Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0008331

Physical Location & Seq

Genomic Coordinates Forward (+)

34584154 .. 34588714

Gene Structure 4561 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1253300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 627 bp

ATGATTTCTTCCGTGCGCGATCATTCTGATAATAGTGATTCTGATGACCAGCAAAGTCATTCAGAGTCTCAAGTTCATTCATCCTTACCTGCAATTGGAATGTCACATCCTGGAGTTCCAACTCCAAATATGCAGTATGCAGTACCTCCACAGGTTGGAACAGGACATGCAGTGGCACCAGGAGGTTACCCCTATCCAGACCCTTACTACAGAAGCATCTTTGCTCCCTATGAAACACAGCCGTATCCTCCACAGCCCTATGGGGCGCAACCAACGGTTCATCTTCAGTTAATGGGAATCCAGCAAGCAGGTGTTCCACTGCCATCAGATGCAGTTGAGGAACCTGTGTTTGTAAATGCAAAACAGTATCATGGTATCTTGCGACGTCGGCAGTCTCGTGCTAAAGCAGAATCTGAAAATAAAGCGCTTAAGAATCGAAAGCCATATTTGCATGAATCTCGACACCAGCATGCATTGAGAAGAGCTAGAGGATGCGGGGGTCGCTTTCTTAATGCGAAGAAAAATGGGAACCAACCGGATGAAATGACATCGGGTGACAAATCCCAGTCAAATATCAATTTGAACACTGATAAAAATGAGCTTGCTTCCTCAGATGGAACATCCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009890 GO:0009892 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0040034 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048506 GO:0048509 GO:0048510 GO:0048519 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04612 ko05152 map04612 map05152

Pfam Domains

Protein Families

CBFB_NFYA

Protein Analysis

209

Amino Acids

22.82

Weight (kDa)

7.91

Isoelectric Point (pI)

65.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CBFB_NFYA	PF02045	114 - 169	4.3e-27	CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0008331)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30500 AT1G30500
fragaria_vesca	FvH4_3g10080 FvH4_3g10080
malus_domestica	MD05G1273300.v1.1 MD10G1253300.v1.1
prunus_persica	Prupe.4G090200_v2.0.a1 Prupe.4G090200_v2.0.a1
pyrus_communis	pycom05g25500 pycom10g20900
rosa_chinensis	RchiOBHm_Chr5g0015971
rosa_laevigata	RLG00000032233
rosa_multiflora	Rmu_sc0000299.1_g000027 Rmu_sc0001878.1_g000008
rosa_roxburghii	Rroxscaffold_1G00060650
rosa_rugosa	Rorug05G0030600 Rorug05G0030700 Rorug05G0030700
rosa_samantha	Rh5AG124000 Rh5BG122900 Rh5CG134300 Rh5DG122300
rosa_wichuraiana	Rw5G010710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	299
AatII	GACGTC	1 cut(s)	388
Acc36I	ACCTGC	2 cut(s)	97, 299
AccB1I	GGYRCC	1 cut(s)	175
AccB7I	CCANNNNNTGG	1 cut(s)	155
AccII	CGCG	1 cut(s)	18
AciI	CCGC	1 cut(s)	495
AcuI	CTGAAG	1 cut(s)	269
AcyI	GRCGYC	1 cut(s)	385
AfaI	GTAC	1 cut(s)	144
AfeI	AGCGCT	1 cut(s)	426
AfiI	CCNNNNNNNGG	2 cut(s)	95, 155
AflII	CTTAAG	1 cut(s)	428
AgsI	TTSAA	1 cut(s)	583
AjnI	CCWGG	2 cut(s)	109, 178
AluBI	AGCT	2 cut(s)	485, 601
AluI	AGCT	2 cut(s)	485, 601
Alw26I	GTCTC	2 cut(s)	72, 399
AlwNI	CAGNNNCTG	1 cut(s)	413
Aor51HI	AGCGCT	1 cut(s)	426
AspLEI	GCGC	3 cut(s)	18, 268, 427
AsuHPI	GGTGA	1 cut(s)	566
BaeI	ACNNNNGTAYC	2 cut(s)	227, 260
BanI	GGYRCC	1 cut(s)	175
BauI	CACGAG	1 cut(s)	396
BccI	CCATC	2 cut(s)	331, 608
BceAI	ACGGC	1 cut(s)	226
BcgI	CGANNNNNNTGC	2 cut(s)	440, 474
BciT130I	CCWGG	2 cut(s)	111, 180
BciVI	GTATCC	1 cut(s)	255
BcoDI	GTCTC	2 cut(s)	72, 399
BfaI	CTAG	1 cut(s)	486
BfmI	CTRYAG	1 cut(s)	208
BfoI	RGCGCY	1 cut(s)	428
BfrI	CTTAAG	1 cut(s)	428
BfuAI	ACCTGC	2 cut(s)	97, 299
BfuI	GTATCC	1 cut(s)	255
Bme1390I	CCNGG	2 cut(s)	111, 180
BmiI	GGNNCC	3 cut(s)	177, 342, 530
BmrFI	CCNGG	2 cut(s)	111, 180
BmrI	ACTGGG	1 cut(s)	559
BmsI	GCATC	3 cut(s)	225, 319, 482
BmuI	ACTGGG	1 cut(s)	559
BpmI	CTGGAG	1 cut(s)	132
BpuEI	CTTGAG	1 cut(s)	54
BsaHI	GRCGYC	1 cut(s)	385
BsaWI	WCCGGW	1 cut(s)	535
Bsc4I	CCNNNNNNNGG	2 cut(s)	95, 155
Bse1I	ACTGG	1 cut(s)	565
BseBI	CCWGG	2 cut(s)	111, 180
BseGI	GGATG	5 cut(s)	80, 106, 497, 544, 620
BseLI	CCNNNNNNNGG	2 cut(s)	95, 155
BseMII	CTCAG	1 cut(s)	624
BseNI	ACTGG	1 cut(s)	565
Bsh1236I	CGCG	1 cut(s)	18
BshNI	GGYRCC	1 cut(s)	175
BsiSI	CCGG	1 cut(s)	536
BslI	CCNNNNNNNGG	2 cut(s)	95, 155
BsmAI	GTCTC	2 cut(s)	72, 399
Bsp143I	GATC	1 cut(s)	19
BspACI	CCGC	1 cut(s)	495
BspCNI	CTCAG	1 cut(s)	623
BspFNI	CGCG	1 cut(s)	18
BspLI	GGNNCC	3 cut(s)	177, 342, 530
BspMI	ACCTGC	2 cut(s)	97, 299
BspQI	GCTCTTC	1 cut(s)	475
BspT107I	GGYRCC	1 cut(s)	175
BspTI	CTTAAG	1 cut(s)	428
BsrI	ACTGG	1 cut(s)	565
BssMI	GATC	1 cut(s)	19
BssNI	GRCGYC	1 cut(s)	385
BssSI	CACGAG	1 cut(s)	396
Bst2BI	CACGAG	1 cut(s)	396
Bst2UI	CCWGG	2 cut(s)	111, 180
Bst4CI	ACNGT	2 cut(s)	277, 366
Bst6I	CTCTTC	1 cut(s)	475
BstACI	GRCGYC	1 cut(s)	385
BstAFI	CTTAAG	1 cut(s)	428
BstC8I	GCNNGC	3 cut(s)	306, 471, 603
BstDEI	CTNAG	1 cut(s)	610
BstEII	GGTNACC	1 cut(s)	185
BstF5I	GGATG	5 cut(s)	80, 106, 497, 544, 620
BstFNI	CGCG	1 cut(s)	18
BstH2I	RGCGCY	1 cut(s)	428
BstHHI	GCGC	3 cut(s)	18, 268, 427
BstKTI	GATC	1 cut(s)	22
BstMAI	GTCTC	2 cut(s)	72, 399
BstMBI	GATC	1 cut(s)	19
BstMWI	GCNNNNNNNGC	3 cut(s)	388, 448, 501
BstNI	CCWGG	2 cut(s)	111, 180
BstNSI	RCATGY	2 cut(s)	170, 473
BstPI	GGTNACC	1 cut(s)	185
BstSCI	CCNGG	2 cut(s)	109, 178
BstSFI	CTRYAG	1 cut(s)	208
BstUI	CGCG	1 cut(s)	18
BsuI	GTATCC	1 cut(s)	255
BtsCI	GGATG	5 cut(s)	80, 106, 497, 544, 620
BtsI	GCAGTG	2 cut(s)	177, 317
BtsIMutI	CAGTG	3 cut(s)	177, 317, 585
BveI	ACCTGC	2 cut(s)	97, 299
Cac8I	GCNNGC	3 cut(s)	306, 471, 603
CaiI	CAGNNNCTG	1 cut(s)	413
CfoI	GCGC	3 cut(s)	18, 268, 427
Csp6I	GTAC	1 cut(s)	143
CviAII	CATG	4 cut(s)	167, 371, 452, 470
CviJI	RGCY	5 cut(s)	241, 256, 442, 485, 601
CviKI_1	RGCY	5 cut(s)	241, 256, 442, 485, 601
CviQI	GTAC	1 cut(s)	143
DdeI	CTNAG	1 cut(s)	610
DpnI	GATC	1 cut(s)	21
DpnII	GATC	1 cut(s)	19
Eam1104I	CTCTTC	1 cut(s)	475
EarI	CTCTTC	1 cut(s)	475
Eco47III	AGCGCT	1 cut(s)	426
Eco57I	CTGAAG	1 cut(s)	269
Eco91I	GGTNACC	1 cut(s)	185
EcoO65I	GGTNACC	1 cut(s)	185
EcoRII	CCWGG	2 cut(s)	109, 178
EcoT22I	ATGCAT	1 cut(s)	475
FaeI	CATG	4 cut(s)	170, 374, 455, 473
FaiI	YATR	9 cut(s)	131, 138, 168, 231, 261, 372, 445, 453, 471
FatI	CATG	4 cut(s)	166, 370, 451, 469
FauI	CCCGC	1 cut(s)	488
FokI	GGATG	5 cut(s)	67, 93, 504, 551, 607
FspBI	CTAG	1 cut(s)	486
GlaI	GCGC	3 cut(s)	17, 267, 426
GsuI	CTGGAG	1 cut(s)	132
HaeII	RGCGCY	1 cut(s)	428
HapII	CCGG	1 cut(s)	536
HhaI	GCGC	3 cut(s)	18, 268, 427
Hin1I	GRCGYC	1 cut(s)	385
Hin1II	CATG	4 cut(s)	170, 374, 455, 473
Hin6I	GCGC	3 cut(s)	16, 266, 425
HinP1I	GCGC	3 cut(s)	16, 266, 425
HinfI	GANTC	6 cut(s)	38, 65, 297, 410, 433, 455
HpaII	CCGG	1 cut(s)	536
HphI	GGTGA	1 cut(s)	566
Hpy188I	TCNGA	6 cut(s)	28, 43, 64, 328, 415, 613
Hpy188III	TCNNGA	2 cut(s)	197, 459
Hpy99I	CGWCG	2 cut(s)	387, 390
HpyCH4III	ACNGT	2 cut(s)	277, 366
HpyCH4IV	ACGT	1 cut(s)	385
HpyCH4V	TGCA	8 cut(s)	92, 133, 140, 170, 332, 359, 451, 473
HpyF10VI	GCNNNNNNNGC	3 cut(s)	388, 448, 501
HpyF3I	CTNAG	1 cut(s)	610
HpySE526I	ACGT	1 cut(s)	385
Hsp92I	GRCGYC	1 cut(s)	385
Hsp92II	CATG	4 cut(s)	170, 374, 455, 473
HspAI	GCGC	3 cut(s)	16, 266, 425
Kzo9I	GATC	1 cut(s)	19
LguI	GCTCTTC	1 cut(s)	475
LmnI	GCTCC	1 cut(s)	229
LweI	GCATC	3 cut(s)	225, 319, 482
MaeI	CTAG	1 cut(s)	486
MaeII	ACGT	1 cut(s)	385
MaeIII	GTNAC	3 cut(s)	102, 185, 554
MalI	GATC	1 cut(s)	21
MboI	GATC	1 cut(s)	19
MboII	GAAGA	3 cut(s)	275, 492, 529
MfeI	CAATTG	1 cut(s)	93
MluCI	AATT	2 cut(s)	93, 577
MlyI	GAGTC	1 cut(s)	74
MmeI	TCCRAC	2 cut(s)	136, 143
MnlI	CCTC	6 cut(s)	156, 176, 258, 331, 482, 619
Mph1103I	ATGCAT	1 cut(s)	475
MseI	TTAA	3 cut(s)	290, 429, 510
MslI	CAYNNNNRTG	1 cut(s)	468
MspCI	CTTAAG	1 cut(s)	428
MspI	CCGG	1 cut(s)	536
MspR9I	CCNGG	2 cut(s)	111, 180
MunI	CAATTG	1 cut(s)	93
MvaI	CCWGG	2 cut(s)	111, 180
MvnI	CGCG	1 cut(s)	18
MwoI	GCNNNNNNNGC	3 cut(s)	388, 448, 501
NdeII	GATC	1 cut(s)	19
NlaIII	CATG	4 cut(s)	170, 374, 455, 473
NlaIV	GGNNCC	3 cut(s)	177, 342, 530
NmuCI	GTSAC	2 cut(s)	102, 554
NsiI	ATGCAT	1 cut(s)	475
NspI	RCATGY	2 cut(s)	170, 473
PaeI	GCATGC	1 cut(s)	473
PaqCI	CACCTGC	1 cut(s)	299
PciSI	GCTCTTC	1 cut(s)	475
PcsI	WCGNNNNNNNCGW	1 cut(s)	394
PfeI	GAWTC	5 cut(s)	38, 297, 410, 433, 455
PflMI	CCANNNNNTGG	1 cut(s)	155
PfoI	TCCNGGA	1 cut(s)	109
PleI	GAGTC	1 cut(s)	73
PpsI	GAGTC	1 cut(s)	73
Psp6I	CCWGG	2 cut(s)	109, 178
PspEI	GGTNACC	1 cut(s)	185
PspGI	CCWGG	2 cut(s)	109, 178
PspN4I	GGNNCC	3 cut(s)	177, 342, 530
PstNI	CAGNNNCTG	1 cut(s)	413
RsaI	GTAC	1 cut(s)	144
RsaNI	GTAC	1 cut(s)	143
RseI	CAYNNNNRTG	1 cut(s)	468
SapI	GCTCTTC	1 cut(s)	475
SaqAI	TTAA	3 cut(s)	290, 429, 510
Sau3AI	GATC	1 cut(s)	19
SchI	GAGTC	1 cut(s)	74
ScrFI	CCNGG	2 cut(s)	111, 180
SetI	ASST	9 cut(s)	91, 148, 156, 187, 313, 346, 388, 487, 603
SfaNI	GCATC	3 cut(s)	225, 319, 482
SfcI	CTRYAG	1 cut(s)	208
SmiMI	CAYNNNNRTG	1 cut(s)	468
SmlI	CTYRAG	2 cut(s)	69, 428
SmoI	CTYRAG	2 cut(s)	69, 428
SphI	GCATGC	1 cut(s)	473
Sse9I	AATT	2 cut(s)	93, 577
SsiI	CCGC	1 cut(s)	495
SspMI	CTAG	1 cut(s)	486
StyD4I	CCNGG	2 cut(s)	109, 178
TaaI	ACNGT	2 cut(s)	277, 366
TaiI	ACGT	1 cut(s)	388
TaqI	TCGA	2 cut(s)	436, 460
TasI	AATT	2 cut(s)	93, 577
TfiI	GAWTC	5 cut(s)	38, 297, 410, 433, 455
Tru1I	TTAA	3 cut(s)	290, 429, 510
Tru9I	TTAA	3 cut(s)	290, 429, 510
TscAI	CASTG	3 cut(s)	177, 324, 592
TseFI	GTSAC	2 cut(s)	102, 554
Tsp45I	GTSAC	2 cut(s)	102, 554
TspDTI	ATGAA	6 cut(s)	65, 69, 246, 269, 468, 555
TspRI	CASTG	3 cut(s)	177, 324, 592
Van91I	CCANNNNNTGG	1 cut(s)	155
Vha464I	CTTAAG	1 cut(s)	428
XceI	RCATGY	2 cut(s)	170, 473
XspI	CTAG	1 cut(s)	486
ZraI	GACGTC	1 cut(s)	386
Zsp2I	ATGCAT	1 cut(s)	475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.