RchiOBHm_Chr5g0018041
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010362
Physical Location & Seq
Forward (+)
12584859 .. 12588279
3421 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29826

Sequence Viewer

Length: 1491 bp
ATGTGCAGCTTGTCTATGCCAATTGGGATGGAGAATTATCAAGGCGATTTAACTGACATACTTCGAGCTGGTGCTGCTACAGGATCATCCTCATCATCGGCTTATCATTCCGAAACAATGAATTCCGACAGCTGGCAGTTTTCATCCGATGATAACTATTCTATGAAATTCTCGACATCAATGGCCATGGAAGAAGTAGAAGACGCAAGGGACAATTTCGGCGATCCGTTTTCATCGTACATACGCGATCCACTTCTTCACGAGCTGTCGTCCACATCCACCGGCGGCTTTTTCAGCAGCCCAAATTCGACTCCAGAAATGAACATCAGTGTAGATATGGAAGCTCGTGCTGCGCCGTTTGGTGCTCATGTACCTGGTCACCATCATCAAGGTGAGGTTACTAAGAGGCCTTGCAACAACATATTCTCTCGTATGCTTCAGATCTCCCCCGGGAAGCTAGGCGGCGACGTGGCGGGGTGTGATTCGGTGGCGGTGTTGGGGTCACCGAGGGGGGGAGGAGGAGGAGGAGGAATCAAGGCTGGGCCTGGTGGTGTTATGGTTGGAAGCGACATGATTAATGCAAACAGCTCAAAAGGTTGCTTGCTCGAGAACACCGGAGTTGGGGTGCAGATCTCATCTCCACGGAATCCGGGTATCAAGCGAAGGAAGAGCCAGGCGAAGAAGGTGGTATGTATTCCAGCGCCAGCAGCAGCAAACAGTAGGCCAACTGGAGAAGTAGTTCCATCTGATCTGTGGGCATGGAGAAAGTACGGTCAGAAACCCATCAAAGGTTCACCTTATCCAAGAGGTTACTATAGATGCAGCAGCTCAAAGGGTTGCTCAGCAAGGAAACAAGTAGAGCGTAGCCGGACTGATCCAAACATGTTGGTCATCACCTACACTTCCGAGCACAACCATCCCTGGCCAACCCAGAGAAATGCTCTCGCCGGCTCAACCCGATCCCAGCCATCAAAAGCCGGTGCCGGTTCAAAGACCTCTCCTAATACTGCTCATCACCAAAAACCAGATAGCCCAGCAAAGGAAGAACAAAAGGCTGCAGCTGACAGCATGAACGAGAATAATCATGTACTGTCTCCAGTTAATGCAACTGTTGGAAGTAGTTCAACAGTTAAAGAAGAGTTTGAGGACATTGAGAAGCTGCAATTGGAGCTTGATCATCATGATCCCCATCATCAGCATGATAGTGATCATCACCAGTTCAGTAATCATGATCAAGTTTTTCCTTATAGACCATCAATGCCTGAACAGTCCAACAACCAATCAGAGGATTTCTTCGCTGATTTAGGAGAAATTGAAGCTGACCCACTAAACCTCTTGTTCTCACAGTGTTTCACAGCAGATGAACAGCAAAAGGGTGGAAACAAGGCTGCCTTGGATCCATTCAATCTCTTTGATTGGTCTGGAGATCAAAACACCAACACCCCCAACAACAATCCTTCATTTGGTCATGAAGCCAAGAGGCATTTATAA

Protein Analysis

496

Amino Acids

53.63

Weight (kDa)

5.95

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 250 - 307 4.9e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1489
AccB1I GGYRCC 1 cut(s) 980
AccII CGCG 1 cut(s) 246
AciI CCGC 4 cut(s) 285, 462, 473, 491
AclWI GGATC 8 cut(s) 91, 218, 242, 869, 954, 1178, 1391, 1404
AcoI YGGCCR 2 cut(s) 183, 923
AcsI RAATTY 3 cut(s) 121, 167, 304
AcuI CTGAAG 1 cut(s) 422
AfaI GTAC 4 cut(s) 239, 372, 770, 1089
AfiI CCNNNNNNNGG 7 cut(s) 132, 512, 621, 788, 1039, 1285, 1463
AflIII ACRYGT 1 cut(s) 882
AgsI TTSAA 4 cut(s) 990, 1125, 1316, 1405
AjiI CACGTC 1 cut(s) 469
AjnI CCWGG 4 cut(s) 373, 544, 672, 920
AjuI GAANNNNNNNTTGG 2 cut(s) 1148, 1180
AloI GAACNNNNNNTCC 2 cut(s) 723, 755
Alw21I GWGCWC 2 cut(s) 367, 912
Alw26I GTCTC 1 cut(s) 1098
AlwI GGATC 8 cut(s) 91, 218, 242, 869, 954, 1178, 1391, 1404
Ama87I CYCGRG 2 cut(s) 449, 605
AoxI GGCC 5 cut(s) 183, 407, 542, 722, 923
ApoI RAATTY 3 cut(s) 121, 167, 304
AseI ATTAAT 1 cut(s) 576
Asp700I GAANNNNTTC 2 cut(s) 738, 1120
AspLEI GCGC 2 cut(s) 355, 703
AspS9I GGNCC 1 cut(s) 542
AsuC2I CCSGG 3 cut(s) 450, 451, 651
AsuHPI GGTGA 7 cut(s) 371, 404, 495, 786, 886, 1007, 1205
AvaI CYCGRG 2 cut(s) 449, 605
BalI TGGCCA 2 cut(s) 185, 925
BamHI GGATCC 1 cut(s) 1396
BanI GGYRCC 1 cut(s) 980
BauI CACGAG 2 cut(s) 260, 345
BbsI GAAGAC 1 cut(s) 207
Bbv12I GWGCWC 2 cut(s) 367, 912
BccI CCATC 8 cut(s) 22, 390, 751, 791, 924, 976, 1197, 1261
BceAI ACGGC 1 cut(s) 340
BciT130I CCWGG 4 cut(s) 375, 546, 674, 922
BclI TGATCA 3 cut(s) 1174, 1207, 1231
BcnI CCSGG 3 cut(s) 450, 451, 651
BcoDI GTCTC 1 cut(s) 1098
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 3 cut(s) 78, 814, 1056
BfoI RGCGCY 1 cut(s) 704
BglII AGATCT 2 cut(s) 441, 630
BlpI GCTNAGC 1 cut(s) 841
Bme1390I CCNGG 7 cut(s) 375, 450, 451, 546, 651, 674, 922
BmeT110I CYCGRG 2 cut(s) 449, 605
BmgBI CACGTC 1 cut(s) 469
BmgT120I GGNCC 1 cut(s) 542
BmiI GGNNCC 2 cut(s) 982, 1398
BmrFI CCNGG 7 cut(s) 375, 450, 451, 546, 651, 674, 922
BmsI GCATC 1 cut(s) 809
BpiI GAAGAC 1 cut(s) 207
BplI GAGNNNNNCTC 2 cut(s) 925, 957
BpmI CTGGAG 4 cut(s) 297, 750, 1080, 1443
Bpu1102I GCTNAGC 1 cut(s) 841
BpuMI CCSGG 3 cut(s) 450, 451, 651
BsaBI GATNNNNATC 2 cut(s) 1188, 1206
BsaJI CCNNGG 7 cut(s) 186, 448, 449, 506, 641, 920, 1392
BsaWI WCCGGW 1 cut(s) 614
BsaXI ACNNNNNCTCC 4 cut(s) 609, 639, 723, 753
Bsc4I CCNNNNNNNGG 7 cut(s) 132, 512, 621, 788, 1039, 1285, 1463
Bse118I RCCGGY 4 cut(s) 281, 947, 977, 983
Bse1I ACTGG 3 cut(s) 733, 1097, 1216
Bse8I GATNNNNATC 2 cut(s) 1188, 1206
BseBI CCWGG 4 cut(s) 375, 546, 674, 922
BseDI CCNNGG 7 cut(s) 186, 448, 449, 506, 641, 920, 1392
BseGI GGATG 5 cut(s) 33, 86, 143, 275, 916
BseJI GATNNNNATC 2 cut(s) 1188, 1206
BseLI CCNNNNNNNGG 7 cut(s) 132, 512, 621, 788, 1039, 1285, 1463
BseMII CTCAG 1 cut(s) 855
BseNI ACTGG 3 cut(s) 733, 1097, 1216
BseRI GAGGAG 4 cut(s) 531, 534, 537, 540
BseYI CCCAGC 3 cut(s) 539, 963, 1033
BsgI GTGCAG 2 cut(s) 25, 647
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 5 cut(s) 185, 409, 544, 724, 925
BshNI GGYRCC 1 cut(s) 980
BsiHKAI GWGCWC 2 cut(s) 367, 912
BsiHKCI CYCGRG 2 cut(s) 449, 605
BsiSI CCGG 8 cut(s) 282, 450, 615, 650, 868, 948, 978, 984
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 7 cut(s) 132, 512, 621, 788, 1039, 1285, 1463
BsmAI GTCTC 1 cut(s) 1098
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 5 cut(s) 185, 409, 544, 724, 925
BsoBI CYCGRG 2 cut(s) 449, 605
Bsp1286I GDGCHC 2 cut(s) 367, 912
Bsp1720I GCTNAGC 1 cut(s) 841
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 4 cut(s) 285, 462, 473, 491
BspANI GGCC 5 cut(s) 185, 409, 544, 724, 925
BspCNI CTCAG 1 cut(s) 854
BspFNI CGCG 1 cut(s) 246
BspHI TCATGA 3 cut(s) 1180, 1228, 1468
BspLI GGNNCC 2 cut(s) 982, 1398
BspMAI CTGCAG 1 cut(s) 1060
BspPI GGATC 8 cut(s) 91, 218, 242, 869, 954, 1178, 1391, 1404
BspQI GCTCTTC 1 cut(s) 662
BspT107I GGYRCC 1 cut(s) 980
BsrFI RCCGGY 4 cut(s) 281, 947, 977, 983
BsrI ACTGG 3 cut(s) 733, 1097, 1216
BssAI RCCGGY 4 cut(s) 281, 947, 977, 983
BssECI CCNNGG 7 cut(s) 186, 448, 449, 506, 641, 920, 1392
BssSI CACGAG 2 cut(s) 260, 345
BssT1I CCWWGG 2 cut(s) 186, 1392
Bst2BI CACGAG 2 cut(s) 260, 345
Bst2UI CCWGG 4 cut(s) 375, 546, 674, 922
Bst4CI ACNGT 7 cut(s) 719, 773, 1092, 1111, 1129, 1269, 1347
Bst6I CTCTTC 2 cut(s) 662, 1131
BstC8I GCNNGC 4 cut(s) 134, 602, 705, 949
BstDEI CTNAG 2 cut(s) 402, 841
BstDSI CCRYGG 2 cut(s) 186, 641
BstEII GGTNACC 2 cut(s) 377, 501
BstF5I GGATG 5 cut(s) 33, 86, 143, 275, 916
BstFNI CGCG 1 cut(s) 246
BstH2I RGCGCY 1 cut(s) 704
BstHHI GCGC 2 cut(s) 355, 703
BstMAI GTCTC 1 cut(s) 1098
BstMWI GCNNNNNNNGC 5 cut(s) 74, 294, 350, 707, 1168
BstNI CCWGG 4 cut(s) 375, 546, 674, 922
BstNSI RCATGY 1 cut(s) 886
BstPI GGTNACC 2 cut(s) 377, 501
BstSCI CCNGG 7 cut(s) 373, 448, 449, 544, 649, 672, 920
BstSFI CTRYAG 3 cut(s) 78, 814, 1056
BstUI CGCG 1 cut(s) 246
BstV2I GAAGAC 1 cut(s) 207
BstX2I RGATCY 3 cut(s) 441, 630, 1396
BstYI RGATCY 3 cut(s) 441, 630, 1396
BsuRI GGCC 5 cut(s) 185, 409, 544, 724, 925
BtgI CCRYGG 2 cut(s) 186, 641
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 5 cut(s) 33, 86, 143, 275, 916
BtsIMutI CAGTG 2 cut(s) 334, 1352
Cac8I GCNNGC 4 cut(s) 134, 602, 705, 949
CciI TCATGA 3 cut(s) 1180, 1228, 1468
CfoI GCGC 2 cut(s) 355, 703
Cfr10I RCCGGY 4 cut(s) 281, 947, 977, 983
Cfr13I GGNCC 1 cut(s) 542
Cfr9I CCCGGG 1 cut(s) 449
CseI GACGC 1 cut(s) 212
CsiI ACCWGGT 1 cut(s) 373
Csp6I GTAC 4 cut(s) 238, 371, 769, 1088
CviQI GTAC 4 cut(s) 238, 371, 769, 1088
DdeI CTNAG 2 cut(s) 402, 841
EaeI YGGCCR 2 cut(s) 183, 923
Eam1104I CTCTTC 2 cut(s) 662, 1131
EarI CTCTTC 2 cut(s) 662, 1131
Eco130I CCWWGG 2 cut(s) 186, 1392
Eco147I AGGCCT 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 422
Eco88I CYCGRG 2 cut(s) 449, 605
Eco91I GGTNACC 2 cut(s) 377, 501
EcoO65I GGTNACC 2 cut(s) 377, 501
EcoRI GAATTC 1 cut(s) 121
EcoRII CCWGG 4 cut(s) 373, 544, 672, 920
EcoT14I CCWWGG 2 cut(s) 186, 1392
ErhI CCWWGG 2 cut(s) 186, 1392
FalI AAGNNNNNCTT 2 cut(s) 1376, 1408
FaqI GGGAC 1 cut(s) 224
FauI CCCGC 1 cut(s) 466
FbaI TGATCA 3 cut(s) 1174, 1207, 1231
FokI GGATG 5 cut(s) 40, 73, 130, 262, 903
FspBI CTAG 1 cut(s) 458
GlaI GCGC 2 cut(s) 354, 702
GsaI CCCAGC 3 cut(s) 543, 967, 1037
GsuI CTGGAG 4 cut(s) 297, 750, 1080, 1443
HaeII RGCGCY 1 cut(s) 704
HaeIII GGCC 5 cut(s) 185, 409, 544, 724, 925
HapII CCGG 8 cut(s) 282, 450, 615, 650, 868, 948, 978, 984
HgaI GACGC 1 cut(s) 212
HhaI GCGC 2 cut(s) 355, 703
Hin6I GCGC 2 cut(s) 353, 701
HinP1I GCGC 2 cut(s) 353, 701
HinfI GANTC 4 cut(s) 310, 482, 531, 646
HpaII CCGG 8 cut(s) 282, 450, 615, 650, 868, 948, 978, 984
HphI GGTGA 7 cut(s) 371, 404, 495, 786, 886, 1007, 1205
Hpy166II GTNNAC 2 cut(s) 273, 794
Hpy188I TCNGA 8 cut(s) 112, 127, 148, 441, 748, 777, 907, 1285
Hpy188III TCNNGA 8 cut(s) 172, 260, 314, 607, 1181, 1229, 1422, 1469
Hpy8I GTNNAC 2 cut(s) 273, 794
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 3 cut(s) 657, 676, 1467
HpyCH4III ACNGT 7 cut(s) 719, 773, 1092, 1111, 1129, 1269, 1347
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 8 cut(s) 6, 414, 581, 628, 822, 1058, 1106, 1162
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 294, 350, 707, 1168
HpyF3I CTNAG 2 cut(s) 402, 841
HpySE526I ACGT 1 cut(s) 468
HspAI GCGC 2 cut(s) 353, 701
KroI GCCGGC 1 cut(s) 947
KroNI GCCGGC 1 cut(s) 949
Ksp22I TGATCA 3 cut(s) 1174, 1207, 1231
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 1 cut(s) 1168
LweI GCATC 1 cut(s) 809
MabI ACCWGGT 1 cut(s) 373
MaeI CTAG 1 cut(s) 458
MaeII ACGT 1 cut(s) 468
MaeIII GTNAC 4 cut(s) 377, 397, 501, 809
MboII GAAGA 8 cut(s) 203, 212, 248, 679, 691, 1055, 1148, 1285
MfeI CAATTG 2 cut(s) 21, 1163
MflI RGATCY 3 cut(s) 441, 630, 1396
MhlI GDGCHC 2 cut(s) 367, 912
MlsI TGGCCA 2 cut(s) 185, 925
MluCI AATT 8 cut(s) 21, 34, 121, 167, 214, 304, 1163, 1311
MluNI TGGCCA 2 cut(s) 185, 925
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 4 cut(s) 150, 541, 1093, 1296
Mox20I TGGCCA 2 cut(s) 185, 925
MroNI GCCGGC 1 cut(s) 947
MroXI GAANNNNTTC 2 cut(s) 738, 1120
MscI TGGCCA 2 cut(s) 185, 925
MseI TTAA 4 cut(s) 50, 576, 1101, 1131
MslI CAYNNNNRTG 3 cut(s) 390, 1197, 1203
Msp20I TGGCCA 2 cut(s) 185, 925
MspA1I CMGCKG 2 cut(s) 132, 1061
MspI CCGG 8 cut(s) 282, 450, 615, 650, 868, 948, 978, 984
MspR9I CCNGG 7 cut(s) 375, 450, 451, 546, 651, 674, 922
MunI CAATTG 2 cut(s) 21, 1163
MvaI CCWGG 4 cut(s) 375, 546, 674, 922
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 5 cut(s) 74, 294, 350, 707, 1168
NaeI GCCGGC 1 cut(s) 949
NciI CCSGG 3 cut(s) 450, 451, 651
NcoI CCATGG 1 cut(s) 186
NgoMIV GCCGGC 1 cut(s) 947
NlaIV GGNNCC 2 cut(s) 982, 1398
NmuCI GTSAC 2 cut(s) 377, 501
NspI RCATGY 1 cut(s) 886
PaeR7I CTCGAG 1 cut(s) 605
PagI TCATGA 3 cut(s) 1180, 1228, 1468
PceI AGGCCT 1 cut(s) 409
PciI ACATGT 1 cut(s) 882
PciSI GCTCTTC 1 cut(s) 662
PdiI GCCGGC 1 cut(s) 949
PdmI GAANNNNTTC 2 cut(s) 738, 1120
PfeI GAWTC 3 cut(s) 482, 531, 646
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PscI ACATGT 1 cut(s) 882
PshBI ATTAAT 1 cut(s) 576
PsiI TTATAA 1 cut(s) 1489
Psp6I CCWGG 4 cut(s) 373, 544, 672, 920
PspEI GGTNACC 2 cut(s) 377, 501
PspFI CCCAGC 3 cut(s) 539, 963, 1033
PspGI CCWGG 4 cut(s) 373, 544, 672, 920
PspN4I GGNNCC 2 cut(s) 982, 1398
PspPI GGNCC 1 cut(s) 542
PstI CTGCAG 1 cut(s) 1060
PsuI RGATCY 3 cut(s) 441, 630, 1396
PvuII CAGCTG 2 cut(s) 132, 1061
RsaI GTAC 4 cut(s) 239, 372, 770, 1089
RsaNI GTAC 4 cut(s) 238, 371, 769, 1088
RseI CAYNNNNRTG 3 cut(s) 390, 1197, 1203
SapI GCTCTTC 1 cut(s) 662
SaqAI TTAA 4 cut(s) 50, 576, 1101, 1131
Sau96I GGNCC 1 cut(s) 542
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 7 cut(s) 375, 450, 451, 546, 651, 674, 922
SduI GDGCHC 2 cut(s) 367, 912
SexAI ACCWGGT 1 cut(s) 373
SfaNI GCATC 1 cut(s) 809
SfcI CTRYAG 3 cut(s) 78, 814, 1056
Sfr274I CTCGAG 1 cut(s) 605
SgrAI CRCCGGYG 1 cut(s) 281
SlaI CTCGAG 1 cut(s) 605
SmaI CCCGGG 1 cut(s) 451
SmiMI CAYNNNNRTG 3 cut(s) 390, 1197, 1203
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
Sse9I AATT 8 cut(s) 21, 34, 121, 167, 214, 304, 1163, 1311
SseBI AGGCCT 1 cut(s) 409
SsiI CCGC 4 cut(s) 285, 462, 473, 491
SspMI CTAG 1 cut(s) 458
StuI AGGCCT 1 cut(s) 409
StyD4I CCNGG 7 cut(s) 373, 448, 449, 544, 649, 672, 920
StyI CCWWGG 2 cut(s) 186, 1392
TaaI ACNGT 7 cut(s) 719, 773, 1092, 1111, 1129, 1269, 1347
TaiI ACGT 1 cut(s) 471
TaqI TCGA 4 cut(s) 64, 173, 308, 606
TasI AATT 8 cut(s) 21, 34, 121, 167, 214, 304, 1163, 1311
TatI WGTACW 1 cut(s) 1087
TauI GCSGC 2 cut(s) 288, 465
TfiI GAWTC 3 cut(s) 482, 531, 646
Tru1I TTAA 4 cut(s) 50, 576, 1101, 1131
Tru9I TTAA 4 cut(s) 50, 576, 1101, 1131
TscAI CASTG 2 cut(s) 334, 1352
TseFI GTSAC 2 cut(s) 377, 501
Tsp45I GTSAC 2 cut(s) 377, 501
TspDTI ATGAA 9 cut(s) 132, 134, 179, 222, 335, 1085, 1377, 1449, 1485
TspGWI ACGGA 2 cut(s) 216, 658
TspMI CCCGGG 1 cut(s) 449
TspRI CASTG 2 cut(s) 334, 1352
VspI ATTAAT 1 cut(s) 576
XapI RAATTY 3 cut(s) 121, 167, 304
XceI RCATGY 1 cut(s) 886
XcmI CCANNNNNNNNNTGG 1 cut(s) 750
XhoI CTCGAG 1 cut(s) 605
XmaI CCCGGG 1 cut(s) 449
XmnI GAANNNNTTC 2 cut(s) 738, 1120
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.