Rh5DG194800

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008571
Physical Location & Seq
Forward (+)
21599592 .. 21603168
3577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG194800.1

Sequence Viewer

Length: 1278 bp
ATGAGCGCCAAGATTCTCAAGCAGGCTCAGCTTCAGCAGAGGGAGGTCGAGGACGACGAGAATCGAACCCAAAACCCCAACGCGTTATCGGACATCACCGAAGAGCCGCACGGGGTTGAATCTGATGACGACGATGAGGACGACCCCGATGAGTTCGGTGGGTTTCTTGACAATCGCGGCGAGGATGAAGCGTTTAAAGTGACGAACAGGATGACAAAGGAGATTGGGAAGGAGTATGAGAAAGTATTGGGTAAGGCGGCGGTGGAAGAGGATGAGAAATTACTGGATGCATTTCTTTCGAAGGATGCGGGGCGGCAGCTTACTCTTGCAGACCTGATTGTTGCAAGGATTAAAGAGAATGATTCTAATATCACTCCATCAAATGCCAATTCAGATATGAGACCTTTGCCTAAATTGGATGCAGACACCATAGAAATCTTCAAGGGGGTTGGCCAGTTTCTTAAAAAGTACACGGCTGGAAAGGTTCCCAAACCCTTTAAACACATCCCTTCAATGAATCAATGGGAAGATGTCCTATATTTAACTGAGCCTGAGAATTGGTCTCCCAATGCGATGTATCAAGCTACAAGAATTTTTGCTTCCAATTTGACTGCGAAGAAGGTAGAACGTTTCTACAAGCTTGTCTTGCTTCCACGTGTGAGAGAAGACATTCGGAAGAACAAGCGTTTGCATTTTGCCCTTTATCAGGCTTTGAAAAAGTCTCTTTACAAGCCTGCTGCCTTTTTCAAAGGAATATTGCTACCTTTGTGCGAGTCAGGGACGTGCAATCTAAGAGAAGCTGTCATAGTTGGGAGCGTCTTACAAAAGGTTTCCATTCCGCCACTTCATTCAAGTGTTGCAGTAATGAAGCTAGCAGACATGGAATACTGTGGTACAACGAGTTATTTTATAAAGCTTCTTTTGGAGAAAAAGTATGCTTTGCCATATCGTGTAATTGATGCTCTTGTTGGACATTTTATGAGATTTATGAATGAAACAAGGGTAATGCCAGTGATATGGCACCAATCACTTCTTGCATTTGTGGAAAGGTACAAAAATGATATACTGAACGAGGATAAAGATAATCTTAGAGTTCTTCTTCAAACTCAAAAGCACGATCTGGTAACTCGTGAAATAAGAAAGGAGCTAGATAATAGCCGCAGTCGTGGTGAGAAGGAGGATGACCTTATGTCAATTGCTTCTCCAGATTCTGTGATGATTAAACCTGTTGAAGAAGATATGTTTGATATTCCAGAGGTGCCTATGGAGGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

425

Amino Acids

48.72

Weight (kDa)

5.22

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 105 - 392 1.4e-130 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 911
AccB1I GGYRCC 2 cut(s) 1020, 1258
AccII CGCG 2 cut(s) 83, 177
AciI CCGC 8 cut(s) 107, 177, 257, 260, 308, 313, 839, 1159
AclI AACGTT 1 cut(s) 628
AcoI YGGCCR 1 cut(s) 451
AcsI RAATTY 1 cut(s) 591
AcuI CTGAAG 1 cut(s) 17
AcvI CACGTG 1 cut(s) 656
AfaI GTAC 3 cut(s) 470, 895, 1052
AfiI CCNNNNNNNGG 1 cut(s) 706
AflIII ACRYGT 2 cut(s) 81, 655
AgsI TTSAA 8 cut(s) 119, 442, 513, 715, 748, 852, 1103, 1232
AhdI GACNNNNNGTC 1 cut(s) 1189
AjiI CACGTC 1 cut(s) 783
AluBI AGCT 8 cut(s) 31, 319, 584, 640, 800, 871, 916, 1147
AluI AGCT 8 cut(s) 31, 319, 584, 640, 800, 871, 916, 1147
Alw26I GTCTC 3 cut(s) 394, 567, 726
AlwNI CAGNNNCTG 1 cut(s) 1211
AoxI GGCC 1 cut(s) 451
ApeKI GCWGC 2 cut(s) 316, 737
ApoI RAATTY 1 cut(s) 591
Asp700I GAANNNNTTC 1 cut(s) 669
AspLEI GCGC 1 cut(s) 8
AsuHPI GGTGA 2 cut(s) 88, 1181
AsuII TTCGAA 1 cut(s) 299
AsuNHI GCTAGC 1 cut(s) 871
BalI TGGCCA 1 cut(s) 453
BanI GGYRCC 2 cut(s) 1020, 1258
BauI CACGAG 1 cut(s) 1128
BbrPI CACGTG 1 cut(s) 656
BbsI GAAGAC 1 cut(s) 672
BbvI GCAGC 2 cut(s) 328, 724
BccI CCATC 1 cut(s) 385
BceAI ACGGC 1 cut(s) 489
BcgI CGANNNNNNTGC 2 cut(s) 279, 313
BcoDI GTCTC 3 cut(s) 394, 567, 726
BfaI CTAG 2 cut(s) 872, 1148
BfoI RGCGCY 1 cut(s) 9
BisI GCNGC 7 cut(s) 107, 178, 258, 314, 317, 738, 1159
BlpI GCTNAGC 1 cut(s) 27
BlsI GCNGC 7 cut(s) 108, 179, 259, 315, 318, 739, 1160
BmeRI GACNNNNNGTC 1 cut(s) 1189
BmgBI CACGTC 1 cut(s) 783
BmiI GGNNCC 3 cut(s) 486, 1022, 1260
BmsI GCATC 4 cut(s) 277, 295, 409, 949
BmtI GCTAGC 1 cut(s) 875
BpiI GAAGAC 1 cut(s) 672
BpmI CTGGAG 1 cut(s) 1188
Bpu1102I GCTNAGC 1 cut(s) 27
Bpu14I TTCGAA 1 cut(s) 299
BsaAI YACGTR 1 cut(s) 656
BsaI GGTCTC 2 cut(s) 394, 567
BsaXI ACNNNNNCTCC 2 cut(s) 917, 947
Bsc4I CCNNNNNNNGG 1 cut(s) 706
Bse1I ACTGG 3 cut(s) 288, 454, 1010
BseGI GGATG 9 cut(s) 190, 216, 277, 292, 310, 424, 504, 1186, 1276
BseLI CCNNNNNNNGG 1 cut(s) 706
BseMII CTCAG 3 cut(s) 41, 537, 543
BseNI ACTGG 3 cut(s) 288, 454, 1010
BseXI GCAGC 2 cut(s) 328, 724
Bsh1236I CGCG 2 cut(s) 83, 177
BshFI GGCC 1 cut(s) 453
BshNI GGYRCC 2 cut(s) 1020, 1258
BslFI GGGAC 1 cut(s) 793
BslI CCNNNNNNNGG 1 cut(s) 706
BsmAI GTCTC 3 cut(s) 394, 567, 726
BsmFI GGGAC 1 cut(s) 793
BsnI GGCC 1 cut(s) 453
Bso31I GGTCTC 2 cut(s) 394, 567
Bsp119I TTCGAA 1 cut(s) 299
Bsp143I GATC 1 cut(s) 1117
Bsp1720I GCTNAGC 1 cut(s) 27
BspACI CCGC 8 cut(s) 107, 177, 257, 260, 308, 313, 839, 1159
BspANI GGCC 1 cut(s) 453
BspCNI CTCAG 3 cut(s) 40, 538, 544
BspFNI CGCG 2 cut(s) 83, 177
BspLI GGNNCC 3 cut(s) 486, 1022, 1260
BspOI GCTAGC 1 cut(s) 875
BspQI GCTCTTC 1 cut(s) 96
BspT104I TTCGAA 1 cut(s) 299
BspT107I GGYRCC 2 cut(s) 1020, 1258
BspTNI GGTCTC 2 cut(s) 394, 567
BsrI ACTGG 3 cut(s) 288, 454, 1010
BssMI GATC 1 cut(s) 1117
BssSI CACGAG 1 cut(s) 1128
Bst2BI CACGAG 1 cut(s) 1128
Bst4CI ACNGT 1 cut(s) 890
Bst6I CTCTTC 2 cut(s) 96, 261
BstBAI YACGTR 1 cut(s) 656
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 3 cut(s) 24, 735, 873
BstDEI CTNAG 5 cut(s) 27, 546, 552, 791, 1088
BstENI CCTNNNNNAGG 1 cut(s) 704
BstF5I GGATG 9 cut(s) 190, 216, 277, 292, 310, 424, 504, 1186, 1276
BstFNI CGCG 2 cut(s) 83, 177
BstH2I RGCGCY 1 cut(s) 9
BstHHI GCGC 1 cut(s) 8
BstKTI GATC 1 cut(s) 1120
BstMAI GTCTC 3 cut(s) 394, 567, 726
BstMBI GATC 1 cut(s) 1117
BstMWI GCNNNNNNNGC 2 cut(s) 28, 646
BstUI CGCG 2 cut(s) 83, 177
BstV1I GCAGC 2 cut(s) 328, 724
BstV2I GAAGAC 1 cut(s) 672
BstXI CCANNNNNNTGG 1 cut(s) 1017
BsuRI GGCC 1 cut(s) 453
BtgZI GCGATG 1 cut(s) 587
BtrI CACGTC 1 cut(s) 783
BtsCI GGATG 9 cut(s) 190, 216, 277, 292, 310, 424, 504, 1186, 1276
BtsIMutI CAGTG 1 cut(s) 1017
Cac8I GCNNGC 3 cut(s) 24, 735, 873
CaiI CAGNNNCTG 1 cut(s) 1211
CfoI GCGC 1 cut(s) 8
CseI GACGC 1 cut(s) 805
Csp6I GTAC 3 cut(s) 469, 894, 1051
CviAII CATG 1 cut(s) 880
CviQI GTAC 3 cut(s) 469, 894, 1051
DdeI CTNAG 5 cut(s) 27, 546, 552, 791, 1088
DpnI GATC 1 cut(s) 1119
DpnII GATC 1 cut(s) 1117
DraI TTTAAA 2 cut(s) 196, 499
DriI GACNNNNNGTC 1 cut(s) 1189
EaeI YGGCCR 1 cut(s) 451
Eam1104I CTCTTC 2 cut(s) 96, 261
Eam1105I GACNNNNNGTC 1 cut(s) 1189
EarI CTCTTC 2 cut(s) 96, 261
EciI GGCGGA 1 cut(s) 828
Eco31I GGTCTC 2 cut(s) 394, 567
Eco57I CTGAAG 1 cut(s) 17
Eco72I CACGTG 1 cut(s) 656
EcoNI CCTNNNNNAGG 1 cut(s) 704
EcoT22I ATGCAT 1 cut(s) 292
FaeI CATG 1 cut(s) 883
FalI AAGNNNNNCTT 4 cut(s) 629, 661, 1071, 1103
FaqI GGGAC 1 cut(s) 793
FatI CATG 1 cut(s) 879
FauI CCCGC 1 cut(s) 301
Fnu4HI GCNGC 7 cut(s) 107, 178, 258, 314, 317, 738, 1159
FokI GGATG 8 cut(s) 197, 223, 284, 299, 317, 431, 491, 1193
Fsp4HI GCNGC 7 cut(s) 107, 178, 258, 314, 317, 738, 1159
FspBI CTAG 2 cut(s) 872, 1148
GlaI GCGC 1 cut(s) 7
GluI GCNGC 7 cut(s) 107, 178, 258, 314, 317, 738, 1159
GsuI CTGGAG 1 cut(s) 1188
HaeII RGCGCY 1 cut(s) 9
HaeIII GGCC 1 cut(s) 453
HgaI GACGC 1 cut(s) 805
HhaI GCGC 1 cut(s) 8
Hin1II CATG 1 cut(s) 883
Hin6I GCGC 1 cut(s) 6
HinP1I GCGC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 638, 914
HinfI GANTC 7 cut(s) 13, 61, 119, 362, 517, 773, 1208
HphI GGTGA 2 cut(s) 88, 1181
Hpy166II GTNNAC 1 cut(s) 471
Hpy188I TCNGA 4 cut(s) 91, 124, 394, 675
Hpy188III TCNNGA 4 cut(s) 167, 1130, 1205, 1253
Hpy8I GTNNAC 1 cut(s) 471
Hpy99I CGWCG 2 cut(s) 59, 134
HpyAV CCTTC 5 cut(s) 223, 295, 519, 613, 1168
HpyCH4III ACNGT 1 cut(s) 890
HpyCH4IV ACGT 3 cut(s) 628, 655, 782
HpyCH4V TGCA 8 cut(s) 290, 329, 344, 422, 691, 786, 860, 1037
HpyF10VI GCNNNNNNNGC 2 cut(s) 28, 646
HpyF3I CTNAG 5 cut(s) 27, 546, 552, 791, 1088
HpySE526I ACGT 3 cut(s) 628, 655, 782
Hsp92II CATG 1 cut(s) 883
HspAI GCGC 1 cut(s) 6
Kzo9I GATC 1 cut(s) 1117
LguI GCTCTTC 1 cut(s) 96
LmnI GCTCC 2 cut(s) 813, 1144
Lsp1109I GCAGC 2 cut(s) 328, 724
LweI GCATC 4 cut(s) 277, 295, 409, 949
MaeI CTAG 2 cut(s) 872, 1148
MaeII ACGT 3 cut(s) 628, 655, 782
MaeIII GTNAC 2 cut(s) 199, 1123
MalI GATC 1 cut(s) 1119
MboI GATC 1 cut(s) 1117
MfeI CAATTG 1 cut(s) 1194
MlsI TGGCCA 1 cut(s) 453
MluCI AATT 8 cut(s) 278, 388, 413, 556, 591, 604, 954, 1194
MluI ACGCGT 1 cut(s) 81
MluNI TGGCCA 1 cut(s) 453
MlyI GAGTC 1 cut(s) 782
MmeI TCCRAC 1 cut(s) 949
Mox20I TGGCCA 1 cut(s) 453
Mph1103I ATGCAT 1 cut(s) 292
MroXI GAANNNNTTC 1 cut(s) 669
MscI TGGCCA 1 cut(s) 453
MseI TTAA 6 cut(s) 195, 351, 462, 498, 542, 1221
MslI CAYNNNNRTG 1 cut(s) 852
Msp20I TGGCCA 1 cut(s) 453
MunI CAATTG 1 cut(s) 1194
MvnI CGCG 2 cut(s) 83, 177
MwoI GCNNNNNNNGC 2 cut(s) 28, 646
NdeII GATC 1 cut(s) 1117
NheI GCTAGC 1 cut(s) 871
NlaIII CATG 1 cut(s) 883
NlaIV GGNNCC 3 cut(s) 486, 1022, 1260
NmuCI GTSAC 1 cut(s) 199
NsiI ATGCAT 1 cut(s) 292
NspV TTCGAA 1 cut(s) 299
PciSI GCTCTTC 1 cut(s) 96
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 138
PdmI GAANNNNTTC 1 cut(s) 669
PfeI GAWTC 6 cut(s) 13, 61, 119, 362, 517, 1208
PkrI GCNGC 7 cut(s) 108, 179, 259, 315, 318, 739, 1160
PleI GAGTC 1 cut(s) 781
PmaCI CACGTG 1 cut(s) 656
PmlI CACGTG 1 cut(s) 656
PpsI GAGTC 1 cut(s) 781
Ppu21I YACGTR 1 cut(s) 656
PsiI TTATAA 1 cut(s) 911
Psp1406I AACGTT 1 cut(s) 628
PspCI CACGTG 1 cut(s) 656
PspN4I GGNNCC 3 cut(s) 486, 1022, 1260
PstNI CAGNNNCTG 1 cut(s) 1211
RsaI GTAC 3 cut(s) 470, 895, 1052
RsaNI GTAC 3 cut(s) 469, 894, 1051
RseI CAYNNNNRTG 1 cut(s) 852
SapI GCTCTTC 1 cut(s) 96
SaqAI TTAA 6 cut(s) 195, 351, 462, 498, 542, 1221
SatI GCNGC 7 cut(s) 107, 178, 258, 314, 317, 738, 1159
Sau3AI GATC 1 cut(s) 1117
SchI GAGTC 1 cut(s) 782
SfaNI GCATC 4 cut(s) 277, 295, 409, 949
SfuI TTCGAA 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 1 cut(s) 17
SmoI CTYRAG 1 cut(s) 17
Sse9I AATT 8 cut(s) 278, 388, 413, 556, 591, 604, 954, 1194
SsiI CCGC 8 cut(s) 107, 177, 257, 260, 308, 313, 839, 1159
SspI AATATT 1 cut(s) 756
SspMI CTAG 2 cut(s) 872, 1148
TaaI ACNGT 1 cut(s) 890
TaiI ACGT 3 cut(s) 631, 658, 785
TaqI TCGA 3 cut(s) 48, 64, 299
TasI AATT 8 cut(s) 278, 388, 413, 556, 591, 604, 954, 1194
TatI WGTACW 1 cut(s) 468
TauI GCSGC 5 cut(s) 109, 180, 260, 316, 1161
TfiI GAWTC 6 cut(s) 13, 61, 119, 362, 517, 1208
Tru1I TTAA 6 cut(s) 195, 351, 462, 498, 542, 1221
Tru9I TTAA 6 cut(s) 195, 351, 462, 498, 542, 1221
TscAI CASTG 1 cut(s) 1017
TseFI GTSAC 1 cut(s) 199
TseI GCWGC 2 cut(s) 316, 737
Tsp45I GTSAC 1 cut(s) 199
TspDTI ATGAA 6 cut(s) 201, 530, 836, 881, 1004, 1008
TspRI CASTG 1 cut(s) 1017
XagI CCTNNNNNAGG 1 cut(s) 704
XapI RAATTY 1 cut(s) 591
XmnI GAANNNNTTC 1 cut(s) 669
XspI CTAG 2 cut(s) 872, 1148
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.