Rh5BG192900

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0008571
Physical Location & Seq
Forward (+)
21272642 .. 21276505
3864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG192900.1

Sequence Viewer

Length: 966 bp
ATGCCGAAGAAGAGAGGGAGAGAGAGGCATCACAACCCGGAGCCATTTCTGGCCGACGACGATGGCGCCTCTGTGGCCTCCAAGAAGCGGACCAAGTCCAAGCAGCACCAGACCGACGAGACTCTGATTTCGTCGGGCATGAGCGCCAAGATTCTCAAGCAGGCTCAGCTTCAGCAGAGGGAGGTCGAGGACGACGAGAATCGAACCCAAAACCCCAACGCCTTATCGGACATCACCGAAGAGCCGCACGGGGTTGAATCTGATGACGACGACCCCGATGAGTTCGGTGGGTTTCTTGACAATCGCGGCGAGGATGAAGCGTTTAAAGTGACGAACAGGATGACTAAGGAGATTGGGAAGGAGTATGAGAAAGTATTGGGTAAGGCGGCGGTGGAAGAGGATGAGAAATTACTGGATGCATTTCTTTCGAAGGATGCAGGGCGGCAGCTTACTCTTGCAGACCTGATTGTTGCGAGGATTAAAGAGAATGATTCTAATATCACTCCATCAAATGCCAATTCAGATATGAGGCCTTTGCCTAAATTGGATGCAGACACCATAGAAATCTTCAAGGGGGTTGGCCAGTTTCTTAAAAAGTACACGGCTGGAAAGGTTCCCAAACCCTTTAAACACATCCCTTCAATGAATCAATGGGAAGATGTCCTGTATTTAACTGAGCCTGAGAATTGGTCTCCCAATGCGATGTATCAAGCTACAAGAATTTTCGCTTCCAATTTGACTGCGAAGAAGGTAGAACGTTTCTACAAGCTTGTCTTGCTTCCACGTGTGAGAGAAGACATTCGGAAGAACAAGCGCTTGCATTTTGCCCTTTATCAGGCTTTGAAAAAGTCTCTTTACAAGCCTGCTGCCTTTTTCAAAGGAATATTGCTACCTTTGTGCGAGTGTTGCAGTAATGAAGCTAGCAGACATGGAATACTGTGGTACAACAAGGTACCGTATGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

321

Amino Acids

36.63

Weight (kDa)

6.69

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 148 - 306 2.7e-64 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 952
AccB1I GGYRCC 2 cut(s) 65, 952
AccII CGCG 1 cut(s) 306
AciI CCGC 6 cut(s) 88, 245, 306, 386, 389, 442
AclI AACGTT 1 cut(s) 757
AcoI YGGCCR 2 cut(s) 51, 580
AcsI RAATTY 1 cut(s) 720
AcuI CTGAAG 1 cut(s) 155
AcvI CACGTG 1 cut(s) 785
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 3 cut(s) 599, 944, 954
AfeI AGCGCT 1 cut(s) 815
AfiI CCNNNNNNNGG 2 cut(s) 87, 835
AflIII ACRYGT 1 cut(s) 784
AgsI TTSAA 5 cut(s) 257, 571, 642, 844, 877
AluBI AGCT 5 cut(s) 169, 448, 713, 769, 920
AluI AGCT 5 cut(s) 169, 448, 713, 769, 920
Alw26I GTCTC 3 cut(s) 113, 696, 855
Aor51HI AGCGCT 1 cut(s) 815
AoxI GGCC 4 cut(s) 51, 75, 530, 580
ApeKI GCWGC 3 cut(s) 103, 445, 866
ApoI RAATTY 1 cut(s) 720
ArsI GACNNNNNNTTYG 2 cut(s) 112, 144
Asp700I GAANNNNTTC 1 cut(s) 798
Asp718I GGTACC 1 cut(s) 952
AspLEI GCGC 3 cut(s) 68, 146, 816
AspS9I GGNCC 1 cut(s) 90
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 1 cut(s) 226
AsuII TTCGAA 1 cut(s) 428
AsuNHI GCTAGC 1 cut(s) 920
AvaII GGWCC 1 cut(s) 90
BalI TGGCCA 1 cut(s) 582
BanI GGYRCC 2 cut(s) 65, 952
BbrPI CACGTG 1 cut(s) 785
BbsI GAAGAC 1 cut(s) 801
BbvI GCAGC 3 cut(s) 115, 457, 853
BccI CCATC 2 cut(s) 56, 514
BceAI ACGGC 1 cut(s) 618
BcgI CGANNNNNNTGC 2 cut(s) 408, 442
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 3 cut(s) 113, 696, 855
BfaI CTAG 1 cut(s) 921
BfoI RGCGCY 3 cut(s) 69, 147, 817
BglI GCCNNNNNGGC 1 cut(s) 74
BisI GCNGC 7 cut(s) 104, 245, 307, 387, 443, 446, 867
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 7 cut(s) 105, 246, 308, 388, 444, 447, 868
Bme1390I CCNGG 1 cut(s) 38
Bme18I GGWCC 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 90
BmiI GGNNCC 4 cut(s) 42, 67, 615, 954
BmrFI CCNGG 1 cut(s) 38
BmsI GCATC 4 cut(s) 37, 406, 424, 538
BmtI GCTAGC 1 cut(s) 924
BpiI GAAGAC 1 cut(s) 801
Bpu1102I GCTNAGC 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 428
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 785
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 696
Bsc4I CCNNNNNNNGG 2 cut(s) 87, 835
Bse1I ACTGG 2 cut(s) 417, 583
BseGI GGATG 7 cut(s) 319, 345, 406, 421, 439, 553, 633
BseLI CCNNNNNNNGG 2 cut(s) 87, 835
BseMII CTCAG 3 cut(s) 179, 666, 672
BseNI ACTGG 2 cut(s) 417, 583
BseXI GCAGC 3 cut(s) 115, 457, 853
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 4 cut(s) 53, 77, 532, 582
BshNI GGYRCC 2 cut(s) 65, 952
BsiSI CCGG 1 cut(s) 38
BslI CCNNNNNNNGG 2 cut(s) 87, 835
BsmAI GTCTC 3 cut(s) 113, 696, 855
BsnI GGCC 4 cut(s) 53, 77, 532, 582
Bso31I GGTCTC 1 cut(s) 696
Bsp119I TTCGAA 1 cut(s) 428
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 6 cut(s) 88, 245, 306, 386, 389, 442
BspANI GGCC 4 cut(s) 53, 77, 532, 582
BspCNI CTCAG 3 cut(s) 178, 667, 673
BspFNI CGCG 1 cut(s) 306
BspLI GGNNCC 4 cut(s) 42, 67, 615, 954
BspOI GCTAGC 1 cut(s) 924
BspQI GCTCTTC 1 cut(s) 234
BspT104I TTCGAA 1 cut(s) 428
BspT107I GGYRCC 2 cut(s) 65, 952
BspTNI GGTCTC 1 cut(s) 696
BsrI ACTGG 2 cut(s) 417, 583
BssNI GRCGYC 1 cut(s) 66
Bst4CI ACNGT 2 cut(s) 939, 957
Bst6I CTCTTC 3 cut(s) 5, 234, 390
BstACI GRCGYC 1 cut(s) 66
BstBAI YACGTR 1 cut(s) 785
BstBI TTCGAA 1 cut(s) 428
BstC8I GCNNGC 4 cut(s) 162, 818, 864, 922
BstDEI CTNAG 4 cut(s) 165, 345, 675, 681
BstENI CCTNNNNNAGG 1 cut(s) 833
BstF5I GGATG 7 cut(s) 319, 345, 406, 421, 439, 553, 633
BstFNI CGCG 1 cut(s) 306
BstH2I RGCGCY 3 cut(s) 69, 147, 817
BstHHI GCGC 3 cut(s) 68, 146, 816
BstMAI GTCTC 3 cut(s) 113, 696, 855
BstMWI GCNNNNNNNGC 4 cut(s) 74, 166, 775, 906
BstSCI CCNGG 1 cut(s) 36
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 3 cut(s) 115, 457, 853
BstV2I GAAGAC 1 cut(s) 801
BsuRI GGCC 4 cut(s) 53, 77, 532, 582
BtgZI GCGATG 1 cut(s) 716
BtsCI GGATG 7 cut(s) 319, 345, 406, 421, 439, 553, 633
Cac8I GCNNGC 4 cut(s) 162, 818, 864, 922
CfoI GCGC 3 cut(s) 68, 146, 816
Cfr13I GGNCC 1 cut(s) 90
Csp6I GTAC 3 cut(s) 598, 943, 953
CviAII CATG 2 cut(s) 139, 929
CviQI GTAC 3 cut(s) 598, 943, 953
DdeI CTNAG 4 cut(s) 165, 345, 675, 681
DinI GGCGCC 1 cut(s) 67
DraI TTTAAA 2 cut(s) 325, 628
EaeI YGGCCR 2 cut(s) 51, 580
Eam1104I CTCTTC 3 cut(s) 5, 234, 390
EarI CTCTTC 3 cut(s) 5, 234, 390
Eco147I AGGCCT 1 cut(s) 532
Eco31I GGTCTC 1 cut(s) 696
Eco47I GGWCC 1 cut(s) 90
Eco47III AGCGCT 1 cut(s) 815
Eco57I CTGAAG 1 cut(s) 155
Eco72I CACGTG 1 cut(s) 785
EcoNI CCTNNNNNAGG 1 cut(s) 833
EcoT22I ATGCAT 1 cut(s) 421
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
FaeI CATG 2 cut(s) 142, 932
FaiI YATR 6 cut(s) 140, 366, 527, 560, 930, 960
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FatI CATG 2 cut(s) 138, 928
Fnu4HI GCNGC 7 cut(s) 104, 245, 307, 387, 443, 446, 867
FokI GGATG 7 cut(s) 326, 352, 413, 428, 446, 560, 620
Fsp4HI GCNGC 7 cut(s) 104, 245, 307, 387, 443, 446, 867
FspBI CTAG 1 cut(s) 921
GlaI GCGC 3 cut(s) 67, 145, 815
GluI GCNGC 7 cut(s) 104, 245, 307, 387, 443, 446, 867
HaeII RGCGCY 3 cut(s) 69, 147, 817
HaeIII GGCC 4 cut(s) 53, 77, 532, 582
HapII CCGG 1 cut(s) 38
HhaI GCGC 3 cut(s) 68, 146, 816
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 142, 932
Hin6I GCGC 3 cut(s) 66, 144, 814
HinP1I GCGC 3 cut(s) 66, 144, 814
HindIII AAGCTT 1 cut(s) 767
HinfI GANTC 6 cut(s) 121, 151, 199, 257, 491, 646
HpaII CCGG 1 cut(s) 38
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 1 cut(s) 600
Hpy188I TCNGA 5 cut(s) 126, 229, 262, 523, 804
Hpy188III TCNNGA 1 cut(s) 296
Hpy8I GTNNAC 1 cut(s) 600
Hpy99I CGWCG 6 cut(s) 59, 62, 119, 136, 197, 272
HpyAV CCTTC 4 cut(s) 352, 424, 648, 742
HpyCH4III ACNGT 2 cut(s) 939, 957
HpyCH4IV ACGT 2 cut(s) 757, 784
HpyCH4V TGCA 6 cut(s) 419, 437, 458, 551, 820, 909
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 166, 775, 906
HpyF3I CTNAG 4 cut(s) 165, 345, 675, 681
HpySE526I ACGT 2 cut(s) 757, 784
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 142, 932
HspAI GCGC 3 cut(s) 66, 144, 814
KasI GGCGCC 1 cut(s) 65
KpnI GGTACC 1 cut(s) 956
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 1 cut(s) 40
Lsp1109I GCAGC 3 cut(s) 115, 457, 853
LweI GCATC 4 cut(s) 37, 406, 424, 538
MaeI CTAG 1 cut(s) 921
MaeII ACGT 2 cut(s) 757, 784
MaeIII GTNAC 1 cut(s) 328
MboII GAAGA 9 cut(s) 19, 22, 251, 407, 559, 668, 757, 806, 817
MlsI TGGCCA 1 cut(s) 582
MluCI AATT 6 cut(s) 407, 517, 542, 685, 720, 733
MluNI TGGCCA 1 cut(s) 582
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 1 cut(s) 115
Mox20I TGGCCA 1 cut(s) 582
Mph1103I ATGCAT 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 798
MscI TGGCCA 1 cut(s) 582
MseI TTAA 5 cut(s) 324, 480, 591, 627, 671
Msp20I TGGCCA 1 cut(s) 582
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 1 cut(s) 38
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 4 cut(s) 74, 166, 775, 906
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 1 cut(s) 38
NheI GCTAGC 1 cut(s) 920
NlaIII CATG 2 cut(s) 142, 932
NlaIV GGNNCC 4 cut(s) 42, 67, 615, 954
NmuCI GTSAC 1 cut(s) 328
NsiI ATGCAT 1 cut(s) 421
NspV TTCGAA 1 cut(s) 428
PceI AGGCCT 1 cut(s) 532
PciSI GCTCTTC 1 cut(s) 234
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 273
PdmI GAANNNNTTC 1 cut(s) 798
PfeI GAWTC 5 cut(s) 151, 199, 257, 491, 646
PflFI GACNNNGTC 1 cut(s) 94
PkrI GCNGC 7 cut(s) 105, 246, 308, 388, 444, 447, 868
PleI GAGTC 1 cut(s) 115
PluTI GGCGCC 1 cut(s) 69
PmaCI CACGTG 1 cut(s) 785
PmlI CACGTG 1 cut(s) 785
PpsI GAGTC 1 cut(s) 115
Ppu21I YACGTR 1 cut(s) 785
Psp1406I AACGTT 1 cut(s) 757
PspCI CACGTG 1 cut(s) 785
PspN4I GGNNCC 4 cut(s) 42, 67, 615, 954
PspPI GGNCC 1 cut(s) 90
PsyI GACNNNGTC 1 cut(s) 94
RsaI GTAC 3 cut(s) 599, 944, 954
RsaNI GTAC 3 cut(s) 598, 943, 953
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 5 cut(s) 324, 480, 591, 627, 671
SatI GCNGC 7 cut(s) 104, 245, 307, 387, 443, 446, 867
Sau96I GGNCC 1 cut(s) 90
SchI GAGTC 1 cut(s) 115
ScrFI CCNGG 1 cut(s) 38
SfaNI GCATC 4 cut(s) 37, 406, 424, 538
SfoI GGCGCC 1 cut(s) 67
SfuI TTCGAA 1 cut(s) 428
SinI GGWCC 1 cut(s) 90
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 6 cut(s) 407, 517, 542, 685, 720, 733
SseBI AGGCCT 1 cut(s) 532
SsiI CCGC 6 cut(s) 88, 245, 306, 386, 389, 442
SspDI GGCGCC 1 cut(s) 65
SspI AATATT 1 cut(s) 885
SspMI CTAG 1 cut(s) 921
StuI AGGCCT 1 cut(s) 532
StyD4I CCNGG 1 cut(s) 36
TaaI ACNGT 2 cut(s) 939, 957
TaiI ACGT 2 cut(s) 760, 787
TaqI TCGA 3 cut(s) 186, 202, 428
TaqII GACCGA 1 cut(s) 128
TasI AATT 6 cut(s) 407, 517, 542, 685, 720, 733
TatI WGTACW 1 cut(s) 597
TauI GCSGC 4 cut(s) 247, 309, 389, 445
TfiI GAWTC 5 cut(s) 151, 199, 257, 491, 646
Tru1I TTAA 5 cut(s) 324, 480, 591, 627, 671
Tru9I TTAA 5 cut(s) 324, 480, 591, 627, 671
TseFI GTSAC 1 cut(s) 328
TseI GCWGC 3 cut(s) 103, 445, 866
Tsp45I GTSAC 1 cut(s) 328
TspDTI ATGAA 3 cut(s) 330, 659, 930
Tth111I GACNNNGTC 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 90
XagI CCTNNNNNAGG 1 cut(s) 833
XapI RAATTY 1 cut(s) 720
XmnI GAANNNNTTC 1 cut(s) 798
XspI CTAG 1 cut(s) 921
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.