Rh5CG134300

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0008331
Physical Location & Seq
Reverse (-)
11218395 .. 11226081
7687 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG134300.1

Sequence Viewer

Length: 1260 bp
ATGGCACAGAGTCTTCTGAAACTACCCTGGTCTATGCCTCTATGGCACACCATCGACACGGGTGTGGAGTTATCCGGCTACCACGAAGATTGGAAGGACGTGGAGAAGATGTGCCGCAACGCCATTGATCGCAGCTCTGGTCAATTGGTCGATATCTCGTGTGTATATGGCAGTAACGACCTCCTTAAGTACGTCGGTGACAGATGCAATGGTATCAGACGACTAACTTTGCATTGGTGCTCTGGAATAATAGATGGTGTTTTGAATGAAGTAGCTTATAAATTTCCACTATTGGAGGAGATTCACCTGTTCAAACTTGATAGATGCTCACTCTCGCCTAAAACTCTGGAGATGATTGGGCGATCATGCCCTATTTTGAAATCATTCAAATGGTACAAAATCATATGCAAGAGTACTGTTAGAGGTGGACAGGATGATGACTACGCAAAGGCTATAGCAGGAACCATGCGGGGTCTACAGCACCTCTCACTTTCTCGGTTAAATCTGACTAATAACGCTATGAAGGAAATTCTTGATTATTGTCCTTACCTCGAGTCACTTGATTTGGGCTATGTTTTTCGTGGAAGGTTTTGGATTCGTAAGAGGAGATTTAGAAACAAGAATGAAGGAAAAAAATCAAAATTTGTAGCTGCCTTATCCAGTGATGGCCTAGATAGTAGTGAATCTGATGATCAGCAAAAGCATTCAGAGTCTCCGATGATTTCTTCCTCACCTGCACCTGGAATGTCTAATCCTGGAATTCCAGGCCCTATTCAGTATGTAGCCCCTCCACAGATTGGAGCAGGACATGCAGTGGCGCCAGGAGCCTACCCATATCCGGACCCTTACTACAGAAGCATCTTTGCTCCTTATGACACACAACCATATCCTCCACAACCCTATGGGGGGCAACCAACGGTCCATCTTCAGTTAATGGGAATTCAGCAAGCAGGAGTTCCATTGCCAACAGATGCAGTTGAGGAACCTGTGTTTGTAAATGCAAAACAATATCATGGCATCTTGCGACGTCGGCAGTCTCGTGCTAAAGCAGAATCTGAAAACAAAGCTCTAAAATCTCGGAAGCCTTACTTGCATGAATCTCGACACCTGCATGCACTGAGAAGAGCTAGAGGATGTGGGGGTCGGTTTCTAAATGCAAAGAAAGATGAGAATGAACAGAGTGAAGGAGACAATTCACAGGCAATTATCAACTTGAACTCTGATAAAAGCGAGCGTTCTTCCTCGGATAGAACATCCTAA

Protein Analysis

419

Amino Acids

47.06

Weight (kDa)

9.02

Isoelectric Point (pI)

58.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFB_NFYA PF02045 328 - 383 2.3e-26 CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 279
AarI CACCTGC 2 cut(s) 742, 1116
AatII GACGTC 1 cut(s) 1030
Acc36I ACCTGC 2 cut(s) 742, 1116
AccB1I GGYRCC 1 cut(s) 817
AccB7I CCANNNNNTGG 1 cut(s) 797
AccI GTMKAC 1 cut(s) 475
AccIII TCCGGA 1 cut(s) 838
AciI CCGC 2 cut(s) 115, 469
AcsI RAATTY 5 cut(s) 281, 528, 641, 759, 939
AcuI CTGAAG 1 cut(s) 911
AcyI GRCGYC 2 cut(s) 818, 1027
AfaI GTAC 3 cut(s) 191, 395, 415
AfiI CCNNNNNNNGG 5 cut(s) 740, 797, 838, 905, 906
AflII CTTAAG 1 cut(s) 185
AgsI TTSAA 5 cut(s) 265, 313, 379, 388, 1216
AjiI CACGTC 1 cut(s) 100
AjnI CCWGG 5 cut(s) 26, 739, 754, 763, 820
AleI CACNNNNGTG 1 cut(s) 62
AluBI AGCT 5 cut(s) 135, 275, 650, 1067, 1127
AluI AGCT 5 cut(s) 135, 275, 650, 1067, 1127
Alw21I GWGCWC 1 cut(s) 242
Alw26I GTCTC 3 cut(s) 717, 1041, 1182
AlwNI CAGNNNCTG 1 cut(s) 1055
Ama87I CYCGRG 1 cut(s) 551
Aor13HI TCCGGA 1 cut(s) 838
AoxI GGCC 2 cut(s) 667, 766
ApeKI GCWGC 2 cut(s) 132, 650
ApoI RAATTY 5 cut(s) 281, 528, 641, 759, 939
Asp700I GAANNNNTTC 1 cut(s) 383
AspLEI GCGC 1 cut(s) 820
AspS9I GGNCC 3 cut(s) 767, 841, 919
AsuHPI GGTGA 3 cut(s) 209, 296, 723
AvaI CYCGRG 1 cut(s) 551
AvaII GGWCC 2 cut(s) 841, 919
BanI GGYRCC 1 cut(s) 817
BauI CACGAG 2 cut(s) 157, 1038
BbsI GAAGAC 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 242
BbvI GCAGC 2 cut(s) 144, 637
BccI CCATC 4 cut(s) 59, 248, 659, 930
BcgI CGANNNNNNTGC 2 cut(s) 1082, 1116
BciT130I CCWGG 5 cut(s) 28, 741, 756, 765, 822
BclI TGATCA 1 cut(s) 691
BcoDI GTCTC 3 cut(s) 717, 1041, 1182
BfaI CTAG 2 cut(s) 671, 1128
BfmI CTRYAG 3 cut(s) 453, 476, 850
BfoI RGCGCY 1 cut(s) 821
BfrI CTTAAG 1 cut(s) 185
BfuAI ACCTGC 2 cut(s) 742, 1116
BglI GCCNNNNNGGC 1 cut(s) 43
BisI GCNGC 3 cut(s) 115, 133, 651
BlsI GCNGC 3 cut(s) 116, 134, 652
BmcAI AGTACT 1 cut(s) 415
Bme1390I CCNGG 5 cut(s) 28, 741, 756, 765, 822
Bme18I GGWCC 2 cut(s) 841, 919
BmeT110I CYCGRG 1 cut(s) 551
BmgBI CACGTC 1 cut(s) 100
BmgT120I GGNCC 3 cut(s) 767, 841, 919
BmiI GGNNCC 5 cut(s) 463, 819, 826, 843, 984
BmrFI CCNGG 5 cut(s) 28, 741, 756, 765, 822
BmsI GCATC 5 cut(s) 194, 314, 867, 961, 1026
BpiI GAAGAC 1 cut(s) 5
BpmI CTGGAG 1 cut(s) 368
BsaHI GRCGYC 2 cut(s) 818, 1027
BsaJI CCNNGG 2 cut(s) 26, 1242
BsaWI WCCGGW 1 cut(s) 838
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 5 cut(s) 740, 797, 838, 905, 906
Bse1I ACTGG 1 cut(s) 660
Bse3DI GCAATG 2 cut(s) 214, 959
BseAI TCCGGA 1 cut(s) 838
BseBI CCWGG 5 cut(s) 28, 741, 756, 765, 822
BseDI CCNNGG 2 cut(s) 26, 1242
BseGI GGATG 3 cut(s) 439, 1139, 1253
BseLI CCNNNNNNNGG 5 cut(s) 740, 797, 838, 905, 906
BseMI GCAATG 2 cut(s) 214, 959
BseMII CTCAG 1 cut(s) 1109
BseNI ACTGG 1 cut(s) 660
BseRI GAGGAG 2 cut(s) 311, 619
BseXI GCAGC 2 cut(s) 144, 637
BsgI GTGCAG 1 cut(s) 720
BshFI GGCC 2 cut(s) 669, 768
BshNI GGYRCC 1 cut(s) 817
BsiHKAI GWGCWC 1 cut(s) 242
BsiHKCI CYCGRG 1 cut(s) 551
BsiSI CCGG 2 cut(s) 75, 839
BslI CCNNNNNNNGG 5 cut(s) 740, 797, 838, 905, 906
BsmAI GTCTC 3 cut(s) 717, 1041, 1182
BsmI GAATGC 1 cut(s) 703
BsnI GGCC 2 cut(s) 669, 768
BsoBI CYCGRG 1 cut(s) 551
Bsp1286I GDGCHC 1 cut(s) 242
Bsp13I TCCGGA 1 cut(s) 838
Bsp143I GATC 3 cut(s) 127, 362, 691
BspACI CCGC 2 cut(s) 115, 469
BspANI GGCC 2 cut(s) 669, 768
BspCNI CTCAG 1 cut(s) 1110
BspEI TCCGGA 1 cut(s) 838
BspLI GGNNCC 5 cut(s) 463, 819, 826, 843, 984
BspMI ACCTGC 2 cut(s) 742, 1116
BspQI GCTCTTC 1 cut(s) 1117
BspT107I GGYRCC 1 cut(s) 817
BspTI CTTAAG 1 cut(s) 185
BsrDI GCAATG 2 cut(s) 214, 959
BsrI ACTGG 1 cut(s) 660
BssECI CCNNGG 2 cut(s) 26, 1242
BssMI GATC 3 cut(s) 127, 362, 691
BssNI GRCGYC 2 cut(s) 818, 1027
BssSI CACGAG 2 cut(s) 157, 1038
Bst2BI CACGAG 2 cut(s) 157, 1038
Bst2UI CCWGG 5 cut(s) 28, 741, 756, 765, 822
Bst4CI ACNGT 2 cut(s) 418, 919
Bst6I CTCTTC 1 cut(s) 1117
BstACI GRCGYC 2 cut(s) 818, 1027
BstAFI CTTAAG 1 cut(s) 185
BstAPI GCANNNNNTGC 1 cut(s) 809
BstC8I GCNNGC 3 cut(s) 948, 1113, 1232
BstDEI CTNAG 1 cut(s) 1118
BstF5I GGATG 3 cut(s) 439, 1139, 1253
BstH2I RGCGCY 1 cut(s) 821
BstHHI GCGC 1 cut(s) 820
BstKTI GATC 3 cut(s) 130, 365, 694
BstMAI GTCTC 3 cut(s) 717, 1041, 1182
BstMBI GATC 3 cut(s) 127, 362, 691
BstMWI GCNNNNNNNGC 5 cut(s) 43, 809, 824, 1030, 1090
BstNI CCWGG 5 cut(s) 28, 741, 756, 765, 822
BstNSI RCATGY 2 cut(s) 812, 1115
BstSCI CCNGG 5 cut(s) 26, 739, 754, 763, 820
BstSFI CTRYAG 3 cut(s) 453, 476, 850
BstV1I GCAGC 2 cut(s) 144, 637
BstV2I GAAGAC 1 cut(s) 5
BsuRI GGCC 2 cut(s) 669, 768
BtrI CACGTC 1 cut(s) 100
BtsCI GGATG 3 cut(s) 439, 1139, 1253
BtsI GCAGTG 1 cut(s) 819
BtsIMutI CAGTG 3 cut(s) 667, 819, 1115
BveI ACCTGC 2 cut(s) 742, 1116
Cac8I GCNNGC 3 cut(s) 948, 1113, 1232
CaiI CAGNNNCTG 1 cut(s) 1055
CfoI GCGC 1 cut(s) 820
Cfr13I GGNCC 3 cut(s) 767, 841, 919
Csp6I GTAC 3 cut(s) 190, 394, 414
CspCI CAANNNNNGTGG 2 cut(s) 71, 106
CviAII CATG 6 cut(s) 366, 466, 809, 1013, 1094, 1112
CviQI GTAC 3 cut(s) 190, 394, 414
DdeI CTNAG 1 cut(s) 1118
DinI GGCGCC 1 cut(s) 819
DpnI GATC 3 cut(s) 129, 364, 693
DpnII GATC 3 cut(s) 127, 362, 691
Eam1104I CTCTTC 1 cut(s) 1117
EarI CTCTTC 1 cut(s) 1117
Eco32I GATATC 1 cut(s) 154
Eco47I GGWCC 2 cut(s) 841, 919
Eco57I CTGAAG 1 cut(s) 911
Eco88I CYCGRG 1 cut(s) 551
EcoO109I RGGNCCY 1 cut(s) 767
EcoRI GAATTC 2 cut(s) 759, 939
EcoRII CCWGG 5 cut(s) 26, 739, 754, 763, 820
EcoRV GATATC 1 cut(s) 154
EgeI GGCGCC 1 cut(s) 819
EheI GGCGCC 1 cut(s) 819
FaeI CATG 6 cut(s) 369, 469, 812, 1016, 1097, 1115
FalI AAGNNNNNCTT 2 cut(s) 1073, 1105
FatI CATG 6 cut(s) 365, 465, 808, 1012, 1093, 1111
FauI CCCGC 1 cut(s) 462
FauNDI CATATG 1 cut(s) 404
FbaI TGATCA 1 cut(s) 691
FblI GTMKAC 1 cut(s) 475
Fnu4HI GCNGC 3 cut(s) 115, 133, 651
FokI GGATG 3 cut(s) 446, 1146, 1240
Fsp4HI GCNGC 3 cut(s) 115, 133, 651
FspBI CTAG 2 cut(s) 671, 1128
GlaI GCGC 1 cut(s) 819
GluI GCNGC 3 cut(s) 115, 133, 651
GsuI CTGGAG 1 cut(s) 368
HaeII RGCGCY 1 cut(s) 821
HaeIII GGCC 2 cut(s) 669, 768
HapII CCGG 2 cut(s) 75, 839
HhaI GCGC 1 cut(s) 820
Hin1I GRCGYC 2 cut(s) 818, 1027
Hin1II CATG 6 cut(s) 369, 469, 812, 1016, 1097, 1115
Hin6I GCGC 1 cut(s) 818
HinP1I GCGC 1 cut(s) 818
HinfI GANTC 8 cut(s) 10, 301, 554, 595, 683, 710, 1052, 1097
HpaII CCGG 2 cut(s) 75, 839
HphI GGTGA 3 cut(s) 209, 296, 723
Hpy166II GTNNAC 2 cut(s) 428, 476
Hpy188III TCNNGA 5 cut(s) 243, 347, 533, 839, 1101
Hpy8I GTNNAC 2 cut(s) 428, 476
Hpy99I CGWCG 3 cut(s) 197, 1029, 1032
HpyAV CCTTC 5 cut(s) 88, 517, 579, 620, 1178
HpyCH4III ACNGT 2 cut(s) 418, 919
HpyCH4IV ACGT 3 cut(s) 99, 192, 1027
HpyF10VI GCNNNNNNNGC 5 cut(s) 43, 809, 824, 1030, 1090
HpyF3I CTNAG 1 cut(s) 1118
HpySE526I ACGT 3 cut(s) 99, 192, 1027
Hsp92I GRCGYC 2 cut(s) 818, 1027
Hsp92II CATG 6 cut(s) 369, 469, 812, 1016, 1097, 1115
HspAI GCGC 1 cut(s) 818
KasI GGCGCC 1 cut(s) 817
Kpn2I TCCGGA 1 cut(s) 838
Ksp22I TGATCA 1 cut(s) 691
Kzo9I GATC 3 cut(s) 127, 362, 691
LguI GCTCTTC 1 cut(s) 1117
LmnI GCTCC 3 cut(s) 800, 824, 871
Lsp1109I GCAGC 2 cut(s) 144, 637
LweI GCATC 5 cut(s) 194, 314, 867, 961, 1026
MaeI CTAG 2 cut(s) 671, 1128
MaeII ACGT 3 cut(s) 99, 192, 1027
MaeIII GTNAC 3 cut(s) 173, 197, 555
MalI GATC 3 cut(s) 129, 364, 693
MboI GATC 3 cut(s) 127, 362, 691
MboII GAAGA 7 cut(s) 5, 98, 118, 717, 917, 1134, 1230
MfeI CAATTG 1 cut(s) 143
MhlI GDGCHC 1 cut(s) 242
MluCI AATT 8 cut(s) 143, 281, 528, 641, 759, 939, 1192, 1203
Mly113I GGCGCC 1 cut(s) 818
MlyI GAGTC 3 cut(s) 19, 563, 719
MroI TCCGGA 1 cut(s) 838
MroXI GAANNNNTTC 1 cut(s) 383
MseI TTAA 3 cut(s) 186, 500, 932
MslI CAYNNNNRTG 3 cut(s) 62, 388, 1110
MspCI CTTAAG 1 cut(s) 185
MspI CCGG 2 cut(s) 75, 839
MspR9I CCNGG 5 cut(s) 28, 741, 756, 765, 822
MunI CAATTG 1 cut(s) 143
Mva1269I GAATGC 1 cut(s) 703
MvaI CCWGG 5 cut(s) 28, 741, 756, 765, 822
MwoI GCNNNNNNNGC 5 cut(s) 43, 809, 824, 1030, 1090
NarI GGCGCC 1 cut(s) 818
NdeI CATATG 1 cut(s) 404
NdeII GATC 3 cut(s) 127, 362, 691
NlaIII CATG 6 cut(s) 369, 469, 812, 1016, 1097, 1115
NlaIV GGNNCC 5 cut(s) 463, 819, 826, 843, 984
NmuCI GTSAC 2 cut(s) 197, 555
NspI RCATGY 2 cut(s) 812, 1115
OliI CACNNNNGTG 1 cut(s) 62
PaeI GCATGC 1 cut(s) 1115
PaeR7I CTCGAG 1 cut(s) 551
PaqCI CACCTGC 2 cut(s) 742, 1116
PciSI GCTCTTC 1 cut(s) 1117
PcsI WCGNNNNNNNCGW 1 cut(s) 1036
PctI GAATGC 1 cut(s) 703
PdmI GAANNNNTTC 1 cut(s) 383
PfeI GAWTC 5 cut(s) 301, 595, 683, 1052, 1097
PflMI CCANNNNNTGG 1 cut(s) 797
PfoI TCCNGGA 1 cut(s) 754
PkrI GCNGC 3 cut(s) 116, 134, 652
PleI GAGTC 3 cut(s) 18, 562, 718
PluTI GGCGCC 1 cut(s) 821
PpsI GAGTC 3 cut(s) 18, 562, 718
PsiI TTATAA 1 cut(s) 279
Psp6I CCWGG 5 cut(s) 26, 739, 754, 763, 820
PspGI CCWGG 5 cut(s) 26, 739, 754, 763, 820
PspN4I GGNNCC 5 cut(s) 463, 819, 826, 843, 984
PspPI GGNCC 3 cut(s) 767, 841, 919
PspXI VCTCGAGB 1 cut(s) 551
PstNI CAGNNNCTG 1 cut(s) 1055
RsaI GTAC 3 cut(s) 191, 395, 415
RsaNI GTAC 3 cut(s) 190, 394, 414
RseI CAYNNNNRTG 3 cut(s) 62, 388, 1110
SapI GCTCTTC 1 cut(s) 1117
SaqAI TTAA 3 cut(s) 186, 500, 932
SatI GCNGC 3 cut(s) 115, 133, 651
Sau3AI GATC 3 cut(s) 127, 362, 691
Sau96I GGNCC 3 cut(s) 767, 841, 919
ScaI AGTACT 1 cut(s) 415
SchI GAGTC 3 cut(s) 19, 563, 719
ScrFI CCNGG 5 cut(s) 28, 741, 756, 765, 822
SduI GDGCHC 1 cut(s) 242
SfaNI GCATC 5 cut(s) 194, 314, 867, 961, 1026
SfcI CTRYAG 3 cut(s) 453, 476, 850
SfoI GGCGCC 1 cut(s) 819
Sfr274I CTCGAG 1 cut(s) 551
SinI GGWCC 2 cut(s) 841, 919
SlaI CTCGAG 1 cut(s) 551
SmiMI CAYNNNNRTG 3 cut(s) 62, 388, 1110
SmlI CTYRAG 2 cut(s) 185, 551
SmoI CTYRAG 2 cut(s) 185, 551
SphI GCATGC 1 cut(s) 1115
Sse9I AATT 8 cut(s) 143, 281, 528, 641, 759, 939, 1192, 1203
SsiI CCGC 2 cut(s) 115, 469
SspDI GGCGCC 1 cut(s) 817
SspMI CTAG 2 cut(s) 671, 1128
StyD4I CCNGG 5 cut(s) 26, 739, 754, 763, 820
TaaI ACNGT 2 cut(s) 418, 919
TaiI ACGT 3 cut(s) 102, 195, 1030
TaqI TCGA 4 cut(s) 54, 150, 552, 1102
TasI AATT 8 cut(s) 143, 281, 528, 641, 759, 939, 1192, 1203
TatI WGTACW 1 cut(s) 413
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 5 cut(s) 301, 595, 683, 1052, 1097
Tru1I TTAA 3 cut(s) 186, 500, 932
Tru9I TTAA 3 cut(s) 186, 500, 932
TscAI CASTG 3 cut(s) 667, 819, 1122
TseFI GTSAC 2 cut(s) 197, 555
TseI GCWGC 2 cut(s) 132, 650
Tsp45I GTSAC 2 cut(s) 197, 555
TspDTI ATGAA 5 cut(s) 282, 536, 639, 1110, 1188
TspRI CASTG 3 cut(s) 667, 819, 1122
Van91I CCANNNNNTGG 1 cut(s) 797
Vha464I CTTAAG 1 cut(s) 185
VpaK11BI GGWCC 2 cut(s) 841, 919
XapI RAATTY 5 cut(s) 281, 528, 641, 759, 939
XceI RCATGY 2 cut(s) 812, 1115
XhoI CTCGAG 1 cut(s) 551
XmiI GTMKAC 1 cut(s) 475
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 2 cut(s) 671, 1128
ZraI GACGTC 1 cut(s) 1028
ZrmI AGTACT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.