AT1G30475

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0007665
Physical Location & Seq
Reverse (-)
10788226 .. 10790241
2016 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30475.1

Sequence Viewer

Length: 576 bp
ATGATCGAGAAGCTTCGAGGAGCAACCAAAATCCCAAATTTTTCCTCGACAAAAAATCTACCTGCTTTGATCCAAATCTCTATGGTTATGCCGGTAATGAATCCATCATTGTGTATTGGTACACAAACATCGGTGTACCTCGCTCCTCGTGAGAAATCTTTTACGAGTAATGGAAACTCTCAAAGGCGTTATCCTACAAGCGTTTCTATGCAACGCCAGCCTTGGAGCCAAAGTAAATTCCCTTCTCTCCAGGATGCAGTGCAACCAGTTGTCGTCTCTAAGAATAAAAGACCAGTCTTTGCATGTAACTCTGCGCTGAATTCAAAATGCAGTCAAGGACAAACACAGACCGTTACACGGCAATCACCAACAATCACACAAGCTCCTACTCATGGAAAAGTTAAATCCCCAAAGCTTGATGACGGTGGAAACGGGTTCCCACCTCGGGATGATGGTGGTGGTGGTGGTGGCGGAGGCGGTGGAGGGAGCTCTTCAGGTGGATTCTTCCTTTTCGGGTTTCTTTTGTTCATGGGATACTTGAAAGATTTGGAGGGCGAACATGAAAACAACCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

191

Amino Acids

20.41

Weight (kDa)

9.75

Isoelectric Point (pI)

54.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 70
AciI CCGC 2 cut(s) 471, 477
AclWI GGATC 1 cut(s) 64
AcsI RAATTY 3 cut(s) 37, 236, 319
AcuI CTGAAG 1 cut(s) 477
AfaI GTAC 2 cut(s) 121, 137
AfiI CCNNNNNNNGG 3 cut(s) 357, 392, 445
AgsI TTSAA 2 cut(s) 324, 541
AjnI CCWGG 1 cut(s) 249
AluBI AGCT 4 cut(s) 13, 383, 415, 489
AluI AGCT 4 cut(s) 13, 383, 415, 489
Alw21I GWGCWC 1 cut(s) 491
Alw26I GTCTC 1 cut(s) 280
AlwI GGATC 1 cut(s) 64
Ama87I CYCGRG 1 cut(s) 444
ApoI RAATTY 3 cut(s) 37, 236, 319
AspLEI GCGC 1 cut(s) 316
AsuHPI GGTGA 1 cut(s) 357
AvaI CYCGRG 1 cut(s) 444
BanII GRGCYC 1 cut(s) 491
BarI GAAGNNNNNNTAC 2 cut(s) 226, 258
BauI CACGAG 1 cut(s) 147
Bbv12I GWGCWC 1 cut(s) 491
BccI CCATC 2 cut(s) 112, 446
BceAI ACGGC 1 cut(s) 374
BciT130I CCWGG 1 cut(s) 251
BciVI GTATCC 1 cut(s) 527
BcoDI GTCTC 1 cut(s) 280
BfuAI ACCTGC 1 cut(s) 70
BfuI GTATCC 1 cut(s) 527
Bme1390I CCNGG 1 cut(s) 251
BmeT110I CYCGRG 1 cut(s) 444
BmiI GGNNCC 2 cut(s) 227, 437
BmrFI CCNGG 1 cut(s) 251
BmsI GCATC 1 cut(s) 244
BpmI CTGGAG 1 cut(s) 233
BsaBI GATNNNNATC 1 cut(s) 74
BsaJI CCNNGG 2 cut(s) 221, 443
BsaXI ACNNNNNCTCC 4 cut(s) 217, 247, 367, 397
Bsc4I CCNNNNNNNGG 3 cut(s) 357, 392, 445
Bse118I RCCGGY 1 cut(s) 91
Bse1I ACTGG 2 cut(s) 266, 293
Bse8I GATNNNNATC 1 cut(s) 74
BseBI CCWGG 1 cut(s) 251
BseDI CCNNGG 2 cut(s) 221, 443
BseGI GGATG 2 cut(s) 259, 454
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 3 cut(s) 357, 392, 445
BseNI ACTGG 2 cut(s) 266, 293
BseRI GAGGAG 2 cut(s) 33, 135
BsiHKAI GWGCWC 1 cut(s) 491
BsiHKCI CYCGRG 1 cut(s) 444
BsiSI CCGG 1 cut(s) 92
BslI CCNNNNNNNGG 3 cut(s) 357, 392, 445
BsmAI GTCTC 1 cut(s) 280
BsmBI CGTCTC 1 cut(s) 280
BsoBI CYCGRG 1 cut(s) 444
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 2 cut(s) 3, 69
BspACI CCGC 2 cut(s) 471, 477
BspLI GGNNCC 2 cut(s) 227, 437
BspMI ACCTGC 1 cut(s) 70
BspPI GGATC 1 cut(s) 64
BspQI GCTCTTC 1 cut(s) 496
BsrFI RCCGGY 1 cut(s) 91
BsrI ACTGG 2 cut(s) 266, 293
BssAI RCCGGY 1 cut(s) 91
BssECI CCNNGG 2 cut(s) 221, 443
BssMI GATC 2 cut(s) 3, 69
BssSI CACGAG 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 221
Bst2BI CACGAG 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 251
Bst4CI ACNGT 2 cut(s) 352, 425
Bst6I CTCTTC 1 cut(s) 496
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 1 cut(s) 279
BstF5I GGATG 2 cut(s) 259, 454
BstHHI GCGC 1 cut(s) 316
BstKTI GATC 2 cut(s) 6, 72
BstMAI GTCTC 1 cut(s) 280
BstMBI GATC 2 cut(s) 3, 69
BstMWI GCNNNNNNNGC 1 cut(s) 217
BstNI CCWGG 1 cut(s) 251
BstNSI RCATGY 1 cut(s) 306
BstSCI CCNGG 1 cut(s) 249
BsuI GTATCC 1 cut(s) 527
BtsCI GGATG 2 cut(s) 259, 454
BtsI GCAGTG 1 cut(s) 264
BtsIMutI CAGTG 1 cut(s) 264
BveI ACCTGC 1 cut(s) 70
Cac8I GCNNGC 1 cut(s) 218
CfoI GCGC 1 cut(s) 316
Cfr10I RCCGGY 1 cut(s) 91
Csp6I GTAC 2 cut(s) 120, 136
CviAII CATG 4 cut(s) 303, 392, 529, 560
CviJI RGCY 6 cut(s) 13, 220, 228, 383, 415, 489
CviKI_1 RGCY 6 cut(s) 13, 220, 228, 383, 415, 489
CviQI GTAC 2 cut(s) 120, 136
DdeI CTNAG 1 cut(s) 279
DpnI GATC 2 cut(s) 5, 71
DpnII GATC 2 cut(s) 3, 69
Eam1104I CTCTTC 1 cut(s) 496
EarI CTCTTC 1 cut(s) 496
EciI GGCGGA 1 cut(s) 486
Ecl136II GAGCTC 1 cut(s) 489
Eco130I CCWWGG 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 491
Eco53kI GAGCTC 1 cut(s) 489
Eco57I CTGAAG 1 cut(s) 477
Eco88I CYCGRG 1 cut(s) 444
EcoICRI GAGCTC 1 cut(s) 489
EcoRI GAATTC 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 249
EcoT14I CCWWGG 1 cut(s) 221
EcoT38I GRGCYC 1 cut(s) 491
ErhI CCWWGG 1 cut(s) 221
Esp3I CGTCTC 1 cut(s) 280
FaeI CATG 4 cut(s) 306, 395, 532, 563
FaiI YATR 7 cut(s) 83, 89, 209, 304, 393, 530, 561
FatI CATG 4 cut(s) 302, 391, 528, 559
FokI GGATG 2 cut(s) 266, 461
FriOI GRGCYC 1 cut(s) 491
GlaI GCGC 1 cut(s) 315
GsuI CTGGAG 1 cut(s) 233
HapII CCGG 1 cut(s) 92
HhaI GCGC 1 cut(s) 316
Hin1II CATG 4 cut(s) 306, 395, 532, 563
Hin6I GCGC 1 cut(s) 314
HinP1I GCGC 1 cut(s) 314
HindIII AAGCTT 2 cut(s) 11, 413
HinfI GANTC 2 cut(s) 100, 501
HpaII CCGG 1 cut(s) 92
HphI GGTGA 1 cut(s) 357
Hpy166II GTNNAC 2 cut(s) 122, 136
Hpy188III TCNNGA 3 cut(s) 7, 149, 446
Hpy8I GTNNAC 2 cut(s) 122, 136
HpyAV CCTTC 1 cut(s) 252
HpyCH4III ACNGT 2 cut(s) 352, 425
HpyCH4V TGCA 5 cut(s) 211, 257, 262, 302, 330
HpyF10VI GCNNNNNNNGC 1 cut(s) 217
HpyF3I CTNAG 1 cut(s) 279
Hsp92II CATG 4 cut(s) 306, 395, 532, 563
HspAI GCGC 1 cut(s) 314
Kzo9I GATC 2 cut(s) 3, 69
LguI GCTCTTC 1 cut(s) 496
LmnI GCTCC 5 cut(s) 20, 148, 225, 388, 486
LpnPI CCDG 8 cut(s) 75, 105, 230, 236, 263, 279, 306, 480
LweI GCATC 1 cut(s) 244
MaeIII GTNAC 2 cut(s) 305, 352
MalI GATC 2 cut(s) 5, 71
MboI GATC 2 cut(s) 3, 69
MboII GAAGA 2 cut(s) 483, 496
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 3 cut(s) 37, 236, 319
MnlI CCTC 8 cut(s) 11, 55, 149, 156, 453, 467, 476, 544
MseI TTAA 2 cut(s) 402, 574
MslI CAYNNNNRTG 1 cut(s) 109
MspI CCGG 1 cut(s) 92
MspR9I CCNGG 1 cut(s) 251
MvaI CCWGG 1 cut(s) 251
MwoI GCNNNNNNNGC 1 cut(s) 217
NdeII GATC 2 cut(s) 3, 69
NlaIII CATG 4 cut(s) 306, 395, 532, 563
NlaIV GGNNCC 2 cut(s) 227, 437
NspI RCATGY 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 496
PfeI GAWTC 2 cut(s) 100, 501
PfoI TCCNGGA 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 249
PspGI CCWGG 1 cut(s) 249
PspN4I GGNNCC 2 cut(s) 227, 437
RsaI GTAC 2 cut(s) 121, 137
RsaNI GTAC 2 cut(s) 120, 136
RseI CAYNNNNRTG 1 cut(s) 109
SacI GAGCTC 1 cut(s) 491
SapI GCTCTTC 1 cut(s) 496
SaqAI TTAA 2 cut(s) 402, 574
Sau3AI GATC 2 cut(s) 3, 69
ScrFI CCNGG 1 cut(s) 251
SduI GDGCHC 1 cut(s) 491
SetI ASST 8 cut(s) 15, 64, 141, 385, 417, 445, 491, 499
SfaNI GCATC 1 cut(s) 244
SmiMI CAYNNNNRTG 1 cut(s) 109
Sse9I AATT 3 cut(s) 37, 236, 319
SsiI CCGC 2 cut(s) 471, 477
SstI GAGCTC 1 cut(s) 491
StyD4I CCNGG 1 cut(s) 249
StyI CCWWGG 1 cut(s) 221
TaaI ACNGT 2 cut(s) 352, 425
TaqI TCGA 3 cut(s) 6, 16, 47
TasI AATT 3 cut(s) 37, 236, 319
TfiI GAWTC 2 cut(s) 100, 501
Tru1I TTAA 2 cut(s) 402, 574
Tru9I TTAA 2 cut(s) 402, 574
TscAI CASTG 1 cut(s) 264
TspDTI ATGAA 3 cut(s) 113, 517, 576
TspRI CASTG 1 cut(s) 264
XapI RAATTY 3 cut(s) 37, 236, 319
XceI RCATGY 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.