Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0008331

Physical Location & Seq

Genomic Coordinates Forward (+)

40831929 .. 40836805

Gene Structure 4877 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1273300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 414 bp

ATGAAACACAGCCATATCCTTCACAGCCCTATGGGGCCGCACAACCAACGGTATATCTTCCCCGTTCATCTTCAGTTGATGGGAATCCAGCAAGCAGGTGTTCCACTGCCGTCAGATGCAGTTGAGGAACCTGTGTTTGTAAATGCAAAACAATATCATGGTATCTTGCGACGTAGGCAGTCTCGTGCTAAAGCAGAATCTGAAAATAAAGCTCTTAAGAATCGTAAGCCATACTTGCATGAATCTCGACACCAGCATGCATTGAGAAGAGCTAGAGGATGCGGGGGTCGCTTTCTTAATGCAAAGAAAAATGAGAACGAACAGGATGAAATGACATCGGGTGACAAATCCCAGTCAAATATCAACTTGAACTCTGATAAAAATGAGCTTGCTTCCTCTGATCGAACGTCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0040034 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04612 ko05152 map04612 map05152

Pfam Domains

Protein Families

CBFB_NFYA

Protein Analysis

138

Amino Acids

15.6

Weight (kDa)

9.93

Isoelectric Point (pI)

74.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBFB_NFYA	PF02045	43 - 98	1.8e-27	CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008331)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30500 AT1G30500
fragaria_vesca	FvH4_3g10080 FvH4_3g10080
malus_domestica	MD05G1273300.v1.1 MD10G1253300.v1.1
prunus_persica	Prupe.4G090200_v2.0.a1 Prupe.4G090200_v2.0.a1
pyrus_communis	pycom05g25500 pycom10g20900
rosa_chinensis	RchiOBHm_Chr5g0015971
rosa_laevigata	RLG00000032233
rosa_multiflora	Rmu_sc0000299.1_g000027 Rmu_sc0001878.1_g000008
rosa_roxburghii	Rroxscaffold_1G00060650
rosa_rugosa	Rorug05G0030600 Rorug05G0030700 Rorug05G0030700
rosa_samantha	Rh5AG124000 Rh5BG122900 Rh5CG134300 Rh5DG122300
rosa_wichuraiana	Rw5G010710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	86
Acc36I	ACCTGC	1 cut(s)	86
AciI	CCGC	2 cut(s)	38, 282
AcuI	CTGAAG	1 cut(s)	56
AflII	CTTAAG	1 cut(s)	215
AgsI	TTSAA	1 cut(s)	370
AluBI	AGCT	3 cut(s)	212, 272, 388
AluI	AGCT	3 cut(s)	212, 272, 388
Alw26I	GTCTC	1 cut(s)	186
AlwNI	CAGNNNCTG	1 cut(s)	200
AoxI	GGCC	1 cut(s)	35
AspS9I	GGNCC	1 cut(s)	35
AsuHPI	GGTGA	1 cut(s)	353
BauI	CACGAG	1 cut(s)	183
BccI	CCATC	1 cut(s)	73
BceAI	ACGGC	1 cut(s)	94
BcgI	CGANNNNNNTGC	2 cut(s)	227, 261
BcoDI	GTCTC	1 cut(s)	186
BfaI	CTAG	1 cut(s)	273
BfrI	CTTAAG	1 cut(s)	215
BfuAI	ACCTGC	1 cut(s)	86
BisI	GCNGC	1 cut(s)	38
BlsI	GCNGC	1 cut(s)	39
BmgT120I	GGNCC	1 cut(s)	35
BmiI	GGNNCC	2 cut(s)	36, 129
BmrI	ACTGGG	1 cut(s)	346
BmsI	GCATC	2 cut(s)	106, 269
BmuI	ACTGGG	1 cut(s)	346
BsaBI	GATNNNNATC	1 cut(s)	83
Bse1I	ACTGG	1 cut(s)	352
Bse8I	GATNNNNATC	1 cut(s)	83
BseGI	GGATG	2 cut(s)	284, 331
BseJI	GATNNNNATC	1 cut(s)	83
BseNI	ACTGG	1 cut(s)	352
BshFI	GGCC	1 cut(s)	37
BsmAI	GTCTC	1 cut(s)	186
BsnI	GGCC	1 cut(s)	37
Bsp143I	GATC	1 cut(s)	400
BspACI	CCGC	2 cut(s)	38, 282
BspANI	GGCC	1 cut(s)	37
BspLI	GGNNCC	2 cut(s)	36, 129
BspMI	ACCTGC	1 cut(s)	86
BspQI	GCTCTTC	1 cut(s)	262
BspTI	CTTAAG	1 cut(s)	215
BsrI	ACTGG	1 cut(s)	352
BssMI	GATC	1 cut(s)	400
BssSI	CACGAG	1 cut(s)	183
Bst2BI	CACGAG	1 cut(s)	183
Bst4CI	ACNGT	1 cut(s)	51
Bst6I	CTCTTC	1 cut(s)	262
BstAFI	CTTAAG	1 cut(s)	215
BstC8I	GCNNGC	3 cut(s)	93, 258, 390
BstF5I	GGATG	2 cut(s)	284, 331
BstKTI	GATC	1 cut(s)	403
BstMAI	GTCTC	1 cut(s)	186
BstMBI	GATC	1 cut(s)	400
BstMWI	GCNNNNNNNGC	3 cut(s)	175, 235, 288
BstNSI	RCATGY	1 cut(s)	260
BsuRI	GGCC	1 cut(s)	37
BtsCI	GGATG	2 cut(s)	284, 331
BtsI	GCAGTG	1 cut(s)	104
BtsIMutI	CAGTG	1 cut(s)	104
BveI	ACCTGC	1 cut(s)	86
Cac8I	GCNNGC	3 cut(s)	93, 258, 390
CaiI	CAGNNNCTG	1 cut(s)	200
Cfr13I	GGNCC	1 cut(s)	35
CviAII	CATG	3 cut(s)	158, 239, 257
CviJI	RGCY	7 cut(s)	12, 27, 37, 212, 229, 272, 388
CviKI_1	RGCY	7 cut(s)	12, 27, 37, 212, 229, 272, 388
DpnI	GATC	1 cut(s)	402
DpnII	GATC	1 cut(s)	400
Eam1104I	CTCTTC	1 cut(s)	262
EarI	CTCTTC	1 cut(s)	262
Eco57I	CTGAAG	1 cut(s)	56
EcoT22I	ATGCAT	1 cut(s)	262
FaeI	CATG	3 cut(s)	161, 242, 260
FaiI	YATR	7 cut(s)	15, 32, 54, 159, 232, 240, 258
FalI	AAGNNNNNCTT	2 cut(s)	218, 250
FatI	CATG	3 cut(s)	157, 238, 256
FauI	CCCGC	1 cut(s)	275
Fnu4HI	GCNGC	1 cut(s)	38
FokI	GGATG	2 cut(s)	291, 338
Fsp4HI	GCNGC	1 cut(s)	38
FspBI	CTAG	1 cut(s)	273
GluI	GCNGC	1 cut(s)	38
HaeIII	GGCC	1 cut(s)	37
Hin1II	CATG	3 cut(s)	161, 242, 260
HinfI	GANTC	4 cut(s)	84, 197, 220, 242
HphI	GGTGA	1 cut(s)	353
Hpy188I	TCNGA	4 cut(s)	115, 202, 376, 400
Hpy188III	TCNNGA	1 cut(s)	246
Hpy99I	CGWCG	1 cut(s)	174
HpyAV	CCTTC	1 cut(s)	29
HpyCH4III	ACNGT	1 cut(s)	51
HpyCH4IV	ACGT	2 cut(s)	172, 407
HpyCH4V	TGCA	5 cut(s)	119, 146, 238, 260, 302
HpyF10VI	GCNNNNNNNGC	3 cut(s)	175, 235, 288
HpySE526I	ACGT	2 cut(s)	172, 407
Hsp92II	CATG	3 cut(s)	161, 242, 260
Kzo9I	GATC	1 cut(s)	400
LguI	GCTCTTC	1 cut(s)	262
LpnPI	CCDG	6 cut(s)	81, 101, 144, 266, 308, 365
LweI	GCATC	2 cut(s)	106, 269
MaeI	CTAG	1 cut(s)	273
MaeII	ACGT	2 cut(s)	172, 407
MaeIII	GTNAC	1 cut(s)	341
MalI	GATC	1 cut(s)	402
MboI	GATC	1 cut(s)	400
MboII	GAAGA	3 cut(s)	49, 62, 279
MnlI	CCTC	3 cut(s)	118, 269, 406
Mph1103I	ATGCAT	1 cut(s)	262
MseI	TTAA	2 cut(s)	216, 297
MslI	CAYNNNNRTG	1 cut(s)	255
MspCI	CTTAAG	1 cut(s)	215
MwoI	GCNNNNNNNGC	3 cut(s)	175, 235, 288
NdeII	GATC	1 cut(s)	400
NlaIII	CATG	3 cut(s)	161, 242, 260
NlaIV	GGNNCC	2 cut(s)	36, 129
NmuCI	GTSAC	1 cut(s)	341
NsiI	ATGCAT	1 cut(s)	262
NspI	RCATGY	1 cut(s)	260
PaeI	GCATGC	1 cut(s)	260
PaqCI	CACCTGC	1 cut(s)	86
PciSI	GCTCTTC	1 cut(s)	262
PfeI	GAWTC	4 cut(s)	84, 197, 220, 242
PkrI	GCNGC	1 cut(s)	39
PspN4I	GGNNCC	2 cut(s)	36, 129
PspPI	GGNCC	1 cut(s)	35
PstNI	CAGNNNCTG	1 cut(s)	200
RseI	CAYNNNNRTG	1 cut(s)	255
SapI	GCTCTTC	1 cut(s)	262
SaqAI	TTAA	2 cut(s)	216, 297
SatI	GCNGC	1 cut(s)	38
Sau3AI	GATC	1 cut(s)	400
Sau96I	GGNCC	1 cut(s)	35
SetI	ASST	7 cut(s)	100, 133, 175, 214, 274, 390, 410
SfaNI	GCATC	2 cut(s)	106, 269
SmiMI	CAYNNNNRTG	1 cut(s)	255
SmlI	CTYRAG	1 cut(s)	215
SmoI	CTYRAG	1 cut(s)	215
SphI	GCATGC	1 cut(s)	260
SsiI	CCGC	2 cut(s)	38, 282
SspMI	CTAG	1 cut(s)	273
TaaI	ACNGT	1 cut(s)	51
TaiI	ACGT	2 cut(s)	175, 410
TaqI	TCGA	2 cut(s)	247, 403
TauI	GCSGC	1 cut(s)	40
TfiI	GAWTC	4 cut(s)	84, 197, 220, 242
Tru1I	TTAA	2 cut(s)	216, 297
Tru9I	TTAA	2 cut(s)	216, 297
TscAI	CASTG	1 cut(s)	111
TseFI	GTSAC	1 cut(s)	341
Tsp45I	GTSAC	1 cut(s)	341
TspDTI	ATGAA	4 cut(s)	17, 56, 255, 342
TspRI	CASTG	1 cut(s)	111
Vha464I	CTTAAG	1 cut(s)	215
XceI	RCATGY	1 cut(s)	260
XspI	CTAG	1 cut(s)	273
Zsp2I	ATGCAT	1 cut(s)	262

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.