RLG00000033033

Bystin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008571
Physical Location & Seq
Forward (+)
22409179 .. 22442412
33234 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033033

Sequence Viewer

Length: 1494 bp
ATGCCGAAGAAGAGAGGGAGAGAGAGGCATCACAACCCGGAGCCATTTCTGGCCGACGACGATGGCGCCTCTGTTGCCTCCAAGAAGCGGACCAAGTCCAAGCAGCACCAGACCGACGAGACTCTGATTTCGTCGGGCATGAGCGCCAAGATTCTGAAGCAGGCTCAGCTTCAGCAGAGGGAGGTCGAGGACGACGAGAATCGAACCCAAAACCCCAACGCGTTATCGGACATCACCGAGGAGCCGCACGGGGTTGAATCTGATGACGACGATGAGGACGACCCCGATGAGTTTGGTGGGTTTCTTGACAATCGCGGCGAGGATGAAGCGTTTAAAGTGACGAACAGGATGACAAAGGAGATTGGGAAGGAGTATGAGAAAGTATTAGGGAAGGCGGCGGTGGAAGAGGATGAGAAATTACTGGATGCATTTCTTTCGAAGGACGCGGGGCGGCAGCTTACTCTTGCAGACCTGATTGTTGCGAGGATTAAAGAGAATGATTCTAATATCACTCCATCAAATGCCAATTCAGCTGTTGGGTACATCAGAGATGAAATCTGCAAATTTGTATTGGTTGGCCAGTTTCTTAAAAAGTACACGGCTGGAAAGGTTCCCAAACCCTTTAAACACATCCCTTCAATGAATCAATGGGAAGATGTCCTGTATTTAACTGAGCCTGAGAATTGGTCTCCCAATGCGATGTATCAAGCTACAAGAATTTTCGCTTCCAATTTGACTGCGAAGAAGGTAGAACGTTTCTACAAGCTTGTCTTGCTTCCACGTGTGAGAGAAGACATTCGGAAGAACAAGCGCTTGCATTTTGCCCTTTATCAGGCTTTGAAAAAGTCTCTTTACAAGCCTGCTGCCTTTTTCAAAGGAATATTGCTACCTTTGTGCGAGTCAGGGACGTGCAATCTAAGAGAAGCTGTCATAGTTGGGAGCGTCTTACAAAAGGTCTCCATTCCGCCACTTCATTCAAGTGTTGCAGTAATGAAGCTAGCAGACATGGAATACTGTGGTACAACGAGTTATTTTATAAAGCTTCTTTTGGAGAAAAAGTATGCTTTGCCATATCGTGTAATTGATGCTCTTGTTGGACATTTTATGAGATTTATGAATGAAACAAGGGTAATGCCAGTGATATGGCACCAATCACTTCTTGCATTTGTGGAAAGGTACAAAAATGATATACTGAACGAGGATAAAGATAATCTTAGATTCTTCTTCAAACTCAAAAGCACGATCTGCCTGTTGAAGAAGATATGTTTGATATTCCAGAGGTGCCTATGGAGGATGACTAATTTAGTCCTCAATGGTTGGCATAGTTGTCCAATCAAATCTTTCTCCTGGAAGAAGATGCAATGCTGTTCTTTCAGAAGTGATACATTTTTTTTGACCAAATCACAGTTATCTAAAGGCTTCATAATCTTGTTTGTGCATATTCAAATTCGCGTTCAGAGGCACTCTCCTTGCAATGGCAATTTGAACTCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bystin

Protein Analysis

498

Amino Acids

57.16

Weight (kDa)

9.0

Isoelectric Point (pI)

34.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bystin PF05291 152 - 410 1.8e-115 Bystin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1037
AccB1I GGYRCC 3 cut(s) 65, 1146, 1281
AccII CGCG 4 cut(s) 221, 315, 446, 1452
AciI CCGC 8 cut(s) 88, 245, 315, 395, 398, 446, 451, 965
AclI AACGTT 1 cut(s) 754
AcoI YGGCCR 2 cut(s) 51, 577
AcsI RAATTY 3 cut(s) 563, 717, 1446
AcuI CTGAAG 2 cut(s) 155, 176
AcvI CACGTG 1 cut(s) 782
AcyI GRCGYC 1 cut(s) 66
AfaI GTAC 4 cut(s) 542, 596, 1021, 1178
AfeI AGCGCT 1 cut(s) 812
AfiI CCNNNNNNNGG 3 cut(s) 87, 832, 1475
AflIII ACRYGT 2 cut(s) 219, 781
AgsI TTSAA 9 cut(s) 257, 639, 841, 874, 978, 1228, 1255, 1445, 1486
AjiI CACGTC 1 cut(s) 909
AjnI CCWGG 1 cut(s) 1346
AluBI AGCT 8 cut(s) 169, 457, 533, 710, 766, 926, 997, 1042
AluI AGCT 8 cut(s) 169, 457, 533, 710, 766, 926, 997, 1042
Alw26I GTCTC 4 cut(s) 113, 693, 852, 961
Aor51HI AGCGCT 1 cut(s) 812
AoxI GGCC 2 cut(s) 51, 577
ApeKI GCWGC 3 cut(s) 103, 454, 863
ApoI RAATTY 3 cut(s) 563, 717, 1446
ArsI GACNNNNNNTTYG 2 cut(s) 112, 144
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 3 cut(s) 68, 146, 813
AspS9I GGNCC 1 cut(s) 90
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 1 cut(s) 226
AsuII TTCGAA 1 cut(s) 437
AsuNHI GCTAGC 1 cut(s) 997
AvaII GGWCC 1 cut(s) 90
BalI TGGCCA 1 cut(s) 579
BanI GGYRCC 3 cut(s) 65, 1146, 1281
BbrPI CACGTG 1 cut(s) 782
BbsI GAAGAC 1 cut(s) 798
BbvI GCAGC 3 cut(s) 115, 466, 850
BccI CCATC 2 cut(s) 56, 523
BceAI ACGGC 1 cut(s) 615
BcgI CGANNNNNNTGC 2 cut(s) 417, 451
BciT130I CCWGG 1 cut(s) 1348
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 4 cut(s) 113, 693, 852, 961
BfaI CTAG 1 cut(s) 998
BfoI RGCGCY 3 cut(s) 69, 147, 814
BisI GCNGC 7 cut(s) 104, 245, 316, 396, 452, 455, 864
BlpI GCTNAGC 1 cut(s) 165
BlsI GCNGC 7 cut(s) 105, 246, 317, 397, 453, 456, 865
Bme1390I CCNGG 2 cut(s) 38, 1348
Bme18I GGWCC 1 cut(s) 90
BmgBI CACGTC 1 cut(s) 909
BmgT120I GGNCC 1 cut(s) 90
BmiI GGNNCC 6 cut(s) 42, 67, 243, 612, 1148, 1283
BmrFI CCNGG 2 cut(s) 38, 1348
BmsI GCATC 4 cut(s) 37, 415, 1075, 1347
BmtI GCTAGC 1 cut(s) 1001
BpiI GAAGAC 1 cut(s) 798
BplI GAGNNNNNCTC 2 cut(s) 1450, 1482
Bpu1102I GCTNAGC 1 cut(s) 165
Bpu14I TTCGAA 1 cut(s) 437
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 1 cut(s) 782
BsaHI GRCGYC 1 cut(s) 66
BsaI GGTCTC 2 cut(s) 693, 961
BsaJI CCNNGG 1 cut(s) 237
BsaXI ACNNNNNCTCC 2 cut(s) 1043, 1073
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 832, 1475
Bse1I ACTGG 3 cut(s) 426, 580, 1136
Bse3DI GCAATG 2 cut(s) 1367, 1480
BseBI CCWGG 1 cut(s) 1348
BseDI CCNNGG 1 cut(s) 237
BseGI GGATG 6 cut(s) 328, 354, 415, 430, 630, 1299
BseLI CCNNNNNNNGG 3 cut(s) 87, 832, 1475
BseMI GCAATG 2 cut(s) 1367, 1480
BseMII CTCAG 3 cut(s) 179, 663, 669
BseNI ACTGG 3 cut(s) 426, 580, 1136
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 3 cut(s) 115, 466, 850
Bsh1236I CGCG 4 cut(s) 221, 315, 446, 1452
BshFI GGCC 2 cut(s) 53, 579
BshNI GGYRCC 3 cut(s) 65, 1146, 1281
BsiSI CCGG 1 cut(s) 38
BslFI GGGAC 1 cut(s) 919
BslI CCNNNNNNNGG 3 cut(s) 87, 832, 1475
BsmAI GTCTC 4 cut(s) 113, 693, 852, 961
BsmFI GGGAC 1 cut(s) 919
BsnI GGCC 2 cut(s) 53, 579
Bso31I GGTCTC 2 cut(s) 693, 961
Bsp119I TTCGAA 1 cut(s) 437
Bsp143I GATC 1 cut(s) 1242
Bsp1720I GCTNAGC 1 cut(s) 165
BspACI CCGC 8 cut(s) 88, 245, 315, 395, 398, 446, 451, 965
BspANI GGCC 2 cut(s) 53, 579
BspCNI CTCAG 3 cut(s) 178, 664, 670
BspFNI CGCG 4 cut(s) 221, 315, 446, 1452
BspLI GGNNCC 6 cut(s) 42, 67, 243, 612, 1148, 1283
BspOI GCTAGC 1 cut(s) 1001
BspT104I TTCGAA 1 cut(s) 437
BspT107I GGYRCC 3 cut(s) 65, 1146, 1281
BspTNI GGTCTC 2 cut(s) 693, 961
BsrDI GCAATG 2 cut(s) 1367, 1480
BsrI ACTGG 3 cut(s) 426, 580, 1136
BssECI CCNNGG 1 cut(s) 237
BssMI GATC 1 cut(s) 1242
BssNI GRCGYC 1 cut(s) 66
Bst2UI CCWGG 1 cut(s) 1348
Bst4CI ACNGT 2 cut(s) 1016, 1407
Bst6I CTCTTC 2 cut(s) 5, 399
BstACI GRCGYC 1 cut(s) 66
BstAPI GCANNNNNTGC 1 cut(s) 1245
BstBAI YACGTR 1 cut(s) 782
BstBI TTCGAA 1 cut(s) 437
BstC8I GCNNGC 4 cut(s) 162, 815, 861, 999
BstDEI CTNAG 5 cut(s) 165, 672, 678, 917, 1214
BstENI CCTNNNNNAGG 1 cut(s) 830
BstF5I GGATG 6 cut(s) 328, 354, 415, 430, 630, 1299
BstFNI CGCG 4 cut(s) 221, 315, 446, 1452
BstH2I RGCGCY 3 cut(s) 69, 147, 814
BstHHI GCGC 3 cut(s) 68, 146, 813
BstKTI GATC 1 cut(s) 1245
BstMAI GTCTC 4 cut(s) 113, 693, 852, 961
BstMBI GATC 1 cut(s) 1242
BstMWI GCNNNNNNNGC 5 cut(s) 74, 166, 530, 772, 1245
BstNI CCWGG 1 cut(s) 1348
BstSCI CCNGG 2 cut(s) 36, 1346
BstUI CGCG 4 cut(s) 221, 315, 446, 1452
BstV1I GCAGC 3 cut(s) 115, 466, 850
BstV2I GAAGAC 1 cut(s) 798
BstXI CCANNNNNNTGG 1 cut(s) 1143
BsuRI GGCC 2 cut(s) 53, 579
BtgZI GCGATG 1 cut(s) 713
BtrI CACGTC 1 cut(s) 909
BtsCI GGATG 6 cut(s) 328, 354, 415, 430, 630, 1299
BtsIMutI CAGTG 1 cut(s) 1143
Cac8I GCNNGC 4 cut(s) 162, 815, 861, 999
CfoI GCGC 3 cut(s) 68, 146, 813
Cfr13I GGNCC 1 cut(s) 90
CseI GACGC 2 cut(s) 452, 931
Csp6I GTAC 4 cut(s) 541, 595, 1020, 1177
CviAII CATG 2 cut(s) 139, 1006
CviQI GTAC 4 cut(s) 541, 595, 1020, 1177
DdeI CTNAG 5 cut(s) 165, 672, 678, 917, 1214
DinI GGCGCC 1 cut(s) 67
DpnI GATC 1 cut(s) 1244
DpnII GATC 1 cut(s) 1242
DraI TTTAAA 2 cut(s) 334, 625
EaeI YGGCCR 2 cut(s) 51, 577
Eam1104I CTCTTC 2 cut(s) 5, 399
EarI CTCTTC 2 cut(s) 5, 399
EciI GGCGGA 1 cut(s) 954
Eco31I GGTCTC 2 cut(s) 693, 961
Eco47I GGWCC 1 cut(s) 90
Eco47III AGCGCT 1 cut(s) 812
Eco57I CTGAAG 2 cut(s) 155, 176
Eco72I CACGTG 1 cut(s) 782
EcoNI CCTNNNNNAGG 1 cut(s) 830
EcoRII CCWGG 1 cut(s) 1346
EcoT22I ATGCAT 1 cut(s) 430
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
FaeI CATG 2 cut(s) 142, 1009
FalI AAGNNNNNCTT 4 cut(s) 755, 787, 1197, 1229
FaqI GGGAC 1 cut(s) 919
FatI CATG 2 cut(s) 138, 1005
FauI CCCGC 1 cut(s) 439
Fnu4HI GCNGC 7 cut(s) 104, 245, 316, 396, 452, 455, 864
FokI GGATG 6 cut(s) 335, 361, 422, 437, 617, 1306
Fsp4HI GCNGC 7 cut(s) 104, 245, 316, 396, 452, 455, 864
FspBI CTAG 1 cut(s) 998
GlaI GCGC 3 cut(s) 67, 145, 812
GluI GCNGC 7 cut(s) 104, 245, 316, 396, 452, 455, 864
HaeII RGCGCY 3 cut(s) 69, 147, 814
HaeIII GGCC 2 cut(s) 53, 579
HapII CCGG 1 cut(s) 38
HgaI GACGC 2 cut(s) 452, 931
HhaI GCGC 3 cut(s) 68, 146, 813
Hin1I GRCGYC 1 cut(s) 66
Hin1II CATG 2 cut(s) 142, 1009
Hin6I GCGC 3 cut(s) 66, 144, 811
HinP1I GCGC 3 cut(s) 66, 144, 811
HindIII AAGCTT 2 cut(s) 764, 1040
HinfI GANTC 8 cut(s) 121, 151, 199, 257, 500, 643, 899, 1218
HpaII CCGG 1 cut(s) 38
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 1 cut(s) 597
Hpy188I TCNGA 8 cut(s) 126, 156, 229, 262, 548, 801, 1376, 1458
Hpy188III TCNNGA 3 cut(s) 305, 1276, 1491
Hpy8I GTNNAC 1 cut(s) 597
Hpy99I CGWCG 6 cut(s) 59, 62, 119, 136, 197, 272
HpyAV CCTTC 5 cut(s) 361, 385, 433, 645, 739
HpyCH4III ACNGT 2 cut(s) 1016, 1407
HpyCH4IV ACGT 3 cut(s) 754, 781, 908
HpyF10VI GCNNNNNNNGC 5 cut(s) 74, 166, 530, 772, 1245
HpyF3I CTNAG 5 cut(s) 165, 672, 678, 917, 1214
HpySE526I ACGT 3 cut(s) 754, 781, 908
Hsp92I GRCGYC 1 cut(s) 66
Hsp92II CATG 2 cut(s) 142, 1009
HspAI GCGC 3 cut(s) 66, 144, 811
KasI GGCGCC 1 cut(s) 65
Kzo9I GATC 1 cut(s) 1242
LmnI GCTCC 3 cut(s) 40, 241, 939
Lsp1109I GCAGC 3 cut(s) 115, 466, 850
LweI GCATC 4 cut(s) 37, 415, 1075, 1347
MaeI CTAG 1 cut(s) 998
MaeII ACGT 3 cut(s) 754, 781, 908
MaeIII GTNAC 1 cut(s) 337
MalI GATC 1 cut(s) 1244
MboI GATC 1 cut(s) 1242
MlsI TGGCCA 1 cut(s) 579
MluI ACGCGT 1 cut(s) 219
MluNI TGGCCA 1 cut(s) 579
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 2 cut(s) 115, 908
MmeI TCCRAC 1 cut(s) 1075
Mox20I TGGCCA 1 cut(s) 579
Mph1103I ATGCAT 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 795
MscI TGGCCA 1 cut(s) 579
MseI TTAA 5 cut(s) 333, 489, 588, 624, 668
MslI CAYNNNNRTG 1 cut(s) 978
Msp20I TGGCCA 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 533
MspI CCGG 1 cut(s) 38
MspR9I CCNGG 2 cut(s) 38, 1348
MvaI CCWGG 1 cut(s) 1348
MvnI CGCG 4 cut(s) 221, 315, 446, 1452
MwoI GCNNNNNNNGC 5 cut(s) 74, 166, 530, 772, 1245
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 1 cut(s) 38
NdeII GATC 1 cut(s) 1242
NheI GCTAGC 1 cut(s) 997
NlaIII CATG 2 cut(s) 142, 1009
NlaIV GGNNCC 6 cut(s) 42, 67, 243, 612, 1148, 1283
NmuCI GTSAC 1 cut(s) 337
NsiI ATGCAT 1 cut(s) 430
NspV TTCGAA 1 cut(s) 437
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 276
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 6 cut(s) 151, 199, 257, 500, 643, 1218
PflFI GACNNNGTC 1 cut(s) 94
PfoI TCCNGGA 1 cut(s) 1346
PkrI GCNGC 7 cut(s) 105, 246, 317, 397, 453, 456, 865
PleI GAGTC 2 cut(s) 115, 907
PluTI GGCGCC 1 cut(s) 69
PmaCI CACGTG 1 cut(s) 782
PmlI CACGTG 1 cut(s) 782
PpsI GAGTC 2 cut(s) 115, 907
Ppu21I YACGTR 1 cut(s) 782
PsiI TTATAA 1 cut(s) 1037
Psp1406I AACGTT 1 cut(s) 754
Psp6I CCWGG 1 cut(s) 1346
PspCI CACGTG 1 cut(s) 782
PspGI CCWGG 1 cut(s) 1346
PspN4I GGNNCC 6 cut(s) 42, 67, 243, 612, 1148, 1283
PspPI GGNCC 1 cut(s) 90
PsyI GACNNNGTC 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 4 cut(s) 542, 596, 1021, 1178
RsaNI GTAC 4 cut(s) 541, 595, 1020, 1177
RseI CAYNNNNRTG 1 cut(s) 978
SaqAI TTAA 5 cut(s) 333, 489, 588, 624, 668
SatI GCNGC 7 cut(s) 104, 245, 316, 396, 452, 455, 864
Sau3AI GATC 1 cut(s) 1242
Sau96I GGNCC 1 cut(s) 90
SchI GAGTC 2 cut(s) 115, 908
ScrFI CCNGG 2 cut(s) 38, 1348
SfaNI GCATC 4 cut(s) 37, 415, 1075, 1347
SfoI GGCGCC 1 cut(s) 67
SfuI TTCGAA 1 cut(s) 437
SinI GGWCC 1 cut(s) 90
SmiMI CAYNNNNRTG 1 cut(s) 978
SsiI CCGC 8 cut(s) 88, 245, 315, 395, 398, 446, 451, 965
SspDI GGCGCC 1 cut(s) 65
SspI AATATT 1 cut(s) 882
SspMI CTAG 1 cut(s) 998
StyD4I CCNGG 2 cut(s) 36, 1346
TaaI ACNGT 2 cut(s) 1016, 1407
TaiI ACGT 3 cut(s) 757, 784, 911
TaqI TCGA 3 cut(s) 186, 202, 437
TaqII GACCGA 1 cut(s) 128
TatI WGTACW 1 cut(s) 594
TauI GCSGC 4 cut(s) 247, 318, 398, 454
TfiI GAWTC 6 cut(s) 151, 199, 257, 500, 643, 1218
Tru1I TTAA 5 cut(s) 333, 489, 588, 624, 668
Tru9I TTAA 5 cut(s) 333, 489, 588, 624, 668
TscAI CASTG 1 cut(s) 1143
TseFI GTSAC 1 cut(s) 337
TseI GCWGC 3 cut(s) 103, 454, 863
Tsp45I GTSAC 1 cut(s) 337
TspDTI ATGAA 8 cut(s) 339, 567, 656, 962, 1007, 1130, 1134, 1411
TspRI CASTG 1 cut(s) 1143
Tth111I GACNNNGTC 1 cut(s) 94
VpaK11BI GGWCC 1 cut(s) 90
XagI CCTNNNNNAGG 1 cut(s) 830
XapI RAATTY 3 cut(s) 563, 717, 1446
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 1 cut(s) 998
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.