AT1G30650
WRKY Family

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010362
Physical Location & Seq
Forward (+)
10868218 .. 10871297
3080 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G30650.1

Sequence Viewer

Length: 1293 bp
ATGTGCAGCGTCTCTGAGCTTCTTGACATGGAAAACTTCCAAGGAGACTTAACCGACGTCGTACGAGGAATCGGAGGCCACGTGTTATCACCGGAGACTCCTCCCTCGAACATCTGGCCTCTTCCTCTGTCACATCCAACACCATCACCGTCAGATCTTAACATAAACCCCTTCGGAGATCCCTTTGTGAGCATGGACGATCCACTCCTCCAAGAACTAAACTCCATCACAAACTCCGGCTATTTCTCCACCGTAGGAGATAACAACAACAACATTCACAACAACAATGGTTTCTTGGTTCCAAAGGTATTTGAGGAGGATCATATAAAGAGTCAATGTAGTATCTTCCCAAGAATCCGGATCTCGCATAGTAACATCATCCACGATTCTTCTCCGTGTAATTCTCCGGCCATGTCGGCTCACGTTGTCGCAGCCGCAGCAGCCGCCTCGCCGAGAGGCATCATCAACGTAGACACAAACAGTCCTAGAAACTGTCTATTGGTTGATGGTACCACGTTCTCCTCGCAGATTCAGATATCTTCCCCTCGGAATCTAGGCCTTAAAAGAAGGAAGAGTCAGGCAAAGAAGGTGGTGTGTATTCCGGCCCCGGCTGCAATGAACAGCCGATCAAGCGGAGAAGTGGTTCCATCGGATCTATGGGCTTGGCGTAAATACGGTCAAAAACCTATCAAAGGCTCTCCTTTTCCAAGGGGTTATTATAGATGCAGCAGCTCAAAAGGTTGTTCAGCAAGAAAGCAAGTCGAAAGAAGCCGAACCGATCCAAACATGTTGGTGATTACATATACCTCCGAACATAACCATCCTTGGCCCATCCAACGCAACGCTCTCGCCGGCTCCACACGCTCCTCCACCTCCTCCTCATCTAACCCTAATCCTTCCAAACCCTCAACCGCAAACGTAAACTCCTCATCCATTGGCTCCCAAAACACCATCTACTTGCCTTCCTCCACCACTCCTCCTCCTACCCTCTCATCCTCCGCCATCAAAGATGAACGAGGGGACGATATGGAGTTGGAAAACGTAGATGATGATGATGATAACCAGATTGCTCCATACAGACCGGAGCTTCATGATCATCAGCACCAACCAGATGATTTCTTTGCAGATCTTGAAGAGCTAGAAGGAGATTCTCTAAGCATGTTGCTTTCTCATGGCTGTGGCGGCGACGGGAAGGATAAAACGACCGCGTCCGATGGGATCAGCAATTTCTTCGGGTGGTCGGGAGATAATAATTATAATAATTACGACGACCAAGACTCAAGGTCGTTATAG

Protein Analysis

430

Amino Acids

46.8

Weight (kDa)

5.26

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 218 - 275 3.2e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1257
AatII GACGTC 1 cut(s) 60
Acc65I GGTACC 1 cut(s) 509
AccB1I GGYRCC 1 cut(s) 509
AccI GTMKAC 1 cut(s) 471
AccII CGCG 1 cut(s) 1208
AccIII TCCGGA 1 cut(s) 357
AciI CCGC 7 cut(s) 435, 444, 633, 912, 999, 1182, 1206
AclWI GGATC 7 cut(s) 173, 194, 327, 368, 660, 773, 1226
AcoI YGGCCR 1 cut(s) 408
AcvI CACGTG 1 cut(s) 82
AcyI GRCGYC 1 cut(s) 57
AfaI GTAC 2 cut(s) 63, 511
AfiI CCNNNNNNNGG 1 cut(s) 692
AflIII ACRYGT 2 cut(s) 81, 786
AgsI TTSAA 1 cut(s) 1133
AhdI GACNNNNNGTC 1 cut(s) 1282
AloI GAACNNNNNNTCC 2 cut(s) 627, 659
AluBI AGCT 4 cut(s) 19, 732, 1087, 1138
AluI AGCT 4 cut(s) 19, 732, 1087, 1138
Alw26I GTCTC 3 cut(s) 16, 39, 89
AlwI GGATC 7 cut(s) 173, 194, 327, 368, 660, 773, 1226
Aor13HI TCCGGA 1 cut(s) 357
AoxI GGCC 6 cut(s) 76, 116, 408, 556, 603, 827
ApeKI GCWGC 7 cut(s) 6, 431, 437, 440, 611, 726, 729
Asp700I GAANNNNTTC 1 cut(s) 642
Asp718I GGTACC 1 cut(s) 509
AspS9I GGNCC 2 cut(s) 604, 828
AsuC2I CCSGG 1 cut(s) 608
AsuHPI GGTGA 3 cut(s) 81, 138, 805
BanI GGYRCC 1 cut(s) 509
BbrPI CACGTG 1 cut(s) 82
BbvI GCAGC 7 cut(s) 18, 443, 449, 452, 598, 738, 741
BccI CCATC 9 cut(s) 151, 233, 500, 655, 828, 839, 959, 1010, 1208
BcgI CGANNNNNNTGC 6 cut(s) 429, 463, 829, 863, 1213, 1247
BclI TGATCA 1 cut(s) 1093
BcnI CCSGG 1 cut(s) 608
BcoDI GTCTC 3 cut(s) 16, 39, 89
BfaI CTAG 3 cut(s) 486, 554, 1139
BglI GCCNNNNNGGC 1 cut(s) 416
BglII AGATCT 2 cut(s) 154, 1126
Bme1390I CCNGG 1 cut(s) 608
BmeRI GACNNNNNGTC 1 cut(s) 1282
BmgT120I GGNCC 2 cut(s) 604, 828
BmiI GGNNCC 6 cut(s) 300, 511, 606, 645, 856, 940
BmrFI CCNGG 1 cut(s) 608
BmsI GCATC 2 cut(s) 468, 713
BpuEI CTTGAG 1 cut(s) 1264
BpuMI CCSGG 1 cut(s) 608
BsaAI YACGTR 1 cut(s) 82
BsaHI GRCGYC 1 cut(s) 57
BsaJI CCNNGG 5 cut(s) 40, 545, 606, 707, 824
BsaWI WCCGGW 3 cut(s) 91, 357, 1081
Bsc4I CCNNNNNNNGG 1 cut(s) 692
Bse118I RCCGGY 1 cut(s) 851
Bse3DI GCAATG 1 cut(s) 621
BseAI TCCGGA 1 cut(s) 357
BseDI CCNNGG 5 cut(s) 40, 545, 606, 707, 824
BseGI GGATG 6 cut(s) 133, 378, 820, 831, 929, 992
BseLI CCNNNNNNNGG 1 cut(s) 692
BseMI GCAATG 1 cut(s) 621
BseMII CTCAG 1 cut(s) 6
BseXI GCAGC 7 cut(s) 18, 443, 449, 452, 598, 738, 741
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 1208
Bsh1285I CGRYCG 1 cut(s) 1206
BshFI GGCC 6 cut(s) 78, 118, 410, 558, 605, 829
BshNI GGYRCC 1 cut(s) 509
BsiEI CGRYCG 1 cut(s) 1206
BsiSI CCGG 8 cut(s) 92, 237, 358, 407, 602, 608, 852, 1082
BsiWI CGTACG 1 cut(s) 61
BslFI GGGAC 1 cut(s) 1034
BslI CCNNNNNNNGG 1 cut(s) 692
BsmAI GTCTC 3 cut(s) 16, 39, 89
BsmBI CGTCTC 1 cut(s) 16
BsmFI GGGAC 1 cut(s) 1034
BsnI GGCC 6 cut(s) 78, 118, 410, 558, 605, 829
Bsp13I TCCGGA 1 cut(s) 357
BspACI CCGC 7 cut(s) 435, 444, 633, 912, 999, 1182, 1206
BspANI GGCC 6 cut(s) 78, 118, 410, 558, 605, 829
BspCNI CTCAG 1 cut(s) 7
BspEI TCCGGA 1 cut(s) 357
BspFNI CGCG 1 cut(s) 1208
BspHI TCATGA 1 cut(s) 1090
BspLI GGNNCC 6 cut(s) 300, 511, 606, 645, 856, 940
BspPI GGATC 7 cut(s) 173, 194, 327, 368, 660, 773, 1226
BspQI GCTCTTC 1 cut(s) 1128
BspT107I GGYRCC 1 cut(s) 509
BsrDI GCAATG 1 cut(s) 621
BsrFI RCCGGY 1 cut(s) 851
BssAI RCCGGY 1 cut(s) 851
BssECI CCNNGG 5 cut(s) 40, 545, 606, 707, 824
BssNI GRCGYC 1 cut(s) 57
BssT1I CCWWGG 3 cut(s) 40, 707, 824
Bst4CI ACNGT 5 cut(s) 150, 253, 482, 494, 677
Bst6I CTCTTC 3 cut(s) 126, 566, 1128
BstACI GRCGYC 1 cut(s) 57
BstBAI YACGTR 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 853
BstDEI CTNAG 2 cut(s) 15, 1154
BstENI CCTNNNNNAGG 1 cut(s) 690
BstF5I GGATG 6 cut(s) 133, 378, 820, 831, 929, 992
BstFNI CGCG 1 cut(s) 1208
BstMAI GTCTC 3 cut(s) 16, 39, 89
BstMCI CGRYCG 1 cut(s) 1206
BstMWI GCNNNNNNNGC 8 cut(s) 416, 437, 440, 443, 611, 630, 861, 1182
BstNSI RCATGY 2 cut(s) 790, 1162
BstSCI CCNGG 1 cut(s) 606
BstUI CGCG 1 cut(s) 1208
BstV1I GCAGC 7 cut(s) 18, 443, 449, 452, 598, 738, 741
BstX2I RGATCY 5 cut(s) 154, 178, 360, 652, 1126
BstYI RGATCY 5 cut(s) 154, 178, 360, 652, 1126
BsuRI GGCC 6 cut(s) 78, 118, 410, 558, 605, 829
BtsCI GGATG 6 cut(s) 133, 378, 820, 831, 929, 992
Cac8I GCNNGC 1 cut(s) 853
CciI TCATGA 1 cut(s) 1090
Cfr10I RCCGGY 1 cut(s) 851
Cfr13I GGNCC 2 cut(s) 604, 828
CseI GACGC 1 cut(s) 1197
Csp6I GTAC 2 cut(s) 62, 510
CspCI CAANNNNNGTGG 2 cut(s) 570, 605
CviAII CATG 7 cut(s) 28, 193, 412, 787, 1091, 1159, 1172
CviQI GTAC 2 cut(s) 62, 510
DdeI CTNAG 2 cut(s) 15, 1154
DriI GACNNNNNGTC 1 cut(s) 1282
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 3 cut(s) 126, 566, 1128
Eam1105I GACNNNNNGTC 1 cut(s) 1282
EarI CTCTTC 3 cut(s) 126, 566, 1128
EciI GGCGGA 1 cut(s) 988
Eco130I CCWWGG 3 cut(s) 40, 707, 824
Eco147I AGGCCT 1 cut(s) 558
Eco32I GATATC 1 cut(s) 537
Eco72I CACGTG 1 cut(s) 82
EcoNI CCTNNNNNAGG 1 cut(s) 690
EcoRV GATATC 1 cut(s) 537
EcoT14I CCWWGG 3 cut(s) 40, 707, 824
ErhI CCWWGG 3 cut(s) 40, 707, 824
Esp3I CGTCTC 1 cut(s) 16
FaeI CATG 7 cut(s) 31, 196, 415, 790, 1094, 1162, 1175
FaqI GGGAC 1 cut(s) 1034
FatI CATG 7 cut(s) 27, 192, 411, 786, 1090, 1158, 1171
FbaI TGATCA 1 cut(s) 1093
FblI GTMKAC 1 cut(s) 471
FokI GGATG 6 cut(s) 120, 365, 807, 818, 916, 979
FspBI CTAG 3 cut(s) 486, 554, 1139
HaeIII GGCC 6 cut(s) 78, 118, 410, 558, 605, 829
HapII CCGG 8 cut(s) 92, 237, 358, 407, 602, 608, 852, 1082
HgaI GACGC 1 cut(s) 1197
Hin1I GRCGYC 1 cut(s) 57
Hin1II CATG 7 cut(s) 31, 196, 415, 790, 1094, 1162, 1175
HpaII CCGG 8 cut(s) 92, 237, 358, 407, 602, 608, 852, 1082
HphI GGTGA 3 cut(s) 81, 138, 805
Hpy166II GTNNAC 2 cut(s) 472, 922
Hpy188I TCNGA 9 cut(s) 16, 74, 154, 176, 534, 549, 652, 811, 1213
Hpy188III TCNNGA 5 cut(s) 23, 358, 1091, 1130, 1242
Hpy8I GTNNAC 2 cut(s) 472, 922
Hpy99I CGWCG 4 cut(s) 59, 62, 1190, 1271
HpyAV CCTTC 7 cut(s) 181, 561, 580, 906, 972, 1136, 1186
HpyCH4III ACNGT 5 cut(s) 150, 253, 482, 494, 677
HpyCH4IV ACGT 7 cut(s) 57, 81, 423, 468, 515, 918, 1041
HpyCH4V TGCA 4 cut(s) 6, 614, 726, 1124
HpyF10VI GCNNNNNNNGC 8 cut(s) 416, 437, 440, 443, 611, 630, 861, 1182
HpyF3I CTNAG 2 cut(s) 15, 1154
HpySE526I ACGT 7 cut(s) 57, 81, 423, 468, 515, 918, 1041
Hsp92I GRCGYC 1 cut(s) 57
Hsp92II CATG 7 cut(s) 31, 196, 415, 790, 1094, 1162, 1175
Kpn2I TCCGGA 1 cut(s) 357
KpnI GGTACC 1 cut(s) 513
KroI GCCGGC 1 cut(s) 851
KroNI GCCGGC 1 cut(s) 853
Ksp22I TGATCA 1 cut(s) 1093
LguI GCTCTTC 1 cut(s) 1128
LmnI GCTCC 5 cut(s) 860, 869, 944, 1075, 1084
Lsp1109I GCAGC 7 cut(s) 18, 443, 449, 452, 598, 738, 741
LweI GCATC 2 cut(s) 468, 713
MaeI CTAG 3 cut(s) 486, 554, 1139
MaeII ACGT 7 cut(s) 57, 81, 423, 468, 515, 918, 1041
MaeIII GTNAC 2 cut(s) 129, 371
MboII GAAGA 7 cut(s) 113, 337, 381, 531, 583, 1145, 1222
MflI RGATCY 5 cut(s) 154, 178, 360, 652, 1126
MluCI AATT 4 cut(s) 400, 1225, 1252, 1261
MlyI GAGTC 4 cut(s) 91, 340, 583, 1271
MmeI TCCRAC 3 cut(s) 161, 859, 1014
MroI TCCGGA 1 cut(s) 357
MroNI GCCGGC 1 cut(s) 851
MroXI GAANNNNTTC 1 cut(s) 642
MseI TTAA 3 cut(s) 50, 159, 561
MslI CAYNNNNRTG 2 cut(s) 791, 1176
MspI CCGG 8 cut(s) 92, 237, 358, 407, 602, 608, 852, 1082
MspR9I CCNGG 1 cut(s) 608
MvnI CGCG 1 cut(s) 1208
MwoI GCNNNNNNNGC 8 cut(s) 416, 437, 440, 443, 611, 630, 861, 1182
NaeI GCCGGC 1 cut(s) 853
NciI CCSGG 1 cut(s) 608
NgoMIV GCCGGC 1 cut(s) 851
NlaIII CATG 7 cut(s) 31, 196, 415, 790, 1094, 1162, 1175
NlaIV GGNNCC 6 cut(s) 300, 511, 606, 645, 856, 940
NmeAIII GCCGAG 1 cut(s) 477
NmuCI GTSAC 1 cut(s) 129
NspI RCATGY 2 cut(s) 790, 1162
PagI TCATGA 1 cut(s) 1090
PceI AGGCCT 1 cut(s) 558
PciI ACATGT 1 cut(s) 786
PciSI GCTCTTC 1 cut(s) 1128
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PdiI GCCGGC 1 cut(s) 853
PdmI GAANNNNTTC 1 cut(s) 642
PfeI GAWTC 6 cut(s) 69, 354, 386, 529, 550, 1148
Pfl23II CGTACG 1 cut(s) 61
PflFI GACNNNGTC 1 cut(s) 1207
PleI GAGTC 4 cut(s) 91, 339, 582, 1271
PmaCI CACGTG 1 cut(s) 82
PmlI CACGTG 1 cut(s) 82
PpsI GAGTC 4 cut(s) 91, 339, 582, 1271
Ppu21I YACGTR 1 cut(s) 82
PscI ACATGT 1 cut(s) 786
PsiI TTATAA 1 cut(s) 1257
PspCI CACGTG 1 cut(s) 82
PspLI CGTACG 1 cut(s) 61
PspN4I GGNNCC 6 cut(s) 300, 511, 606, 645, 856, 940
PspPI GGNCC 2 cut(s) 604, 828
PsuI RGATCY 5 cut(s) 154, 178, 360, 652, 1126
PsyI GACNNNGTC 1 cut(s) 1207
RsaI GTAC 2 cut(s) 63, 511
RsaNI GTAC 2 cut(s) 62, 510
RseI CAYNNNNRTG 2 cut(s) 791, 1176
SapI GCTCTTC 1 cut(s) 1128
SaqAI TTAA 3 cut(s) 50, 159, 561
Sau96I GGNCC 2 cut(s) 604, 828
SchI GAGTC 4 cut(s) 91, 340, 583, 1271
ScrFI CCNGG 1 cut(s) 608
SfaNI GCATC 2 cut(s) 468, 713
SmiMI CAYNNNNRTG 2 cut(s) 791, 1176
SmlI CTYRAG 1 cut(s) 1279
SmoI CTYRAG 1 cut(s) 1279
Sse9I AATT 4 cut(s) 400, 1225, 1252, 1261
SseBI AGGCCT 1 cut(s) 558
SsiI CCGC 7 cut(s) 435, 444, 633, 912, 999, 1182, 1206
SspMI CTAG 3 cut(s) 486, 554, 1139
StuI AGGCCT 1 cut(s) 558
StyD4I CCNGG 1 cut(s) 606
StyI CCWWGG 3 cut(s) 40, 707, 824
TaaI ACNGT 5 cut(s) 150, 253, 482, 494, 677
TaiI ACGT 7 cut(s) 60, 84, 426, 471, 518, 921, 1044
TaqI TCGA 2 cut(s) 107, 762
TasI AATT 4 cut(s) 400, 1225, 1252, 1261
TauI GCSGC 3 cut(s) 437, 446, 1185
TfiI GAWTC 6 cut(s) 69, 354, 386, 529, 550, 1148
Tru1I TTAA 3 cut(s) 50, 159, 561
Tru9I TTAA 3 cut(s) 50, 159, 561
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 7 cut(s) 6, 431, 437, 440, 611, 726, 729
Tsp45I GTSAC 1 cut(s) 129
TspDTI ATGAA 3 cut(s) 632, 1026, 1079
TspGWI ACGGA 1 cut(s) 384
Tth111I GACNNNGTC 1 cut(s) 1207
XagI CCTNNNNNAGG 1 cut(s) 690
XceI RCATGY 2 cut(s) 790, 1162
XcmI CCANNNNNNNNNTGG 1 cut(s) 654
XmiI GTMKAC 1 cut(s) 471
XmnI GAANNNNTTC 1 cut(s) 642
XspI CTAG 3 cut(s) 486, 554, 1139
ZraI GACGTC 1 cut(s) 58
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.