RLG00000032335
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010362
Physical Location & Seq
Forward (+)
13664325 .. 13667202
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032335

Sequence Viewer

Length: 1506 bp
ATGTGCAGCTTGTCTATGCCAATTGGGATGGAGAATTATCAAGGCGATTTAACTGACATACTTCGAGCTGGTGCTGCTGCTGCTGCTGCTACAGGATCATCCTCATCATCGGCTTATCATTCCGAAACAATGAATTCCGACAGCTGGCAGTTTTCATCCGATGATAACTATTCTATGAAATTCTCGACATCAATGGCCATGGAAGAAGTAGAAGACGCAAGGGACAATTTCGGCGATCCGTTTTCATCGTACATACGCGATCCACTTCTTCACGAGCTGTCGTCCACTTTCACCGGAGGCTTTTTCAGCTGCCCAAATTCGATTCCAGAAATGAACATCAGTGTAGATATGGAAGCTCGTGCTGCGCCGTTTGGTGCTCATGTGCCTGGTCACCATCATCAAGGTGAGGTTGCTAAGAGGCCTTGCAACAACATATTCTCTCGTATGCTTCAGATCTCCCCCGGGAAGCTAGGCGGCGACGTGGCCGGTTGTGATTCGGTGGCGGTGTTGGGGTCACCGAGGGGGGGAGGAGGAGGAGGAGGAGGAATCAAGGCGGGGCCTGGTGGTGTTATGGTTGGAAGCGACATGATTAATGCAAACAGCTCAAAAGGTTGCTTGCTCGAGAACACCGGAGTTGGGGTGCAGATCTCATCTCCACGGAATCCGGGTATCAAGCGAAGGAAGAGTCAGGCGAAGAAGGTGGTATGTATTCCAGCGCCAGCAGCAGCAAACAGTAGGCCAACTGGAGAAGTAGTTCCATCTGATCTGTGGGCATGGAGAAAGTACGGTCAGAAACCCATCAAAGGTTCACCTTATCCAAGAGGTTACTATAGATGCAGCAGCTCAAAGGGTTGCTCAGCAAGGAAACAAGTAGAGCGTAGCCGGACTGATCCAAACATGTTGGTCATCACCTACACTTCCGAGCACAACCATCCCTGGCCAACCCAGAGAAATGCTCTCGCCGGCTCAACCCGATCCCAGCCATCAAAAGCCGGTGCCGGTTCAAAGACCTCTCCTAATACTGCTCATCACCAAAAGCCAGATAGCCCAGCAAAGGAAGAACAGAAGGCTGCAGCTGACAGCATGAACGAGCATAATCATGTACTGTCTCCAGTTCATGCAACTGTTGGAAGTAGTTCAACAGTTAAAGAAGAGTTTGAGGACATTGAGAAGCTGCAATTGGAGCTTGATCATCATGATCCCCATCATCAGCATGATAGTGATCATCACCAGTTCAGTAATCATGATCAAGGTTTTCCTTATAGACCATCAATGCCTGAACAGTCCAACAACCAATCAGAGGATTTCTTCGCTGATTTAGGAGAAATTGAAGCTGACCCACTAAACCTCTTGTTCTCACAGTGTTTCACAGCAGATGAACAGAAAAAGGGAGGAAACAAGGCTGCCTTGGATCCATTCAATCTCTTTGATTGGTCTGGAGATCAAAACACCAACAGCCCCAACAACAATCCTTCATTTGGTCATGAAGCCAAGAGGCGTTTATAA

Protein Analysis

502

Amino Acids

54.04

Weight (kDa)

6.12

Isoelectric Point (pI)

52.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 255 - 312 4.9e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1504
AccB1I GGYRCC 1 cut(s) 995
AccII CGCG 1 cut(s) 258
AciI CCGC 3 cut(s) 474, 503, 554
AclWI GGATC 8 cut(s) 103, 230, 254, 884, 969, 1193, 1406, 1419
AcoI YGGCCR 3 cut(s) 195, 483, 938
AcsI RAATTY 3 cut(s) 133, 179, 316
AcuI CTGAAG 1 cut(s) 434
AfaI GTAC 3 cut(s) 251, 785, 1104
AfiI CCNNNNNNNGG 7 cut(s) 144, 524, 636, 803, 1054, 1300, 1478
AflIII ACRYGT 1 cut(s) 897
AgsI TTSAA 4 cut(s) 1005, 1140, 1331, 1420
AjiI CACGTC 1 cut(s) 481
AjnI CCWGG 3 cut(s) 385, 559, 935
AjuI GAANNNNNNNTTGG 2 cut(s) 1163, 1195
AloI GAACNNNNNNTCC 2 cut(s) 738, 770
Alw21I GWGCWC 2 cut(s) 379, 927
Alw26I GTCTC 1 cut(s) 1113
AlwI GGATC 8 cut(s) 103, 230, 254, 884, 969, 1193, 1406, 1419
Ama87I CYCGRG 2 cut(s) 461, 620
AoxI GGCC 6 cut(s) 195, 419, 483, 557, 737, 938
ApoI RAATTY 3 cut(s) 133, 179, 316
ArsI GACNNNNNNTTYG 2 cut(s) 670, 702
AseI ATTAAT 1 cut(s) 591
Asp700I GAANNNNTTC 2 cut(s) 753, 1135
AspLEI GCGC 2 cut(s) 367, 718
AspS9I GGNCC 1 cut(s) 557
AsuC2I CCSGG 3 cut(s) 462, 463, 666
AsuHPI GGTGA 8 cut(s) 283, 383, 416, 507, 801, 901, 1022, 1220
AvaI CYCGRG 2 cut(s) 461, 620
BalI TGGCCA 2 cut(s) 197, 940
BamHI GGATCC 1 cut(s) 1411
BanI GGYRCC 1 cut(s) 995
BauI CACGAG 2 cut(s) 272, 357
BbsI GAAGAC 1 cut(s) 219
Bbv12I GWGCWC 2 cut(s) 379, 927
BccI CCATC 8 cut(s) 22, 402, 766, 806, 939, 991, 1212, 1276
BceAI ACGGC 1 cut(s) 352
BciT130I CCWGG 3 cut(s) 387, 561, 937
BclI TGATCA 3 cut(s) 1189, 1222, 1246
BcnI CCSGG 3 cut(s) 462, 463, 666
BcoDI GTCTC 1 cut(s) 1113
BfaI CTAG 1 cut(s) 470
BfmI CTRYAG 3 cut(s) 90, 829, 1071
BfoI RGCGCY 1 cut(s) 719
BglII AGATCT 2 cut(s) 453, 645
BlpI GCTNAGC 1 cut(s) 856
Bme1390I CCNGG 6 cut(s) 387, 462, 463, 561, 666, 937
BmeT110I CYCGRG 2 cut(s) 461, 620
BmgBI CACGTC 1 cut(s) 481
BmgT120I GGNCC 1 cut(s) 557
BmiI GGNNCC 3 cut(s) 558, 997, 1413
BmrFI CCNGG 6 cut(s) 387, 462, 463, 561, 666, 937
BmsI GCATC 1 cut(s) 824
BpiI GAAGAC 1 cut(s) 219
BplI GAGNNNNNCTC 2 cut(s) 940, 972
BpmI CTGGAG 3 cut(s) 765, 1095, 1458
Bpu1102I GCTNAGC 1 cut(s) 856
BpuMI CCSGG 3 cut(s) 462, 463, 666
BsaBI GATNNNNATC 2 cut(s) 1203, 1221
BsaJI CCNNGG 7 cut(s) 198, 460, 461, 518, 656, 935, 1407
BsaWI WCCGGW 2 cut(s) 293, 629
BsaXI ACNNNNNCTCC 4 cut(s) 624, 654, 738, 768
Bsc4I CCNNNNNNNGG 7 cut(s) 144, 524, 636, 803, 1054, 1300, 1478
Bse118I RCCGGY 4 cut(s) 485, 962, 992, 998
Bse1I ACTGG 3 cut(s) 748, 1112, 1231
Bse8I GATNNNNATC 2 cut(s) 1203, 1221
BseBI CCWGG 3 cut(s) 387, 561, 937
BseDI CCNNGG 7 cut(s) 198, 460, 461, 518, 656, 935, 1407
BseGI GGATG 4 cut(s) 33, 98, 155, 931
BseJI GATNNNNATC 2 cut(s) 1203, 1221
BseLI CCNNNNNNNGG 7 cut(s) 144, 524, 636, 803, 1054, 1300, 1478
BseMII CTCAG 1 cut(s) 870
BseNI ACTGG 3 cut(s) 748, 1112, 1231
BseRI GAGGAG 5 cut(s) 543, 546, 549, 552, 555
BseYI CCCAGC 2 cut(s) 978, 1048
BsgI GTGCAG 2 cut(s) 25, 662
Bsh1236I CGCG 1 cut(s) 258
BshFI GGCC 6 cut(s) 197, 421, 485, 559, 739, 940
BshNI GGYRCC 1 cut(s) 995
BsiHKAI GWGCWC 2 cut(s) 379, 927
BsiHKCI CYCGRG 2 cut(s) 461, 620
BsiSI CCGG 9 cut(s) 294, 462, 486, 630, 665, 883, 963, 993, 999
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 7 cut(s) 144, 524, 636, 803, 1054, 1300, 1478
BsmAI GTCTC 1 cut(s) 1113
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 6 cut(s) 197, 421, 485, 559, 739, 940
BsoBI CYCGRG 2 cut(s) 461, 620
Bsp1286I GDGCHC 2 cut(s) 379, 927
Bsp1720I GCTNAGC 1 cut(s) 856
Bsp19I CCATGG 1 cut(s) 198
BspACI CCGC 3 cut(s) 474, 503, 554
BspANI GGCC 6 cut(s) 197, 421, 485, 559, 739, 940
BspCNI CTCAG 1 cut(s) 869
BspFNI CGCG 1 cut(s) 258
BspHI TCATGA 3 cut(s) 1195, 1243, 1483
BspLI GGNNCC 3 cut(s) 558, 997, 1413
BspMAI CTGCAG 1 cut(s) 1075
BspPI GGATC 8 cut(s) 103, 230, 254, 884, 969, 1193, 1406, 1419
BspT107I GGYRCC 1 cut(s) 995
BsrFI RCCGGY 4 cut(s) 485, 962, 992, 998
BsrI ACTGG 3 cut(s) 748, 1112, 1231
BssAI RCCGGY 4 cut(s) 485, 962, 992, 998
BssECI CCNNGG 7 cut(s) 198, 460, 461, 518, 656, 935, 1407
BssSI CACGAG 2 cut(s) 272, 357
BssT1I CCWWGG 2 cut(s) 198, 1407
Bst2BI CACGAG 2 cut(s) 272, 357
Bst2UI CCWGG 3 cut(s) 387, 561, 937
Bst4CI ACNGT 7 cut(s) 734, 788, 1107, 1126, 1144, 1284, 1362
Bst6I CTCTTC 2 cut(s) 677, 1146
BstC8I GCNNGC 4 cut(s) 146, 617, 720, 964
BstDEI CTNAG 2 cut(s) 414, 856
BstDSI CCRYGG 2 cut(s) 198, 656
BstEII GGTNACC 2 cut(s) 389, 513
BstF5I GGATG 4 cut(s) 33, 98, 155, 931
BstFNI CGCG 1 cut(s) 258
BstH2I RGCGCY 1 cut(s) 719
BstHHI GCGC 2 cut(s) 367, 718
BstMAI GTCTC 1 cut(s) 1113
BstMWI GCNNNNNNNGC 8 cut(s) 74, 80, 83, 86, 306, 362, 722, 1183
BstNI CCWGG 3 cut(s) 387, 561, 937
BstNSI RCATGY 1 cut(s) 901
BstPI GGTNACC 2 cut(s) 389, 513
BstSCI CCNGG 6 cut(s) 385, 460, 461, 559, 664, 935
BstSFI CTRYAG 3 cut(s) 90, 829, 1071
BstUI CGCG 1 cut(s) 258
BstV2I GAAGAC 1 cut(s) 219
BstX2I RGATCY 3 cut(s) 453, 645, 1411
BstYI RGATCY 3 cut(s) 453, 645, 1411
BsuRI GGCC 6 cut(s) 197, 421, 485, 559, 739, 940
BtgI CCRYGG 2 cut(s) 198, 656
BtrI CACGTC 1 cut(s) 481
BtsCI GGATG 4 cut(s) 33, 98, 155, 931
BtsIMutI CAGTG 2 cut(s) 346, 1367
Cac8I GCNNGC 4 cut(s) 146, 617, 720, 964
CciI TCATGA 3 cut(s) 1195, 1243, 1483
CfoI GCGC 2 cut(s) 367, 718
Cfr10I RCCGGY 4 cut(s) 485, 962, 992, 998
Cfr13I GGNCC 1 cut(s) 557
Cfr9I CCCGGG 1 cut(s) 461
CseI GACGC 1 cut(s) 224
Csp6I GTAC 3 cut(s) 250, 784, 1103
CviQI GTAC 3 cut(s) 250, 784, 1103
DdeI CTNAG 2 cut(s) 414, 856
EaeI YGGCCR 3 cut(s) 195, 483, 938
Eam1104I CTCTTC 2 cut(s) 677, 1146
EarI CTCTTC 2 cut(s) 677, 1146
Eco130I CCWWGG 2 cut(s) 198, 1407
Eco147I AGGCCT 1 cut(s) 421
Eco57I CTGAAG 1 cut(s) 434
Eco88I CYCGRG 2 cut(s) 461, 620
Eco91I GGTNACC 2 cut(s) 389, 513
EcoO109I RGGNCCY 1 cut(s) 557
EcoO65I GGTNACC 2 cut(s) 389, 513
EcoRI GAATTC 1 cut(s) 133
EcoRII CCWGG 3 cut(s) 385, 559, 935
EcoT14I CCWWGG 2 cut(s) 198, 1407
ErhI CCWWGG 2 cut(s) 198, 1407
FalI AAGNNNNNCTT 2 cut(s) 1391, 1423
FaqI GGGAC 1 cut(s) 236
FauI CCCGC 1 cut(s) 547
FbaI TGATCA 3 cut(s) 1189, 1222, 1246
FokI GGATG 4 cut(s) 40, 85, 142, 918
FspBI CTAG 1 cut(s) 470
GlaI GCGC 2 cut(s) 366, 717
GsaI CCCAGC 2 cut(s) 982, 1052
GsuI CTGGAG 3 cut(s) 765, 1095, 1458
HaeII RGCGCY 1 cut(s) 719
HaeIII GGCC 6 cut(s) 197, 421, 485, 559, 739, 940
HapII CCGG 9 cut(s) 294, 462, 486, 630, 665, 883, 963, 993, 999
HgaI GACGC 1 cut(s) 224
HhaI GCGC 2 cut(s) 367, 718
Hin6I GCGC 2 cut(s) 365, 716
HinP1I GCGC 2 cut(s) 365, 716
HinfI GANTC 5 cut(s) 322, 494, 546, 661, 685
HpaII CCGG 9 cut(s) 294, 462, 486, 630, 665, 883, 963, 993, 999
HphI GGTGA 8 cut(s) 283, 383, 416, 507, 801, 901, 1022, 1220
Hpy166II GTNNAC 2 cut(s) 285, 809
Hpy188I TCNGA 8 cut(s) 124, 139, 160, 453, 763, 792, 922, 1300
Hpy188III TCNNGA 8 cut(s) 184, 272, 326, 622, 1196, 1244, 1437, 1484
Hpy8I GTNNAC 2 cut(s) 285, 809
Hpy99I CGWCG 1 cut(s) 482
HpyAV CCTTC 4 cut(s) 672, 691, 1060, 1482
HpyCH4III ACNGT 7 cut(s) 734, 788, 1107, 1126, 1144, 1284, 1362
HpyCH4IV ACGT 1 cut(s) 480
HpyCH4V TGCA 8 cut(s) 6, 426, 596, 643, 837, 1073, 1121, 1177
HpyF10VI GCNNNNNNNGC 8 cut(s) 74, 80, 83, 86, 306, 362, 722, 1183
HpyF3I CTNAG 2 cut(s) 414, 856
HpySE526I ACGT 1 cut(s) 480
HspAI GCGC 2 cut(s) 365, 716
KroI GCCGGC 1 cut(s) 962
KroNI GCCGGC 1 cut(s) 964
Ksp22I TGATCA 3 cut(s) 1189, 1222, 1246
LmnI GCTCC 1 cut(s) 1183
LweI GCATC 1 cut(s) 824
MaeI CTAG 1 cut(s) 470
MaeII ACGT 1 cut(s) 480
MaeIII GTNAC 3 cut(s) 389, 513, 824
MboII GAAGA 8 cut(s) 215, 224, 260, 694, 706, 1070, 1163, 1300
MfeI CAATTG 2 cut(s) 21, 1178
MflI RGATCY 3 cut(s) 453, 645, 1411
MhlI GDGCHC 2 cut(s) 379, 927
MlsI TGGCCA 2 cut(s) 197, 940
MluCI AATT 8 cut(s) 21, 34, 133, 179, 226, 316, 1178, 1326
MluNI TGGCCA 2 cut(s) 197, 940
MlyI GAGTC 1 cut(s) 694
MmeI TCCRAC 4 cut(s) 162, 556, 1108, 1311
Mox20I TGGCCA 2 cut(s) 197, 940
MroNI GCCGGC 1 cut(s) 962
MroXI GAANNNNTTC 2 cut(s) 753, 1135
MscI TGGCCA 2 cut(s) 197, 940
MseI TTAA 3 cut(s) 50, 591, 1146
MslI CAYNNNNRTG 4 cut(s) 402, 1098, 1212, 1218
Msp20I TGGCCA 2 cut(s) 197, 940
MspA1I CMGCKG 3 cut(s) 144, 309, 1076
MspI CCGG 9 cut(s) 294, 462, 486, 630, 665, 883, 963, 993, 999
MspR9I CCNGG 6 cut(s) 387, 462, 463, 561, 666, 937
MunI CAATTG 2 cut(s) 21, 1178
MvaI CCWGG 3 cut(s) 387, 561, 937
MvnI CGCG 1 cut(s) 258
MwoI GCNNNNNNNGC 8 cut(s) 74, 80, 83, 86, 306, 362, 722, 1183
NaeI GCCGGC 1 cut(s) 964
NciI CCSGG 3 cut(s) 462, 463, 666
NcoI CCATGG 1 cut(s) 198
NgoMIV GCCGGC 1 cut(s) 962
NlaIV GGNNCC 3 cut(s) 558, 997, 1413
NmuCI GTSAC 2 cut(s) 389, 513
NspI RCATGY 1 cut(s) 901
PaeR7I CTCGAG 1 cut(s) 620
PagI TCATGA 3 cut(s) 1195, 1243, 1483
PceI AGGCCT 1 cut(s) 421
PciI ACATGT 1 cut(s) 897
PdiI GCCGGC 1 cut(s) 964
PdmI GAANNNNTTC 2 cut(s) 753, 1135
PfeI GAWTC 4 cut(s) 322, 494, 546, 661
PleI GAGTC 1 cut(s) 693
PpsI GAGTC 1 cut(s) 693
PscI ACATGT 1 cut(s) 897
PshBI ATTAAT 1 cut(s) 591
PsiI TTATAA 1 cut(s) 1504
Psp6I CCWGG 3 cut(s) 385, 559, 935
PspEI GGTNACC 2 cut(s) 389, 513
PspFI CCCAGC 2 cut(s) 978, 1048
PspGI CCWGG 3 cut(s) 385, 559, 935
PspN4I GGNNCC 3 cut(s) 558, 997, 1413
PspPI GGNCC 1 cut(s) 557
PstI CTGCAG 1 cut(s) 1075
PsuI RGATCY 3 cut(s) 453, 645, 1411
PvuII CAGCTG 3 cut(s) 144, 309, 1076
RsaI GTAC 3 cut(s) 251, 785, 1104
RsaNI GTAC 3 cut(s) 250, 784, 1103
RseI CAYNNNNRTG 4 cut(s) 402, 1098, 1212, 1218
SaqAI TTAA 3 cut(s) 50, 591, 1146
Sau96I GGNCC 1 cut(s) 557
SchI GAGTC 1 cut(s) 694
ScrFI CCNGG 6 cut(s) 387, 462, 463, 561, 666, 937
SduI GDGCHC 2 cut(s) 379, 927
SfaNI GCATC 1 cut(s) 824
SfcI CTRYAG 3 cut(s) 90, 829, 1071
Sfr274I CTCGAG 1 cut(s) 620
SlaI CTCGAG 1 cut(s) 620
SmaI CCCGGG 1 cut(s) 463
SmiMI CAYNNNNRTG 4 cut(s) 402, 1098, 1212, 1218
SmlI CTYRAG 1 cut(s) 620
SmoI CTYRAG 1 cut(s) 620
Sse9I AATT 8 cut(s) 21, 34, 133, 179, 226, 316, 1178, 1326
SseBI AGGCCT 1 cut(s) 421
SsiI CCGC 3 cut(s) 474, 503, 554
SspMI CTAG 1 cut(s) 470
StuI AGGCCT 1 cut(s) 421
StyD4I CCNGG 6 cut(s) 385, 460, 461, 559, 664, 935
StyI CCWWGG 2 cut(s) 198, 1407
TaaI ACNGT 7 cut(s) 734, 788, 1107, 1126, 1144, 1284, 1362
TaiI ACGT 1 cut(s) 483
TaqI TCGA 4 cut(s) 64, 185, 320, 621
TasI AATT 8 cut(s) 21, 34, 133, 179, 226, 316, 1178, 1326
TatI WGTACW 1 cut(s) 1102
TauI GCSGC 1 cut(s) 477
TfiI GAWTC 4 cut(s) 322, 494, 546, 661
Tru1I TTAA 3 cut(s) 50, 591, 1146
Tru9I TTAA 3 cut(s) 50, 591, 1146
TscAI CASTG 2 cut(s) 346, 1367
TseFI GTSAC 2 cut(s) 389, 513
Tsp45I GTSAC 2 cut(s) 389, 513
TspGWI ACGGA 2 cut(s) 228, 673
TspMI CCCGGG 1 cut(s) 461
TspRI CASTG 2 cut(s) 346, 1367
VspI ATTAAT 1 cut(s) 591
XapI RAATTY 3 cut(s) 133, 179, 316
XceI RCATGY 1 cut(s) 901
XcmI CCANNNNNNNNNTGG 1 cut(s) 765
XhoI CTCGAG 1 cut(s) 620
XmaI CCCGGG 1 cut(s) 461
XmnI GAANNNNTTC 2 cut(s) 753, 1135
XspI CTAG 1 cut(s) 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.