FvH4_3g16580

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008571
Physical Location & Seq
Forward (+)
10451702 .. 10455684
3983 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16580.t2

Sequence Viewer

Length: 1422 bp
ATGCCGAAGAAGAGAGGGAGAGAGAAGCACCACAACCCGGAGCCATTTCTGGCCGCGGACGACGACGACGGCGCCTCCGTGGCCACCAAGAAGCGAACCAAGTCCAAGCAGCACCAGACCGACGAGACTCTGATCTCGTCGGGCATGAGCGCCAAGATTCTCAAGCAGGCTCAGCTCCAGCAGAGAGAAGTCGAGGACGACGAGAATCGAGCCCAAAACCCCAACGCGCTGCTCGACATCACCGAGGAGCCAATCGCGGATGAGTCCGATGACGAGGACGAGGACGAGGACGATCCCGATGACTTCGGCGGGTTTCTCGATAATCGCGGTGAGGAGGAAGCGTTTAAAGTGACGAATAGGATGACGAAGGAGATTGGGAAAGAGTATGAGAAGGAGCTAGGTAAGGCGGCGGTGGAGGAGGATGAGAGATTGCTGGATGCGTTTCTTTCCAAGGATGCCGGGCCGCAGCTTACTCTTGCGGACCTGATTGTGGCCAGGATTAAAGAGAATGATTCGAGTATCACTCCATCGAATTCAGATATGCGGCCTTTGCCCAAGCTGGATGCGGACACCATAGAGATCTTTAAGGGGGTTGGCCAGTTTCTGAAAAAGTACACGGCTGGGAAGGTTCCCAAACCCTTTAAACACATCCCTTCAATGAATCAGTGGGAAGGTGTCCTATATTTAACTGAGCCCGAGAATTGGTCTCCAAATGCAATGTATCAAGCTACAAGAATTTTCGCTTCCAATTTGACAGCGAAGAAGGTAGAGCGTTTCTACAAGCTTGTCTTGCTACCGCGTGTGAGAGAAGACATTCGGAAGAACAAGCGCTTGCATTTTGCCCTTTATCAGGCTTTGAAGAAGTCTCTTTACAAGCCCGCTGCCTTTTTCAAAGGAATATTGTTACCTTTGTGTGAGTCAGGAACGTGCAATCTAAGAGAAGCTGTTATAGTTGGGAGCGTCTTACAAAAGGTATCCATTCCGCCACTTCATTCAAGTGTTGCAGCAATGAAGCTGGCAGACATGGAATACTGTGGTACAACGAGTTATTTTATAAAGCTTCTTCTGGAGAAAAAGTATGCTTTGCCATATCGTGTAATTGATGCTCTTGTTGCACATTTCATGAGATTTATGAATGAAACAAGGGTAATGCCAGTGATATGGCACCAATCACTTCTTGCATTTGTGGAAAGGTACAAAAATGATATACTAAACGAGGATAAGGATAACCTTAGAGTTCTTCTTCAAACACAAAGGCATGATCTGGTAACTCGTGAAATAAGAAAAGAGCTAGATAATAGCCGCAGTCGTGGTGAGAAGGCGGATGACCCTATGTCAATTGCTTCTCCAGATTCTGTGATGATTAAGCCTGTTGAAGAAGATATGTTTGATATTCCAGAGGTGCCTATGGAGGATGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

474

Amino Acids

53.98

Weight (kDa)

5.3

Isoelectric Point (pI)

38.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 156 - 440 1.3e-128 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1055
AccB1I GGYRCC 3 cut(s) 71, 1164, 1402
AccII CGCG 5 cut(s) 56, 227, 257, 327, 799
AclWI GGATC 1 cut(s) 287
AcoI YGGCCR 4 cut(s) 51, 81, 492, 595
AcsI RAATTY 2 cut(s) 532, 735
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 3 cut(s) 614, 1039, 1196
AfeI AGCGCT 1 cut(s) 830
AfiI CCNNNNNNNGG 4 cut(s) 37, 490, 702, 850
AgsI TTSAA 6 cut(s) 657, 859, 892, 996, 1247, 1376
AhdI GACNNNNNGTC 1 cut(s) 1333
AjnI CCWGG 1 cut(s) 494
Alw26I GTCTC 3 cut(s) 119, 711, 870
AlwI GGATC 1 cut(s) 287
AlwNI CAGNNNCTG 2 cut(s) 604, 1355
Ama87I CYCGRG 1 cut(s) 695
Aor51HI AGCGCT 1 cut(s) 830
AoxI GGCC 6 cut(s) 51, 81, 461, 492, 545, 595
ApeKI GCWGC 5 cut(s) 109, 229, 466, 881, 1004
ApoI RAATTY 2 cut(s) 532, 735
Asp700I GAANNNNTTC 1 cut(s) 813
AspLEI GCGC 4 cut(s) 74, 152, 229, 831
AspS9I GGNCC 2 cut(s) 461, 481
AsuC2I CCSGG 2 cut(s) 38, 460
AsuHPI GGTGA 3 cut(s) 232, 341, 1325
AvaI CYCGRG 1 cut(s) 695
AvaII GGWCC 1 cut(s) 481
BalI TGGCCA 3 cut(s) 83, 494, 597
BanI GGYRCC 3 cut(s) 71, 1164, 1402
BanII GRGCYC 2 cut(s) 214, 696
BarI GAAGNNNNNNTAC 2 cut(s) 854, 886
BauI CACGAG 1 cut(s) 1272
BbsI GAAGAC 1 cut(s) 816
BbvI GCAGC 5 cut(s) 121, 216, 478, 868, 1016
BccI CCATC 1 cut(s) 535
BceAI ACGGC 2 cut(s) 85, 633
BciT130I CCWGG 1 cut(s) 496
BciVI GTATCC 1 cut(s) 985
BcnI CCSGG 2 cut(s) 38, 460
BcoDI GTCTC 3 cut(s) 119, 711, 870
BfaI CTAG 2 cut(s) 398, 1292
BfoI RGCGCY 3 cut(s) 75, 153, 832
BfuI GTATCC 1 cut(s) 985
BglI GCCNNNNNGGC 1 cut(s) 80
BglII AGATCT 1 cut(s) 579
BlpI GCTNAGC 1 cut(s) 171
Bme1390I CCNGG 3 cut(s) 38, 460, 496
Bme18I GGWCC 1 cut(s) 481
BmeRI GACNNNNNGTC 1 cut(s) 1333
BmeT110I CYCGRG 1 cut(s) 695
BmgT120I GGNCC 2 cut(s) 461, 481
BmiI GGNNCC 6 cut(s) 42, 73, 249, 630, 1166, 1404
BmrFI CCNGG 3 cut(s) 38, 460, 496
BmsI GCATC 4 cut(s) 427, 445, 553, 1093
BpiI GAAGAC 1 cut(s) 816
BplI GAGNNNNNCTC 2 cut(s) 508, 540
BpmI CTGGAG 3 cut(s) 161, 1088, 1332
Bpu1102I GCTNAGC 1 cut(s) 171
BpuEI CTTGAG 1 cut(s) 146
BpuMI CCSGG 2 cut(s) 38, 460
BsaHI GRCGYC 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 711
BsaJI CCNNGG 4 cut(s) 54, 78, 243, 450
BsaXI ACNNNNNCTCC 6 cut(s) 59, 89, 326, 356, 1061, 1091
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 490, 702, 850
Bse1I ACTGG 2 cut(s) 598, 1154
Bse3DI GCAATG 2 cut(s) 723, 1014
BseBI CCWGG 1 cut(s) 496
BseDI CCNNGG 4 cut(s) 54, 78, 243, 450
BseGI GGATG 9 cut(s) 265, 366, 427, 442, 460, 568, 648, 1330, 1420
BseLI CCNNNNNNNGG 4 cut(s) 37, 490, 702, 850
BseMI GCAATG 2 cut(s) 723, 1014
BseMII CTCAG 2 cut(s) 185, 681
BseNI ACTGG 2 cut(s) 598, 1154
BseRI GAGGAG 3 cut(s) 260, 347, 431
BseXI GCAGC 5 cut(s) 121, 216, 478, 868, 1016
BseYI CCCAGC 1 cut(s) 620
Bsh1236I CGCG 5 cut(s) 56, 227, 257, 327, 799
BshFI GGCC 6 cut(s) 53, 83, 463, 494, 547, 597
BshNI GGYRCC 3 cut(s) 71, 1164, 1402
BsiHKCI CYCGRG 1 cut(s) 695
BsiSI CCGG 2 cut(s) 38, 459
BslI CCNNNNNNNGG 4 cut(s) 37, 490, 702, 850
BsmAI GTCTC 3 cut(s) 119, 711, 870
BsnI GGCC 6 cut(s) 53, 83, 463, 494, 547, 597
Bso31I GGTCTC 1 cut(s) 711
BsoBI CYCGRG 1 cut(s) 695
Bsp1286I GDGCHC 2 cut(s) 214, 696
Bsp143I GATC 4 cut(s) 132, 292, 579, 1261
Bsp1720I GCTNAGC 1 cut(s) 171
BspANI GGCC 6 cut(s) 53, 83, 463, 494, 547, 597
BspCNI CTCAG 2 cut(s) 184, 682
BspFNI CGCG 5 cut(s) 56, 227, 257, 327, 799
BspHI TCATGA 1 cut(s) 1122
BspLI GGNNCC 6 cut(s) 42, 73, 249, 630, 1166, 1404
BspPI GGATC 1 cut(s) 287
BspT107I GGYRCC 3 cut(s) 71, 1164, 1402
BspTNI GGTCTC 1 cut(s) 711
BsrDI GCAATG 2 cut(s) 723, 1014
BsrI ACTGG 2 cut(s) 598, 1154
BssECI CCNNGG 4 cut(s) 54, 78, 243, 450
BssMI GATC 4 cut(s) 132, 292, 579, 1261
BssNI GRCGYC 1 cut(s) 72
BssSI CACGAG 1 cut(s) 1272
BssT1I CCWWGG 1 cut(s) 450
Bst2BI CACGAG 1 cut(s) 1272
Bst2UI CCWGG 1 cut(s) 496
Bst4CI ACNGT 1 cut(s) 1034
Bst6I CTCTTC 1 cut(s) 5
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 4 cut(s) 168, 833, 879, 1017
BstDEI CTNAG 4 cut(s) 171, 690, 935, 1232
BstDSI CCRYGG 2 cut(s) 54, 78
BstENI CCTNNNNNAGG 1 cut(s) 848
BstF5I GGATG 9 cut(s) 265, 366, 427, 442, 460, 568, 648, 1330, 1420
BstFNI CGCG 5 cut(s) 56, 227, 257, 327, 799
BstH2I RGCGCY 3 cut(s) 75, 153, 832
BstHHI GCGC 4 cut(s) 74, 152, 229, 831
BstKTI GATC 4 cut(s) 135, 295, 582, 1264
BstMAI GTCTC 3 cut(s) 119, 711, 870
BstMBI GATC 4 cut(s) 132, 292, 579, 1261
BstMWI GCNNNNNNNGC 5 cut(s) 80, 172, 550, 790, 1112
BstNI CCWGG 1 cut(s) 496
BstSCI CCNGG 3 cut(s) 36, 458, 494
BstUI CGCG 5 cut(s) 56, 227, 257, 327, 799
BstV1I GCAGC 5 cut(s) 121, 216, 478, 868, 1016
BstV2I GAAGAC 1 cut(s) 816
BstX2I RGATCY 1 cut(s) 579
BstXI CCANNNNNNTGG 1 cut(s) 1161
BstYI RGATCY 1 cut(s) 579
BsuI GTATCC 1 cut(s) 985
BsuRI GGCC 6 cut(s) 53, 83, 463, 494, 547, 597
BtgI CCRYGG 2 cut(s) 54, 78
BtsCI GGATG 9 cut(s) 265, 366, 427, 442, 460, 568, 648, 1330, 1420
BtsIMutI CAGTG 2 cut(s) 671, 1161
Cac8I GCNNGC 4 cut(s) 168, 833, 879, 1017
CaiI CAGNNNCTG 2 cut(s) 604, 1355
CciI TCATGA 1 cut(s) 1122
CfoI GCGC 4 cut(s) 74, 152, 229, 831
Cfr13I GGNCC 2 cut(s) 461, 481
Cfr42I CCGCGG 1 cut(s) 57
CseI GACGC 1 cut(s) 949
Csp6I GTAC 3 cut(s) 613, 1038, 1195
CviAII CATG 4 cut(s) 145, 1024, 1123, 1259
CviQI GTAC 3 cut(s) 613, 1038, 1195
DdeI CTNAG 4 cut(s) 171, 690, 935, 1232
DinI GGCGCC 1 cut(s) 73
DpnI GATC 4 cut(s) 134, 294, 581, 1263
DpnII GATC 4 cut(s) 132, 292, 579, 1261
DraI TTTAAA 2 cut(s) 346, 643
DriI GACNNNNNGTC 1 cut(s) 1333
EaeI YGGCCR 4 cut(s) 51, 81, 492, 595
Eam1104I CTCTTC 1 cut(s) 5
Eam1105I GACNNNNNGTC 1 cut(s) 1333
EarI CTCTTC 1 cut(s) 5
EciI GGCGGA 2 cut(s) 972, 1337
Eco130I CCWWGG 1 cut(s) 450
Eco24I GRGCYC 2 cut(s) 214, 696
Eco31I GGTCTC 1 cut(s) 711
Eco47I GGWCC 1 cut(s) 481
Eco47III AGCGCT 1 cut(s) 830
Eco88I CYCGRG 1 cut(s) 695
EcoNI CCTNNNNNAGG 1 cut(s) 848
EcoRI GAATTC 1 cut(s) 532
EcoRII CCWGG 1 cut(s) 494
EcoT14I CCWWGG 1 cut(s) 450
EcoT38I GRGCYC 2 cut(s) 214, 696
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 4 cut(s) 148, 1027, 1126, 1262
FalI AAGNNNNNCTT 2 cut(s) 773, 805
FatI CATG 4 cut(s) 144, 1023, 1122, 1258
FauI CCCGC 2 cut(s) 302, 886
FokI GGATG 8 cut(s) 272, 373, 434, 449, 467, 575, 635, 1337
FriOI GRGCYC 2 cut(s) 214, 696
FspBI CTAG 2 cut(s) 398, 1292
GlaI GCGC 4 cut(s) 73, 151, 228, 830
GsaI CCCAGC 1 cut(s) 624
GsuI CTGGAG 3 cut(s) 161, 1088, 1332
HaeII RGCGCY 3 cut(s) 75, 153, 832
HaeIII GGCC 6 cut(s) 53, 83, 463, 494, 547, 597
HapII CCGG 2 cut(s) 38, 459
HgaI GACGC 1 cut(s) 949
HhaI GCGC 4 cut(s) 74, 152, 229, 831
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 4 cut(s) 148, 1027, 1126, 1262
Hin6I GCGC 4 cut(s) 72, 150, 227, 829
HinP1I GCGC 4 cut(s) 72, 150, 227, 829
HindIII AAGCTT 2 cut(s) 782, 1058
HinfI GANTC 8 cut(s) 127, 157, 205, 263, 512, 661, 917, 1352
HpaII CCGG 2 cut(s) 38, 459
HphI GGTGA 3 cut(s) 232, 341, 1325
Hpy166II GTNNAC 1 cut(s) 615
Hpy188I TCNGA 5 cut(s) 132, 268, 538, 606, 819
Hpy188III TCNNGA 8 cut(s) 296, 317, 921, 1067, 1123, 1274, 1349, 1397
Hpy8I GTNNAC 1 cut(s) 615
Hpy99I CGWCG 6 cut(s) 65, 68, 71, 125, 142, 203
HpyAV CCTTC 7 cut(s) 361, 385, 619, 663, 665, 757, 1312
HpyCH4III ACNGT 1 cut(s) 1034
HpyCH4IV ACGT 1 cut(s) 926
HpyCH4V TGCA 6 cut(s) 716, 835, 930, 1004, 1115, 1181
HpyF10VI GCNNNNNNNGC 5 cut(s) 80, 172, 550, 790, 1112
HpyF3I CTNAG 4 cut(s) 171, 690, 935, 1232
HpySE526I ACGT 1 cut(s) 926
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 4 cut(s) 148, 1027, 1126, 1262
HspAI GCGC 4 cut(s) 72, 150, 227, 829
KasI GGCGCC 1 cut(s) 71
KspI CCGCGG 1 cut(s) 57
Kzo9I GATC 4 cut(s) 132, 292, 579, 1261
LmnI GCTCC 5 cut(s) 40, 180, 247, 394, 957
Lsp1109I GCAGC 5 cut(s) 121, 216, 478, 868, 1016
LweI GCATC 4 cut(s) 427, 445, 553, 1093
MaeI CTAG 2 cut(s) 398, 1292
MaeII ACGT 1 cut(s) 926
MaeIII GTNAC 3 cut(s) 349, 903, 1267
MalI GATC 4 cut(s) 134, 294, 581, 1263
MboI GATC 4 cut(s) 132, 292, 579, 1261
MfeI CAATTG 1 cut(s) 1338
MflI RGATCY 1 cut(s) 579
MhlI GDGCHC 2 cut(s) 214, 696
MlsI TGGCCA 3 cut(s) 83, 494, 597
MluCI AATT 6 cut(s) 532, 700, 735, 748, 1098, 1338
MluNI TGGCCA 3 cut(s) 83, 494, 597
Mly113I GGCGCC 1 cut(s) 72
MlyI GAGTC 3 cut(s) 121, 272, 926
Mox20I TGGCCA 3 cut(s) 83, 494, 597
MroXI GAANNNNTTC 1 cut(s) 813
MscI TGGCCA 3 cut(s) 83, 494, 597
MseI TTAA 6 cut(s) 345, 501, 585, 642, 686, 1365
MslI CAYNNNNRTG 1 cut(s) 996
Msp20I TGGCCA 3 cut(s) 83, 494, 597
MspA1I CMGCKG 2 cut(s) 56, 881
MspI CCGG 2 cut(s) 38, 459
MspR9I CCNGG 3 cut(s) 38, 460, 496
MunI CAATTG 1 cut(s) 1338
MvaI CCWGG 1 cut(s) 496
MvnI CGCG 5 cut(s) 56, 227, 257, 327, 799
MwoI GCNNNNNNNGC 5 cut(s) 80, 172, 550, 790, 1112
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 2 cut(s) 38, 460
NdeII GATC 4 cut(s) 132, 292, 579, 1261
NlaIII CATG 4 cut(s) 148, 1027, 1126, 1262
NlaIV GGNNCC 6 cut(s) 42, 73, 249, 630, 1166, 1404
NmuCI GTSAC 1 cut(s) 349
PagI TCATGA 1 cut(s) 1122
PcsI WCGNNNNNNNCGW 4 cut(s) 75, 198, 231, 240
PdmI GAANNNNTTC 1 cut(s) 813
PfeI GAWTC 5 cut(s) 157, 205, 512, 661, 1352
PleI GAGTC 3 cut(s) 121, 271, 925
PluTI GGCGCC 1 cut(s) 75
PpsI GAGTC 3 cut(s) 121, 271, 925
PsiI TTATAA 1 cut(s) 1055
Psp6I CCWGG 1 cut(s) 494
PspFI CCCAGC 1 cut(s) 620
PspGI CCWGG 1 cut(s) 494
PspN4I GGNNCC 6 cut(s) 42, 73, 249, 630, 1166, 1404
PspPI GGNCC 2 cut(s) 461, 481
PstNI CAGNNNCTG 2 cut(s) 604, 1355
PsuI RGATCY 1 cut(s) 579
RsaI GTAC 3 cut(s) 614, 1039, 1196
RsaNI GTAC 3 cut(s) 613, 1038, 1195
RseI CAYNNNNRTG 1 cut(s) 996
SacII CCGCGG 1 cut(s) 57
SaqAI TTAA 6 cut(s) 345, 501, 585, 642, 686, 1365
Sau3AI GATC 4 cut(s) 132, 292, 579, 1261
Sau96I GGNCC 2 cut(s) 461, 481
SchI GAGTC 3 cut(s) 121, 272, 926
ScrFI CCNGG 3 cut(s) 38, 460, 496
SduI GDGCHC 2 cut(s) 214, 696
SfaNI GCATC 4 cut(s) 427, 445, 553, 1093
SfoI GGCGCC 1 cut(s) 73
Sfr303I CCGCGG 1 cut(s) 57
SgrBI CCGCGG 1 cut(s) 57
SinI GGWCC 1 cut(s) 481
SmiMI CAYNNNNRTG 1 cut(s) 996
SmlI CTYRAG 1 cut(s) 161
SmoI CTYRAG 1 cut(s) 161
Sse9I AATT 6 cut(s) 532, 700, 735, 748, 1098, 1338
SspDI GGCGCC 1 cut(s) 71
SspI AATATT 1 cut(s) 900
SspMI CTAG 2 cut(s) 398, 1292
StyD4I CCNGG 3 cut(s) 36, 458, 494
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 1 cut(s) 1034
TaiI ACGT 1 cut(s) 929
TaqI TCGA 6 cut(s) 192, 208, 234, 318, 515, 530
TaqII GACCGA 1 cut(s) 134
TasI AATT 6 cut(s) 532, 700, 735, 748, 1098, 1338
TatI WGTACW 1 cut(s) 612
TauI GCSGC 5 cut(s) 56, 410, 466, 547, 1305
TfiI GAWTC 5 cut(s) 157, 205, 512, 661, 1352
Tru1I TTAA 6 cut(s) 345, 501, 585, 642, 686, 1365
Tru9I TTAA 6 cut(s) 345, 501, 585, 642, 686, 1365
TscAI CASTG 2 cut(s) 671, 1161
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 5 cut(s) 109, 229, 466, 881, 1004
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 6 cut(s) 674, 980, 1025, 1111, 1148, 1152
TspGWI ACGGA 1 cut(s) 67
TspRI CASTG 2 cut(s) 671, 1161
VpaK11BI GGWCC 1 cut(s) 481
XagI CCTNNNNNAGG 1 cut(s) 848
XapI RAATTY 2 cut(s) 532, 735
XmnI GAANNNNTTC 1 cut(s) 813
XspI CTAG 2 cut(s) 398, 1292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.