Rh5DG133000
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010362
Physical Location & Seq
Forward (+)
13157302 .. 13161068
3767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG133000.1

Sequence Viewer

Length: 1503 bp
ATGTGCAGCTTGTCTATGCCAATTGGGATGGAGAATTATCAAGGCGATTTAACTGACATACTTCGAGCTGGTGCTGCTGCTGCTGCTACAGGATCATCCTCATCATCGGCTTATCATTCCGAAACAATGAATTCCGACAGCTGGCAGTTTTCATCCGATGATAACTATTCTATGAAATTCTCGACATCAATGGCCATGGAAGAAGTAGAAGACGCAAGGGACAATTTCGGCGATCCGTTTTCATCGTACATACGCGATCCACTTCTTCACGAGCTGTCGTCCACTTCCACCGGCGGCTTTTTCAGCAGCCCAAATTCGACTCCAGAAATGAACATCAGTGTAGATATGGAAGCTCGTGCTGCGCCGTTTGGTGCTCATGTACCTGGTCACCATCATCAAGGTGAGGTTACTAAGAGGCCTTGCAACAACATATTCTCTCGTATGCTTCAGATCTCCCCCGGGAAGCTAGGCGGCGACGTGGCTGGGTGTGATTCGGTGGCGGTGTTGGGGTCACCGAGGGGGGGAGGAGGAGGAGGAGGAGGAATCAAGGCTGGGCCTGGTGGTGTTATGGTTGGAAGCGACATGATTAATGCAAACAGCTCAAAAGGTTGCTTGCTCGAGAACACCGGAGTTGGGGTGCAGATCTCATCTCCACGGAATCCGGGTATCAAGCGAAGGAAGAGCCAGGCGAAGAAGGTGGTATGTATTCCAGCGCCAGCAGCAGCAAACAGTAGGCCAACTGGAGAAGTAGTTCCATCTGATCTGTGGGCATGGAGAAAGTACGGTCAGAAACCCATCAAAGGTTCACCTTATCCAAGAGGTTACTATAGATGCAGCAGCTCAAAGGGTTGCTCAGCAAGGAAACAAGTAGAGCGTAGCCGGACTGATCCAAACATGTTGGTCATCACCTACACTTCCGAGCACAACCATCCCTGGCCAACCCAGAGAAATGCTCTTGCAGGCTCAACCCGATCCCAGCCATCAAAAGCCGGTGCCGGTTCAAAGATCTCTCCTAATACTGCTCATCAGCAAAAGCCAGATAGCCCAGCAAAGGAAGAACAAAAGGCTGCAGCTGACAGCATGAACGAGAATAATCATGTACTGTCTCCAGTAATTGCAACTGTTGGAAGTAGTTCAACAGTTAAAGAAGAGTTTGAGGACATTGAGAAGCTGCAATTGGAGCTTGATCATCATGATCCCCATCATCAGCATGATAGTGATCATCACCAGTTCAGTAATCATGATCAAGGTTTTCCTTATAGACCATCAATGCCTGAACAGTCCAACAACCAATCAGAGGATTTCTTCGCTGATTTAGGAGAAATTGAAGCTGACCCACTAAACCTCTTGTTCTCACAGTGTTTCACAGCAGATGAACAGCAAAAGGGTGGAAACAAGGCTGCCTTGGATCCATTCAATCTCTTTGATTGGTCTGGAGATCAAAACACCAACACCCCCAACAACAATCCTTCATTTGGTCATGAAGCCAAGAGGCGTTTATGA

Protein Analysis

500

Amino Acids

53.88

Weight (kDa)

5.95

Isoelectric Point (pI)

51.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 254 - 311 4.9e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 992
AccII CGCG 1 cut(s) 255
AciI CCGC 3 cut(s) 294, 471, 500
AclWI GGATC 8 cut(s) 100, 227, 251, 881, 966, 1190, 1403, 1416
AcoI YGGCCR 2 cut(s) 192, 935
AcsI RAATTY 3 cut(s) 130, 176, 313
AcuI CTGAAG 1 cut(s) 431
AfaI GTAC 4 cut(s) 248, 381, 782, 1101
AfiI CCNNNNNNNGG 7 cut(s) 141, 521, 633, 800, 1051, 1297, 1475
AflIII ACRYGT 1 cut(s) 894
AgsI TTSAA 4 cut(s) 1002, 1137, 1328, 1417
AjiI CACGTC 1 cut(s) 478
AjnI CCWGG 4 cut(s) 382, 556, 684, 932
AjuI GAANNNNNNNTTGG 2 cut(s) 1160, 1192
AloI GAACNNNNNNTCC 2 cut(s) 735, 767
Alw21I GWGCWC 2 cut(s) 376, 924
Alw26I GTCTC 1 cut(s) 1110
AlwI GGATC 8 cut(s) 100, 227, 251, 881, 966, 1190, 1403, 1416
Ama87I CYCGRG 2 cut(s) 458, 617
AoxI GGCC 5 cut(s) 192, 416, 554, 734, 935
ApoI RAATTY 3 cut(s) 130, 176, 313
AseI ATTAAT 1 cut(s) 588
Asp700I GAANNNNTTC 2 cut(s) 750, 1132
AspLEI GCGC 2 cut(s) 364, 715
AspS9I GGNCC 1 cut(s) 554
AsuC2I CCSGG 3 cut(s) 459, 460, 663
AsuHPI GGTGA 6 cut(s) 380, 413, 504, 798, 898, 1217
AvaI CYCGRG 2 cut(s) 458, 617
BalI TGGCCA 2 cut(s) 194, 937
BamHI GGATCC 1 cut(s) 1408
BanI GGYRCC 1 cut(s) 992
BauI CACGAG 2 cut(s) 269, 354
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 2 cut(s) 376, 924
BccI CCATC 8 cut(s) 22, 399, 763, 803, 936, 988, 1209, 1273
BceAI ACGGC 1 cut(s) 349
BciT130I CCWGG 4 cut(s) 384, 558, 686, 934
BclI TGATCA 3 cut(s) 1186, 1219, 1243
BcnI CCSGG 3 cut(s) 459, 460, 663
BcoDI GTCTC 1 cut(s) 1110
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 3 cut(s) 87, 826, 1068
BfoI RGCGCY 1 cut(s) 716
BglII AGATCT 3 cut(s) 450, 642, 1005
BlpI GCTNAGC 1 cut(s) 853
Bme1390I CCNGG 7 cut(s) 384, 459, 460, 558, 663, 686, 934
BmeT110I CYCGRG 2 cut(s) 458, 617
BmgBI CACGTC 1 cut(s) 478
BmgT120I GGNCC 1 cut(s) 554
BmiI GGNNCC 2 cut(s) 994, 1410
BmrFI CCNGG 7 cut(s) 384, 459, 460, 558, 663, 686, 934
BmsI GCATC 1 cut(s) 821
BpiI GAAGAC 1 cut(s) 216
BplI GAGNNNNNCTC 2 cut(s) 937, 969
BpmI CTGGAG 4 cut(s) 306, 762, 1092, 1455
Bpu1102I GCTNAGC 1 cut(s) 853
BpuMI CCSGG 3 cut(s) 459, 460, 663
BsaBI GATNNNNATC 2 cut(s) 1200, 1218
BsaJI CCNNGG 7 cut(s) 195, 457, 458, 515, 653, 932, 1404
BsaWI WCCGGW 1 cut(s) 626
BsaXI ACNNNNNCTCC 4 cut(s) 621, 651, 735, 765
Bsc4I CCNNNNNNNGG 7 cut(s) 141, 521, 633, 800, 1051, 1297, 1475
Bse118I RCCGGY 3 cut(s) 290, 989, 995
Bse1I ACTGG 3 cut(s) 745, 1109, 1228
Bse8I GATNNNNATC 2 cut(s) 1200, 1218
BseBI CCWGG 4 cut(s) 384, 558, 686, 934
BseDI CCNNGG 7 cut(s) 195, 457, 458, 515, 653, 932, 1404
BseGI GGATG 4 cut(s) 33, 95, 152, 928
BseJI GATNNNNATC 2 cut(s) 1200, 1218
BseLI CCNNNNNNNGG 7 cut(s) 141, 521, 633, 800, 1051, 1297, 1475
BseMII CTCAG 1 cut(s) 867
BseNI ACTGG 3 cut(s) 745, 1109, 1228
BseRI GAGGAG 5 cut(s) 540, 543, 546, 549, 552
BseYI CCCAGC 4 cut(s) 482, 551, 975, 1045
BsgI GTGCAG 2 cut(s) 25, 659
Bsh1236I CGCG 1 cut(s) 255
BshFI GGCC 5 cut(s) 194, 418, 556, 736, 937
BshNI GGYRCC 1 cut(s) 992
BsiHKAI GWGCWC 2 cut(s) 376, 924
BsiHKCI CYCGRG 2 cut(s) 458, 617
BsiSI CCGG 7 cut(s) 291, 459, 627, 662, 880, 990, 996
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 7 cut(s) 141, 521, 633, 800, 1051, 1297, 1475
BsmAI GTCTC 1 cut(s) 1110
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 5 cut(s) 194, 418, 556, 736, 937
BsoBI CYCGRG 2 cut(s) 458, 617
Bsp1286I GDGCHC 2 cut(s) 376, 924
Bsp1720I GCTNAGC 1 cut(s) 853
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 3 cut(s) 294, 471, 500
BspANI GGCC 5 cut(s) 194, 418, 556, 736, 937
BspCNI CTCAG 1 cut(s) 866
BspFNI CGCG 1 cut(s) 255
BspHI TCATGA 3 cut(s) 1192, 1240, 1480
BspLI GGNNCC 2 cut(s) 994, 1410
BspMAI CTGCAG 1 cut(s) 1072
BspPI GGATC 8 cut(s) 100, 227, 251, 881, 966, 1190, 1403, 1416
BspQI GCTCTTC 1 cut(s) 674
BspT107I GGYRCC 1 cut(s) 992
BsrFI RCCGGY 3 cut(s) 290, 989, 995
BsrI ACTGG 3 cut(s) 745, 1109, 1228
BssAI RCCGGY 3 cut(s) 290, 989, 995
BssECI CCNNGG 7 cut(s) 195, 457, 458, 515, 653, 932, 1404
BssSI CACGAG 2 cut(s) 269, 354
BssT1I CCWWGG 2 cut(s) 195, 1404
Bst2BI CACGAG 2 cut(s) 269, 354
Bst2UI CCWGG 4 cut(s) 384, 558, 686, 934
Bst4CI ACNGT 7 cut(s) 731, 785, 1104, 1123, 1141, 1281, 1359
Bst6I CTCTTC 2 cut(s) 674, 1143
BstC8I GCNNGC 4 cut(s) 143, 614, 717, 961
BstDEI CTNAG 2 cut(s) 411, 853
BstDSI CCRYGG 2 cut(s) 195, 653
BstEII GGTNACC 2 cut(s) 386, 510
BstF5I GGATG 4 cut(s) 33, 95, 152, 928
BstFNI CGCG 1 cut(s) 255
BstH2I RGCGCY 1 cut(s) 716
BstHHI GCGC 2 cut(s) 364, 715
BstMAI GTCTC 1 cut(s) 1110
BstMWI GCNNNNNNNGC 7 cut(s) 74, 80, 83, 303, 359, 719, 1180
BstNI CCWGG 4 cut(s) 384, 558, 686, 934
BstNSI RCATGY 1 cut(s) 898
BstPI GGTNACC 2 cut(s) 386, 510
BstSCI CCNGG 7 cut(s) 382, 457, 458, 556, 661, 684, 932
BstSFI CTRYAG 3 cut(s) 87, 826, 1068
BstUI CGCG 1 cut(s) 255
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 4 cut(s) 450, 642, 1005, 1408
BstYI RGATCY 4 cut(s) 450, 642, 1005, 1408
BsuRI GGCC 5 cut(s) 194, 418, 556, 736, 937
BtgI CCRYGG 2 cut(s) 195, 653
BtrI CACGTC 1 cut(s) 478
BtsCI GGATG 4 cut(s) 33, 95, 152, 928
BtsIMutI CAGTG 2 cut(s) 343, 1364
Cac8I GCNNGC 4 cut(s) 143, 614, 717, 961
CciI TCATGA 3 cut(s) 1192, 1240, 1480
CfoI GCGC 2 cut(s) 364, 715
Cfr10I RCCGGY 3 cut(s) 290, 989, 995
Cfr13I GGNCC 1 cut(s) 554
Cfr9I CCCGGG 1 cut(s) 458
CseI GACGC 1 cut(s) 221
CsiI ACCWGGT 1 cut(s) 382
Csp6I GTAC 4 cut(s) 247, 380, 781, 1100
CviQI GTAC 4 cut(s) 247, 380, 781, 1100
DdeI CTNAG 2 cut(s) 411, 853
EaeI YGGCCR 2 cut(s) 192, 935
Eam1104I CTCTTC 2 cut(s) 674, 1143
EarI CTCTTC 2 cut(s) 674, 1143
Eco130I CCWWGG 2 cut(s) 195, 1404
Eco147I AGGCCT 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 431
Eco88I CYCGRG 2 cut(s) 458, 617
Eco91I GGTNACC 2 cut(s) 386, 510
EcoO65I GGTNACC 2 cut(s) 386, 510
EcoRI GAATTC 1 cut(s) 130
EcoRII CCWGG 4 cut(s) 382, 556, 684, 932
EcoT14I CCWWGG 2 cut(s) 195, 1404
ErhI CCWWGG 2 cut(s) 195, 1404
FalI AAGNNNNNCTT 2 cut(s) 1388, 1420
FaqI GGGAC 1 cut(s) 233
FbaI TGATCA 3 cut(s) 1186, 1219, 1243
FokI GGATG 4 cut(s) 40, 82, 139, 915
FspBI CTAG 1 cut(s) 467
GlaI GCGC 2 cut(s) 363, 714
GsaI CCCAGC 4 cut(s) 486, 555, 979, 1049
GsuI CTGGAG 4 cut(s) 306, 762, 1092, 1455
HaeII RGCGCY 1 cut(s) 716
HaeIII GGCC 5 cut(s) 194, 418, 556, 736, 937
HapII CCGG 7 cut(s) 291, 459, 627, 662, 880, 990, 996
HgaI GACGC 1 cut(s) 221
HhaI GCGC 2 cut(s) 364, 715
Hin6I GCGC 2 cut(s) 362, 713
HinP1I GCGC 2 cut(s) 362, 713
HinfI GANTC 4 cut(s) 319, 491, 543, 658
HpaII CCGG 7 cut(s) 291, 459, 627, 662, 880, 990, 996
HphI GGTGA 6 cut(s) 380, 413, 504, 798, 898, 1217
Hpy166II GTNNAC 2 cut(s) 282, 806
Hpy188I TCNGA 8 cut(s) 121, 136, 157, 450, 760, 789, 919, 1297
Hpy188III TCNNGA 8 cut(s) 181, 269, 323, 619, 1193, 1241, 1434, 1481
Hpy8I GTNNAC 2 cut(s) 282, 806
Hpy99I CGWCG 1 cut(s) 479
HpyAV CCTTC 3 cut(s) 669, 688, 1479
HpyCH4III ACNGT 7 cut(s) 731, 785, 1104, 1123, 1141, 1281, 1359
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 9 cut(s) 6, 423, 593, 640, 834, 959, 1070, 1118, 1174
HpyF10VI GCNNNNNNNGC 7 cut(s) 74, 80, 83, 303, 359, 719, 1180
HpyF3I CTNAG 2 cut(s) 411, 853
HpySE526I ACGT 1 cut(s) 477
HspAI GCGC 2 cut(s) 362, 713
Ksp22I TGATCA 3 cut(s) 1186, 1219, 1243
LguI GCTCTTC 1 cut(s) 674
LmnI GCTCC 1 cut(s) 1180
LweI GCATC 1 cut(s) 821
MabI ACCWGGT 1 cut(s) 382
MaeI CTAG 1 cut(s) 467
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 4 cut(s) 386, 406, 510, 821
MboII GAAGA 8 cut(s) 212, 221, 257, 691, 703, 1067, 1160, 1297
MfeI CAATTG 2 cut(s) 21, 1175
MflI RGATCY 4 cut(s) 450, 642, 1005, 1408
MhlI GDGCHC 2 cut(s) 376, 924
MlsI TGGCCA 2 cut(s) 194, 937
MluCI AATT 9 cut(s) 21, 34, 130, 176, 223, 313, 1113, 1175, 1323
MluNI TGGCCA 2 cut(s) 194, 937
MlyI GAGTC 1 cut(s) 313
MmeI TCCRAC 4 cut(s) 159, 553, 1105, 1308
Mox20I TGGCCA 2 cut(s) 194, 937
MroXI GAANNNNTTC 2 cut(s) 750, 1132
MscI TGGCCA 2 cut(s) 194, 937
MseI TTAA 3 cut(s) 50, 588, 1143
MslI CAYNNNNRTG 3 cut(s) 399, 1209, 1215
Msp20I TGGCCA 2 cut(s) 194, 937
MspA1I CMGCKG 2 cut(s) 141, 1073
MspI CCGG 7 cut(s) 291, 459, 627, 662, 880, 990, 996
MspR9I CCNGG 7 cut(s) 384, 459, 460, 558, 663, 686, 934
MunI CAATTG 2 cut(s) 21, 1175
MvaI CCWGG 4 cut(s) 384, 558, 686, 934
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 7 cut(s) 74, 80, 83, 303, 359, 719, 1180
NciI CCSGG 3 cut(s) 459, 460, 663
NcoI CCATGG 1 cut(s) 195
NlaIV GGNNCC 2 cut(s) 994, 1410
NmuCI GTSAC 2 cut(s) 386, 510
NspI RCATGY 1 cut(s) 898
PaeR7I CTCGAG 1 cut(s) 617
PagI TCATGA 3 cut(s) 1192, 1240, 1480
PceI AGGCCT 1 cut(s) 418
PciI ACATGT 1 cut(s) 894
PciSI GCTCTTC 1 cut(s) 674
PdmI GAANNNNTTC 2 cut(s) 750, 1132
PfeI GAWTC 3 cut(s) 491, 543, 658
PleI GAGTC 1 cut(s) 313
PpsI GAGTC 1 cut(s) 313
PscI ACATGT 1 cut(s) 894
PshBI ATTAAT 1 cut(s) 588
Psp6I CCWGG 4 cut(s) 382, 556, 684, 932
PspEI GGTNACC 2 cut(s) 386, 510
PspFI CCCAGC 4 cut(s) 482, 551, 975, 1045
PspGI CCWGG 4 cut(s) 382, 556, 684, 932
PspN4I GGNNCC 2 cut(s) 994, 1410
PspPI GGNCC 1 cut(s) 554
PstI CTGCAG 1 cut(s) 1072
PsuI RGATCY 4 cut(s) 450, 642, 1005, 1408
PvuII CAGCTG 2 cut(s) 141, 1073
RsaI GTAC 4 cut(s) 248, 381, 782, 1101
RsaNI GTAC 4 cut(s) 247, 380, 781, 1100
RseI CAYNNNNRTG 3 cut(s) 399, 1209, 1215
SapI GCTCTTC 1 cut(s) 674
SaqAI TTAA 3 cut(s) 50, 588, 1143
Sau96I GGNCC 1 cut(s) 554
SchI GAGTC 1 cut(s) 313
ScrFI CCNGG 7 cut(s) 384, 459, 460, 558, 663, 686, 934
SduI GDGCHC 2 cut(s) 376, 924
SexAI ACCWGGT 1 cut(s) 382
SfaNI GCATC 1 cut(s) 821
SfcI CTRYAG 3 cut(s) 87, 826, 1068
Sfr274I CTCGAG 1 cut(s) 617
SgrAI CRCCGGYG 1 cut(s) 290
SlaI CTCGAG 1 cut(s) 617
SmaI CCCGGG 1 cut(s) 460
SmiMI CAYNNNNRTG 3 cut(s) 399, 1209, 1215
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
Sse9I AATT 9 cut(s) 21, 34, 130, 176, 223, 313, 1113, 1175, 1323
SseBI AGGCCT 1 cut(s) 418
SsiI CCGC 3 cut(s) 294, 471, 500
SspMI CTAG 1 cut(s) 467
StuI AGGCCT 1 cut(s) 418
StyD4I CCNGG 7 cut(s) 382, 457, 458, 556, 661, 684, 932
StyI CCWWGG 2 cut(s) 195, 1404
TaaI ACNGT 7 cut(s) 731, 785, 1104, 1123, 1141, 1281, 1359
TaiI ACGT 1 cut(s) 480
TaqI TCGA 4 cut(s) 64, 182, 317, 618
TasI AATT 9 cut(s) 21, 34, 130, 176, 223, 313, 1113, 1175, 1323
TatI WGTACW 1 cut(s) 1099
TauI GCSGC 2 cut(s) 297, 474
TfiI GAWTC 3 cut(s) 491, 543, 658
Tru1I TTAA 3 cut(s) 50, 588, 1143
Tru9I TTAA 3 cut(s) 50, 588, 1143
TscAI CASTG 2 cut(s) 343, 1364
TseFI GTSAC 2 cut(s) 386, 510
Tsp45I GTSAC 2 cut(s) 386, 510
TspDTI ATGAA 9 cut(s) 141, 143, 188, 231, 344, 1097, 1389, 1461, 1497
TspGWI ACGGA 2 cut(s) 225, 670
TspMI CCCGGG 1 cut(s) 458
TspRI CASTG 2 cut(s) 343, 1364
VspI ATTAAT 1 cut(s) 588
XapI RAATTY 3 cut(s) 130, 176, 313
XceI RCATGY 1 cut(s) 898
XcmI CCANNNNNNNNNTGG 1 cut(s) 762
XhoI CTCGAG 1 cut(s) 617
XmaI CCCGGG 1 cut(s) 458
XmnI GAANNNNTTC 2 cut(s) 750, 1132
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.