Rh7CG369600

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0008571
Physical Location & Seq
Forward (+)
46430849 .. 46435146
4298 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG369600.1

Sequence Viewer

Length: 864 bp
ATGGATACCCATTACATAGAAATATTCAAGGGGGTTGCACAGTTTATGAAAACATACACGGCTGGCAAGATTACCAAACCATTCAAGTTTATCCCTTCAATGAAACAATGGGAGGATGCCCTCAAGTTGACTAAGCCTGAGAATTGGTCACCACACGCTATGTATGGAGCTACACGAATTCTAGCTTCTAATCTAAGTCCGCAAGCTGCTGAGCGTTTCTACAAGTTTGTCTTGCTCCCACGGGTGAGGGAAGACATTCGTAAGAACAGGCGTTTGCATTTTGCTTTGTATCAGTCTCTAAAGAAGTCTGTGTACAAGCACGCTGCCTTTTTTAAAGGAATACTGTTACCGTTGTGTCAGTCGAGGACGTGCACTTTAAGAGAAGCTGTTGTTGTTGGGAGTATCATAACGAAAGTCCGGATTCCTGTAAATCATGCAGGTGCTGCATTAATGATGCTAGCACAGATGGAATTCAGCGGCACCACAAGTTACTTTATAAAGCTTCTTTTGGAGAAAAAGTTTGCTTTGCAATATCGTGTAATTGAGGCTCTTGTTGCACATTTCATGGGATTTTTGGAGGAACCCAGGATAATGCCTGTGATATGGCATCAGTCACTTCTTGCATTTGCACAGAGGTACAAAAATGCTCTACTAAAGGAGGACAAAGATAAGCTTATAAATCTTCTTGAGGCACAAAATCACCGTGAGGTAACTCCTGAAATAAGAAGGGAACTGTTTCATAGCCGCAATCGTGGTGAAAAGGAGGATGATATTCTGTCAATTGATTCTCCAATGTCTCTGTTCAATACACCTGTCAAAGAAGACATGTTTGATATTCCAGAGGTTCCTATGGAGGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

287

Amino Acids

33.37

Weight (kDa)

9.39

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 2 - 254 6.6e-114 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 497, 677
AarI CACCTGC 1 cut(s) 428
Acc36I ACCTGC 1 cut(s) 428
AccB1I GGYRCC 1 cut(s) 479
AccIII TCCGGA 1 cut(s) 417
AciI CCGC 3 cut(s) 200, 477, 745
AcsI RAATTY 2 cut(s) 177, 470
AfaI GTAC 2 cut(s) 314, 638
AflIII ACRYGT 1 cut(s) 825
AgsI TTSAA 4 cut(s) 28, 85, 99, 805
AjiI CACGTC 1 cut(s) 369
AjnI CCWGG 1 cut(s) 584
AluBI AGCT 6 cut(s) 170, 185, 206, 386, 502, 673
AluI AGCT 6 cut(s) 170, 185, 206, 386, 502, 673
Alw21I GWGCWC 1 cut(s) 374
Alw26I GTCTC 2 cut(s) 300, 801
Alw44I GTGCAC 1 cut(s) 370
AlwNI CAGNNNCTG 1 cut(s) 443
Aor13HI TCCGGA 1 cut(s) 417
ApaLI GTGCAC 1 cut(s) 370
ApeKI GCWGC 3 cut(s) 206, 323, 443
ApoI RAATTY 2 cut(s) 177, 470
AseI ATTAAT 1 cut(s) 449
Asp700I GAANNNNTTC 2 cut(s) 255, 735
AsuHPI GGTGA 4 cut(s) 141, 256, 692, 767
AsuNHI GCTAGC 1 cut(s) 457
BaeGI GKGCMC 1 cut(s) 374
BanI GGYRCC 1 cut(s) 479
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbsI GAAGAC 2 cut(s) 258, 828
Bbv12I GWGCWC 1 cut(s) 374
BbvI GCAGC 3 cut(s) 193, 310, 430
BccI CCATC 1 cut(s) 460
BceAI ACGGC 1 cut(s) 75
BciT130I CCWGG 1 cut(s) 586
BcoDI GTCTC 2 cut(s) 300, 801
BfaI CTAG 2 cut(s) 182, 458
BfuAI ACCTGC 1 cut(s) 428
BisI GCNGC 5 cut(s) 207, 324, 444, 478, 745
BlpI GCTNAGC 1 cut(s) 210
BlsI GCNGC 5 cut(s) 208, 325, 445, 479, 746
Bme1390I CCNGG 1 cut(s) 586
BmgBI CACGTC 1 cut(s) 369
BmiI GGNNCC 3 cut(s) 481, 582, 846
BmrFI CCNGG 1 cut(s) 586
BmsI GCATC 3 cut(s) 106, 444, 616
BmtI GCTAGC 1 cut(s) 461
BpiI GAAGAC 2 cut(s) 258, 828
Bpu1102I GCTNAGC 1 cut(s) 210
BpuEI CTTGAG 2 cut(s) 107, 707
BsaJI CCNNGG 2 cut(s) 239, 584
BsaWI WCCGGW 1 cut(s) 417
BsaXI ACNNNNNCTCC 2 cut(s) 503, 533
BseAI TCCGGA 1 cut(s) 417
BseBI CCWGG 1 cut(s) 586
BseDI CCNNGG 2 cut(s) 239, 584
BseGI GGATG 3 cut(s) 121, 772, 862
BseMII CTCAG 2 cut(s) 129, 201
BseSI GKGCMC 1 cut(s) 374
BseXI GCAGC 3 cut(s) 193, 310, 430
BshNI GGYRCC 1 cut(s) 479
BsiHKAI GWGCWC 1 cut(s) 374
BsiSI CCGG 1 cut(s) 418
BsmAI GTCTC 2 cut(s) 300, 801
Bsp1286I GDGCHC 1 cut(s) 374
Bsp13I TCCGGA 1 cut(s) 417
Bsp1407I TGTACA 1 cut(s) 312
Bsp1720I GCTNAGC 1 cut(s) 210
BspACI CCGC 3 cut(s) 200, 477, 745
BspCNI CTCAG 2 cut(s) 130, 202
BspEI TCCGGA 1 cut(s) 417
BspLI GGNNCC 3 cut(s) 481, 582, 846
BspMI ACCTGC 1 cut(s) 428
BspOI GCTAGC 1 cut(s) 461
BspT107I GGYRCC 1 cut(s) 479
BsrGI TGTACA 1 cut(s) 312
BssECI CCNNGG 2 cut(s) 239, 584
Bst2UI CCWGG 1 cut(s) 586
Bst4CI ACNGT 5 cut(s) 42, 345, 351, 704, 735
BstAPI GCANNNNNTGC 1 cut(s) 443
BstAUI TGTACA 1 cut(s) 312
BstC8I GCNNGC 4 cut(s) 64, 204, 321, 459
BstDEI CTNAG 4 cut(s) 132, 138, 194, 210
BstDSI CCRYGG 1 cut(s) 239
BstEII GGTNACC 1 cut(s) 147
BstF5I GGATG 3 cut(s) 121, 772, 862
BstMAI GTCTC 2 cut(s) 300, 801
BstMWI GCNNNNNNNGC 2 cut(s) 443, 554
BstNI CCWGG 1 cut(s) 586
BstNSI RCATGY 1 cut(s) 829
BstPI GGTNACC 1 cut(s) 147
BstSCI CCNGG 1 cut(s) 584
BstSLI GKGCMC 1 cut(s) 374
BstV1I GCAGC 3 cut(s) 193, 310, 430
BstV2I GAAGAC 2 cut(s) 258, 828
BtgI CCRYGG 1 cut(s) 239
BtrI CACGTC 1 cut(s) 369
BtsCI GGATG 3 cut(s) 121, 772, 862
BveI ACCTGC 1 cut(s) 428
Cac8I GCNNGC 4 cut(s) 64, 204, 321, 459
CaiI CAGNNNCTG 1 cut(s) 443
Csp6I GTAC 2 cut(s) 313, 637
CviAII CATG 3 cut(s) 434, 565, 826
CviQI GTAC 2 cut(s) 313, 637
DdeI CTNAG 4 cut(s) 132, 138, 194, 210
DraI TTTAAA 1 cut(s) 334
Eco91I GGTNACC 1 cut(s) 147
EcoO65I GGTNACC 1 cut(s) 147
EcoRI GAATTC 2 cut(s) 177, 470
EcoRII CCWGG 1 cut(s) 584
FaeI CATG 3 cut(s) 437, 568, 829
FalI AAGNNNNNCTT 4 cut(s) 215, 247, 657, 689
FatI CATG 3 cut(s) 433, 564, 825
Fnu4HI GCNGC 5 cut(s) 207, 324, 444, 478, 745
FokI GGATG 2 cut(s) 128, 779
Fsp4HI GCNGC 5 cut(s) 207, 324, 444, 478, 745
FspBI CTAG 2 cut(s) 182, 458
GluI GCNGC 5 cut(s) 207, 324, 444, 478, 745
HapII CCGG 1 cut(s) 418
Hin1II CATG 3 cut(s) 437, 568, 829
HincII GTYRAC 1 cut(s) 129
HindII GTYRAC 1 cut(s) 129
HindIII AAGCTT 2 cut(s) 500, 671
HinfI GANTC 2 cut(s) 421, 785
HpaII CCGG 1 cut(s) 418
HphI GGTGA 4 cut(s) 141, 256, 692, 767
Hpy166II GTNNAC 3 cut(s) 129, 313, 372
Hpy188III TCNNGA 4 cut(s) 418, 686, 716, 839
Hpy8I GTNNAC 3 cut(s) 129, 313, 372
HpyAV CCTTC 2 cut(s) 105, 720
HpyCH4III ACNGT 5 cut(s) 42, 345, 351, 704, 735
HpyCH4IV ACGT 1 cut(s) 368
HpyCH4V TGCA 9 cut(s) 38, 277, 372, 437, 446, 529, 557, 623, 629
HpyF10VI GCNNNNNNNGC 2 cut(s) 443, 554
HpyF3I CTNAG 4 cut(s) 132, 138, 194, 210
HpySE526I ACGT 1 cut(s) 368
Hsp92II CATG 3 cut(s) 437, 568, 829
Kpn2I TCCGGA 1 cut(s) 417
LmnI GCTCC 2 cut(s) 167, 240
Lsp1109I GCAGC 3 cut(s) 193, 310, 430
LweI GCATC 3 cut(s) 106, 444, 616
MaeI CTAG 2 cut(s) 182, 458
MaeII ACGT 1 cut(s) 368
MaeIII GTNAC 5 cut(s) 147, 345, 488, 612, 709
MboII GAAGA 3 cut(s) 263, 674, 833
MfeI CAATTG 1 cut(s) 780
MhlI GDGCHC 1 cut(s) 374
MluCI AATT 5 cut(s) 142, 177, 470, 540, 780
MroI TCCGGA 1 cut(s) 417
MroXI GAANNNNTTC 2 cut(s) 255, 735
MseI TTAA 3 cut(s) 333, 377, 449
MslI CAYNNNNRTG 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 477
MspI CCGG 1 cut(s) 418
MspR9I CCNGG 1 cut(s) 586
MunI CAATTG 1 cut(s) 780
MvaI CCWGG 1 cut(s) 586
MwoI GCNNNNNNNGC 2 cut(s) 443, 554
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 3 cut(s) 437, 568, 829
NlaIV GGNNCC 3 cut(s) 481, 582, 846
NmuCI GTSAC 2 cut(s) 147, 612
NspI RCATGY 1 cut(s) 829
PaqCI CACCTGC 1 cut(s) 428
PciI ACATGT 1 cut(s) 825
PdmI GAANNNNTTC 2 cut(s) 255, 735
PfeI GAWTC 2 cut(s) 421, 785
PkrI GCNGC 5 cut(s) 208, 325, 445, 479, 746
PscI ACATGT 1 cut(s) 825
PshBI ATTAAT 1 cut(s) 449
PsiI TTATAA 2 cut(s) 497, 677
Psp6I CCWGG 1 cut(s) 584
PspEI GGTNACC 1 cut(s) 147
PspGI CCWGG 1 cut(s) 584
PspN4I GGNNCC 3 cut(s) 481, 582, 846
PstNI CAGNNNCTG 1 cut(s) 443
RsaI GTAC 2 cut(s) 314, 638
RsaNI GTAC 2 cut(s) 313, 637
RseI CAYNNNNRTG 1 cut(s) 438
SaqAI TTAA 3 cut(s) 333, 377, 449
SatI GCNGC 5 cut(s) 207, 324, 444, 478, 745
ScrFI CCNGG 1 cut(s) 586
SduI GDGCHC 1 cut(s) 374
SfaNI GCATC 3 cut(s) 106, 444, 616
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 2 cut(s) 122, 686
SmoI CTYRAG 2 cut(s) 122, 686
Sse9I AATT 5 cut(s) 142, 177, 470, 540, 780
SsiI CCGC 3 cut(s) 200, 477, 745
SspI AATATT 1 cut(s) 24
SspMI CTAG 2 cut(s) 182, 458
StyD4I CCNGG 1 cut(s) 584
TaaI ACNGT 5 cut(s) 42, 345, 351, 704, 735
TaiI ACGT 1 cut(s) 371
TaqI TCGA 1 cut(s) 362
TasI AATT 5 cut(s) 142, 177, 470, 540, 780
TatI WGTACW 1 cut(s) 312
TauI GCSGC 2 cut(s) 480, 747
TfiI GAWTC 2 cut(s) 421, 785
Tru1I TTAA 3 cut(s) 333, 377, 449
Tru9I TTAA 3 cut(s) 333, 377, 449
TseFI GTSAC 2 cut(s) 147, 612
TseI GCWGC 3 cut(s) 206, 323, 443
Tsp45I GTSAC 2 cut(s) 147, 612
TspDTI ATGAA 4 cut(s) 62, 116, 553, 728
VneI GTGCAC 1 cut(s) 370
VspI ATTAAT 1 cut(s) 449
XapI RAATTY 2 cut(s) 177, 470
XceI RCATGY 1 cut(s) 829
XmnI GAANNNNTTC 2 cut(s) 255, 735
XspI CTAG 2 cut(s) 182, 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.