGWCC	4 cut(s)	532, 541, 706, 733
XapI	RAATTY	1 cut(s)	806
XspI	CTAG	1 cut(s)	98

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.