Rh5BG131300
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010362
Physical Location & Seq
Forward (+)
12957864 .. 12958556
693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG131300.1

Sequence Viewer

Length: 693 bp
ATGTGCAGAGGTTACTATAGATGCAGCAGCTCAAAGGGTTGCTCAGCAAGGAAACAAGTAGAGCGTAGCCGGACTGATCCAAACATGTTGGTCATCACCTACACTTCCGAGCACAACCATCCCTGGCCAACCCAGAGAAATGCTCTCGCCGGCTCAACCCGATCCCAGCCATCAAAAGCCGGTGCCGGTTCAAAGACCTCTCCTAATACTGCTCATCACCAAAAGCCAGATAGCCCAGCAAAGGAAGAACAAAAGGCTGCAGCTGACAGCATGAACGAGAATAATCATGTACTGTCTCCAGTTAATGCAACTGTTGGAAGTAGTTCAACAGTTAAAGAAGAGTTTGAGGACATTGAGAAGCTGCAATTGGAGCTTGATCATCATGATCCCCATCATCAGCATGATAGTGATCATCACCAGTTCAGTAATCATGATCAAGGTTTTCCTTACAGACCATCAATGCCTGAACAGTCCAACAACCAATCAGAGGATTTCTTCGCTGATTTAGGAGAAATTGAAGCTGACCCACTAAACCTCTTGTTCTCACAGTGTTTCACATCAGATGAACAGCAAAAGGGGGGAAACAAGGCTGCCTTGGATCCATTCAATCTCTTTGATTGGTCTGGAGATCAAAACACCAACACCCCCAACAACAATCCTTCATTTGGTCATGAAGCCAAGAGGCGTTTATAA

Protein Analysis

230

Amino Acids

25.73

Weight (kDa)

5.73

Isoelectric Point (pI)

54.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 3 - 41 1.5e-13 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 691
AccB1I GGYRCC 1 cut(s) 182
AclWI GGATC 5 cut(s) 71, 156, 380, 593, 606
AcoI YGGCCR 1 cut(s) 125
AfaI GTAC 1 cut(s) 291
AfiI CCNNNNNNNGG 3 cut(s) 241, 487, 665
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 4 cut(s) 192, 327, 518, 607
AjnI CCWGG 1 cut(s) 122
AjuI GAANNNNNNNTTGG 2 cut(s) 350, 382
AluBI AGCT 5 cut(s) 30, 263, 361, 373, 521
AluI AGCT 5 cut(s) 30, 263, 361, 373, 521
Alw21I GWGCWC 1 cut(s) 114
Alw26I GTCTC 1 cut(s) 300
AlwI GGATC 5 cut(s) 71, 156, 380, 593, 606
AoxI GGCC 1 cut(s) 125
ApeKI GCWGC 6 cut(s) 24, 27, 257, 260, 361, 590
Asp700I GAANNNNTTC 1 cut(s) 322
AsuHPI GGTGA 3 cut(s) 88, 209, 407
BalI TGGCCA 1 cut(s) 127
BamHI GGATCC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 114
BbvI GCAGC 6 cut(s) 36, 39, 244, 272, 348, 577
BccI CCATC 4 cut(s) 126, 178, 399, 463
BciT130I CCWGG 1 cut(s) 124
BclI TGATCA 3 cut(s) 376, 409, 433
BcoDI GTCTC 1 cut(s) 300
BfmI CTRYAG 2 cut(s) 16, 258
BisI GCNGC 6 cut(s) 25, 28, 258, 261, 362, 591
BlpI GCTNAGC 1 cut(s) 43
BlsI GCNGC 6 cut(s) 26, 29, 259, 262, 363, 592
Bme1390I CCNGG 1 cut(s) 124
BmiI GGNNCC 2 cut(s) 184, 600
BmrFI CCNGG 1 cut(s) 124
BmsI GCATC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 127, 159
BpmI CTGGAG 2 cut(s) 282, 645
Bpu1102I GCTNAGC 1 cut(s) 43
BsaBI GATNNNNATC 2 cut(s) 390, 408
BsaJI CCNNGG 2 cut(s) 122, 594
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 487, 665
Bse118I RCCGGY 3 cut(s) 149, 179, 185
Bse1I ACTGG 2 cut(s) 299, 418
Bse8I GATNNNNATC 2 cut(s) 390, 408
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 2 cut(s) 122, 594
BseGI GGATG 1 cut(s) 118
BseJI GATNNNNATC 2 cut(s) 390, 408
BseLI CCNNNNNNNGG 3 cut(s) 241, 487, 665
BseMII CTCAG 1 cut(s) 57
BseNI ACTGG 2 cut(s) 299, 418
BseXI GCAGC 6 cut(s) 36, 39, 244, 272, 348, 577
BseYI CCCAGC 2 cut(s) 165, 235
BsgI GTGCAG 1 cut(s) 25
BshFI GGCC 1 cut(s) 127
BshNI GGYRCC 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 114
BsiSI CCGG 4 cut(s) 70, 150, 180, 186
BslI CCNNNNNNNGG 3 cut(s) 241, 487, 665
BsmAI GTCTC 1 cut(s) 300
BsnI GGCC 1 cut(s) 127
Bsp1286I GDGCHC 1 cut(s) 114
Bsp143I GATC 8 cut(s) 76, 161, 376, 385, 409, 433, 598, 628
Bsp1720I GCTNAGC 1 cut(s) 43
BspANI GGCC 1 cut(s) 127
BspCNI CTCAG 1 cut(s) 56
BspHI TCATGA 3 cut(s) 382, 430, 670
BspLI GGNNCC 2 cut(s) 184, 600
BspMAI CTGCAG 1 cut(s) 262
BspPI GGATC 5 cut(s) 71, 156, 380, 593, 606
BspT107I GGYRCC 1 cut(s) 182
BsrFI RCCGGY 3 cut(s) 149, 179, 185
BsrI ACTGG 2 cut(s) 299, 418
BssAI RCCGGY 3 cut(s) 149, 179, 185
BssECI CCNNGG 2 cut(s) 122, 594
BssMI GATC 8 cut(s) 76, 161, 376, 385, 409, 433, 598, 628
BssT1I CCWWGG 1 cut(s) 594
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 5 cut(s) 294, 313, 331, 471, 549
Bst6I CTCTTC 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 151
BstDEI CTNAG 1 cut(s) 43
BstF5I GGATG 1 cut(s) 118
BstKTI GATC 8 cut(s) 79, 164, 379, 388, 412, 436, 601, 631
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 8 cut(s) 76, 161, 376, 385, 409, 433, 598, 628
BstMWI GCNNNNNNNGC 1 cut(s) 370
BstNI CCWGG 1 cut(s) 124
BstNSI RCATGY 1 cut(s) 88
BstSCI CCNGG 1 cut(s) 122
BstSFI CTRYAG 2 cut(s) 16, 258
BstV1I GCAGC 6 cut(s) 36, 39, 244, 272, 348, 577
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 1 cut(s) 127
BtsCI GGATG 1 cut(s) 118
BtsIMutI CAGTG 1 cut(s) 554
Cac8I GCNNGC 1 cut(s) 151
CciI TCATGA 3 cut(s) 382, 430, 670
Cfr10I RCCGGY 3 cut(s) 149, 179, 185
Csp6I GTAC 1 cut(s) 290
CviAII CATG 7 cut(s) 85, 271, 287, 383, 401, 431, 671
CviQI GTAC 1 cut(s) 290
DdeI CTNAG 1 cut(s) 43
DpnI GATC 8 cut(s) 78, 163, 378, 387, 411, 435, 600, 630
DpnII GATC 8 cut(s) 76, 161, 376, 385, 409, 433, 598, 628
EaeI YGGCCR 1 cut(s) 125
Eam1104I CTCTTC 1 cut(s) 333
EarI CTCTTC 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 594
EcoRII CCWGG 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 594
ErhI CCWWGG 1 cut(s) 594
FaeI CATG 7 cut(s) 88, 274, 290, 386, 404, 434, 674
FaiI YATR 9 cut(s) 18, 86, 272, 288, 384, 402, 432, 672, 691
FalI AAGNNNNNCTT 2 cut(s) 578, 610
FatI CATG 7 cut(s) 84, 270, 286, 382, 400, 430, 670
FbaI TGATCA 3 cut(s) 376, 409, 433
Fnu4HI GCNGC 6 cut(s) 25, 28, 258, 261, 362, 591
FokI GGATG 1 cut(s) 105
Fsp4HI GCNGC 6 cut(s) 25, 28, 258, 261, 362, 591
GluI GCNGC 6 cut(s) 25, 28, 258, 261, 362, 591
GsaI CCCAGC 2 cut(s) 169, 239
GsuI CTGGAG 2 cut(s) 282, 645
HaeIII GGCC 1 cut(s) 127
HapII CCGG 4 cut(s) 70, 150, 180, 186
Hin1II CATG 7 cut(s) 88, 274, 290, 386, 404, 434, 674
HpaII CCGG 4 cut(s) 70, 150, 180, 186
HphI GGTGA 3 cut(s) 88, 209, 407
Hpy188I TCNGA 3 cut(s) 109, 487, 562
Hpy188III TCNNGA 4 cut(s) 383, 431, 624, 671
HpyAV CCTTC 1 cut(s) 669
HpyCH4III ACNGT 5 cut(s) 294, 313, 331, 471, 549
HpyCH4V TGCA 5 cut(s) 6, 24, 260, 308, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 370
HpyF3I CTNAG 1 cut(s) 43
Hsp92II CATG 7 cut(s) 88, 274, 290, 386, 404, 434, 674
KroI GCCGGC 1 cut(s) 149
KroNI GCCGGC 1 cut(s) 151
Ksp22I TGATCA 3 cut(s) 376, 409, 433
Kzo9I GATC 8 cut(s) 76, 161, 376, 385, 409, 433, 598, 628
LmnI GCTCC 1 cut(s) 370
Lsp1109I GCAGC 6 cut(s) 36, 39, 244, 272, 348, 577
LweI GCATC 1 cut(s) 11
MaeIII GTNAC 1 cut(s) 11
MalI GATC 8 cut(s) 78, 163, 378, 387, 411, 435, 600, 630
MboI GATC 8 cut(s) 76, 161, 376, 385, 409, 433, 598, 628
MboII GAAGA 3 cut(s) 257, 350, 487
MfeI CAATTG 1 cut(s) 365
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 1 cut(s) 114
MlsI TGGCCA 1 cut(s) 127
MluCI AATT 2 cut(s) 365, 513
MluNI TGGCCA 1 cut(s) 127
MmeI TCCRAC 2 cut(s) 295, 498
MnlI CCTC 5 cut(s) 208, 340, 481, 545, 675
Mox20I TGGCCA 1 cut(s) 127
MroNI GCCGGC 1 cut(s) 149
MroXI GAANNNNTTC 1 cut(s) 322
MscI TGGCCA 1 cut(s) 127
MseI TTAA 2 cut(s) 303, 333
MslI CAYNNNNRTG 2 cut(s) 399, 405
Msp20I TGGCCA 1 cut(s) 127
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 4 cut(s) 70, 150, 180, 186
MspR9I CCNGG 1 cut(s) 124
MunI CAATTG 1 cut(s) 365
MvaI CCWGG 1 cut(s) 124
MwoI GCNNNNNNNGC 1 cut(s) 370
NaeI GCCGGC 1 cut(s) 151
NdeII GATC 8 cut(s) 76, 161, 376, 385, 409, 433, 598, 628
NgoMIV GCCGGC 1 cut(s) 149
NlaIII CATG 7 cut(s) 88, 274, 290, 386, 404, 434, 674
NlaIV GGNNCC 2 cut(s) 184, 600
NspI RCATGY 1 cut(s) 88
PagI TCATGA 3 cut(s) 382, 430, 670
PciI ACATGT 1 cut(s) 84
PdiI GCCGGC 1 cut(s) 151
PdmI GAANNNNTTC 1 cut(s) 322
PkrI GCNGC 6 cut(s) 26, 29, 259, 262, 363, 592
PscI ACATGT 1 cut(s) 84
PsiI TTATAA 1 cut(s) 691
Psp6I CCWGG 1 cut(s) 122
PspFI CCCAGC 2 cut(s) 165, 235
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 2 cut(s) 184, 600
PstI CTGCAG 1 cut(s) 262
PsuI RGATCY 1 cut(s) 598
PvuII CAGCTG 1 cut(s) 263
RsaI GTAC 1 cut(s) 291
RsaNI GTAC 1 cut(s) 290
RseI CAYNNNNRTG 2 cut(s) 399, 405
SaqAI TTAA 2 cut(s) 303, 333
SatI GCNGC 6 cut(s) 25, 28, 258, 261, 362, 591
Sau3AI GATC 8 cut(s) 76, 161, 376, 385, 409, 433, 598, 628
ScrFI CCNGG 1 cut(s) 124
SduI GDGCHC 1 cut(s) 114
SfaNI GCATC 1 cut(s) 11
SfcI CTRYAG 2 cut(s) 16, 258
SmiMI CAYNNNNRTG 2 cut(s) 399, 405
Sse9I AATT 2 cut(s) 365, 513
StyD4I CCNGG 1 cut(s) 122
StyI CCWWGG 1 cut(s) 594
TaaI ACNGT 5 cut(s) 294, 313, 331, 471, 549
TasI AATT 2 cut(s) 365, 513
TatI WGTACW 1 cut(s) 289
Tru1I TTAA 2 cut(s) 303, 333
Tru9I TTAA 2 cut(s) 303, 333
TscAI CASTG 1 cut(s) 554
TseI GCWGC 6 cut(s) 24, 27, 257, 260, 361, 590
TspDTI ATGAA 4 cut(s) 287, 579, 651, 687
TspRI CASTG 1 cut(s) 554
XceI RCATGY 1 cut(s) 88
XmnI GAANNNNTTC 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.