MD05G1209000.v1.1

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0008571
Physical Location & Seq
Reverse (-)
34081142 .. 34084002
2861 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1209000.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGGGCAAGAAGAGGCAGAGAGAGAGGCACCAGAATCCGGAGCCGTTCCTCGCCGAAGAAAATGGCTCCGTCGCCTCCAAGAAGCGGACCAAGTCGAAGCAGCACCAGCAGGAGGAGAAGCTCATATCTTCCGGAATGAGCTCTAAGATCCTCAAACAGGCTCTGGCTCAGCAGCGGGAGATAGAGGACGAGGAGAACCGCGCTCAAAACCCTAACAATTCGCTTCTGGATGTATCGGAAGAGCCGCGGAAGGAGGTGGCCGACGGCGAAGAGGAGGATATCGATGATTTCGGTGGATTCTCTGAAACGCAGAGCCGATTTGACGGTTATGATGAAAGTGATGAAAATGGCGAGAAGCCATTACATGAGATTGATGAACATGATGAGAAAATATTGGAGCAATTCTTGTCAAAGGATGCCGGTCCGCAGCGTACTCTGGCAGACCTCATTGTCGCAAGAATTAAAGAGAACGATTCAAATGTCGGCTCAGATGCGAGGCCTCTGCCTAAATTGGATACCTCTGTCATAGATTTGTATAAGGGGGTTGGCAAGTTTCTTAATAAGTACACTGCTGGAAAGATTCCCAAGGCATTCAAACACATCCCTTCAGTGCCACGATGGGAGGATGTCCTATATTTGACTGAGCCTGAGAATTGGTCACCAAATGCAATGTATCAAGCTACGAGGATTTTTGCTTCCAATTTGAGTAAGGAGAAGGTAGAACGTTTCTACAAGCTTGTGTTGCTCCCAAGGGTGAGGGAAGACATCCGCAAACCCAAGCGCTTGCATTTTGCACTATATCAATCTTTGAAGAAGTCTTTGTACAAGCCTGCGGCCTTTTTTATAGGAATACTGTTTCCCTTGTGTGAGTCAGGGACATGTAATCTAAGGGAAGCTGTTATTTTTGGGAGCATTATACAGAAGGTCTCCATTCCTCCACTTCATTCTAGTGTTGCATTAATGAAGCTTGCAGAAATGAAATACTGTGGCACAACGAGTTATTTTATAAAGCTTCTTTTGGATAAAAAGTATGCCTTGCCATATCGCGTACTCGACGCTCTTGTTGCACATTTTATGAGATTTATGGAGGAAACAAGAGTAATGCCTGTGATATGGCACCAGTCACTTCTTACATTTGTGCAAAGGTGGGCAAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

387

Amino Acids

44.45

Weight (kDa)

8.18

Isoelectric Point (pI)

45.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 142 - 384 4.8e-113 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1007
AasI GACNNNNNNGTC 1 cut(s) 449
AccB1I GGYRCC 2 cut(s) 27, 1116
AccII CGCG 3 cut(s) 201, 247, 1047
AccIII TCCGGA 2 cut(s) 37, 131
AciI CCGC 8 cut(s) 85, 175, 199, 245, 247, 425, 769, 833
AclI AACGTT 1 cut(s) 724
AclWI GGATC 1 cut(s) 142
AcoI YGGCCR 1 cut(s) 258
AcuI CTGAAG 1 cut(s) 591
AfaI GTAC 4 cut(s) 433, 566, 824, 1050
AfeI AGCGCT 1 cut(s) 782
AfiI CCNNNNNNNGG 4 cut(s) 37, 84, 112, 157
AflIII ACRYGT 1 cut(s) 878
AgsI TTSAA 3 cut(s) 477, 595, 811
AluBI AGCT 7 cut(s) 121, 141, 680, 736, 896, 967, 1012
AluI AGCT 7 cut(s) 121, 141, 680, 736, 896, 967, 1012
Alw21I GWGCWC 1 cut(s) 143
Alw26I GTCTC 1 cut(s) 931
AlwI GGATC 1 cut(s) 142
AlwNI CAGNNNCTG 1 cut(s) 163
Aor13HI TCCGGA 2 cut(s) 37, 131
Aor51HI AGCGCT 1 cut(s) 782
AoxI GGCC 3 cut(s) 258, 497, 834
ApeKI GCWGC 3 cut(s) 100, 172, 427
AseI ATTAAT 1 cut(s) 959
AspLEI GCGC 2 cut(s) 203, 783
AspS9I GGNCC 2 cut(s) 87, 422
AsuHPI GGTGA 2 cut(s) 651, 766
AvaII GGWCC 2 cut(s) 87, 422
BaeI ACNNNNGTAYC 2 cut(s) 507, 540
BanI GGYRCC 2 cut(s) 27, 1116
BanII GRGCYC 1 cut(s) 143
BarI GAAGNNNNNNTAC 2 cut(s) 806, 838
BbsI GAAGAC 1 cut(s) 768
Bbv12I GWGCWC 1 cut(s) 143
BbvI GCAGC 3 cut(s) 112, 184, 439
BccI CCATC 1 cut(s) 612
BceAI ACGGC 2 cut(s) 28, 280
BcgI CGANNNNNNTGC 2 cut(s) 484, 518
BciVI GTATCC 1 cut(s) 508
BcoDI GTCTC 1 cut(s) 931
BfaI CTAG 1 cut(s) 948
BfoI RGCGCY 1 cut(s) 784
BfuI GTATCC 1 cut(s) 508
BisI GCNGC 5 cut(s) 101, 173, 245, 428, 834
BlpI GCTNAGC 1 cut(s) 168
BlsI GCNGC 5 cut(s) 102, 174, 246, 429, 835
Bme18I GGWCC 2 cut(s) 87, 422
BmgT120I GGNCC 2 cut(s) 87, 422
BmiI GGNNCC 4 cut(s) 29, 42, 67, 1118
BmsI GCATC 2 cut(s) 406, 481
BpiI GAAGAC 1 cut(s) 768
Bpu1102I GCTNAGC 1 cut(s) 168
Bsa29I ATCGAT 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 931
BsaJI CCNNGG 3 cut(s) 245, 585, 749
BsaWI WCCGGW 2 cut(s) 37, 131
Bsc4I CCNNNNNNNGG 4 cut(s) 37, 84, 112, 157
Bse118I RCCGGY 1 cut(s) 419
Bse1I ACTGG 1 cut(s) 1120
Bse3DI GCAATG 1 cut(s) 675
BseAI TCCGGA 2 cut(s) 37, 131
BseCI ATCGAT 1 cut(s) 282
BseDI CCNNGG 3 cut(s) 245, 585, 749
BseGI GGATG 5 cut(s) 235, 421, 600, 631, 765
BseLI CCNNNNNNNGG 4 cut(s) 37, 84, 112, 157
BseMI GCAATG 1 cut(s) 675
BseMII CTCAG 4 cut(s) 182, 501, 633, 639
BseNI ACTGG 1 cut(s) 1120
BseRI GAGGAG 3 cut(s) 128, 206, 287
BseXI GCAGC 3 cut(s) 112, 184, 439
Bsh1236I CGCG 3 cut(s) 201, 247, 1047
BshFI GGCC 3 cut(s) 260, 499, 836
BshNI GGYRCC 2 cut(s) 27, 1116
BshVI ATCGAT 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 143
BsiSI CCGG 3 cut(s) 38, 132, 420
BslFI GGGAC 1 cut(s) 889
BslI CCNNNNNNNGG 4 cut(s) 37, 84, 112, 157
BsmAI GTCTC 1 cut(s) 931
BsmFI GGGAC 1 cut(s) 889
BsmI GAATGC 1 cut(s) 590
BsnI GGCC 3 cut(s) 260, 499, 836
Bso31I GGTCTC 1 cut(s) 931
Bsp1286I GDGCHC 1 cut(s) 143
Bsp13I TCCGGA 2 cut(s) 37, 131
Bsp1407I TGTACA 1 cut(s) 822
Bsp143I GATC 1 cut(s) 147
Bsp1720I GCTNAGC 1 cut(s) 168
BspACI CCGC 8 cut(s) 85, 175, 199, 245, 247, 425, 769, 833
BspANI GGCC 3 cut(s) 260, 499, 836
BspCNI CTCAG 4 cut(s) 181, 500, 634, 640
BspDI ATCGAT 1 cut(s) 282
BspEI TCCGGA 2 cut(s) 37, 131
BspFNI CGCG 3 cut(s) 201, 247, 1047
BspLI GGNNCC 4 cut(s) 29, 42, 67, 1118
BspPI GGATC 1 cut(s) 142
BspQI GCTCTTC 1 cut(s) 234
BspT107I GGYRCC 2 cut(s) 27, 1116
BspTNI GGTCTC 1 cut(s) 931
BsrDI GCAATG 1 cut(s) 675
BsrFI RCCGGY 1 cut(s) 419
BsrGI TGTACA 1 cut(s) 822
BsrI ACTGG 1 cut(s) 1120
BssAI RCCGGY 1 cut(s) 419
BssECI CCNNGG 3 cut(s) 245, 585, 749
BssMI GATC 1 cut(s) 147
BssT1I CCWWGG 2 cut(s) 585, 749
Bst4CI ACNGT 3 cut(s) 326, 855, 986
Bst6I CTCTTC 3 cut(s) 5, 234, 264
BstAUI TGTACA 1 cut(s) 822
BstC8I GCNNGC 3 cut(s) 785, 831, 969
BstDEI CTNAG 6 cut(s) 144, 168, 487, 642, 648, 887
BstDSI CCRYGG 1 cut(s) 245
BstEII GGTNACC 1 cut(s) 657
BstENI CCTNNNNNAGG 1 cut(s) 155
BstF5I GGATG 5 cut(s) 235, 421, 600, 631, 765
BstFNI CGCG 3 cut(s) 201, 247, 1047
BstH2I RGCGCY 1 cut(s) 784
BstHHI GCGC 2 cut(s) 203, 783
BstKTI GATC 1 cut(s) 150
BstMAI GTCTC 1 cut(s) 931
BstMBI GATC 1 cut(s) 147
BstMWI GCNNNNNNNGC 3 cut(s) 106, 742, 1064
BstNSI RCATGY 1 cut(s) 882
BstPI GGTNACC 1 cut(s) 657
BstUI CGCG 3 cut(s) 201, 247, 1047
BstV1I GCAGC 3 cut(s) 112, 184, 439
BstV2I GAAGAC 1 cut(s) 768
BstX2I RGATCY 1 cut(s) 147
BstYI RGATCY 1 cut(s) 147
Bsu15I ATCGAT 1 cut(s) 282
BsuI GTATCC 1 cut(s) 508
BsuRI GGCC 3 cut(s) 260, 499, 836
BsuTUI ATCGAT 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 245
BtsCI GGATG 5 cut(s) 235, 421, 600, 631, 765
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 2 cut(s) 567, 615
Cac8I GCNNGC 3 cut(s) 785, 831, 969
CaiI CAGNNNCTG 1 cut(s) 163
CfoI GCGC 2 cut(s) 203, 783
Cfr10I RCCGGY 1 cut(s) 419
Cfr13I GGNCC 2 cut(s) 87, 422
Cfr42I CCGCGG 1 cut(s) 248
ClaI ATCGAT 1 cut(s) 282
CpoI CGGWCCG 1 cut(s) 422
CseI GACGC 1 cut(s) 1064
Csp6I GTAC 4 cut(s) 432, 565, 823, 1049
CspI CGGWCCG 1 cut(s) 422
CviAII CATG 3 cut(s) 365, 380, 879
CviQI GTAC 4 cut(s) 432, 565, 823, 1049
DdeI CTNAG 6 cut(s) 144, 168, 487, 642, 648, 887
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
DrdI GACNNNNNNGTC 1 cut(s) 449
DseDI GACNNNNNNGTC 1 cut(s) 449
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 3 cut(s) 5, 234, 264
EarI CTCTTC 3 cut(s) 5, 234, 264
Ecl136II GAGCTC 1 cut(s) 141
Eco130I CCWWGG 2 cut(s) 585, 749
Eco147I AGGCCT 1 cut(s) 499
Eco24I GRGCYC 1 cut(s) 143
Eco31I GGTCTC 1 cut(s) 931
Eco32I GATATC 1 cut(s) 280
Eco47I GGWCC 2 cut(s) 87, 422
Eco47III AGCGCT 1 cut(s) 782
Eco53kI GAGCTC 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 591
Eco91I GGTNACC 1 cut(s) 657
EcoICRI GAGCTC 1 cut(s) 141
EcoNI CCTNNNNNAGG 1 cut(s) 155
EcoO65I GGTNACC 1 cut(s) 657
EcoRV GATATC 1 cut(s) 280
EcoT14I CCWWGG 2 cut(s) 585, 749
EcoT38I GRGCYC 1 cut(s) 143
ErhI CCWWGG 2 cut(s) 585, 749
FaeI CATG 3 cut(s) 368, 383, 882
FalI AAGNNNNNCTT 2 cut(s) 1019, 1051
FaqI GGGAC 1 cut(s) 889
FatI CATG 3 cut(s) 364, 379, 878
FauI CCCGC 1 cut(s) 168
Fnu4HI GCNGC 5 cut(s) 101, 173, 245, 428, 834
FokI GGATG 5 cut(s) 242, 428, 587, 638, 752
FriOI GRGCYC 1 cut(s) 143
Fsp4HI GCNGC 5 cut(s) 101, 173, 245, 428, 834
FspBI CTAG 1 cut(s) 948
GlaI GCGC 2 cut(s) 202, 782
GluI GCNGC 5 cut(s) 101, 173, 245, 428, 834
HaeII RGCGCY 1 cut(s) 784
HaeIII GGCC 3 cut(s) 260, 499, 836
HapII CCGG 3 cut(s) 38, 132, 420
HgaI GACGC 1 cut(s) 1064
HhaI GCGC 2 cut(s) 203, 783
Hin1II CATG 3 cut(s) 368, 383, 882
Hin6I GCGC 2 cut(s) 201, 781
HinP1I GCGC 2 cut(s) 201, 781
HindIII AAGCTT 3 cut(s) 734, 965, 1010
HinfI GANTC 5 cut(s) 34, 297, 473, 580, 869
HpaII CCGG 3 cut(s) 38, 132, 420
HphI GGTGA 2 cut(s) 651, 766
Hpy166II GTNNAC 1 cut(s) 567
Hpy188I TCNGA 3 cut(s) 238, 304, 490
Hpy188III TCNNGA 3 cut(s) 38, 132, 227
Hpy8I GTNNAC 1 cut(s) 567
Hpy99I CGWCG 3 cut(s) 74, 266, 1058
HpyAV CCTTC 4 cut(s) 244, 615, 709, 916
HpyCH4III ACNGT 3 cut(s) 326, 855, 986
HpyCH4IV ACGT 1 cut(s) 724
HpyCH4V TGCA 7 cut(s) 668, 787, 794, 956, 971, 1067, 1141
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 742, 1064
HpyF3I CTNAG 6 cut(s) 144, 168, 487, 642, 648, 887
HpySE526I ACGT 1 cut(s) 724
Hsp92II CATG 3 cut(s) 368, 383, 882
HspAI GCGC 2 cut(s) 201, 781
Kpn2I TCCGGA 2 cut(s) 37, 131
KspI CCGCGG 1 cut(s) 248
Kzo9I GATC 1 cut(s) 147
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 5 cut(s) 40, 71, 397, 750, 909
Lsp1109I GCAGC 3 cut(s) 112, 184, 439
LweI GCATC 2 cut(s) 406, 481
MaeI CTAG 1 cut(s) 948
MaeII ACGT 1 cut(s) 724
MaeIII GTNAC 2 cut(s) 657, 1122
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 7 cut(s) 22, 68, 120, 251, 281, 773, 823
MflI RGATCY 1 cut(s) 147
MhlI GDGCHC 1 cut(s) 143
MluCI AATT 6 cut(s) 217, 401, 459, 509, 652, 700
MlyI GAGTC 1 cut(s) 878
MroI TCCGGA 2 cut(s) 37, 131
MseI TTAA 4 cut(s) 462, 558, 959, 1159
MslI CAYNNNNRTG 1 cut(s) 948
MspA1I CMGCKG 2 cut(s) 175, 247
MspI CCGG 3 cut(s) 38, 132, 420
Mva1269I GAATGC 1 cut(s) 590
MvnI CGCG 3 cut(s) 201, 247, 1047
MwoI GCNNNNNNNGC 3 cut(s) 106, 742, 1064
NdeII GATC 1 cut(s) 147
NlaIII CATG 3 cut(s) 368, 383, 882
NlaIV GGNNCC 4 cut(s) 29, 42, 67, 1118
NmuCI GTSAC 2 cut(s) 657, 1122
NspI RCATGY 1 cut(s) 882
PceI AGGCCT 1 cut(s) 499
PciI ACATGT 1 cut(s) 878
PciSI GCTCTTC 1 cut(s) 234
PctI GAATGC 1 cut(s) 590
PfeI GAWTC 4 cut(s) 34, 297, 473, 580
PflFI GACNNNGTC 1 cut(s) 91
PkrI GCNGC 5 cut(s) 102, 174, 246, 429, 835
PleI GAGTC 1 cut(s) 877
PpsI GAGTC 1 cut(s) 877
PscI ACATGT 1 cut(s) 878
PshBI ATTAAT 1 cut(s) 959
PsiI TTATAA 1 cut(s) 1007
Psp124BI GAGCTC 1 cut(s) 143
Psp1406I AACGTT 1 cut(s) 724
PspEI GGTNACC 1 cut(s) 657
PspN4I GGNNCC 4 cut(s) 29, 42, 67, 1118
PspPI GGNCC 2 cut(s) 87, 422
PstNI CAGNNNCTG 1 cut(s) 163
PsuI RGATCY 1 cut(s) 147
PsyI GACNNNGTC 1 cut(s) 91
RsaI GTAC 4 cut(s) 433, 566, 824, 1050
RsaNI GTAC 4 cut(s) 432, 565, 823, 1049
RseI CAYNNNNRTG 1 cut(s) 948
Rsr2I CGGWCCG 1 cut(s) 422
RsrII CGGWCCG 1 cut(s) 422
SacI GAGCTC 1 cut(s) 143
SacII CCGCGG 1 cut(s) 248
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 4 cut(s) 462, 558, 959, 1159
SatI GCNGC 5 cut(s) 101, 173, 245, 428, 834
Sau3AI GATC 1 cut(s) 147
Sau96I GGNCC 2 cut(s) 87, 422
SchI GAGTC 1 cut(s) 878
SduI GDGCHC 1 cut(s) 143
SfaNI GCATC 2 cut(s) 406, 481
Sfr303I CCGCGG 1 cut(s) 248
SgrBI CCGCGG 1 cut(s) 248
SinI GGWCC 2 cut(s) 87, 422
SmiMI CAYNNNNRTG 1 cut(s) 948
Sse9I AATT 6 cut(s) 217, 401, 459, 509, 652, 700
SseBI AGGCCT 1 cut(s) 499
SsiI CCGC 8 cut(s) 85, 175, 199, 245, 247, 425, 769, 833
SspI AATATT 1 cut(s) 393
SspMI CTAG 1 cut(s) 948
SstI GAGCTC 1 cut(s) 143
StuI AGGCCT 1 cut(s) 499
StyI CCWWGG 2 cut(s) 585, 749
TaaI ACNGT 3 cut(s) 326, 855, 986
TaiI ACGT 1 cut(s) 727
TaqI TCGA 3 cut(s) 95, 282, 1053
TasI AATT 6 cut(s) 217, 401, 459, 509, 652, 700
TatI WGTACW 2 cut(s) 564, 822
TauI GCSGC 2 cut(s) 247, 836
TfiI GAWTC 4 cut(s) 34, 297, 473, 580
Tru1I TTAA 4 cut(s) 462, 558, 959, 1159
Tru9I TTAA 4 cut(s) 462, 558, 959, 1159
TscAI CASTG 2 cut(s) 574, 615
TseFI GTSAC 2 cut(s) 657, 1122
TseI GCWGC 3 cut(s) 100, 172, 427
Tsp45I GTSAC 2 cut(s) 657, 1122
TspDTI ATGAA 6 cut(s) 348, 357, 390, 932, 977, 992
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 2 cut(s) 574, 615
Tth111I GACNNNGTC 1 cut(s) 91
VpaK11BI GGWCC 2 cut(s) 87, 422
VspI ATTAAT 1 cut(s) 959
XagI CCTNNNNNAGG 1 cut(s) 155
XceI RCATGY 1 cut(s) 882
XspI CTAG 1 cut(s) 948
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.