MD10G1243000.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010362
Physical Location & Seq
Forward (+)
33776504 .. 33781157
4654 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1243000.v1.1.491

Sequence Viewer

Length: 1482 bp
ATGTGCAGCTTGTTCGTGCCAATTACTAGGATGGAGAATTATCAAGGTGATTTAACCGACATACTAAGAGCTGGTACAACTACTGCTAACGTTGGAGCTTATCCGTCTGATCATCAAGAACCTCACGCCGACACGTGGCACTTCCCATCTGATCATCCCATGATATTTCCATCATCTTCATCAGCCATGGAAGAAGAAGAAGCTAAGGACAACTTTGGCGATCCATTTTCGTCGTACATGCGCGATCCGCTTCTTAACGAGCTAGACATATCAAGCTCCAGCCTTTTCAGCAGCCCCAGTTGTACAGCTCGTGTGGAGGAAGGTGTCACAACTACTACTTTTGGTGGCGGTGGCGGTGGCGGTGGCGGTCATCATGTACTTAGTCTTGGGCATCACAACCATCAAAGTGGTGTTGTTGTGGTTGATGGTGATCCAATAAAGAGGCCTTGCAACATATTCTCACAGATGCTACAGATCTCTCCAAGTGCAAAGGTACCCATGTCGCCGTGTGATTCGCCGGTGATCGGTGGAGCAGTGTCGTCGTCTCCTAGGGGGATAAAAACCTCACCAGATTCTGCTGCCATGATTGCAAGCGACATGATTATTAATGCGAACAGCTCATCAGCAGCAAAAGGTTGCTTGCTCGCGAACACCGGGATGCAGATCTCATCTCCACGGAATCCGGGGATCAAGCGAAGGAAGAGCCAGGCAAAGAAGGTGGTGTGCATTCCAGCGCCAGCGGCTGCAAACAGCAGGCCTACTGGAGAAGTAGTTCCGTCTGATCTGTGGGCATGGAGAAAGTACGGTCAGAAACCCATCAAAGGGTCACCTTTTCCAAGGGGCTACTACAGATGTAGCAGCTCAAAGGGTTGCCCAGCAAGAAAACAAGTAGAGCGTAGCCGGACGGATCCAAACATGTTGGTAATCACCTACACTTCTGAACACAATCATCCATGGCCTACTCAGAGAAATGCCCTAGCTGGCTCAACCAGATCCCAGCCATCCAAAAACGGCGCCGCCGCCGCCTTGAAAGTCTCTCCCAGTAGCTCTCATCAGGCTCATCAAAAGCCAACTAGCCCAACAAAGGAAGAACAAAATGAGAATATGAATGATGATAACCATGTGTCTCCAACAGTTATCACCGCTGGAAGTAATTCAACAGTGAAGGAAGAATTTGAGGGCATTGAGAAGCAATTAGAGATTGATCATCATCAGCAGCACTTAGGGTTTGCTTATAGACCATCAATGCCTAACGAGCAGTCCAACCAGTCTCACCATCCTGAGGACTTCTTTGCTGATTTAGGAGAAATTGAGGCTGACCCACTAAACCTCTTGTTTTCACAATGTTTCTCAGCGGATGAGCAGCAGCAAAAGGGAGGCAAGGATGCAGCGCCCTCAGATCCATTCAATCTCTTTGATAATTGGTCAGGAGATCACAACACCAACAATACTTCATTTGGTGAAGCTAAGAGGCAGTTATAA

Protein Analysis

494

Amino Acids

53.24

Weight (kDa)

6.05

Isoelectric Point (pI)

61.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 261 - 318 2e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1480
Acc65I GGTACC 1 cut(s) 493
AccB1I GGYRCC 2 cut(s) 493, 1013
AccII CGCG 2 cut(s) 243, 647
AclI AACGTT 1 cut(s) 90
AclWI GGATC 8 cut(s) 215, 239, 425, 695, 902, 915, 987, 1394
AcsI RAATTY 1 cut(s) 1172
AcvI CACGTG 1 cut(s) 135
AcyI GRCGYC 1 cut(s) 1014
AfaI GTAC 6 cut(s) 76, 236, 304, 378, 495, 803
AfiI CCNNNNNNNGG 6 cut(s) 135, 524, 821, 822, 1084, 1282
AflIII ACRYGT 2 cut(s) 132, 915
AgsI TTSAA 3 cut(s) 1030, 1158, 1408
AjnI CCWGG 1 cut(s) 705
AloI GAACNNNNNNTCC 2 cut(s) 756, 788
Alw26I GTCTC 4 cut(s) 549, 1039, 1131, 1275
AlwI GGATC 8 cut(s) 215, 239, 425, 695, 902, 915, 987, 1394
AlwNI CAGNNNCTG 2 cut(s) 575, 743
AoxI GGCC 3 cut(s) 443, 755, 956
ApoI RAATTY 1 cut(s) 1172
AseI ATTAAT 1 cut(s) 606
Asp700I GAANNNNTTC 2 cut(s) 771, 1153
Asp718I GGTACC 1 cut(s) 493
AspA2I CCTAGG 1 cut(s) 548
AspLEI GCGC 4 cut(s) 243, 736, 1016, 1393
AsuC2I CCSGG 2 cut(s) 655, 684
AsuHPI GGTGA 9 cut(s) 59, 440, 532, 558, 819, 919, 1132, 1265, 1472
AvrII CCTAGG 1 cut(s) 548
AxyI CCTNAGG 1 cut(s) 1281
BaeI ACNNNNGTAYC 2 cut(s) 485, 518
BamHI GGATCC 1 cut(s) 907
BanI GGYRCC 2 cut(s) 493, 1013
BauI CACGAG 1 cut(s) 309
BbrPI CACGTG 1 cut(s) 135
BccI CCATC 9 cut(s) 25, 154, 178, 408, 419, 824, 1009, 1249, 1284
BceAI ACGGC 2 cut(s) 490, 1027
BcgI CGANNNNNNTGC 3 cut(s) 29, 522, 556
BciT130I CCWGG 1 cut(s) 707
BclI TGATCA 3 cut(s) 109, 151, 1204
BcnI CCSGG 2 cut(s) 655, 684
BcoDI GTCTC 4 cut(s) 549, 1039, 1131, 1275
BfaI CTAG 5 cut(s) 27, 263, 549, 977, 1074
BfmI CTRYAG 2 cut(s) 470, 847
BfoI RGCGCY 3 cut(s) 737, 1017, 1394
BglII AGATCT 2 cut(s) 474, 663
BlnI CCTAGG 1 cut(s) 548
Bme1390I CCNGG 3 cut(s) 655, 684, 707
BmiI GGNNCC 3 cut(s) 495, 909, 1015
BmrFI CCNGG 3 cut(s) 655, 684, 707
BmrI ACTGGG 2 cut(s) 291, 1035
BmsI GCATC 4 cut(s) 400, 456, 648, 1375
BmuI ACTGGG 2 cut(s) 291, 1035
BpmI CTGGAG 2 cut(s) 262, 783
Bpu10I CCTNAGC 1 cut(s) 204
BpuMI CCSGG 2 cut(s) 655, 684
BsaAI YACGTR 1 cut(s) 135
BsaBI GATNNNNATC 3 cut(s) 429, 662, 1209
BsaHI GRCGYC 1 cut(s) 1014
BsaJI CCNNGG 6 cut(s) 186, 548, 674, 683, 836, 953
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 6 cut(s) 135, 524, 821, 822, 1084, 1282
Bse118I RCCGGY 1 cut(s) 517
Bse1I ACTGG 4 cut(s) 297, 766, 1041, 1267
Bse21I CCTNAGG 1 cut(s) 1281
Bse8I GATNNNNATC 3 cut(s) 429, 662, 1209
BseBI CCWGG 1 cut(s) 707
BseDI CCNNGG 6 cut(s) 186, 548, 674, 683, 836, 953
BseGI GGATG 8 cut(s) 36, 154, 663, 949, 1001, 1276, 1363, 1390
BseJI GATNNNNATC 3 cut(s) 429, 662, 1209
BseLI CCNNNNNNNGG 6 cut(s) 135, 524, 821, 822, 1084, 1282
BseMII CTCAG 4 cut(s) 977, 1272, 1365, 1410
BseNI ACTGG 4 cut(s) 297, 766, 1041, 1267
BseYI CCCAGC 2 cut(s) 874, 996
BsgI GTGCAG 1 cut(s) 25
Bsh1236I CGCG 2 cut(s) 243, 647
BshFI GGCC 3 cut(s) 445, 757, 958
BshNI GGYRCC 2 cut(s) 493, 1013
BsiSI CCGG 4 cut(s) 518, 654, 683, 901
BslI CCNNNNNNNGG 6 cut(s) 135, 524, 821, 822, 1084, 1282
BsmAI GTCTC 4 cut(s) 549, 1039, 1131, 1275
BsmBI CGTCTC 1 cut(s) 549
BsmI GAATGC 1 cut(s) 726
BsnI GGCC 3 cut(s) 445, 757, 958
Bsp1407I TGTACA 1 cut(s) 302
Bsp19I CCATGG 2 cut(s) 186, 953
Bsp68I TCGCGA 1 cut(s) 647
BspANI GGCC 3 cut(s) 445, 757, 958
BspCNI CTCAG 4 cut(s) 976, 1273, 1364, 1409
BspFNI CGCG 2 cut(s) 243, 647
BspLI GGNNCC 3 cut(s) 495, 909, 1015
BspPI GGATC 8 cut(s) 215, 239, 425, 695, 902, 915, 987, 1394
BspQI GCTCTTC 1 cut(s) 695
BspT107I GGYRCC 2 cut(s) 493, 1013
BsrFI RCCGGY 1 cut(s) 517
BsrGI TGTACA 1 cut(s) 302
BsrI ACTGG 4 cut(s) 297, 766, 1041, 1267
BssAI RCCGGY 1 cut(s) 517
BssECI CCNNGG 6 cut(s) 186, 548, 674, 683, 836, 953
BssNI GRCGYC 1 cut(s) 1014
BssSI CACGAG 1 cut(s) 309
BssT1I CCWWGG 4 cut(s) 186, 548, 836, 953
Bst2BI CACGAG 1 cut(s) 309
Bst2UI CCWGG 1 cut(s) 707
Bst4CI ACNGT 3 cut(s) 806, 1135, 1162
Bst6I CTCTTC 1 cut(s) 695
BstACI GRCGYC 1 cut(s) 1014
BstAUI TGTACA 1 cut(s) 302
BstBAI YACGTR 1 cut(s) 135
BstC8I GCNNGC 6 cut(s) 592, 641, 645, 738, 755, 982
BstDEI CTNAG 9 cut(s) 65, 204, 380, 963, 1222, 1281, 1351, 1396, 1467
BstDSI CCRYGG 3 cut(s) 186, 674, 953
BstEII GGTNACC 1 cut(s) 825
BstF5I GGATG 8 cut(s) 36, 154, 663, 949, 1001, 1276, 1363, 1390
BstFNI CGCG 2 cut(s) 243, 647
BstH2I RGCGCY 3 cut(s) 737, 1017, 1394
BstHHI GCGC 4 cut(s) 243, 736, 1016, 1393
BstMAI GTCTC 4 cut(s) 549, 1039, 1131, 1275
BstMWI GCNNNNNNNGC 6 cut(s) 247, 288, 587, 740, 1022, 1255
BstNI CCWGG 1 cut(s) 707
BstNSI RCATGY 2 cut(s) 241, 919
BstPI GGTNACC 1 cut(s) 825
BstSCI CCNGG 3 cut(s) 653, 682, 705
BstSFI CTRYAG 2 cut(s) 470, 847
BstUI CGCG 2 cut(s) 243, 647
BstX2I RGATCY 5 cut(s) 474, 663, 907, 992, 1399
BstXI CCANNNNNNTGG 1 cut(s) 407
BstYI RGATCY 5 cut(s) 474, 663, 907, 992, 1399
Bsu36I CCTNAGG 1 cut(s) 1281
BsuRI GGCC 3 cut(s) 445, 757, 958
BtgI CCRYGG 3 cut(s) 186, 674, 953
BtsCI GGATG 8 cut(s) 36, 154, 663, 949, 1001, 1276, 1363, 1390
BtsI GCAGTG 1 cut(s) 540
BtsIMutI CAGTG 2 cut(s) 540, 1167
BtuMI TCGCGA 1 cut(s) 647
Cac8I GCNNGC 6 cut(s) 592, 641, 645, 738, 755, 982
CaiI CAGNNNCTG 2 cut(s) 575, 743
CfoI GCGC 4 cut(s) 243, 736, 1016, 1393
Cfr10I RCCGGY 1 cut(s) 517
Csp6I GTAC 6 cut(s) 75, 235, 303, 377, 494, 802
CspCI CAANNNNNGTGG 2 cut(s) 699, 734
CviQI GTAC 6 cut(s) 75, 235, 303, 377, 494, 802
DdeI CTNAG 9 cut(s) 65, 204, 380, 963, 1222, 1281, 1351, 1396, 1467
DinI GGCGCC 1 cut(s) 1015
Eam1104I CTCTTC 1 cut(s) 695
EarI CTCTTC 1 cut(s) 695
Eco130I CCWWGG 4 cut(s) 186, 548, 836, 953
Eco147I AGGCCT 2 cut(s) 445, 757
Eco72I CACGTG 1 cut(s) 135
Eco81I CCTNAGG 1 cut(s) 1281
Eco91I GGTNACC 1 cut(s) 825
EcoO65I GGTNACC 1 cut(s) 825
EcoRII CCWGG 1 cut(s) 705
EcoT14I CCWWGG 4 cut(s) 186, 548, 836, 953
EgeI GGCGCC 1 cut(s) 1015
EheI GGCGCC 1 cut(s) 1015
ErhI CCWWGG 4 cut(s) 186, 548, 836, 953
Esp3I CGTCTC 1 cut(s) 549
FalI AAGNNNNNCTT 2 cut(s) 197, 229
FbaI TGATCA 3 cut(s) 109, 151, 1204
FokI GGATG 8 cut(s) 43, 141, 670, 936, 988, 1263, 1370, 1397
FspBI CTAG 5 cut(s) 27, 263, 549, 977, 1074
GlaI GCGC 4 cut(s) 242, 735, 1015, 1392
GsaI CCCAGC 2 cut(s) 878, 1000
GsuI CTGGAG 2 cut(s) 262, 783
HaeII RGCGCY 3 cut(s) 737, 1017, 1394
HaeIII GGCC 3 cut(s) 445, 757, 958
HapII CCGG 4 cut(s) 518, 654, 683, 901
HhaI GCGC 4 cut(s) 243, 736, 1016, 1393
Hin1I GRCGYC 1 cut(s) 1014
Hin6I GCGC 4 cut(s) 241, 734, 1014, 1391
HinP1I GCGC 4 cut(s) 241, 734, 1014, 1391
HinfI GANTC 3 cut(s) 512, 572, 679
HpaII CCGG 4 cut(s) 518, 654, 683, 901
HphI GGTGA 9 cut(s) 59, 440, 532, 558, 819, 919, 1132, 1265, 1472
Hpy188I TCNGA 7 cut(s) 109, 151, 781, 810, 940, 966, 1399
Hpy188III TCNNGA 4 cut(s) 116, 646, 1280, 1428
Hpy99I CGWCG 2 cut(s) 235, 544
HpyAV CCTTC 4 cut(s) 314, 690, 709, 1159
HpyCH4III ACNGT 3 cut(s) 806, 1135, 1162
HpyCH4IV ACGT 2 cut(s) 90, 134
HpyCH4V TGCA 8 cut(s) 6, 450, 488, 590, 661, 726, 746, 1388
HpyF10VI GCNNNNNNNGC 6 cut(s) 247, 288, 587, 740, 1022, 1255
HpyF3I CTNAG 9 cut(s) 65, 204, 380, 963, 1222, 1281, 1351, 1396, 1467
HpySE526I ACGT 2 cut(s) 90, 134
Hsp92I GRCGYC 1 cut(s) 1014
HspAI GCGC 4 cut(s) 241, 734, 1014, 1391
KasI GGCGCC 1 cut(s) 1013
KpnI GGTACC 1 cut(s) 497
Ksp22I TGATCA 3 cut(s) 109, 151, 1204
LguI GCTCTTC 1 cut(s) 695
LmnI GCTCC 3 cut(s) 95, 281, 530
LweI GCATC 4 cut(s) 400, 456, 648, 1375
MaeI CTAG 5 cut(s) 27, 263, 549, 977, 1074
MaeII ACGT 2 cut(s) 90, 134
MaeIII GTNAC 2 cut(s) 325, 825
MboII GAAGA 7 cut(s) 168, 203, 206, 209, 712, 1100, 1181
MflI RGATCY 5 cut(s) 474, 663, 907, 992, 1399
MluCI AATT 7 cut(s) 21, 37, 1153, 1172, 1193, 1308, 1420
Mly113I GGCGCC 1 cut(s) 1014
MmeI TCCRAC 3 cut(s) 73, 1154, 1287
MroXI GAANNNNTTC 2 cut(s) 771, 1153
MseI TTAA 3 cut(s) 53, 255, 606
MslI CAYNNNNRTG 2 cut(s) 405, 656
MspA1I CMGCKG 3 cut(s) 740, 1145, 1355
MspI CCGG 4 cut(s) 518, 654, 683, 901
MspR9I CCNGG 3 cut(s) 655, 684, 707
Mva1269I GAATGC 1 cut(s) 726
MvaI CCWGG 1 cut(s) 707
MvnI CGCG 2 cut(s) 243, 647
MwoI GCNNNNNNNGC 6 cut(s) 247, 288, 587, 740, 1022, 1255
NarI GGCGCC 1 cut(s) 1014
NciI CCSGG 2 cut(s) 655, 684
NcoI CCATGG 2 cut(s) 186, 953
NlaIV GGNNCC 3 cut(s) 495, 909, 1015
NmuCI GTSAC 2 cut(s) 325, 825
NruI TCGCGA 1 cut(s) 647
NspI RCATGY 2 cut(s) 241, 919
PceI AGGCCT 2 cut(s) 445, 757
PciI ACATGT 1 cut(s) 915
PciSI GCTCTTC 1 cut(s) 695
PctI GAATGC 1 cut(s) 726
PdmI GAANNNNTTC 2 cut(s) 771, 1153
PfeI GAWTC 3 cut(s) 512, 572, 679
PluTI GGCGCC 1 cut(s) 1017
PmaCI CACGTG 1 cut(s) 135
PmlI CACGTG 1 cut(s) 135
Ppu21I YACGTR 1 cut(s) 135
PscI ACATGT 1 cut(s) 915
PshBI ATTAAT 1 cut(s) 606
PsiI TTATAA 1 cut(s) 1480
Psp1406I AACGTT 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 705
PspCI CACGTG 1 cut(s) 135
PspEI GGTNACC 1 cut(s) 825
PspFI CCCAGC 2 cut(s) 874, 996
PspGI CCWGG 1 cut(s) 705
PspN4I GGNNCC 3 cut(s) 495, 909, 1015
PstNI CAGNNNCTG 2 cut(s) 575, 743
PsuI RGATCY 5 cut(s) 474, 663, 907, 992, 1399
RruI TCGCGA 1 cut(s) 647
RsaI GTAC 6 cut(s) 76, 236, 304, 378, 495, 803
RsaNI GTAC 6 cut(s) 75, 235, 303, 377, 494, 802
RseI CAYNNNNRTG 2 cut(s) 405, 656
SapI GCTCTTC 1 cut(s) 695
SaqAI TTAA 3 cut(s) 53, 255, 606
ScrFI CCNGG 3 cut(s) 655, 684, 707
SfaNI GCATC 4 cut(s) 400, 456, 648, 1375
SfcI CTRYAG 2 cut(s) 470, 847
SfoI GGCGCC 1 cut(s) 1015
SgrAI CRCCGGYG 1 cut(s) 517
SmiMI CAYNNNNRTG 2 cut(s) 405, 656
Sse9I AATT 7 cut(s) 21, 37, 1153, 1172, 1193, 1308, 1420
SseBI AGGCCT 2 cut(s) 445, 757
SspDI GGCGCC 1 cut(s) 1013
SspMI CTAG 5 cut(s) 27, 263, 549, 977, 1074
StuI AGGCCT 2 cut(s) 445, 757
StyD4I CCNGG 3 cut(s) 653, 682, 705
StyI CCWWGG 4 cut(s) 186, 548, 836, 953
TaaI ACNGT 3 cut(s) 806, 1135, 1162
TaiI ACGT 2 cut(s) 93, 137
TasI AATT 7 cut(s) 21, 37, 1153, 1172, 1193, 1308, 1420
TatI WGTACW 2 cut(s) 302, 376
TauI GCSGC 4 cut(s) 743, 1019, 1022, 1025
TfiI GAWTC 3 cut(s) 512, 572, 679
Tru1I TTAA 3 cut(s) 53, 255, 606
Tru9I TTAA 3 cut(s) 53, 255, 606
TscAI CASTG 2 cut(s) 540, 1167
TseFI GTSAC 2 cut(s) 325, 825
Tsp45I GTSAC 2 cut(s) 325, 825
TspDTI ATGAA 3 cut(s) 168, 1121, 1443
TspGWI ACGGA 4 cut(s) 93, 691, 765, 920
TspRI CASTG 2 cut(s) 540, 1167
VspI ATTAAT 1 cut(s) 606
XapI RAATTY 1 cut(s) 1172
XceI RCATGY 2 cut(s) 241, 919
XmaJI CCTAGG 1 cut(s) 548
XmnI GAANNNNTTC 2 cut(s) 771, 1153
XspI CTAG 5 cut(s) 27, 263, 549, 977, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.