MD05G1265200.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010362
Physical Location & Seq
Forward (+)
40011060 .. 40015621
4562 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1265200.v1.1.491

Sequence Viewer

Length: 1479 bp
ATGTGCAGCTTGTTCATGCCAACTACTAGGATGGAGAATTTTCAAGGTGATTTAACCGACATACTAAGAGCTGGTACTACTACTGCTACCGTTGGAGCTGATCCGTCTGATCATCAAGAACCTTACACCGACTCGTGGCACTTTCCATCTGATCATCGCATGATATTCCCATCATCCTCATCAGCCATGGAAGAAGCAGAAGAAACTAAGGACAACTTTGGCGATCCATTTTTGTCGTACATGCGCGATCCGCTGCTTAACGAGCTAGGCATATCAAGTTCCAGCCTTTTCAGCAGCCCCAATTGTACAGCTCGTGCGGAGGAAGATGTTACAACTACTACTTTTGGTAGCGGTGGCGATGGCAGTCATCATGTACTTAGTCTTGGGCATCACAACCATCAAAGTGGCGGTGTTGTGGTTGATGGTGATCCAATAAAGAGGCCTTACAACATATTCTCACGGATGCTACAGATCTCTCCGAGTGCAAAGGTACCGGTGTCGCCATGTGATTCTCCGGTGATAGGTGCAGCAGTGTCGTCGTCTCCTAGGGAGATTAAAACATCATCAGCTTCTACTGCCATGGTTGCAAGCGACATGATTATTAATGCGAACAGCTCATCAGCAGCAAAAGGTTGCTTGCTCGAGAACACTGGGATGCAGATCTCATCTCCACGGAATCCGGGTATCAAGCGAAGGAAGAGCCAGGCAAAGAAGGTGGTGTGCATTCCGGCGCCAGCGGCTGCAAACAGCAGGCCTACTGGAGAAGTAGTTCCGTCTGATCTGTGGGCATGGAGAAAGTACGGTCAGAAACCCATCAAAGGGTCACCTTATCCAAGGGGTTACTATAGATGTAGCAGCTCAAAGGGTTGCTCAGCAAGGAAACAAGTAGAGCGTAGCCGGACGGATCCAAAAATGTTGGTCATCACGTACACTTCTGAACACAACCATCCATGGCCTACTCAGAGAAATGCCCTAGCTGGCTCAACCAGATCCCAGCCATCCAAAAATGGTGGCGCCGCTGCCTTGAAGATCTCTCCCAATAGCTCTCATCAGCCTCATCAAAAGCCAACTGGCCCAACAAAGGAAGAACAAAATGAGAATGTGAATGATAATGATGATAATCATATGTCTCCAACAGTTATTACCGCTGGAAGTGCTTCAACAGTGAAGGAAGAATTTGAGGACGTTGAAAAGCAATTAGAGCTTGATCATCATCATCATCAGCAATTAGGGTTTGCTTATAGACCATCTATGCCTGATGAGCAGTCCAACCAGTCACACCATCTTGATGACTTCTTTGCTGATTTAGGAGAAATTGAGGCTGACCCACTTAACCTCTTGTTTTTACAATGTTTCTCGGCAGATGAGCAGCAGCAAAAGGCTGCAGAGCCCTTAGATCCATTCAATCTCTTAGATAATTGGTCAGGAGATCACAACACCAACAATACTTCATTTGCTGAAGCTAAGAGGCGGTTATAA

Protein Analysis

493

Amino Acids

53.78

Weight (kDa)

5.85

Isoelectric Point (pI)

56.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 260 - 317 4.9e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1477
Acc65I GGTACC 1 cut(s) 490
AccB1I GGYRCC 3 cut(s) 490, 730, 1013
AccII CGCG 1 cut(s) 246
AciI CCGC 8 cut(s) 251, 317, 351, 408, 737, 1017, 1146, 1471
AclWI GGATC 8 cut(s) 95, 218, 242, 422, 899, 912, 984, 1391
AcsI RAATTY 2 cut(s) 37, 1175
AcuI CTGAAG 1 cut(s) 1479
AcyI GRCGYC 2 cut(s) 731, 1014
AfaI GTAC 7 cut(s) 76, 239, 307, 375, 492, 800, 929
AfiI CCNNNNNNNGG 5 cut(s) 135, 521, 818, 819, 1081
AgeI ACCGGT 1 cut(s) 493
AgsI TTSAA 5 cut(s) 44, 1027, 1161, 1190, 1405
AjnI CCWGG 1 cut(s) 702
AloI GAACNNNNNNTCC 2 cut(s) 753, 785
Alw26I GTCTC 2 cut(s) 546, 1134
AlwI GGATC 8 cut(s) 95, 218, 242, 422, 899, 912, 984, 1391
AlwNI CAGNNNCTG 1 cut(s) 740
Ama87I CYCGRG 1 cut(s) 641
AoxI GGCC 4 cut(s) 440, 752, 953, 1072
ApoI RAATTY 2 cut(s) 37, 1175
AseI ATTAAT 1 cut(s) 603
AsiGI ACCGGT 1 cut(s) 493
Asp700I GAANNNNTTC 2 cut(s) 768, 1156
Asp718I GGTACC 1 cut(s) 490
AspA2I CCTAGG 1 cut(s) 545
AspLEI GCGC 3 cut(s) 246, 733, 1016
AspS9I GGNCC 1 cut(s) 1073
AsuC2I CCSGG 1 cut(s) 681
AsuHPI GGTGA 4 cut(s) 59, 437, 529, 816
AvaI CYCGRG 1 cut(s) 641
AvrII CCTAGG 1 cut(s) 545
BaeI ACNNNNGTAYC 2 cut(s) 482, 515
BamHI GGATCC 1 cut(s) 904
BanI GGYRCC 3 cut(s) 490, 730, 1013
BanII GRGCYC 1 cut(s) 1392
BauI CACGAG 2 cut(s) 133, 312
BcgI CGANNNNNNTGC 4 cut(s) 516, 519, 550, 553
BciT130I CCWGG 1 cut(s) 704
BclI TGATCA 3 cut(s) 109, 151, 1207
BcnI CCSGG 1 cut(s) 681
BcoDI GTCTC 2 cut(s) 546, 1134
BfaI CTAG 4 cut(s) 27, 266, 546, 974
BfmI CTRYAG 3 cut(s) 467, 844, 1383
BfoI RGCGCY 2 cut(s) 734, 1017
BglII AGATCT 3 cut(s) 471, 660, 1029
BlnI CCTAGG 1 cut(s) 545
BlpI GCTNAGC 1 cut(s) 871
Bme1390I CCNGG 2 cut(s) 681, 704
BmeT110I CYCGRG 1 cut(s) 641
BmgT120I GGNCC 1 cut(s) 1073
BmiI GGNNCC 4 cut(s) 492, 732, 906, 1015
BmrFI CCNGG 2 cut(s) 681, 704
BmrI ACTGGG 1 cut(s) 660
BmsI GCATC 3 cut(s) 397, 453, 645
BmuI ACTGGG 1 cut(s) 660
BpmI CTGGAG 1 cut(s) 780
Bpu1102I GCTNAGC 1 cut(s) 871
BpuMI CCSGG 1 cut(s) 681
BsaAI YACGTR 1 cut(s) 927
BsaBI GATNNNNATC 4 cut(s) 426, 659, 1119, 1212
BsaHI GRCGYC 2 cut(s) 731, 1014
BsaJI CCNNGG 6 cut(s) 186, 545, 579, 671, 833, 950
BsaWI WCCGGW 2 cut(s) 493, 514
BsaXI ACNNNNNCTCC 2 cut(s) 753, 783
Bsc4I CCNNNNNNNGG 5 cut(s) 135, 521, 818, 819, 1081
Bse118I RCCGGY 1 cut(s) 493
Bse1I ACTGG 4 cut(s) 655, 763, 1075, 1273
Bse8I GATNNNNATC 4 cut(s) 426, 659, 1119, 1212
BseBI CCWGG 1 cut(s) 704
BseDI CCNNGG 6 cut(s) 186, 545, 579, 671, 833, 950
BseGI GGATG 6 cut(s) 36, 173, 468, 660, 946, 998
BseJI GATNNNNATC 4 cut(s) 426, 659, 1119, 1212
BseLI CCNNNNNNNGG 5 cut(s) 135, 521, 818, 819, 1081
BseMII CTCAG 2 cut(s) 885, 974
BseNI ACTGG 4 cut(s) 655, 763, 1075, 1273
BseYI CCCAGC 1 cut(s) 993
BsgI GTGCAG 2 cut(s) 25, 546
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 4 cut(s) 442, 754, 955, 1074
BshNI GGYRCC 3 cut(s) 490, 730, 1013
BshTI ACCGGT 1 cut(s) 493
BsiHKCI CYCGRG 1 cut(s) 641
BsiSI CCGG 5 cut(s) 494, 515, 680, 728, 898
BslI CCNNNNNNNGG 5 cut(s) 135, 521, 818, 819, 1081
BsmAI GTCTC 2 cut(s) 546, 1134
BsmBI CGTCTC 1 cut(s) 546
BsmI GAATGC 1 cut(s) 723
BsnI GGCC 4 cut(s) 442, 754, 955, 1074
BsoBI CYCGRG 1 cut(s) 641
Bsp1286I GDGCHC 1 cut(s) 1392
Bsp1407I TGTACA 1 cut(s) 305
Bsp1720I GCTNAGC 1 cut(s) 871
Bsp19I CCATGG 3 cut(s) 186, 579, 950
BspACI CCGC 8 cut(s) 251, 317, 351, 408, 737, 1017, 1146, 1471
BspANI GGCC 4 cut(s) 442, 754, 955, 1074
BspCNI CTCAG 2 cut(s) 884, 973
BspFNI CGCG 1 cut(s) 246
BspLI GGNNCC 4 cut(s) 492, 732, 906, 1015
BspMAI CTGCAG 1 cut(s) 1387
BspPI GGATC 8 cut(s) 95, 218, 242, 422, 899, 912, 984, 1391
BspQI GCTCTTC 1 cut(s) 692
BspT107I GGYRCC 3 cut(s) 490, 730, 1013
BsrFI RCCGGY 1 cut(s) 493
BsrGI TGTACA 1 cut(s) 305
BsrI ACTGG 4 cut(s) 655, 763, 1075, 1273
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 6 cut(s) 186, 545, 579, 671, 833, 950
BssNI GRCGYC 2 cut(s) 731, 1014
BssSI CACGAG 2 cut(s) 133, 312
BssT1I CCWWGG 5 cut(s) 186, 545, 579, 833, 950
Bst2BI CACGAG 2 cut(s) 133, 312
Bst2UI CCWGG 1 cut(s) 704
Bst4CI ACNGT 4 cut(s) 91, 803, 1138, 1165
Bst6I CTCTTC 1 cut(s) 692
BstACI GRCGYC 2 cut(s) 731, 1014
BstAUI TGTACA 1 cut(s) 305
BstBAI YACGTR 1 cut(s) 927
BstC8I GCNNGC 5 cut(s) 589, 638, 735, 752, 979
BstDEI CTNAG 8 cut(s) 65, 207, 377, 871, 960, 1393, 1411, 1464
BstDSI CCRYGG 4 cut(s) 186, 579, 671, 950
BstEII GGTNACC 1 cut(s) 822
BstF5I GGATG 6 cut(s) 36, 173, 468, 660, 946, 998
BstFNI CGCG 1 cut(s) 246
BstH2I RGCGCY 2 cut(s) 734, 1017
BstHHI GCGC 3 cut(s) 246, 733, 1016
BstMAI GTCTC 2 cut(s) 546, 1134
BstMWI GCNNNNNNNGC 9 cut(s) 250, 262, 291, 575, 584, 737, 1154, 1201, 1261
BstNI CCWGG 1 cut(s) 704
BstNSI RCATGY 1 cut(s) 244
BstPI GGTNACC 1 cut(s) 822
BstSCI CCNGG 2 cut(s) 679, 702
BstSFI CTRYAG 3 cut(s) 467, 844, 1383
BstUI CGCG 1 cut(s) 246
BstX2I RGATCY 6 cut(s) 471, 660, 904, 989, 1029, 1396
BstXI CCANNNNNNTGG 1 cut(s) 404
BstYI RGATCY 6 cut(s) 471, 660, 904, 989, 1029, 1396
BsuRI GGCC 4 cut(s) 442, 754, 955, 1074
BtgI CCRYGG 4 cut(s) 186, 579, 671, 950
BtgZI GCGATG 2 cut(s) 140, 372
BtsCI GGATG 6 cut(s) 36, 173, 468, 660, 946, 998
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 3 cut(s) 537, 648, 1170
Cac8I GCNNGC 5 cut(s) 589, 638, 735, 752, 979
CaiI CAGNNNCTG 1 cut(s) 740
CfoI GCGC 3 cut(s) 246, 733, 1016
Cfr10I RCCGGY 1 cut(s) 493
Cfr13I GGNCC 1 cut(s) 1073
Csp6I GTAC 7 cut(s) 75, 238, 306, 374, 491, 799, 928
CspAI ACCGGT 1 cut(s) 493
CspCI CAANNNNNGTGG 4 cut(s) 696, 731, 991, 1026
CviQI GTAC 7 cut(s) 75, 238, 306, 374, 491, 799, 928
DdeI CTNAG 8 cut(s) 65, 207, 377, 871, 960, 1393, 1411, 1464
DinI GGCGCC 2 cut(s) 732, 1015
Eam1104I CTCTTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 692
Eco130I CCWWGG 5 cut(s) 186, 545, 579, 833, 950
Eco147I AGGCCT 2 cut(s) 442, 754
Eco24I GRGCYC 1 cut(s) 1392
Eco57I CTGAAG 1 cut(s) 1479
Eco88I CYCGRG 1 cut(s) 641
Eco91I GGTNACC 1 cut(s) 822
EcoO65I GGTNACC 1 cut(s) 822
EcoRII CCWGG 1 cut(s) 702
EcoT14I CCWWGG 5 cut(s) 186, 545, 579, 833, 950
EcoT38I GRGCYC 1 cut(s) 1392
EgeI GGCGCC 2 cut(s) 732, 1015
EheI GGCGCC 2 cut(s) 732, 1015
ErhI CCWWGG 5 cut(s) 186, 545, 579, 833, 950
Esp3I CGTCTC 1 cut(s) 546
FalI AAGNNNNNCTT 2 cut(s) 200, 232
FauNDI CATATG 1 cut(s) 1125
FbaI TGATCA 3 cut(s) 109, 151, 1207
FokI GGATG 6 cut(s) 43, 160, 475, 667, 933, 985
FriOI GRGCYC 1 cut(s) 1392
FspBI CTAG 4 cut(s) 27, 266, 546, 974
GlaI GCGC 3 cut(s) 245, 732, 1015
GsaI CCCAGC 1 cut(s) 997
GsuI CTGGAG 1 cut(s) 780
HaeII RGCGCY 2 cut(s) 734, 1017
HaeIII GGCC 4 cut(s) 442, 754, 955, 1074
HapII CCGG 5 cut(s) 494, 515, 680, 728, 898
HhaI GCGC 3 cut(s) 246, 733, 1016
Hin1I GRCGYC 2 cut(s) 731, 1014
Hin6I GCGC 3 cut(s) 244, 731, 1014
HinP1I GCGC 3 cut(s) 244, 731, 1014
HinfI GANTC 3 cut(s) 131, 509, 676
HpaII CCGG 5 cut(s) 494, 515, 680, 728, 898
HphI GGTGA 4 cut(s) 59, 437, 529, 816
Hpy166II GTNNAC 1 cut(s) 930
Hpy188I TCNGA 7 cut(s) 109, 151, 480, 778, 807, 937, 963
Hpy188III TCNNGA 4 cut(s) 116, 643, 1286, 1425
Hpy8I GTNNAC 1 cut(s) 930
Hpy99I CGWCG 1 cut(s) 541
HpyAV CCTTC 3 cut(s) 687, 706, 1162
HpyCH4III ACNGT 4 cut(s) 91, 803, 1138, 1165
HpyCH4IV ACGT 2 cut(s) 926, 1185
HpyCH4V TGCA 8 cut(s) 6, 485, 527, 587, 658, 723, 743, 1385
HpyF10VI GCNNNNNNNGC 9 cut(s) 250, 262, 291, 575, 584, 737, 1154, 1201, 1261
HpyF3I CTNAG 8 cut(s) 65, 207, 377, 871, 960, 1393, 1411, 1464
HpySE526I ACGT 2 cut(s) 926, 1185
Hsp92I GRCGYC 2 cut(s) 731, 1014
HspAI GCGC 3 cut(s) 244, 731, 1014
KasI GGCGCC 2 cut(s) 730, 1013
KpnI GGTACC 1 cut(s) 494
Ksp22I TGATCA 3 cut(s) 109, 151, 1207
LguI GCTCTTC 1 cut(s) 692
LmnI GCTCC 1 cut(s) 95
LweI GCATC 3 cut(s) 397, 453, 645
MaeI CTAG 4 cut(s) 27, 266, 546, 974
MaeII ACGT 2 cut(s) 926, 1185
MaeIII GTNAC 4 cut(s) 328, 822, 839, 1275
MboII GAAGA 7 cut(s) 203, 212, 335, 709, 1039, 1097, 1184
MfeI CAATTG 1 cut(s) 301
MflI RGATCY 6 cut(s) 471, 660, 904, 989, 1029, 1396
MhlI GDGCHC 1 cut(s) 1392
MluCI AATT 7 cut(s) 37, 301, 1175, 1196, 1226, 1314, 1417
Mly113I GGCGCC 2 cut(s) 731, 1014
MlyI GAGTC 1 cut(s) 125
MmeI TCCRAC 3 cut(s) 73, 1157, 1293
MnlI CCTC 8 cut(s) 187, 313, 432, 1065, 1174, 1312, 1346, 1461
MroXI GAANNNNTTC 2 cut(s) 768, 1156
MseI TTAA 5 cut(s) 53, 258, 555, 603, 1332
MslI CAYNNNNRTG 3 cut(s) 402, 653, 1287
MspA1I CMGCKG 4 cut(s) 253, 737, 1019, 1148
MspI CCGG 5 cut(s) 494, 515, 680, 728, 898
MspR9I CCNGG 2 cut(s) 681, 704
MunI CAATTG 1 cut(s) 301
Mva1269I GAATGC 1 cut(s) 723
MvaI CCWGG 1 cut(s) 704
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 9 cut(s) 250, 262, 291, 575, 584, 737, 1154, 1201, 1261
NarI GGCGCC 2 cut(s) 731, 1014
NciI CCSGG 1 cut(s) 681
NcoI CCATGG 3 cut(s) 186, 579, 950
NdeI CATATG 1 cut(s) 1125
NlaIV GGNNCC 4 cut(s) 492, 732, 906, 1015
NmeAIII GCCGAG 1 cut(s) 1337
NmuCI GTSAC 2 cut(s) 822, 1275
NspI RCATGY 1 cut(s) 244
PaeR7I CTCGAG 1 cut(s) 641
PceI AGGCCT 2 cut(s) 442, 754
PciSI GCTCTTC 1 cut(s) 692
PctI GAATGC 1 cut(s) 723
PdmI GAANNNNTTC 2 cut(s) 768, 1156
PfeI GAWTC 2 cut(s) 509, 676
PinAI ACCGGT 1 cut(s) 493
PleI GAGTC 1 cut(s) 125
PluTI GGCGCC 2 cut(s) 734, 1017
PpsI GAGTC 1 cut(s) 125
Ppu21I YACGTR 1 cut(s) 927
PshBI ATTAAT 1 cut(s) 603
PsiI TTATAA 1 cut(s) 1477
Psp6I CCWGG 1 cut(s) 702
PspEI GGTNACC 1 cut(s) 822
PspFI CCCAGC 1 cut(s) 993
PspGI CCWGG 1 cut(s) 702
PspN4I GGNNCC 4 cut(s) 492, 732, 906, 1015
PspPI GGNCC 1 cut(s) 1073
PstI CTGCAG 1 cut(s) 1387
PstNI CAGNNNCTG 1 cut(s) 740
PsuI RGATCY 6 cut(s) 471, 660, 904, 989, 1029, 1396
RsaI GTAC 7 cut(s) 76, 239, 307, 375, 492, 800, 929
RsaNI GTAC 7 cut(s) 75, 238, 306, 374, 491, 799, 928
RseI CAYNNNNRTG 3 cut(s) 402, 653, 1287
SapI GCTCTTC 1 cut(s) 692
SaqAI TTAA 5 cut(s) 53, 258, 555, 603, 1332
Sau96I GGNCC 1 cut(s) 1073
SchI GAGTC 1 cut(s) 125
ScrFI CCNGG 2 cut(s) 681, 704
SduI GDGCHC 1 cut(s) 1392
SfaNI GCATC 3 cut(s) 397, 453, 645
SfcI CTRYAG 3 cut(s) 467, 844, 1383
SfoI GGCGCC 2 cut(s) 732, 1015
Sfr274I CTCGAG 1 cut(s) 641
SlaI CTCGAG 1 cut(s) 641
SmiMI CAYNNNNRTG 3 cut(s) 402, 653, 1287
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 7 cut(s) 37, 301, 1175, 1196, 1226, 1314, 1417
SseBI AGGCCT 2 cut(s) 442, 754
SsiI CCGC 8 cut(s) 251, 317, 351, 408, 737, 1017, 1146, 1471
SspDI GGCGCC 2 cut(s) 730, 1013
SspMI CTAG 4 cut(s) 27, 266, 546, 974
StuI AGGCCT 2 cut(s) 442, 754
StyD4I CCNGG 2 cut(s) 679, 702
StyI CCWWGG 5 cut(s) 186, 545, 579, 833, 950
TaaI ACNGT 4 cut(s) 91, 803, 1138, 1165
TaiI ACGT 2 cut(s) 929, 1188
TaqI TCGA 1 cut(s) 642
TasI AATT 7 cut(s) 37, 301, 1175, 1196, 1226, 1314, 1417
TatI WGTACW 2 cut(s) 305, 373
TauI GCSGC 2 cut(s) 740, 1019
TfiI GAWTC 2 cut(s) 509, 676
Tru1I TTAA 5 cut(s) 53, 258, 555, 603, 1332
Tru9I TTAA 5 cut(s) 53, 258, 555, 603, 1332
TscAI CASTG 3 cut(s) 537, 655, 1170
TseFI GTSAC 2 cut(s) 822, 1275
Tsp45I GTSAC 2 cut(s) 822, 1275
TspDTI ATGAA 2 cut(s) 4, 1440
TspGWI ACGGA 5 cut(s) 93, 475, 688, 762, 917
TspRI CASTG 3 cut(s) 537, 655, 1170
VspI ATTAAT 1 cut(s) 603
XapI RAATTY 2 cut(s) 37, 1175
XceI RCATGY 1 cut(s) 244
XhoI CTCGAG 1 cut(s) 641
XmaJI CCTAGG 1 cut(s) 545
XmnI GAANNNNTTC 2 cut(s) 768, 1156
XspI CTAG 4 cut(s) 27, 266, 546, 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.