Rroxscaffold_1G00059580
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010362
Physical Location & Seq
Reverse (-)
81551389 .. 81554371
2983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00059580.1

Sequence Viewer

Length: 1413 bp
ATGTGCAGCTTGTCTATGCCAATTGGGATGGAGAATTATCAAGGCGATTTAACTGACATACTTCGAGCTGGTGCTGCTGCTGCTGCTACAGGATCATCCTCATCATCGGCTTATCATTCCGAAACAATGAATTTCGACAGCTGGCAGTTTTCATCCGATGATAACTATTCTATGAAATTCTCGACATCAATGGCCATGGAAGTAGTAGAAGACGCAAGGGACAATTTCGGCGATCCGTTTTCATCGTACATACGCGATCCACTTCTTCACGAGCTGTCGTCCACTTTCAACGGAGGCTTTTTCAGCGGCCCAAATTCGACTCCAGAAATGAACATCAGTGTAGATATGGAAGCTCGTGCTGCGCCGTTTGGTGCTCATGTGCCTGGTCACCATCATCAAGGTGAGGTTACTAAGAGGCCTTGCAACAACATATTCTCTCGTATGCTTCAGATCTCCCCCGGGAAGCTAGGCGGCGACGTGGTGGGGTGTGATTCAGTGGCGGTGTTGGGGTCACCCAGGGGGGAGGAGGAGGAGGAATCAAGGCGGGGCCTGATCTCATCTCCACGGAATCCGGGTATCAAGCGAAGGAAGAGTCAGGCGAAGAAGGTGGTATGTATTCCAGCGCCAGCAGCAGCAAACAGTAGGCCAACTGGAGAAGTAGTTCCATCTGATCTGTGGGCATGGAGAAAGTACGGTCAGAAACCCATCAAAGGTTCACCTTATCCAAGAGGTTACTATAGGTGCAGCAGCTCAAAGGGTTGCTCAGCAAGGAAACAAGTAGAGCGTAGCCGGACTGATCCAAACATGTTGGTCATCACCTACACTTCCGAGCACAACCATCCCTGGCCAACCCAGAGAAATGCTCTCGCCGGCTCAACCCGATCCCAGCCATCAAAAGCCGGTGCCGGTTCAAAGGCCTCTTCTAATACTGCTCATCACCAAAAGCCAGATAGCCCAGCAAAGGAAGAACAAAAGGCTGCAGCTGACAGCATGAACGAGAATAATCATGTACTGTCTCCAGTTAATGCAACTGTTGGAAGTAGTTCAACAGTTAAAGAAGAGTTTGAGGACATTGAGAAGCTGCAATTGGAGCTTGATCATCATGATCCCCATCATCAGCATGATAGTGATCATCACCAGTTCAGTAATCATGATCAAGGTTTTCCTTATAGGCCATCAATGCCTGAACAGTCCAACAACCAATCAGAGGATTTCTTCGCTGATTTAGGAGAAATTGAAGCTGACCCACTAAACCTCTTGTTCTCACAGTGTTTCACAGCAGATGAACAGCAAAAGGGAGGAAACAAGGCTGCCTTGGATCCATTCAATCTCTTTGATTGGTCTGGAGATCAAAACACCAACACCCCCAACAACAATCCTTCATTTGGTCATGAAGCCAAGAGGCGTTTATAA

Protein Analysis

470

Amino Acids

51.57

Weight (kDa)

5.85

Isoelectric Point (pI)

54.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 224 - 281 4.5e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1411
AccB1I GGYRCC 1 cut(s) 902
AccII CGCG 1 cut(s) 255
AciI CCGC 4 cut(s) 306, 471, 500, 544
AclWI GGATC 8 cut(s) 100, 227, 251, 791, 876, 1100, 1313, 1326
AcoI YGGCCR 2 cut(s) 192, 845
AcsI RAATTY 3 cut(s) 130, 176, 313
AcuI CTGAAG 1 cut(s) 431
AfaI GTAC 3 cut(s) 248, 692, 1011
AfiI CCNNNNNNNGG 4 cut(s) 710, 961, 1207, 1385
AflIII ACRYGT 1 cut(s) 804
AgsI TTSAA 5 cut(s) 289, 912, 1047, 1238, 1327
AjiI CACGTC 1 cut(s) 478
AjnI CCWGG 3 cut(s) 382, 515, 842
AjuI GAANNNNNNNTTGG 2 cut(s) 1070, 1102
AloI GAACNNNNNNTCC 2 cut(s) 645, 677
Alw21I GWGCWC 2 cut(s) 376, 834
Alw26I GTCTC 1 cut(s) 1020
AlwI GGATC 8 cut(s) 100, 227, 251, 791, 876, 1100, 1313, 1326
Ama87I CYCGRG 1 cut(s) 458
AoxI GGCC 8 cut(s) 192, 307, 416, 547, 644, 845, 915, 1172
ApoI RAATTY 3 cut(s) 130, 176, 313
ArsI GACNNNNNNTTYG 2 cut(s) 577, 609
Asp700I GAANNNNTTC 2 cut(s) 660, 1042
AspLEI GCGC 2 cut(s) 364, 625
AspS9I GGNCC 2 cut(s) 308, 547
AsuC2I CCSGG 3 cut(s) 459, 460, 573
AsuHPI GGTGA 7 cut(s) 380, 413, 504, 708, 808, 929, 1127
AvaI CYCGRG 1 cut(s) 458
BalI TGGCCA 2 cut(s) 194, 847
BamHI GGATCC 1 cut(s) 1318
BanI GGYRCC 1 cut(s) 902
BauI CACGAG 2 cut(s) 269, 354
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 2 cut(s) 376, 834
BccI CCATC 8 cut(s) 22, 399, 673, 713, 846, 898, 1119, 1183
BceAI ACGGC 1 cut(s) 349
BciT130I CCWGG 3 cut(s) 384, 517, 844
BclI TGATCA 3 cut(s) 1096, 1129, 1153
BcnI CCSGG 3 cut(s) 459, 460, 573
BcoDI GTCTC 1 cut(s) 1020
BfaI CTAG 1 cut(s) 467
BfmI CTRYAG 3 cut(s) 87, 736, 978
BfoI RGCGCY 1 cut(s) 626
BglII AGATCT 1 cut(s) 450
BlpI GCTNAGC 1 cut(s) 763
Bme1390I CCNGG 6 cut(s) 384, 459, 460, 517, 573, 844
BmeT110I CYCGRG 1 cut(s) 458
BmgBI CACGTC 1 cut(s) 478
BmgT120I GGNCC 2 cut(s) 308, 547
BmiI GGNNCC 3 cut(s) 548, 904, 1320
BmrFI CCNGG 6 cut(s) 384, 459, 460, 517, 573, 844
BpiI GAAGAC 1 cut(s) 216
BplI GAGNNNNNCTC 2 cut(s) 847, 879
BpmI CTGGAG 4 cut(s) 306, 672, 1002, 1365
Bpu1102I GCTNAGC 1 cut(s) 763
BpuMI CCSGG 3 cut(s) 459, 460, 573
BsaBI GATNNNNATC 2 cut(s) 1110, 1128
BsaJI CCNNGG 8 cut(s) 195, 457, 458, 515, 516, 563, 842, 1314
BsaXI ACNNNNNCTCC 2 cut(s) 645, 675
Bsc4I CCNNNNNNNGG 4 cut(s) 710, 961, 1207, 1385
Bse118I RCCGGY 3 cut(s) 869, 899, 905
Bse1I ACTGG 3 cut(s) 655, 1019, 1138
Bse8I GATNNNNATC 2 cut(s) 1110, 1128
BseBI CCWGG 3 cut(s) 384, 517, 844
BseDI CCNNGG 8 cut(s) 195, 457, 458, 515, 516, 563, 842, 1314
BseGI GGATG 4 cut(s) 33, 95, 152, 838
BseJI GATNNNNATC 2 cut(s) 1110, 1128
BseLI CCNNNNNNNGG 4 cut(s) 710, 961, 1207, 1385
BseMII CTCAG 1 cut(s) 777
BseNI ACTGG 3 cut(s) 655, 1019, 1138
BseRI GAGGAG 3 cut(s) 539, 542, 545
BseYI CCCAGC 2 cut(s) 885, 955
BsgI GTGCAG 2 cut(s) 25, 763
Bsh1236I CGCG 1 cut(s) 255
BshFI GGCC 8 cut(s) 194, 309, 418, 549, 646, 847, 917, 1174
BshNI GGYRCC 1 cut(s) 902
BsiHKAI GWGCWC 2 cut(s) 376, 834
BsiHKCI CYCGRG 1 cut(s) 458
BsiSI CCGG 6 cut(s) 459, 572, 790, 870, 900, 906
BslFI GGGAC 1 cut(s) 233
BslI CCNNNNNNNGG 4 cut(s) 710, 961, 1207, 1385
BsmAI GTCTC 1 cut(s) 1020
BsmFI GGGAC 1 cut(s) 233
BsnI GGCC 8 cut(s) 194, 309, 418, 549, 646, 847, 917, 1174
BsoBI CYCGRG 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 376, 834
Bsp1720I GCTNAGC 1 cut(s) 763
Bsp19I CCATGG 1 cut(s) 195
BspACI CCGC 4 cut(s) 306, 471, 500, 544
BspANI GGCC 8 cut(s) 194, 309, 418, 549, 646, 847, 917, 1174
BspCNI CTCAG 1 cut(s) 776
BspFNI CGCG 1 cut(s) 255
BspHI TCATGA 3 cut(s) 1102, 1150, 1390
BspLI GGNNCC 3 cut(s) 548, 904, 1320
BspMAI CTGCAG 1 cut(s) 982
BspPI GGATC 8 cut(s) 100, 227, 251, 791, 876, 1100, 1313, 1326
BspT107I GGYRCC 1 cut(s) 902
BsrFI RCCGGY 3 cut(s) 869, 899, 905
BsrI ACTGG 3 cut(s) 655, 1019, 1138
BssAI RCCGGY 3 cut(s) 869, 899, 905
BssECI CCNNGG 8 cut(s) 195, 457, 458, 515, 516, 563, 842, 1314
BssSI CACGAG 2 cut(s) 269, 354
BssT1I CCWWGG 2 cut(s) 195, 1314
Bst2BI CACGAG 2 cut(s) 269, 354
Bst2UI CCWGG 3 cut(s) 384, 517, 844
Bst4CI ACNGT 7 cut(s) 641, 695, 1014, 1033, 1051, 1191, 1269
Bst6I CTCTTC 3 cut(s) 584, 925, 1053
BstC8I GCNNGC 3 cut(s) 143, 627, 871
BstDEI CTNAG 2 cut(s) 411, 763
BstDSI CCRYGG 2 cut(s) 195, 563
BstEII GGTNACC 2 cut(s) 386, 510
BstF5I GGATG 4 cut(s) 33, 95, 152, 838
BstFNI CGCG 1 cut(s) 255
BstH2I RGCGCY 1 cut(s) 626
BstHHI GCGC 2 cut(s) 364, 625
BstMAI GTCTC 1 cut(s) 1020
BstMWI GCNNNNNNNGC 8 cut(s) 74, 80, 83, 303, 359, 629, 1090, 1180
BstNI CCWGG 3 cut(s) 384, 517, 844
BstNSI RCATGY 1 cut(s) 808
BstPI GGTNACC 2 cut(s) 386, 510
BstSCI CCNGG 6 cut(s) 382, 457, 458, 515, 571, 842
BstSFI CTRYAG 3 cut(s) 87, 736, 978
BstUI CGCG 1 cut(s) 255
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 2 cut(s) 450, 1318
BstYI RGATCY 2 cut(s) 450, 1318
BsuRI GGCC 8 cut(s) 194, 309, 418, 549, 646, 847, 917, 1174
BtgI CCRYGG 2 cut(s) 195, 563
BtrI CACGTC 1 cut(s) 478
BtsCI GGATG 4 cut(s) 33, 95, 152, 838
BtsIMutI CAGTG 3 cut(s) 343, 501, 1274
Cac8I GCNNGC 3 cut(s) 143, 627, 871
CciI TCATGA 3 cut(s) 1102, 1150, 1390
CfoI GCGC 2 cut(s) 364, 625
Cfr10I RCCGGY 3 cut(s) 869, 899, 905
Cfr13I GGNCC 2 cut(s) 308, 547
Cfr9I CCCGGG 1 cut(s) 458
CseI GACGC 1 cut(s) 221
Csp6I GTAC 3 cut(s) 247, 691, 1010
CviQI GTAC 3 cut(s) 247, 691, 1010
DdeI CTNAG 2 cut(s) 411, 763
EaeI YGGCCR 2 cut(s) 192, 845
Eam1104I CTCTTC 3 cut(s) 584, 925, 1053
EarI CTCTTC 3 cut(s) 584, 925, 1053
Eco130I CCWWGG 2 cut(s) 195, 1314
Eco147I AGGCCT 2 cut(s) 418, 917
Eco57I CTGAAG 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 458
Eco91I GGTNACC 2 cut(s) 386, 510
EcoO109I RGGNCCY 1 cut(s) 547
EcoO65I GGTNACC 2 cut(s) 386, 510
EcoRII CCWGG 3 cut(s) 382, 515, 842
EcoT14I CCWWGG 2 cut(s) 195, 1314
ErhI CCWWGG 2 cut(s) 195, 1314
FalI AAGNNNNNCTT 2 cut(s) 1298, 1330
FaqI GGGAC 1 cut(s) 233
FauI CCCGC 1 cut(s) 537
FbaI TGATCA 3 cut(s) 1096, 1129, 1153
FokI GGATG 4 cut(s) 40, 82, 139, 825
FspBI CTAG 1 cut(s) 467
GlaI GCGC 2 cut(s) 363, 624
GsaI CCCAGC 2 cut(s) 889, 959
GsuI CTGGAG 4 cut(s) 306, 672, 1002, 1365
HaeII RGCGCY 1 cut(s) 626
HaeIII GGCC 8 cut(s) 194, 309, 418, 549, 646, 847, 917, 1174
HapII CCGG 6 cut(s) 459, 572, 790, 870, 900, 906
HgaI GACGC 1 cut(s) 221
HhaI GCGC 2 cut(s) 364, 625
Hin6I GCGC 2 cut(s) 362, 623
HinP1I GCGC 2 cut(s) 362, 623
HinfI GANTC 5 cut(s) 319, 491, 536, 568, 592
HpaII CCGG 6 cut(s) 459, 572, 790, 870, 900, 906
HphI GGTGA 7 cut(s) 380, 413, 504, 708, 808, 929, 1127
Hpy166II GTNNAC 2 cut(s) 282, 716
Hpy188I TCNGA 7 cut(s) 121, 157, 450, 670, 699, 829, 1207
Hpy188III TCNNGA 7 cut(s) 181, 269, 323, 1103, 1151, 1344, 1391
Hpy8I GTNNAC 2 cut(s) 282, 716
Hpy99I CGWCG 1 cut(s) 479
HpyAV CCTTC 3 cut(s) 579, 598, 1389
HpyCH4III ACNGT 7 cut(s) 641, 695, 1014, 1033, 1051, 1191, 1269
HpyCH4IV ACGT 1 cut(s) 477
HpyCH4V TGCA 6 cut(s) 6, 423, 744, 980, 1028, 1084
HpyF10VI GCNNNNNNNGC 8 cut(s) 74, 80, 83, 303, 359, 629, 1090, 1180
HpyF3I CTNAG 2 cut(s) 411, 763
HpySE526I ACGT 1 cut(s) 477
HspAI GCGC 2 cut(s) 362, 623
KroI GCCGGC 1 cut(s) 869
KroNI GCCGGC 1 cut(s) 871
Ksp22I TGATCA 3 cut(s) 1096, 1129, 1153
LmnI GCTCC 1 cut(s) 1090
MaeI CTAG 1 cut(s) 467
MaeII ACGT 1 cut(s) 477
MaeIII GTNAC 4 cut(s) 386, 406, 510, 731
MboII GAAGA 8 cut(s) 221, 257, 601, 613, 912, 977, 1070, 1207
MfeI CAATTG 2 cut(s) 21, 1085
MflI RGATCY 2 cut(s) 450, 1318
MhlI GDGCHC 2 cut(s) 376, 834
MlsI TGGCCA 2 cut(s) 194, 847
MluCI AATT 8 cut(s) 21, 34, 130, 176, 223, 313, 1085, 1233
MluNI TGGCCA 2 cut(s) 194, 847
MlyI GAGTC 2 cut(s) 313, 601
MmeI TCCRAC 2 cut(s) 1015, 1218
Mox20I TGGCCA 2 cut(s) 194, 847
MroNI GCCGGC 1 cut(s) 869
MroXI GAANNNNTTC 2 cut(s) 660, 1042
MscI TGGCCA 2 cut(s) 194, 847
MseI TTAA 3 cut(s) 50, 1023, 1053
MslI CAYNNNNRTG 3 cut(s) 399, 1119, 1125
Msp20I TGGCCA 2 cut(s) 194, 847
MspA1I CMGCKG 3 cut(s) 141, 306, 983
MspI CCGG 6 cut(s) 459, 572, 790, 870, 900, 906
MspR9I CCNGG 6 cut(s) 384, 459, 460, 517, 573, 844
MunI CAATTG 2 cut(s) 21, 1085
MvaI CCWGG 3 cut(s) 384, 517, 844
MvnI CGCG 1 cut(s) 255
MwoI GCNNNNNNNGC 8 cut(s) 74, 80, 83, 303, 359, 629, 1090, 1180
NaeI GCCGGC 1 cut(s) 871
NciI CCSGG 3 cut(s) 459, 460, 573
NcoI CCATGG 1 cut(s) 195
NgoMIV GCCGGC 1 cut(s) 869
NlaIV GGNNCC 3 cut(s) 548, 904, 1320
NmuCI GTSAC 2 cut(s) 386, 510
NspI RCATGY 1 cut(s) 808
PagI TCATGA 3 cut(s) 1102, 1150, 1390
PasI CCCWGGG 1 cut(s) 516
PceI AGGCCT 2 cut(s) 418, 917
PciI ACATGT 1 cut(s) 804
PdiI GCCGGC 1 cut(s) 871
PdmI GAANNNNTTC 2 cut(s) 660, 1042
PfeI GAWTC 3 cut(s) 491, 536, 568
PleI GAGTC 2 cut(s) 313, 600
PpsI GAGTC 2 cut(s) 313, 600
PscI ACATGT 1 cut(s) 804
PsiI TTATAA 1 cut(s) 1411
Psp6I CCWGG 3 cut(s) 382, 515, 842
PspEI GGTNACC 2 cut(s) 386, 510
PspFI CCCAGC 2 cut(s) 885, 955
PspGI CCWGG 3 cut(s) 382, 515, 842
PspN4I GGNNCC 3 cut(s) 548, 904, 1320
PspPI GGNCC 2 cut(s) 308, 547
PstI CTGCAG 1 cut(s) 982
PsuI RGATCY 2 cut(s) 450, 1318
PvuII CAGCTG 2 cut(s) 141, 983
RsaI GTAC 3 cut(s) 248, 692, 1011
RsaNI GTAC 3 cut(s) 247, 691, 1010
RseI CAYNNNNRTG 3 cut(s) 399, 1119, 1125
SaqAI TTAA 3 cut(s) 50, 1023, 1053
Sau96I GGNCC 2 cut(s) 308, 547
SchI GAGTC 2 cut(s) 313, 601
ScrFI CCNGG 6 cut(s) 384, 459, 460, 517, 573, 844
SduI GDGCHC 2 cut(s) 376, 834
SfcI CTRYAG 3 cut(s) 87, 736, 978
SmaI CCCGGG 1 cut(s) 460
SmiMI CAYNNNNRTG 3 cut(s) 399, 1119, 1125
Sse9I AATT 8 cut(s) 21, 34, 130, 176, 223, 313, 1085, 1233
SseBI AGGCCT 2 cut(s) 418, 917
SsiI CCGC 4 cut(s) 306, 471, 500, 544
SspMI CTAG 1 cut(s) 467
StuI AGGCCT 2 cut(s) 418, 917
StyD4I CCNGG 6 cut(s) 382, 457, 458, 515, 571, 842
StyI CCWWGG 2 cut(s) 195, 1314
TaaI ACNGT 7 cut(s) 641, 695, 1014, 1033, 1051, 1191, 1269
TaiI ACGT 1 cut(s) 480
TaqI TCGA 4 cut(s) 64, 135, 182, 317
TasI AATT 8 cut(s) 21, 34, 130, 176, 223, 313, 1085, 1233
TatI WGTACW 1 cut(s) 1009
TauI GCSGC 2 cut(s) 309, 474
TfiI GAWTC 3 cut(s) 491, 536, 568
Tru1I TTAA 3 cut(s) 50, 1023, 1053
Tru9I TTAA 3 cut(s) 50, 1023, 1053
TscAI CASTG 3 cut(s) 343, 501, 1274
TseFI GTSAC 2 cut(s) 386, 510
Tsp45I GTSAC 2 cut(s) 386, 510
TspDTI ATGAA 9 cut(s) 141, 143, 188, 231, 344, 1007, 1299, 1371, 1407
TspGWI ACGGA 3 cut(s) 225, 306, 580
TspMI CCCGGG 1 cut(s) 458
TspRI CASTG 3 cut(s) 343, 501, 1274
XapI RAATTY 3 cut(s) 130, 176, 313
XceI RCATGY 1 cut(s) 808
XcmI CCANNNNNNNNNTGG 1 cut(s) 672
XmaI CCCGGG 1 cut(s) 458
XmnI GAANNNNTTC 2 cut(s) 660, 1042
XspI CTAG 1 cut(s) 467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.