AT1G31660

CONTAINS InterPro DOMAIN s Bystin (InterPro IPR007955)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008571
Physical Location & Seq
Reverse (-)
11330924 .. 11333540
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G31660.1

Sequence Viewer

Length: 1335 bp
ATGGCGAAGAAGCGAGATCGAATTGTCAACACTCAACCTTTTATCTCCGATGACGCCTCCGTCGCTTCTTCCAGGAAACGATCAAAGGTTCCAAAAACCCATCAGAAACAGGAAAAGTTGATTGAAGCTGGTATGAGCGAGAAGATCATGAAACAAGCTCTTGCCCAACAAAAGGAAGTTGCTGATGAAGAGAACGCCGAGAGAAACCCTAGTAGCGCCGCTTTTGCTGTTGCCGGCGCTGCGACTGCTGGAGAGGAGCAGAAAATTCTGGAGGAGGAAGAAGATGATATTGATGATTTTGATGGAACCTTTGAAAACCAGAGCCAATTCGATAAGCAAGAGGAGATTAATGAGGATGATGAGAAGTTGTTTGAGTCATTCTTAAATAAGAATGCTCCTCCTCAACGTACGCTTACTGATATCATTATCAAGAAGCTCAAAGATAAGGATGCTGACTTAGCTGAAGAAGAGCGTCCCGATCCTAAAATGGACCCTGCAATAACTAAGTTATATAAAGGTGTTGGCAAGTTTATGAGTGAGTATACAGTTGGGAAATTGCCAAAAGCATTTAAGCTTGTTACTTCAATGGAACACTGGGAGGATGTTTTATACTTGACTGAGCCAGAGAAATGGTCACCTAACGCATTGTATCAAGCCACAAGGATCTTTGCTTCCAACTTGAAAGACAGACAAGTTCAACGGTTTTATAACTATGTTTTGCTTCCGCGAGTGAGAGAGGATATTAGAAAGCACAAGAAGCTGCACTTTGCTCTGTACCAGGCTCTGAAAAAGTCACTGTATAAGCCTAGTGCTTTCAATCAAGGGATACTATTCCCGCTTTGTAAGTCGGGGACATGCAATCTCAGAGAAGCTGTAATTATAGGGAGCATTCTTGAAAAGTGTTCGATCCCTATGCTTCACTCATGTGTTGCCTTGAACAGACTGGCTGAGATGGATTATTGTGGCACAACAAGTTACTTTATAAAAGTTCTTTTGGAGAAGAAATATTGTATGCCGTATCGGGTGCTTGATGCATTGGTTGCTCACTTCATGAGATTTGTTGACGATATAAGAGTTATGCCTGTGATTTGGCACCAGTCTCTTCTTACCTTTGTGCAACGGTACAAGTATGAAATACTAAAGGAAGACAAAGAACATCTCCAAACTCTCCTCCAGAGGCAAAAACACCATCTTGTTACTCCTGAGATTCTGAGAGAGCTTAAGGATAGCAGAAACCGTGGAGAGAAAGAAGATCCTATGGTGGATAATTTTGCCCCAGTACCGGCTAAAGAAGACAGGTTTGACATTCCTGAAGTACCAATGGAAGAAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

444

Amino Acids

51.47

Weight (kDa)

6.04

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 134 - 416 3.2e-128 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 708, 983
AasI GACNNNNNNGTC 1 cut(s) 59
AccB1I GGYRCC 1 cut(s) 1092
AccI GTMKAC 1 cut(s) 542
AccII CGCG 1 cut(s) 727
AciI CCGC 3 cut(s) 219, 725, 836
AclWI GGATC 4 cut(s) 473, 671, 901, 1247
AcsI RAATTY 1 cut(s) 264
AcuI CTGAAG 2 cut(s) 483, 1332
AcyI GRCGYC 1 cut(s) 54
AfaI GTAC 5 cut(s) 409, 776, 1124, 1281, 1317
AfiI CCNNNNNNNGG 2 cut(s) 172, 1282
AflII CTTAAG 1 cut(s) 1220
AgsI TTSAA 8 cut(s) 125, 314, 585, 682, 698, 817, 896, 937
AjnI CCWGG 2 cut(s) 71, 777
AleI CACNNNNGTG 1 cut(s) 924
AluBI AGCT 8 cut(s) 128, 158, 436, 461, 574, 760, 872, 1219
AluI AGCT 8 cut(s) 128, 158, 436, 461, 574, 760, 872, 1219
Alw26I GTCTC 1 cut(s) 1104
AlwI GGATC 4 cut(s) 473, 671, 901, 1247
AlwNI CAGNNNCTG 1 cut(s) 784
ApeKI GCWGC 2 cut(s) 239, 760
ApoI RAATTY 1 cut(s) 264
AseI ATTAAT 1 cut(s) 348
AspLEI GCGC 2 cut(s) 218, 239
AspS9I GGNCC 1 cut(s) 490
AsuHPI GGTGA 1 cut(s) 627
AvaII GGWCC 1 cut(s) 490
BanI GGYRCC 1 cut(s) 1092
BbsI GAAGAC 2 cut(s) 1152, 1299
BbvI GCAGC 2 cut(s) 226, 747
BccI CCATC 4 cut(s) 108, 296, 946, 1197
BceAI ACGGC 1 cut(s) 1000
BcgI CGANNNNNNTGC 2 cut(s) 895, 929
BciT130I CCWGG 2 cut(s) 73, 779
BciVI GTATCC 1 cut(s) 819
BcoDI GTCTC 1 cut(s) 1104
BfaI CTAG 2 cut(s) 210, 807
BfoI RGCGCY 2 cut(s) 219, 240
BfrI CTTAAG 1 cut(s) 1220
BfuI GTATCC 1 cut(s) 819
BisI GCNGC 3 cut(s) 219, 240, 761
BlsI GCNGC 3 cut(s) 220, 241, 762
Bme1390I CCNGG 2 cut(s) 73, 779
Bme18I GGWCC 1 cut(s) 490
BmgT120I GGNCC 1 cut(s) 490
BmiI GGNNCC 4 cut(s) 90, 307, 492, 1094
BmrFI CCNGG 2 cut(s) 73, 779
BmrI ACTGGG 2 cut(s) 604, 1271
BmsI GCATC 2 cut(s) 439, 1021
BmuI ACTGGG 2 cut(s) 604, 1271
BpiI GAAGAC 2 cut(s) 1152, 1299
BpmI CTGGAG 3 cut(s) 270, 290, 1157
BsaHI GRCGYC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 1237
Bsc4I CCNNNNNNNGG 2 cut(s) 172, 1282
Bse118I RCCGGY 2 cut(s) 233, 1282
Bse1I ACTGG 4 cut(s) 599, 948, 1096, 1277
BseBI CCWGG 2 cut(s) 73, 779
BseDI CCNNGG 1 cut(s) 1237
BseGI GGATG 3 cut(s) 361, 454, 607
BseLI CCNNNNNNNGG 2 cut(s) 172, 1282
BseMII CTCAG 5 cut(s) 609, 877, 939, 1194, 1202
BseNI ACTGG 4 cut(s) 599, 948, 1096, 1277
BseRI GAGGAG 6 cut(s) 269, 287, 356, 387, 390, 1160
BseXI GCAGC 2 cut(s) 226, 747
BsgI GTGCAG 1 cut(s) 746
Bsh1236I CGCG 1 cut(s) 727
BshNI GGYRCC 1 cut(s) 1092
BsiSI CCGG 2 cut(s) 234, 1283
BsiWI CGTACG 1 cut(s) 407
BslFI GGGAC 2 cut(s) 459, 865
BslI CCNNNNNNNGG 2 cut(s) 172, 1282
BsmAI GTCTC 1 cut(s) 1104
BsmFI GGGAC 2 cut(s) 459, 865
BsmI GAATGC 2 cut(s) 397, 888
Bsp143I GATC 7 cut(s) 16, 80, 144, 478, 663, 906, 1252
BspACI CCGC 3 cut(s) 219, 725, 836
BspCNI CTCAG 5 cut(s) 610, 876, 940, 1195, 1203
BspFNI CGCG 1 cut(s) 727
BspHI TCATGA 2 cut(s) 147, 1050
BspLI GGNNCC 4 cut(s) 90, 307, 492, 1094
BspPI GGATC 4 cut(s) 473, 671, 901, 1247
BspQI GCTCTTC 1 cut(s) 462
BspT107I GGYRCC 1 cut(s) 1092
BspTI CTTAAG 1 cut(s) 1220
BsrFI RCCGGY 2 cut(s) 233, 1282
BsrI ACTGG 4 cut(s) 599, 948, 1096, 1277
BssAI RCCGGY 2 cut(s) 233, 1282
BssECI CCNNGG 1 cut(s) 1237
BssMI GATC 7 cut(s) 16, 80, 144, 478, 663, 906, 1252
BssNAI GTATAC 1 cut(s) 543
BssNI GRCGYC 1 cut(s) 54
Bst1107I GTATAC 1 cut(s) 543
Bst2UI CCWGG 2 cut(s) 73, 779
Bst4CI ACNGT 5 cut(s) 547, 702, 798, 1122, 1238
Bst6I CTCTTC 3 cut(s) 183, 462, 1107
BstACI GRCGYC 1 cut(s) 54
BstAFI CTTAAG 1 cut(s) 1220
BstAPI GCANNNNNTGC 1 cut(s) 1040
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 7 cut(s) 457, 504, 618, 863, 948, 1203, 1211
BstDSI CCRYGG 1 cut(s) 1237
BstEII GGTNACC 1 cut(s) 633
BstF5I GGATG 3 cut(s) 361, 454, 607
BstFNI CGCG 1 cut(s) 727
BstH2I RGCGCY 2 cut(s) 219, 240
BstHHI GCGC 2 cut(s) 218, 239
BstKTI GATC 7 cut(s) 19, 83, 147, 481, 666, 909, 1255
BstMAI GTCTC 1 cut(s) 1104
BstMBI GATC 7 cut(s) 16, 80, 144, 478, 663, 906, 1252
BstMWI GCNNNNNNNGC 7 cut(s) 62, 224, 239, 245, 458, 757, 1040
BstNI CCWGG 2 cut(s) 73, 779
BstNSI RCATGY 1 cut(s) 858
BstPI GGTNACC 1 cut(s) 633
BstSCI CCNGG 2 cut(s) 71, 777
BstUI CGCG 1 cut(s) 727
BstV1I GCAGC 2 cut(s) 226, 747
BstV2I GAAGAC 2 cut(s) 1152, 1299
BstX2I RGATCY 2 cut(s) 663, 1252
BstXI CCANNNNNNTGG 1 cut(s) 630
BstYI RGATCY 2 cut(s) 663, 1252
BstZ17I GTATAC 1 cut(s) 543
BsuI GTATCC 1 cut(s) 819
BtgI CCRYGG 1 cut(s) 1237
BtsCI GGATG 3 cut(s) 361, 454, 607
BtsIMutI CAGTG 2 cut(s) 592, 794
Cac8I GCNNGC 1 cut(s) 235
CaiI CAGNNNCTG 1 cut(s) 784
CciI TCATGA 2 cut(s) 147, 1050
CfoI GCGC 2 cut(s) 218, 239
Cfr10I RCCGGY 2 cut(s) 233, 1282
Cfr13I GGNCC 1 cut(s) 490
CseI GACGC 2 cut(s) 62, 461
Csp6I GTAC 5 cut(s) 408, 775, 1123, 1280, 1316
CviAII CATG 4 cut(s) 148, 855, 924, 1051
CviQI GTAC 5 cut(s) 408, 775, 1123, 1280, 1316
DdeI CTNAG 7 cut(s) 457, 504, 618, 863, 948, 1203, 1211
DpnI GATC 7 cut(s) 18, 82, 146, 480, 665, 908, 1254
DpnII GATC 7 cut(s) 16, 80, 144, 478, 663, 906, 1252
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
Eam1104I CTCTTC 3 cut(s) 183, 462, 1107
EarI CTCTTC 3 cut(s) 183, 462, 1107
Eco32I GATATC 1 cut(s) 421
Eco47I GGWCC 1 cut(s) 490
Eco57I CTGAAG 2 cut(s) 483, 1332
Eco91I GGTNACC 1 cut(s) 633
EcoO65I GGTNACC 1 cut(s) 633
EcoRII CCWGG 2 cut(s) 71, 777
EcoRV GATATC 1 cut(s) 421
EcoT22I ATGCAT 1 cut(s) 1036
FaeI CATG 4 cut(s) 151, 858, 927, 1054
FalI AAGNNNNNCTT 2 cut(s) 749, 781
FaqI GGGAC 2 cut(s) 459, 865
FatI CATG 4 cut(s) 147, 854, 923, 1050
FauI CCCGC 1 cut(s) 843
FblI GTMKAC 1 cut(s) 542
Fnu4HI GCNGC 3 cut(s) 219, 240, 761
FokI GGATG 3 cut(s) 368, 461, 614
Fsp4HI GCNGC 3 cut(s) 219, 240, 761
FspBI CTAG 2 cut(s) 210, 807
GlaI GCGC 2 cut(s) 217, 238
GluI GCNGC 3 cut(s) 219, 240, 761
GsuI CTGGAG 3 cut(s) 270, 290, 1157
HaeII RGCGCY 2 cut(s) 219, 240
HapII CCGG 2 cut(s) 234, 1283
HgaI GACGC 2 cut(s) 62, 461
HhaI GCGC 2 cut(s) 218, 239
Hin1I GRCGYC 1 cut(s) 54
Hin1II CATG 4 cut(s) 151, 858, 927, 1054
Hin6I GCGC 2 cut(s) 216, 237
HinP1I GCGC 2 cut(s) 216, 237
HincII GTYRAC 2 cut(s) 28, 1063
HindII GTYRAC 2 cut(s) 28, 1063
HindIII AAGCTT 1 cut(s) 572
HinfI GANTC 2 cut(s) 374, 1207
HpaII CCGG 2 cut(s) 234, 1283
HphI GGTGA 1 cut(s) 627
Hpy166II GTNNAC 3 cut(s) 28, 543, 1063
Hpy188I TCNGA 5 cut(s) 49, 105, 786, 866, 1212
Hpy188III TCNNGA 9 cut(s) 148, 269, 430, 476, 893, 1051, 1174, 1202, 1310
Hpy8I GTNNAC 3 cut(s) 28, 543, 1063
Hpy99I CGWCG 1 cut(s) 65
HpyCH4III ACNGT 5 cut(s) 547, 702, 798, 1122, 1238
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 5 cut(s) 497, 763, 858, 1034, 1117
HpyF10VI GCNNNNNNNGC 7 cut(s) 62, 224, 239, 245, 458, 757, 1040
HpyF3I CTNAG 7 cut(s) 457, 504, 618, 863, 948, 1203, 1211
HpySE526I ACGT 1 cut(s) 406
Hsp92I GRCGYC 1 cut(s) 54
Hsp92II CATG 4 cut(s) 151, 858, 927, 1054
HspAI GCGC 2 cut(s) 216, 237
KroI GCCGGC 1 cut(s) 233
KroNI GCCGGC 1 cut(s) 235
Kzo9I GATC 7 cut(s) 16, 80, 144, 478, 663, 906, 1252
LguI GCTCTTC 1 cut(s) 462
LmnI GCTCC 3 cut(s) 256, 400, 885
Lsp1109I GCAGC 2 cut(s) 226, 747
LweI GCATC 2 cut(s) 439, 1021
MaeI CTAG 2 cut(s) 210, 807
MaeII ACGT 1 cut(s) 406
MaeIII GTNAC 5 cut(s) 577, 633, 792, 974, 1195
MalI GATC 7 cut(s) 18, 82, 146, 480, 665, 908, 1254
MboI GATC 7 cut(s) 16, 80, 144, 478, 663, 906, 1252
MflI RGATCY 2 cut(s) 663, 1252
MluCI AATT 6 cut(s) 21, 264, 326, 554, 876, 1267
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 1 cut(s) 699
Mph1103I ATGCAT 1 cut(s) 1036
MroNI GCCGGC 1 cut(s) 233
MseI TTAA 4 cut(s) 348, 383, 570, 1221
MslI CAYNNNNRTG 1 cut(s) 924
MspCI CTTAAG 1 cut(s) 1220
MspI CCGG 2 cut(s) 234, 1283
MspR9I CCNGG 2 cut(s) 73, 779
Mva1269I GAATGC 2 cut(s) 397, 888
MvaI CCWGG 2 cut(s) 73, 779
MvnI CGCG 1 cut(s) 727
MwoI GCNNNNNNNGC 7 cut(s) 62, 224, 239, 245, 458, 757, 1040
NaeI GCCGGC 1 cut(s) 235
NdeII GATC 7 cut(s) 16, 80, 144, 478, 663, 906, 1252
NgoMIV GCCGGC 1 cut(s) 233
NlaIII CATG 4 cut(s) 151, 858, 927, 1054
NlaIV GGNNCC 4 cut(s) 90, 307, 492, 1094
NmeAIII GCCGAG 1 cut(s) 223
NmuCI GTSAC 2 cut(s) 633, 792
NsiI ATGCAT 1 cut(s) 1036
NspI RCATGY 1 cut(s) 858
OliI CACNNNNGTG 1 cut(s) 924
PagI TCATGA 2 cut(s) 147, 1050
PciSI GCTCTTC 1 cut(s) 462
PctI GAATGC 2 cut(s) 397, 888
PdiI GCCGGC 1 cut(s) 235
PfeI GAWTC 1 cut(s) 1207
Pfl23II CGTACG 1 cut(s) 407
PfoI TCCNGGA 1 cut(s) 71
PkrI GCNGC 3 cut(s) 220, 241, 762
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PshBI ATTAAT 1 cut(s) 348
PsiI TTATAA 2 cut(s) 708, 983
Psp6I CCWGG 2 cut(s) 71, 777
PspEI GGTNACC 1 cut(s) 633
PspGI CCWGG 2 cut(s) 71, 777
PspLI CGTACG 1 cut(s) 407
PspN4I GGNNCC 4 cut(s) 90, 307, 492, 1094
PspPI GGNCC 1 cut(s) 490
PstNI CAGNNNCTG 1 cut(s) 784
PsuI RGATCY 2 cut(s) 663, 1252
RsaI GTAC 5 cut(s) 409, 776, 1124, 1281, 1317
RsaNI GTAC 5 cut(s) 408, 775, 1123, 1280, 1316
RseI CAYNNNNRTG 1 cut(s) 924
SapI GCTCTTC 1 cut(s) 462
SaqAI TTAA 4 cut(s) 348, 383, 570, 1221
SatI GCNGC 3 cut(s) 219, 240, 761
Sau3AI GATC 7 cut(s) 16, 80, 144, 478, 663, 906, 1252
Sau96I GGNCC 1 cut(s) 490
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 2 cut(s) 73, 779
SfaNI GCATC 2 cut(s) 439, 1021
SinI GGWCC 1 cut(s) 490
SmiMI CAYNNNNRTG 1 cut(s) 924
SmlI CTYRAG 1 cut(s) 1220
SmoI CTYRAG 1 cut(s) 1220
Sse9I AATT 6 cut(s) 21, 264, 326, 554, 876, 1267
SsiI CCGC 3 cut(s) 219, 725, 836
SspI AATATT 1 cut(s) 1007
SspMI CTAG 2 cut(s) 210, 807
StyD4I CCNGG 2 cut(s) 71, 777
TaaI ACNGT 5 cut(s) 547, 702, 798, 1122, 1238
TaiI ACGT 1 cut(s) 409
TaqI TCGA 3 cut(s) 19, 330, 905
TasI AATT 6 cut(s) 21, 264, 326, 554, 876, 1267
TauI GCSGC 1 cut(s) 221
TfiI GAWTC 1 cut(s) 1207
Tru1I TTAA 4 cut(s) 348, 383, 570, 1221
Tru9I TTAA 4 cut(s) 348, 383, 570, 1221
TscAI CASTG 2 cut(s) 599, 801
TseFI GTSAC 2 cut(s) 633, 792
TseI GCWGC 2 cut(s) 239, 760
Tsp45I GTSAC 2 cut(s) 633, 792
TspDTI ATGAA 4 cut(s) 164, 201, 1039, 1146
TspGWI ACGGA 1 cut(s) 49
TspRI CASTG 2 cut(s) 599, 801
Vha464I CTTAAG 1 cut(s) 1220
VpaK11BI GGWCC 1 cut(s) 490
VspI ATTAAT 1 cut(s) 348
XapI RAATTY 1 cut(s) 264
XceI RCATGY 1 cut(s) 858
XmiI GTMKAC 1 cut(s) 542
XspI CTAG 2 cut(s) 210, 807
Zsp2I ATGCAT 1 cut(s) 1036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.