Rh5BG131100
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010362
Physical Location & Seq
Forward (+)
12955694 .. 12956368
675 bp
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UTR
Exon/CDS
Intron
Rh5BG131100.1

Sequence Viewer

Length: 675 bp
ATGTGCAGCTTGTCTATGCCAATTGGGATGGAGAATTATCAAGGCGATTTAACTGACATACTTCGAGCTGGTGCTGCTACAGGATCATCCTCATCATCGGCTTATCATTCCGAAACAATGAATTCCGACAGCTGGCAGTTTTCATCCGATGATAACTATTCTATGAAATTCTCGACATCAATGGCCATGGAAGAAGTAGAAGACGCAAGGGACAATTTCGGCGATCCGTTTTCATCGTACATACGCGATCCACTTCTTCACGAGCTGTCGTCCACTTCCACCGGCGGCTTTTTCAGCAGCCCAAATTCGACTCCAGAAATGAACATCAGTGTAGATATGGAAGCTCGTGCTGCGCCGTTTGGTGCTCATGTGCCTGGTCACCATCATCAAGGTGAGGTTACTAAGAGGCCTTGCAACAACATATTCTCTCGTATGCTTCAGATCTCCCCCGGGAAGCTAGGCGGCGACGTGGCGGGGTGTGATTCGGTGGCGGTGTTGGGGTCACCGAGGGGGGGAGGAGGAGGAGGAGGAGGAGGAATCAAGGCGGTGCCTGGTGGTGTTATGGTTGGAAGCGACATGATTAATGCAAACAGCTCAAAAGGTTGCTTGCTCGAGAACACCGGAGTTGGGGTGCAGATCTCATCTCCACGGAATCCGGGTATCAAGCGAAGGTAA

Protein Analysis

224

Amino Acids

23.35

Weight (kDa)

5.28

Isoelectric Point (pI)

52.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010362)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30650 AT2G34830 AT2G34830
fragaria_vesca FvH4_3g11140
malus_domestica MD05G1265200.v1.1 MD10G1243000.v1.1
prunus_persica Prupe.4G101100_v2.0.a1
pyrus_communis pycom05g24920 pycom05g24930 pycom10g20330
rosa_chinensis RchiOBHm_Chr5g0018041
rosa_laevigata RLG00000032335
rosa_multiflora Rmu_sc0010280.1_g000014
rosa_roxburghii Rroxscaffold_1G00059580
rosa_rugosa Rorug05G0040000
rosa_samantha Rh5AG132800 Rh5BG131100 Rh5BG131300 Rh5CG143300 Rh5DG133000
rosa_wichuraiana Rw5G011560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 547
AccII CGCG 1 cut(s) 246
AciI CCGC 5 cut(s) 285, 462, 473, 491, 545
AclWI GGATC 3 cut(s) 91, 218, 242
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 3 cut(s) 121, 167, 304
AcuI CTGAAG 1 cut(s) 422
AfaI GTAC 1 cut(s) 239
AfiI CCNNNNNNNGG 3 cut(s) 132, 512, 627
AjiI CACGTC 1 cut(s) 469
AjnI CCWGG 2 cut(s) 373, 550
AluBI AGCT 7 cut(s) 9, 68, 132, 265, 344, 457, 594
AluI AGCT 7 cut(s) 9, 68, 132, 265, 344, 457, 594
Alw21I GWGCWC 1 cut(s) 367
AlwI GGATC 3 cut(s) 91, 218, 242
Ama87I CYCGRG 2 cut(s) 449, 611
AoxI GGCC 2 cut(s) 183, 407
ApeKI GCWGC 4 cut(s) 6, 74, 297, 350
ApoI RAATTY 3 cut(s) 121, 167, 304
AseI ATTAAT 1 cut(s) 582
AspLEI GCGC 1 cut(s) 355
AsuC2I CCSGG 3 cut(s) 450, 451, 657
AsuHPI GGTGA 3 cut(s) 371, 404, 495
AvaI CYCGRG 2 cut(s) 449, 611
BalI TGGCCA 1 cut(s) 185
BanI GGYRCC 1 cut(s) 547
BauI CACGAG 2 cut(s) 260, 345
BbsI GAAGAC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 367
BbvI GCAGC 4 cut(s) 18, 61, 309, 337
BccI CCATC 2 cut(s) 22, 390
BceAI ACGGC 1 cut(s) 340
BciT130I CCWGG 2 cut(s) 375, 552
BcnI CCSGG 3 cut(s) 450, 451, 657
BfaI CTAG 1 cut(s) 458
BfmI CTRYAG 1 cut(s) 78
BglII AGATCT 2 cut(s) 441, 636
BisI GCNGC 6 cut(s) 7, 75, 286, 298, 351, 463
BlsI GCNGC 6 cut(s) 8, 76, 287, 299, 352, 464
Bme1390I CCNGG 5 cut(s) 375, 450, 451, 552, 657
BmeT110I CYCGRG 2 cut(s) 449, 611
BmgBI CACGTC 1 cut(s) 469
BmiI GGNNCC 1 cut(s) 549
BmrFI CCNGG 5 cut(s) 375, 450, 451, 552, 657
BpiI GAAGAC 1 cut(s) 207
BpmI CTGGAG 1 cut(s) 297
BpuMI CCSGG 3 cut(s) 450, 451, 657
BsaJI CCNNGG 5 cut(s) 186, 448, 449, 506, 647
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 2 cut(s) 615, 645
Bsc4I CCNNNNNNNGG 3 cut(s) 132, 512, 627
Bse118I RCCGGY 1 cut(s) 281
BseBI CCWGG 2 cut(s) 375, 552
BseDI CCNNGG 5 cut(s) 186, 448, 449, 506, 647
BseGI GGATG 3 cut(s) 33, 86, 143
BseLI CCNNNNNNNGG 3 cut(s) 132, 512, 627
BseRI GAGGAG 6 cut(s) 531, 534, 537, 540, 543, 546
BseXI GCAGC 4 cut(s) 18, 61, 309, 337
BsgI GTGCAG 2 cut(s) 25, 653
Bsh1236I CGCG 1 cut(s) 246
BshFI GGCC 2 cut(s) 185, 409
BshNI GGYRCC 1 cut(s) 547
BsiHKAI GWGCWC 1 cut(s) 367
BsiHKCI CYCGRG 2 cut(s) 449, 611
BsiSI CCGG 4 cut(s) 282, 450, 621, 656
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 3 cut(s) 132, 512, 627
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 2 cut(s) 185, 409
BsoBI CYCGRG 2 cut(s) 449, 611
Bsp1286I GDGCHC 1 cut(s) 367
Bsp143I GATC 5 cut(s) 83, 223, 247, 441, 636
Bsp19I CCATGG 1 cut(s) 186
BspACI CCGC 5 cut(s) 285, 462, 473, 491, 545
BspANI GGCC 2 cut(s) 185, 409
BspFNI CGCG 1 cut(s) 246
BspLI GGNNCC 1 cut(s) 549
BspPI GGATC 3 cut(s) 91, 218, 242
BspT107I GGYRCC 1 cut(s) 547
BsrFI RCCGGY 1 cut(s) 281
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 5 cut(s) 186, 448, 449, 506, 647
BssMI GATC 5 cut(s) 83, 223, 247, 441, 636
BssSI CACGAG 2 cut(s) 260, 345
BssT1I CCWWGG 1 cut(s) 186
Bst2BI CACGAG 2 cut(s) 260, 345
Bst2UI CCWGG 2 cut(s) 375, 552
BstC8I GCNNGC 2 cut(s) 134, 608
BstDEI CTNAG 1 cut(s) 402
BstDSI CCRYGG 2 cut(s) 186, 647
BstEII GGTNACC 2 cut(s) 377, 501
BstF5I GGATG 3 cut(s) 33, 86, 143
BstFNI CGCG 1 cut(s) 246
BstHHI GCGC 1 cut(s) 355
BstKTI GATC 5 cut(s) 86, 226, 250, 444, 639
BstMBI GATC 5 cut(s) 83, 223, 247, 441, 636
BstMWI GCNNNNNNNGC 3 cut(s) 74, 294, 350
BstNI CCWGG 2 cut(s) 375, 552
BstPI GGTNACC 2 cut(s) 377, 501
BstSCI CCNGG 5 cut(s) 373, 448, 449, 550, 655
BstSFI CTRYAG 1 cut(s) 78
BstUI CGCG 1 cut(s) 246
BstV1I GCAGC 4 cut(s) 18, 61, 309, 337
BstV2I GAAGAC 1 cut(s) 207
BstX2I RGATCY 2 cut(s) 441, 636
BstYI RGATCY 2 cut(s) 441, 636
BsuRI GGCC 2 cut(s) 185, 409
BtgI CCRYGG 2 cut(s) 186, 647
BtrI CACGTC 1 cut(s) 469
BtsCI GGATG 3 cut(s) 33, 86, 143
BtsIMutI CAGTG 1 cut(s) 334
Cac8I GCNNGC 2 cut(s) 134, 608
CfoI GCGC 1 cut(s) 355
Cfr10I RCCGGY 1 cut(s) 281
Cfr9I CCCGGG 1 cut(s) 449
CseI GACGC 1 cut(s) 212
Csp6I GTAC 1 cut(s) 238
CviAII CATG 3 cut(s) 187, 368, 577
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 1 cut(s) 402
DpnI GATC 5 cut(s) 85, 225, 249, 443, 638
DpnII GATC 5 cut(s) 83, 223, 247, 441, 636
EaeI YGGCCR 1 cut(s) 183
Eco130I CCWWGG 1 cut(s) 186
Eco147I AGGCCT 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 422
Eco88I CYCGRG 2 cut(s) 449, 611
Eco91I GGTNACC 2 cut(s) 377, 501
EcoO65I GGTNACC 2 cut(s) 377, 501
EcoRI GAATTC 1 cut(s) 121
EcoRII CCWGG 2 cut(s) 373, 550
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 3 cut(s) 190, 371, 580
FaqI GGGAC 1 cut(s) 224
FatI CATG 3 cut(s) 186, 367, 576
FauI CCCGC 1 cut(s) 466
Fnu4HI GCNGC 6 cut(s) 7, 75, 286, 298, 351, 463
FokI GGATG 3 cut(s) 40, 73, 130
Fsp4HI GCNGC 6 cut(s) 7, 75, 286, 298, 351, 463
FspBI CTAG 1 cut(s) 458
GlaI GCGC 1 cut(s) 354
GluI GCNGC 6 cut(s) 7, 75, 286, 298, 351, 463
GsuI CTGGAG 1 cut(s) 297
HaeIII GGCC 2 cut(s) 185, 409
HapII CCGG 4 cut(s) 282, 450, 621, 656
HgaI GACGC 1 cut(s) 212
HhaI GCGC 1 cut(s) 355
Hin1II CATG 3 cut(s) 190, 371, 580
Hin6I GCGC 1 cut(s) 353
HinP1I GCGC 1 cut(s) 353
HinfI GANTC 4 cut(s) 310, 482, 537, 652
HpaII CCGG 4 cut(s) 282, 450, 621, 656
HphI GGTGA 3 cut(s) 371, 404, 495
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 4 cut(s) 112, 127, 148, 441
Hpy188III TCNNGA 4 cut(s) 172, 260, 314, 613
Hpy8I GTNNAC 1 cut(s) 273
Hpy99I CGWCG 1 cut(s) 470
HpyAV CCTTC 1 cut(s) 663
HpyCH4IV ACGT 1 cut(s) 468
HpyCH4V TGCA 4 cut(s) 6, 414, 587, 634
HpyF10VI GCNNNNNNNGC 3 cut(s) 74, 294, 350
HpyF3I CTNAG 1 cut(s) 402
HpySE526I ACGT 1 cut(s) 468
Hsp92II CATG 3 cut(s) 190, 371, 580
HspAI GCGC 1 cut(s) 353
Kzo9I GATC 5 cut(s) 83, 223, 247, 441, 636
Lsp1109I GCAGC 4 cut(s) 18, 61, 309, 337
MaeI CTAG 1 cut(s) 458
MaeII ACGT 1 cut(s) 468
MaeIII GTNAC 3 cut(s) 377, 397, 501
MalI GATC 5 cut(s) 85, 225, 249, 443, 638
MboI GATC 5 cut(s) 83, 223, 247, 441, 636
MboII GAAGA 3 cut(s) 203, 212, 248
MfeI CAATTG 1 cut(s) 21
MflI RGATCY 2 cut(s) 441, 636
MhlI GDGCHC 1 cut(s) 367
MlsI TGGCCA 1 cut(s) 185
MluCI AATT 6 cut(s) 21, 34, 121, 167, 214, 304
MluNI TGGCCA 1 cut(s) 185
MlyI GAGTC 1 cut(s) 304
MmeI TCCRAC 2 cut(s) 150, 547
Mox20I TGGCCA 1 cut(s) 185
MscI TGGCCA 1 cut(s) 185
MseI TTAA 2 cut(s) 50, 582
MslI CAYNNNNRTG 1 cut(s) 390
Msp20I TGGCCA 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 132
MspI CCGG 4 cut(s) 282, 450, 621, 656
MspR9I CCNGG 5 cut(s) 375, 450, 451, 552, 657
MunI CAATTG 1 cut(s) 21
MvaI CCWGG 2 cut(s) 375, 552
MvnI CGCG 1 cut(s) 246
MwoI GCNNNNNNNGC 3 cut(s) 74, 294, 350
NciI CCSGG 3 cut(s) 450, 451, 657
NcoI CCATGG 1 cut(s) 186
NdeII GATC 5 cut(s) 83, 223, 247, 441, 636
NlaIII CATG 3 cut(s) 190, 371, 580
NlaIV GGNNCC 1 cut(s) 549
NmuCI GTSAC 2 cut(s) 377, 501
PaeR7I CTCGAG 1 cut(s) 611
PceI AGGCCT 1 cut(s) 409
PfeI GAWTC 3 cut(s) 482, 537, 652
PkrI GCNGC 6 cut(s) 8, 76, 287, 299, 352, 464
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PshBI ATTAAT 1 cut(s) 582
Psp6I CCWGG 2 cut(s) 373, 550
PspEI GGTNACC 2 cut(s) 377, 501
PspGI CCWGG 2 cut(s) 373, 550
PspN4I GGNNCC 1 cut(s) 549
PsuI RGATCY 2 cut(s) 441, 636
PvuII CAGCTG 1 cut(s) 132
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
RseI CAYNNNNRTG 1 cut(s) 390
SaqAI TTAA 2 cut(s) 50, 582
SatI GCNGC 6 cut(s) 7, 75, 286, 298, 351, 463
Sau3AI GATC 5 cut(s) 83, 223, 247, 441, 636
SchI GAGTC 1 cut(s) 304
ScrFI CCNGG 5 cut(s) 375, 450, 451, 552, 657
SduI GDGCHC 1 cut(s) 367
SfcI CTRYAG 1 cut(s) 78
Sfr274I CTCGAG 1 cut(s) 611
SgrAI CRCCGGYG 1 cut(s) 281
SlaI CTCGAG 1 cut(s) 611
SmaI CCCGGG 1 cut(s) 451
SmiMI CAYNNNNRTG 1 cut(s) 390
SmlI CTYRAG 1 cut(s) 611
SmoI CTYRAG 1 cut(s) 611
Sse9I AATT 6 cut(s) 21, 34, 121, 167, 214, 304
SseBI AGGCCT 1 cut(s) 409
SsiI CCGC 5 cut(s) 285, 462, 473, 491, 545
SspMI CTAG 1 cut(s) 458
StuI AGGCCT 1 cut(s) 409
StyD4I CCNGG 5 cut(s) 373, 448, 449, 550, 655
StyI CCWWGG 1 cut(s) 186
TaiI ACGT 1 cut(s) 471
TaqI TCGA 4 cut(s) 64, 173, 308, 612
TasI AATT 6 cut(s) 21, 34, 121, 167, 214, 304
TauI GCSGC 2 cut(s) 288, 465
TfiI GAWTC 3 cut(s) 482, 537, 652
Tru1I TTAA 2 cut(s) 50, 582
Tru9I TTAA 2 cut(s) 50, 582
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 2 cut(s) 377, 501
TseI GCWGC 4 cut(s) 6, 74, 297, 350
Tsp45I GTSAC 2 cut(s) 377, 501
TspDTI ATGAA 5 cut(s) 132, 134, 179, 222, 335
TspGWI ACGGA 2 cut(s) 216, 664
TspMI CCCGGG 1 cut(s) 449
TspRI CASTG 1 cut(s) 334
VspI ATTAAT 1 cut(s) 582
XapI RAATTY 3 cut(s) 121, 167, 304
XhoI CTCGAG 1 cut(s) 611
XmaI CCCGGG 1 cut(s) 449
XspI CTAG 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.