AT1G30500

factor y, subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008331
Physical Location & Seq
Reverse (-)
10804450 .. 10806428
1979 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30500.1

Sequence Viewer

Length: 561 bp
ATGACTTCTTCAATCCATGAGCTTTCTGATAACATTGGAAGTCATGAGAAGCAAGAACAGAGAGATTCTCATTTCCAACCACCAATCCCTTCTGCAAGAAATTATGAATCAATTGTTACAAGTTTAGTCTACTCAGACCCGGGGACTACAAATTCCATGGCACCTGGACAATATCCATATCCAGATCCTTACTACAGAAGCATATTTGCACCGCCTCCACAACCGTATACCGGGTTGATGGGAGTGCAGCAACAAGGCGTTCCTTTACCATCTGATGCAGTCGAGGAACCTGTTTTTGTTAACGCAAAGCAATACCACGGTATACTAAGGCGCAGACAATCAAGAGCAAGACTTGAGTCTCAGAATAAAGTCATCAAGTCACGTAAGCCGTATTTGCATGAATCTCGGCATTTGCATGCGATAAGACGACCAAGAGGATGTGGCGGGCGGTTTCTAAATGCCAAGAAGGAGGATGAGCATCACGAAGACAGTAGTCATGAAGAAAAATCCAACCTTAGCGCTGGTAAATCCGCCATGGCTGCTTCTAGTGGTACATCTTGA

Protein Analysis

186

Amino Acids

20.82

Weight (kDa)

8.97

Isoelectric Point (pI)

66.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFB_NFYA PF02045 96 - 151 1.2e-27 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 160
AccI GTMKAC 3 cut(s) 129, 227, 322
AciI CCGC 4 cut(s) 212, 444, 448, 531
AclWI GGATC 1 cut(s) 179
AcsI RAATTY 1 cut(s) 151
AfaI GTAC 1 cut(s) 553
AfeI AGCGCT 1 cut(s) 520
AfiI CCNNNNNNNGG 1 cut(s) 230
AgsI TTSAA 1 cut(s) 12
AjnI CCWGG 1 cut(s) 163
AluBI AGCT 1 cut(s) 22
AluI AGCT 1 cut(s) 22
Alw26I GTCTC 1 cut(s) 363
AlwI GGATC 1 cut(s) 179
Ama87I CYCGRG 1 cut(s) 139
Aor51HI AGCGCT 1 cut(s) 520
ApeKI GCWGC 2 cut(s) 247, 539
ApoI RAATTY 1 cut(s) 151
AspLEI GCGC 2 cut(s) 333, 521
AsuC2I CCSGG 3 cut(s) 140, 141, 232
AvaI CYCGRG 1 cut(s) 139
BanI GGYRCC 1 cut(s) 160
BbsI GAAGAC 1 cut(s) 492
BbvI GCAGC 2 cut(s) 259, 526
BccI CCATC 2 cut(s) 232, 277
BceAI ACGGC 1 cut(s) 373
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 1 cut(s) 165
BcnI CCSGG 3 cut(s) 140, 141, 232
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 1 cut(s) 546
BfmI CTRYAG 1 cut(s) 193
BfoI RGCGCY 1 cut(s) 522
BisI GCNGC 2 cut(s) 248, 540
BlsI GCNGC 2 cut(s) 249, 541
Bme1390I CCNGG 4 cut(s) 140, 141, 165, 232
BmeT110I CYCGRG 1 cut(s) 139
BmiI GGNNCC 2 cut(s) 162, 288
BmrFI CCNGG 4 cut(s) 140, 141, 165, 232
BmsI GCATC 2 cut(s) 265, 487
BoxI GACNNNNGTC 2 cut(s) 355, 492
BpiI GAAGAC 1 cut(s) 492
BplI GAGNNNNNCTC 2 cut(s) 52, 84
Bpu10I CCTNAGC 1 cut(s) 515
BpuEI CTTGAG 1 cut(s) 374
BpuMI CCSGG 3 cut(s) 140, 141, 232
BsaAI YACGTR 1 cut(s) 383
BsaBI GATNNNNATC 1 cut(s) 477
BsaJI CCNNGG 5 cut(s) 139, 140, 156, 316, 534
Bsc4I CCNNNNNNNGG 1 cut(s) 230
Bse8I GATNNNNATC 1 cut(s) 477
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 5 cut(s) 139, 140, 156, 316, 534
BseGI GGATG 2 cut(s) 443, 478
BseJI GATNNNNATC 1 cut(s) 477
BseLI CCNNNNNNNGG 1 cut(s) 230
BseMII CTCAG 2 cut(s) 147, 374
BseXI GCAGC 2 cut(s) 259, 526
BsgI GTGCAG 1 cut(s) 266
BshNI GGYRCC 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 139
BsiSI CCGG 2 cut(s) 140, 231
BslFI GGGAC 1 cut(s) 157
BslI CCNNNNNNNGG 1 cut(s) 230
BsmAI GTCTC 1 cut(s) 363
BsmFI GGGAC 1 cut(s) 157
BsoBI CYCGRG 1 cut(s) 139
Bsp143I GATC 1 cut(s) 184
Bsp19I CCATGG 2 cut(s) 156, 534
BspACI CCGC 4 cut(s) 212, 444, 448, 531
BspCNI CTCAG 2 cut(s) 146, 373
BspHI TCATGA 2 cut(s) 43, 496
BspLI GGNNCC 2 cut(s) 162, 288
BspPI GGATC 1 cut(s) 179
BspT107I GGYRCC 1 cut(s) 160
BssECI CCNNGG 5 cut(s) 139, 140, 156, 316, 534
BssMI GATC 1 cut(s) 184
BssNAI GTATAC 2 cut(s) 228, 323
BssT1I CCWWGG 2 cut(s) 156, 534
Bst1107I GTATAC 2 cut(s) 228, 323
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 3 cut(s) 225, 320, 491
BstBAI YACGTR 1 cut(s) 383
BstC8I GCNNGC 2 cut(s) 417, 446
BstDEI CTNAG 4 cut(s) 133, 326, 360, 515
BstDSI CCRYGG 3 cut(s) 156, 316, 534
BstF5I GGATG 2 cut(s) 443, 478
BstH2I RGCGCY 1 cut(s) 522
BstHHI GCGC 2 cut(s) 333, 521
BstKTI GATC 1 cut(s) 187
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 1 cut(s) 184
BstMWI GCNNNNNNNGC 2 cut(s) 394, 539
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 1 cut(s) 419
BstPAI GACNNNNGTC 2 cut(s) 355, 492
BstSCI CCNGG 4 cut(s) 138, 139, 163, 230
BstSFI CTRYAG 1 cut(s) 193
BstV1I GCAGC 2 cut(s) 259, 526
BstV2I GAAGAC 1 cut(s) 492
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BstZ17I GTATAC 2 cut(s) 228, 323
BtgI CCRYGG 3 cut(s) 156, 316, 534
BtsCI GGATG 2 cut(s) 443, 478
Cac8I GCNNGC 2 cut(s) 417, 446
CciI TCATGA 2 cut(s) 43, 496
CfoI GCGC 2 cut(s) 333, 521
Cfr9I CCCGGG 1 cut(s) 139
Csp6I GTAC 1 cut(s) 552
CviAII CATG 7 cut(s) 17, 44, 157, 398, 416, 497, 535
CviJI RGCY 3 cut(s) 22, 388, 539
CviKI_1 RGCY 3 cut(s) 22, 388, 539
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 4 cut(s) 133, 326, 360, 515
DpnI GATC 1 cut(s) 186
DpnII GATC 1 cut(s) 184
EciI GGCGGA 1 cut(s) 520
Eco130I CCWWGG 2 cut(s) 156, 534
Eco47III AGCGCT 1 cut(s) 520
Eco88I CYCGRG 1 cut(s) 139
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 2 cut(s) 156, 534
ErhI CCWWGG 2 cut(s) 156, 534
FaeI CATG 7 cut(s) 20, 47, 160, 401, 419, 500, 538
FaqI GGGAC 1 cut(s) 157
FatI CATG 7 cut(s) 16, 43, 156, 397, 415, 496, 534
FauI CCCGC 1 cut(s) 437
FblI GTMKAC 3 cut(s) 129, 227, 322
Fnu4HI GCNGC 2 cut(s) 248, 540
FokI GGATG 2 cut(s) 450, 485
Fsp4HI GCNGC 2 cut(s) 248, 540
FspBI CTAG 1 cut(s) 546
GlaI GCGC 2 cut(s) 332, 520
GluI GCNGC 2 cut(s) 248, 540
HaeII RGCGCY 1 cut(s) 522
HapII CCGG 2 cut(s) 140, 231
HhaI GCGC 2 cut(s) 333, 521
Hin1II CATG 7 cut(s) 20, 47, 160, 401, 419, 500, 538
Hin6I GCGC 2 cut(s) 331, 519
HinP1I GCGC 2 cut(s) 331, 519
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 4 cut(s) 65, 107, 356, 401
HpaI GTTAAC 1 cut(s) 301
HpaII CCGG 2 cut(s) 140, 231
Hpy166II GTNNAC 4 cut(s) 130, 228, 301, 323
Hpy188I TCNGA 4 cut(s) 28, 136, 274, 363
Hpy188III TCNNGA 6 cut(s) 44, 182, 342, 482, 497, 558
Hpy8I GTNNAC 4 cut(s) 130, 228, 301, 323
HpyAV CCTTC 2 cut(s) 99, 460
HpyCH4III ACNGT 3 cut(s) 225, 320, 491
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 6 cut(s) 95, 209, 247, 278, 397, 415
HpyF10VI GCNNNNNNNGC 2 cut(s) 394, 539
HpyF3I CTNAG 4 cut(s) 133, 326, 360, 515
HpySE526I ACGT 1 cut(s) 382
Hsp92II CATG 7 cut(s) 20, 47, 160, 401, 419, 500, 538
HspAI GCGC 2 cut(s) 331, 519
KspAI GTTAAC 1 cut(s) 301
Kzo9I GATC 1 cut(s) 184
LpnPI CCDG 7 cut(s) 150, 153, 177, 195, 244, 303, 507
Lsp1109I GCAGC 2 cut(s) 259, 526
LweI GCATC 2 cut(s) 265, 487
MaeI CTAG 1 cut(s) 546
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 2 cut(s) 115, 378
MalI GATC 1 cut(s) 186
MboI GATC 1 cut(s) 184
MboII GAAGA 2 cut(s) 497, 512
MfeI CAATTG 1 cut(s) 111
MflI RGATCY 1 cut(s) 184
MluCI AATT 3 cut(s) 100, 111, 151
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 2 cut(s) 100, 534
MnlI CCTC 4 cut(s) 225, 277, 428, 463
MseI TTAA 1 cut(s) 300
MslI CAYNNNNRTG 1 cut(s) 414
MspI CCGG 2 cut(s) 140, 231
MspR9I CCNGG 4 cut(s) 140, 141, 165, 232
MunI CAATTG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 2 cut(s) 394, 539
NciI CCSGG 3 cut(s) 140, 141, 232
NcoI CCATGG 2 cut(s) 156, 534
NdeII GATC 1 cut(s) 184
NlaIII CATG 7 cut(s) 20, 47, 160, 401, 419, 500, 538
NlaIV GGNNCC 2 cut(s) 162, 288
NmeAIII GCCGAG 1 cut(s) 385
NmuCI GTSAC 1 cut(s) 378
NspI RCATGY 1 cut(s) 419
PaeI GCATGC 1 cut(s) 419
PagI TCATGA 2 cut(s) 43, 496
PfeI GAWTC 3 cut(s) 65, 107, 401
PkrI GCNGC 2 cut(s) 249, 541
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
Ppu21I YACGTR 1 cut(s) 383
PshAI GACNNNNGTC 2 cut(s) 355, 492
Psp6I CCWGG 1 cut(s) 163
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 2 cut(s) 162, 288
PsuI RGATCY 1 cut(s) 184
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
RseI CAYNNNNRTG 1 cut(s) 414
SaqAI TTAA 1 cut(s) 300
SatI GCNGC 2 cut(s) 248, 540
Sau3AI GATC 1 cut(s) 184
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 4 cut(s) 140, 141, 165, 232
SetI ASST 5 cut(s) 24, 166, 292, 385, 516
SfaNI GCATC 2 cut(s) 265, 487
SfcI CTRYAG 1 cut(s) 193
SmaI CCCGGG 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 414
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
SphI GCATGC 1 cut(s) 419
Sse9I AATT 3 cut(s) 100, 111, 151
SsiI CCGC 4 cut(s) 212, 444, 448, 531
SspMI CTAG 1 cut(s) 546
StyD4I CCNGG 4 cut(s) 138, 139, 163, 230
StyI CCWWGG 2 cut(s) 156, 534
TaaI ACNGT 3 cut(s) 225, 320, 491
TaiI ACGT 1 cut(s) 385
TaqI TCGA 1 cut(s) 282
TasI AATT 3 cut(s) 100, 111, 151
TfiI GAWTC 3 cut(s) 65, 107, 401
Tru1I TTAA 1 cut(s) 300
Tru9I TTAA 1 cut(s) 300
TseFI GTSAC 1 cut(s) 378
TseI GCWGC 2 cut(s) 247, 539
Tsp45I GTSAC 1 cut(s) 378
TspDTI ATGAA 3 cut(s) 120, 414, 513
TspMI CCCGGG 1 cut(s) 139
XapI RAATTY 1 cut(s) 151
XceI RCATGY 1 cut(s) 419
XmaI CCCGGG 1 cut(s) 139
XmiI GTMKAC 3 cut(s) 129, 227, 322
XspI CTAG 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.