Rw7G030070

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008571
Physical Location & Seq
Forward (+)
42274672 .. 42278814
4143 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G030070.1

Sequence Viewer

Length: 1242 bp
ATGAATAAGAAGAGGGGGAGAGGGAGTTATCGGAATCCGGTGCCATTCTTGAACGATGGCTCCGTGGTCACCCATAAGCGACTTAAAAAGTCCAAAGAGTCCCGCAAGCACAAGGAAGAGTATCTGATCCTATGTTCAGGCATGAGCTCGAAAATCCTCAAGGAAGCTCAGAATCAGCGGAAGGAGATAGACGACGAAGAGGAAAACACAAACGTCTTCACTCAAGAGGATGAAGAAACATATGAAGAAGAAGTCGATGACTTTGATGCATTCCTTGAGATGCATAAAGAGGATGAAGCAAATAAAGTGATGAAGGGGATGATAAACGAGTATGAGGAAGAGAATGAGAAATTACTGGACCGATTCTTATCAAAGGAGGAGGATCTGCCGGACTTCATTCTTGAGACAATTAAGAAGAAAGATTGGAATGTCACCTCAGCAGGTGCAAGGCCTTTGCTTGAAATGGATACCCATTACATGGAAATATTCAAGGGGGTTGCACAGTTTATGAAAACCTACACGGCTGGCAAGATTACCAAACCATTCAAGTTTATCCCTTCAATGAAACAATGGGAGGATGCCCTCAAGTTGACTGAGCCTGAGAATTGGTCACCACATGCTATGTATGGAGCTACACGAATTCTAGCTTCTAATCTAAGTCCGCAAGCTGCTGAGCGTTTCTACAAGTTTGTCTTGCTCCCACGGGTGAGGGAAGACATTCGTAAGAACAGGCGTTTGCATTTTGCTTTGTATCAGTCTCTAAAGAAGTCTGTGTACAAGCACGCCGCCTTTTTTAAAGGAATACTGTTACCGTTGTGTCAGTCGAGGACGTGCACTTTAAGAGAAGCTGTTGTTGTTGGGAGTATCATAACGAAAGTCCGAATTCCTGTAAATAATGCAGGTGCTGCATTAATGATGCTAGCACAGATGGAATTCAGCGGCACCACAAGTTACTTTATAAAGCTTCTTTTGGAGAAAAAGTTTGCTTTGCAATATCGTGTAATTGAGGCCCTTGTTGCACATTTCATGGGATTTTTGGAGGAACCCAGGATAATGCCTGTGATATGGCATCAGTCACTTCTTGCATTTGCACAGAGGTACAAAAATGCTCTACTAAAGGAGGACAAAGATAAGCTTATAAATCTTCTTGAGGCACAAAATCACCGTGAGAAGACATGTTTGATATTCCAGAGGTTCCTATGGAGGATGACTAATTTACTGATAGGTAGGTTGTCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

413

Amino Acids

48.37

Weight (kDa)

9.07

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 125 - 395 8.2e-107 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 959, 1139
AarI CACCTGC 2 cut(s) 431, 890
Acc36I ACCTGC 2 cut(s) 431, 890
AccB1I GGYRCC 2 cut(s) 40, 941
AccB7I CCANNNNNTGG 1 cut(s) 478
AciI CCGC 5 cut(s) 103, 178, 662, 786, 939
AclWI GGATC 2 cut(s) 121, 390
AcsI RAATTY 3 cut(s) 639, 882, 932
AfaI GTAC 2 cut(s) 776, 1100
AfiI CCNNNNNNNGG 1 cut(s) 478
AflIII ACRYGT 1 cut(s) 1175
AgsI TTSAA 5 cut(s) 52, 461, 490, 547, 561
AjiI CACGTC 1 cut(s) 831
AjnI CCWGG 1 cut(s) 1046
AloI GAACNNNNNNTCC 2 cut(s) 44, 76
AluBI AGCT 8 cut(s) 147, 167, 632, 647, 668, 848, 964, 1135
AluI AGCT 8 cut(s) 147, 167, 632, 647, 668, 848, 964, 1135
Alw21I GWGCWC 2 cut(s) 149, 836
Alw26I GTCTC 2 cut(s) 398, 762
Alw44I GTGCAC 1 cut(s) 832
AlwI GGATC 2 cut(s) 121, 390
AlwNI CAGNNNCTG 1 cut(s) 905
AoxI GGCC 2 cut(s) 449, 1008
ApaLI GTGCAC 1 cut(s) 832
ApeKI GCWGC 2 cut(s) 668, 905
ApoI RAATTY 3 cut(s) 639, 882, 932
AseI ATTAAT 1 cut(s) 911
Asp700I GAANNNNTTC 1 cut(s) 717
AspS9I GGNCC 2 cut(s) 358, 1009
AsuHPI GGTGA 5 cut(s) 61, 424, 603, 718, 1154
AsuNHI GCTAGC 1 cut(s) 919
AvaII GGWCC 1 cut(s) 358
BaeGI GKGCMC 1 cut(s) 836
BanI GGYRCC 2 cut(s) 40, 941
BanII GRGCYC 1 cut(s) 149
BarI GAAGNNNNNNTAC 2 cut(s) 758, 790
BbsI GAAGAC 3 cut(s) 208, 720, 1178
Bbv12I GWGCWC 2 cut(s) 149, 836
BbvCI CCTCAGC 1 cut(s) 436
BbvI GCAGC 2 cut(s) 655, 892
BccI CCATC 2 cut(s) 50, 922
BceAI ACGGC 1 cut(s) 537
BciT130I CCWGG 1 cut(s) 1048
BciVI GTATCC 1 cut(s) 460
BcoDI GTCTC 2 cut(s) 398, 762
BfaI CTAG 2 cut(s) 644, 920
BfuAI ACCTGC 2 cut(s) 431, 890
BfuI GTATCC 1 cut(s) 460
BisI GCNGC 4 cut(s) 669, 786, 906, 940
BlpI GCTNAGC 1 cut(s) 672
BlsI GCNGC 4 cut(s) 670, 787, 907, 941
Bme1390I CCNGG 1 cut(s) 1048
Bme18I GGWCC 1 cut(s) 358
BmgBI CACGTC 1 cut(s) 831
BmgT120I GGNCC 2 cut(s) 358, 1009
BmiI GGNNCC 5 cut(s) 42, 61, 943, 1044, 1196
BmrFI CCNGG 1 cut(s) 1048
BmsI GCATC 5 cut(s) 256, 270, 568, 906, 1078
BmtI GCTAGC 1 cut(s) 923
BpiI GAAGAC 3 cut(s) 208, 720, 1178
Bpu10I CCTNAGC 1 cut(s) 436
Bpu1102I GCTNAGC 1 cut(s) 672
BpuEI CTTGAG 6 cut(s) 143, 207, 296, 422, 569, 1169
BsaBI GATNNNNATC 1 cut(s) 367
BsaJI CCNNGG 3 cut(s) 63, 701, 1046
BsaWI WCCGGW 1 cut(s) 37
BsaXI ACNNNNNCTCC 6 cut(s) 10, 40, 44, 74, 965, 995
Bsc4I CCNNNNNNNGG 1 cut(s) 478
Bse1I ACTGG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 367
BseBI CCWGG 1 cut(s) 1048
BseDI CCNNGG 3 cut(s) 63, 701, 1046
BseGI GGATG 5 cut(s) 235, 298, 324, 583, 1212
BseJI GATNNNNATC 1 cut(s) 367
BseLI CCNNNNNNNGG 1 cut(s) 478
BseMII CTCAG 5 cut(s) 182, 450, 585, 591, 663
BseNI ACTGG 1 cut(s) 360
BseRI GAGGAG 1 cut(s) 392
BseSI GKGCMC 1 cut(s) 836
BseXI GCAGC 2 cut(s) 655, 892
BshFI GGCC 2 cut(s) 451, 1010
BshNI GGYRCC 2 cut(s) 40, 941
BsiHKAI GWGCWC 2 cut(s) 149, 836
BsiSI CCGG 2 cut(s) 38, 389
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 1 cut(s) 478
BsmAI GTCTC 2 cut(s) 398, 762
BsmFI GGGAC 1 cut(s) 85
BsmI GAATGC 1 cut(s) 269
BsnI GGCC 2 cut(s) 451, 1010
Bsp1286I GDGCHC 2 cut(s) 149, 836
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 2 cut(s) 126, 382
Bsp1720I GCTNAGC 1 cut(s) 672
BspACI CCGC 5 cut(s) 103, 178, 662, 786, 939
BspANI GGCC 2 cut(s) 451, 1010
BspCNI CTCAG 5 cut(s) 181, 449, 586, 592, 664
BspLI GGNNCC 5 cut(s) 42, 61, 943, 1044, 1196
BspMI ACCTGC 2 cut(s) 431, 890
BspOI GCTAGC 1 cut(s) 923
BspPI GGATC 2 cut(s) 121, 390
BspT107I GGYRCC 2 cut(s) 40, 941
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 1 cut(s) 360
BssECI CCNNGG 3 cut(s) 63, 701, 1046
BssMI GATC 2 cut(s) 126, 382
Bst2UI CCWGG 1 cut(s) 1048
Bst4CI ACNGT 4 cut(s) 504, 807, 813, 1166
Bst6I CTCTTC 4 cut(s) 5, 111, 192, 333
BstAPI GCANNNNNTGC 1 cut(s) 905
BstAUI TGTACA 1 cut(s) 774
BstC8I GCNNGC 5 cut(s) 107, 526, 666, 783, 921
BstDEI CTNAG 6 cut(s) 168, 436, 594, 600, 656, 672
BstDSI CCRYGG 2 cut(s) 63, 701
BstEII GGTNACC 2 cut(s) 67, 609
BstF5I GGATG 5 cut(s) 235, 298, 324, 583, 1212
BstKTI GATC 2 cut(s) 129, 385
BstMAI GTCTC 2 cut(s) 398, 762
BstMBI GATC 2 cut(s) 126, 382
BstMWI GCNNNNNNNGC 2 cut(s) 905, 1016
BstNI CCWGG 1 cut(s) 1048
BstNSI RCATGY 2 cut(s) 620, 1179
BstPI GGTNACC 2 cut(s) 67, 609
BstSCI CCNGG 1 cut(s) 1046
BstSLI GKGCMC 1 cut(s) 836
BstV1I GCAGC 2 cut(s) 655, 892
BstV2I GAAGAC 3 cut(s) 208, 720, 1178
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuI GTATCC 1 cut(s) 460
BsuRI GGCC 2 cut(s) 451, 1010
BtgI CCRYGG 2 cut(s) 63, 701
BtrI CACGTC 1 cut(s) 831
BtsCI GGATG 5 cut(s) 235, 298, 324, 583, 1212
BveI ACCTGC 2 cut(s) 431, 890
Cac8I GCNNGC 5 cut(s) 107, 526, 666, 783, 921
CaiI CAGNNNCTG 1 cut(s) 905
Cfr13I GGNCC 2 cut(s) 358, 1009
Csp6I GTAC 2 cut(s) 775, 1099
CviAII CATG 5 cut(s) 142, 478, 617, 1027, 1176
CviQI GTAC 2 cut(s) 775, 1099
DdeI CTNAG 6 cut(s) 168, 436, 594, 600, 656, 672
DpnI GATC 2 cut(s) 128, 384
DpnII GATC 2 cut(s) 126, 382
DraI TTTAAA 1 cut(s) 796
Eam1104I CTCTTC 4 cut(s) 5, 111, 192, 333
EarI CTCTTC 4 cut(s) 5, 111, 192, 333
Ecl136II GAGCTC 1 cut(s) 147
Eco147I AGGCCT 1 cut(s) 451
Eco24I GRGCYC 1 cut(s) 149
Eco47I GGWCC 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 147
Eco91I GGTNACC 2 cut(s) 67, 609
EcoICRI GAGCTC 1 cut(s) 147
EcoO109I RGGNCCY 1 cut(s) 1009
EcoO65I GGTNACC 2 cut(s) 67, 609
EcoRI GAATTC 3 cut(s) 639, 882, 932
EcoRII CCWGG 1 cut(s) 1046
EcoT22I ATGCAT 2 cut(s) 271, 285
EcoT38I GRGCYC 1 cut(s) 149
FaeI CATG 5 cut(s) 145, 481, 620, 1030, 1179
FalI AAGNNNNNCTT 4 cut(s) 677, 709, 1119, 1151
FaqI GGGAC 1 cut(s) 85
FatI CATG 5 cut(s) 141, 477, 616, 1026, 1175
FauI CCCGC 1 cut(s) 110
FauNDI CATATG 1 cut(s) 241
Fnu4HI GCNGC 4 cut(s) 669, 786, 906, 940
FokI GGATG 5 cut(s) 242, 305, 331, 590, 1219
FriOI GRGCYC 1 cut(s) 149
Fsp4HI GCNGC 4 cut(s) 669, 786, 906, 940
FspBI CTAG 2 cut(s) 644, 920
GluI GCNGC 4 cut(s) 669, 786, 906, 940
HaeIII GGCC 2 cut(s) 451, 1010
HapII CCGG 2 cut(s) 38, 389
Hin1II CATG 5 cut(s) 145, 481, 620, 1030, 1179
HincII GTYRAC 1 cut(s) 591
HindII GTYRAC 1 cut(s) 591
HindIII AAGCTT 2 cut(s) 962, 1133
HinfI GANTC 4 cut(s) 34, 98, 172, 363
HpaII CCGG 2 cut(s) 38, 389
HphI GGTGA 5 cut(s) 61, 424, 603, 718, 1154
Hpy166II GTNNAC 3 cut(s) 591, 775, 834
Hpy188I TCNGA 4 cut(s) 33, 126, 171, 881
Hpy188III TCNNGA 5 cut(s) 49, 224, 401, 1148, 1189
Hpy8I GTNNAC 3 cut(s) 591, 775, 834
Hpy99I CGWCG 1 cut(s) 197
HpyAV CCTTC 3 cut(s) 175, 307, 567
HpyCH4III ACNGT 4 cut(s) 504, 807, 813, 1166
HpyCH4IV ACGT 2 cut(s) 213, 830
HpyF10VI GCNNNNNNNGC 2 cut(s) 905, 1016
HpyF3I CTNAG 6 cut(s) 168, 436, 594, 600, 656, 672
HpySE526I ACGT 2 cut(s) 213, 830
Hsp92II CATG 5 cut(s) 145, 481, 620, 1030, 1179
Kzo9I GATC 2 cut(s) 126, 382
LmnI GCTCC 3 cut(s) 65, 629, 702
Lsp1109I GCAGC 2 cut(s) 655, 892
LweI GCATC 5 cut(s) 256, 270, 568, 906, 1078
MaeI CTAG 2 cut(s) 644, 920
MaeII ACGT 2 cut(s) 213, 830
MaeIII GTNAC 6 cut(s) 67, 430, 609, 807, 950, 1074
MalI GATC 2 cut(s) 128, 384
MboI GATC 2 cut(s) 126, 382
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 2 cut(s) 149, 836
MluCI AATT 8 cut(s) 350, 408, 604, 639, 882, 932, 1002, 1213
MlyI GAGTC 1 cut(s) 107
Mph1103I ATGCAT 2 cut(s) 271, 285
MroXI GAANNNNTTC 1 cut(s) 717
MseI TTAA 5 cut(s) 84, 411, 795, 839, 911
MspA1I CMGCKG 2 cut(s) 178, 939
MspI CCGG 2 cut(s) 38, 389
MspR9I CCNGG 1 cut(s) 1048
Mva1269I GAATGC 1 cut(s) 269
MvaI CCWGG 1 cut(s) 1048
MwoI GCNNNNNNNGC 2 cut(s) 905, 1016
NdeI CATATG 1 cut(s) 241
NdeII GATC 2 cut(s) 126, 382
NheI GCTAGC 1 cut(s) 919
NlaIII CATG 5 cut(s) 145, 481, 620, 1030, 1179
NlaIV GGNNCC 5 cut(s) 42, 61, 943, 1044, 1196
NmuCI GTSAC 4 cut(s) 67, 430, 609, 1074
NsiI ATGCAT 2 cut(s) 271, 285
NspI RCATGY 2 cut(s) 620, 1179
PaqCI CACCTGC 2 cut(s) 431, 890
PceI AGGCCT 1 cut(s) 451
PciI ACATGT 1 cut(s) 1175
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 1 cut(s) 269
PdmI GAANNNNTTC 1 cut(s) 717
PfeI GAWTC 3 cut(s) 34, 172, 363
PflMI CCANNNNNTGG 1 cut(s) 478
PkrI GCNGC 4 cut(s) 670, 787, 907, 941
PleI GAGTC 1 cut(s) 106
PpsI GAGTC 1 cut(s) 106
PscI ACATGT 1 cut(s) 1175
PshBI ATTAAT 1 cut(s) 911
PsiI TTATAA 2 cut(s) 959, 1139
Psp124BI GAGCTC 1 cut(s) 149
Psp6I CCWGG 1 cut(s) 1046
PspEI GGTNACC 2 cut(s) 67, 609
PspGI CCWGG 1 cut(s) 1046
PspN4I GGNNCC 5 cut(s) 42, 61, 943, 1044, 1196
PspPI GGNCC 2 cut(s) 358, 1009
PstNI CAGNNNCTG 1 cut(s) 905
PsuI RGATCY 1 cut(s) 382
RsaI GTAC 2 cut(s) 776, 1100
RsaNI GTAC 2 cut(s) 775, 1099
SacI GAGCTC 1 cut(s) 149
SaqAI TTAA 5 cut(s) 84, 411, 795, 839, 911
SatI GCNGC 4 cut(s) 669, 786, 906, 940
Sau3AI GATC 2 cut(s) 126, 382
Sau96I GGNCC 2 cut(s) 358, 1009
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 1048
SduI GDGCHC 2 cut(s) 149, 836
SfaNI GCATC 5 cut(s) 256, 270, 568, 906, 1078
SinI GGWCC 1 cut(s) 358
SmlI CTYRAG 6 cut(s) 158, 222, 275, 401, 584, 1148
SmoI CTYRAG 6 cut(s) 158, 222, 275, 401, 584, 1148
Sse9I AATT 8 cut(s) 350, 408, 604, 639, 882, 932, 1002, 1213
SseBI AGGCCT 1 cut(s) 451
SsiI CCGC 5 cut(s) 103, 178, 662, 786, 939
SspI AATATT 1 cut(s) 486
SspMI CTAG 2 cut(s) 644, 920
SstI GAGCTC 1 cut(s) 149
StuI AGGCCT 1 cut(s) 451
StyD4I CCNGG 1 cut(s) 1046
TaaI ACNGT 4 cut(s) 504, 807, 813, 1166
TaiI ACGT 2 cut(s) 216, 833
TaqI TCGA 3 cut(s) 149, 255, 824
TaqII GACCGA 1 cut(s) 375
TasI AATT 8 cut(s) 350, 408, 604, 639, 882, 932, 1002, 1213
TatI WGTACW 1 cut(s) 774
TauI GCSGC 2 cut(s) 788, 942
TfiI GAWTC 3 cut(s) 34, 172, 363
Tru1I TTAA 5 cut(s) 84, 411, 795, 839, 911
Tru9I TTAA 5 cut(s) 84, 411, 795, 839, 911
TseFI GTSAC 4 cut(s) 67, 430, 609, 1074
TseI GCWGC 2 cut(s) 668, 905
Tsp45I GTSAC 4 cut(s) 67, 430, 609, 1074
TspDTI ATGAA 9 cut(s) 17, 246, 258, 309, 326, 385, 524, 578, 1015
TspGWI ACGGA 1 cut(s) 52
Van91I CCANNNNNTGG 1 cut(s) 478
VneI GTGCAC 1 cut(s) 832
VpaK11BI GGWCC 1 cut(s) 358
VspI ATTAAT 1 cut(s) 911
XapI RAATTY 3 cut(s) 639, 882, 932
XceI RCATGY 2 cut(s) 620, 1179
XmnI GAANNNNTTC 1 cut(s) 717
XspI CTAG 2 cut(s) 644, 920
Zsp2I ATGCAT 2 cut(s) 271, 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.